Product - HVD LifeScience Vertriebs Ges.m.b.H

Productand Pricelist Stemgent 2015
Tissue Dissociation
ESC/iPSC Cells, Media & Reprogramming Tools
3D Cell Culture Technology & Cell Culture Models
Recombinant Proteins, Cytokines, Biomatrix Proteins & Elisa
Cell Based Assays & Protein Labeling for Microscopy, FACS and HTS
Cell Culture Tools
www.pelobiotech.com
Stemgent Products
2015
Pelobiotech offers you a broad range of products for
ES/iPS Cells from all members of the Reprocell Family
[ReproCell – Stemgent – Reinnervate – BioServe].
Stemgent, Inc. is developing innovative technology
and application solutions for the advancement of
stem cell research. Stemgent products are designed
by leading stem cell researchers worldwide.
Reprogramming Solutions from Stemgent
Through Dr. Shinya Yamanaka’s pioneering work
on the reprogramming of somatic cells to a
pluripotent-like state the iPS cell has become an
invaluable tool for strategies in regenerative
medicine, disease modeling, drug discovery, and
basic research in cell development.
For a whole range of reprogramming methods,
Stemgent provides the quality, knowledge, and
expertise you depend on.
Fig. 1:©Stemgent
Faster, virus-free reprogramming
 mRNA Reprogramming
 microRNA-Enhanced mRNA reprogramming – even faster
mRNA Reprogramming - Generate colonies in as early as 16 days
The optimized Stemgent mRNA Reprogramming System enables non-viral, non-integrating,
clinically-relevant reprogramming of human cells in less than 20 days with an expansion of
new lines in culture in as little as 3 weeks. This is a big step forward, compared to lentivirus,
Sendai virus, and other methods that can take over 20 weeks to confirm viral remnants no
longer remain. Six major steps are required for mRNA reprogramming along with
morphology changes of human iPS cells (as shown in timeline below).
PELOBIOTECH GmbH
Am Klopferspitz 19 | 82152 Planegg | Germany
(P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: [email protected] | www.pelobiotech.com
Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland
Stemgent Products
2015
Fig. 2:Phase-contrast images taken on Day 1-12. Phase-contrast image and fluorescent staining of TRA-1-60 taken on Day
14 (© Stemgent, Inc.)..
microRNA-Enhanced mRNA reprogramming - Generate colonies in as early as
12 days, faster reprogramming kinetics & higher efficiency
microRNAs play an important role in the maintenance and retention of pluripotency for both ES cells
and iPS cells, as ongoing research has demonstrated 1,2. These endogenous, small, non-coding RNAs
target and down-regulate mRNAs by blocking gene expression via translational repression or
degradation of functionally transcribed mRNAs. In 2012, two groups succeeded in functionally
reprogramming human fibroblasts to a pluripotent state in the absence of exogenous
reprogramming factor expression, but with the help of specific microRNA combinations 3, 4, 5.
microRNAs induce self-renewal and proliferative networks, inhibit TLR-induced inflammatory
cytokine expression, and promote a mesenchymal to epithelial (MET) transition, which is particularly
advantageous when working with difficult to reprogram patient samples 6, 7.
Stemgent developed a new microRNA-enhanced mRNA Reprogramming method, which combines
the use of the Stemgent mRNA Reprogramming Kit, the Stemgent microRNA Booster Kit, and the
StemfectTM RNA Transfection Kit, to generate high-quality human iPS cell lines. These modifications
to the traditional feeder-based mRNA reprogramming protocol results in faster reprogramming
kinetics (see timeline Fig. 3), simpler experimental set up simpler, allows for reprogramming more
than twice the number of wells (9 wells in 6-well format) and shows higher efficiency. This method is
recommended for achieving the best results from your reprogramming experiment when compared
to other available reprogramming methods.
Fig. 3: microRNA enhanced mRNA Reprogramming Timeline. 2 microRNA transfections and 11 mRNA transfections in total
are required for this system. By Day 13, emerging iPS cell colonies are often able to be identified by morphology and life
staining. But depending on the target cell type, cell colonies may be ready to be picked and replated earlier or later than
shown in the timeline (© Stemgent, Inc..).
PELOBIOTECH GmbH
Am Klopferspitz 19 | 82152 Planegg | Germany
(P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: [email protected] | www.pelobiotech.com
Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland
Stemgent Products
2015
Fig. 4: Morphological progression comparison of diseased patient fibroblasts during reprogramming with and without
microRNA: The above panel demonstrates representative morphological progression of adult diseased patient fibroblasts
with and without the Stemgent-Asterand microRNA booster cocktail (© Stemgent, Inc..).
1. Takhashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., Yamanaka S. (2007) Induction of
pluripotent stem cells from adult human fibroblasts by defined factors. Cell, 131 (5): 861 - 872.
2. Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A.,
Ruotti, V., Stewart, R., Slukvin, I.I., Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human
somatic cells. Science, 318 (5858): 1917 – 1920.
