MORPHOLOGICAL SPECTRUM OF PROSTATIC LESIONS – A CLINICOPATHOLOGICAL STUDY By Dr. ANUSHREE C. N. Dissertation Submitted to the Rajiv Gandhi University of Health Sciences, Bangalore, Karnataka in partial fulfillment of the requirements for the degree of M. D. (PATHOLOGY) Under the guidance of Dr. KUSUMA V. M. D., D. C. P. DEPARTMENT OF PATHOLOGY KEMPEGOWDA INSTITUTE OF MEDICAL SCIENCES BANGALORE. 2006 i RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES DECLARATION BY THE CANDIDATE I hereby declare that this dissertation entitled “MORPHOLOGICAL SPECTRUM OF PROSTATIC LESIONS - A CLINICOPATHOLOGICAL STUDY” is a bonafide and genuine research work carried out by me under the guidance of Dr. KUSUMA V. M. D., D. C. P, Professor, Department of Pathology, Kempegowda Institute of Medical Sciences, Bangalore. Date: Signature of the Candidate Place: Name: Dr. ANUSHREE C. N. ii CERTIFICATE BY THE GUIDE This is to certify that this dissertation entitled “MORPHOLOGICAL SPECTRUM OF PROSTATIC LESIONS - A CLINICOPATHOLOGICAL STUDY” is a bonafide work done by Dr. ANUSHREE C. N. in partial fulfillment of the requirement for the degree of M. D. in Pathology. Date: Place: Signature of the Guide Dr. KUSUMA V. M. D., D. C. P Professor Department of Pathology Kempegowda Institute of Medical Sciences Bangalore – 560 004 iii ENDORSEMENT BY THE HEAD OF THE DEPARTMENT/PRINCIPAL/HEAD OF THE INSTITUTION This is to certify that this dissertation entitled “MORPHOLOGICAL SPECTRUM OF PROSTATIC LESIONS - A CLINICOPATHOLOGICAL STUDY” is a bonafide work done by Dr. ANUSHREE C. N. under overall guidance of Dr. KUSUMA V M. D., D. C. P, Professor, Department of Pathology, Kempegowda Institute of Medical Sciences. Seal and Signature of the HOD Dr. GEETHAMANI V. M. D. Seal and Signature of the Principal Dr. M. K. SUDARSHAN M. D. Professor and Head Department of Pathology Kempegowda Institute of Medical Sciences Bangalore – 560 004. Principal Kempegowda Institute of Medical Sciences Bangalore – 560 004. Date: Date: Place: Place: iv COPYRIGHT DECLARATION BY THE CANDIDATE I hereby declare that the Rajiv Gandhi University of Health Sciences, Karnataka shall have the rights to preserve, use and disseminate this dissertation / thesis in print or electronic format for academic / research purpose. Date: Signature of the Candidate Place: Name: Dr. ANUSHREE C. N. © Rajiv Gandhi University of Health Sciences, Karnataka v ACKNOWLEDGEMENT Thanksgiving is a pleasant job but it is nonetheless difficult where one sincerely tries to put it in words. These humble words of expression and gratitude cannot wholly convey my feelings. With a deep sense of gratitude, I owe my sincere thanks to my teacher and guide Dr. KUSUMA V. M. D. D. C. P., Professor, Department of Pathology, Kempegowda Institute of Medical Sciences, Bangalore for her able guidance, critical evaluation and constant encouragement, which aided in the timely completion of this dissertation. I am extremely thankful for her guidance and support rendered. It is a great privilege and opportunity to express my heartfelt gratitude and reverence to my teacher and mentor, Dr. GEETHAMANI M D., Professor and Head, Department of Pathology, Kempegowda Institute of Medical Sciences, Bangalore for being the guiding force all the way through this course. I wish to profoundly thank my respected Professors of Pathology Dr. Suguna B. V. M. D. and Dr. Padmavathi K. L. M. D., for their help and finely suggestion. I sincerely thank Associate Professors Dr. Rangaswamy M.D., DNB and Dr. Niveditha S. R. M. D., DNB, for their encouragement and support. I sincerely thank Dr. Hemalatha M. M. D., Dr. Savithri R., D. C. P, DNB, Dr. Preetha D. Prabhu D. C. P., Dr. Gayathri B. R. D. C. P., Dr. Venkatesh Prasad V. D. C. P., Dr. Suja Ajoy Kumar M. D., DNB, for their encouragement and support. I extend my heartfelt gratitude to Dr. M. K. SUDARSHAN M. D., Principal, Kempegowda Institute of Medical Sciences, Bangalore for his beneficial support. vi I wish to record my sincere thanks to Dr. Madhusudhan H. R., Honorary Assistant Professor, Department of Urology, Kempegowda Institute of Medical Sciences, Bangalore for his cooperation towards the progress of this dissertation. I wish to thank Dr. Krishnamurthy former Head, Department of Pathology, Kempegowda Institute of Medical Sciences, Bangalore and Dr. Swarna B., Vydehi Institute of Medical Sciences, Bangalore for their kind support I wish to thank my friend Dr. Sruthi Prasad, for her constant assistance, support and encouragement. My thanks are also due to my other colleagues, technical staff for their help and cooperation. I express my sincere thanks to Shri. K. P. Suresh, Scientist (Statistics), for his assistance extended to me in statistical analysis. I would like to thank Dr. Babu and Mr. A. S. Madhukeshwara, who deserve the credit for the presentation of this manuscript in the present. I am extremely grateful to my Father Chikkanarasimhaiah T. G., B. A., LLB, my Mother Leelavathi D. P., Sister, Brother-in-law and my Niece Krisha M., for encouragement, moral support, friendly advice, which helped me, tide over occasional moments of distress. The completion of this study would not have been possible without their constant encouragement and help. My heartfelt gratitude to all my patients who submitted themselves most gracefully and wholeheartedly participated in this study. Date: Signature of the Candidate Place: Name: vii LIST OF ABBREVIATIONS USED ASAP → Atypical small acinar proliferation BCG → Bacille Calmette Guerin CCCH → Clear Cell Cribriform Hyperplasia DTH → Dihydrotestosterone H&E → Haematoxylin and Eosin HGPIN → High Grade Prostatic Intraepithelial Neoplasia LGPIN → Low Grade Prostatic Intraepithelial Neoplasia NH → Nodular Hyperplasia PIN → Prostatic Intraepithelial Neoplasia PSA → Prostate Specific Antigen SCC → Squamous Cell Carcinoma TCC → Transitional Cell Carcinoma TRUS → Transrectal ultrasound TURP → Transurethral Resection of Prostate WHO → World Health Organization viii ABSTRACT BACKGROUND AND OBJECTIVES Study of prostatic lesions has gained more importance because, development of prostatic hyperplasia is an almost universal phenomenon in aging men. Inflammatory conditions needs correct diagnosis as it can be treated with antibiotics Prostatic carcinoma is the second most common cause of death in men over the age of 55 years. METHODS The study included prostatic tissue specimens received in the pathology laboratory at Kempegowda Institute of Medical Sciences and Hospital, Bangalore from December 2003 to December 2005. A total of 150 specimens were studied. They were graded into benign, inflammatory conditions and malignant lesions. Gleason`s microscopic grading was used to grade all the prostatic adenocarcinoma cases. Serum PSA levels were correlated in all the cases. RESULTS There were 134 TURP, 12 needle biopsies and 4 prostatectomy specimens. Most of the patients were in sixth and seventh decade. Lesions encountered were nodular hyperplasia / prostatitis 90%, HGPIN 0.7% and 9.3% malignant. ix INTERPRETATION AND CONCLUSION Nodular hyperplasia was the common lesion encountered. Associated lesions like prostatitis BCH, CCCH, transitional metaplasia, granulomatous prostatitis, abscesses were encountered. Among the malignant lesions adenocarcinoma was the common lesion seen in seventh decade and Gleason`s score 7 was the commonest. An interesting case of primary squamous cell carcinoma was encountered. One of the case sent as metastatic TCC from bladder was confirmed histologically. Immunohistochemistry for LCA was done in one case, which showed artifactual change of lymphocytes in stroma appearing as signet ring cells. These cells showed positive membrane reaction. Serum PSA levels were elevated in few cases of benign lesions due to associated lesions like prostatitis, abscesses and granulomatous prostatitis. Many of the malignant cases showed very high levels of PSA. KEYWORDS Abscess; Adenocarcinoma of prostate; Gleason`s score; Granulomatous prostatitis; Leucocyte common antigen; Nodular hyperplasia; Prostate specific antigen; Squamous cell carcinoma; Transitional cell carcinoma, Transurethral resection prostate. x TABLE OF CONTENTS Page Nos. 1. INTRODUCTION 1 2. AIMS AND OBJECTIVES 3 3. REVIEW OF LITERATURE 4 4. METHODOLOGY 45 5. RESULTS 47 6. DISCUSSION 71 7. CONCLUSION 83 8. SUMMARY 86 9. BIBLIOGRAPHY 88 10. ANNEXURES • CASE PROFORMA 102 • PROCEDURE FOR SPECIAL STAINS 103 • MASTER CHART 105 • KEY TO MASTER CHART 110 xi LIST OF TABLES Page Nos. 1. Age incidence of the various prostatic lesions 47 2. Clinical presentation of prostatic lesions 49 3. Digital rectal examination findings 51 4. Serum PSA levels in the cases studied 52 5. Clinical Diagnoses in the cases studied 53 6. Nature of prostatic tissue studied 54 7. Microscopic findings in benign lesions studied 55 8. Microscopic findings in malignant lesions studied 57 9. Incidence of carcinoma with reference to Gleason’s score (maximum Score 10) 59 10. Final histopathological diagnosis in the cases studied 60 11. Showing the incidence of prostatitis 72 12. Incidence of PIN in cases of prostates with and without prostatic carcinoma 75 13. Incidence of HGPIN in prostates with carcinoma 75 14. Age incidence of prostatic carcinoma in different studies 77 15. Prevalence of prostatic carcinoma in different studies 78 16. Comparative incidence of carcinoma with reference to Gleason’s score 79 17. Incidence of prostatic carcinoma mean age and mean Gleaso’s score 80 18. Incidence of prostatic carcinoma, median age and Gleason’s score 80 xii LIST OF GRAPHS Page Nos. 1 a.. Age incidence of the various prostatic lesions 48 1 b. Incidence of benign and malignant lesions 48 2 Clinical presentation of prostatic lesions 50 3 Digital rectal examination findings 51 4. Serum PSA levels in the cases studied 52 5. Clinical Diagnoses in the cases studied 53 6. Nature of prostatic tissue studied 54 7. Microscopic findings in benign lesions studied 56 8. Microscopic findings in malignant lesions studied 58 9. Incidence of carcinoma with reference to Gleason’s score (maximum Score 10) 59 10. Final histopathological diagnosis in the cases studied 60 xiii LIST OF FIGURES Page Nos. 1. Gross appearance of nodular hyperplasia (a) Showing hyperplastic lateral lobes (b) Cut section showing solid and cystic areas 61 2. Nodular hyperplasia showing hyperplastic glandular and stromal components (H & E, 100x) 61 3. Basal cell hyperplasia, showing glands filled with small darkly staining basal cells with peripheral palisading (H&E, 100x) 62 4. Clear cell cribriform hyperplasia, showing glands composed of cells with abundant clear cytoplasm (H&E, 100x) 62 5. Transitional metaplasia in a case of nodular hyperplasia (H&E, 100x) 63 6. Squamous metaplasia in a case of nodular hyperplasia (H&E, 100x) 63 7. Prostatic abscess showing sheets of neutrophils in and around the acini (H&E, 100x) 64 8. Chronic prostatitis showing infiltration of lymphocytes, plasma cells and histiocytes in the stroma (H&E, 100x) 64 9. Granulomatous prostatitis showing well formed epithelioid granuloma with a giant cell (H&E, 40x) 65 10 Low grade PIN showing epithelial crowing and stratification with anisonucleosis (a) (H&E, 100X) (b) (H&E, 400X) 65 11. Artifactual change of lymphocytes in the stroma appearing as signet ring cells (H&E, 400x) 66 12. Immunohistochemistry of leucocyte common antigen for the above case showing membrane positivity (a) IHC, 40x, (b) IHC, 400x 66 13. High grade PIN showing pronounced epithelial crowding, stratification, nuclear enlargement with prominent nucleoli and intact basement membrane a) (H&E, 100X), (b) (H&E, 400X) 67 Prostatic adenocarcinoma, Gleason’s score 3+4=7/10 (a) Pattern 3 showing closely packed single glands (b) pattern 4 showing fused glandular pattern (H&E, 100x) 67 15. Prostatic adenocarcinoma, Gleason’s score 4+4=8/10, cribriform gland with irregular border (H&E, 100x) 68 16. Gleason pattern 5 with central comedo necrosis (H&E, 100x) 14. xiv Showing 68 17. Perineural Invasion showing tumour cells invading the perineurium (H&E, 100X) 69 18. Intraluminal acidic mucin in lumens of neoplastic glands in a case of prostatic adenocarcinoma (Alcian blue pH 1, 100x) 69 19. Primary squamous cell carcinoma of prostate (H&E, 100x) inset showing the same (H&E, 400X) 70 20. Transitional cell carcinoma of bladder metastatic to prostate (H&E, 100x) 70 xv INTRODUCTION “When hair becomes gray and scanty, when specks of earthy matter begin to be deposited in tunics of artery, and when a white zone is formed at the margin of the cornea, at this same period the prostate gland usually, might perhaps say invariably becomes increased in size.” - Sir Benjamin Brodie Prostate gland occupies center stage in the lives of many elderly males. Because of its location at bladder neck, enlargement of the gland leads to problems related to urinary obstruction.1 Incidence of prostatic disease, Nodular Hyperplasia (NH) and carcinoma increases with age. Recently there has been a significant advance in understanding of various prostatic diseases. Prostatitis, NH and tumors are the three important lesions to be studied in detail as they are frequently encountered. Diagnosis of prostatitis is very necessary as they can be successfully treated with antibiotics. NH describes a hyper plastic process of stromal and epithelial elements of prostate. It is an extremely common problem in elderly men over the age 50.1 Prostatic carcinoma is more common in India compared to other Asian Countries.2 It is the 5th cause of cancer in men and 4th in cancer mortality in India. At some time in their lives approximately one in 22 Indian males will be struck by prostatic carcinoma and its incidence is increasing by 3.5% every year.3 Etiology of prostatic carcinoma is largely unknown today rendering disease prevention difficult. Hereditary factors have a role.4,5 The great differences in the incidence of clinically manifest 1 carcinoma indicate that the nutritional and environmental factors also may have an influence on the development and progression of the disease.6 Diet rich in animal fats, red meat and diet poor in fruits, vegetables are the likely culprit in prostatic carcinoma.7 Low levels of dietary selenium, vitamin E and vitamin D also play a role.8 Studies also reveal that there is no definite role of sexual activity, smoking, height, weight and alcohol consumption.9 Although nodular hyperplasia can almost be considered as an aging process, the histological variations like different types of hyperplasias, low grade prostatic intraepithelial neoplasia (LGPIN) and high grade prostatic intraepithelial neoplasia (HGPIN) merits discussion. Prostatic carcinoma also has to be given importance as its incidence is increasing owing to the westernization in Asian countries including India. This study comprises description of incidence of various lesions of prostate encountered at KIMS hospital, associated clinical manifestations, morphological changes and also serum Prostatic Specific Antigen (PSA) level correlations. It is all the more necessary to study prostatic diseases in the present situation as their incidence keeps growing due to extended male longevity past the 60s.3 2 AIMS AND OBJECTIVES 1. To study the incidence of various morphologic types of prostatic lesions encountered at KIMS, Bangalore. 