UHID Research Groups
UHID Research Groups January 2010 2 PREFACE For centuries infectious diseases have threatened mankind, and on many occasions they have changed history profoundly. Today, infectious diseases continue to pose enormous challenges to human well being and medical science worldwide. While antibiotics, novel therapeutics, vaccines, new genetic analyses, and sensitive diagnostic procedures have improved our ability to prevent, treat, and control infectious diseases, their elimination will occur only through extensive, collaborative national and international biomedical and public health research. The University Hospital for Infectious Diseases (UHID) is the leading infectious disease clinical and research facility in Croatia and the region. Its infectious disease focus is unique and provides the ability to manage patients harboring a wide variety of pathogens, conduct research with modern diagnostic and analytical tools, teach undergraduate students, and train postgraduate nursing and clinical specialists. This brochure is intended to briefly inform readers about the members and activities of the research groups at the UHID. The experts at UHID would be honored to discuss potential collaborations on infectious disease challenges with colleagues worldwide. Recently, thanks to the support of the Croatian Ministry of Sciences, Education and Sports, a Central Diagnostic Laboratory and the first Croatian Biosafety Level 3 and Biosafety Level 2+ Laboratory were constructed at the UHID. These laboratories will facilitate the involvement of UHID scientists in collaborative projects. In addition, the Ministry has supported the employment of 14 new PhD students at the Infectious Disease Clinic, guaranteeing future clinical and research expertise. In keeping with the longstanding mission of the UHID, its experts participate in many national and international projects. This brochure describes some of the clinical and basic research activities of UHID scientists in different areas including: Nosocomial Infections, Endocarditis, HIV/AIDS, Hepatitis B,C,E, Papillomaviruses, Sexually Transmitted Infections, Urinary Tract Infections, Respiratory Tract Infections (emphasis on Flu), Antimicrobial Therapy and Resistance, Pediatric Infections / Diseases, Infections in the Immunocompromised, Imunopathogenesis of Infections, Emerging and Re-emerging Infectious Diseases, and Tropical / Parasitic diseases. Modern UHID infectious disease research will build on a tradition of success. For example, some human infectious diseases were discovered by UHID scientists. a. Human infection with Bhanja virus. Vesenjak-Hirjan J, Calisher CH, Beus I, Marton E. First natural clinical human Bhanja virus infection. Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. I. Abt. 1980; (suppl. 9):297-301. (Vesenjak-Hirjan J, Porterfield JS, Arslanagić E, editors. Arboviruses in the Mediterranean countries. 6th FEMS Symposium. Supetar, Brač, 1978) b. Human infection with Eperythrozoon Puntaric V, Borcic D, Vukelic D, Jeren T, Burek V, Wikerhauser T, Richter B. Eperythrozoonosis in man. Lancet 1986: 2(8511):868-9. c. Human infection with Grahamella Puntaric V, Borcic D, Bejuk D, Vrhovec B, Madic J, Busch K, Richter B. Haemotropic bacteria in man. Lancet 1994;343(8893):359-60. Professor Alemka Markotić, MD, PhD Head, Research Department 2 3 Facts about the University Hospital for Infectious Diseases "Dr. Fran Mihaljević" Zagreb, Croatia www.bfm.hr The University Hospital for Infectious Diseases (UHID) is affiliated with the University of Zagreb Medical School and is the leading institution for the treatment of infectious diseases in Croatia. This tertiary care provider treats over 8,000 inpatients and 65,000 outpatients per year. Most patients are from Zagreb, but many are referred to the UHID from throughout Croatia and neighboring countries. History The University Hospital for Infectious Diseases was founded in the Spring of 1893 as The City Hospital for Contagious Diseases (CHCD). The impetus for the facility was the increase of rapid travel in the late 19th century and the increased the risk for disease transmission associated with the movement of people and goods. For example, the spread of cholera through Europe and the Middle East presented great dangers to Croatia and its neighbors. Gradually, the CHCD grew larger and admitted patients with classical infectious diseases (diphtheria, scarlet fever, typhus exanthematicus, etc.). During the First World War, the CHID had 800 beds, and served as civilian and a military hospital. In 1914, the CHID had a primitive laboratory for the basic analysis of blood and urine. However, thanks to the work of two distinguished clinicians, Dr. Steinhardt and Dr. Fran Mihaljević, the UHID grew rapidly between 1920-30. (The UHID was named after Dr. Mihaljević in 1978). In the 1950s hospital buildings were reconstructed and new buildings and wards were established, including the first intensive care unit in Croatia. The UHID hospital became affiliated with the Medical School of theUniversity of Zagreb in 1956 with the establishment of the Department for Infectious Diseases. This Department has trained undergraduates, postgraduates and medical students for more than fifty years. Laboratory and diagnostic medicine capabilities developed rapidly in the 1970 and now include modern immunologic and molecular medicine procedures. Rapid advances in research programs in many areas of infectious disease have occurred in the past 20 years. 3 4 Management structure Capacity 232 beds (172 for adults, 60 for children) Inpatients – 8,000 /year Outpatients – 65,000 /year Average daily census – 211 (162 adults, 49 children) Average length of stay (days) – 10.52 Catchment Area Approximately 1 million, the population of Zagreb. Croatia has a total population of 4.3 mil. Staff Total Medical Staff MD, specialists in infectious diseases MD, specialists in microbiology, epidemiology, radiology, cytology Residents in infectious diseases PhD students BSc degree (clinical biochemistry, molecular biology, pharmacy, etc.) Medical nurses, technicians Administrative and other non-medical staff Total 4 415 46 Full time 374 46 Part time 41 - 9 9 - 5 14 16 5 14 16 - 329 228 288 228 41 - 643 602 41 5 Medical services Clinical Departments and Divisions Clinical Department for Acute Respiratory Infections Clinical Department for Urinary Tract Infections Clinical Department for Gastrointestinal Infections Clinical Department for Viral Hepatitis Clinical Department for Infections in the Immunocompromised Clinical Department for Adult Intensive Care and Neuroinfections Clinical Department for Pediatric Infectious Diseases Division for Newborns and Infants Division for Young Children with PICU Division for Preschool and School Children Clinical Department for Exanthematous Diseases Clinical Department for General Infectious Diseases Department for Radiology and Ultrasound Diagnostics Emergency Department Research Department Pharmacy Medical Records Department Outpatient departments: Central Admissions Department - Day Hospital – Patients arriving for first examination Polyclinics - Follow-up visits of previously hospitalized patients Reference Centers of The Ministry of Health and Social Welfare Reference Center for Diagnostics and Treatment of Infectious Diseases Reference Center for Diagnostics and Treatment of Viral Hepatitis Reference Center for Diagnostics and Treatment of HIV-infection Reference Center for Urinary Tract Infections Reference Center for Antibiotic Resistance Surveillance Reference Center for Tropical and Traveler Diseases 5 6 Central Diagnostic Laboratory (CDL) New facility constructed and equipped for Clinical Laboratory Diagnostics: Department for Clinical Microbiology Division of Bacteriology and Hospital Infections Division of Virology Division of Parasitology Department of Clinical Immunology Department of Medical Biochemistry Department of Diagnostic Hematology and Cytology Department of Molecular Diagnostics and Flow Cytometry The CDL Facility includes this new Biosafety Level 3 (BSL-3) Laboratory with Cell Culture Unit (Research Department) and BSL-2+ Laboratory for Prion Diseases. 