Ready to break free from your multiple cloning site?
Ready to break free from your multiple cloning site? In‑Fusion® Cloning lets you choose any locus on any vector. Why waste time trying to design around available restriction sites? Put away the restriction enzymes and ligase, and take full control of your experiment. Clone your insert exactly where you want, the first time, and move forward with your research. Cloning free of restrictions: • • • • Any insert, any vector, any locus Directional, ligase-free reactions No subcloning required High accuracy and low background See how those in the field have used In-Fusion Cloning to overcome restriction site availability. 20% off list price Item Item description Package size List price 638916 In-Fusion® HD Cloning Plus CE 10 rxns $476 +$47.6 GST 638917 In-Fusion® HD Cloning Plus CE 50 rxns $1,901 +$190.1 GST $1,520.0 +$152.00 GST 638919 In-Fusion HD Cloning Plus CE 96 rxns $3,063 +$306.3 GST $2,450.4 +$245.04 GST 638918 In-Fusion HD Cloning Plus CE 100 rxns $3,208 +$320.8 GST $2,566.4 +$256.64 GST 638912 In-Fusion® HD EcoDryTM Cloning Plus 8 rxns $419 +$41.9 GST $355.2 +$35.52 GST 638913 In-Fusion® HD EcoDryTM Cloning Plus 24 rxns $1,036 +$103.6 GST $828.8 +$82.88 GST 638914 In-Fusion® HD EcoDryTM Cloning Plus 48 rxns $2,046 +$204.6 GST $1,636.8 +$163.68 GST 638915 In-Fusion® HD EcoDryTM Cloning Plus 96 rxns $2,965 +$296.5 GST $2,372.0 +$237.2 GST 638909 In-Fusion® HD Cloning Plus 10 rxns $461 +$46.1 GST $368.8 +$36.88 GST 638910 In-Fusion HD Cloning Plus 50 rxns $1,790 +$179.0 GST $1,432 +$143.2 GST 638920 In-Fusion HD Cloning Plus 96 rxns $2,982 +$298.2 GST $2,385.6 +$238.56 GST 638911 In-Fusion® HD Cloning Plus 100 rxns $3,098 +$309.8 GST $2,478.4 +$247.84 GST ® ® ® ® Discount price Freight charges apply. Please contact Scientifix for details. Prices subject to change without notice. Valid till end of May 2015 Valid for initial purchase only. InFusion20OFF Click to view the data 1800 007 900 [email protected] Click to contact Scientifix scientifix.com.au $380.8 +$38.08 GST In-Fusion Cloning: ® The right clone every time. Good science relies on a solid foundation of inspiration matched with technical expertise. Every decision has an impact, starting with experimental design. This is why we are dedicated to delivering best-in-class products backed by expert scientific support. We understand that streamlined methods and high-performance reagents enable you to push your research to its fullest potential. Ideal for... • Single-insert cloning • Multiple-insert cloning • Large-insert cloning • Adding small tags • Direct cloning into large vectors (up to 46-kb cosmids) • Site-directed mutagenesis (single or multiple deletions, insertions, or substitutions) In-Fusion Cloning kits are unique in their ability to allow easy, accurate, seamless cloning without the use of ligase. From the simple to the complex, you can recover your final construct on the second day! In just one quick reaction, you can clone any insert into any vector at any locus, without the hassle of subcloning, unwanted extra bases, or inconvenient restriction sites. The reaction is specific and directional, ensuring an exceptionally high rate of cloning accuracy in all applications. Our standards for accuracy involve more than simple transformation efficiency. Why waste time screening a large number of colonies? With background levels approaching zero, you can pick just a few colonies and be confident that you have the right clone. As the first step in so many experiments, successful cloning is key to recovering meaningful data. The cloning tools you use can be the difference between wasted time and fast, consistent results. Amplify your gene of interest PCR product with 15 bp extensions complementary to the vector ends In-Fusion enzyme, vector, and PCR product Any vector Desired construct 15 min (liquid) or 30 min (EcoDry) single-tube reaction Figure 1. In-Fusion Cloning protocol. The key to In-Fusion Cloning is 15 bp of homology between your insert(s) and linearized vector backbone. Add these 15 bp to the 5’ end of your forward and reverse primers, and PCR amplify your desired insert(s). Linearize your vector, by restriction digest or inverse PCR. Combine in a single tube containing the In-Fusion enzyme mix, perform the In-Fusion cloning reaction, and transform into competent cells.
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