2. To study the age incidence, clinical manifestations and their correlation with benign and malignant lesions. 3. To correlate serum PSA levels with the morphologic types. 4. To study the mimickers of prostatic carcinoma like basal cell hyperplasia, clear cell cribriform hyperplasia and lobular atrophy. 5. To study the incidence of prostatic intraepithelial neoplasia (PIN). 6. To study the histochemical characters of PIN and prostatic adenocarcinoma. 3 REVIEW OF LITERATURE Prostate was believed to be a lobular structure for many years. Prior to 1906, when Home described the middle lobe, prostate was considered to be composed of only 2 lateral lobes. Later the existence of 5 prostatic lobes was proposed – 2 lateral lobes, an anterior lobe, posterior lobe and middle based on embryonic finding.10 PSA was first demonstrated is prostatic tissues, then in seminal plasma, purified from prostatic tissue and finally measured in serum of men. PSA in serum was demonstrated to be a clinically important assay for the monitoring of prostatic carcinoma.10 PSA is secreted into seminal fluid in high concentration, in which it is involved in liquefaction of semen coagulum. PSA was widely used as a clinical marker for prostate cancer by 1988.10 Serum PSA levels increases proportionately with advancing clinical stage.11 Studies have shown that serial increases in serum PSA, increases the incidence of occult carcinoma.12 Several modifications in the estimation of serum PSA are developed. These include a) PSA density: Which is the ratio between the serum PSA value and volume of prostate gland. b) PSA velocity: The rate of change of PSA and the value that distinguishes between men with and without prostatic carcinoma is 0.75ng/ml per year. c) The age specific reference ranges, which is 2.5ng/ml for men of 40-49 yrs of age, 3.5ng/ml for men of 50-59yrs of age, 4.5ng/ml for men of 60-69 yrs years of age and 6.5ng/ml for men of 70-79yrs of age. 4 Studies have revealed that immunoreactive PSA exits in 2 forms, a major fraction bound to α1 anti-chymotrypsin and a minor free fraction. The percentage of free PSA (free PSA/total PSA x100) is lower in prostatic carcinoma than in NH. It helps in discriminating benign and malignant lesion when total PSA level is in “gray zone” of 4 to 10ng/ml. In a study it was found that normal prostate had a higher percent of free PSA (27.7%), than those with NH (20.1%), HGPIN (20.8%) and percentage was lowest in prostatic carcinoma.13 Prostatic acid phosphatase (PAP) a glycoprotein was previously used for detection and monitoring of patients with prostatic carcinoma, however its sensitivity is low and serum PSA has largely supplanted serum PAP.14 Benign prostatic hyperplasia (BPH) was replaced by the term nodular hyperplasia first proposed by Moore in 1943.15 The first study of natural history of NH was reported. Later the first prospective studies on natural history of NH was described. A retrospective review of natural history of NH was studied.16 NH first develops in periurethral zone of prostate.10 NH begins before the age of 30 (early phase), in the mid phase (men between 51-70 yrs) the doubling time is 10yrs and increases to more than 100yrs in men beyond 70yrs.17 Basal cell proliferation in NH was described.18,19 The histological, ultrastructural and immunohistochemical features of florid basal cell hyperplasia was charecterised.20 Clear cell cribriform hyperplasia of prostate was described in 10 patients with clinical diagnosis of NH.21 A rare variant of NH was designated as sclerosing adenosis, analogous to that in breast.22 5 The tissue resected for NH is quite heterogeneous and that the nodules are composed of varying proportion of both epithelial and stromal components.23 The relative proportions of epithelium and stroma are related to development of symptomatic NH with higher percentage of symptomatic NH.24 Inflammation induced release of platelet derived growth factor (PDGF) is a possible, contributing factor in development of BPH.25 Atypical basal cell hyperplasia was described in 12 cases that were confused with adenocarcinoma of prostate.26 The first case of prostatic infarct was reported and it occurs predominantly in large prostates that exhibit NH.27 The clinical spectrum of granulomatous prostatitis was documented and reported in 200 cases.28 A lesion called incidental lymphocytic prostatitis was described.29 A prostate lesion called proliferative inflammatory atrophy was proposed. These are focal atrophic lesions that are associated with chronic inflammation and are adjacent to lesions of PIN, prostatic carcinoma or both.7 Intraductal dysplasia, a potentially premalignant lesion of prostate was described along with reproducible diagnostic criteria.30 It was replaced by term Prostatic Intraepithelial Neoplasia (PIN) introduced in 1989.31,32 It is divided into two grades low grade PIN (LGPIN) and high grade PIN (HGPIN). The prevailing theory, that prostatic carcinoma arose from atrophic epithelium was challenged by postulating that carcinoma arose from active proliferative epithelium.33 Atypical Adenomatous Hyperplasia (AAH) a premalignant lesion of the prostate is the localized proliferation of small glands with in the prostate that can be mistaken for carcinoma.34 A histochemical study of 27 well differentiated prostatic carcinoma cases associated with PIN was done and it was found that lower neutral mucin production by HGPIN was linked to higher proliferative activity of this lesion and also 6 found increased acidic mucin secretion by PIN and prostatic carcinoma.35 The four architectural patterns of high grade PIN was described; tufting, micropapillary, cribriform and flat, the tufting pattern being the most common.36 Rare forms of high grade PIN like signet ring cell, small neuroendocrine and mucinous type were identified.37 These types which are present in invasive carcinoma of prostate provides support for a close relationship between HGPIN and invasive carcinoma. The histology of the central zone located at the base of the prostate adjacent to seminal vesicle was studied and it was concluded to be a potential mimicker of HGPIN.38 Prostatic carcinomas with endometrioid features are best classified and treated as variants of prostatic duct carcinomas.39 A case of adenoid cystic carcinoma with negative immunoperoxidase staining for both PSA as well as prostate specific acid phosphatase was identified and suggested its origin from periurethral glands and metaplastic urethral mucosa.40 For the diagnosis of mucinous adenocarcinoma of the prostate at least 25% of tumor resected during a single procedure should contain lakes of extracellular mucin.41 Mucinous differentiation is common in prostatic adenocarcinoma although it seldom involved more than 25% of volume of any cancer.42 Cribriform adenocarcinoma of prostate has been studied.43 The involvement of prostate by Transitional Cell Carcinoma (TCC) in 43% cases of cystoprostatectomy specimens removed for bladder cancer was reported.44 An unusual variant of prostatic adenocarcinoma called foamy gland carcinoma characterized by abundant xanthomatous cytoplasm was described in needle biopsy specimens.45 7 Prostatic adenocarcinoma with atrophic features was described.46 An unusual pseudohyperplastic pattern of prostatic adenocarcinoma was described.47 Atypical small acinar proliferation (ASAP) suspicious for malignancy in prostatic needle biopsies was evaluated and concluded that the histologic finding of ASAP had a high predictive value for malignancy.48 The significance of intraluminal crystalloids in benign prostate glands on needle biopsy specimen was explored and then concluded that there is no significant higher risk of having cancer on repeat biopsy if crystalloids were not present.49 The Gleason system of histopathologic grading is more reliable, reproducible and an accurate prediction of surgical stage of disease.50,51 The international TNM classification system for prostatic carcinoma has been revised and is most helpful for comparisons between various groups of patients.52 Pathologic factors that influence prognosis of prostatic carcinoma have been studied by many authors. They include grade, extent, cytology, infiltration, inflammatory response and associated changes in prostate.53 The ratio of chips with cancer to the number of chips in Transurethral Resection of Prostate (TURP) specimens was a reliable predictor of stage of the disease.54 The percentage of TUR specimen involved and the tumour volume with progression of prostatic carcinoma was correlated.55 8 Embryonic Development The prostate first appears and starts its development from urogenital sinus during the third month of fetal growth and its development is directed primarily by dihydro testosterone, which is produced from metabolic conversion of fetal testosterone through the action of enzyme 5 α reductase. The prostate forms acini and collecting ducts by arborization that branches into urethra and may be visualized as similar to a small tree with the growth occurring on tips as ducts extend and branch during development. At birth the majority of prostatic acini are lined by squamous epithelium and metaplastic epithelial cells that have scattered secretory activity and lead to cyst formation. Pubertal maturation period starting about age 11 or 12 yrs is characterized by general development of pseudostratified epithelium with basal and secretory cells in all parts of gland and increase in diameter of lumen of ducts. Formation of mature acini starts at about 15-16 yrs. The prostate reaches its mature morphologic and functional structure by age 18-20 yrs and retains this organization for approximately 10 yrs. Around age 30 yrs initial signs of structural and functional disintegration occur and gland gradually loses its functionally organized structure. Thus, prostate is derived from a composite area of urogenital sinus requiring androgen during organogenesis. It undergoes different phases of androgen responsivity perinatally and during puberty.56 9 Anatomy and Histology Prostate gland is an accessory gland of the male reproductive system. Normal adult gland weighs 20 gms ± 6 gms and lies immediately below the base of bladder surrounding the proximal part of urethra. The length of anterior aspect is between 3 and 4 cms and its width between 3.5 and 5cms.Prostate is composed of approximately 70% glandular elements and 30% fibromuscular stroma. Prostate contains a number of individual glands composed of 30 to 50 lobules leading to 15-30 secretory ducts that open into urethra. Prostate is composed of 3 distinct zones (a) peripheral zone comprising of 70% of prostate and common site of PIN and carcinoma, (b) central zone comprising 25% of prostate and surrounds ejaculatory ducts, (c) transition zone comprising 5% of prostate and is the site for NH.57 The glands of prostate are tubuloalveolar and lined with cuboidal or columnar epithelium. Scattered neuroendocrine cells of unknown function is found between the secretory cells. Beneath the epithelial cells flattened basal cells line each acinus and are believed to be stem cells for secretory epithelium. Each acinus is surrounded by a thin layer of stromal smooth muscle and connective tissue. Pathology of Prostate Nodular Hyperplasia NH involves overgrowth of epithelium and fibro muscular stroma of transition zone and periurethral area. It results in varying degrees of urinary obstruction sometimes requiring surgical intervention. 