Antibody Research Group 1 Mission Statement ∗ To build a network through which those focused on antibody development, particularly within the academic setting, can network to not only learn from each other but also to work together to find solutions to common challenges. 2 Research Group Committee ∗ ∗ ∗ ∗ ∗ ∗ ∗ ∗ David Blum (chair) Frances Weis-Garcia Dennis A. Bagarozzi, Jr. Kathleen Brundage Rob Carnahan Linda Green Philip Gao Chris Colangelo (EB Liaison) 3 EuroMAbNet ∗ Established contact in 2014 ∗ Focus similar to ARG ∗ Protocols ∗ Validation ∗ Planning meeting in Slovenia this year ∗ May have exchange of member visits 4 Previous Year Activities ∗ Survey ∗ Immunization Strategies for Weakly Immunogenic Targets ∗ Screening Strategies for Monoclonal Antibody Development ∗ Antibody Sequencing and Recombinant Antibody Production ∗ The Perfect Antibody 5 Current Activities ∗ ChIP Study ∗ Validating the validation kit ∗ Goal: Develop screening strategy for validating antibodies for ChIP-Seq ∗ Antibody Discussion Forum & Virtual Lab Meeting ∗ Antibody Developer Network 6 Assay Overview ∗ Chromatin: Embryonic mouse hindlind or Control Chromatin ∗ AcH3 Control Antibody ∗ Target: H3S10ph ∗ “Validated Antibody” – MC463 ∗ Samples (Hiroshi Kimura) ∗ 10H12 ∗ CMA3117G1G7 ∗ Epigentek Pre-SureTM Antibody Validation kit 7 ChIP Grade Intensity (CGI) 8 ChIP data 40.0 36.5 35.0 30.0 CGI 25.0 20.0 15.0 10.0 6.1 5.0 4.5 4.5 10H12 CMA3117G1G7 1.0 0.0 EMH Control MC463 9 Future Directions ∗ Collaboration with NARG or other RG for ChIP-Seq study ∗ Chromatin comparison ∗ New antibodies ∗ Sequence comparison of ranked antibodies ∗ Phage Display Technology Development ∗ Rabbit Monoclonals 10
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