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FISH Probes, Reagents, Slide Automation
and Imaging Solutions
The complete range of FISH products available from Leica Biosystems
USA Edition
1
Leica Provides Solutions
for your FISH Assay Needs
Genetic changes leading to loss, gain or reorganization of chromosomal material are an important indicator
of inherited or acquired abnormalities and may play a key role in the development of many cancers.
A popular and widely used method to verify or confirm the occurrence of genetic aberrations is
fluorescence in situ hybridization (FISH).
In FISH, specific labeling of chromosomal DNA is achieved by hybridizing a fluorescently labeled probe
to the target area. A fluorescent microscope can then be used to visualize cellular heterogeneity
(in a population) or chromosomal organization (within each cell).
Leica Biosystems supplies many leading FISH laboratories and can offer you a comprehensive range of
best-in-class FISH products for FISH slide preparation, including:
• Sample processing
• Sample preparation and pretreatment
• Probe hybridization
• Stringency washing
• Image acquisition and analysis
The inclusion of products in this catalog does not infer their use in combination. Please refer to the Instructions for Use at: LeicaBiosystems.com for Intended Use for each product.
2
Contents
Kreatech™ FISH Probes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
ThermoBrite® Elite . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
ThermoBrite® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Consumables & Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Ariol®. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
CytoVision® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
* Trademark notice: REPEAT-FREE is a trademark of Johnson & Johnson Corporation. THERMOBRITE, THERMOBRITE ELITE, ARIOL, CYTOVISION, SLIDELINK, TWINSTAR, UNISTAR,
CLEARENE, APEX, KREATECH, LEICA and the Leica logo are trademarks of Leica Biosystems and its affiliates. All rights reserved.
3
Kreatech™ FISH Probes
Next Generation FISH
Kreatech FISH Probes are the latest advancement in DNA in situ hybridization. These repeat-depleted probes
are constructed using proprietary REPEAT-FREE™ technology that is based on subtractive hybridization, which
specifically removes all probe repetitive elements that are dispersed throughout the target area of interest.
Depleting these repeat sequences leads to more specific binding kinetics and makes the need for blocking DNA
obsolete. This results in FISH assays with brighter signals and reduced background noise.
FEATURES
BENEFITS
• Bright colors
• Eases interpretation of results
• Reduced background
• Reduces number of repeat assays
• Ready-to-use
• Simplifies workflow
Conventional FISH Technology
Kreatech FISH Probe REPEAT-FREE Technology
Blocking DNA


+
Kreatech REPEAT-FREE Probe
ASSAY FEATURES
ASSAY FEATURES
• Sub-optimal binding kinetics
• Excellent binding kinetics
• Need for blocking DNA (Cot-1)
• No Blocking DNA
ISSUES ENCOUNTERED
CUSTOMER BENEFITS
• Relatively high background (haze)
• Improved signal to background ratio
• Repeat rate can be high
• Can reduce repeat rate
4
Analyte Specific Reagent (ASR)
Analytical and performance characteristics are not established. For more information on our ASR product volumes, please contact your local
representative or visit LeicaBiosystems.com. For color setting: FISH probes with catalog numbers ending on 415 are labeled blue , 495 are labeled
green, and 550 are labeled red.