10 Epidemiology NH is extremely common accounting for approximately 4,00,000 partial prostatectomies annually, the most common form of surgery in American men. The age specific prevalence is remarkably similar in populations through out the world. There is rapid increase in prevalence of NH beginning in the 4th decade of life and culminating in 100% prevalence in 9th decade.57 Risk factors Advanced age and an intact androgen supply are the only undisputed risk factors for NH. It does not occur in men castrated before puberty. The risk is lower in patients with androgen resistance or deficiencies. Anti-androgens, gonadotropin releasing hormone agonists and 5 alpha reductase inhibitors also reduce the symptoms. Most epidemiologic studies of environmental and life style risk factors have yielded equivocal results, including studies of medical history, sexual history, smoking, alcohol use, socio economic status and occupational exposure.57 Pathogenesis The development of NH includes 3 pathologic changes: a) Nodule formation b) Diffuse enlargement of transition zone and periurethral tissue c) Enlargement of nodules. 11 In men under 70 yrs of age diffuse enlargement predominates. In men over 70 yrs of age, prostatic enlargement characteristic of NH is dominated by epithelial proliferation and expansile growth of existing nodules probably as a result of androgenic and other hormonal stimulation. The primary mediator of NH is dihydrotestosterone (DTH). This androgen is the major intracellular metabolite of testosterone after its conversion by 5 alpha reductase. Testosterone and DTH bind to nuclear androgen receptors in stromal and epithelial cells in prostate, although, DTH has a 5 fold higher affinity. The nuclear androgen content of NH is greater than that of normal prostatic tissue. Pathology Grossly it is characterized by nodules of varying sizes, which are soft or firm, rubbery and grayish white in colour weighing between 60 to 100 gms. The cut section is nodular. Degenerative changes include calcification and infarction.57 Microscopy The earliest change is stromal proliferation, which contains more smooth muscle and less elastic tissue than normal stroma. The glands are dilated or even cystic often contain an inspissated secretion corpora amylacea. Epithelium is flat or columnar sometimes facing each other in same gland (functional polarization). The epithelium is thrown in to numerous papillary buds and infoldings. The cells have pale cytoplasm and regular central nuclei. Aggregates of lymphocytes are often found within the stroma. In majority of instances, the nodules are adenofibromyomatous nodules, which contain all elements. Small areas of hemorrhagic necrosis surrounded by reactive change in residual 12 epithelium at the margins (squamous metaplasia or transitional metaplasia) may be seen within the nodules.57 Variants of hyperplasia 1) Post-atrophic hyperplasia This is characterized by benign proliferating luminal cells with scanty clear cytoplasm in an atrophic background. The cells often show mild cytologic atypia and luminal mucin may be identified.57 2) Basal cell hyperplasia This is characterized by proliferation of basal cells of more than two cells thick and protrudes into the gland lumen, retaining the overlying secretory luminal epithelium. The hyperplastic basal cells are usually larger than normal, are elongated (spindled) basal cells and is often accompanied by nuclear enlargement.19 3) Cribriform hyperplasia This includes clear cell cribriform hyperplasia and consists of nodule composed of glands arranged in distinctive cribriform pattern. The cells usually have pale-clear cytoplasm and small uniform nuclei with inconspicuous nucleoli.21 4) Sclerosing adenosis It shows striking myoepithelial metaplasia of basal cell compartment as well as a exuberant stroma composed of fibroblasts and loose ground substance.22 13 Associated benign lesions 1) Atrophy This is characterized by small-distorted glands with a flattened epithelium, hyperchromatic nuclei and stromal fibrosis. It is idiopathic and its prevalence increases with advancing age. 2) Atypical basal cell Hyperplasia This is basal cell hyperplasia in which nucleoli are prominent.26 3) Basal cell adenoma This consists of large round circumscribed nodule or nodules that contain uniformly spaced aggregates of hyperplastic basal cells varying from small solid nests to cystically dilated glands. 4) Adenoid basal cell tumour Consists of basaloid cell nests of varying sizes that infiltrate the stroma. The cell nests are often large, round to angular. The peripheral basaloid cells have elongated nuclei and often show palisading. There is prominent cell crowding. 5) Atypical adenomatous hyperplasia (Adenosis) This is localized and circumscribed proliferation of small glands within the prostate that may be mistaken for carcinoma. 14 6) Stromal Hyperplasia with atypia This is characterized by stromal nodules that occur in setting of NH but show increased cellularity and nuclear atypia. These appear as solid stromal nodules or as atypical cells interspersed within benign glands. Stromal nuclei are large hyperchromatic, rarely multinucleated or vacuolated and contain inconspicuous nucleoli. There are no mitoses and necrosis. Stromal hyperplasia with atypia has no malignant potential and the atypical cells are considered to be degenerative. 7) Phyllodes Tumour This is a rare neoplasm of adults. It is a fibroadenoma that shows increased cellularity and cytologic atypia, reminiscent of phyllodes tumour of breast. The glandular epithelium is distorted and lines slit like spaces surrounded by a variably cellular proliferative stroma composed of fibroblasts and smooth muscle cells.58,59 Association of NH and prostate cancer Both show a parallel increase in prevalence with increasing age according to the results of autopsy studies, although cancer lags by 15-20 yrs. Both require androgens for growth and development to occur and both may respond to androgen deprivation treatment. Most cancers arise with in prostate together with NH. Cancer is found incidentally in a significant number (10%) of TURP specimens. NH may be related to prostate cancer arising in transition zone, perhaps in association with certain forms of hyperplasia, but NH is not a pre-malignant lesion or a precursor of cancer. 15 Infarct Infarct of prostate occurs predominantly in large prostates that exhibit nodular hyperplasia. It is found to be present in 18% to 25% cases. The size and number of infarcts are directly related to degree of prostatic hyperplasia. The mechanism of infarct is unknown, but may be related to the presence of prostatic infection or trauma resulting from an indwelling catheter, cystitis or prostatitis all of which may result in thrombosis of intraprostatic portion of urethral arteries.57 Grossly, infarcts vary in size from a few millimeters up to five centimeters. They are speckled grayish yellow and often contain streaks of blood. The peripheral margins are sharp and hemorrhagic. Microscopically, there are sharply outlined areas of ischemic coagulative necrosis involving the glands and stroma. Prominent squamous metaplasia is often seen in the ducts bordering a infarct. This metaplastic change is confined to the expanded ducts, keratinizes only rarely, and does not extend to the surrounding prostatic tissue. Infarct may cause serum elevation of PSA levels, removal of infarcted area return the levels to normal. Prostatic Inflammation The spectrum of prostatitis encompasses a multitude of inflammatory diseases of prostate. These varied forms of prostatitis show marked differences in treatment and clinical outcome and therefore require accurate diagnosis. Patchy acute and chronic inflammatory infiltrate is present in the prostate of most adult men and is considered a 16 normal finding. But when the inflammation is severe and extensive or clinically apparent the term prostatitis is warranted. It is associated with increased serum PSA levels, which return to normal levels following treatment. 1) Acute Bacterial Prostatitis Acute bacterial prostatitis presents with sudden fever, chills and irritative voiding symptoms. The prostate is swollen, tender and warm. The bacteria responsible for acute prostatitis are Escherichia Coli (80%) other enterobacteriacea such as pseudomonas, serratia, klebsiella (10-15%) and enterococci (5-10%).60 Microscopically there are sheets of neutrophils in and around prostatic ducts and acini with desquamated epithelium and cellular debris. Stroma is edematous and hemorrhagic and contains variable numbers of lymphocytes, plasma cells and macrophages. Microabscesses are frequently seen. Diagnosis is based on findings yielded by urine culture and biopsy is contraindicated. Prostatic Abscess A rare complication of acute bacterial prostatitis occurring most commonly in immunocompromised patients. Predisposing factors include urethral instrumentation and use of indwelling catheters. Patients present with prostatic enlargement, fever and retention of urine. A fluctuant mass is occasionally palpated by rectal examination. Prostatic abscess can be caused by aerobic and anaerobic bacteria. 17 2) Chronic Prostatitis The spectrum includes chronic bacterial, chronic abacterial and granulomatous prostatitis. Chronic prostatitis presents with variable clinical features and include frequency, urgency, dysuria, pain in perineum, lower back and testis. Digital rectal examination varies from normal to tender and boggy. Microscopically charecterised by aggregates of lymphocytes, plasma cells and macrophages within the prostatic stroma. The epithelium displays reactive atypia with occasional prominent nucleoli. The histologic findings in combination with clinical manifestations are characteristic of chronic prostatitis. Bacterial and abacterial chronic prostatitis mimic each other clinically and histologically but differentiated by presence or absence of bacteria on culture. Usually gram-positive bacteria are found in cultures of prostatic fluid. a) Chronic bacterial prostatitis Most cases are due to E.Coli infection. In patients with prostatic calculi, and relapsing urinary tract infection (UTI), the stones serve as a nidus of infection, with bacteria embedded in mineral matrix. b) Chronic abacterial (idiopathic) prostatitis This is more common than bacterial prostatitis. Cultures of urine and expressed prostatic secretion yield negative results. This form of prostatitis has a prolonged indolent course characterized by relapses and remissions. 18 3) Granulomatous prostatitis It is a group of morphologically distinct chronic prostatitis caused by a wide variety of inciting agents, including infections, tissue disruption after biopsy and inflammation. It accounts for approximately 1% of benign inflammatory conditions of prostate. Most of the patients have a history of UTI, it is probably caused by blockade of prostatic ducts and stasis of secretions. Epithelium is destroyed and cellular debris, bacterial toxins and prostatic secretions sperm, semen escape into stroma eliciting a intense localized inflammatory response. Grossly the gland is firm to stony hard. Cut section shows obliteration or architecture with formation of yellow granular nodules. Microscopically large nodular aggregate of histiocytes, epitheloid cells, lymphocytes and plasma cells are seen. Caseation necrosis is absent. a) Idiopathic (non-specific) granulomatous prostatitis This makes up the majority of cases. The granulomas are usually non-caseating and associated with parenchymal loss, marked fibrosis and more number of eosinophils in stroma. Xanthoma: It is a rare form of idiopathic granulomatous prostatitis and is characterized by a localized collection of cholesterol laden histiocytes. 19 b) Infectious granulomatous prostatitis Numerous microorganisms can induce granulomatous prostatitis, including bacteria (Mycobacterium tuberculosis, Brucellosis), fungi (Cryptococcus and Blastomycosis), parasite (Schistosoma haematobium) and virus (Herpes zoster). c) Iatrogenic granulomatous prostatitis: This can be caused by surgery, radiation therapy and Bacille Calmette-Guerin (BCG) therapy for bladder cancer.16 i) Post-surgical Granulomatous Prostatitis ii) Post-radiation granulomatous prostatitis iii) BCG-Induced granulomatous prostatitis Malakoplakia This is a granulomatous disease attributable to defective intracellular lysosomal digestion of bacteria. Patients present with obstructive symptoms including frequency, urgency, dysuria, nocturia and fever. This occurs in men over 50 years of age with systemic illnesses and other debilitating conditions. Digital rectal examination reveals diffuse induration suggestive of prostatic carcinoma. E. coli is commonly isolated from urine cultures. Grossly, prostatic malakoplakia is characterized by discrete and confluent soft yellow brown plaques, with central umbilication or ulceration and peripheral hyperemia. Microscopically, the prostatic architecture is effaced by dense sheet-like aggregates of histiocytes (Van-Hansemann cells) admixed with lymphocytes and plasma 20 cells. Intracellular and extracellular Michaelis-Gutmann bodies are seen, appearing as sharply demarcated spherical structures with concentric “owl’s eyes” measuring 5 to 10 μm in diameter. These stain with Periodic acid Schiff (PAS) (polysaccharides), Alizarin red-S (Calcium), Prussian blue (iron), and Von Kossa (anionic component of calcium ions). Gram stain, acid-fast stain and fungal stains are negative. d) Systemic Granulomatous Disease These include i) Allergic (eosinophilic) granulomatous prostatitis ii) Sarcoidosis iii) Rheumatoid Nodules Prostatic inflammation and carcinogenesis: Chronic or recurrent inflammation plays a role in development of carcinoma. Inflammatory cells elaborate numerous microbicidal oxidant that cause cellular or genomic damage to prostate giving rise to cells of PIN and carcinoma.7 Prostatic caliculi Prostatic calculi are seen in about 7% of prostates with nodular hyperplasia. They should be distinguished from those found in the prostatic urethra, which may have their origin in the bladder, ureter or renal pelvis. The corpora amylacea seen in the glands with nodular hyperplasia may act as the nucleus for stone formation as a result of improper drainage, infection of the acini and calcium deposition. Blood clots, epithelial detritus, and bacteria are also present in the stone nucleus. The main inorganic elements are 21 phosphated salts (calcium, magnesium, aminomagnesium, potassium), calcium carbonate and calcium oxalate. Because of their extreme hardness, large prostatic calculi may be mistaken for carcinoma on digital rectal examination. They are radio-opaque and can be easily detected by radiographic examination. PROSTATIC INTRAEPITHELIAL NEOPLASIA (PIN) Definition: It is a neoplastic transformation of lining epithelium of prostatic ducts and acini. The process is confined within the epithelium.61 The term prostatic intraepithelial neoplasia (PIN) was adopted by Bostwick and Brawer (1989) to include all forms of atypical and malignant lesions of epithelial cells confined to the lumens of duct/acinar system as well as similar lesions accompanied by microinvasion. PIN is subdivided into 3 grades: PIN-I, II and III,described by Mc Neal and Bostwick. The Bethesda workshop participants proposed a two-tier system comprising low grade (PIN1) and high grade (PIN 2 and 3).31 PIN consists of dysplasia and proliferation of the normal luminal cell layer lining the prostatic ducts and acini. 