3. Mallanna, S. and Rizzino, A. (2010) Emerging roles of microRNAs in the control of embryonic stem cells and
the generation of induced pluripotent stem cells. Dev Biol. 344: 16-25.
4. Anokye-Danso, F., Trivedi, C. M., Juhr, D., Gupta, M., Cui, Z., Tian, Y., Zhang, Y., Yang, W., Gruber, P. J.,
Epstein, J. A., and Morrisey, E. E. (2011) Highly Efficient miRNA-Mediatd Reprogramming of Mouse and Human
Somatic Cells to Pluripotency. Cell Stem Cell. 8 (4): 376 – 388.
5. Miyoshi, N., Ishii, H., Nagano, H., Haraguchi, N., Dewi, D. L., Kano, Y., Nishikawa, S., Tanemura, M., Mimori,
K., Tanaka, F., Saito, T., Nishimura, J., Takemasa, I., Mizushima, T., Ikeda, M., Yamamoto. H., Sekimoto, M.,
Doki, Y., and Mori, M. (2011) Reprogramming of Mouse and Human Cells to Pluripotency Using Mature
MicroRNAs. Cell Stem Cell 8: 633 – 638.
6. Liao, B., Bao, X., Liu, L., Feng, S., Zovoilis, Z., Liu, W., Xue, Y., Cai, J., Guo, X., Qin, B., Zhang, R., Wu, J., Lai,
L., Teng, M., Niu, L., Zhang, B., Esteban, M. A., Pei, D. (2011) MicroRNA Cluster 302–367 Enhances Somatic
Cell Reprogramming by Accelerating a Mesenchymal-to-Epithelial Transition. The Journal of Biological Chemistry,
286, 17359 – 17364.
7. Kuo, C-H., Ying, S-Y. (2013) MicroRNA-Mediated Somatic Cell Reprogramming. Journal of Cellular
Biochemistry, 114:275 – 281.
PELOBIOTECH GmbH
Am Klopferspitz 19 | 82152 Planegg | Germany
(P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: [email protected] | www.pelobiotech.com
Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland
Stemgent Products
2015
Products for Reprogramming
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




Reprogramming Systems (13)
Transfection (2)
Antibodies (19)
Cell Lines (4)
Media (3)
Cytokines / Proteins (1)
Small Molecules (5)
Catalog #
Brand
Product
Size
Reprogramming Tools
Reprogramming Systems
05-0018
05-0020
05-0015
05-0022
05-0017
00-0073
00-0067
Stemgent®
Stemgent®
Stemgent®
Stemgent®
Stemgent®
Stemgent®
Stemgent®
c-Myc mRNA, Human
eGFP mRNA
Klf4 mRNA, Human
L-Myc mRNA, Human
Lin28 mRNA, Human
microRNA Booster Kit
mRNA Reprogramming Factors
Set: hOKSML
20 μg
20 μg
20 μg
20 μg
20 μg
kit
1 Set
00-0071
Stemgent®
mRNA Reprogramming Kit
1 kit
00-0071mi
Stemgent®
microRNA-Enhanced mRNA
Reprogramming Kit
1 kit
05-0021
05-0019
05-0014
05-0016
00-0068
Stemgent®
Stemgent®
Stemgent®
Stemgent®
Stemgent®
Nanog mRNA, Human
nGFP mRNA
Oct4 mRNA, Human
Sox2 mRNA, Human
VSVg Retrovirus Reprogramming
Set: Human OKSM
20 μg
20 μg
20 μg
20 μg
1 set
Transfection
05-0020
00-0069
Stemgent®
Stemfect™
eGFP mRNA
RNA Transfection Kit
20 μg
1 Kit
Antibodies
00-0009
Stemgent®
Alkaline Phosphatase Staining Kit
50 assays
PELOBIOTECH GmbH
Am Klopferspitz 19 | 82152 Planegg | Germany
(P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: [email protected] | www.pelobiotech.com
Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland
Stemgent Products
2015
Catalog #
00-0055
Brand
Stemgent®
Product
Alkaline Phosphatase Staining Kit II
Size
50 assays
09-0021
Stemgent®
Klf4 Antibody (Purified), Mouse
anti-Mouse/Human
100 μl
09-0020
Stemgent®
Nanog Antibody (Affinity Purified),
Rabbit anti-Mouse/Human
100 μl
09-0023
Stemgent®
Oct4 Antibody (Affinity Purified),
Rabbit anti-Mouse/Human
100 μl
09-0024
Stemgent®
Sox2 Antibody (Affinity Purified),
Rabbit anti-Mouse/Human
100 μl
09-0067
StainAlive™
SSEA-1 Antibody (DyLight™ 488),
Mouse anti-Mouse/Human
100 μl
09-0095
StainAlive™
SSEA-1 Antibody (DyLight™ 550),
Mouse anti-Mouse/Human
100 μl
09-0001
Stemgent®
SSEA-1 Antibody (PE), Mouse antiMouse/Human
1 ml
09-0005
Stemgent®
SSEA-1 Antibody (Purified), Mouse
anti-Mouse/Human
100 μl
09-0044
Stemgent®
SSEA-3 Antibody (PE), Rat antiHuman
1 ml
09-0014
Stemgent®
SSEA-3 Antibody (Purified), Rat
anti-Mouse/Human
100 μl
