EKSIGENT MICROLC 200 PLUS SYSTEM Boost your LC/MS Throughput and Sensitivity EKSIGENT MICROLC 200 PLUS Meet the Industry Pioneer High-Throughput Microflow UHPLC. For Research Use Only. Not for use in diagnostic procedures. As an industry pioneer in microflow UHPLC, SCIEX understands that sensitivity, throughput, and cost of ownership are of the utmost importance for highthroughput LC/MS/MS laboratories. The Eksigent MicroLC 200 Plus System is engineered for high-throughput LC/MS/MS and delivers: • Up to 10x better LOQ and 4x throughput compared to 2.1 mm workflow • Up to 90% solvent savings • Stackable design that saves space • Software designed to support 21 CFR Part 11 compliance • Trusted IQ/OQ/PQ service available High Throughput Microflow UHPLC The Eksigent MicroLC 200 Plus System robustly increases the throughput and sensitivity of mass spectrometry platforms. In addition to increasing throughput, the microflow The Eksigent MicroLC 200 Plus System is fully The system has a minimum dead volume flow path rate regime of the Eksigent MicroLC 200 Plus System optimized for integration with the SCIEX mass and low dispersion hybrid electrodes designed for can also improve the sensitivity performance of mass spectrometry portfolio with operational control the Turbo V™ and IonDrive™ Turbo V sources. These spectrometers. This makes the Eksigent MicroLC 200 through Analyst Software. The software is are incorporated in a small functionally stackable Plus System a sound strategy when added LC/MS/MS designed to support 21 CFR Part 11 compliance. footprint for integration with the SCIEX 4500, 5500 sensitivity is needed. SCIEX also offers a full IQ/OQ/PQ service for the and 6500 series mass spectrometers. ® MicroLC 200 Plus. For Research Use Only. Not for use in diagnostic procedures. Achieve the Throughput of Multiplexed LC/MS Systems Modern hardware, powerful software, and robust engineering increase your productivity to routinely analyze hundreds of compounds in hundreds of samples – day after day, year after year. Increase sensitivity and throughput – reduce sample Accelerated throughput volume and solvent consumption Scientists who need to increase throughput while For example, peptide quantitation applications Sensitivity is always important. In addition in simultaneously improving the robustness of their migrating to the MicroLC 200 Plus from nano many applications, sample volume is limited or assays will appreciate the MicroLC 200 Plus binary flow based methods stand to gain up to 10X incurred sample re-analysis (ISR) strategies have gradient pumping system. The system is designed to higher throughput and significantly improved to be considered. support columns of 0.3 mm – 1 mm ID. The system robustness compared to traditional nano flow significantly reduces the required mixing volumes approaches, while simultaneously improving and produces impressive gradient delays of 1-3 μL – gradient speed and precision. In these situations, the all new Eksigent MicroLC 200 Plus System excels – with up to 10X more sensitivity, up to 4X more throughput, and up to 10X less sample volume consumption. Unique microflow with accelerated separations and faster cycle times for complex samples. pumping capabilities make this possible, along with the advanced dynamic load-and-wash of the HTC-xt autosampler and near-zero sample loss injection technology. Increase sensitivity while using less sample Accelerate your throughput while increasing robustness 10 µl injection 100 ~60 to 800 pg on column Less sample, more sensitivity: Separation of 23 4.0e5 3.0e5 pharmaceutical and personal care product compounds in 6.61 2.0e5 water on the Eksigent MicroLC 200 Plus System. With 4X less 1.0e5 sample injected, peak height, peak area and signal to noise 0.00 Nanoflow based method Intensity, cps 6.0e5 5.0e5 20.39 increased by 4X. 2 4 6 8 101214 102030 Time, min 1.00e6 2.36 2.5 µl injection ~15 to 200 pg on column 8.00e5 6.00e5 4.00e5 quantification: Select peptides from a multiplexed assay of P450 peptides in digested liver microsomes were compared between nanoflow and a higher throughput microflow 4X less sample 4X greater sensitivity 2.00e5 0.00 Time, min 2 46810 1214 Time, min For Research Use Only. Not for use in diagnostic procedures. approach on the Eksigent MicroLC 200 Plus. Throughput has been increased by 8X versus the nanoflow experiment for the same sample. 100 Intensity, cps 1.2e6 Increasing throughput and robustness of peptide Microflow based method 8X increase in throughput 2.940 1.02.03.04.05.0 Time, min Unrivaled Microflow UHPLC Solution Optimized for MS/MS System specifications The Eksigent MicroLC 200 Plus System is a dedicated splitless microflow UHPLC system designed to deliver optimal microflow performance and streamlined integration for your most challenging separations – on any mass spectrometry platform. Feature Eksigent MicroLC 200 Plus Dimensions (W x D x H) 25”x 20”x 28”/ 63.5cm x 50.8cm x 71.1cm Weight 55 lbs / 25 kgs Power 100 – 240 VAC Flow Rate Range 5-200 µL/min Maximum Pressure 10,000 PSI Gradient Delay Vol < 1 - 3 µL Flow Rate Precision <0.5% RSD @ 150 µL/min Streamlined integration with MicroLC column At the heart of precision Eksigent MicroLC Columns are available in a wide Proprietary Eksigent Microfluidic Flow Control Flow Rate Accuracy < 1% range of particle sizes (3 and 5μm), pore sizes technology delivers flow rate precision better than Sample Capacity (100 Å and 300 Å) and surface chemistries including 0.5% RSD, making it ideal for small molecule and 6 trays, standard or deep 96-well 384-well microplates Column Oven +5 - 80˚ C C18, C8, C4, CN, Phenyl, AQ, C18-superficially peptide analysis. Operating in a true microflow Injection Volume Range porous particles and Silane (SIL). regime, the MicroLC 200 Plus System is a UHPLC 15 nL - 10 µL (without changing loop)up to 50 µL with optional sample loops Injection Volume Precision <1% RSD (full loop) <2% RSD (partial loop) Mobile Phase Consumption typically < 10 mL/day Carryover less than 0.003% (30ppm) Cycle Time < 60 seconds ™ system that can accommodate sub 1mm ID columns without any hardware changes – and up to 95% savings in mobile phase. Columns and electrodes optimized for high-throughput microflow LC/MS/MS Eksigent packs and sells a wide range of MicroLC column sizes Small electrodes, big advantage: Reduced dispersion electrodes for and phases with hardware and fittings optimized for the Eksigent the TurboV™ and IonDrive™ TurboV sources improve resolution. • Advanced flow path designed for minimum dead volume • Pumping system engineered for reliability and reduced maintenance costs • Combine with microflow rate optimized columns and electrodes MicroLC 200 Plus System. For Research Use Only. Not for use in diagnostic procedures. Your Success is Our Success We take it personally As a SCIEX customer you have access to a world-class customer support organization. Wherever you are, we’re there with you as a trusted partner to answer questions, provide solutions, and maximize lab productivity. Our customer support organization has access to the latest product updates, software revisions, methods and repair procedures to make sure that you stay on top of your game. When you have questions, we have answers. Learn more at www.absciex.com/customersupport, or locate your local account © 2015 AB Sciex. For Research Use Only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners. RUO-MKT-03-1737 1/2015 representative at www.absciex.com/contactus Headquarters 500 Old Connecticut Path Framingham, MA 01701 USA Phone 508-383-7700 www.absciex.com Answers for Science. Knowledge for Life. ™ International Sales For our office locations please call the division headquarters or refer to our website at www.absciex.com/offices
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