6 7 Educational activities The University Hospital for Infectious Diseases is a teaching hospital for: - Undergraduate and Postgraduate students of Medicine (Department for Infectious Diseases of the University of Zagreb Medical School) - Students of Dental Medicine (Department for Infectious Diseases of the University of Zagreb School of Dental Medicine), - Medical doctors specializing in other medical fields - Clinical Microbiology, Epidemiology, Internal Medicine, Neurology, Pediatrics, etc., (Department for Infectious Diseases of the University of Zagreb Medical School) - Medical nurses and technicians (University of Applied Health Studies, Zagreb, Croatia). Professional Societies of The Croatian Medical Association affiliated with UHID Croatian Society for Infectious Diseases (incl. Section for Respiratory Tract Infections) Croatian Society of Chemotherapy Croatian Society of Urogenital and Sexually Transmitted Infections (incl. Section for Chlamydia trachomatis, Section for Human Papillomavirus) 7 8 Principal investigator Professor Bruno Baršić, MD, PhD Research team Assistant Professor Suzana Bukovski, MD, PhD Dragan Lepur, MD PhD or postdoctoral students Marko Kutleša, MD, Marija Santini, MD, Ana Pangerčić, MD, Vladimir Krajinović, MD Address Intensive Care Unit, University Hospital for Infectious Diseases, Mirogojska 8, Zagreb, Croatia, tel. ++385 91 4012604, fax. ++385 1 2826 255; e-mail: [email protected] Research topics 1. Antimicrobial and other adjunctive therapy of critically ill infectious disease patients a. Hypothermia in patients with bacterial and viral meningoencephalitis Patients with low GCS and reduced CO2 reactivity measured by transcranial doppler are included and subdued to hypothermia (33 0C) for 4 days. Measurements of cerebral blood flow and optic nerve diameter, as well as lactate index are performed daily. Outcome>: Glasgow Outcome Score b. Timing of appropriate antibiotic treatment of ICU acquired blood stream infections Evaluation of outcome of ICU treatment regarding timing of appropriate antimicrobial treatment particularly in patients with nosocomial blood stream infections. c. Are glycopetides still the best choice for the treatment of MRSA infections? Observational study including patients with S. aureus infection. Correlation of outcome with vancomycin MIC values. Comparison of vancomycin with other antibiotics (linezolid, daptomycin). 2. Infective Endocarditis a. Influence of early cardiac surgery on patients’ outcome Observational study in patients with definite IE – comparison of outcome between early surgery groups (within 14 days from diagnosis) with no surgery or postponed surgery. b. Factors associated with development of endocarditis during staphylococcxal blood stream infection Observational study including patients with staphylococcal bacteremia. Comparing patients with endocarditis and no endocarditis regarding cardiac diseases, and permanent use of statins, acetylsalicylic acid, anticoagulants. Ongoing or previous national projects Nosocomial infections in ICU (previous) Therapeutic aspects of care for critically ill patient Ongoing or previous international projects International Collaboration on Endocarditis (ICE) – Duke Medical Research Centre, Durham, NC, Ralph Correy – Principal Investigator EPIC II Study – Erasmus University Brussels – Jean Louis Vincent, Principal Investigator 8 9 Collaboration with international institutions Duke Research Center (ICE Study) Teaching and educational involvement School of Medicine Zagreb (Chair of the Department for Infectious Diseases) International research experience and study visits Mary Hitchcock Memorial Hospital Lebanon, NH Selected publications 1. Santini M, Kutlesa M, Pangercic A, Barsic B. The importance of pathogens in sepsis: Staphylococcus aureus story. Scand J Infect Dis. 2009 [Epub ahead of print] 2. Kutlesa M, Lepur D, Daković-Rode O, Schoenwald N, Barsic B. Severe late-onset nosocomial pneumonia caused by Chlamydophila pneumoniae. Acta Clin Croat 2009 Jun;48(2):179-82. 3. Bae IG, Federspiel JJ, Miró JM, Woods CW, Park L, Rybak MJ, Rude TH, Bradley S, Bukovski S, de la Maria CG, Kanj SS, Korman TM, Marco F, Murdoch DR, Plesiat P, Rodriguez-Creixems M, Reinbott P, Steed L, Tattevin P, Tripodi MF, Newton KL, Corey GR, Fowler VG Jr; International Collaboration on Endocarditis-Microbiology Investigator. Heterogeneous vancomycin-intermediate susceptibility phenotype in bloodstream methicillin-resistant Staphylococcus aureus isolates from an international cohort of patients with infective endocarditis: prevalence, genotype, and clinical significance. J Infect Dis 2009;200(9):1355-66. 4. Lepur D, Barsic B. Incidence of neurological complications in patients with native-valve infective endocarditis and cerebral microembolism: an open cohort study. Scand J Infect Dis. 2009;41(10):708-13. 5. Durante-Mangoni E, Bradley S, Selton-Suty C, Tripodi MF, Barsic B, Bouza E, Cabell CH, Ramos AI, Fowler V Jr, Hoen B, Koneçny P, Moreno A, Murdoch D, Pappas P, Sexton DJ, Spelman D, Tattevin P, Miró JM, van der Meer JT, Utili R; International Collaboration on Endocarditis Prospective Cohort Study Group. Current features of infective endocarditis in elderly patients: results of the International Collaboration on Endocarditis Prospective Cohort Study. Arch Intern Med. 2008;168(19):2095-103. 6. Vince A, Lepej SZ, Barsic B, Dusek D, Mitrović Z, Serventi-Seiwerth R, Labar B. LightCycler SeptiFast assay as a tool for the rapid diagnosis of sepsis in patients during antimicrobial therapy. J Med Microbiol. 2008;57(Pt 10):1306-7. 7. Kutlesa M, Lepur D, Bukovski S, Lepur NK, Barsic B. Listeria monocytogenes meningitis associated with rhabdomyolysis and acute renal failure. Neurocrit Care 2009;10(1):70-2. 8. Barsic B, Beus I, Marton E, Himbele J, Klinar I. Nosocomial infections in critically ill infectious disease patients: results of a 7-year focal surveillance. Infection. 1999;27(1):16-22. 9. Meissner B, Kallenberg K, Sanchez-Juan P, Ramljak S, Krasnianski A, Heinemann U, Eigenbrod S, Gelpi E, Barsic B, Kretzschmar HA, Schulz-Schaeffer WJ, Knauth M, Zerr I. MRI and clinical syndrome in dura mater-related Creutzfeldt-Jakob disease. J Neurol 2009;256(3):355-63. 10. Krajinović V, Tambić Andrasević A, Barsic B. Tricuspidal valve endocarditis due to Yersinia enterocolitica. Infection 2007;35(3):203-5. Other related web-links (personal web-sites, PubMed references, CROSBI or similar) http://tkojetko.irb.hr/znanstvenikDetalji.php?sifznan=2888 9 10 Principal investigator Professor Josip Begovac, MD, PhD Research team Klaudija Višković, MD, PhD, Specialist in Radiology Miroslav Lisić, MD, PhD, Associate Professor of Infectious Diseases Davorka Lukas, MD, PhD, Assistant Professor of Infectious Diseases Drago Turčunov, MD, PhD Šime Zekan, MD PhD or postdoctoral students Kozić Sanja, BSc Djaković-Rode Oktavija, MD Marta Perović, MD Address HIV/AIDS Center, University Hospital for Infectious Diseases, Mirogojska 8, Zagreb, Croatia tel. ++385 1 2826 150; fax. ++385 1 4678 235, e-mail: [email protected] Research topics HIV/AIDS, Lipodystrophy, Dyslipidemia, Mediterranean diet, Streptococcal infections Ongoing or previous national projects Epidemiological, clinical and laboratory features of HIV infection in the era of highly active antiretroviral treatment (HAART) in patients from Croatia Ongoing or previous international projects EUROSIDA Collaboration with international institutions Institute for Global Health, University of San Francisco Teaching and educational involvement Infectious diseases, University of Zagreb Medical School International research experience and study visits 1996 Dartmouth Medical Center, USA 2003 Institute for Global Health, University of San Francisco 10 11 Selected publications 1. Begovac J, Marton E, Lisic M, Beus I, Bozinovic D, Kuzmanovic N. Group A betahemolytic streptococcal toxic shock-like syndrome. Pediatr Infect Dis J 1990;9:369-370. 2. Begovac J, Bobinac E, Benic B, Desnica B, Maretic T, Basnec A, Kuzmanovic N. Asymptomatic pharyngeal carriage of beta-haemolytic streptococci and streptococcal pharyngitis among patients at an urban hospital in Croatia. Eur J Epidemiol 1993;9:405410. 3. Begovac J, Jeren T, Kuzman I, Jukic V, Ravlic Z, Andrasevic S. Health status of 1458 Croatian prisoners of war, 1991-1992. JAMA 1993;270:574-575. 4. Begovac J, Kuzmanovic N, Bejuk D. Comparison of clinical characteristics of group A streptococcal bacteremia in children and adults. Clin Infect Dis 1996;23:97-100. 5. Bace A, Zrnic T, Begovac J, Kuzmanovic N, Culig J. Short-term treatment of pertussis with azithromycin in infants and young children. Eur J Clin Microbiol Infect Dis 1999;18:296298. 6. Schonwald S, Begovac J, Skerk V. Urinary tract infections in HIV disease. Int J Antimicrob Agents 1999;11:309-311. 7. Begovac J, Mihaljevic I, Perrin L. HIV-1 and HCV infections among antibody-negative blood donors. N Engl J Med 2004;351:2232-2235; author reply 2232-2235. 8. Rode OD, Lepej SZ, Begovac J. Low seroprevalence of human herpesvirus 8 infection in Croatia. Clin Infect Dis 2005;40:1208. 9. Begovac J, Zekan A, Skoko-Poljak D. Twenty years of human immunodeficiency virus infection in Croatia-an epidemic that is still in an early stage. Coll Antropol 2006;30 Suppl 2:17-23. 10. Zekan S, Novotny TE, Begovac J. Unsafe sexual behavior among HIV-infected patients in Croatia, 2006: prevalence and associated factors. AIDS Behav 2008;12:S86-92. 11. Turcinov D, Stanley C, Canchola JA, Rutherford GW, Novotny TE, Begovac J. Dyslipidemia and adherence to the Mediterranean diet in Croatian HIV-infected patients during the first year of highly active antiretroviral therapy. Coll Antropol 2009;33:423-430. 