Description
Code
Description
Code
3q11
03Q002B495
IGH@ (14q32) v002
14Q002B550
5q11.2
05Q006B495
MAF (16q23)
16Q001B495
13q34
13Q002B495
MAFB (20q12)
20Q002B495
13q34
13Q002C415
MECOM (3q26) Distal-004
03Q004C550
13q34
13Q002C495
MECOM (3q26) Distal-006
03Q006C415
ABL (9q34)
09Q001C415
MECOM (3q26) Proximal-005
03Q005C495
ALK (2p23) distal
02P002B550
MYC (8q24)
08Q002B550
ALK (2p23) proximal
02P001B495
p53 (17p13)
08Q002B550
ATM (11q22)
11Q001B495
PDGFRA (4q12)
04Q004C415
BCL1 (11q13)
11Q002B495
PDGFRB (5q33) distal
05Q001B495
CCND3 (6p21)
06P003B495
PDGFRB (5q33) proximal
05Q002B550
CHIC2 (4q12)
04Q003C550
RET (10q11) distal
10Q001B495
CKS1B (1q21)
01Q001B550
RET (10q11) proximal
10Q002B550
C-MET (7q31)
07Q001B550
ROS1 (6q22) distal
06Q002B495
DLEU (13q14)
13Q001B550
ROS1 (6q22) distal-SV
06Q005B550
DLEU (13q14)
13Q001C415
ROS1 (6q22) proximal
06Q003B495
EVI t(3;3); inv(3) (3q26) distal
03Q004B550
ROS1 (6q22) proximal
06Q003B550
EVI t(3;3); inv(3) (3q26) proximal
03Q003B495
SE 3 (D3Z1)
03C001C550
FGFR1 (8p11)
08P003B550
SE 7 (D7Z1)
07C001C495
FGFR1 (8p12) distal
08P002B550
SE 7 (D7Z1)
07C001B495
FGFR1 (8p12) proximal
08P001B495
SE 8 (D8Z1)
08C001B495
FGFR2 (10q26)
10Q003B550
SE 10 (D10Z1)
10C001B495
FGFR3 (4p16)
04P001B495
SE 12 (D12Z3)
12C001C495
FGFR4 (5q35)
05Q005B550
SE X (DXZ1)
23C001B495
FIP1L1 (4q12)
04Q002C495
SHOX (Xp22)
23P001B550
hTERC (3q26)
03Q001B550
SRD (1p36)
01P001B495
IGH (14q32)
14Q001B550
5
Research Use Only (RUO) for Use on Tissue
For Research Use Only. Not for use in diagnostic procedures. Our RUO Probes in the table below are provided in a 100 μL, 10-test,
ready-to-use format.
Description
Code
Description
Code
ALK (2p23) Break
KI-10747
FKHR (13q14) Break
KI-10716
ALK/EML4 t(2;2); inv(2) Fusion
KI-10746
FUS (16p11) Break
KI-10715
AR (Xq12) / SE X
KI-10720
hTERT (5p15) / 5q31 (tissue)
KI-10709
AURKA (20q13) / 20q11
KI-10721
IGH (14q32) Break (tissue)
KI-10729
AURKB (17p13) / SE 17
KI-10722
MALT (18q21) Break (tissue)
KI-10731
BCL2 (18q21) Break (tissue)
KI-10745
MDM2 (12q15) / SE 12
KI-10717
BCL2/IGH@ t(14;18) Fusion (tissue)
KI-10755
MDM4 (1q32) / SE 1
KI-10736
BCL-6 (3q27) Break (tissue)
KI-10730
MLL (11q23) / SE 11
KI-10711
C-MET (7q31) / SE 7
KI-10719
MYC (8q24) Break, TC (tissue)
KI-10749
CHOP (12q13) Break
KI-10714
MYCN (2p24) / LAF (2q11)
KI-10706
hTERC (3q26) / C-MYC (8q24) / SE 7 TC
KI-10704
p16 (9p21) / 9q21 (tissue)
KI-10710
CCND1 (11q13) / SE 11
KI-10734
p53 (17p13) / SE 17 (tissue)
KI-10738
CDK4 (12q13) / SE 12
KI-10725
PPARy (3p25) Break
KI-10707
FGFR4 (5q35) / 5q11.2
KI-10756
PTEN (10q23) / SE 10
KI-10718
EGFR, Her-1 (7p11) / SE7
KI-10702
RET (10q11) Break
KI-10753
ERBB2, Her-2/Neu (17q12) / SE 17
KI-10701
ROS1 (6q22) Break
KI-10752
ERCC1 (19q13) & ZNF443 (19p13)
KI-10739
SRD (1p36) / SE 1(1qh)
KI-10712
EWSR1 (22q12) Break
KI-10750
SYT (18q11) Break
KI-10713
EWSR1/NFATC2 t(20;22) DC, S-Fusion
KI-10751
TFE3 (Xp11) Break
KI-10741
FGFR1 (8p12) Break
KI-10737
TOP2A (17q21) / SE 17
KI-10724
FGFR1 (8p11) / SE 8 (D8Z1)
KI-10754
TOP2A (17q21) / Her2 (17q12) / SE 17 TC
KI-10735
FGFR2 (10q26) / SE 10 (D10Z1)
KI-10757
TMPRSS2-ERG (21q22) Del, Break, TC
KI-10726
ZNF217 (20q13) / 20q11
KI-10733
RUO CISH Kit
For Research Use Only. Not for use in diagnostic procedures. Our RUO Probes in the table below are provided in a 100 μL, 10-test,
ready-to-use format.
