TARGETED RESEQUENCING 20-25% UPSIZE or 20% OFF TaKaRa PrimeSTAR GXL DNA Polymerase & LA Taq HS DNA Polymerase Behind every good targeted resequencing experiment, there’s a great polymerase Because targeted resequencing using Next Generation Sequencing (NGS) platforms allows detailed investigation of specific genomic regions, this technique is frequently used to assess somatic or germline mutations. Targeted resequencing also aids discovery of novel causative mutations for genetic disorders. DNA samples are amplified by an initial round of PCR, then pooled for library preparation and NGS. This first amplification step is critical to success. For targeted resequencing workflows with a variety of NGS platforms we offer PrimeSTAR GXL DNA Polymerase and TaKaRa LA Taq HS DNA Polymerase. Applications Product Length Fidelity High % GC template PrimeSTAR GXL • • • • Point mutations GC-rich, AT-rich, or repetitive sequences Rare alleles Limiting sample amounts • • ≤ 30kb (genomic) High ≤13.5 kb (cDNA) (1 error/16,230 bp tested on GC-richThermus thermophilus HB8 genomic DNA) • ≤ 30kb (genomic) Moderate (approx.6.5X higher fidelity than Taq) Recommended TaKaRa LA Taq HS • • • • Breakpoint regions T cell receptor loci rearrangements mtDNA mutations Genome variation Item Description Size Price Free upsize R050A PrimeSTAR GXL DNA Polymerase 250 units $376 + $37.6 GST 50 units (R050Q) 1000 units $1,177 + $117.7 GST 250 units (R050A) TaKaRa LA Taq HS 125 units $338 + $33.8 GST 20% Off 500 units $1,245 + $124.5 GST 125 units (RR042A) R050B RR042A RR042B Click for more information Click here to contact Scientifix Click to view the case study Quote: 20offReSeq. Offer valid till the end of May, 2015 Freight charges apply. Please contact Scientifix for details. Prices subject to change without notice. 1800 007 900 [email protected] www.scientifix.com.au T R A N S L AT I O N A L TGS Seal Accent R E S E A R C H CMYK & Lab numbers CITATION LISTS: Precison-Tuned PCR for Translational Research Process Cyan 78/0/0/0 67 -36 -44 In translational research, every decision has an impact—including the decision about which PCR polymerase to use for your assays. Learn about peer-reviewed studies in which scientists selected Takara® and Clontech® polymerases for biomedical and preclinical research. Targeted resequencing interrogates a specific genomic region of interest by NGS, allowing rapid investigation. The choice of2617 8/100/80/3 polymerase used for amplification is critical. 45 68 40 www.clontech.com/targeted-resequencing Single copy PCR is often used for routine screening for a target that is present at low copy number—even as low as a single copy per sample. www.clontech.com/single-copy-pcr 369 Multiplex PCR assays are designed to detect a suite of targets at once, 68/20/100/8 49 -29 40 increasing efficiency by generating more data per reaction. www.clontech.com/multiplex-pcr Fast PCR enables screening in a fraction of the time by using high-speed enzymes that allow swift, accurate sample analysis. www.clontech.com/fast-pcr Direct PCR allows amplification from blood, cells, or tissue without DNA extraction, decreasing labor and expediting conclusions. www.clontech.com/direct-pcr Methylation analysis uses techniques such as methylation-specific PCR, bisulfite sequencing, or COBRA to analyze DNA methylation patterns affecting aging, cancer, or other diseases. www.clontech.com/methylation-pcr Best-in class PCR polymerases for success in technically demanding applications. Learn more today Clontech Laboratories, Inc. • A Takara Bio Company United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.543.7247 For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resale. Takara and the Takara logo are trademarks of TAKARA HOLDINGS INC., Kyoto, Japan. Clontech, the Clontech logo, and that’s GOOD science! are trademarks of Clontech Laboratories, Inc. All other marks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions. ©2014 Clontech Laboratories, Inc. www.clontech.com 9.14 IN (633718)
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