12. Turcinov D, Stanley C, Rutherford GW, Novotny TE, Begovac J. Adherence to the Mediterranean diet is associated with a lower risk of body-shape changes in Croatian patients treated with combination antiretroviral therapy. Eur J Epidemiol 2009;24:267-274. 13. Bozicevic I, Rode OD, Lepej SZ, Johnston LG, Stulhofer A, Dominkovic Z, Bacak V, Lukas D, Begovac J. Prevalence of sexually transmitted infections among men who have sex with men in Zagreb, Croatia. AIDS Behav 2009;13:303-309. Other related web-links (personal web-sites, PubMed references, CROSBI or similar) http://bib.irb.hr/lista-radova?autor=143475&period=2007 11 12 Principal investigator Professor Antun Beus MD, PhD Director, UHID Other key personnel From UHID: Boško Desnica, MD Jasminka Granić, DVM, MSc Nina Vicković, MD, MSc Mirjana Balen Topić, MD, MSc Nikola Makek, MD From collaborative institutions_ Professor Dagny Stojčević, DVM, PhD, School of Veterinary Medicine, University of Zagreb Professor Ivan Drinković, MD, PhD, Medical School, University of Zagreb Address Office of Director, Department for gastrointestinal diseases and parasitic diseases University Hospital for Infectious Diseases, Mirogojska 8, Zagreb, Croatia tel. ++385 1 2826 222 fax. ++385 1 4678 235 e-mail: [email protected] Research topics Echinococcosis therapy Parasitic diseases Tropical and travel medicine Ongoing or previous national projects 2006-present Determination of the most effective echinococcosis therapy, funded by The Croatian Ministry of Science, Education and Sport Ongoing or previous international projects 2004-2009- MetaNET-SEE – Netzwerk akademischer Netzwerke, University Bielefeld, Germany International research experience and study visits 1980 – Tropical and travel medicine – Mexico City, Mexico 12 13 Selected publications 1: Markotić A, Skerk V, Cvetko Krajinović L, Beus A. Is the clinical picture of Schnitzler syndrome always Schnitzler syndrome? Clin Exp Rheumatol. 2009;27(3):507-9. 2: Desnica B, Beus A, Skuhala T, Topic MB, Vickovic N, Makek N. Myalgia and swelling of interphalangeal joints as side-effect of prolonged azithromycin therapy in patient with pelvic actinomycosis: case report. Scand J Infect Dis. 2007;39(2):186-7. 3: Topic A, Skerk V, Puntaric A, Milavec Puretic V, Beus A, Begovac J. Azithromycin: 1.0 or 3.0 gram dose in the treatment of patients with asymptomatic urogenital chlamydial infections. J Chemother. 2006 Feb;18(1):115-6. 4: Puljiz I, Beus A, Kuzman I, Seiwerth S. Electrocardiographic changes and myocarditis in trichinellosis: a retrospective study of 154 patients. Ann Trop Med Parasitol. 2005 Jun;99(4):403-11. 5: Skerk V, Schönwald S, Krhen I, Markovinović L, Beus A, Kuzmanović NS, Kruzić V, Vince A. Aetiology of chronic prostatitis. Int J Antimicrob Agents. 2002 Jun;19(6):471-4. 6: Skerk V, Schönwald S, Strapac Z, Beus A, Francetić I, Krhen I, Lesko V, Vuković J. Duration of clinical symptoms in female patients with acute urethral syndrome caused by Chlamydia trachomatis treated with azithromycin or doxycycline. J Chemother. 2001 Apr;13(2):176-81. 7: Skerk V, Schönwald S, Strapac Z, Beus A, Francetić I, Krhen I, Lesko V, Vukovic J. Duration of clinical symptoms in female patients with acute urethral syndrome caused by Ureaplasma urealyticum treated with azithromycin or doxycycline. J Chemother. 2000 Dec;12(6):533-5. 8: Lukas D, Beus A. [Cholera threatens again]. Lijec Vjesn. 1994 Jul-Aug;116(7-8):169-74. Croatian. 9: Beus A. [Comparative study of thiabendazole and mebendazole in strongyloidiasis]. Lijec Vjesn. 1989 Mar;111(3):98-101. Other related web-links (personal web-sites, PubMed references, CROSBI or similar) http://bib.irb.hr/lista-radova?autor=61516&period=2007 13 14 Principal investigator Professor Vitomir Burek, MD, PhD Research team Jadranka Horvat Cvrlje Vesna Banić Marko Grgurević Ivica Professor Ranko Ostojić, MD, PhD Židovec-Lepej, PhD Mikulić Radojka Address Department for Clinical Immunology, University Hospital for Infectious Diseases, Mirogojska 8, 10000 Zagreb, Croatia, tel: ++385 1 2826 162; fax: ++385 1 2826 151 e-mail: [email protected] Research topics Viral hepattis C, B, coinfection B/C in the general population and selected populations in Croatia HIV infection Ongoing or previous national projects Immunologic aspects of viral hepatitis, Ministry of Science Republic of Croatia Transmission of hepatitis B virus from mother asymptomatic carrier of Hepatitis Bs antigen to newborn child, Ministry of Science Republic of Croatia Immunologic characteristics of HIV infected individuals, Ministry of Science Republic of Croatia Ongoing or previous international projects Vertical transmission of hepatitis B virus, consequences and possibility of protection, NIH (National Institutes of Health), Bethesda, USA, 1985-1987, Foreign Research Agreement No. JFP 611 US Department of Health, Education and Welfare, Public Health Service Collaboration with international institutions 1. Max von Pettenkofer Institut, University of Munich, Germany Viral hepatitis Gürtel Gerhard 2. Biomedical Research Center, Immuno, Wiena, Austria HIV infection Gerencer Marijan 14 15 International research experience and study visits Study visit: Poland: Warsaw-Higienic Institute, Infective Clinic: Poznan-Infective Clinic Study visit: London Radioisotopic and Labelled Reagent Microanalytical Methods, Medical School Study visit: Washington/ Bethesda (National Institute of Health – NIH), USA Study visit: Atlanta, Center for Disease Control – CDC, USA Study visit: Viena, Universität Klinik, Viena, Austria Study visit: Paris, 1 eres Recontres Europeennes Est-Quest Toxicomanie, France Study visit: Viena, Universitat Klinik, Viena, Austria Study visit: Salluga, (Research center, Salluga), Italiy. Study visit: Gottingen, George-August-Universitat, Klinik fur Neurologie, CJDForschungsgruppe), Germany Selected publications 1. Burek V, Plavljanić Đ, Šlamberger S, Vitale B. Studies on the mechanism of allogeneic disease in mice. The influence of bone marrow T lymphocytes on the differentiation and proliferation of hemopoietic stem cells. Exp Hemat 1977;5:465-479. 2. Vitale B, Burek V, Plavljanić Đ. Influence of T lymphocytes on the process of differentiation and proliferation of bone marrow cells. Transplant Proc 1977;9:269-272. 3. Burek V, Baće A, Kačić M, Belošević, Mravunac B. Prevalence of antibodies to hepatitis A virus among urban children aged 0 - 7 years in Yugoslavia. J Infect 1985;10:71-75. 4. Burek V, Hudolin V, Sakoman S, Kovač D, Ficović F, Čepelja S, Petričević I, Schonwald S, Soldo I, Borčić D. HTLV-III antibody in Yugoslav members of high risk groups for AIDS. Period Biol 1986;88:68-69. 6. Burek B, Burek V, Košutić Z. Local Xenogeneic Graft-Versus-Host Reaction: A Measure of T-Cell Function in Asymptomatic HBsAg Carriers. J Clin Lab Immunol 1988;27:69-72. 7. Burek V, Mišić-Mayerus LJ, Maretić T. Antibodies to Borrelia burgdorferi in Various Population Groups in Croatia. Scand J Infect Dis 1992;24:683-684. 8. Burek V, Gerenčer M. Inhibition of Classical Complement Activation by Sera From HIV-1 Positive Patients. Clin Immunol Immunopathol 1996;81(2):114-121. 9. Gerenčer M, Burek V, Barett NP, Dorner F. Acquired Eficiency of Functional C1 Esterase Inhibitor in HIV Type 1 – Infected Patients. J AIDS Res Hum Retrovirus 1997;13(10):813-815. 10. Gerenčer M, Burek V, Crowe BA, Barett np, Dorner F. The role of complement and gp 120-specific antibodies in virus lysis and CD4+ T cell depletion in HIV-1-infected patients. Microb Pathogen 1998;25(5):252-266. 11. Burek V, Mikulić R. Reappearance of free HCVAg after immunosuppression with decrease of anti-HCV spectrum. Irish J Med Sci. 2003;172:13. 12. Burek V, Mikulić R. HCV core antigen and the time of HCV infection. Int J Infect Dis. S42, 2004. 13. Gerenčer M, Burek V. Identification of HIV-1 proteaze cleavage site in human C1-inhibitor. Virus Res 2004;105:97-100. 