09-0003
Stemgent®
SSEA-4 Antibody (PE), Mouse antiHuman
1 ml
09-0006
Stemgent®
SSEA-4 Antibody (Purified), Mouse
anti-Human
100 μl
PELOBIOTECH GmbH
Am Klopferspitz 19 | 82152 Planegg | Germany
(P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: [email protected] | www.pelobiotech.com
Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland
Stemgent Products
2015
Catalog #
09-0068
Brand
StainAlive™
Product
TRA-1-60 Antibody (DyLight™
488), Mouse anti-Human
Size
09-0009
Stemgent®
TRA-1-60 Antibody (PE), Mouse
anti-Human
1 ml
09-0010
Stemgent®
TRA-1-60 Antibody (Purified),
Mouse anti-Human
100 μl
09-0069
StainAlive™
TRA-1-81 Antibody (DyLight™
488), Mouse anti-Human
100 μl
09-0011
Stemgent®
TRA-1-81 Antibody (Purified),
Mouse anti-Human
100 μl
100 μl
Cell Lines
08-0006
Stemgent®
Mouse Primary iPS Cells-T1b
2 x 105 cells/vial
08-0007
Stemgent®
Mouse Primary iPS Cells-WP5
2 x 105 cells/vial
GSC-3002G
Stemgent®
Stemgent® NuFF-RQ™ IRR
4-5 x 106 cells/vial
GSC-3006G
Stemgent®
Stemgent® NuFF-RQ™ IRR
4-5 x 106 cells/vial
Media
01-0014-500
Stemgent®
hES Cell Cloning & Recovery
Supplement
5 x 100 μl
00-0070
Pluriton™
Reprogramming Medium
500 ml
01-0005
01-0005-100
NutriStem™
XF/FF Culture Medium
500 ml
100 ml
Cytokines / Proteins
03-0002
Stemfactor™
FGF-basic, Human Recombinant
50 μg
Small Molecules
04-0016
04-0017
04-0007
04-0012
04-0012-10
04-0012-02
Stemolecule™
Stemolecule™
Stemolecule™
Stemolecule™
Doxycycline hyclate
Thiazovivin
Valproic Acid
Y27632
Stemolecule™
Y27632 in Solution
10 mg
1 mg
5g
2 mg
10 mg
2 mg (10 mM)
PELOBIOTECH GmbH
Am Klopferspitz 19 | 82152 Planegg | Germany
(P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: [email protected] | www.pelobiotech.com
Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland
Stemgent Products
2015
Self-renewal Solutions from Stemgent
The key feature of ES and iPS cells, that distinguishes them from all other cells, is the ability
to proliferate indefinitely while maintaining the capacity to differentiate into many cell
types. Therefore ES and iPS cells present an enormous potential as biological tools in
strategies for regenerative medicine, drug discovery, disease models, as well as for research
in developmental biology. However, physical and chemical stimuli from the environment
influence these cells’ behavior by triggering signaling pathways that directs them into
remaining a pluripotent state or differentiating into a mature cell type. This sensitivity of
pluripotent stem cells towards environmental changes has proven to be a major challenge.
A number of factors that should be considered for sustained culturing of ES and iPS stem
cells include:
1. Defined cell culture medium for reproducibility
2. Defined media components at optimal concentrations; cytokines (growth factors)
and small molecules
3. Optimal cell density
4. Methods for passaging cells that do not force differentiation
5. Defined basement membrane substrates that facilitate cell attachment
ES and iPS cell cultures should be routinely monitored for pluripotency, because of their
extreme sensitivity,.
Some of the key assays include:
1. Normal colony morphology and rate of proliferation
2. Expression of key pluripotency genes and absence of lineage-specific genes (for iPS
cells)
3. Expression of key pluripotency surface markers
4. Ability to differentiate into cells from all three primary germline layer tissues in vivo
and in vitro
5. Normal genetic karyotype
New technologies for stem cell research are being developed with increasing speed. Our
teams at Stemgent work diligently with our Scientific Advisory Board and other leading stem
cell scientists to validate and deliver cutting-edge reagents that enable you to more readily
culture and characterize ES and iPS cells.