Description
Code
Description
Code
TwinStar™ CISH Detection Kit
KI-60010
UniStar™ CISH Detection Kit
KI-50001
6
RUO for Use on Cells
For Research Use Only. Not for use in diagnostic procedures. Our RUO Probes in the table below are provided in a 100 μL, 10-test,
ready-to-use format.
Description
Code
Description
Code
1q21 / 8p21
KI-10503
hTERC (3q26) / 3q11
KI-10110
1q21 / SRD (1p36)
KI-10507
hTERT (5p15) / 5q31
KI-10208
6q21 / C-MYC (8q24)
KI-10117
IGH (14q32) Break
KI-10601
6q21 / SE 6
KI-10105
IRF4/DUSP22 (6p25) Break
KI-10613
11q23 / DLEU (13q14)
KI-10502
JAK2 (9p24) Break
KI-10012
15q22 / 6q21
KI-10504
MAF/IGH t(14;16) Fusion
KI-10610
15q22 / 9q34
KI-10508
MAFB/IGH@ t(14;20) Fusion
KI-10510
19q13 / p53 (17p13)
KI-10509
MALT (18q21) Break
KI-10608
AML/ETO t(8;21) Fusion
KI-10301
5q- (5q31; 5q33)
KI-10209
ATM (11q22) / SE 11
KI-10103
5q- (5q31; 5q33) / hTERT (5p15) TC
KI-10210
ATM (11q22) / GLI (12q13)
KI-10108
7q- (7q22; 7q36)
KI-10202
BCL1/IGH t(11;14) Fusion
KI-10604
7q- (7q22; 7q36) / SE 7 TC
KI-10207
BCL2 (18q21) Break
KI-10612
20q- (PTPRT 20q12) / 20q11
KI-10203
BCL2/IGH t(14;18) Fusion
KI-10606
MECOM/RUNX1 t(3;21) Fusion
KI-10310
BCL6 (3q27) Break
KI-10607
MLL (11q23) Break
KI-10303
BCR/ABL t(9;22) Fusion
KI-10005
MLL/AFF1 t(4;11)
KI-10404
BCR/ABL t(9;22) TC, D-Fusion
KI-10006
MLL/MLLT1 t(11;19) Fusion
KI-10307
BCR/ABL t(9;22,) DC, S-Fusion, ES
KI-10008
MLL/MLLT3 t(9;11) Fusion
KI-10308
BCR/ABL t(9;22) DC, S-Fusion
KI-10009
MLL/MLLT4 t(6;11) Fusion
KI-10309
GLI (12q13) / SE 12
KI-10104
MYC (8q24) Break, TC
KI-10611
CCND1 (BCL1;11q13) Break
KI-10609
MYC/IGH t(8;14) Fusion
KI-10603
C-MYC (8q24) / SE 8
KI-10106
NUP98 (11p15) Break
KI-10311
DEK / NUP214 t(6;9) Fusion
KI-10306
p16 (9p21) / 9q21
KI-10402
DLEU (13q14) / 13qter
KI-10102
p53 (17p13) / ATM (11q22)
KI-10114
DLEU (13q14) / p53 (17p13)
KI-10113
p53 (17p13) / MPO (17q22) “ISO 17q”
KI-10011
ETV6 (TEL) (12p13) Break
KI-10403
p53 (17p13) / SE 17
KI-10112
EVI t(3;3); inv(3) (3q26) Break
KI-10204
PDGFRB (5q33) Break
KI-10004
7
RUO for Use on Cells (Continued)
For Research Use Only. Not for use in diagnostic procedures. Our RUO Probes in the table below are provided in a 100 μL, 10-test,
ready-to-use format.