22 Diagnostic Criteria McNeal and Bostwick (1986) described the diagnostic criteria for PIN.57 Features Low grade (formerly PIN-I) 1. Architecture Epithelial cell crowding, Similar to low grade PIN; more stratification with irregular crowding and stratification spacing 2. Cytology PIN High grade (formerly PIN 2 and 3) PIN a. Nuclei Enlarged with considerable Enlarged; some size and shape size variation. variation b. Chromatin Normal Increased density and clumping c. Nucleoli Rarely prominent Occasionally to frequently large and prominent similar to invasive carcinoma. 3. Basal cell layer Intact May show some disruption 4. Basement membrane Intact Intact LGPIN The cells within ducts and acini are heaped up, crowded and irregularly spaced and there is variation in nuclear size. Elongated hyperchromatic nuclei and nucleoli are sometime seen but not prominent. Mc Neal summarizes that there is not a sharp line of demarcation between LGPIN and mild degrees of deviation from normal histology.62 HGPIN It exhibits features similar to LGPIN but cell crowding and stratification is more pronounced and there is less variability in nuclear size but prominent nucleoli is diagnostic. It displays four architectural patterns; tufting, micropapillary, cribrifom and flat. 23 Evidence For The Association Of Prostatic Intraepithelial Neoplasia And Prostatic Carcinoma 1. Histology: Similar cyto-architectural features. 2. Location: Both are multifocal with predominant peripheral zone distribution. 3. Correlation with proliferative activity: Both have more than 3 times the proliferative activity of benign glands. 4. Loss of the basal cell layer: PIN-III has loss of basal cell layer similar to invasive carcinoma. 5. Increased frequency of PIN, in the presence of carcinoma: Higher grades of PIN are associated with a statistically significant increase in the frequency of invasive carcinoma. 6. Increased severity of PIN in the presence of carcinoma: Higher grades of PIN are associated with a statistically significant increase in the frequency of invasive carcinoma. 7. Immunophenotype: Both are immunoreactive for cytokeratins 14, 15, 16 and 19 unlike nodular hyperplasia. Both show Ulex Europaeus-1 Lectin binding unlike normal epithelium and nodular hyperplasia. Both show loss of vimentin immunoreactivity 24 unlike nodular hyperplasia. Both show loss of blood group A,B unlike normal epithelium. 8. Age: Incidence and extent of both lesion increase with patient age.63 PIN and Mucin The histochemical study of mucin in PIN by using Alcian blue at pH 1 and pH 2.5 and PASdiastase has demonstrated that there is progressive decrease in neutral mucin from normal prostate to the high grade PIN and progressive increase in acidic mucin in areas of HGPIN and carcinoma. Acidic mucin was absent in LGPIN and normal prostatic tissue.35 SPREAD OF PIN PIN spreads through the prostatic ducts in 3 different patterns: 1) Replacement of the normal luminal secretory epithelium by neoplastic cells, with preservation of the basal cell layer and basement membrane. 2) Pagetoid spread along the ducts, characterized by invasion of neoplastic cells between the basal cell layer and luminal columnar secretory cell layer. 3) Direct invasion through the duct/acinar wall with disruption of the basal cell layer and basement membrane.63 25 CLINICAL IMPORTANCE OF PIN PIN is obviously interesting on a number of grounds. The clinical importance of recognizing PIN is based: 1. On its association with prostate cancer. 2. The association of PIN with serum prostate specific antigen levels: PSA levels in PIN is intermediate between normal and carcinoma. 3. PIN and Ultrasound Transrectal ultrasound (TRUS) of the prostate in cases of PIN presents with hypoechoic areas from which the ultrasound-guided biopsy of that area can be performed and studied for the evidence of PIN and invasive carcinoma 4. PIN and Repeat Biopsy: Biopsy should be repeated at 3 months interval for first two years if the case is PIN and then repeated yearly thereafter. CLINICOPATHOLOGICAL EVALUATION OF PIN On identification HGPIN on biopsy 1) All the tissue should be processed and serially sectioned 2) Basal cell specific anti-keratin monoclonal antibody (34βE12) stains should be done. 3) Transrectal ultrasound with biopsy should be done. 4) Serum prostate specific antigen levels should be monitored. 26 5) Repeat PSA, Trans rectal ultrasound guided biopsy at 3-month intervals for the first two years and repeated yearly thereafter for life should be done.63 Carcinoma of the Prostate Epidemiology Prostatic carcinoma is the sixth most common malignancy in the world (in terms of number of new cases). It represents 9.7% of cancers in men (15.3% in developed countries and 4.3% in developing countries).64 It is the second most common cause of cancer death in men. It is predominantly a disease of elderly men over 50 years. The United States death rate for cancer of prostate is high compared to the other countries. Rates for non-whites are about twice as high as whites in the United States. Prostate cancer is extremely rare in orientals. The urban incidence rate is only slightly higher than rural rate. Aetiology Little is known about the causes of prostatic cancer. The five main factors implicated as aetiologic agents are: a) Age b) Race c) Endocrine system d) Environmental influences. e) Dietary and hereditary factors 27 Age Risk of prostate cancer rises steeply with age. Incidence of clinical disease is low until age fifty and then increases rapidly. The highest incidence is found in the age group 70-79 yrs as compared with 50-60 years of other epithelial cancers.57 Race American blacks have higher incidence than whites. The prevalence rate among Japanese, is in the range of 3-4% and for the Chinese in Hong Kong only one percent as compared with the rate of 50-60 % among the whites in the United States.57 Endocrine System Testosterone diffuses into gland where it is converted by enzyme steroid 5α reductase type II to the more metabolically active form dihydrotestosterone (DHT). DHT and testosterone bind to androgen receptor and receptor ligand complex translocates to the nucleus for DNA binding and transactivation of genes which have androgen responsive elements including those controlling cell division. Environmental Influences Environmental factors as occupational exposure or behavioral factors (sexual life) do not seem to play a clear role. No relationship has been shown between prostatic carcinoma and cigarette smoking, use of alcohol, weight, height, blood group or hair distribution.9 28 Dietary and Hereditary Factors Studies implicate strong association of prostate cancer with intake of animal products especially red meat.7 There is 5-11 fold increased risk among men with two or more affected first degree relatives.4,5 Location Cancers detected on TURP are predominantly within the transition zone. 8% of contemporary TURP specimens disclose carcinoma. When TURP is done without clinical suspicion of carcinoma prostate cancer is incidentally detected in approximately 8-10% of specimens.64 WHO Histological classification of tumors of prostate Epithelial tumors 1) Glandular neoplasms • Adenocarcinoma (acinar) • Atrophic • Psuedohyperplastic • Foamy • Colloid • Signet ring • Oncocytic 29 • Lymphoepithelioma like carcinoma with spindle cell differentiation (carcinosarcoma, sarcomatoid carcinoma) 2) • PIN • PIN Grade III (PIN III) • Ductal adenocarcinoma • Cribriform • Papillary • Solid Urothelial tumors • 3) 4) 5) Urothelial carcinoma Squamous tumors • Adenosquamous carcinoma • Squamous cell carcinoma Basal cell tumors • Basal cell adenoma • Basal cell carcinoma Neuroendocrine tumors • Endocrine differentiation with in adenocarcinoma • Carcinoid tumor • Small cell carcinoma • Paraganglioma • Neuroblastoma 30 6) 7) 8) Prostatic stromal tumors • Stromal tumor of uncertain malignant potential • Stromal sarcoma Mesenchymal tumors • Leiomyosarcoma • Rhabdomyosarcoma • Chondrosarcoma • Angiosarcoma • Malignant fibrous histiocytoma • Malignant peripheral nerve sheath tumor • Haemangioma • Chondroma • Leiomyoma • Granular cell tumor • Hemangiopericytoma • Solitary fibrous tumor Hematolymphoid tumors • Lymphoma • Leukaemia 31 9) 10) Miscellaneous tumors • Cystadenoma • Nephroblastoma (Wilm’s tumor) • Rhabdoid tumor • Germ cell tumors • Yolk sac tumor • Seminoma • Embryonal carcinoma and teratoma • Choriocarcinoma • Clear cell adenocarcinoma • Melanoma Metastatic tumors Acinar Adenocarcinoma Gross Features Carcinomas of prostate arise most commonly in posterior lobe and thus it is readily palpable on rectal examination. The gland may be enlarged or of normal size, nodular and firm in consistency. On transection the neoplastic tissue is gritty, dry, fibrous and homogenously solid and often contains irregular, yellowish areas.1 Histopathological Examination Adenocarcinoma of prostate range from well-differentiated gland forming cancers to poorly differentiated tumors difficult to identify as being of prostatic origin.64 32 Architectural Features Glands are crowded, grow in haphazard fashion, oriented perpendicular to each other and irregularly separated by bundles of smooth muscle indicative of an infiltrative process. Undifferentiated prostatic carcinoma is composed of solid sheets, cords of cells or isolated individual cells.64 Nuclear Features Nuclear enlargement with prominent nucleoli is characteristic.64 Cytoplasmic Features Glands of adenocarcinoma tend to have discrete crisp, sharp luminal border without undulations or ruffling of cytoplasm. In low-grade carcinoma the cells show pale cytoplasm while neoplastic glands have amphophilic cytoplasm.64 Intraluminal Features Prostatic crystalloids, are dense eosinophilic crystal like structures that appear in various geometric shapes rectangle, hexagon, triangle and rod like.64 Extraprostatic Site Extension a) Perineural invasion- malignant gland should completely encircle the nerve. b) Mucinous fibroplasia (collagenous micro nodules)-very delicate loose fibrous tissue, which are ingrowths of fibroblasts sometimes reflecting organization of intraluminal mucin. c) Glomerulations-glands with a cribriform proliferation that is not transluminal. 33 Histologic Variants 1. Atrophic variant Consists of individual small atrophic glands exhibiting marked cytologic atypia 2. Pseudohyperplastic variant Consist of numerous closely packed glands, which are large with branching and papillary infoldings. 3. Foamy gland variant Consists of glands with cells having abundant foamy cytoplasm with a very low nuclear cytoplasmic ratio. Nuclei are small and densely hyperchromatic. 4. Mucinous and signet ring variant Mucinous tumor is accompanied by formation of large amounts of intracellular and extra cellular mucin comprising 25% or more of the tumor. Microglandular, cribriform, comedo, solid and hypernephroid patterns may be present. Signet ring carcinoma grows in solid acinar fashion and is composed of cells with signet ring configuration resulting from intracellular accumulation of mucin. 5. Oncocytic variant Consists of glands with large cells having granular eosinophilic cytoplasm and round to ovoid hyperchromatic nuclei 6. Lymphoepithelioma like variant Undifferentiated carcinoma, consisting of synctial pattern of malignant cells associated with heavy lymphocytic infiltrate. 34 7. Sarcomatoid variant Consisting of features of a carcinoma with sarcomatiod elements showing differentiation towards cartilage bone, and/or skeletal muscle.64 PIN III Incidence of HGPIN is uncommon with a rate of 2.3% to 2.8%.61 Four patterns of HGPIN are noted. 1. Flat-nuclear atypia with out significant architectural changes. 2. Tufting-nuclei become more piled up resulting in undulating mounds of cells. 3. Micropapillary-columns of atypical epithelium that typically lack fibrovascular cores. 4. Cribriform-more complex architectural patterns appear such as roman bridge. Variants of PIN Signet ring variant shows cytoplasmic vacuoles, which displace and indent nuclei. Mucinous variant exhibiting solid intraluminal masses of blue tinged mucin that fill and distend the glands resulting in flat pattern of growth. Foamy variant were foamy PIN glands are large with papillary in-foldings lined by cells with bland nuclei.65 Inverted variant consisting polarization of enlarged secretory cell nuclei toward the glandular lumen with tufted or micropapillary patterns. 