Products for Self-Renewal
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Antibodies (19)
Media (3)
Cytokines / Proteins (4)
PELOBIOTECH GmbH
Am Klopferspitz 19 | 82152 Planegg | Germany
(P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: [email protected] | www.pelobiotech.com
Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland
Stemgent Products
2015

Small Molecules (12)
Catalog #
Brand
Product
Size
Self-Renewal
Antibodies
00-0009
temgent®
Alkaline Phosphatase Staining Kit
50 assays
00-0055
Stemgent®
Alkaline Phosphatase Staining Kit II
50 assays
09-0021
Stemgent®
Klf4 Antibody (Purified), Mouse
anti-Mouse/Human
100 μl
09-0020
Stemgent®
Nanog Antibody (Affinity Purified),
Rabbit anti-Mouse/Human
100 μl
09-0023
Stemgent®
Oct4 Antibody (Affinity Purified),
Rabbit anti-Mouse/Human
100 μl
09-0024
Stemgent®
Sox2 Antibody (Affinity Purified),
Rabbit anti-Mouse/Human
100 μl
09-0067
StainAlive™
SSEA-1 Antibody (DyLight™ 488),
Mouse anti-Mouse/Human
100 μl
09-0095
StainAlive™
SSEA-1 Antibody (DyLight™ 550),
Mouse anti-Mouse/Human
100 μl
09-0001
Stemgent®
SSEA-1 Antibody (PE), Mouse antiMouse/Human
1 ml
09-0005
Stemgent®
SSEA-1 Antibody (Purified), Mouse
anti-Mouse/Human
100 μl
09-0044
Stemgent®
SSEA-3 Antibody (PE), Rat antiHuman
1 ml
PELOBIOTECH GmbH
Am Klopferspitz 19 | 82152 Planegg | Germany
(P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: [email protected] | www.pelobiotech.com
Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland
Stemgent Products
2015
Catalog #
09-0014
Brand
Stemgent®
Product
SSEA-3 Antibody (Purified), Rat
anti-Mouse/Human
Size
09-0003
Stemgent®
SSEA-4 Antibody (PE), Mouse antiHuman
1 ml
09-0006
Stemgent®
SSEA-4 Antibody (Purified), Mouse
anti-Human
100 μl
09-0068
StainAlive™
TRA-1-60 Antibody (DyLight™
488), Mouse anti-Human
100 μl
09-0009
Stemgent®
TRA-1-60 Antibody (PE), Mouse
anti-Human
1 ml
09-0010
Stemgent®
TRA-1-60 Antibody (Purified),
Mouse anti-Human
100 μl
09-0069
StainAlive™
TRA-1-81 Antibody (DyLight™
488), Mouse anti-Human
100 μl
09-0011
Stemgent®
TRA-1-81 Antibody (Purified),
Mouse anti-Human
100 μl
100 μl
Media
01-0013-50
01-0014-500
CryoStem™
Stemgent®
Freezing Medium
hES Cell Cloning & Recovery
Supplement
50 ml
5 x 100 μl
01-0005
01-0005-100
NutriStem™
XF/FF Culture Medium
500 ml
100 ml
Cytokines / Proteins
03-0001
Stemfactor™
Activin A, Human Recombinant
5 μg
03-0007
Stemfactor™
BMP-4, Human Recombinant
10 μg
03-0002
Stemfactor™
FGF-basic, Human Recombinant
50 μg
03-0004
Stemfactor™
TGF-β1, Human Recombinant
5 μg
Small Molecules
04-0003
04-0004
Stemolecule™
Stemolecule™
BIO
CHIR99021
2 mg
2 mg
PELOBIOTECH GmbH
Am Klopferspitz 19 | 82152 Planegg | Germany
(P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: [email protected] | www.pelobiotech.com
Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland
Stemgent Products
2015
Catalog #
04-0004-10
04-0004-02
04-0049
04-0006
04-0006-10
04-0006-02
04-0008
04-0030
04-0011
04-0017
04-0012
04-0012-10
04-0012-02
Brand
Product
Stemolecule™
Stemolecule™
Stemolecule™
CHIR99021 in Solution
GSK429286A
PD0325901
Stemolecule™
Stemolecule™
Stemolecule™
Stemolecule™
Stemolecule™
Stemolecule™
PD0325901 in Solution
PD173074
ROCK II Inhibitor
SC1 (Pluripotin)
Thiazovivin
Y27632
Stemolecule™
Y27632 in Solution
Size
10 mg
2 mg (10 mM)
2 mg
2 mg
10 mg
2 mg (10 mM)
2 mg
2 mg
1 mg
1 mg
2 mg
10 mg
2 mg (10 mM)
Differentiaton Solutions from Stemgent
The stem cell community has endeavored to find culture conditions and key signaling factors
to guide stem cell differentiation towards clinically relevant cell fates, since the derivation of
human embryonic stem cells in 1981. In vitro differentiation cell culture strategies were
designed from signaling cues gained from in vivo development.
Novel small molecules were discovered thanks to recent advances in high-throughput
robotics and chemical synthesis. These molecules can be used alone or with more traditional
cell culture reagents such as cytokines and extracellular matrix (ECM) substrates. We
recommend to test the best ECM conditions first (MicroMatrix Arrays) as the
microenvironment has a huge influence on stem cell differentiation. Stemgent offers
consistent robust reagents for stem cell differentiation towards:
1. Ectoderm: with cytokines (e.g. SHH, Noggin), small molecules (e.g. SHH Signaling
Pathway Modulator Set); and matrices (Laminin).
