Training School: Vector-borne diseases and One Health (WG1) Romania, 6-20 July 2015 GOAL AND GENERAL OVERVIEW MAIN ORGANIZERS The theoretical lectures (2 days) will be held in the Facilities of the University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca. David Modrý, UVPS Brno Andrei D. Mihalca, USAMV Cluj-Napoca Lectures will be followed by a series of practical lessons organized within a field trip to a satellite laboratory, in Chilia Veche, Danube Delta Biosphere Reserve. The diversity of agricultural practices and livestock, together with unique and ecologically complex habitats and rural human population represent a exceptional environment in which the One Health paradigm can be practically presented in its broadest meaning, including not only the pathogen transmission between domestic animals and men, but also the importance of wildlife in zoonotic cycles of selected model pathogens. General program will include: sampling and surveillance protocol design, sampling animals, people and environment, laboratory analyses, theoretical approach to data interpretation and application. Such a complex approach is necessary to understand the complex nature of zoonotic vectorborne pathogens and their ecological context. Within European territory, one can hardly imagine more suitable environment. Especially for students from Western Europe, practical work in one of the most valuable parts of remaining European “wilderness” represents a unique chance to understand also the differences in ecological and socio-economic conditions within the expanding territory of EU. The general principles of One Health approach will be exemplified in lectures and practical sessions in field and laboratory using three zoonotic pathogens: Dirofilaria spp. and West Nile Fever virus and their vectors, +Toxoplasma gondii. Maximum attention will be paid to syntheses of obtained data and presentation of the overall aspects underlying the transmission of diseases on human-domestic animalwildlife interface. Training school targets predominantly PhD students and Early Stage Researchers. Training School: Vector-borne diseases and One Health (WG1) Romania, 6-20 July 2015 INVITED TRAINERS KEY ACTIVITIES IN THE FIELD: JAKOB ZINSSTAG • Sampling of hosts (mainly domestic carnivores, ruminants, humans) • collecting the anamnesis data, questionnaires • methods of blood collection from animals under supervision of trained vets • human samples collected in collaboration with health service • laboratory detection (blood smears, Knott test, SNAP tests, serology – ELISA) • Sampling of vectors • ticks • fleas • mosquitoes • dissections of vectors and direct methods of detection of pathogens • Excursions • visits to private farms and households • boat trip though Danube Delta demonstrating all major ecosystems Swiss Tropical and Public Health Institute President, International Association for Ecology & Health THEORETICAL TOPICS: • One Health study designs • Animal-human transmission models • One Health economics PRACTICAL TOPICS: • Human and animal African trypanosomiasis HANS-PETER FÜHRER University of Veterinary Medicine, Vienna FIELD TOPICS: • Mosquitoes: • comparison of trapping methods • taxonomical identification • Serology for viral VDB: • WNV (horses, birds) • Dirofilaria spp. SARA SAVIC Scientific Veterinary Institute "Novi Sad" THEORETICAL TOPICS: • Dirofilariosis in vertebrate hosts FIELD TOPICS: • sampling carnivores and lab. diagnostics DAVID MODRÝ Veterinary and Pharmaceutical University, Brno THEORETICAL AND FIELD TOPICS: • Wildlife and its position within the OH Training School: Vector-borne diseases and One Health (WG1) Romania, 6-20 July 2015 BASIC LOGISTICS • participants are expected to arrive independently to Cluj-Napoca (train, air, car) • registration and accommodation in Cluj-Napoca at dormitory of USAMV • transportation to field station: bus to Tulcea and then by public boat to Chilia-Veche • accommodation, meals and lectures in Chilia-Veche in private decent lodge • laboratory work in basic field lab at Chilia-Veche field station (microscopy, ELISA) • field excursions organized in small groups, walking up to 5 km, 3 FWD cars • three day trip over the Danube Delta Biosphere Reserve: on two rented vessels, lectures onboard • back to Cluj by bus • additional night of accommodation Cluj-Napoca dormitories at USAMV ESTIMATED BUDGET • According to COST rules and TD1303 Internal MC Decisions, each trainee will receive a flat (fixed) grant of 1000 EUR. This is expected to cover all the expenses. • Estimated expenses: - Transportation to/from CJ: 300 EUR - Accommodation + meals in CJ (arrival): 100 EUR - Bus CJ-TL-CJ (+ 3 meals): 70 EUR - Accommodation TL (dinner&BF): 25 EUR - Boat TL-Chilia: 10 EUR - All-inclusive Chilia: 180 EUR - Other expenses in Chilia: 100 EUR - Boat trip (+meals): 200 EUR - Accommodation (inc. BF) CJ (departure): 15 EUR PRELIMINARY SCHEDULE Day 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Date 06-Jul 07-Jul 08-Jul 09-Jul 10-Jul 11-Jul 12-Jul 13-Jul 14-Jul 15-Jul 16-Jul 17-Jul 18-Jul 19-Jul 20-Jul Weekday Mon Tue Wed Thu Fri Sat Sun Mon Tue Wed Thu Fri Sat Sun Mon Activity Arrival (a.m.) Cluj Cluj CJ to TL TL to Chilia Chilia Chilia Chilia Chilia Chilia Boat Boat Boat to TL TL to CJ Departure Sleep Cluj Cluj Cluj Tulcea Chilia Chilia Chilia Chilia Chilia Chilia Field Field Tulcea Cluj
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