14. Romic Z, Derek L, Burek V, Petrovecki M, Unic A. Anty-cyclic citrullinated peptide and rheumatoid factor in HIV positive patients. J Clin Laborat Anal 2009. (in press) 15. Burek V, Horvat J, Butorac K, Mukulic R. Viral Hepatitis B, C and HIV infection in Croatian prisons. Epidemiology and Infection 2009. (in press) 15 16 Principal investigator (group leader) Professor Ilija Kuzman, MD, PhD Research team Ivan Puljiz, MD,PhD Oktavija Đaković-Rode, MD, MSc Professor Alemka Markotić, MD, PhD Neala Schoenwald, MD, PhD Klaudija Višković, MD, PhD Renata Laškaj, PhD PhD student: Tomislava Skuhala, MD Address Department for Respiratory Tract Infections, University Hospital for Infectious Diseases, Mirogojska 8, 10 000 Zagreb, Croatia, tel: ++385 1 2826 118; fax: ++385 1 4578 235 e-mail: [email protected] Research topics Investigation of the causative pathogens, clinical and laboratory parameters, severity of clinical symptoms and treatment of pneumonia with the development of an algorithm for diagnosis, assessment of disease severity, hospitalization and antibiotic use. This research is a continuation of our previous research project. Novelty in the research of pneumonia is aimed at explaining pathogenic and immunological mechanisms, occurrence and outcome of disease. Therefore, the focus of our investigation is the role and the effects of beta-chemokines and matrix metalloproteinases (MMPs) in the pathogenesis of the disease. - Beta-chemokines and matrix metalloproteinases most probably participate in complicated pathogenic and immunologic mechanisms associated with the development and clinical presentation of pneumonia. Our hypothesis based research aims to determine their roles in the pathologies associated with organisms that frequently cause community-acquired pneumonia (Streptococcus pneumoniae, Mycoplasma pneumoniae, Legionella pneumophila) - In patients with pneumonia we record an elevated level of beta-chemokines (IL-8, MCP-1 and MIP-1β) in systemic circulation - The level of beta-chemokines and matrix metalloproteinases in systemic circulation has a very good correlation with the clinical course of disease . The clinical course of disease is more severe in patients with higher levels of metalloproteinases. - The values of these immunological indicators can be correlated with routine laboratory findings, and also with interrelations Other hypotheses: - The growing resistance of pneumococcus to beta-lactam antibiotics is not today a barrier for the treatment of pneumonia with penicillin and other beta-lactam antibiotics - It is necessary to determine the criteria for severity of disease, hospitalization, when to switch therapy and the duration of pneumonia treatment 16 17 Ongoing or previous national projects Pneumonia – diagnostic and treatment guidelines Teaching and educational involvement Croatia, Medical School, University of Zagreb Bosnia and Herzegovina, University of Mostar, High Medical School, Department of Infectology Selected publications 1. Kuzman I, Soldo I, Schonwald S, Čulig J. Azithromycin for treatment of community acquired pneumonia caused by Legionella pneumophila: A retrospective study. Scand J Infect Dis 1995; 27:503-5. 2. Schonwald S, Kuzman I, Orešković K, Burek V, Škerk V, Car V, Božinović D, Čulig J, Radošević S. Azithromycin: Single 1.5 dose in the treatment of patients with atypical pneumonia syndrome – A randomized study. Infection 1999; 27:198-202. 3. Markotić A, Nichol ST, Kuzman I, Sanchez AJ, Ksiazek TG, Gagro A, Rabatić S, Zgorelec R, Avšič-Županc T, Beus I, Dekaris D. Characteristics of Puumala and Dobrava infections in Croatia. J Med Virol 2002; 66:542-51. 4. Puljiz I, Kuzman I, Markotić A, Turčinov D, Matić M, Makek N. Electrocardiographic changes in patients with haemorrhagic fever with renal syndrome. Scand J Infect Dis 2005; 37:594-8. 5. Kuzman I, Puljiz I, Đaković-Rode O. Cure Q fever pneumonia with moxifloxacin: Case report. Scand J Infect Dis 2005; 37:778-80. 6. Kuzman I, Đaković-Rode O, Oremuš M, Banaszak AM. Clinical efficacy and safety of a short regimen of azithromycin sequential therapy vs standard cefuroxime sequential therapy in the treatnment of community-acquired pneumonia: An international, randomized, open-label study. J Chemother 2005; 17:636-42. 7. Puljiz I, Kuzman I, Đaković-Rode O, Schoenwald N, Miše B. Chlamydia pneumoniae and Mycoplsma pneumoniae: Comparison of clinical, epidemiological characteristics and laboratoty profiles. Epidemiol Infect 2006: 134:548-55. 8. Laškaj R, Dodig S, Čepelak I, Kuzman I. Gamma-glutamyltransferase activity and total antioxidant status in serum and platelets of patients with community-acquired pneumonia. Arch Med Res 2007; 38:424-31. 9. Kosor Krnić E, Gagro A, Draženović V, Kuzman I, Jeren T, Čečuk-Jeličić E, KerhinBrkljačić V, Gjenero-Margan I, Kaić B, Rakušić S, Sabioncello A, Markotić A, Rabatić S, Mlinarić-Galinović G, Ddekaris D. Enumeration of haemagglutinin-specific CD8+ T cells after influenza vaccination using MHC class I peptide tetramers. Scand J Immunol 2007; 67:86-94. 10. Laškaj R, Dodig S, Čepelak I, Kuzman I. Superoxide dismutase, copper and zinc concentrations in platelet-rich plasma of pneumonia patients. Ann Clin Biochem 2009; 46:123-8. Other related web-links (personal web-sites, PubMed references, CROSBI or similar) http://bib.irb.hr/lista-radova?autor=142373&period=2007 17 18 Principal investigator Assistant Professor Davorka Lukas MD, PhD, DTM&H Other key personnel Tomislav Maretić, MD, PhD specialist in infectious diseases Sanja Kozić, BSc Professor Biserka Vukić Trošelj, Department for Infectious Diseases, Clinical Hospital Rijeka PhD students Vanja Romih, MD Teuta Skorin, BSc Address HIV/AIDS Center; Center for Tropical and Travel Medicine; University Hospital for Infectious Diseases, Mirogojska 8, Zagreb, Croatia tel. ++385 1 2826 222 fax. ++385 1 4678 235 e-mail: [email protected] Research topics Tropical and travel medicine, HIV/AIDS, Leishmaniasis (“Leishmaniasis in Balkan countries” project preparations in progress) Ongoing or previous national projects “HIV Infection, Sexual Transmitted Infections and Risky Behavior in Croatian seamen” – principal investigator “Epidemiological, clinical and laboratory features of HIV infection in the era of highly active anitretroviral treatment (HAART) in patients from Croatia” – research team member Ongoing or previous international projects EUROSIDA – research team member Collaboration with international institutions 1. Mahidol – Oxford Tropical Medicine Research Unit, Bangkok, Thailand. Prof Nicholas White 2. WHO, Dr Jorge Alvar, Leishmania control 3. Institute of Public Health, University of California, San Francisco, USA Teaching and educational involvement Infectious Diseases & Tropical Medicine, Medical School, University of Zagreb 18 19 International research experience and study visits 1996 London School of Tropical Medicine and Hygiene (Diploma in Tropical Medicine and Hygiene) 1997 Gambia, MRC Africa (Course: Medicine in Developing Countries) 1999 Karolinska Institute, Department of Immunology, Microbiology, Pathology and Infectious Diseases, Division of Infectious Diseases, Huddinge University Hospital, Sweden (Invited speaker – IMPI Seminar) 2004 Training in Clinical Research Center for AIDS Prevention Studies, University of California, San Francisco, USA (Training in Clinical Research) 2009 Mahidol – Oxford Tropical Medicine Research Unit, Bangkok, Thailand. Prof Nicholas White (Study visit) Selected publications 1. Begovac J, Lukas D. Condylomata Lata of Secondary Syphilis. N Engl J Med 2005;352(7):708. 2. Begovac J, Lisić M, Lukas D, Maretić T, Kniewald T, Novotny T. Marked Improvement in Survival among Adult Croatian AIDS Patients after the Introduction of Highly Active Antiretroviral Treatment. Coll. Antropol 2006;30(1):175-179. 3. Begovac J, Gedike K, Lukas D, Lepej SZ. Late presentation to care for HIV infection in Croatia and the effect of interventions during the Croatian Global Fund Project. AIDS Behav. 2008 Jul;12(4 Suppl):S48-53. Epub 2008 May 6. 4. Bozicevic I, Rode OD, Lepej SZ, Johnston LG, Stulhofer A, Dominkovic Z, Bacak V, Lukas D, Begovac J. Prevalence of Sexually Transmitted Infections Among Men Who Have Sex with Men in Zagreb, Croatia. AIDS Behav. 2008 Aug 9. 5. Maretić T, Perović M, Vince A, Lukas D, Dekumyoy P, Begovac J. meningitis and Radiculomyelitis Caused by Angiostrongylus cantonensis. Emerg Infect Dis. 2009 Jun 6. Other related web-links (personal web-sites, PubMed references, CROSBI or similar) http://bib.irb.hr/lista-radova?autor=232202&period=2007 19 20 Principal investigator Associate Professor Alemka Markotić, MD, PhD Research team From UHID: Oktavija Rode, MD, MSc; Professor Ilija Kuzman, MD; PhD; Professor Višnja Škerk, MD, PhD; Ivan Puljiz, MD, PhD From other Croatian institutions: Professor Josip Margaletić, PhD (Faculty of Forestry, Zagreb); Professor Nenad Turk, DVM, PhD (Faculty of Veterinary Medicine, Zagreb); Marica Miletić Medved, MD, PhD (Public Health Institute of Brodsko-posavska County) PhD students Lidija Cvetko Krajinović, BSc Ivan-Christian Kurolt, BSc Ante Tadin, BSc Dragan Ledina, MD, MSc (Department of Microbiology, Clinical Hospital Split) Address Research Department University Hospital for Infectious Diseases, Mirogojska 8, 10000 Zagreb, Croatia Tel: 385-1-2826-148 Mobile phone: 385-91-4012-648 Fax: 385-1-2826-148 e-mail: [email protected]; [email protected] Research topics Molecular epidemiology of hantaviruses Apoptosis and hantaviruses Epidemiology and risk factors for zoonoses transmission (hantaviruses, borrelia, LCMV, TBE, WNV) Immunoreactions to hantaviruses and leptospires Chronic sequelae and hemorrhagic fever with renal syndrome Immunoreactions, chronic sequelae and zoonoses Emerging and re-emerging infectious diseases Clinical immunology and infectious diseases 20 21 Ongoing or previous national projects • • • • 2002-2004 -3-D technology in the production of human native interferon and recombinant viral proteins; funded by The Croatian Ministry of Science and Technology (P.I. Alemka Markotić) 2002-2005 – Immunoreactions on intracellular