2. Endoderm: with cytokines (e.g. Activin A) and small molecules (e.g. IDE-1, IDE-2).
3. Mesoderm: with cytokines (e.g. BMP-4, Wnt-3a) and small molecules (e.g. Wnt
Signaling Pathway Set)
Inspired by the success of reprogramming mature cells to iPS cells, Marius Wernig2 and
Douglas Melton3 have lead the way to identifying key transcription factors that directly
differentiate skin cells to neurons (Ascl1, Brn2, and Myt1l) and pancreatic exocrine cells into
insulin-producing beta-cells (Ngn3, Pdx1, and Mafa). Stemgent newly offers viral vectors for
the directed differentiation of one mature cell type to another mature cell type, which
reduces the experimental time between patient tissue collection and derivation of cell type
of interest in regenerative medicine.
PELOBIOTECH GmbH
Am Klopferspitz 19 | 82152 Planegg | Germany
(P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: [email protected] | www.pelobiotech.com
Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland
Stemgent Products
2015
Our teams at Stemgent work diligently with our Scientific Advisory Board and other leading
stem cell scientists to validate and deliver cutting-edge reagents that enable you to more
readily generate, differentiate, culture, and characterize ES/iPS cells for use in strategies in
regenerative medicine, disease modeling, drug discovery, and developmental biology.
References
1. Thomson J.A., Itskovitz-Eldor J., Shapiro S.S., Waknitz M.A., Swiergiel J.J., Marshall V.S., Jones
J.M. (1998) Embryonic Stem Cell Lines Derived from Human Blastocysts. Science, 282:1145–
1147.
2. Vierbuchen T., Ostermeier A., Pang Z.P., Kokubu Y., Südhof T.C., Wernig M. (2010) Direct
conversion of fibroblasts to functional neurons by defined factors. Nature, 463(7284):103541.
3. Zhou Q., Brown J., Kanarek A., Rajagopal J., Melton D.A. (2008) In vivo reprogramming of
adult pancreatic exocrine cells to beta-cells. Nature, 455(7213):627-32.
Products for Differentiation
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

Antibodies (1)
Media (3)
Cytokines / Proteins (6)
Small Molecules (29)
Differentiation (7)
Catalog #
Brand
Product
Size
Differentiation
Antibodies
09-0075
Stemgent®
Pax6 Antibody (Purified), Rabbit
anti-Mouse/Human
100 μl
Media
01-0014-500
Stemgent®
hES Cell Cloning & Recovery
Supplement
5 x 100 μl
00-0072
Stemgent®
MesoFate™ Differentiation
Medium
500 ml +
supplement
01-0005
01-0005-100
NutriStem™
XF/FF Culture Medium
500 ml
100 ml
Cytokines / Proteins
03-0001
Stemfactor™
Activin A, Human Recombinant
5 μg
03-0007
Stemfactor™
BMP-4, Human Recombinant
10 μg
PELOBIOTECH GmbH
Am Klopferspitz 19 | 82152 Planegg | Germany
(P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: [email protected] | www.pelobiotech.com
Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland
Stemgent Products
2015
Catalog #
03-0002
Brand
Stemfactor™
Product
FGF-basic, Human Recombinant
Size
50 μg
03-0006
Stemfactor™
Noggin, Human Recombinant
10 μg
03-0008
Stemfactor™
Sonic Hedgehog, Human
Recombinant
25 μg
03-0004
Stemfactor™
TGF-β1, Human Recombinant
5 μg
Small Molecules
04-0052
04-0014
04-0014-10
04-0015
04-0021
04-0051
04-0003
04-0004
04-0004-10
04-0004-02
04-0022
04-0041
04-0024
04-0025
04-0026
04-0027
04-0037
04-0028
04-0050
04-0074
04-0074-10
04-0074-02
Stemolecule™
Stemolecule™
A769662
A83-01
Stemolecule™
Stemolecule™
Stemolecule™
Stemolecule™
Stemolecule™
ALK5 Inhibitor
All-Trans Retinoic Acid
BI-D1870
BIO
CHIR99021
Stemolecule™
Stemolecule™
Stemolecule™