Description
Code
Description
Code
EVI t(3;3); inv(3) (3q26) Break, TC
KI-10205
PML/RARA t(15;17) Fusion
KI-10302
FIP1L1-CHIC2-PDGFRA (4q12) Del, Break
KI-10003
PML/RARA t(15;17) Fusion
KI-12302
FGFR3/IGH t(4;14) Fusion
KI-10602
RARA (17q21) Break
KI-10305
FIP1L1-CHIC2-PDGFRA (4q12) Del, Break, TC
KI-10007
TEL/AML t(12;21) Fusion
KI-10401
GLI (12q13) / SE 12
KI-10104
C-MYC (8q24) / SE 8
KI-10106
20q- (PTPRT 20q12) / 20q11
KI-10203
CBFB t(16;16), inv(16) Break
KI-10304
dic(9;20) Triple Color Probe
KI-10405
MYEOV/IGH t(11;14) Fusion
KI-10605
RUO PN FISH Probes
For Research Use Only. Not for use in diagnostic procedures. Our RUO Probes in the table below are provided in a 100 μL, 10-test,
ready-to-use format. except where noted†.
Description
Code
Description
Code
13 (13q14) / 21 (21q22)
KI-40003
21, X, Y (20-test)
KI-40008†
13/21, X/Y18
KI-40005
SE X (DXZ1) / Y (DYZ3) / 18 (D18Z1)
KI-20032
13/21, X/Y18 (30-test)
KI-40006
SE 7 (D7Z1) / SE 8 (D8Z1)
KI-20031
13/21, X/Y18 (50-test)
KI-40007
SE X (DXZ1) / SE Y (DYZ3)
KI-20030
†
†
RUO Microdeletion FISH Probes
For Research Use Only. Not for use in diagnostic procedures. Our RUO Probes in the table below are provided in a 100 μL, 10-test, ready-to-use
format.
Description
Code
Description
Code
Angelman UBE3A (15q11) / PML(15q24)
KI-40110
NF1 (17q11) / MPO (17q22)
KI-40114
Cri-Du-Chat CTNND (5p15) / 5q31
KI-40106
NSD1 (5q35)/ hTERT (5p15)
KI-40113
DiGeorge “N25” (22q11) / 22q13 (SHANK3)
KI-40102
Prader-Willi SNRPN (15q11) / PML(15q24)
KI-40109
DiGeorge Tuple (22q11) / 22q13 (SHANK3)
KI-40103
Short Stature (Xp22) / SE X
KI-40112
DiGeorge T-box1 (22q11) / 22q13 (SHANK3)
KI-40104
STS (Xp22) / KAL (Xp22) / SE X TC
KI-40115
DiGeorge II (10p14) / SE 10
KI-40105
Williams-Beuren ELN (7q11) / 7q22
KI-40111
GATA4 (8p23) / SE 8
KI-40118
Wolf-Hirschhorn WHSC1 (4p16) / SE 4
KI-40107
IGF1R (15q26) / 15q11
KI-40116
X-Inactivation XIST (Xq13) / SE X
KI-40108
Miller-Dieker LIS (17p13 ) / Smith-Magenis RAI (17p11) KI-40101
8
Other FISH Probes
For Research Use Only. Not for use in diagnostic procedures. Our RUO Probes in the table below are provided in a 100 μL, 10-test, ready-to-use
format. Our ASR Probes in the table below are provided either in a 10 or 20 µL, 5x concentrated format. Available in red, green, or blue.
•
Satellite Enumeration Probes - ASR/RUO
•
Whole Chromosome Probes - ASR
•
Sub-Telomeric Probes - ASR
General Purpose Reagents
Description
Code
Description
Code
Rubber Cement, Fixogum
LK-071A
FISH Hybridization Buffer (FHB)
KI-FHB
DAPI Counterstain (0.1µg/ml)
LK-095A
Paraffin Tissue Buffer (PTB)
KI-PTB
DAPI Counterstain (1µg/ml)
LK-096A
Whole Chromosome Buffer (WCB)
KI-WCB
Counterstain Diluent
LK-097A
Pretreatment Solution B (1000 ml)
LK-100C
Wash Buffer (10x Conc.) (250 ml)
LK-141B
Wash Buffer (10x Conc.) (1000 ml)
LK-141C
9
ThermoBrite® Elite
The Solution for Automated FISH Slide Preparation
The ThermoBrite Elite automates and standardizes the FISH slide preparation process including
deparaffinization, pre-treatment, denaturation/hybridization and post hybridization wash. Application of
probe, counterstain and cover slipping are the only manual steps. Just load your slides and walk away.
Minimal hands-on-time frees up technologists for other important tasks.
