www.cyanocost.com
www.cost.eu/essem
Researcher
CYANOCOST – ES 1105 Action
Cyanobacterial blooms and toxins in water resources:
Occurrence, impacts and management
Short Term Scientific Mission (STSM)
Creation of AACC
(Åbo Akademi Culture Collection)
Objectives
Creation of culture collection named AACC (Åbo Akademi Culture Collection) that represents an
important way of biodiversity preservation of a specific region, but also it offers the possibility of
target selection and use of microorganisms in various fields of science and technology.
Methodology
Sampling on sites where cyanobacterial growth has been observed:
lakes Köyliönjärvi, Pyhäjärvi, Vähäjärvi; Åland Islands:
lakes Nätö Hemviken, Brantsböle Träsk, Baltic Sea.
Isolation of individual cells, filaments and colonies by:
-successive multiplication on solid and liquid media,
-separation using a micropipette.
Purification:
-each culture was spread onto 3 universal growth media
to test bacterial and fungal growth.
Identification and determination
-performed by microscopy work.
Cultivation of biomass
-in media with and without nitrogen
-at room temperature
-illumination with 20 W PowerGlo fluorescent lamps
-light cycle 16 h light - 8 h dark.
Preservation
Recommended methods for preservation of axenic cyanobacterial culture is lyophilization and
cryopreservation as the most reliable and safest way of culture preservation for a long period of
time.
Database creation will make all information related to cyanobacterial cultures available and
affordable to individuals and institutions who want to benefit from the collection.
Results
AACC counts 52 strains isolated from water and terrestrial ecosystems. With the identification of
strains to the genus level, it was found that all isolated strains belong to 17 genera:
Chroococcus, Merismopedia, Cylindrospermum, Woronichinia, Geitlerinema, Gleocapsa,
Microcystis, Oscillatoria, Phormidium, Aphanizomenon, Anabaena, Nostoc, Rivularia, Calothrix,
Tolypothrix and Scytonema.
Prof. Dr Zorica Svirčev
Full time professor at Department of
Biology and Ecology
University of Novi Sad
Serbia
Host Organization
Åbo Akademi University,
Department of Biosciences,
Turku, Finland
Dr Jussi Meriluoto
[email protected]
No
CultivatediD
1
065003
Chroococcus dimidiatus
2
065015
Merismopedia warmingiana
3
065025
Chroococcus dimidiatus
Cultivated species
4
065031
Phormidium tenue
5
065063
Cylindrospermum sp.
6
065064
Cylindrospermum sp.
7
065065
Cylindrospermum sp.
8
065067
Woronichinia sp.
9
065068
10
065070
11
065072
12
065073
13
065074
14
065076
15
065079
16
06$080
17
06$081
18
065082
Medium cul tivated
ELISA
MC/N
0D
(µg/L)
Artemia
EliSA
STX
{µ g/L)
Artemia
Artemia
salina
salina
salina
bioassay
bioassa y
bioassa y
(%)
(%)
(%)
48h intracell 48h intracel 48h extrace
(28 d.c) (old culture) (old culture)
f/2-Si,artificial
seawater based
Z8+N ,artificial
seawater based
f/2-Si,artificial
seawater based
f/2-Si,artificial
seawater based
Z8+N, artificial
seawater based
Z8+N, artificial
seawater based
Z8+N, artificial
seawater based
Z8+N, artificial
seawater based
Z8+N, artificial
Woronichinia sp.
seawater based
Phormidium breve
seawater based
Z8+N,artificial
Z8+N,artificial
Phormidium breve
seawater based
Z8+ N,artificial
Phormidium breve
seawater bas ed
Z8+N, artifici al
Phormidium breve
seawater based
Z8+N, artificial
seawater based
Z8+N,artificial
Phormidium tenue
Spirulina subsal sa
seawater based
Z8+N, artificial
Spirulina subsal sa
seawater based
Z8+N, artificial
Spirulina subsalsa
seawater based
Z8+N, artificial
seawater based
Spirulina subsalsa
19
MC
Microcystis aeruginosa
PCC 7806
BG 11N ±
20
D
Anabaena sp.
BG 11 N +
0.2
90.6
21
H
Anabaena sp.
BG 11N +
22
w
Nostoc sp.
BG 1 1N +
0.16
90.6
23
L2
Phormidium sp.
BG 11N +
0.35
11.8
24
B1
Oscillatoria sp.
BG 11 N-
25
Be
Gfoeocapsa sp.
BG 11N +
0.2
0
3.4
26
K
Geitlerinema ionicum
BG 11N +
0.16
18.5
42.6
27
2B
Nostoc sp.
BG 11N +
0.16
10.2
87
5.7
Anabaena sp.
BG 11N +
0.18
1.3
31
14.7
1.3
18.8
5.5
28
3
>5.00
10
0.3
0
14.1
1.9
73.3
48.2
87
7.6
5.5
0
29
30
30
310
BG 11N +
0.31
31
310
Nostoc sp.
BG 11N-
0.15
9.9
14.3
32
31
Anabaena sp.
BG 11N +
0.2
15.6
87
2.5
33
14
Nostoc sp.
BG 11N +
0.2
0.7
12.8
2.2
34
Anabaena sp.
BG 11N +
84.8
Nostoc sp.
BG 11N ·
Anabaena sp.
BG 11N +
0.19
0.16
36
7
Nostoc sp.
BG 11N +
0.2
0.15
37
K2
Phormidium sp.
BG 11 N +
35
14
Anabaena sp.
4
38
KR
39
SK2
5.5
28
11.2
0.15
61.2
5
2.5
51
Nostoc sp.
BG 11 N+
251
Nostoc sp.
BG 11 N-
2$3
Nostoc sp.
BG 11 N -
58
Nostoc sp.
BG 11N-
LClB
Nostoc sp.
BG 11N-
Nostoc sp.
BG 11N +
c2
Anabaena sp.
47
R2
not determined
48
RWl
Nostoc sp.
BG 11N +
49
Tamis
Nostoc sp.
BG 11N-
50
Zobnatic
Phormidium sp.
BG 11 N +
51
Zobnatic
Nostoc sp.
BG 11N-
Nostoc sp.
BG 11N-
52 Backo Gradiste
18.4
27.5
BG 11 N +
42
LC2
5.5
22.1
0
BG 11N +
40
46
0
0
P hormidium sp.
41
45
0
Phormidium sp.
43
44
0.18
0.2
0
90.6
79
90.6
BG 11N-
15
BG 11N +
0.2
0.15
64.7
46.3
0
90.6
68.5
10.2
0
0
3.8
87
5.2
69.6
0.19
0.17
2.2