35 Small cell neuroendocrine variant consisting of small neoplastic cells with rosette like formations are observed in center of glands. Intraductal variant having overlapping features of cribriform HGPIN and carcinoma. Ductal adenocarcinoma Composed of large glands lined by tall pseudostratified columnar cells with abundant amphophilic cytoplasm. Accounts for 0.2-0.8 % of prostate cancer.66 The patterns are: a. Papillary pattern, which can be seen in both centrally or peripherally, located tumours.67 b. Cribriform pattern, formed by back-to-back large glands with intraglandular bridging resulting in formation of slit like lumens.68 c. Individual gland characterized by single glands. d. Solid pattern, solid nests of tumor cells are separated by incomplete fibrovascular core. 2. Urothelial carcinoma Accounts for 0.7-2.8% of prostatic tumor in adults. It consists of full range of histologic types, papillary urothelial neoplasm, high-grade with in situ components. Urothelial carcinoma fills and expands ducts and often develops central comedo necrosis stromal invasion is associated with a prominent desmoplastic stromal response 36 with tumor cells arranged in small irregular nests cords and single cells. Angiolymphatic invasion is often identified.66 3. Squamous tumors Adenosquamous. Consists of both glandular (acinar) and squamous cell carcinoma components. Occurs after radiation or hormonal therapy of adenocarcinoma.69 Squamous cell carcinoma Tumors with squamous cell differentiation involving prostate, accounts for <0.6% of all prostate tumors. Pure SCC does not contain glandular features and is identical to SCC of other origin. Evidence of keratinization and squamous differentiation are required for diagnosis.70 4. Basal cell tumors Basal cell adenoma. Basal cell carcinoma. Consisting of large basaloid cells with cell nests exhibiting peripheral palisading and necrosis.71 5. Neuroendocrine tumors Neuroendocrine differentiation has three forms: i) Focal neuroendocrine differentiation in conventional prostatic adenocarcinoma – All prostatic carcinoma show focal neuroendocrine differentiation. In 5-10% of 37 carcinomas there are zones with large number of single or clustered neuroendocrine cells detected by chromogranin A immunostaining. ii) Carcinoid tumor (WHO well differentiated neuroendocrine tumor) – Classic features of carcinoid tumour. iii) Small cell neuroendocrine carcinoma (new WHO classification poorly differentiated neuroendocrine) identical to small cell carcinoma of lung.66 Mesenchymal Tumors Sarcomas account for 0.1-0.2% of all malignant tumors. Lesions are classified as prostatic stromal proliferation of uncertain malignant potential (STUMP) and prostatic stromal sarcoma based on degree of stromal cellularity presence of mitotic figures, necrosis and stromal overgrowth.66 Special Staining Methods Various types of mucin and polysaccharides in prostatic cancer can be demonstrated by Alcian Blue, PASd. Normal prostate gland contains mucopolysaccharides or mucoprotiens, which are periodic acid schiff diastase positive. But a shift from neutral to acidic mucin is seen in carcinoma. In occult or early carcinoma, secretion in acinar lumina contains sulfomucin and in some cases shows neutral mucin. In well-differentiated or moderately differentiated carcinomas, there is abundant sialomucin or sulfomucin with a little neutral mucin. In undifferentiated carcinomas, no secretions are generally detected. 38 Grading and staging GLEASON`S MICROSCOPIC GRADING SYSTEM OF PROSTATIC CARCINOMA Stage Description 1 Single, separate, uniform glands in closely packed masses with a definite, usually rounded, edge limiting the area of tumor. 2 Single, separate, slightly less uniform glands, loosely packed (separated by small amounts of stroma), with less sharp edge. 3a Single, separate, much more variable glands, may be closely packed but usually irregularly separated; ragged, poorly defined edge. 3b Like 3a, but very small glands or tiny cell clusters. 3c Sharply and smoothly circumscribed rounded masses of papillary or loose cribriform tumor (papillary intraductal tumor). 4a Raggedly outlined, raggedly infiltrating, fused glandular tumor 4b Like 4a, with large pale cells (hypernephroid). 5a Sharply circumscribed, rounded masses of almost solid cribriform tumor, usually with central necrosis (comedonecrosis). 5b Ragged masses of anaplastic carcinoma with only enough gland formation or vacuoles to identify it as adenocarcinoma.15 Primary grade is assigned to the dominant pattern and secondary to the subdominant pattern. The two numeric grades are added to obtain the combined Gleason grade or score. In tumors with one pattern the number is doubled. Gleason scores are combined into groups : 2 to 4, representing well differentiated cancer, 5 to 6 representing 39 intermediate grade cancer, 7 representing moderate to poorly differentiated cancer and 8 to 10 representing high grade cancer. The major system for staging is TNM system (primary tumor T, regional lymph node N and Metastatic M)73 Primary tumor, clinical (T) TNM Description Primary tumor, clinical (T) Tx Primary tumor cannot be assessed. To No evidence of primary tumor. T1 Clinically inapparent tumor not palpable or visible by imaging. T1a Tumor found incidental, histologic findings in 5% or less of tissue resected. T1b Tumor incidental histologic findings in more than 5% of tissue resected. T1c Tumor identified by needle biopsy (e.g. because of elevated PSA) T2 Palpable tumor confined within the prostate. T2a T2b Tumor involves one lobe. Tumor involves both lobes T3 Tumor extends through prostate capsule T3a Extracapsular extension (Unilateral or bilateral) T3b Tumor invades seminal vesicle (s) T4 Tumor is fixed or invades adjacent structures other than seminal vesicles, bladder neck external sphincter, rectum, levator muscles and or pelvic wall 40 Primary tumor, pathologic (pT) pT2 Organ confined pT2a Unilateral pT2b Bilateral pT3 Extraprostatic extension pT3a Extraprostatic extension pt3b Seminal vesicle invasion pT4 Invasion of bladder, rectum N Regional lymph nodes Nx Regional lymph nodes cannot be assessed. No No regional lymph node metastasis. N1 Metastasis regional lymph node or nodes. M Distant metastatis Mx Distant metastasis cannot be assessed. Mo No distant metastasis. M1 Distant metastasis. M1a Involvement of non-regional lymph nodes. M1b Involvement of bone(s). M1c Involvement of other distant sites. None None. The ten year cancer-specific survival rate for all stages of cancer is about 51% with an estimated cure rate of 32%. 41 Patients with a well differentiated tumors, Gleason total score 2-4 have no clinically progressive cancer, where as one-third of patients with high grade lesions Gleason total score 7-10 will progress into locally invasive or metastatic disease. Patients with less than 5% cancer do not have progressive cancer and do not require therapy, but patients with more than 5% cancer, about one-third of the patients progress to clinically manifest disease. If the tumor is unifocal, the chances of progressive cancer is less, but the patient with more than 2 foci do progress to clinically manifest disease. Even after 40 yrs after the inception of the Gleason grading system it remains one of the most powerful prognostic predictors in prostate cancer.72 Treatment Localized prostatic carcinomas are treated by radical prostatectomy, external radiation therapy and deferred (expectant) treatment. For locally advanced and metastatic tumor hormonal manipulation like estrogens, anti androgens can be used.15 42 Prognosis According to college of American Pathologists (1999) prognostic parameters are divided into: A) Proven to be of prognostic importance and useful in clinical patient management 1. Preoperative serum PSA level 2. TNM stage grouping 3. Histologic grade as Gleason score 4. Surgical margin status B) C) Extensively studied but whose importance remains to be validated : 1) Tumor volume 2) Histologic type 3) DNA ploidy Not sufficiently studied to demonstrate their prognostic value: 1) Perineural invasion 2) Neuroendocrine differentiation 3) Microvessel density 4) Nuclear roundness 5) Chromatin texture 6) Other karyometric factors 7) Proliferation markers 8) PSA derivates 9) Other factors (oncogenes, tumor suppressor genes, apoptosis genes) 43 Immunohistochemical features The two immunocytochemical markers for prostatic epithelium are Prostate specific antigen (PSA) and Prostatic acid phosphatase (PAP). Prostate specific membrane antigen (PSMA) a membrane bound glycoprotein is expressed in all types of prostatic adenocarcinoma. P504 S is an ∝-methyl COA racemase is highly sensitive in adenocarcinoma. Prostatic cancer cells are immunoreactive for androgen and progesterone receptors. Prostatic carcinoma cells are also positive for low molecular weight cytokeratins, Leu 7, Epithelial membrane antigen, carcinoembryonic antigen, B 72.3, cathepsin D, glycoprotein A-80, PTH-related protein and gastric acid proteinase gastricin.15 There are atleast 3 separate useful immunostains for basal cells 34βE12,P63 and CK 5/6 all of which are negative markers for cancer (that is when they stain positively the abnormal acinar focus is negative for cancer).74 Molecular Genetic features Mutation in P53 gene occurs in 40% of prostatic carcinoma. It is a tumor suppressor gene and exerts important regulatory control on apoptosis. Alteration of Rb gene occur in significant number of cases. P27 an inhibitor of cyclin and cyclin dependant kinases show decreased expression and aneuploidy of prostate cancer increases. Expression of several anti-apoptotic members of the bcl–2. gene family (including bcl2, bcl-x and mcl-1) increases during progression of prostatic carcinoma.15 44 METHODOLOGY The present study included prostatic tissue specimen received in the pathology laboratory at Kempegowda Institute of Medical Sciences, Bangalore during the period from December 2003 to December 2005. Brief clinical data were noted from the case records, which included the age presenting symptoms, digital rectal examination findings, serum PSA levels and clinical diagnosis. The specimens thus obtained were fixed in 10% formalin after detailed and careful examination. In case of TURP, approximately 5gm of tissue was processed in one cassette and embedded. The entire tissue was processed in case of TURP and needle biopsy and in cases of prostatectomy representative bits were processed. Then sections 4 to 6 microns thick were prepared. These were stained routinely with hematoxylin and eosin. Other special stains like Alcian blue pH 1, periodic acid schiff (PAS) and Ziehl Neelson were performed wherever necessary. The procedure followed for tissue processing and staining technique are those given in “Cellular Pathology technique” by CFA Culling.75 All the lesions were graded into non-neoplastic and neoplastic lesions. The cases of prostatic adenocarcinoma were graded using Gleason microscopic grading. The clinical and histological data so obtained were analyzed and compared with another similar studies. 45 STATISTICAL METHODS Chi-square and Fisher exact test have been used to test the proportions in association of lesions with Age, Clinical symptoms and Serum PSA values 1. χ 2. Chi-Square Test 2 ∑ (Oi − Ei) = Ei 2 , Where Oi is observed frequency and Ei is Expected frequency Fisher Exact Test Class 1 Class 2 Total Sample 1 a b a+b Sample 2 c d c+d a+c b+d n Total Fisher Exact Test statistic= ∑p= (a + b)!(c + d )!(a + c)!(b + d )! 1 n! ∑ a!b!c!d! Statistical software The statistical software namely SPSS 11.0 and Systat 8.0 were used for the analysis of the data and Microsoft word and Excel have been used to generate graphs, tables etc. 46 RESULTS The present study was carried out from December 2003 to December 2005, at the Kempegowda institute of Medical Sciences, Bangalore. Table 1 Age incidence of the various prostatic lesions Age (yrs) Benign n (%) Malignant n(%) 30 – 39 - - 40 – 49 4(2.94%) - 4(2.67%) P>0.05 50 – 59 19(13.97%) 1(7.14%) 20(13.33%) 0.694 60 – 69 55(40.44%) 6(42.86%) 61(40.67%) 0.861 70 – 79 49(36.03%) 7(50.00%) 56(37.33%) 0.303 80 – 89 9(6.62%) - 9(6.00%) P>0.05 Total 136(100.00%) 14(100.00%) 150(100.00%) - Inference Total n(%) p value - Malignant cases are more commonly seen in the age group of 70-79 years with p=0.303 and benign cases are more common in the age group of 40-59 years with p=0.470. Benign lesions were common in the age group of 60-69 yrs while malignant lesions were common in the age group 70-79yrs. However, when benign and malignant lesions were compared, benign cases commonly occurred in the age of 40-59 yrs and malignant lesions peaked in 70-79 yrs. 47 Graph 1 a Age incidence of the various prostatic lesions Benign Malignant Total 50 45 40 Percentages 35 30 25 20 15 10 5 0 30-39 40-49 50-59 60-69 70-79 80-89 Age in years Graph 1b Incidence of benign and malignant lesions Malignant 9.33% Benign 90.67% 48 Table 2 Clinical presentation of prostatic lesions (Figures in parenthesis are percentages) Benign Malignant Total p value (n=136) (n=14) (n=150) 1.Frequency 50 5 55 0.323 (36.5) (38.5) (36.67) 2.Nocturia 10 2 12 0.278 (7.3) (15.4) (8.00) 3.Urgency 7 7 P>0.05 (5.1) (4.67) 4.Difficulty in voiding 39 3 42 P>0.05 (28.5) (23.1) (28.00) 5.Straining 3 3 P>0.05 (2.2) (20.00) 6.Poor stream 15 15 0.364 (10.9) (10.00) 7.Hesitancy 2 2 P>0.05 (1.5) (1.33) 8.Incomplete voiding 19 5 24 0.036* (13.9) (38.5) (16.00) 9.Hematuria 1 2 3 0.020* (0.7) (15.4) (2.00) 10.Acute retention 31 2 33 0.734 (22.6) (15.4) (22.00) 11.Dysuria 21 5 26 0.050* (15.3) (38.5) (17.33) Inference Incomplete voiding, hematuria, dysuria are significantly associated with the malignant lesions with p≤0.05, poor stream is positively associated with benign lesions with p=0.364. * Significance at 5% Clinical symptoms Frequency was the most common symptom in benign lesions followed by difficulty in voiding, acute retention and dysuria. Malignant lesions had common symptoms of dysuria, incomplete voiding and frequency. However when both lesions were compared poor stream was positively associated with benign lesions and incomplete voiding, hematuria and dysuria were significantly associated with malignant lesions. 