Course Specification Suez Canal University Faculty/ Institute: Fish farming and technology Institute 1. Basic Information Code: MBT 401 Title: : Microbial physiology Credit Hours: Lecture: 4 hours Total: 1 hours Level: 4041/ 4042 Practical: / Tutorial: 4 hours 1. Aims of the Course:  To educate students about the different categories of microorganisms including bacteria, viruses & fungi regarding classification, structure, growth characteristics & replication of each microbial category.  To provide students with knowledge about the physiology of microorganisms, the growth curve, and microbial metabolism.  To help student to understand the basic background about microbial genetics and immunology (including innate and acquired immunity). 2. Intended Learning Outcomes (ILOs) a. Knowledge and understanding At the end of this course, student should be able to: b. Intellectual skills a1. Know and describe the classification, morphology, and physiology of different categories of microorganisms (bacteria, viruses & fungi) a4. Discuss main information about bacterial metabolism and bacterial growth a3. Know the basics of microbial genetics. a4. Explain basics of immunology and immunological response At the end of this course, student should be able to: c. Professional and practical skills b1. Differentiate between different microbial categories Distinguish techniques for isolation and identification of microorganisms and their classification C1. Perform different techniques of bacterial staining c2. Examine and identify medically important bacteria based on microscopic examination of stained preparations c3. Isolate and identify pure colonies of microorganisms from different types of specimens using streaking technique C4. Microscopic examination of Fungi & Culture of fungi d- General and transferable skills At the end of this course, student should be able to: d1. Implement writing and presentation skills through creation of a research proposal d2. Communicate clearly by verbal means through group discussions d3. Retrieve and evaluate information from different sources 3. Course Content Week no. Lecture Topic No. of hours 4 Safety measures Week 4 Introduction & Microbial cell components 4 Identification and Classification of bacteria 3 Growth requirements  Temperature  O2 1 Growth requirements  PH  Carbon  Sulfur and others 2 Growth inhibitors 6 Energy generation in bacteria 7 Bacterial Genomics 8 DNA replication 9 Gene transfer in bacteria Practical 4 Media and bacterial isolation 4 Colony characterization & species identification 4 Growth requirements  Temperature 4 Growth requirements  O2 4 Growth requirements  PH 4 Demonstration of growth curve of E. coli 4 Viable count 4 Demonstration of biochemical reactions of E. Coli 4 40 Mutations in bacteria 44 44 43 Protein synthesis Gene expression & regulation Research paper discussion (will be presented by students) Final Exam (in class) 41 4 Demonstration of biochemical reactions of Staphylococcus aureus 4 API system 4 Revision Practical exam 2 2 4. Teaching and learning methods    Lectures Discussions during lectures and tutorials Self-learning through researching, composing. 5. Teaching and learning methods for students with disabilities    Lectures Discussions during lectures. Research and information collection from different sources Self-learning through researching, composing and presenting a scientific proposal.  6. Evaluation  7a. Evaluation methods Formative Evaluation - Quizzes - Assessment  Summative Evaluation - Final Written exams - Final practical exams - Final oral exam Week 6: Midterm exam Week 43: Practical exam Week 41: comprehensive final exam (written and oral) Comprehensive final exam: 10 % Comprehensive oral exam: 40 % Practical exam : 402 Mid term exam: 40 % Research proposal/ oral presentation: 40 % 7b. Exam schedule 7c. Assignment weights 7. Textbooks and references 8a. Course notes Handouts will be given when appropriate 8b. Required textbooks 8c. Recommended reading The book “Microbial Physiology” by Moat, Foster and Spector Www. google.com 8d. Electronic resources Course coordinator: Dr. Samar Solyman 4