The ThermoBrite Elite hybridizes with temperature controlled to ± 1°C and can process up to twelve slides
per run with the ability to adapt to smaller batches. For higher throughput, transfer slides to a standard
ThermoBrite instrument to denature/hybridize and continue using your ThermoBrite Elite for additional batch runs.
The intuitive software included enables users to run preloaded protocols or to create up to 1,000 user
defined protocols. The instrument can be programmed to work with virtually any probe or protocol, allowing
the selection of up to ten reagents and three separate waste paths.
FEATURES
BENEFITS
• Bench top unit, 12 slide capacity
• Fast protocol setup and start of run
• Automated fluidics system
• Hands-on-time reduced to 3 steps from >30 (FFPE)
• Hybridization temperature precision to ± 1 °C
• Free up technologists for other tasks
• Workflow based software navigation
• Flexible and easy-to-use
• Open system-preloaded & custom protocols
• Increases laboratory productivity
• Ability to perform post hybridization wash
ThermoBrite® Elite and ThermoBrite® are Class I Medical Device, ETL, CSA and CE Marked for in vitro diagnostic use.
10
ThermoBrite®
Programmable Temperature Controlled Slide Processing System
This programmable system automates the denaturation and hybridization steps in slide-based ISH procedures,
and provides walk-away convenience for clinical and research personnel. The ThermoBrite accepts a wide
range of sample types, is easy to use, and may reduce hands-on time by more than 50% for this step, while
ensuring overall precision and accuracy in FISH assays.
FEATURES
BENEFITS
• 40 user defined protocols and 3 operating modes
• Eliminates manual steps and reduces hands-on time during
• Numeric keypad allows for easy programming
ISH procedures
• Can be used as a fixed temperature slide warmer
• Does not need full batch (12) to maintain temperature accuracy
• Rapid temperature ramp-up and accuracy of ± 1 °C
• Slide guide aligns slides and allows for one hand removal
• Excellent temperature uniformity across all slide positions
• Optimal humidity control
• Heats slide to temperatures ideal for FISH procedures
ThermoBrite Elite (TBE)
ThermoBrite (TB)
Description
Code
Description
Code
ThermoBrite Elite Instrument
3800-007000-001
ThermoBrite Instrument 120v
3800-004852-001
Peri-Tube Kit, 2 Tubes
3800-007742-001
ThermoBrite Instrument 240v
3800-004852-002
Peri-Tube Kit, 12 tubes
3801-010005-001
Temperature Verification Kit
3800-006418-001
TBE Slide Carriers (6)
3800-007744-001
ThermoBrite Calibration/Service
Call (800) 248-0123
ThermoBrite Elite Service Agreement
Call (800) 248-0123
Humidity Cards (10 pk)
3800-004970-001
11
Consumables & Reagents
Leica Biosystems‘ laboratory consumables support your histology/cytology FISH slide prep process,
delivering quality at each step while enabling increased workflow efficiency. Whether your needs are for
glass slides, coverslips, reagent grade alcohol or dewaxing agents, we provide the key consumables to
drive quality results in your laboratory.
Neutral Buffered Formalin Fixatives
A prepared 10% Neutral Phosphate Buffered Formalin (NBF) is available for the histotechnologist who prefers the convenience of having a
ready-to-use fixative at hand. Each batch of fixative is quality control tested in our own histology laboratory to guarantee a consistent neutral pH
range and excellent fixative properties. The 10% Neutral Buffered Formalin is conveniently packaged in a cubitainer with an attached handle and
easy to use "sure shut" dispenser spigot.
Description
Code
Size
10% NBF, Gallon (3.8 L)
3800598
Case / 4
10% NBF, 5 Gallon Cube (18.9 L)
3800600
Each
10% NBF, 2.5 Gallon Cube
3800602
Each
Clearene™ Solvent
Clearene is a selected blend of terpenes formulated specifically to replace xylene and toluene in histology and cytology laboratory procedures.
It is less oily than other solvent substitutes and therefore dries quickly and does not leave a residue. Clearene is biodegradable and offers the
desirable characteristics of a good solvent but not the harmful drawbacks of aromatic hydrocarbons such as xylene and toluene-based products.
Description
Code
Size
Clearene, Gallon (3.8 L)
3803600
Each
Clearene, Gallon (3.8 L)
3803620
Case / 4
Formaldehyde Solution Fixative
This product is made available for those who desire to create their own buffered formulas or where higher than 10% product ratio to water is
required. 40% by volume, 37% by weight, stabilized with methanol to inhibit polymerzation.