49 Graph 2 Clinical presentation of prostatic lesions Benign 40 Malignant 1.Frequency 2.Nocturia 3.Urgency 4.Difficulty in voidi 5.Straining 6.Poor stream 7.Hesitancy 8.Incomplete voidin 9.Hematuria 10.Acute retention 11.Dysuria 35 Percentage 30 25 20 15 10 5 0 1 2 3 4 5 6 50 7 8 9 10 11 Table 3 Digital rectal examination findings Digital rectal examination findings Nodularity 137 Hard nodule 13 Graph 3 Digital rectal examination findings 13 Nodularity Hard nodule 137 Clinically digital rectal examination findings showed nodularity in 137 cases and presence of hard nodule in 13 cases. 51 Table 4 Serum PSA levels in the cases studied PSA values (ng/ml) Benign Malignant 0-4 75 2 4.1-8 25 0 8.1-12 11 3 12.1-15 1 1 16.1-20 5 0 >20.1 19 8 Total 136 14 Inference PSA values with >20.1 are 8.21 times more likely to be malignant with p=0.001 Graph 4 Serum PSA levels in the cases studied 80 70 No.of cases 60 50 40 Malignant 30 Benign 20 10 0 0-4 4.1-8 8.1-12 12.115 15.1- >20.1 20 PSA Value (ng/ml) 52 Table 5 Clinical Diagnoses in the cases studied Clinical diagnosis No. of cases BPH 132(88%) Carcinoma prostate 17(11.3%) Metastatic TCC 1(0.5%) Total 150 Graph 5 Clinical Diagnoses In the cases Studied BPH CA prostate Metastatic ca 17 1 132 Clinically 132 cases were diagnosed as benign prostatic hyperplasia with 17 cases of prostatic carcinoma and one case of metastatic TCC from bladder. 53 Table 6 Nature of prostatic tissue studied Nature of Tissue No. of Cases TURP chips 134 (89.3%) Needle Biopsy 12 ( 8.0%) Total Prostatectomy 4 (2.6%) Total 150 Graph 6 Nature of prostatic tissue studied 140 120 No.of cases 100 80 60 40 20 0 1 2 3 1) TURP 2) Needle Biopsy 3) Prostatectomy 54 Table 7 Microscopic findings in benign lesions studied Microscopic findings No. of cases 1) Adenofibromyomatous hyperplasia 80 2) Basal cell Hyperplasia 5 3) Clear cell Hyperplasia 2 4) Squamous Metaplasia 5 5) Transitional Metaplasia 13 6) Prostatitis 24 7) Abscesses 2 Prostatic intraepithelial neoplasia Lesions No. of cases LGPIN with BPH 12 HGPIN with BPH 1 HGPIN with carcinoma 6 55 Graph 7 Microscopic findings in benign lesions studied 80 70 No.of cases 60 50 40 30 20 10 0 1 2 3 4 5 6 7 Microscopic Findings 1) Adenofibromyomatous 5) Transitional metaplasia 2) Basal cell hyperplasia 6) Prostatitis 3) Clear cell hyperplasia 7) Abscesses 4) Squamous metaplasia 56 Table 8 Microscopic findings in malignant lesions studied Microscopic Findings No. of cases 1) Acinar Pattern 8 2) Trabeculae 4 3) Cords/strands 4 4) Cribriform 6 5) Nests 2 6) Sheets 5 7) Clear Cells 1 8) Perinueral Infiltration 3 9) Metastatic Tumour (TCC High Grade) 1 10) Primary SCC 1 Adjacent Prostatic Tissue a) Adenofibromyomatous hyperplasia b) Inflammation c) HGPIN 10 3 6 The commonest pattern in case of adenocarcinoma was the acinar pattern followed by cribriform and sheets. The adjacent tissue in these cases showed adenofibromyomatous hyperplasia in 10 cases, HGPIN in 6 cases and inflammation in 3 cases. 57 Graph 8 Microscopic findings in malignant lesions studied 8 7 No. of cases 6 5 4 3 2 1 0 1 2 3 4 5 6 7 8 9 10 Microscopic findings 1) Acinar pattern 6) Sheets 2) Trabeculae 7) Clear cells 3) Cords/Strands 8) Perineural Infiltration 4) Cribriform 9) Metastatic TCC 5) Nests 10) Primary SCC 58 Table 9 Incidence of carcinoma with reference to Gleason’s score (maximum Score 10) Gleason`s Score No. of Cases 2 - 3 - 4 - 5 - 6 - 7 7 (58.3%) 8 3 (25%) 9 2 (16.7%) 10 - Total 12 (100%) Graph 9 Incidence of carcinoma with reference to Gleason’s score (maximum Score10) 16.7 0 25 58.3 59 2 3 4 5 6 7 8 9 10 Table 10 Final histopathological diagnosis in the cases studied 1) NH a) Without Prostatitis 98(65.3%) b) With Prostatitis 25(16.6%) 2) PIN a) LGPIN 12(8.0%) b) HGPIN 1 (0.7) 3) Adenocarcinoma 12(8%) 4) Metastatic TCC (from Urinary Bladder) 1(0.7%) 5) Squamous Cell Carcinoma 1(0.7%) Graph 10 Final histopathological diagnosis in the cases studied 90 80 No.of cases 70 60 50 40 30 20 10 0 1 2 3 4 Diagnosis 60 5 61 62 63 64 65 66 67 68 69 70 DISCUSSION Enlargement of prostate is an age related process and incidence increases with increasing age beginning in the forties. The incidence of NH increases from an average of 23 to 88% by the ninth decade.77 Hyperplasias Gross: 1. TURP specimens consisted of multiple gray white soft tissue bits. The weight ranged from 2-30 grams. 2. Needle biopsy consisted of 3-4 linear soft tissue bits measuring 1-1.5 cm in length having thickness of 2-4 μm. 3. Prostatectomy specimens showed hyperplastic lateral lobes. The cut section was nodular with solid and cystic areas (Fig. 1). Most patients over the age of 50yrs will have histological evidence of BPH and many will suffer symptoms from urethral compression. Data indicate that on microscopic examination both epithelium and stroma are involved in varying degrees and predominant pattern is fibroadenomatous hyperplasia. In the present study adenofibromyomatous hyperplasia was seen in 136 cases where hyperplasia of both epithelial and stromal components was seen. According to study by Bostwick et al. NH is composed of varying proportion of epithelium and stroma. The most common nodules reported in their study was adenofibromyomatous nodules which contained all elements.57 (Fig. 2) 71 In the present study 5 cases showed basal cell hyperplasia in the age group of 6575yrs with usual symptom of difficulty in voiding. It is characterized by nodular growth of nests, tubules and cords filled with proliferating small, darkly staining basal cells. Most of the nests of BCH show vertical palisading of basal cells towards the periphery (Fig 3). According to a study, it occurs in the same age group as NH (range 63-80yrs, mean 74yrs) and usual presenting symptom is urinary obstruction.19 In the present study 2 cases showed features of clear cell cribriform hyperplasia (CCCH) in the age group of 55-75yrs. Ayala et al. reported ten cases of CCCH. It is composed of glands with abundant clear cytoplasm and a nodular growth pattern78 (Fig 4). Their ages ranged from 62-87yrs with a mean age of 72yrs.19 The present study showed 13 cases of transitional metaplasia (Fig. 5) and 5 cases squamous metaplasia (Fig. 6) in addition to NH. PROSTATITIS In the present study out of 150 cases, 26 cases had prostatitis. These included Table 11 Showing the incidence of prostatitis Chronic non specific prostatitis 20 (80%) Abscesses 2 (8%) Granulomatous prostatitis 3 (12%) 72 In the study by Granados et al. 25 cases of prostatic abscess were studied which showed sheets of neutrophils in and around the acini79 (Fig. 7). In cases of chronic non-specific prostatitis lymphocytes, plasma cells, macrophages were seen. Bostwick in his study has reported more cases of chronic abacterial as compared to bacterial prostatitis.57 (Fig 8). In the present study 3 cases of non-specific granulomatous prostatitis were noted, out of which one of the case showed well formed epitheloid granulomas with giant cell. There was no evidence of caseation and special stain for AFB was negative (Fig 9). Out of 25 cases of prostatitis urine culture was done in ten cases of which E. Coli was seen in 6 cases, 2 cases showed growth of Klebsiella and one case each of Enterococci and pseudomonas. In a study by Still well 200 cases of granulomatous prostatitis were reported amongst which 138 were of non specific granulomatous prostatitis (69%) and 49 cases were of post biopsy granulomas (3.5%) and 6 cases were of systemic granulomatous disease (3%).28 IHC was done for Leucocyte Common Antigen (LCA) in a case to distinguish distorted lymphocytes in the stroma, (artifactual) which were appearing as signet ring cells. The cells showed positive membrane reaction73 (Fig. 11 and 12). 73 PIN In the present study out of 150 specimens examined 19 cases showed PIN, 12 cases LGPIN which was associated with NH and 7 cases showed HGPIN out of which 1 was associated with NH and the rest 6 were associated with prostatic carcinoma. LGPIN was characterized by epithelial crowding and stratification with anisonucleosis but no prominent nucleoli was observed (Fig. 10). HGPIN was characterized by pronounced epithelial crowding and stratification nuclear enlargement hyperchromasia with prominent nucleoli. None of these lesions showed disruption of basal cell layer and basement membrane (Fig. 13). In our study PIN was seen most commonly in the age group of 70 –79 yrs. In the study by McNeal and Bostwick frequency of PIN was highest in the age group 60 –69yrs.28 In the study by Lee et al, the mean age of PIN was 65 yrs. In our study out of 19 cases of PIN, 13 cases were associated with BPH out of which 12 were LGPIN and 1 was HGPIN. Out of 12 case of adenocarcinoma 6 showed HGPIN in the adjacent prostatic tissue. 74 Table 12 Incidence of PIN in cases of prostates with and without prostatic carcinoma No. of prostates examined PIN without carcinoma (% PIN) PIN with carcinoma (%PIN) McNeal and Bostwick (1986)80 200 43 82 Horinger W (2001) 81 1077 4.7 61.4 Kovi (1988)82 429 46 59.2 Present study 150 9.4 46.1 Studies Brawer concluded in his study that PIN occurred more commonly in prostates with invasive carcinoma than in without. According to his study PIN was found in 73% of prostates with carcinoma and 32% of prostates without carcinoma.31 Table 13 Incidence of HGPIN in prostates with carcinoma Incidence of HGPIN in prostatic adenocarcinoma(%) Authors McNeal and Bostwick (1986)80 Kovi et al (1988) 33 82 Troncoso et al (1989) 33 80 72 83 Quinn et al (1990) W. Horinger (2001)81 100 61.4 Present study 50 The incidence of PIN varies considerably in different studies probably because histological diagnosis of LGPIN shows subjective variation and many studies do not report LGPIN.62 75 The incidence of HGPIN is relatively low in cases of prostatic carcinoma because most of the specimens were TURP which does not have enough material compared to radical prostatectomy which was studied in other studies.33 It has also been suggested that transition zone carcinoma might not be associated with HGPIN.31 Moreover incidence of isolated HGPIN is uncommon in TURP specimens (prevalence 2.3%).84 This is because the site of HGPIN is common in the peripheral zone as compared to transition zone. Prostatic Carcinoma In the present study peak incidence of both PIN and prostatic carcinoma was seen in age group of 70-79 yrs. It has been observed that PIN occur at least a decade earlier compared to prostatic carcinoma. But in present study no such age difference was noted. Many recent studies show a higher incidence of prostatic carcinoma in the age group of 61-70 yrs. However in studies of Moore and Baron the peak incidence was seen in age group of 51-60 yrs. This may indicate change in trend of prostatic carcinoma. 76 Table 14 Age incidence of prostatic carcinoma in different studies Sl. No. Authors 21-30 31-40 41-50 51-60 61-70 71-80 81-90 Total 1 Rich (1934)80 - - - 7 8 12 0 27 2 Moore (1935) 80 - - 9 18 13 7 - 47 3 Baron and Angrist (1941)80 - - 20 26 25 6 - 77 4 Andrews (1949)80 - - 2 7 7 - - 16 5 Edward (1953) 80 - - 3 10 12 3 - 28 6 Franks (1954)80 - - 38 53 70 17 - 178 7 Scott et al (1961) 80 - - - - 36 26 - 62 8 Holund (1980)80 - - 2 7 24 13 - 46 9 Present study - - - 3 6 4 0 13 et al Commonest of prostatic carcinoma is adenocarcinoma. Among 14 cases of prostatic carcinomas encountered 12 (85.1%) are adenocarcinoma (Fig. 14. 15. 16)and one was primary squamous cell carcinoma (Fig. 19) and one case was metastasis from transitional cell carcinoma of bladder (Fig. 20). There is wide variation in the incidence rate of prostate cancer in different parts of the world. The age adjusted incidence rates in Bangalore is 5.2 per 100000.85 The prevalence rate of 9.3% observed in present study is comparable with study by Muruli et al86, which showed a prevalence rate of 8.5%. The increased incidence of prostatic carcinoma in industrialized world is partly due to increased number of cases diagnosed by screening and case finding by serum PSA testing.12 77 Table 15 Prevalence of prostatic carcinoma in different studies Sl. No. Authors No. of prostatic specimens No. of prostatic carcinoma Prevalence 1 Newman et al (1982)80 500 71 14.20 2 Muruli et al (1985)80 222 19 8.56 3 Moore et al (1986)80 143 31 21.68 4 Murphy et al (1986)80 386 66 17.10 5 Yamabe et al (1986) (Japan)80 191 24 12.57 6 Yamabe et (Netherlands)80 452 57 12.61 7 Eble and Tejada (1986) 80 700 132 18.56 8 Rohr (1987)80 457 65 14.22 9 Present study 150 12 9.3 al (1996) Histochemical stains for mucin in the present study demonstrated neutral mucin in all LGPIN cases and in these cases acidic mucin was absent. All cases of carcinoma showed positivity for acidic mucin.35 (Fig.18) Present study showed 90% BPH/Prostatitis, 0.7% HGPIN and 9.3% prostatic carcinoma. W. Horinger et al in his study reported 73.1% BPH/Prostatitis, 4.7% HGPIN and 22.2% prostatic carcinoma.81 Present study showed 90.7% of BPH and 9.3% prostatic carcinoma. Anna Pacelli and David G. Bostwick reported incidence of 81.7% of BPH and18.3% adenocarcinoma87. 