TARGETED RESEQUENCING
20-25%
UPSIZE or
20% OFF
TaKaRa PrimeSTAR GXL DNA Polymerase
& LA Taq HS DNA Polymerase
Behind every good targeted resequencing experiment, there’s a great polymerase
Because targeted resequencing using Next Generation Sequencing (NGS) platforms allows detailed investigation
of specific genomic regions, this technique is frequently used to assess somatic or germline mutations. Targeted
resequencing also aids discovery of novel causative mutations for genetic disorders. DNA samples are amplified by an
initial round of PCR, then pooled for library preparation and NGS. This first amplification step is critical to success. For
targeted resequencing workflows with a variety of NGS platforms we offer PrimeSTAR GXL DNA Polymerase and TaKaRa
LA Taq HS DNA Polymerase.
Applications
Product Length
Fidelity
High % GC template
PrimeSTAR GXL
•
•
•
•
Point mutations
GC-rich, AT-rich, or repetitive
sequences
Rare alleles
Limiting sample amounts
•
•
≤ 30kb (genomic) High
≤13.5 kb (cDNA) (1 error/16,230 bp tested on GC-richThermus thermophilus
HB8 genomic DNA)
•
≤ 30kb (genomic) Moderate
(approx.6.5X higher fidelity than
Taq)
Recommended
TaKaRa LA Taq HS
•
•
•
•
Breakpoint regions
T cell receptor loci rearrangements
mtDNA mutations
Genome variation
Item
Description
Size
Price
Free upsize
R050A
PrimeSTAR GXL DNA
Polymerase
250 units
$376 + $37.6 GST
50 units (R050Q)
1000 units
$1,177 + $117.7 GST
250 units (R050A)
TaKaRa LA Taq HS
125 units
$338 + $33.8 GST
20% Off
500 units
$1,245 + $124.5 GST
125 units (RR042A)
R050B
RR042A
RR042B
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information
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contact Scientifix
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Quote: 20offReSeq. Offer valid till the end of May, 2015
Freight charges apply. Please contact Scientifix for details. Prices subject to change without notice.
1800 007 900
[email protected]
www.scientifix.com.au
T R A N S L AT I O N A L
TGS Seal Accent
R E S E A R C H
CMYK & Lab numbers
CITATION LISTS:
Precison-Tuned PCR for
Translational Research
Process Cyan
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In translational research, every decision has an impact—including the decision
about which PCR polymerase to use for your assays. Learn about peer-reviewed
studies in which scientists selected Takara® and Clontech® polymerases for
biomedical and preclinical research.
Targeted resequencing interrogates a specific genomic region
of interest by NGS, allowing rapid investigation. The choice of2617
8/100/80/3
polymerase used for amplification is critical.
45 68 40
www.clontech.com/targeted-resequencing
Single copy PCR is often used for routine screening for a target that is
present at low copy number—even as low as a single copy per sample.
www.clontech.com/single-copy-pcr
369
Multiplex PCR assays are designed to detect a suite of targets
at once,
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increasing efficiency by generating more data per reaction.
www.clontech.com/multiplex-pcr
Fast PCR enables screening in a fraction of the time by using
high-speed enzymes that allow swift, accurate sample analysis.
www.clontech.com/fast-pcr
Direct PCR allows amplification from blood, cells, or tissue without
DNA extraction, decreasing labor and expediting conclusions.
www.clontech.com/direct-pcr
Methylation analysis uses techniques such as methylation-specific
PCR, bisulfite sequencing, or COBRA to analyze DNA methylation
patterns affecting aging, cancer, or other diseases.
www.clontech.com/methylation-pcr
Best-in class PCR polymerases for success
in technically demanding applications.
Learn more today
Clontech Laboratories, Inc. • A Takara Bio Company
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.543.7247
For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resale. Takara and the Takara logo are trademarks of TAKARA
HOLDINGS INC., Kyoto, Japan. Clontech, the Clontech logo, and that’s GOOD science! are trademarks of Clontech Laboratories, Inc. All other marks
are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions. ©2014 Clontech Laboratories, Inc.
www.clontech.com
9.14 IN (633718)