pathogens; funded by The Croatian Ministry of Science and Technology (P.I. Alemka Markotić) 2006-present – Immunoreactions to hantaviruses and leptospira, funded by The Croatian Ministry of Science, Education and Sport (P.I. Alemka Markotić) 2007-present – Center for Emerging and Re-emerging infectious Diseases, funded by The Croatian Ministry of Science and Technology (P.I. Alemka Markotić) Ongoing or previous international projects • October 1999-October 2000 – Role of the Cytokines and Chemokines in the Infection of Human Cells with Hantaviruses, National Research Council (NRC) Award, USA; Molecular Virology Department, US Army Medical Research for Infectious Diseases (USAMRIID), Frederick, MD, USA • 2001- Endemic Zoonoses in Croatia and Slovenia with special accent on hemorrhagic fever with renal syndrome (P.I's. Full Member of Academy, Professor Dragan Dekaris, Croatian Academy of Sciences and Arts and Full Member of Academy, Professor Dušan Ferluga, Slovenian Academy of Sciences and Arts) • 2003-2004 - Mechanisms of apoptosis in 293HEK cells infected with hantaviruses, funded by the International Society of Infectious Diseases (ISID)-Small Grants (P.I. Dr. Alemka Markotić) • 2004-2009- MetaNET-SEE – Netzwerk akademischer Netzwerke, University Bielefeld, Germany • 2007 – present –Telemedicine in Emerging Infectious Diseases; University of Texas Medical Branch (UTMB), Galveston, TX, USA 21 22 Collaboration with international institutions CENTER FOR EMERGING AND RE-EMERGING INFECTIOUS DISEASES Emerging infectious disases (EID) are those that have recently appeared in the population, while re-merging diseases (REID) are those that appear again and then spread in the population. Many of the causative agents are dangerous pathogens, usually resistant to existing drugs. Most are microorganisms that could be agents of bioterrorism. The University Hospital for Infectious Diseases "Dr. Fran Mihaljević" together with national and international partners from Europe and USA have organized into a virutual collaborative core-center with the goal of developing a miltidisciplinary approach to EID/REID. The national partners are the Institute of Immunology Zagreb, the Institute of Public Health Brodskoposavska County, Slavonski Brod, the Faculty of Forestry, the Faculty of Veterinarian Medicine and the Croatian Academy of Sciences and Arts, Zagreb. Specific aims of the project are: (1) Diagnostics –The introduction and development of existing and new diagnostic tools for EID/REID. (2) Research – Studies on molecular epidemiology, immunopathogenesis, new therapies and clinical characteristics of EID/REID. (3) Education – Development and organization of national and international courses and conferences on EID/REID topics; training of doctoral studies through existing and new progams; continuous theoretical and practical education for experts and populations at risk in various national and international frameworks and programs. 22 23 Teaching and educational involvement • 1992-1994 – Undergraduate study, Medical Microbiology, Department of Microbiology, Medical School, University of Sarajevo, Bosnia and Herzegovina • 2001- present -Postgraduate Study - Biomedicine, Medical School, University of Zagreb, Croatia- courses: Mechanisms of Allergy Infection of Biomaterials Pathogenesis of infectious diseases • 2008- present –Undergraduate study in English – Infectious Diseases, Medical School, University of Zagreb, Croatia • 2002 – present -Postgraduate Study Biomedicine and Health, Medical School, University of Osijek, Croatia – courses: Pathogenesis of Viral and Bacterial Infections Antimicrobial Therapy • 2002 – present -Undergraduate study, School for Food and Technology, University of Zagreb, Croatia, Lectures in Immunology • 2003- present -Postgraduate study, Biomedicine, Medical School, University of Rijeka, Croatia; Infections caused by hantaviruses • 2010 – Postgraduate study, Biomedicine, Medical School, University of Mostar, Bosnia and Herzegovina; Emerging and re-emerging Infectious Diseases • 2010 – Undergraduate study, University Center for Forensic Sciences, Split, Croatia; Bioterrorism and Biodefense International research experience and study visits October 1999 - October 2000 - National Research Council (NRC), Washington, USA, Research Associatship Programs - Molecular Virology Department, US Army Medical Research for Infectious Diseases (USAMRIID), Frederick, MD, USA 23 24 Selected publications 1. Markotić A, Šarčević A, Hlača D, Barač V, LeDuc JW. Rats and hantavirus disease. Nature Med 1995; 1(10):977. 2. Markotić A, LeDuc JW, Hlača D, Rabatić S, Šarčević A, Dašić G, Gagro A, Kuzman I, Barač V, Avšič-Županc T, Beus I, Dekaris D. Hantaviruses are a likely threat to NATO forces in Bosnia and Herzegovina and Croatia. Nature Med 1996; 2(3):269-270. 3. Markotić A, Dašić G, Gagro A, Sabioncello A, Rabatić S, Kuzman I, Zgorelec R, Smoljan I, Beus I, Avšič Županc T, Dekaris D. Role of the blood mononuclear cell phenotype changes in the pathogenesis of hemorrhagic fever with renal syndrome. Clin Exp Immunol 1999; 115:329-334. 4. Markotić A, Nichol ST, Kuzman I, Sanchez AJ, Ksiazek TG, Gagro A, Rabatić S, Zgorelec R, Avšič Županc T, Beus I, Dekaris D. Characteristics of Puumala and Dobrava Infections in Croatia. J Med Virol 2002; 66:542-551. 5. Markotić A, Kuzman I, Babić K, Gagro A, Nichol S, Ksiazek TG, Rabatić S, Dekaris D. Double trouble: hemorrhagic fever with renal syndrome and leptospirosis. Scand J Infect Dis 2002;34:221-224. 6. Markotić A. The Balkan syndrome. Lancet 2002; 359: 166. 7. Miletić Medved M, Markotić A, Cebalo Lj, Turković B, Avšič Županc T. Haemorrhagic feer with renal syndrome in Croatia. Lancet 2002; 360: 415-416. 8. Markotić A, Hensley L, Geisbert T, Spik K, Schmaljohn C. Hantaviruses induce cytopathic effect and apoptosis in continuous human embryonic kidney cells. J Gen Virol 2003; 84: 2197-2202. 9. Cvetko L, Markotić A, Plyusnina A, Margaletic J, Miletic-Medved M, Turk N, Milas Z, Avsic-Zupanc T, Plyusnin A. Puumala virus in Croatia in the 2002 HFRS outbreak. J Med Virol. 2005 Oct;77(2):290-4. 10. Cvetko L, Turk N, Markotic A, Milas Z, Margaletic J, Miletic-Medved M, Plyusnin A, Baranton G, Postic D, Avsic-Zupanc T. Short report: dual infections with Puumala virus and Leptospira interrogans serovar lora in a bank vole (Clethrionomys glareolus). Am J Trop Med Hyg. 2006 Apr;74(4):612-4. 11. Cebalo Lj, Markotić A. "Chemokine Production Predominates in Human Monocytes Infected with Tula Virus" Viral Immunology. 2007;20(1). 206-13. 12. Markotić A, Hensley L, Daddario K, Spik K, Anderson K, Schmaljohn C. Pathogenic hantaviruses elicit different immunoreactions in THP-1 cells and primary monocytes and induce differentiation of human monocytes to dendritic-like cells. Coll Antropol. 2007;31:1159-67. 13. Markotić A, Skerk V, Cvetko Krajinović L, Beus A. Is the clinical picture of Schnitzler syndrome always Schnitzler syndrome? Clin Exp Rheumatol. 2009;27(3):507-9. Other related web-links (personal web-sites, PubMed references, CROSBI or similar) http://bib.irb.hr/lista-radova?autor=213111&period=2007 http://bib.irb.hr/lista-radova?sif_proj=143-1430115-0103&period=2007 http://www.nzz.hr/index.php?option=com_content&task=view&id=12&Itemid=53 24 25 Principal investigator Professor Višnja Škerk, MD, PhD Research team Associate Professor Alemka Markotić, MD, PhD Professor Ognjen Kraus, MD, PhD and Goran Štimac, MD, PhD, Urology Department, University Hospital “Sestre milosrdnice”, Zagreb Professor Ranka Štern Padovan, MD, PhD, University Hospital “Rebro”, Zagreb PhD students Miram Pasini, MD – enrolled in doctoral studies Martina Vargović, MD - enrolled in doctoral studies Antea Topić, MD, MSc – “Detection of M.genitalium in males with chronic prostatitis syndrome: prevalence, development of diagnostic criteria and determining the needs for routine screening of the population“ (Doctoral dissertation in process) Srđan Roglić, MD - “Comparison of clinical symptoms scored according to the National Institutes of Health chronic prostatitis symptoms index and assessment of antimicrobial treatment in patients with chronic prostatitis syndrome” (Master’ thesis in process) Saša Andrašević, MD, MSc Address Department for Urogenital Tract Infections University Hospital for Infectious Diseases “Dr. Fran Mihaljevic”, Mirogojska 8, 10 000 Zagreb, Croatia, phone: ++385 1 2826 237; fax: ++385 1 2826 471, e-mail: [email protected] Research topics Urogenital infections, sexually transmitted diseases, prostatitis syndrome, chlamydial infections, antmicrobial therapy Ongoing or previous national projects (titles) Principal Investigator in the following research projects of the Croatian Ministry of Science, Education and Sports: Past Clinical and laboratory parameters of bacteremia and sepsis (301-488) Urogenital infections caused by Chlamydia trahomatis (143-004) Clinical significance of Ureaplasma urealyticum and Mycoplasma hominis (143-003) Ongoing Diagnostics and treatment of patients with prostatitis syndrome (143-1080002-0050) Collaboration with international institutions Research on prostatitis syndrome – collaboration with Gianpaolo Perletti, Laboratory of Toxicology and Pharmacology, Università degli Studi dell’Insubria, Busto A./Varese, Italia Vittorio Magri, Urology and Urological Sonography Clinic, Azienda Ospedaliera Istituti Clinici di Perfezionamento, Milano, Italia Kurt G Naber, International Society of Chemotherapy 25 26 Teaching and educational involvement University of Zagreb Faculty of Dental Medicine - Infectious Disease Program University of Zagreb Faculty of Medicine - Infectious Disease Program Selected publications 1. Škerk V, Schönwald S, Strapač Z, Beus A, Francetić I, Krhen I, et al. Duration of clinical symptoms in female patients with acute urethral syndrome caused by chlamydia trachomatis treated with azithromycin or doxycycline. J Chemoth 2001 ;13(2):176-81. 