Benzodiazepines
Homogeneous Enzyme Immunoassay (HEIA™)
Benzodiazepines are among the most commonly prescribed
medications in the United States, being used in the treatment
Target Analyte: Oxazepam (Serax®)
of anxiety, pain relief, sedation and as anti-convulsants.
Although more than 2,000 different benzodiazepines have
200 ng/mL Cutoff
been produced, only about 15 are currently FDA-cleared in
the United States. They are usually classified by how long their
Semi-Quantitative or Qualitative Testing
effects last.
Ultra-short acting – midazolam (Versed®), triazolam (Halcion®)
Accurate and Reliable
Short-acting – alprazolam (Xanax®), lorazepam (Ativan®)
Long-acting – chlordiazepoxide (Librium®), diazepam (Valium®)
Liquid, Ready to Use
Common Brand Names: Xanax®, Valium®, Ativan®, Serax®
Abuse Potential: Benzodiazepines have a high potential
for abuse, especially when used with other depressants.
This abuse is partially related to the toxic effects that they
produce and also to their widespread availability. They
can be chronically abused or, as seen more commonly in
hospital emergency departments, intentionally or accidentally
taken in overdose. Death and serious illness rarely result from
benzodiazepine abuse alone; however, they are frequently
taken with either alcohol or other medications.
Versed® and Librium® are registered trademarks of Roche Laboratories, Halcion® and Xanax® are registered trademarks of Pharmacia & Upjohn, Ativan® is a registered trademark of American Home
Products Corp., Valium® is a registered trademark of Roche Products, Serax® is a registered trademark of Wyeth Laboratories.
Tel 909.482.0840 | Toll Free 888.664.8378 | Fax 909.482.0850
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ISO 13485:2003
CERTIFIED
Benzodiazepines
(HEIA™)
Assay Specifications
Cross-Reactivity
Methodology: Homogeneous Enzyme Immunoassay
Cutoff: 200 ng/mL Calibrator Range: 0-1000 ng/mL
Analyte
Analyte Conc.
(ng/mL)
Oxazepam
α-hydroxyalprazolam
Alprazolam
7-Aminoclonazepam
7-Aminoflunitrazepam
7-Aminonitrazepam
Bromazepam
Chlordiazepoxide
Clorazepate
Clobazam
Clonazepam
Demoxepam
Desalkylflurazepam
Diazepam
Estazolam
Flunitrazepam
Flurazepam
Lorazepam
Lorazepam Glucuronide
Lormetazepam
Medazepam
Midazolam
Nitrazepam
Norchlordiazepoxide
Nordiazepam
Oxazepam Glucuronide
Prazepam
Temazepam
Temazepam Glucuronide
Triazolam
Nominal Distribution %
Overlap: Benzodiazepines (200 ng/mL Cutoff)
Rate (ΔmA/min)
Recovered Concentration (ng/mL)
Analytical Recovery: Benzodiazepines
Benzodiazepines
Equiv. (ng/mL)
200
110
100
90,000
4,500
30,000
650
2,000
225
550
200
6,000
85
125
275
150
165
100
375
60
550
50
750
2,000
180
1,500
125
125
700
70
Cross-Reactivity
(%)
200
188
193
213
212
191
198
185
184
212
192
187
194
191
212
208
199
205
213
205
200
203
187
195
217
194
187
197
195
210
100.00
170.90
193.00
0.20
4.70
0.60
30.50
9.30
81.80
38.50
96.00
3.10
228.20
152.80
77.10
138.70
120.60
205.00
56.80
341.70
36.40
406.00
24.90
9.80
120.56
12.90
149.60
157.60
27.90
300.00
No interference was observed by the addition of concentrations up to 100,000 ng/mL of the following potentially interfering
substances. Acetaminophen, Acetylsalicylic Acid, Benzoylecgonine, Caffeine, Diphenhydramine and Methadone were added up to
500,000 ng/mL.
Spiked Concentration Benzodiazepines (ng/mL)
LC-MS/MS Confirmation
(200 ng/mL)
HEIA
(200 ng/mL)
Positive
Negative
Positive
25
0
Negative
8
47
ORDER - Benzodiazepines (HEIA)
4-bromo-2,5-dimethoxyphenethylamine, 6-Acetylcodeine, 6-Acetylmorphine, Acetaminophen, Acetylsalicyclic Acid, Amitriptyline,
Amobarbital, S-(+)-Amphetamine, Benzoylecgonine, Benzylpiperazine, Buprenorphine, Bupropion, Butabarbital, Caffeine,
Carbamazepine, Chlorpromazine, cis-Tramadol, Clomipramine, Cannabidiol, Cannabinol, Carisoprodol, Cocaine, Codeine, Cotinine,
Cyclobenzaprine, Delta-9-THC, Desipramine, N-desmethyltapentadol, Dextromethorphan, Dihydrocodeine, Diphenhydramine,
Doxepin, Ecgonine, Ecgonine methyl ester, EDDP, 1R,2S(-)-Ephedrine, 1S,2R(+)-Ephedrine, Ethyl β-D-glucuronide, Ethylmorphine,
Fenfluramine, Fentanyl, Fluoxetine, Heroin, Hexobarbital, Hydrocodone, Hydromorphone, 11-hydroxy-delta-9-THC, Ibuprofen,
Imipramine, Ketamine, Lamotrigine, Levorphanol Tartrate, Lidocaine, LSD, Maprotiline, (+)-MDA, MDEA, MDMA, Meperidine,
Meprobamate, Methadone, S(+)-Methamphetamine, Methaqualone, Methylphenidate, Morphine, Morphine-3-glucuronide,
Morphine-6-glucuronide, Nalorphine, Naloxone, Naltrexone, Norbuprenorphine, Norcodeine, Normorphine, Norpropoxyphene,
Norpseudoephedrine, Nortriptyline, Oxycodone, Oxymorphone, PCP, Pentazocine, Pentobarbital, Phentermine, Phenobarbital,
Phenylephedrine, Phenylpropanolamine, Phenytoin, PMA, Proproxyphene, Propranolol, Protriptyline, R,R(-)-Pseudoephedrine,
S,S(+)-Pseudoephedrine, Ranitidine, Ritalinic Acid, Salicylic Acid, Secobarbial, Sertraline, Sufentanil Citrate, 11-nor-9-carboxy THC,
Theophylline, Thioridazine, Trifluoromethylphenyl piperazine, Trimipramine, Trazodone, Venlafaxine, Zolpidem Tartrate
Semi-Quantitative Precision (200 ng/mL)
Interday Precision (N = 80)
Concentration (ng/mL)
Mean Concentration (ng/mL)
CV%
50
47.0
12.4
100
99.7
6.2
150 Control LOW
145.2
4.6
Catalog # Description
200
202.6
3.5
314UR-0025 / -0100 / -0500
25 mL / 100 mL / 500 mL Kit
250 Control HIGH
268.1
3.6
300
308.6
2.7
350
347.5
2.7
400
398.9
2.5
For calibrator and control set information, please refer to the
product insert.
For Forensic Use Only
Information on Qualitative Testing is provided in the product insert.
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