Antibody Research Group
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Mission Statement
∗ To build a network through which those focused on
antibody development, particularly within the
academic setting, can network to not only learn from
each other but also to work together to find solutions
to common challenges.
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Research Group Committee
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David Blum (chair)
Frances Weis-Garcia
Dennis A. Bagarozzi, Jr.
Kathleen Brundage
Rob Carnahan
Linda Green
Philip Gao
Chris Colangelo (EB Liaison)
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EuroMAbNet
∗ Established contact in 2014
∗ Focus similar to ARG
∗ Protocols
∗ Validation
∗ Planning meeting in Slovenia
this year
∗ May have exchange of
member visits
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Previous Year Activities
∗ Survey
∗ Immunization Strategies for Weakly Immunogenic
Targets
∗ Screening Strategies for Monoclonal Antibody
Development
∗ Antibody Sequencing and Recombinant Antibody
Production
∗ The Perfect Antibody
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Current Activities
∗ ChIP Study
∗ Validating the validation kit
∗ Goal: Develop screening strategy for validating
antibodies for ChIP-Seq
∗ Antibody Discussion Forum & Virtual Lab Meeting
∗ Antibody Developer Network
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Assay Overview
∗ Chromatin: Embryonic mouse
hindlind or Control Chromatin
∗ AcH3 Control Antibody
∗ Target: H3S10ph
∗ “Validated Antibody” – MC463
∗ Samples (Hiroshi Kimura)
∗ 10H12
∗ CMA3117G1G7
∗ Epigentek Pre-SureTM Antibody
Validation kit
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ChIP Grade Intensity (CGI)
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ChIP data
40.0
36.5
35.0
30.0
CGI
25.0
20.0
15.0
10.0
6.1
5.0
4.5
4.5
10H12
CMA3117G1G7
1.0
0.0
EMH
Control
MC463
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Future Directions
∗ Collaboration with NARG or other RG for ChIP-Seq
study
∗ Chromatin comparison
∗ New antibodies
∗ Sequence comparison of ranked antibodies
∗ Phage Display Technology Development
∗ Rabbit Monoclonals
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