Stemolecule™
Stemolecule™
Stemolecule™
Stemolecule™
Stemolecule™
Stemolecule™
Stemolecule™
Stemolecule™
CHIR99021 in Solution
Cyclopamine
DAPT
Dorsomorphin
Forskolin
IDE-1
IDE-2
IPA-3
KAAD-Cyclopamine
Ku-0063794
LDN-193189
Stemolecule™
LDN-193189 in Solution
04-0008
04-0009
04-0010
04-0010-10
04-0010-05
04-0032
04-0031
04-0034
Stemolecule™
Stemolecule™
Stemolecule™
PD173074
Purmorphamine
SB431542
Stemolecule™
Stemolecule™
Stemolecule™
Stemolecule™
SB431542 in Solution
SHH Antagonist
SMO Antagonist
Wnt Inhibitor IWP-2
10 mg
2 mg
10 mg
1 mg
100 mg
10 mg
2 mg
2 mg
10 mg
2 mg (10 mM)
2 mg
5 mg
2 mg
10 mg
2 mg
2 mg
5 mg
100 μg
2 mg
2 mg
10 mg
2 mg (10 mM)
2 mg
5 mg
5 mg
10 mg
5 mg (10 mM)
2 mg
2 mg
2 mg
PELOBIOTECH GmbH
Am Klopferspitz 19 | 82152 Planegg | Germany
(P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: [email protected] | www.pelobiotech.com
Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland
Stemgent Products
2015
Catalog #
04-0035
04-0036
04-0046
Brand
Stemolecule™
Stemolecule™
Stemolecule™
Product
Wnt Inhibitor IWP-3
Wnt Inhibitor IWP-4
XAV939
Size
2 mg
2 mg
2 mg
mRNA´s for Differentiation
05-0033
05-0034
00-0072
Stemgent®
Stemgent®
Stemgent®
Gata4 mRNA, Human
Mef2c mRNA, Human
MesoFate™ Differentiation
Medium
20 µg
20 µg
500 ml +
supplement
05-0031
05-0029
05-0032
05-0035
Stemgent®
Stemgent®
Stemgent®
Stemgent®
Myf5 mRNA, Human
MyoD mRNA, Human
Myog mRNA, Human
Tbx5 mRNA, Human
20 µg
20 µg
20 µg
20 µg
PELOBIOTECH GmbH
Am Klopferspitz 19 | 82152 Planegg | Germany
(P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: [email protected] | www.pelobiotech.com
Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland
Stemgent Prices 2015
sorted by Cat#
Cat#
Product
Quantity
00-0009
00-0047-500
00-0051-500
00-0052-500
00-0055
00-0067
Stemgent® Alkaline Phosphatase Staining Kit
WIT-T™ Culture Medium
WIT-P-NC™ Culture Medium
WIT-I-NC™ Culture Medium
Stemgent® Alkaline Phosphatase Staining Kit II
Stemgent® mRNA Reprogramming Factors Set:
hOKSML
Stemgent® VSVg Retrovirus Reprogramming Set:
Human OKSM
Stemfect™ RNA Transfection Kit
Pluriton™ Reprogramming Medium
Stemgent® mRNA Reprogramming Kit
Stemgent® MesoFate™ Differentiation Media
Stemgent®microRNA Booster Kit
Renaissance™Essential Tumor Medium
NutriStem® XF/FF Culture Medium
NutriStem® XF/FF Culture Medium
CryoStem™ Freezing Medium
Stemgent® hES Cell Cloning & Recovery Supplement
Stemfactor™ Activin A, Human Recombinant
Stemfactor™ FGF-basic, Human Recombinant
Stemfactor™ TGF-β1, Human Recombinant
Stemfactor™ Noggin, Human Recombinant
Stemfactor™ BMP-4, Human Recombinant
Stemfactor™ Sonic Hedgehog, Human Recombinant
Stemfactor™ LIF, Mouse Recombinant
Stemfactor™ LIF, Mouse Recombinant
Stemfactor™ LIF, Human Recombinant
Stemfactor™ LIF, Human Recombinant
Stemolecule™ RG108
Stemolecule™ BIX01294
Stemolecule™ BIO
Stemolecule™ CHIR99021
Stemolecule™ CHIR99021 in Solution
Stemolecule™ CHIR99021
Stemolecule™ Sodium Butyrate
Stemolecule™ PD0325901
Stemolecule™ PD0325901 in Solution
Stemolecule™ PD0325901
Stemolecule™ Valproic Acid
Stemolecule™ PD173074
Stemolecule™ Purmorphamine
Stemolecule™ SB431542
Stemolecule™ SB431542 in Solution
Stemolecule™ SB431542
Stemolecule™ SC1 (Pluripotin)
Stemolecule™ Y27632
50 assays
500 ml
500 ml
500 ml
50 assays
1 Set
00-0068
00-0069
00-0070
00-0071
00-0072
00-0073
00-0074
01-0005
01-0005-100
01-0013-50
01-0014-500
03-0001
03-0002
03-0004
03-0006
03-0007
03-0008
03-0011
03-0011-100
03-0016
03-0016-100
04-0001
04-0002
04-0003