Description
Code
Size
Formaldehyde Solution, Gallon (3.8 L)
3800750
Case / 4
Alcohols: Reagent Grade Ethanol, Isopropanol
These Isopropanol and Reagent Alcohols are pure filtered and laboratory tested to ensure a consistent high quality for all formulating, processing
and staining procedures in histology and cytology. The 95% and 100% reagent grade Ethanols are not classified as a controlled substance and
therefore do not require record keeping. Gallon sizes are supplied in non-breakable polybottles.
Description
Code
Size
Ethanol, 95%, Gallon (3.8 L)
3803682
Case / 4
Ethanol, 100%, Gallon (3.8 L)
3803686
Case / 4
Isopropanol, 99%, Gallon (3.8L)
3803660
Case / 4
12
Coverglass and Coverslips
Premier Coverglass specially designed to work with automated cover-slip machines. Extra processes to prevent sticking and additional
packaging protection is included for extra moisture protection. The cover glass comes in four sizes, #1 Thinness: 0.13 mm - 0.17 mm #1.5
Thinness: 0.16 mm - 0.19 mm. Please contact your Leica representative for a complete list of all available Coverglass.
Description
Code
Size
24 x 50 / #1
3800145ACS
Case / 650
24 x 50 / #1
3800145
Case / 620
Coverslips 24x24 mm
14071135634
10 Boxes of 100 Each
Coverslips 22x22 mm
14071135633
10 Boxes of 100 Each
Coverslips 18x18 mm
14071135632
10 Boxes of 100 Each
Apex™ Superior Adhesive Slide
Apex Superior Adhesive Slides are microscope slides made of white glass and coated with a special adhesive material that enables the slide to
be more hydrophilic. This hydrophilicity provides a better base for homogeneous covering by aqueous stains and reagents leading to consistent
full slide staining.
Description
Code
Size
Apex Superior Adhesive Slide, White
3800080
Case / 1,440
Apex Superior Adhesive Slide, Yellow
3800081
Case / 1,440
Apex Superior Adhesive Slide, Blue
3800082
Case / 1,440
Apex Superior Adhesive Slide, Green
3800083
Case / 1,440
Apex Superior Adhesive Slide, Pink
3800084
Case / 1,440
Apex Superior Adhesive Slide, Lavender
3800085
Case / 1,440
Microslides for Cytology funnels
3808380
Box/1000
Microslides for Cytology funnels
3808381
Box/100
Apex Clipped Corner Slides, White
3800080CL
Case / 1,440
Apex Clipped Corner Slides, Yellow
3800081CL
Case / 1,440
Apex Clipped Corner Slides, Blue
3800082CL
Case / 1,440
Apex Clipped Corner Slides, Green
3800083CL
Case / 1,440
Apex Clipped Corner Slides, Pink
3800084CL
Case / 1,440
Apex Clipped Corner Slides, Lavender
3800085CL
Case / 1,440
Apex Clipped Corner Slides, Orange
3800086CL
Case / 1,440
13
Ariol®
The Complete Microscope-Based, Digital Pathology Platform
Ariol brings added quality, efficiency and confidence to FISH analysis and routine breast cancer IHC tests,
so that patients receive optimal care.
Satisfy increasing laboratory demands by improving efficiency, increasing throughput and lowering sample
turnaround times. Simply put, the Ariol delivers high-quality analysis data sooner.
Barcode-driven workflows can help eliminate bottlenecks and processing errors caused by duplicate data
entry. The batch scanning and analysis capabilities mean the system can function 24/7 allowing routine
operations to be carried out in laboratory downtime, maximizing skilled laboratory workers efficiency.
Ariol features a unique SlideLink workflow that allows the side by side visualization of H&E and FISH slide
images from the same case on one screen. Annotations of key tissue areas by a Pathologist (on the H&E
slide) can be translated to the FISH slide within the system, increasing confidence and allowing
Pathologists input to be gathered off-line. By leveraging Ariol’s SlideLink™ function, laboratories can
revolutionize their FISH analysis and save valuable time spent in the darkroom.