2. Škerk V, Schönwald S, Krhen I, Markovinović L, Baršic B, Mareković I, et al. Comparative analysis of azithromycin and clarithromycin efficacy and tolerability in the treatment of chronic prostatitis caused by chlamydia trachomatis. J Chemoth 2002 ;14(4):384-9. 3. Škerk V, Schönwald S, Krhen I, Markovinovi L, Beus A, Kuzmanovi N-, et al. Aetiology of chronic prostatitis. Int J Antimicrob Agents. 2002 ;19(6):471-4. 4. Škerk V, Schönwald S, Granić J, Krhen I, Baršić B, Mareković I, et al. Chronic prostatitis caused by trichomonas vaginalis - diagnosis and treatment. J Chemoth 2002 ;14(5):537-8. 5. Škerk V, Schönwald S, Krhen I, Banaszak A, Begovac J, Strugar J, et al. Comparative analysis of azithromycin and ciprofloxacin in the treatment of chronic prostatitis caused by chlamydia trachomatis. Int J Antimicrob Agents. 2003 ;21(5):457-62. 6. Škerk V, Čajić V, Markovinović L, Roglić S, Zekan Š, Škerk V, et al. Etiology of chronic prostatitis syndrome in patients treated at the university hospital for infectious diseases »Dr. Fran Mihaljević « from 2003 to 2005. Coll Antropol. 2006 ;30(Suppl 2):145-9. 7. Škerk V, Krhen I, Lisić M, Begovac J, Roglić S, Škerk V, et al. Comparative randomized pilot study of azithromycin and doxycycline efficacy in the treatment of prostate infection caused by chlamydia trachomatis. Int J Antimicrob Agents. 2004 ;24(2):188-91. 8. Škerk V, Krhen I, Schonwald S, Cajic V, Markovinovic L, Roglic S, et al. The role of unusual pathogens in prostatitis syndrome. Int J Antimicrob Agents. 2004 ;24(Suppl 1):S53-6. 9. Škerk V, Markovinović L, Zekan Š, Jakšić J, Lepej SZ, Markotić A, et al. The significance of chlamydia trachomatis in urethritis and prostatitis - differences in therapeutic approach croatian experience. J Chemoth 2009 ;21(1):63-7. 10. Škerk V, Roglić S, Cajić V, Markotić A, Radonić A, Škerk V, et al. Comparison of clinical symptoms scored according to the national institutes of health chronic prostatitis symptoms index and assessment of antimicrobial treatment in patients with chronic prostatitis syndrome. J Chemoth 2009 ;21(2):181-7. Other related web-links (personal web-sites, PubMed references, CROSBI or similar) http://bib.irb.hr/lista-radova?autor=171634&period=2007 26 27 Principal Investigator (Group Leader) Associate Professor Arjana Tambic Andrasevic, MD, PhD Research team Assist. Prof. Suzana Bukovski, MD, PhD Višnja Kovačević, MD, MSc Iva Butić, MD Silvija Šoprek, MD Irina Cazin, MD PhD students Marija Gužvinec, BSc Marko Jelić, BSc Address Department of Clinical Microbiology, University Hospital for Infectious Diseases Mirogojska 8, 10000 Zagreb, Croatia, tel. ++385 1 2826 282; fax ++385 1 2826 280 e-mail: [email protected] Research topics Bacterial resistance to antibiotics Rational antibiotic prescribing Hospital care associated infections Ongoing or previous national projects 1991 – 1995: Croatian Ministry of Health and Technology project "The significance of P-fimbriae detection in choosing therapy for urinary tract infection" (project No. 3-01199 ). 1998 – 2006: Croatian Ministry of Health and Technology project “Principles of antimicrobial agents use” (project No. 108 195). 2007 on: Croatian Ministry of Health and Social Welfare project “Detection and characterization of causative agents in bacterial meningitis and pneumonia” (project No. 143-1080002-0101). 27 28 Ongoing or previous international projects 1996 – 1999 The British Council Academic Links and Interchange Scheme – ALIS project “Comparison of epidemiologically unrelated strains of Staphylococcus aureus that express similar phenotypic characteristics – testing new methods in epidemiological typing”. 2001 - 2004 The British Council Academic Links and Interchange Scheme – ALIS project “Antiobiotic resistance surveillance and detection of resistance mechanisms in bacteria causing human infections”. 2006 - 2008 The MATRA project „Antibiotic resistance surveillance in human medicine“ 2001 – 2009 The European Antibiotic Resistance Surveillance System (EARSS) 2001 – 2010 The European Surveillance of Antibiotic Consumption (ESAC) Collaboration with international institutions United Medical and Dental Schools of Guy’s and St. Thomas’s Hospitals, London, UK two ALIS projects on staphylococcal typing and antibiotic resistance; leaders of the project: A. Tambic Andrasevic from the Croatian side and G.L. French from the British side National Medicines Institute, Warsaw, Poland Research topics: typing of bacterial pathogens; detection of antibiotic resistance mechanisms Contact persons from the Croatian side: A. Tambic Andrasevic, S. Bukovski, M. Guzvinec Contact persons from the Polish side: W. Hryniewicz, M. Gniadkowski, R. Izdebski, J. Fiett Teaching and educational involvement Medical School, Zagreb Medical School, Osijek International research experience and study visits A. Tambic Andrasevic: January 9 – August 8, 1995 "honorary registrar" at Guy's and St. Thomas's Hospital NHS Trust and "lecturer" at the Microbiology department of the United Medical and Dental Schools (UMDS) of Guy's and St. Thomas's Hospitals, London October 14 – November 24, 1996, UMDS Guy's and St. Thomas's November 2 – 16, 1997, Great Ormond Street Hospital for Children August 28 – September 21, 1998, UMDS Guy's and St. Thomas's May 8 – 13, 2006, study visit to the Netherlands June 24 – July 8, 2006, Universitats Klinik Freiburg, Freiburg iBr, Germany M. Guzvinec: May 26-28, 2006: 36 th ESCMID Postgraduate Course on “Detection and characterization of metallo-beta-lactamases”, Verona, Italy April 29 – May 4, 2007: 40th ESCMID Postgraduate Education Course on Bacterial Molecular Typing, Oeiras, Portugal June 15–21, 2008: 7th Workshop “Prof. V. J. Benedi”: Mechanisms of Antimicrobial Resistance. A practical Approach. (ESCMID Postgraduate Education Course), Palma de Mallorca, Spain September 28 – December 11, 2009: short-term FEMS research visit to the Department of Molecular Microbiology, National Medicines Institute in Warsaw, Poland 28 29 Selected publications 1. Tambić A, Power EGM, Tambić T, Snur I, French GL. Epidemiological Analysis of methicillin-resistant Staphylococcus aureus in a Zagreb Trauma Hospital using a randomly amplified polymorphic DNA-typing method. Eur J Clin Microbiol Infect Dis 1999; 18: 335-40. 2. Pankuch GA, Bozdogan B, Nagai K, Tambić Andrašević A, Schoenwald S, Tambić T, Kalenić S, Pleško S, Tepeš N, Kotarski Z, Payerl Pal M, Appelbaum PC. Incidence, epidemiology and characteristics of quinolone-nonsusceptible S. pneumoniae in Croatia. Antimicrob Agents Chemother 2002; 46: 2671-5 3. Tambić Andrašević A, Tambić T, Kalenić S, Janković V. Surveillance for antibiotic resistance in Croatia. Emerg Infect Dis 2002;8:14-18. 4. Nagai K, Appelbaum PC, Davies TA, Kelly LM, Hoellman DB, Tambic Andrasevic A, Drukalska L, Hryniewicz W, Jacobs MR, Kolman J, Miciuleviciene J, Pana M, Setchanova L, Thege MK, Hupkova H, Trupl J, Urbaskova P. Susceptibilities to telithromycin and six other agents and the prevalence of macrolide resistance due to L4 ribosomal protein mutation among 992 Pneumococci from 10 Central and Eastern European Countries. Antimicrob Agents Chemother 2002; 46: 371-7. 5. Malbruny B, Nagai K, Coquemont M, Bozdogan B, Tambic Andrašević A, Hupkova H, Leclercq R, Appelbaum PC. Resistance to macrolides in clinical isolates of Streptococcus pyogenes due to ribosomal mutations. J Antimicrob Ther (JAC), 2002;49:935-9. 6. Barsic B, Tambic A, Santini M, Klinar I, Kutlesa M, Krajinovic V. Antibiotic resistance among nosocomial isolates in a Croatian intensive care unit – results of a twelve-year focal surveillance of nosocomial infections. J Chemother 2004;16:273-281. 7. Giakkoupi P, Tambic-Andrasevic A, Vourli S, Skrlin J, Sestan-Crnek S, Tzouvelekis LS, Vatopoulos AC. Transferable DHA-1 cephalosporinase in Escherichia coli. Int J Antimicrob Agents 2006;27(1):77-80. Epub 2005 Dec 13. 8. Larissa Grigoryan, Flora M. Haaijer-Ruskamp, Johannes G.M. Burgerhof, Reli Mechtler, Reginald Deschepper, Arjana Tambic-Andrasevic, et al. Self-medication with Antimicrobial Drugs in Europe. Emerg Inf Dis 2006;12:452-9. 