04-0004
04-0004-02
04-0004-10
04-0005
04-0006
04-0006-02
04-0006-10
04-0007
04-0008
04-0009
04-0010
04-0010-05
04-0010-10
04-0011
04-0012
1 set
1 Kit
500 ml
1 kit
500 ml
1 kit
500 ml
500 ml
100 ml
50 ml
5 x 100 μl
5 μg
50 μg
5 μg
10 μg
10 μg
25 μg
1 ml, 10 μg/ml
1 ml, 100 μg/ml
1 ml, 10 μg/ml
1 ml, 100 μg/ml
10 mg
5 mg
2 mg
2 mg
2 mg (10 mM)
10 mg
500 mg
2 mg
2 mg (10 mM)
10 mg
5g
2 mg
5 mg
5 mg
5 mg (10 mM)
10 mg
1 mg
2 mg
PELOBIOTECH GmbH
Am Klopferspitz 19 | 82152 Planegg | Germany
(P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: [email protected] | www.pelobiotech.com
Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland
Price
(EUR)
75,00
226,00
226,00
226,00
86,00
1348,00
644,00
247,00
202,00
1796,00
238,00
627,00
238,00
211,00
65,00
106,00
142,00
146,00
117,00
198,00
122,00
201,00
261,00
211,00
872,00
231,00
1176,00
126,00
98,00
98,00
187,00
219,00
805,00
51,00
187,00
219,00
805,00
51,00
117,00
98,00
94,00
94,00
155,00
98,00
117,00
Stemgent Prices 2015
Cat#
Product
Quantity
04-0012-02
04-0012-10
04-0013
04-0014
04-0014-10
04-0015
04-0016
04-0017
04-0021
04-0022
04-0024
04-0025
04-0026
04-0027
04-0028
04-0030
04-0031
04-0032
04-0033
04-0034
04-0035
04-0036
04-0037
04-0039
04-0041
04-0044
04-0046
04-0047
04-0049
04-0050
04-0051
04-0052
04-0054
04-0072
04-0074
04-0074-02
04-0074-10
04-0076
04-0077
04-0078
05-0014
05-0015
05-0016
05-0017
05-0018
05-0019
05-0020
Stemolecule™ Y27632 in Solution
Stemolecule™ Y27632
Stemolecule™ R(+)Bay K 8644
Stemolecule™ A83-01
Stemolecule™ A83-01
Stemolecule™ ALK5 Inhibitor
Stemolecule™ Doxycycline hyclate
Stemolecule™ Thiazovivin
Stemolecule™ All-Trans Retinoic Acid
Stemolecule™ Cyclopamine
Stemolecule™ Dorsomorphin
Stemolecule™ Forskolin
Stemolecule™ IDE-1
Stemolecule™ IDE-2
Stemolecule™ KAAD-Cyclopamine
Stemolecule™ ROCK II Inhibitor
Stemolecule™ SMO Antagonist
Stemolecule™ SHH Antagonist
Stemolecule™ Tranylcypromine hydrochloride
Stemolecule™ Wnt Inhibitor IWP-2
Stemolecule™ Wnt Inhibitor IWP-3
Stemolecule™ Wnt Inhibitor IWP-4
Stemolecule™ IPA-3
Stemolecule™ Pifithrin-μ
Stemolecule™ DAPT
Stemolecule™ KP372-1
Stemolecule™ XAV939
Stemolecule™ GDC-0941
Stemolecule™ GSK429286A
Stemolecule™ Ku-0063794
Stemolecule™ BI-D1870
Stemolecule™ A769662
Stemolecule™ LY411575
Stemolecule™ Pyrintegrin
Stemolecule™ LDN-193189
Stemolecule™ LDN-193189 in Solution
Stemolecule™ LDN-193189
Stemolecule™ PS48
Stemolecule™ AM580
Stemolecule™ CD437
Stemgent® Oct4 mRNA, Human
Stemgent® Klf4 mRNA, Human
Stemgent® Sox2 mRNA, Human
Stemgent® Lin28 mRNA, Human
Stemgent® c-Myc mRNA, Human
Stemgent® nGFP mRNA
Stemgent® eGFP mRNA
2 mg (10 mM)
10 mg
5 mg
2 mg
10 mg
1 mg
10 mg
1 mg
100 mg
2 mg
2 mg
10 mg
2 mg
2 mg
100 μg
2 mg
2 mg
2 mg
10 mg
2 mg
2 mg
2 mg
5 mg
5 mg
5 mg
5 mg
2 mg
5 mg
2 mg
2 mg
10 mg
10 mg
5 mg
1 mg
2 mg
2 mg (10 mM)
10 mg
5 mg
5 mg
5 mg
20 μg
20 μg
20 μg
20 μg
20 μg
20 μg
20 μg
PELOBIOTECH GmbH
Am Klopferspitz 19 | 82152 Planegg | Germany
(P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: [email protected] | www.pelobiotech.com
Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland
Price
(EUR)
129,00
517,00
145,00
112,00
413,00
98,00
51,00
237,00
88,00
155,00
107,00
145,00
230,00
230,00
239,00
230,00
230,00
230,00
51,00
98,00
98,00
98,00
98,00
79,00
136,00
309,00
230,00
173,00
239,00
173,00
497,00
211,00
230,00
249,00
187,00
219,00
805,00
79,00
111,00
129,00
229,00
229,00
229,00
229,00
229,00
229,00
229,00
Stemgent Prices 2015
Cat#
Product
Quantity
05-0021
05-0022
05-0029