Ariol has the ability to create and customize assays for virtually any tissue FISH based assay for complete
flexibility, future proofing the laboratory for the next generation of personalized medicine assays to enter
routine testing.
Ariol is validated for objective analysis of HER2, ER and PR IHC staining as well as HER2/neu FISH, aiding
in a large number of the diagnostic tests involved in the ever expanding treatment decision tree process.
Ariol is available as an 8 slide position system with an automated stage or as a 200 slide capacity system
with the SL200 autoloader and automated stage.
Intended Use: Ariol Multibay System. ARIOL is an automated scanning microscope and image analysis system. It is intended for In Vitro Diagnostic
use as an aid to the pathologist in the detection, classification and counting of cells of interest based on particular color, intensity, size, pattern and
shape. All diagnostic decisions are made by the qualified clinician.
The Ariol MB8 system stage can accommodate 8 slides
in one run
SlideLink, being used to select areas of capture on a FISH slide from the serial section H&E slide
Automatic cell segmentation of tissue and signal counting in a typical FISH amplification assay
FEATURES
BENEFITS
• Ariol with optional SL200 autoloader increases slide
• Analyze more slides in less time. Analysis with Ariol gives
capacity, enabling unattended slide scanning and FISH
the confidence of objective analysis and reproducible
probe counting. User-defined scanning and analysis
results in breast pathology.
protocols enable batch processing, reducing the need for
manual intervention, increasing system efficiency.
• Extract quantitative data from slides and for ease of use,
express in leading scoring protocols such as HER2 0, 1+,
• Easily compare serial sections of IHC and FISH staining
2+, 3+ or FISH probe ratios.
side-by-side on one screen for improved insight into
expression location. Choose from brightfield, fluorescence • The best image quality by utilizing Leica’s unrivalled
or a combination of both to reflect the tests in your
DM6000 B microscope with advanced automation and
laboratory and maximize usage of your system.
superb image quality.
15
CytoVision®
The Platform for every Cytogenetics and FISH Laboratory
CytoVision is the one image analysis and case management system that provides cytogenetic laboratories with an integrated,
scalable platform for brightfield and fluorescent samples.
Leica Biosystems combines the expertise in image analysis,
The Tissue FISH module brings new levels of automation and
robotics, and microscopy needed to deliver and support a truly
ease of use to FISH analysis. The Tissue FISH module can be
integrated cytogenetics platform.
used on any CytoVision platform from stand-alone FISH
capture stations to high throughput GSL scanning systems with
Used in over 2000 laboratories worldwide, the CytoVision
multiple networked local and remote users.
platform provides the convenience and comfort of on-screen
analysis together with flexibility in software and hardware
FISH scanning can be performed alongside the scanning and
configurations.
analysis of other sample types. CytoVision allows metaphases
for Karyotyping, interphase spot scoring and tissue FISH samples
CytoVision supports all laboratories from the simplest,
to be combined in a single unattended scanning session.
stand-alone FISH capture station to fully networked,
automated, multi-application, nationwide programs.
Hematology and Oncology FISH cases are fully supported by
CytoVision is highly cited in literature across a breadth
CytoVision. Assays for any breakapart, enumeration and fusion
of applications from routine clinical diagnostics to
assays can be quickly created by the user to analyze and report
contemporary stem cell and oncology research.
common Hematology and Oncology panels.
FEATURES
BENEFITS
Scanning, Capture, Analysis and Reporting modules for:
• Bring FISH out of the dark with on screen analysis
• Karyotyping
• Fast and accurate scanning and analysis of Cellular and
• FISH
• Tissue FISH
• Karyotype CGH
Tissue FISH samples
• Scan, manage and analyse FISH cases in parallel
with Karyotypes
• M-FISH
• Reduce turn around times
• Automated/manual spot counting for cellular FISH
• Automate FISH scoring
• Multi-session scoring
• Concurred interpretation of results
• Integrated flexible reporting
• Quickly report results
Intended Use: The Applied Imaging CytoVision™ system is an automated scanning microscope and image analysis system. It is intended for in vitro diagnostic use as
an aid in chromosomal analysis. CytoVision assists in the location of interphase and metaphase nuclei on standard microscope slides using both brightfield and
fluorescent microscopy.
16
GSL Scanning
CytoVision
Data
Internet
CytoVision
Server
LAN
CytoVision Capture Station
CytoVision Review Station
CytoVision offers a scalable network architect where additional scanning, capture and analysis stations can be added. Data is stored in a central database for local review and
analysis or via secure remote access.