9. Krzysztori-Russjan J, Tambic Andrasevic A, Bukovski S, Sabat A, Hryniewicz W. First community-acquired methicillin-resistant Staphylococcus aureus (MRSA) strains in Croatia. Clin Micro Inf Dis (CMI) 2006; 12: 695-699. 10. Gužvinec M, Tešović G, Tambić-Andrašević A, Židovec-Lepej S, Trošelj Vukić B, Begovac J. The epidemiology of invasive Streptococcus pneumoniae disease in Croatian children. Med Sci Monit, 2008; 14: PH59-64 Other related web-links (personal web-sites, PubMed references, CROSBI or similar) http://bib.irb.hr/lista-radova?autor=172380&period=2007 29 30 Principal investigator Assistant Professor Goran Tešović, MD, PhD Research team Department for Pediatric Infectious Diseases: Branko MIše, Srđan Roglić , Tihana Kniewald Collaborators from other Departments at the University Hospital for Infectious Diseases: Snježana Židovec Lepej, Arjana Tambić Andrašević, Marija Gužvinec, Božica Sokolić PhD or Postdoctoral Students Anamarija Čavčić, MD Address Department for Pediatric Infectious Diseases, University Hospital for Infectious Diseases “Dr. Fran Mihaljevic”, Mirogojska 8, 10 000 Zagreb, Croatia phone: ++ 385 1 2826 156; fax: ++ 385 1 2826 158, e-mail: [email protected] Research topics • The role of cytokines in the pathogenesis of viral CNS infections in children. Seroepidemiology of invasive pneumococcal disease. • New methods in establishing the true incidence of invasive pneumococcal diseases in childhood • Connatal CMV infection – neurodevelopmental outcome Ongoing or previous national projects Project title: New methods in diagnostics of acute non-bacterial CNS inflammations in children (funded by the Croatian Ministry of Science, Education and Sports, project code 1080000000-3485), project period 2008-2011 Role in the project: Principal Investigator Project title: Neurodevelopmental outcome in children with intrauterine growth retardation and/or hypoxia , funded by the Croatian Ministry of Science, Education and Sports, project code 072-1081870-0025, project period 2007-2009, principal investigator Vlatka Mejaški Bošnjak Role in the project: Investigator 30 31 Teaching and educational involvement (list the programs or Universities) University of Zagreb School of Medicine, Zagreb Study of Medicine, Infectious Diseases Post-graduate studies in infectious diseases, epidemiology and pediatrics (pediatric infectious diseases) Post-graduate PhD studies (pathogenesis of CNS infections) Selected publications 1. Kutlesa M, Tesović G, Knezović I, Mise B, Visković K, Barisić N. Ischemic stroke associated with adenoviral infection in a 4-year old boy. Wien Klin Wochenschr. 2009;121(23-24):776-9. 2. Guzvinec M, Tesović G, Tambić-Andrasević A, Zidovec-Lepej S, Vukić BT, Begovac J. The epidemiology of invasive Streptococcus pneumoniae infections in Croatian children.Med Sci Monit. 2008 Dec;14(12):PH59-64. 3. Tesović G, Poljak M, Lunar MM, Kocjan BJ, Seme K, Vukić BT, Sternak SL, Cajić V, Vince A. Horizontal transmission of the Leningrad-Zagreb mumps strain: A report of three cases. Vaccine. 2008;26(16):1922-5. 4. Lepej SZ, Tesovic G, Sternak SL, Jeren T. Naive and memory CD4+ T-cells in the cerebrospinal fluid of children with aseptic meningitis following measles-mumps-rubella vaccination and enteroviral meningitis. Immunol Invest. 2007;36(3):321-35. 5. Tesović G, Lesnikar V. Aseptic meningitis after vaccination with L-Zagreb mumps strain--virologically confirmed cases. Vaccine. 2006 Sep 29;24(40-41):6371-3. 6. Santak M, Kosutić-Gulija T, Tesović G, Ljubin-Sternak S, Gjenero-Margan I, BeticaRadić L, Forcić D. Mumps virus strains isolated in Croatia in 1998 and 2005: Genotyping and putative antigenic relatedness to vaccine strains. J Med Virol. 2006;78(5):638-43. 7. Forcic D, Ivancic J, Baricevic M, Mahovlic V, Tesovic G, Bozinovic D, Gjenero Margan I, Mazuran R. Genetic characterization of wild type measles virus isolated in Croatia during the 2003-2004 outbreak. J Med Virol. 2005;75(2):307-12. 8. Forčić D, Baricević M, Zgorelec R, Kruzić V, Kaić B, Marina BM, Sojat LC, Tesović G, Mazuran R. Detection and characterization of measles virus strains in cases of subacute sclerosing panencephalitis in Croatia. Virus Res. 2004;99(1):51-6. 9. Vukelic D, Bozinovic D, Morovic M, Tesovic G, Ruzic Sabljic E, Barisic N, Knezovic I. Opsoclonus-myoclonus syndrome in a child with neuroborreliosis. J Infect. 2000 Mar;40(2):189-91. 10. Tesović G, Begovac J, Baće A. Tesović G, Begovac J, Baće A. Aseptic meningitis after measles, mumps, and rubella vaccine. Lancet. 1993 Jun 12;341(8859):1541. Other related web-links (personal web-sites, PubMed references, CROSBI or similar) CROSBI: http://bib.irb.hr/lista-radova?sif_proj=108-0000000-3485&print=true 31 32 Principal investigator Professor Adriana Vince, MD, PhD Research team Department of Viral Hepatitis: Ivan Kurelac, MD, PhD Vjeran Čajić, MD Collaborators from other Departments at the University Hospital for Infectious Diseases: Snježana Židovec Lepej, Sanja Kozic, Bruno Baršić, Josip Begovac, Tomislav Maretić PhD students Davorka Dušek, MD Jelena Budimir, MD Neven Papić, MD Address University Hospital for Infectious Diseases “Dr. Fran Mihaljevic”, Mirogojska 8, 10 000 Zagreb, Croatia, tel. ++ 385 1 2826 403; fax: ++ 385 1 2826 131, e-mail: [email protected] Croatian Reference Center for Viral Hepatitis at the University Hospital for Infectious Diseases, Zagreb consists of: Outpatient Clinics for Viral Hepatitis (3800 patient visits per year) Department of Viral Hepatitis (50 hospitalised patients with hepatitis B and 250 patients with hepatitis C per year) Laboratory for serological diagnostics of viral hepatitis Department of cellular immunity and molecular diagnostics External collaborators of the Reference center: Liver transplantation unit, Clinical Hospital Merkur, Zagreb National centers for IVDU treatment and care Department of molecular genetics, Faculty of Natural Sciences and Mathematics, University of Zagreb NGO HEPATOS, NGO ABC Hepatitis 32 33 Research topics • Seroepidemiology and molecular epidemiology of viral hepatitis • Immunopathogenesis of hepatitis B and C • Clinical research with emphasis on treatment of hepatitis C • Infections in immunocommpromised hosts (transplant patients, hemato-oncology patients), human papillomaviruses, molecular diagnostics of viral infections Ongoing or previous national projects Project title: Immunopathogenesis of hepatitis B and C, funded by the Croatian Ministry of Science, Education and Sports, project code 143-0000000-0117, project period 2007-2009 Role in the project for A. Vince: Principal Investigator Project title: Leukemia and stem cell transplantation, funded by the Croatian Ministry of Science, Education and Sports, project code 108-1081872-1913, project period 2007-2009, principal investigator: B. Labar Role in the project for A. Vince: Investigator Project title: Molecular diagnostics of EBV and CMV infections, funded by the Croatian Ministry of Science, Education and Sports, project code 0143001, project period 2002-2006 Role in the project for A. Vince: Principal Investigator Ongoing or previous international projects 1. Project title: WHO GRASP Prediction of clinical rules in diagnosis of streptoccocal pharyngits in children, project duration 2001-2003, Role in the project for A. Vince: National Coordinator 2. Project title: Optimization of methods for HCV RNA isolation from the blood, bilateral projects between Croatia and Austria, funded by the Croatian Ministry of Science, Education and Sports and Austrian Ministry of Science, project duration 2002-2006 principal investigator for Slovenia: H. Kessler principal investigator for Croatia: A. Vince Collaboration with international institutions Collaborations with other international institutions: 1. Department of Gastroenterology and Hepatology, Medical Faculty Hannover, Prof. Heiner Wedemeyer 2. Laboratory for Molecular Virology and Slovenian Reference center for AIDS, Medical Faculty, University of Ljubljana, Slovenia, Prof. Mario Poljak 3. Institute of Hygiene, Karl Franz Medical School, Graz, Austria, Prof. Harald Kessler 4. Department of Epidemiology, UCLA School of Public Health, Los Angeles, Anne W. Rimoin, Ph.D., M.P.H, Director, UCLA DRC Research Program 5. EASL member (European Association for Study of Liver) 6. UEMS ID secretary/treasurer (European Assoc.of Medical Specialties-ID section) 33 34 Teaching and educational involvement University of Zagreb School of Medicine, Zagreb, Croatia Graduate and post-graduate studies in infectious diseases (infectious diseases diagnostics, viral hepatitis, cytology,) and Medical studies in English Faculty of Veterinary Medicine, University of Zagreb University of Ljubljana, Slovenia; University of Sarajevo, Bosnia International research experience and study visits 1996-Dartmouth Medical School, NH 1997 Roche Diagnostics Centre, Branchburg 1998, 2001, 2003, 2004 - Graz, Austria, Karl-Franz University 2007-CDC , Atlanta Selected publications 1. Seme K, Lepej SZ, Lunar MM, Iscic-Bes J, Planinic A, Kocjan BJ, Vince A, Poljak M. Digene HPV Genotyping RH Test RUO: comparative evaluation with INNO-LiPA HPV Genotyping Extra test for detection of 18 high risk and probable high risk human papillomavirus genotypes. J Clin Virol 2009;46:176-9. 