78 In the present study all cases of adenocarcinoma were showing basement membrane disruption, with 3 cases showing perineural invasion (Fig. 17). These pathological changes have been well documented in literature,88 Carter HB and Partin AW reported the distribution of Gleason score in 703 men with clinically localized prostatic carcinoma.89 Table 16 Comparative incidence of carcinoma with reference to Gleason’s score Studies Gleason score No.of patients(%) Carter HB and Partin AW 2-4 64(9) 5 168(24) 6 303(43) 7 130(19) 8-10 38(5) Total 703(100) 2 0 3 0 4 0 5 0 6 1(8.3) 7 6(50) 8 3(25) 9 2(6.7) Total 12(100) Present study 79 In the present study low grade adenocarcinoma was not detected probably because these lesions are usually asymptomatic Bob Djavan et al reported 22% incidence of carcinoma. Mean age of patients was 67 yrs and mean Gleasons score was 6.90 Table 17 Incidence of prostatic carcinoma mean age and mean Gleason’s score Studies Incidence of prostate cancer Mean age Mean score Bob Djavan 22% 67 6 Present study 9.3% 73 7 Richard Babajan et al studied 151 men between September 1998 and September 1999 at university of Texas M.D Anderson cancer center. Cancer was detected in 24.5% of men biopsied. Mean age of all 151 men was 62yrs (range 43-74 ). Gleason score was 6 in 12 patients 7 in 1 and 8 in 1.91 Table 18 Incidence of prostatic carcinoma, median age and Gleason’s score Studies Richard J Babian et al Present study Incidence of prostatic carcinoma Median age 24.5% 62 8% 70 80 Gleason score 6-12 patients 7-1 patient 8-1 patient 7-7 patients 8-3 patients 9-2 patients Serum PSA Levels NH is a common cause of serum PSA elevation and accounts for 60-70% of cases .Studies of patients with histologically confirmed NH have shown that 21-86% have elevated serum PSA levels. The degree of elevation is modest (4.1-10ng/ml).92 In the present study 36 cases had modest elevation of PSA levels and remaining 25 cases the degree of elevation was severe with 19 cases having levels more than 20.1 ng/ml. This is because these cases of NH was associated with prostatitis, abscesses and granulomatous prostatitis. According to a study in chronic prostatitis serum PSA was high in 99% of the cases. Also prostatic manipulations including cystoscopy, needle biopsy and TURP are known to elevate serum PSA levels. In a small percentage of men digital rectal examination (9%), prostatic massage (6%) and trans rectal ultrasound scanning (11%) result in elevation of serum PSA levels.93Acute urinary retention also elevates the PSA values.94 Serum levels of PSA were frequently elevated in patients with PIN ranging from0.3 to 22.3 mg/ml (mean 4.0).95 In the present study, they showed normal levels (< 4 ng/ml) In the present study, 12 case of prostatic carcinoma had high levels of PSA (>12ng/ml), however 2 cases had PSA levels below 4ng/ml this can be attributed to study in which it was reported that prostate cancers detected at lower PSA levels are more likely to have a small volume (less than 0.5ml) and are of low grade.96 81 LIMITATIONS OF STUDY 1. In the cases of needle biopsy the tissue obtained was from the transition zone and not from the peripheral zone, hence diagnosis of malignancy may be missed inspite of strong clinical suspicion of malignancy. 2. Immunohistochemistry was done in one case. Due to lack of facilities and financial constraints it was not done in all the suspected cases. SCOPE FOR IMPROVEMENT The correct diagnosis of mimickers of adenocarcinoma should be made in order to prevent radical prostatectomy, which has high rate of morbidity in elderly males. Since serum PSA was increased in few benign and most of the malignant cases, newer modalities of measuring PSA like PSA density, PSA velocity, age specific reference rates should be adopted to distinguish between benign and malignant lesions. Diagnosis of HGPIN should be included since it is a precursor of adenocarcinoma. Most of the cases of prostatic adenocarcinoma encountered were of high grade. Since low-grade lesions are usually asymptomatic, awareness of serum PSA level estimation, digital rectal examination should be brought among elderly males who are prone for malignancy. Male individuals with a positive family history for prostatic carcinoma must undergo relevant screening test. 82 CONCLUSION 1. Out of 150 prostatic specimens studied benign lesions were common, which accounted for 90.7% and malignant lesions accounted for 9.3%. 2. Benign lesions were common in the age group of 60-69yrs, with common symptoms of frequency, difficulty in voiding and acute retention. 3. Prostatitis was commonly encountered in the age group of 60-69yrs. 4. Among 136 cases of benign lesions, 111 cases (81.7%) were diagnosed as nodular hyperplasia without prostatitis and 25 cases (18.3%) were diagnosed as nodular hyperplasia with prostatitis. 5. Histologically varied patterns of hyperplasia were noted in 25 cases of 98 nodular hyperplasia without prostatitis. Basal cell hyperplasia in 5 cases (5.1%), clear cell cribriform hyperplasia in 2 cases (2%), transitional metaplasia in 13 cases (13.2%) and squamous metaplasia in 5 cases (5.1%). 6. PIN accounted for 8.6% (13 cases) with a peak incidence in age group of 70-79 years. LGPIN accounted for 8% (12 cases) and HGPIN accounted for 0.6% (1 case). 83 LGPIN were reported in view of complete description of histologic variants. Incidence of isolated HGPIN was low because most of the specimens studied included TURP, which is from transition zone, and HGPIN is common in peripheral zone. However HGPIN was noted in adjacent prostatic tissue in 6 cases (50%) of prostatic adenocarcinoma out of 12 cases of adenocarcinoma. 7. Malignant lesions showed a peak incidence in the age group of 60-69 years with a common symptoms of frequency, incomplete voiding and dysuria. 8. In benign lesions serum PSA was normal in 75 cases (55.1%). Modest elevation [4.1-10 ng/ml] was seen in 36 cases (26.4%) and marked elevation [>10 ng/ml] in 25 cases (18.3%) due to associated conditions like chronic prostatitis, granulomatous prostatitis and abscesses. In malignant prostatic lesion 8 cases ( 57.1%) showed marked elevation in serum PSA levels (>20 ng/ml). 9. Among the malignant lesions, commonest lesion was prostatic adenocarcinoma (85.8%). 10. A rare neoplasm of the primary squamous cell carcinoma of prostate was encountered. 11. Gleason’s microscopic grading was adopted for grading prostatic adenocarcinoma in which the commonest Gleason score was score 7 (58.3%), followed by score 8 (25%) and score 9 (16.7%). Low-grade lesions were not encountered probably because these lesions are usually asymptomatic 84 12. Acidic mucin was demonstrated in the lumens, in all cases of prostatic adeno carcinoma demonstrated by Alcian Blue pH1. 13. In this study we did not encounter any case of infarct, calculi, malakoplakia, lowgrade adenocarcinoma and other rare variants of malignant neoplasms. 85 SUMMARY 1) Total of 150 prostatic specimens were included in this study, received in the Department of Pathology at Kempegowda Institute of Medical Sciences, Bangalore from December 2003 - December 2005. 2) Most of the specimens received were TURP 134 (89.3%), followed by needle biopsy 12 (8%) and total prostatectomy 4 (2.6%). 3) The cases were studied in relation to age, common presenting symptoms and was correlated with serum PSA levels. 4) Benign lesions were seen after the age of 50 years. Benign lesions included Nodular hyperplasia without prostatitis (81.7%) and nodular hyperplasia with prostatitis (18.3%). 5) 13 cases (8.6%) of cases showed PIN of these 8% (12 cases) were LGPIN, which was reported in view of complete description of histologic variants. 1 case (0.6%) of HGPIN, was noted. 6) Out of 98 cases (70.6%) of NH, histologically varied patterns of hyperplasia were noted in 25 cases with basal cell hyperplasia in 5 cases (5.1%), CCH in 2 cases (2.0%), Transitional metaplasia in 13 cases (13.2%) and squamous metaplasia in 5 cases (5.1%). 7) Out of 25 cases of prostatitis (16.6%), 20 cases (80%) had chronic non-specific prostatitis and 2 cases of prostatic abscesses (8%) and 3 cases (12%) of granulomatous prostatitis. 86 8) Clinical diagnosis of prostatic carcinoma was done in 17 cases, out of which 8 cases correlated histologically and one case sent as metastatic transitional cell carcinoma was confirmed to be the same. 9) In benign cases serum PSA was normal in 75 cases (55.1%). Modest elevation [4.1-10 ng/ml] was seen in 36 cases (26.4%) and marked elevation [>10 ng/ml] seen in 25 cases (18.3%). 10) Digital rectal examination finding showed nodularity in 137 cases and hard nodule in 13 cases. 11) Both PIN and malignancy showed peak in the age group of 60-79 years 12) In the malignant lesion, prostatic adenocarcinoma was the commonest (85.8%), followed by one case of primary squamous cell carcinoma (7.1%) and one case of metastatic transitional cell carcinoma (7.1%). 13) The commonest pattern encountered in prostatic adenocarcinoma is acinar pattern followed by cribriform, sheets, trabeculae, cords, strands and clear cells. 14) Gleason’s microscopic grading was adopted to grade prostatic adenocarcinoma cases and score 7 (58.3%) was the commonest. 15) All 12 cases of prostatic adenocarcinoma showed basement membrane disruption with 3 cases showing perineural invasion and 3 cases showing inflammation. 16) All cases of adenocarcinoma showed acidic mucin in the lumens as demonstrated by Alcian blue pH1 and all the cases of LGPIN showed negativity for Alcian Blue and positive PASd. 87 BIBLIOGRAPHY 1. 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Prevalence of prostate cancer among men with prostate specific Antigen level ≤4.0 ng per milliliter. NEJM 2004; 350 (22): 2239-2246. 101 PROFORMA Name: Laboratory Number: Age: IP/OP Number: Chief complaints: Past History: Personal History: Family History: General Physical Examination: Systemic Examination: Digital Rectal Examination: Clinical Diagnosis: Investigations: Preoperative total PSA : Type of surgical procedure done: Histopathological Examination: Gross: Microscopy: Final Diagnosis: 102 PROCEDURE FOR SPECIAL STAINS Periodic Acid-Schiff with Diastase Predigestion 1) Bring sections to water. 2) Treat with diastase (saliva) for 30 minutes. 3) Washed in running tap water for 10 minutes. 4) Oxidize for 5 minutes in 1% aqueous periodic acid. 5) Wash in running water for 5 minutes and rinse in distilled water. 6) Treat with Schiff reagent for 15 minutes. 7) Wash for 10 minutes in running water. 8) Counterstain with haematoxylin. 9) Dehydrate, clear and mount in synthetic resin. RESULTS PAS positive substances Bright red Nuclei Blue Other tissue constituents Yellow 103 ALCIAN BLUE (pH1) 1) Bring sections to water. 2) Stain in 1% Alcian blue 8 GX in 0.1 N hydrochloric acid for 30 minutes. Rinse briefly in 0.1 N HCL. 3) Blot dry with fine filter paper to prevent the staining which sometimes occurs after dilution with water (which will change the pH) in washing. 4) Dehydrate in alcohol, clear in xylene and mount in resinous mountant. Results Acid sulphated mucosubstances stain blue. Ziehl-Neelson staining method for AFB (Tubercle Bacilli) 1) Bring the sections to water 2) Treat the sections with strong carbol fuchsin 3) Heat the underside of the slide till it fumes 4) The sections are subsequently treated with coconut oil to prevent shrinkage for 10 minutes. 5) Decolorize with 20% sulfuric acid solution 6) Counter stain with Loeffler`s methylene blue solution for 2-5 minutes) 7) Rinse in water, dehydrate clear and mount in a resinous mountant. Results AFB Red Nuclei Blue Other tissue constituents Pale blue 104 KEY TO MASTER CHART Presenting complaints Diff voiding → difficulty in voiding Incmp voiding → incomplete voiding Clinical investigations USG → Ultrasound findings DRE → Digital rectal examination PSA → Prostate specific antigen TURP → Transurethral resection prostate G. Hyp → Glandular hyperplasia FMH → Fibromuscular hyperplasia TR MT → Transitional Metaplasia CC METP → Clear cell cribriform hyperplasia SQ METP → Squamous metaplasia MONO → Mononuclear cells BCH → Basal cell hyperplasia PIN → Prostatic intraepithelial neoplasia C/S → Urine culture and sensitivity CA → Carcinoma GL SCR → Gleason score Microscopy 110 Final → Final histopathological diagnosis NH → Nodular hyperplasia LGPIN → Low-grade prostatic intraepithelial neoplasia HGPIN → High-grade prostatic intraepithelial neoplasia SCC → Squamous cell carcinoma TCC → Transitional cell carcinoma 111 1 Anadigowda 75 1466/03 2 ThammeGowda 55 1504/03 3 KempeGowda 70 1535/03 4 Chikkachandraiah 55 1569/03 3M 5 Shivappa 70 1651/03 1M 6 Mariyappa 75 1875/03 7 Huchaiah 67 1984/03 8 Hombalaiah 65 1987/03 USG DRE dysuria acute retention Clinical investigation haemturia incmp voiding hesitancy strain diff voiding Lab No. frequency Age (Yrs) urgency Name No. nocturia Sl. poor stream Presenting complaints 1D 15D G.Hyp FMH TR CC MT METP + + hard nodule 38 ?Ca TURP, 10cc + + 1D 3M nodularity 2.1 BPH TURP, 5cc + + 2D nodularity 3 BPH TURP, 4cc + + + + 2D 2D 2Y nodularity 2.3 BPH TURP, 5cc + + nodularity 2.72 BPH TURP 10cc + + nodularity 32 ?Ca TURP, 8cc + + nodularity 230 ?Ca TURP, 10cc + + 9 Kencharangaiah 75 2126/03 1M nodularity 1.46 BPH TURP, 5cc + + 10 Mudalappa 77 2127/03 1M nodularity 4.2 BPH TURP, 10cc + + 11 Muddegowda 65 2219/03 nodularity 4.32 BPH TURP, 10cc + + 12 Thimme gowda 65 2220/03 nodularity 7.6 BPH TURP, 5cc + + 13 Channakeshava 53 42/04 nodularity 3.2 BPH TURP, 5cc + + 14 Honappa 65 65/04 2D nodularity 5.8 BPH TURP, 10cc + + 15 Nanjundegowda 69 69/04 1D nodularity 3.23 BPH