05-0031
05-0032
05-0033
05-0034
05-0035
08-0006
Stemgent® Nanog mRNA, Human
Stemgent® L-Myc mRNA, Human
Stemgent® MyoD mRNA, Human
Stemgent® Myf5 mRNA, Human
Stemgent® Myog mRNA, Human
Stemgent® Gata4 mRNA, Human
Stemgent® Mef2c mRNA, Human
Stemgent® Tbx5 mRNA, Human
Stemgent® Mouse Primary iPS Cells-T1b
08-0007
Stemgent® Mouse Primary iPS Cells-WP5
09-0001
Stemgent® SSEA-1 Antibody (PE), Mouse antiMouse/Human
Stemgent® SSEA-4 Antibody (PE), Mouse antiHuman
Stemgent® SSEA-1 Antibody (Purified), Mouse antiMouse/Human
Stemgent® SSEA-4 Antibody (Purified), Mouse antiHuman
Stemgent® TRA-1-60 Antibody (PE), Mouse antiHuman
Stemgent® TRA-1-60 Antibody (Purified), Mouse
anti-Human
Stemgent® TRA-1-81 Antibody (Purified), Mouse
anti-Human
Stemgent® SSEA-3 Antibody (Purified), Rat antiMouse/Human
Stemgent® Nanog Antibody (Affinity Purified),
Rabbit anti-Mouse/Human
Stemgent® Klf4 Antibody (Purified), Mouse antiMouse/Human
Stemgent® Oct4 Antibody (Affinity Purified), Rabbit
anti-Mouse/Human
Stemgent® Sox2 Antibody (Affinity Purified), Rabbit
anti-Mouse/Human
Stemgent® Goat anti-Rabbit IgG Antibody (DyLight™
488)
Stemgent® SSEA-3 Antibody (PE), Rat anti-Human
StainAlive™ SSEA-1 Antibody (DyLight™ 488), Mouse
anti-Mouse/Human
StainAlive™ TRA-1-60 Antibody (DyLight™ 488),
Mouse anti-Human
StainAlive™ TRA-1-81 Antibody (DyLight™ 488),
Mouse anti-Human
StainAlive™ Mouse IgM, κ Isotype Control (DyLight™
488)
20 μg
20 μg
20 µg
20 µg
20 µg
20 µg
20 µg
20 µg
2 x 105
cells/vial
2 x 105
cells/vial
1 ml
09-0003
09-0005
09-0006
09-0009
09-0010
09-0011
09-0014
09-0020
09-0021
09-0023
09-0024
09-0034
09-0044
09-0067
09-0068
09-0069
09-0072
Price
(EUR)
229,00
229,00
229,00
229,00
229,00
229,00
229,00
229,00
474,00
474,00
151,00
1 ml
151,00
100 μl
170,00
100 μl
161,00
1 ml
133,00
100 μl
186,00
100 μl
96,00
100 μl
133,00
100 μl
244,00
100 μl
133,00
100 μl
170,00
100 μl
157,00
200 μl
80,00
1 ml
100 μl
142,00
238,00
100 μl
238,00
100 μl
238,00
100 μl
97,00
PELOBIOTECH GmbH
Am Klopferspitz 19 | 82152 Planegg | Germany
(P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: [email protected] | www.pelobiotech.com
Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland
Stemgent Prices 2015
Cat#
Product
Quantity
09-0075
Stemgent® Pax6 Antibody (Purified), Rabbit antiMouse/Human
StainAlive™ SSEA-1 Antibody (DyLight™ 550), Mouse
anti-Mouse/Human
StainAlive™ SSEA-4 Antibody (DyLight™ 550), Mouse
anti-Human
100 μl
Price
(EUR)
244,00
100 μl
238,00
100 μl
238,00
09-0095
09-0097
Contact in Austria
our partner HVD Life Science Vertriebs GmbH
Wurzbachgasse 18
1152 Wien
(P) +43 1 98 295 26 30 | (F) +43 1 786 36 44
Email: [email protected]
Terms and Conditions / Lieferbedingungen
Prices are valid from February 1st, 2015. All prices are net prices excluding tax and any applicable
duties. All prices are subject to change without prior notice. Terms of delivery: EXW, Martinsried
Germany. Errors and misprints are excepted.
Die Preise sind gültig ab dem 1. Februar 2015.
Preise: zzgl. MwSt.;
Lieferung: ab Werk Martinsried, Deutschland , zuzüglich Transport- und Kühlgebühren
Alle Preise können ohne Ankündigung geändert werden. Irrtümer und Druckfehler vorbehalten.
All products are for research use only. Not for use in humans!
PELOBIOTECH GmbH
Am Klopferspitz 19 | 82152 Planegg | Germany
(P) +49 (0)89 517 286 59-0 | (F) +49 (0)89 517 286-88 |E-Mail: [email protected] | www.pelobiotech.com
Pelobiotech distributes all Stemgent products in Austria, Germany and Switzerland