Document

CPA300™ fully automated puncher
Get quality results with excellent throughput
and minimal manual intervention
The Card Processing Automation 300 (CPA300™) Instrument is
a fully automated system that integrates sample card punching,
liquid handling, and 96-well plate sealing for forensic, paternity,
and research applications.
The instrument offers:
• Automatic detection of deposited samples (saliva/blood) using a high-resolution
camera and integrated software analysis
• Camera control performed on each well before and after punching to help reduce
the risk of errors
• Traceability of samples through barcode reading
• User-friendly software interface
• Liquid handling module for automatic dispensing of PCR reagents
• Reprocessing of already punched positions
• Integrated sealing unit
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Card Processing Automation (CPA300™) Instrument
Punching
accuracy
• Ionizer to remove static
• Vacuum system to remove debris
• Cleaning punch functionality
• Camera recognition of blood and
buccal samples
System
flexibility
• Works with standard and custom
framed cards
• Protocol optimized for NUCLEICCARD™ sample collection devices
with dedicated cleaning area to help
improve efficiency and help reduce
time to results
• Liquid dispensing unit to dispense
PCR reagents for STR amplification
• Plate sealing capabilities to help
improve efficiency
Full
traceability
• Plate and card barcode reading
• Camera control of each well before
and after punching
• Real-time updates on plate status
• Customizable reports
Card Processing Automation (CPA300™) Instrument
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Specifications and features
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Supported card formats
The CPA300™ Instrument works with framed NUCLEIC-CARD™ sample collection devices (Copan) and FTA® cards (Whatman)
sample collection systems routinely used in forensic laboratories, and both indicating cards (saliva samples) and nonindicating
cards (blood samples).
The CPA300™ Instrument can reprocess already-punched cards.
Using the framed NUCLEIC-CARD™ sample collection devices with cleaning area:
Optimized protocol
1-spot format: 51 mm x 51 mm
2-spot format: 85 mm x 53 mm
The CPA300™ Instrument has the following standard punch configurations:
1.2 mm**
2 mm
3 mm
4 mm
Available punch sizes
Sample detection
Automatic sample detection (saliva or blood)
Cleaning procedure
Optimized protocol using the NUCLEIC-CARD™ sample collection devices with integrated cleaning area.
Multiple cleaning strikes in between punches.
Capacity
Fully automated: 3 plates (288 cards)
Camera features and imaging
Model: Allied Vision Technologies Manta
Resolution: 2,452 x 2,056
Sample presence control: Automatic comparison between the well picture before punching (well normally empty) and after
punching (well normally full). Verification of the empty/full well before proceeding with the next punch.
Image storage: All acquired images are saved to a specific directory on the PC desktop.
Physical dimensions
Including PC: 1,700 mm (L) x 760 mm (W) x 1,005 mm (H)
Operational box: 1,160 mm (L) x 760 mm (W) x 1,005 mm (H)
Weight: 270 kg
PC hardware and software
The computer is an industrial PC embedded inside the instrument: only the 19” touch screen, mouse, and keyboard are visible to
the user. The computer has a dual-core 2.2 GHz CPU, 4 GB RAM, and a 32 GB solid-state drive. It runs on the Linux® Ubuntu®
operating system.
Peripherals: 19’’ touch screen/keyboard/mouse/USB (2)
Communication: USB/data transfer
Network: Ethernet (1 Gb/s)
LIMS integration
The instrument can be connected to LIMS in order to import and export files.
Exportable files
The instrument can export files in formats suitable for the 3130 and 3500 Genetic Analyzer sequencers, and can export reports
as PDF files.
Main power requirements
1,200 W (peak) electrical power; 110-240 VAC main supply voltage; 50/60 Hz frequency
Pollution degree
2
Overvoltage category
2
Pipetting unit
1 pipetting channel
Tip sizes: 50 µL, 1,000 µL
Tip station: dedicated area for up to three 96-tip racks
Reagent station: 4 slots with optimized temperature settings
Refer to CPA300™ Instrument technical documents for additional information on specifications and validated protocols.
A version of the CPA300™ Instrument without the sealing unit is available.
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The CPA300™ Instrument comes with 1.2 mm punch sizes by default.
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Ordering information
Product
Cat. No.
CPA300 Instrument
A26355
CPA300™ Instrument (without sealing unit)
A26041
™
Find out more at lifetechnologies.com/samplepuncher
For Research, Forensic, or Paternity Use Only. Not for use in diagnostic procedures.
© 2015 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property
of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. FTA is a
trademark of Whatman, Ltd. Copan, CPA300 and NUCLIEC-CARD are trademarks of
Copan Italia S.P.A. Ubuntu is a registered trademark owned by Canonical Ltd. Linux
is a registered trademark owned by Linus Torvald. CO013340 0115