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Course Overview
Biology of Mammalian Cells and
Tissues - Histology
VIBS 443/ VIBS 602
Larry Johnson
Cells
Tissues
Organs/systems
Objectives
To make a transition from
molecular events to organ
systems.
To have classical histology
merged with modern cell biology.
To learn technical skills - new types of vision - using
the light microscope and electron microscope.
Objectives
To learn the relationship
between cell structure and cell
function, and how organ
architecture facilitates its function.
To learn normal histology as a prerequisite for
understanding pathology.
To learn how to communicate and have fun with
histology.
Materials
Laboratory notebook file,
study guides, and items to identify
Course schedule
Computers to view histology images, electron
micrographs, and their care
PowerPoint slides to introduce each laboratory,
conference, and lecture
Labeled electron micrographs
Atlas images
diFiore’s Atlas of Histology w/ Functional Correlations 12th edition
Introductory to labs YouTube
VIBS Histology
Introductory to labs PPTs
Introductory to labs PPTs
VIBS Histology
Introductory to labs PPTs
VIBS Histology
To view the Imagescope files on
students computers in computer labs
1. Login as a vet or grad student or if undergrad, use the
ID of “cvmlab” with the password of “AStud3nt”.
2. Wait for the icons on the lower right corner of the task
bar to appear (usually around 5 icons).
3. Click on Start, VIBS folder, Imagescope
4. Once the program loads, Click on File, Open Image
5. Change to the X:\classes drive
6. Click on "Shortcut to
LJohnsonHistologySlides”
7. Choose the file you want to view.
Hence, you will login, load program,
load files, and select file.
Materials
Lecture outlines, laboratory exercises, word slides,
and study guides for each lecture. [Note that some
of these materials are modified from copyrighted
textbooks (see last page for original sources) and
should not be copied for non-personal use.]
Evaluations - must be returned to receive final exam.
Materials
Materials
Textbooks
• Junqueira’s Basic histology Text & Atlas by
Anthony L. Mescher 13e (ISBN 978-0071440912)
• Earlier versions will likely work too as the textbook
materials will be used as a supplement source of
information.
Organization
Cell Biology - Tissues - Organs - Organ Systems
Lectures: (26) Begin usually at 10:20 A.M. are
supplemented by five clinical correlations to relate
lectures material to pathological conditions. The
objective of lectures is to learn about structure function relationships and fundamental concepts.
Laboratories: To learn cell and tissue appearance
and function. Subject matter of laboratories usually
will be that material covered in lecture of the
previous class period.
Conferences: (10) To develop conceptual thinking for
problem solving.
Organization
Preparative homework allows the student to
become familiar with the subject of the lecture
prior to the lecture period.
Preparative homework (25 sets total) is due prior
to the lecture on the day that the subject matter
is covered in the lecture.
Organization
The homework will consist of a list of 3 to 5
structures or structural features unique or
specifically characteristic of cells, tissues,
organs, or organ systems covered in the lecture
that day, and one sentence for each describing
how these structures or structural features
contribute to the unique function of that given
cell, tissue, organ, or organ system.
Possibly, a randomly-selected subset of
homework will be graded; however, all
homework submitted on time will be recorded.
Parts of course will be FLIPPED
for more class time discussions.
You will watch short
videos in preparation
for class.
• Lab introductions – to set up the
observation of images as directed within
the lab manual
• Introductions to conferences
• Lab introductions – to set up the
observation of images as directed within
the lab manual
• Introductions to conferences
FLIP = FLoating
Instrument
Platform
Exams:
Quiz to test all materials covered since last
quiz or didactic test
Practical exams to test concepts and
identifications
Essay exams on conference material
Didactic exams to test everything
Writing Assignments
Term Paper Objectives
• To advance understanding of
conference topics as they relate to
real-world situations
• To improve communication skills of
students in medical fields
• To improve awareness of issues
related to common diseases
Writing Assignments
Term Paper Prompt
Choose one of the discussion conference topics about which
to compose your essay. Assume this will be published in a
scientific journal and will be read by your colleagues. Use
outside sources, which should be cited correctly at the end
of the paper, and describe the implications of your topic to
the medical field.
The final product should be 2000+ words (not to exceed 3,500
words), typed and double spaced, in Times New Roman,
with 1” margins. Be sure to include the title of the
discussion conference in the paper and a list of your
sources at the end. If you use a direct quotation, you must
correctly cite it in the paper.
Writing Assignments
Submittals
The following will be due throughout the semester.
Feedback will be given at each step, with a final grade
given for the final draft.
Outline
Description
First Draft
Second Draft
Final Draft
Grading of Term Paper
Submitting of each paper on time
10 points
First and Second Draft:
40 points
Final Draft:
100 points
Total points of course:
150 points
H. Grading
1. Point distribution
Point Values
a.
Preparative homework
100
b.
Quiz (6) every other week
200
(50 points each, drop 2)
c.
Term Paper
150
d.
Mid-term
i.
Practical portion
100
ii.
Conference essay portion 25
iii.
Didactic portion
150
e.
Final
i.
Practical portion
100
ii.
Conference essay portion 25
iii.
Didactic portion
150
Total
1000
2. A=900+; B=800-899; C=700-799; D=600-699; F=599-0
Organization
Student participation in class and student
enhancement of the objectives of the course are
encouraged and may pay off in borderline cases.
Scholastic dishonesty including plagiarism as defined
at the TAMU Web Site (http://studentrules.tamu.edu/rules20.htm) will not be allowed.
Grades may be curved at the end of the semester.
Getting Started
in the
Laboratory
Light microscope parts
Kohler illumination
page 28
field diaphragm
Kohler illumination
1. Focus on the tissue specimen using
the course/fine focus knobs.
2. Close the field diaphragm.
3. Focus the condenser (e.g. , image of the closed field
diaphragm) on the specimen using the condenser height
adjustment knob.
4. Center the condenser (e.g., image of the field diaphragm)
with the two condenser centering knobs so that the light
passing through the opening of the field diaphragm is
concentric with the peripheral edges of view.
5. Open the field diaphragm so that field diaphragm is just
outside of view.
Kohler illumination
1 Focus on
the tissue
4 Center the
condenser
1. Focus on the
tissue.
2. Close the field
diaphragm.
3. Focus the
condenser.
3 Focus the condenser
2 Close the
field diaphragm
Open the
5
field diaphragm
4. Center the
condenser
5.
Open the field
diaphragm.
Cartilage growth in endochondral bone
220 formation of the Fetal finger
105
Fingertip, monkey - sweat glands
and ducts among Pacinian corpuscles
209
Skin, scalp
136
Skeletal muscle of Tongue,
monkey
107
Skin, melanocytes, guinea pig (DOPA reaction)
Birds on the Move
Backgrounds
Rockport
Birds on
the Wing