Above: CytoVision GSL-120 scanner
Right: CytoVision Probe Case View screen showing
automated scoring and classification results
17
Ordering Information –
Placing Orders
Please contact your local Leica Biosystems sales representative or authorized
dealer, or visit our website LeicaBiosystems.com to locate the local sales
representative for your area.
CONDITIONS OF SALE
PRICING
Products from Leica Biosystems are not intended for drug use,
All prices are exclusive of sale taxes, value added taxes and
nor have they been packaged under sterile conditions.
duties, any other taxes and charges if applicable, and packaging
The listing of any product in this catalog does not imply the
and delivery. While every effort will be made to give reasonable
absence of a patent covering its use, does not constitute
notice of price changes, Leica Biosystems reserves the right to
license under any existing or pending patent, nor is it intended
change prices without notice.
or implied as a recommendation for the use of such products in
infringement of any patent. The responsibility for determining
DELIVERY
the existence of such patents rests solely with the user.
Please contact your local representative for standard
delivery times.
PURCHASING FROM LEICA BIOSYSTEMS
Orders placed with Leica Biosystems must contain the
PAYMENT
following information:
Invoices are payable within 30 days unless otherwise stated
• Delivery address
on the invoice or agreed in writing.
• Invoice address
• Purchase order number
Methods of payment are as follows:
• Name of product, catalog code and quantity
• Check
• Special instructions regarding delivery/packing
• Electronic wire transfer to Leica Biosystems’ bank account,
all charges paid by payee
PURCHASING FROM DISTRIBUTION PARTNERS
Please contact your local Leica Biosystems distribution
partner for terms and conditions.
• Visa or MasterCard where accepted
18
Notes
Leica Biosystems reserves the right to amend specifications without notice. All Rights Reserved. © Leica Biosystems Richmond, Inc. 2014 All third party trademarks are the property of their respective
owners. The Kreatech™ products or the use of the Kreatech™ products is subject to intellectual property rights. FISH and CISH probes are produced under an exclusive license from Veridex LLC for its
REPEAT-FREE™ technology. FISH probes are labeled with the Kreatech’s proprietary Universal Linkage system (ULS™). The following fluorophores (-415; -547;-590;- 647) are subject to patents and know-how,
owned or controlled, and manufactured by DYOMICS GmbH. The ULS™ technology and products are covered by one or more of the following US patents and corresponding patent claims outside the US
owned by KREATECH: US 5,580, 990; 5714,327; 5,985,566; 6,133,038;US RE 40,557E, 6,797,818; 7,217,813.
LeicaBiosystems.com
SLIDE AUTOMATION AND REAGENTS
For a complete solution, Leica Biosystems can assist your
laboratory with a comprehensive range of histology instruments
and matching consumables.
Leica Biosystems brings together
products, quality and support.
Offering a complete solution that helps
you advance workflows, enhance diagnostic
clarity and deliver what really matters –
better patient care.
Leica Biosystems – an international company with a strong network
of worldwide customer service:
Customer and Technical Support
USA
800 248 0123
LEICA BIOSYSTEMS
Leica Biosystems is a global leader in workflow solutions and
automation, striving to advance cancer diagnostics to improve patients’
lives. Leica Biosystems provides anatomical pathology laboratories
and researchers a comprehensive product range for each step in the
pathology process, from sample preparation and staining to imaging and
reporting. Our easy-to-use and consistently reliable offerings help
improve workflow efficiency and diagnostic confidence. The company
is represented in over 100 countries. It has manufacturing facilities
in 7 countries, sales and service organizations in 19 countries, and an
international network of dealers. The company is headquartered in
Nussloch, Germany. Visit LeicaBiosystems.com for more information.
DISCLAIMER
The content of this brochure is intended for the USA. If you are not a
resident in this country please contact your respective sales office to
obtain the appropriate product information for your country of
residence. For more information please visit our website:
LeicaBiosystems.com
95.12457 Rev B ∙ 4/2014 ∙ Copyright © by Leica Biosystems, Richmond, Inc. Illinois, USA,
2014. Subject to modifications. LEICA and the Leica Logo are registered trademarks of
Leica Microsystems IR GmbH.