TURP, 7cc + + 16 Doddachari 54 223/04 nodularity 16.45 BPH TURP, 10cc + + 17 Kempaiah 73 225/04 1D nodularity 3.2 BPH TURP 5cc + + 18 Doddavenkatappa 62 289.04 2D nodularity 3.2 BPH TURP, 5cc + + 19 Singaraiah 70 400/04 nodularity 0.49 BPH TURP, 6cc + + 20 Narayanappa 71 455/04 21 Kenchegowda 75 460/04 22 Basavaiah 76 479/04 4D 23 Shenoy 62 526/04 2M 24 Srinivas 57 527/04 1M 25 Munireddy 45 55/04 1.5 Y 26 Nanjappa 82 559/04 3M nodularity 2.1 27 Krishoji rao 85 569/04 15D nodularity 9.86 28 Krishoji rao 85 601/04 2D 15D Prostate nodularity enlarged 9.86 BPH 1M 2D 7D 20D 1M 1Wk 4D 1D 1D GL SCR GROSS TURP,10cc 1M 3.2 Microscopy BPH 5M nodularity PSA (ng/ml) clinical diagnosis MASTER CHART PIN Others CA nests,comedo,cords,cribr 9 iform, perinural invasion + prostatitis + cribriform,trabeculae, 8 sheets,inflam, HGPIN small acinar,sheets,trabeculae, 7 inflam, HGPIN + + + Prostatitis, C/S- E. coli + Perineural invasion small acinar,sheets,cords,trabe 8 culae nodularity 5.24 BPH TURP, 10cc + + nodularity 2.1 BPH TURP, 30cc + + nodularity 8.26 BPH TURP, 7cc + + + nodularity 3.2 BPH TURP, 5cc + + + nodularity 0.21 BPH TURP, 4cc + + nodularity 2.1 BPH TURP, 3cc + + BPH TURP, 5cc + + BPH Needlebiopsy + + TURP, 6cc + + 109 SQ METP MONO BCH + + + + prostatitis, C/SPseudomonas MASTER CHART Final NH Adenocarcinoma High-grade NH with prostatitis NH NH NH Adenocarcinoma High-grade Adenocarcinoma High-grade NH NH NH NH NH NH NH NH with prostatitis NH NH Adenocarcinoma High-grade NH NH NH NH NH NH NH NH with prostatitis NH 109 29 Pandu rangaiah 60 619/04 30 Muniyappa 40 630/04 31 Siddalingaiah 70 730/04 1M 32 Neelakantaiah 60 764/04 1M 33 Doddaappaiah 85 778/04 34 Aravind 65 793/04 35 Rangappa 60 868/04 36 Metayappa 60 893/04 37 Kadrinaganna 82 996/04 3M 38 Krishnaswamy 65 1019/04 5M 39 Narasaiah 65 1021/04 1M 40 Nanjaiah 70 1045/04 41 Dharmaiah 70 1103/04 42 Basavaraju 58 1111/04 43 Ramanna 70 1159/04 44 Narasimhamurthy 70 1242/04 45 Cheluvarangaiah 65 USG 1M 1D 3M 1D 2D 1M 2M 8D 1D 2M 3D 2M 1243/04 2M 5.46 BPH TURP, 2cc + + BPH TURP, 4 cc + + nodularity 5.2 BPH TURP, 6cc + + 280 ?Ca Needle biopsy nodularity 1 BPH TURP, 40 cc + + nodularity 15.22 BPH TURP, 20 cc + + nodularity 12 BPH TURP, 3 cc + + plus nodularity 1.13 BPH TURP, 10 cc + + nodularity 3.8 BPH TURP, 5 cc + + nodularity 3 BPH TURP, 5 cc + + nodularity 1.2 BPH TURP, 5 cc + + nodularity 4.17 BPH TURP, 20 cc + + nodularity 0.9 BPH TURP, 8 cc + + nodularity 2.4 BPH TURP, 8 cc + + 7.26 BPH TURP, 20 cc + + 3 BPH TURP, 20 cc + + 4.2 BPH TURP, 10 cc + + + nodularity 1M Prostate nodularity enlarged 6D 6D G.Hyp FMH 2.1 1Y 46 Kalappa 70 1308/04 47 Vishakantegowda 75 1309/04 48 Vishakantegowda 75 1404/04 49 Janaki Ram 76 1437/04 50 Madaiah 61 1495/04 2M 1M 51 Chota sab 60 1586/04 2M 2M 52 Marilinge Gowda 75 1617/04 hard nodule 21 ?Ca Needle biopsy + + 53 Balakrishna 50 1654/04 1D nodularity 6 BPH TURP, 5 cc + + 54 ThammeGowda 80 1665/04 3D Prostate nodularity enlarged 16 BPH + + 55 Siddiahashetru 60 1812/04 1 Yr nodularity 32 BPH Needle biopsy + + 56 Krishnappa 72 1895/04 5M nodularity 0.3 BPH TURP, 20 cc + + 57 Siddiahashetru 60 1905/04 1 Yr nodularity 32 BPH TURP, 10 cc + + 58 Nanjaiah 70 2006/04 6M nodularity 25.12 BPH TURP, 12 cc + + 59 Narasappa 60 2007/04 1Y nodularity 1.65 BPH TURP, 5cc + + 5Y hard nodule 20.9 ?Ca Needle biopsy 15D 15 D hard nodule 24.8 ?Ca Needle biopsy + 15D 15 D hard nodule 24.8 ?Ca + + nodularity BPH TURP, 10 cc + + 5 Yr 2M 1D 1 Yr 1Y 1M 6M 5.2 GL SCR GROSS nodularity Prostate nodularity enlarged 1D PSA (ng/ml) Microscopy nodularity Prostate enlarged 5D 3M DRE dysuria acute retention Clinical investigation haemturia incmp voiding hesitancy strain diff voiding Lab No. frequency Age (Yrs) urgency Name No. nocturia Sl. poor stream Presenting complaints clinical diagnosis MASTER CHART nodularity 1.2 BPH TURP, 10 cc + + 1.2 BPH TURP, 10 cc + + 109 SQ METP MONO BCH PIN + Others CA prostatitis LG PIN plus small acinar,cords,sheets 7 LG PIN + + + prostatitis, C/Senterococcus + + Prostate nodularity enlarged TURP, 25 cc TR CC MT METP C/S-Klebsiella + prostatitis LG PIN small acinar, cords, sheets, 7 MASTER CHART Final NH NH with prostatitis NH with LGPIN Adenocarcinoma High-grade NH, LGPIN NH NH NH NH NH NH NH NH NH with prostatitis NH NH NH Adenocarcinoma: High grade NH NH with prostatitis NH NH NH NH NH NH with LGPIN NH NH NH NH NH 109 USG DRE dysuria acute retention Clinical investigation haemturia incmp voiding hesitancy strain diff voiding Lab No. frequency Age (Yrs) urgency Name No. nocturia Sl. poor stream Presenting complaints PSA (ng/ml) clinical diagnosis MASTER CHART Microscopy G.Hyp FMH 60 Subania 69 2075/04 15D 15D nodularity 1.14 BPH TURP, 5 cc + + 61 Hanumaiah 54 2079/04 3M 15D nodularity 3.67 BPH TURP, 10 cc + + Prostate nodularity enlarged 9.47 BPH TURP, 20cc + + 6.32 BPH TURP, 10 cc + + 0.8 BPH TURP, 10 cc + + 4 BPH TURP, 5 cc + + 3 BPH TURP, 8 cc + + nodularity 2.6 BPH TURP, 20 cc + + nodularity 0.4 BPH TURP, 10 cc + + nodularity 0.6 BPH TURP, 6 cc + + nodularity 5 BPH TURP, 4 cc + + 3M 62 Shivanna gowda 69 2115/04 63 Siddegowda 52 2131/04 64 Veerabhadraiah 50 2142/04 65 Nanjunde gowda 45 2275/04 2M 66 Muniyappa 48 2513/04 3M 67 Rangaswamy 70 2525/04 2M 68 Singari gowda 52 2562/04 69 Shivanne gowda 50 2703/04 70 Needasiddya 55 2704/04 71 Abdulbasheer 68 2754/04 1M nodularity 21.72 BPH TURP, 8 cc + + 72 Md. Hussain 72 2769/04 20D nodularity 2.8 BPH TURP, 20 cc + + 73 Linge Gowda 60 2815/04 1M nodularity 4.8 BPH TURP, 15 cc + + 74 Somaiah 78 2832/04 nodularity 3 BPH TURP, 1 cc + + 75 Manchaiah 71 2866/04 5D nodularity 2.5 BPH TURP, 15 cc + + 76 Huchegowda 65 2886/04 3M 3M nodularity 4 BPH TURP, 2 cc + + 77 Nanjappa 72 2887/04 6M 1M hard nodule 12 ?Ca TURP, 5 cc + + 78 Kenchaiah 63 2892/04 4M nodularity 3.2 BPH TURP, 2 cc + + 79 Somasiddaiah 65 2893/04 nodularity 2.2 BPH TURP, 5 cc + + 80 Puttaiah 53 2894/04 nodularity 11 BPH TURP, 5 cc + + 81 Appaji 71 2950/04 82 Krishna 61 2986/04 83 Byrappa 75 2989/04 84 Chikkajalappa 65 3014/04 85 Doddamuthappa 70 3017/04 86 Paramashivaiah 66 3113/04 87 Basavaraj 70 3116/04 88 Lingappa 70 19/05 1M 3M 1M nodularity Prostate nodularity enlarged nodularity Prostate nodularity enlarged 2Y 1M 1M 1M 1M 4M 6M 2M 10D 1D 2M 6D 2M 1M 1D 1M 25D nodularity 1 BPH TURP, 5 cc + + 1Y nodularity 6.35 BPH TURP, 10 cc + + 2M nodularity 8.4 BPH TURP, 5 cc + + nodularity 0.8 BPH Prostatectomy + + nodularity 3.2 BPH TURP, 8 cc + + nodularity 29.3 ?Ca Needle biopsy + + hard nodule 13.3 ?Ca TURP, 4 cc + + nodularity BPH TURP, 6 cc + + 1M 2M 1M 1Y 6M 6.6 GL SCR GROSS 109 TR CC MT METP SQ METP MONO BCH PIN Others CA LG PIN granulomatous AFB-ve LGPIN + Prostatitis, C/S- Klebsiella LG PIN granulomatous, AFB - Ve LG PIN LG PIN + Prostatitis, C/S-Klebsiella small acinar,cribriform, 7 HGPIN MASTER CHART Final NH NH with LGPIN NH with granulomatous prostatitis NH NH NH NH with LGPIN NH NH NH NH NH with prostatitis NH NH NH NH with LGPIN NH SCC NH NH NH with granulomatous prostatitis NH NH NH with LGPIN NH NH with LGPIN NH with prostatitis Adenocarcinma HighGrade NH 109 89 Venkatappa 60 34/05 90 Poornashivaiah 61 38/05 3M 1M 1D USG DRE dysuria acute retention Clinical investigation haemturia incmp voiding hesitancy strain diff voiding Lab No. frequency Age (Yrs) urgency Name No. nocturia Sl. poor stream Presenting complaints PSA (ng/ml) clinical diagnosis MASTER CHART Microscopy GL SCR GROSS G.Hyp FMH nodularity 2.4 BPH TURP, 10 cc + + nodularity 29.3 BPH TURP, 20 cc + + 109 TR CC MT METP SQ METP MONO BCH PIN Others CA MASTER CHART Final NH NH 109 MASTER CHART Honnappa 65 65/05 92 Muddachinnaiah 65 86/05 93 Gopalakrishnashetty 85 99/05 94 Channappa 65 140/05 95 Narayanappa 70 152/05 96 Mustafa 62 176/05 97 Ranganathaiah 60 187/05 98 Kenchappa 70 303/05 99 Lakshmagowda 78 326/05 100 Raghavan 69 332/05 101 Jayaramanna 65 357/05 1Y 104 Thammaiah 70 447/05 6M 105 Mukanna 80 480/05 106 Doddachantappa 64 483/05 107 Lakshmegowda 70 498/05 108 Hanumanthaiah 65 536/05 109 Holegowda 78 616/05 110 Marigowda 62 662/05 3M 111 Laxmaiah 60 938/05 1Y 112 Thammegowda 65 1082/05 113 Kariyanna 60 1198/05 114 Madigappa 75 1222/05 115 Mohammad 52 1467/05 118 Venkatappa 60 1549/05 119 Arvind 60 1572/05 120 Venkateshappa 55 1600/05 + + TURP, 10 cc + + nodularity 2.2 BPH TURP, 10 cc + + nodularity 2.86 BPH TURP, 10 cc + + nodularity 1.8 BPH TURP, 10 cc + + ?Ca Needle biopsy dysuria acute retention haemturia TURP, 10 cc BPH 3.2 BPH TURP, 15 cc + + BPH TURP, 20 cc + + 6M nodularity 20.2 BPH Needle biopsy + + nodularity 4 BPH TURP, 15 cc + + nodularity 2.8 BPH TURP, 20 cc + + 6M 1D 6M 1Wk 15D 1D 10D 3M 1D 1M 2M 1D 2D 6M 5D 20D GL SCR SQ METP MONO BCH PIN Others + Prostatitis + prostatitis, C/S-E-coli + Prostatis CA + nodularity 8.83 BPH TURP, 20 cc + + nodularity 2 BPH TURP, 10 cc + + nodularity 16.8 BPH Prostatectomy + + nodularity 1.07 BPH TURP, 10 cc + + nodularity 3.08 BPH Prostatectomy + + nodularity 2.27 BPH TURP, 25 cc + + hard nodule 12 ?Ca TURP, 15 cc + + TCC bladder hard nodule 87.98 ?Ca Needle biopsy + + small acinar,trabeculae,cribrifo 8 rm 4 BPH TURP, 10 cc + + 8 BPH TURP, 20 cc + + hard nodule 96.48 ?Ca TURP, 30 cc nodularity 2.09 BPH TURP, 20 cc + + nodularity 22.71 BPH TURP, 30 cc + + nodularity 8 BPH TURP, 30 cc + + Prostate nodularity enlarged nodularity 20M TR CC MT METP + 2.7 1Y 1M BPH 4.75 nodularity 15D 6M 0.6 nodularity nodularity 3M 3M 1M nodularity DRE G.Hyp FMH 3M 3M 2M Microscopy GROSS PSA (ng/ml) hard nodule 38.98 1Y 6M 378/05 1520/05 incmp voiding 1D 380/05 1531/05 hesitancy 15D 60 78 strain 1Y 70 72 1M 1D 103 Ningaiah 117 Chinappa USG 1D 3M 102 Puttaswamaiah 116 Dodda kamaiah Clinical investigation clinical diagnosis 91 diff voiding Lab No. frequency Age (Yrs) urgency Name No. nocturia Sl. poor stream Presenting complaints + + cribriform,cords,clear cells + + Prostatitis + Prostatitis + nodularity 3.6 BPH TURP, 10 cc + + LGPIN nodularity 10 BPH TURP, 30cc + + LGPIN nodularity 4 BPH TURP, 8cc + + hard nodule 12 ?Ca TURP, 20cc + + nodularity BPH TURP,4 cc + + 6.2 109 7 trabeculae,cribriform,infl 7 am + prostatitis, C/S- E. Coli LGPIN MASTER CHART Final NH NH NH with prostatitis NH NH Fibro myo hyperplasia NH with prostatitis NH NH NH NH NH NH with prostatitis NH NH NH NH TCC bladder Adenocarcinoma high Grade NH NH Adenocarcinoma high Grade NH with prostatitis NH with prostatitis NH NH with LGPIN NH with LGPIN Adenocarcinoma High Grade NH with prostatitis NH with LGPIN 109 MASTER CHART 72 1716/05 122 Siddappa 70 1840/05 123 Ramappa 56 1868/05 124 Thimmarayappa 55 1870/05 125 Chikkanna 60 1923/05 126 Chunche gowda 70 1941/05 127 Puttaswamy 70 1968/05 128 Kempanna 70 2051/05 129 Kalamaregowda 75 2107/05 130 Narayanaswamy 55 2121/05 131 Ramaiah 60 2251/05 132 Rehaman 65 2228/05 133 Inayathulla 60 2304/05 134 Venkatappa 76 2339/05 USG 1M 2D 1Y 1D 1W 20D 1M 4M 2M 20D 1D 2M 1M 2M 80 2352/05 60 2408/05 137 Jayanna 50 2438/05 138 Sabja baig 60 2510/05 139 Channegowda 65 2528/05 140 Hanumanthaiah 70 2592/05 141 Hombegowda 65 2593/05 142 Vasudevareddy 70 2602/05 2M 143 Thimmappa 72 2603/05 3M 144 Abdulwahab 60 2769/05 145 Naidu 75 2770/05 146 Rangaiah 60 2879/05 12.3 BPH TURP, 6 cc + + nodularity 47.25 BPH TURP, 10 cc + + nodularity 4 BPH TURP, 5cc + + nodularity 5.75 BPH TURP, 5cc + + nodularity 1.7 BPH TURP, 20 cc + + nodularity 15.25 BPH TURP, 25 cc + + nodularity 0.6 BPH TURP, 20cc + + nodularity 25.32 BPH TURP, 15 cc + + nodularity 1 BPH TURP, 1 cc + + prostatitis, C/S-E-coli + + Prostatitis + prostatitis + TURP, 6 cc + + 2M nodularity 12 ?Ca Needle biopsy + + nodularity 2.6 BPH TURP, 12 cc + + 6M nodularity 3.2 BPH TURP, 4cc + + nodularity 38.35 BPH TURP, 15 cc + + nodularity 4 BPH TURP, 15 cc + + 15D nodularity 2.6 BPH TURP, 15 cc + + 15D nodularity 3 BPH TURP, 4 cc + + nodularity 3.8 BPH TURP, 3 cc + + nodularity 0.8 BPH TURP, 15 cc + + nodularity 8 BPH TURP, 20 cc + + nodularity 27.6 BPH TURP, 8 cc + + Granulomatous,HGPIN nodularity 21.61 BPH TURP, 15 cc + + prostatitis nodularity 1 BPH TURP, 12 cc + + 1M 2D 3M 147 Chikkaboraiah 75 2899/05 2M 148 Ningegowda 75 2924/05 1M 149 Chikkappa 62 2977/05 1M + Prostatitis + + Abscess nodularity 18.02 BPH Prostatectomy + + + nodularity 6.97 BPH TURP, 2 cc + + + nodularity 1.89 BPH TURP, 2 cc + + 109 7 perineural invasion small acinar,nests 9 + BPH 15D small acinar,inflamm HGPIN abscess + 3 1M CA LGPIN nodularity 2D 3M nodularity Others 1M 2M 2M + PIN + 1D 15D 1M + + GL SCR SQ METP MONO BCH Needle biopsy 2M 1M + TURP, 3 cc TR CC MT METP ?Ca 4M 6M TURP, 30 cc BPH G.Hyp FMH hard nodule 51 1M 136 Ramu BPH 68.3 PSA (ng/ml) 1M 3M 135 Lingappa 10 nodularity DRE Prostate nodularity enlarged 2D Microscopy GROSS dysuria acute retention haemturia incmp voiding hesitancy strain Clinical investigation clinical diagnosis 121 Chikapa diff voiding Lab No. urgency Age (Yrs) nocturia Name No. frequency Sl. poor stream Presenting complaints MASTER CHART Final NH with LGPIN Adenocarcinoma High Grade NH with prostatic abscess NH with prostatitis NH NH NH NH with prostatitis NH NH NH NH with prostatitis NH with prostatitis Adenocarcinoma high Grade NH NH NH with prostatits NH NH NH NH NH NH with abscess NH with granulomatous prostatitis NH with prostatitis NH NH NH NH 109 MASTER CHART 62 2980/05 2M 1M USG DRE dysuria acute retention haemturia incmp voiding hesitancy strain diff voiding urgency Lab No. Clinical investigation nodularity PSA (ng/ml) 30 Microscopy clinical diagnosis 150 Muthegowda Age (Yrs) nocturia Name No. frequency Sl. poor stream Presenting complaints GROSS BPH TURP, 25 cc G.Hyp FMH 109 + + TR CC MT METP GL SCR SQ METP MONO BCH + PIN Others prostatitis, C/S-E. coli CA MASTER CHART Final NH with prostatitis 109
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