WHITE PAPER: EPOC Validation of the LifeQ solution for estimating EPOC April 2015 WHITE PAPER: EPOC Introduction Excess post-exercise oxygen consumption (EPOC) is the additional oxygen consumption required by the body after activity to return the body to its resting state and is measured in ml oxygen consumed per kg of body weight. The excess oxygen consumed during this recovery period is used in several processes aimed at returning the body to resting state homeostasis. For example, physiological variables such as hemoglobin re-oxygenation, phosphagen store replenishment, lactate removal, restoration of muscle glycogen stores, hormone balancing, and cellular repair are all EPOC dependent. EPOC therefore serves to repay the oxygen debt incurred during exercise. assessing and modulating an individual’s training routine as well as the response to varying exercise regimes. Furthermore, variations from normative EPOC values may indicate illness or overtraining and therefore the negative physiological impact of training under these conditions may be prevented. Traditionally, EPOC is measured by analyzing respiratory gas exchange in a laboratory setup. To do this, the area between resting VO2 and the VO2 recovery period is quantified (Fig. 1A). This measurement is used at the “gold standard” benchmark for this white paper for the comparison of estimated values. EPOC is useful in assessing the following, for all types of exercise: • • • • • The body’s rest requirements. Recovery status from previous workout sessions. The physical effect of an entire training routine. Exercise and recovery energy expenditure. Balancing the level of effort to increase fitness without overtaxing the system. Given the above-mentioned applications, EPOC can be used as a tool to assess the achievement of workout goals. For example, an individual performing the same type of exercise at the same intensity for the same amount of time each day should observe a decrease in their EPOC, as well as a faster recovery time, as their cardiorespiratory fitness increases. Additionally, because individuals respond differently to exercise, EPOC is useful for Figure 1: Measured EPOC (area under VO2 recovery period) in ml/kg from a typical increasing intensity exercise period and subsequent rest period. The red dot depicts peak EPOC values. A disadvantage of this is that EPOC can only be assessed upon completing the exercise routine and therefore does not allow for continuous monitoring of the training effect. For example, in Figure 1A, with the traditional WHITE PAPER: EPOC Methods The LifeQ EPOC solution was evaluated by comparing LifeQ predicted EPOC values (using heart rate measurements) to that of EPOC values, as determined by respiratory gas exchange analysis. For this evaluation, due to experimental constraints, the LifeQ EPOC solution was only evaluated from the point of exhaustion (Fig.1A, point C). The validity of LifeQ’s EPOC solution was evaluated with 24 healthy individuals who each completed a VO2max test. For this test, each participant began walking on a treadmill at a speed of 1km/h, which was incrementally increased by 1km/h each minute until 8km/h after which the speed increased every three minutes, until the participant reached exhaustion. After the test, the participants were asked to sit quietly for a resting period of 25 minutes. In addition, before the VO2max test was performed, resting heart rate and respiratory gases were also measured. The LifeQ EPOC solution has an average expected error of 21.9% and a SD of 14.4%, at the point of exhaustion, when compared with traditional gold standard EPOC measurements (Fig. 2 Top graph). Furthermore, for comparison, EPOC was estimated by a commercially available EPOC estimation application solution that is utilized by the Suunto Movescount application (http://www.movescount.com/apps/app10017 721-EPOC_estimation) and validated against the same gold standard This had an average expected error of 31.2% and a SD of 23.4% (Fig. 2 Bottom graph). 250 EPOC as predicted by the LifeQ solu8on (ml/kg). As an alternative to the traditional method of determining EPOC, the LifeQ solution is able to predict EPOC from heart rate derived information as the LifeQ solution can estimate VO2max values from heart rate measurements with 93.3% accuracy (LifeQ White Paper, Validation of the LifeQ solution for measuring VO2max, February 2015). Using heart rate information the LifeQ solution is able to provide EPOC predictions for points A, B, and C without disrupting the exercise routine and thus the EPOC estimation can be monitored in real time (Fig. 1B). Key findings r2 = 0.5678 200 150 100 50 0 0 250 EPOC a per Movescount solu8on (ml/kg). method of measuring EPOC it would not be possible to measure EPOC at points A and B without disrupting the entire exercise routine. Therefore it is only possible to determine EPOC at point C. EPOC as measured by respiratory gas 50 exchange 100 (ml/kg). 150 200 250 r2 = 0.4252 200 150 100 50 0 0 EPOC as measured by respiratory gas 50 exchange 100 (ml/kg). 150 200 250 Figure 2: Correlation between EPOC values as determined by the LifeQ (Top) and Movescount (Bottom) EPOC solutions and by means of respiratory gas exchange. WHITE PAPER: EPOC At this point we cannot yet test the error levels continuously as there is no laboratory test available and as such the the accuracy of the LifeQ solution is assumed for EPOC estimations at any point during the exercise routine to be similar to the exhaustion point measure validating here. In addition, the LifeQ EPOC solution also allows for the monitoring of EPOC during exercise recovery, (Fig. 3) allowing an individual to ensure full recovery before their next exercise routine. Figure 3A illustrates the decline in EPOC after maximum exhaustion as determined by respiratory gas exchange and figure 3B shows the corresponding EPOC decline as estimated by the LifeQ EPOC solution. Figure 3: Comparison of the decline in EPOC following the point of maximum exhaustion (red dot) as determined by (A) respiratory gas exchange and (B) the LIfeQ EPOC solution. In conclusion, the LifeQ EPOC solution provides the only viable solution for quantifying an individual’s continuous EPOC in real time.
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