2. Cavlek TV, Margan IG, Lepej SZ, Kolaric B, Vince A. Seroprevalence, risk factors and hepatitis C virus genotypes in groups with high-risk sexual behavior in Croatia. J Med Virol 2009;81:1348-53. 3. Poljak M, Kocjan BJ, Kovanda A, Lunar MM, Židovec Lepej S, Planinic A, Seme K, Vince A. Human papillomavirus genotype specificity of hybrid capture 2 low-risk probe coctail. J Clin Microbiol 2009;47:2611-5 4. Deterding K, Constantinescu I, Nedelcu FD, Gervain J, Nemecek V, Srtunecky O, Vince A, et al. Prevalence of HBV genotypes in Central and Eastern Europe. J Med Virol 2008;80: 1707-11. 5. Rimoin AW, Walker CL, Chitale RA, Hamza HS, Vince A, et al. Variation in clinical presentation of childhood group A streptococcal pharyngitis in four countries. J Trop Pediatr 2008;54:308-312. 6. Tešović G, Poljak M, Lunar MM, Kocjan BJ, Seme K, Vukić BT, Sternak SL, Čajić V, Vince A. Horizontal transmission o the Leningrad-Zagreb mumps vaccine strain. A report of three cases. Vaccine 2008; 26:1922-5. 7. Vince A, Lepej SZ, Kurelac I, Barsic B, Kozic S, Klinar I, Zarkovic K. Virological and immunological characteristics of fatal Epstein-Barr mononucleosis in a 17-year-old Caucasian male presenting with meningoencephalitis and hemophagocytic syndrome. J Neurovirol 2007;13:389-396. 8. Židovec Lepej S, Grgić I, Poljak M, Iščić-Beš J, Škerk V, Vince D, Dušek D, Vince A. Detection of human papillomavirus genotypes 16/18/45 by hybrid capture hybridisation genotyping probe in clinical specimens: the first report. J Clin Virol 2007;40:171-172. 9. Lepej Židovec S, Mišić.Majerus L, Jeren T, Rode OD, Remenar A, Šporec V, Vince A. Chemokines CXCL10 and CXCL11 in the cerebrospinal fluid of patients with tick-borne encephalitis. Acta Neurol Scand 2007;115:109-114. 10. Vince A, Iščić-Beš J, Židovec Lepej S, Baća-Vrakela I, Bradarić N, Kurelac I, Vince DB. Distribution of hepatitis C virus genotypes in Croatia: a 10 year retrospective study of four geographic regions. Coll Antropol 2006; 30: 139-143. Other related web-links (personal web-sites, PubMed references, CROSBI or similar) http://bib.irb.hr/lista-radova?sif_proj=143-0000000-0117&period=2007 34 35 Principal investigator Snježana Židovec Lepej, PhD, Senior Scientific Associate Research team Department of Cellular Immunology and Molecular Diagnostics staff: Ivana Baca Vrakela, BSc; Ivana Grgic, BSc; Janja Iscic Bes, BSc; Natasa Kutela, BSc; Jasna Matanovic Collaborators from other Departments at the University Hospital for Infectious Diseases: Josip Begovac, Adriana Vince, Goran Tešović, Bruno Baršić, Oktavija Đaković Rode PhD students Ana Planinić, BSc Lana Gorenec, BSc Address Department of Cellular Immunology and Molecular Diagnostics, University Hospital for Infectious Diseases “Dr. Fran Mihaljevic”, Mirogojska 8, 10 000 Zagreb, Croatia tel: ++ 385 1 2826 270; fax: ++ 385 1 2826 131, e-mail: [email protected] Research topics Immunology of infectious diseases, cytokines, antigen-specific immunity, HIV, molecular virology, molecular diagnostics 35 36 Ongoing or previous national projects Project title: Immune reconstitution and resistance to antiretroviral drugs in HIV-infected persons from Croatia (funded by the Croatian Ministry of Science, Education and Sports, project code 143-1080116-0097), project period 2007-2012 Role in the project for S. Zidovec Lepej: principal investigator Project title: Immunopathogenesis of hepatitis B and C, funded by the Croatian Ministry of Science, Education and Sports, project code 143-0000000-0117, project period 2007-2009, principal investigator Adriana Vince Role in the project for S. Zidovec Lepej: investigator Project title: Cellular immunity in tick-borne encephalitis, funded by the Croatian Ministry of Science, Education and Sports, project code 0143999, project period 2003-2006, principal investigator Tatjana Jeren Role in the project for S. Zidovec Lepej: investigator Project title: Characteristics of HIV-infection in Croatia at the time of HAART, funded by the Croatian Ministry of Science, Education and Sports, project code 108026, project period 2001-2006, principal investigator Josip Begovac Role in the project for S. Zidovec Lepej: investigator Project title: Mechanisms of immune reactions and immunomodulation, funded by the Croatian Ministry of Science, Education and Sports, project code 3-01-127, project period 1993-1995, principal investigators Dragan Dekaris and Vlatko Silobrčić Role in the project for S. Zidovec Lepej: investigator Ongoing or previous international projects 1. Project title: EuropeHIVResistance (http://www.europehivresistance.org), funded by the European Union, 6th framework, pribcipal investigators Charles Boucher and Annemarie Wensing, project duration 2003-2009, extention 2010-01-21 Role in the project for S. Zidovec Lepej: National Coordinator 2. Project title: Molecular epidemiology of HIV and resistance to antiretroviral drugs, bilateral projects between Croatia and Slovenia, funded by the Croatian Ministry of Science, Education and Sports and Slovenian Ministry of Science, project duration 2009-2011 principal investigator for Slovenia: Mario Poljak principal investigator for Croatia: S. Zidovec Lepej Collaborations with international institutions 1. Collaborator Ivana Bozicevic, WHO Collaborative Center for Capacity building in HIV Surveillance, Andrija Stampar School of Public Health, Medical School, University of Zagreb, Croatia, http://www.surveillancezagreb.org - educational activities focusing on immunological and virological aspects of HIV infection 36 37 Teaching and educational involvement 1. Faculty of Natural Science and Mathematics, University of Zagreb, Croatia - Graduate studies in molecular biology (molecular techniques in diagnostics) - Post-graduate studies in biomedicine (immunology, cytokines, antiviral immunity) 2. University of Zagreb School of Medicine, Zagreb, Croatia Post-graduate studies in infectious diseases (infectious diseases diagnostics, viral hepatitis, cytology) Selected publications 1. Seme K, Lepej SZ, Lunar MM, Iscic-Bes J, Planinic A, Kocjan BJ, Vince A, Poljak M. Digene HPV Genotyping RH Test RUO: comparative evaluation with INNO-LiPA HPV Genotyping Extra test for detection of 18 high risk and probable high risk human papillomavirus genotypes. J Clin Virol 2009;46:176-9. 2. Cavlek TV, Margan IG, Lepej SZ, Kolaric B, Vince A. Seroprevalence, risk factors and hepatitis C virus genotypes in groups with high-risk sexual behavior in Croatia. J Med Virol 2009;81:1348-53. 3. Poljak M, Kocjan BJ, Kovanda A, Lunar MM, Židovec Lepej S, Planinic A, Seme K, Vince A. Human papillomavirus genotype specificity of hybrid capture 2 low-risk probe coctail. J Clin Microbiol 2009;47:2611-5 4. Božičević I, Đaković Rode O, Židovec Lepej S, Johnston LG, Stulhofer, A, Dominiković Z, Bačak V, Lukas D, Begovac D. Prevalence of sexually-transmitted infections and sexual risk behaviours aomong men who have sex with men in Zagreb, Croatia. AIDS Behav 2009;13:303-9 5. Begovac J, Gedike K, Lukas D, Židovec Lepej S. Late presentation to care for HIV infection in Croatia and the effect of interventions during the Croatian Global Fund Project. AIDS Behav 2008:12(suppl 4): S48-S53. 6. Lepej Židovec S, Mišić.Majerus L, Jeren T, Rode OD, Remenar A, Šporec V, Vince A. Chemokines CXCL10 and CXCL11 in the cerebrospinal fluid of patients with tick-borne encephalitis. Acta Neurol Scand 2007;115:109-114. 7. Židovec Lepej S, Grgić I, Poljak M, Iščić-Beš J, Škerk V, Vince D, Dušek D, Vince A. Detection of human papillomavirus genotypes 16/18/45 by hybrid capture hybridisation genotyping probe in clinical specimens: the first report. J Clin Virol 2007;40:171-172. 8. Đikić D, Židovec Lepej S, Remenar A, Horvat-Knežević A, Benković V, Lisičić D, Sajli L, Springer O. The effects of prometryne on subchronically treated mice evaluated by single cell gel electroforesis (COMET) assay. Environmental Toxicology and Pharmacology. 2008;60:3543. 9. Lepej Židovec S, Mišić-Majerus L, Jeren T, Rode OD, Remenar A, Šporec V, Vince A. Chemokines CXCL10 and CXCL11 in the cerebrospinal fluid of patients with tick-borne encephalitis. Acta Neurol Scand 2007;115:109-114 10. Židovec Lepej S, Grgić I, Poljak M, Iščić-Beš J, Škerk V, Vince DB, Dušek D, Vince A. Detection of human papillomavirus genotypes 16/18/45 by hybrid capture hybridisation genotyping probe in clinical specimens: the first report. J Clin Virol 2007;40:171-2. Other related web-links (personal web-sites, PubMed references, CROSBI or similar) CROSBI: http://bib.irb.hr/lista-radova?sif_proj=143-1080116-0097&period=2007 37
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