CHARLES HOLBROOK MULLER
Curriculum Vitae
Department of Urology 356510
University of Washington School of Medicine
Seattle, Washington 98195-6510
(206) 543-9504 [email protected]
Aug 2013
PERSONAL
Born October 5, 1947, New York, N.Y
EDUCATION
B.A. in Zoology, University of Colorado, Boulder, June 1969
M.A. in Biology, University of Colorado, Boulder, June 1972
Ph.D. in Zoology, University of California, Berkeley, December 1976
POST-GRADUATE EDUCATION
Senior Fellow; Research Associate, Department of Biological Structure, University of Washington School of
Medicine, Seattle, WA, Sept. 1980- Dec. 1982
Research Fellow, Department of Zoology, University of California, Berkeley, CA, April - September, 1979
NIH Postdoctoral Fellow, Department of Zoology, The University of Texas at Austin, May 1977 - April 1979
Postdoctoral Research Zoologist, Dept. of Zoology, University of California, Berkeley, Dec. 1976 - May 1977
FACULTY POSITIONS
Lecturer in Urology, Dept. of Urology, University of Washington School of Medicine, July 1995 – present
Adjunct Lecturer, Dept. of Biological Structure, University of Washington School of Medicine, Oct 2006 –
present
CLINICAL POSITIONS
Director, Male Fertility Laboratory, Department of Urology, June 1993 – present
PREVIOUS POSITIONS
Interim Director, University Reproductive Care (IVF) Laboratory, Aug 2004 –2010
Departments of Urology and Environmental Health, University of Washington Schools of Medicine and Public
Health, Seattle, Research Scientist 4, June 1993- June 1995
Department of Obstetrics and Gynecology, University of Washington School of Medicine, Research Scientist 3,
July 1991- March 1992; Research Scientist 4, April 1992-May 1993
Director, Reproductive Biology - Andrology Laboratory, Department of Obstetrics and Gynecology, 1983-1993
Research Assistant Professor, Dept. of Obstetrics and Gynecology, and Joint Research Assistant Professor,
Dept. Biological Structure, University of Washington School of Medicine, January 1983-June 1991
Visiting Assistant Professor, Dept. Biol. Sciences, Bowling Green State Univ., Ohio, Sept. 1979-June 1980
Charles Holbrook Muller
Curriculum Vitae p. 2
HONORS
Charlie Garcia Distinguished Service Award for 2008-9, Office of Multicultural Affairs, University of
Washington School of Medicine
Elected to Executive Council, American Society of Andrology, 2008-2011
Co-Author of Award Presentation, American Society for Reproductive Medicine, Oct 2005
Section Editor, Journal of Andrology, 2003-2004
Award Poster, American Fertility Society, 1992
Nominated for 'Instructor of the Year' by the Class of 1987, sponsored by the University of Washington TYEE
Recipient of University of Washington Graduate School Research Fund Awards, 1985 and 1988-1989
Recipient of University of Washington Biomedical Research Support Grant, 1983-1984
NIH R23 New Investigator Research Award, Principal Investigator, July 1, 1982-June 30, 1985
NIH F32 Postdoctoral Fellowship Award, 1977 to 1979, University of Texas at Austin
NIH Predoctoral Trainee Sept. 1972 to Dec. 1976, Department of Zoology, University of California, Berkeley
Phi Sigma Outstanding Undergraduate in Biology, University of Colorado, 1969
CERTIFICATION
High Complexity Clinical Laboratory Director (American Board of Bioanalysis), 1995-present
Clinical Consultant (American Board of Bioanalysis), 2012-present
Male Fertility Lab Certification: CLIA 50D0701043, State of Washington MTS-2730
PROFESSIONAL ORGANIZATIONS
American Society of Andrology, Member since 1983; Andrology Laboratory Workshop Committee (2002 –
2004; 2008-present; Chair 2013-present), Student Affairs Committee 1989-92; Industrial Relations
Committee 1993-2002 (Chair 1999-2002); Laboratory Science Forum (2005-present); Local Arrangements
Committee Chair 2004-2006; Future Meetings Committee 2006-present; Andrology Certification
Committee 2006-2010; Executive Council Member 2008 - 2011.
American Society for Reproductive Medicine (formerly American Fertility Society), Member since 1988;
Andrology Laboratory Certification Committee 1988-90; Member, Reproductive Biology Special Interest
Group (RBSIG); RBSIG Research Development Committee, 1992-1995.
Society for Male Reproduction and Urology, Charter Member since 1995; Member, Nominating Committee,
1996, Program Committee, 1997.
Society for the Study of Reproduction, Member since 1981; Education Committee 1984-85; Co-chair,
Reproductive Cell Biology Committee 1986-87; Chair, Local Arrangements Committee, 1986-88 for
annual national meeting at the University of Washington, August 1-4, 1988; Program Committee 1992-93.
American Society of Zoologists, Member 1970-1990; Co-Organizer, Western Regional Conference on
Comparative Endocrinology, University of Washington, Seattle, March 26-27, 1982.
BOARD OF DIRECTORS or MEDICAL ADVISORY BOARD
Pacific Reproductive Services, San Francisco and Pasadena, CA. 2009-present.
Seattle Sperm Bank / European Sperm Bank, USA, Seattle, WA. 2008-present.
Rhododendron Species Foundation, Federal Way, WA. Board 2003-present. President, 2009-2013.
EDITOR and EDITORIAL BOARD
Journal of Andrology Editorial Board 1998 – 2000; Section Editor 2003-2004
Rhododendron Species Editorial Board 2006-present
Charles Holbrook Muller
Curriculum Vitae p. 3
CURRENT IRB-APPROVED PROTOCOLS
"Markers of Sperm Cryopreservation Success" IRB #42890 (PI) June 20, 2012- June 19, 2014. In collaboration with
Dr. Thomas Walsh and resident physicians, we investigate predictors of success of sperm freezing. We will
identify sperm measures (including motility, kinematics, total numbers, morphology), semen analysis
measures, processing variations, and clinical diagnoses that may predict the recovery of motile sperm postthaw and the usefullness of thawed sperm for reproductive purposes.
"Physiology of Ejaculated Human Sperm" IRB # 06-0264 (J. Amory, PI; C. Muller Co-Investigator), March 2009 present. Human male subjects are recruited by Dr. Amory or by Dr. Muller through the Male Fertility Lab
(for infertile men), and screened for health, reproductive hormones, and semen analysis. Subsequently,
semen samples from consented donors are used by co-investigators. In Muller's lab, sperm function tests
(e.g., acrosome reaction, hyperactivation), effects of stimulators on purified sperm, effects of contraceptive
agents, and DNA fragmentation are studied. Semen and sperm samples are processed for analysis of
retinoic acid by collaborator Dr. Nina Isoherranen. In collaboration with Dr. Kim Woodrow in
Bioengineering, sperm are used in development of novel contraceptive methods. In collaboration with Dr.
Jeffrey Riffell in Biology, sperm chemotaxis is studied using laminar flow devices and automated sperm
kinematic analysis in Muller's lab. In collaboration with Dr. Margaret Shnorhavorian of Seattle Childrens
Hospital, sperm from survivors of childhood cancer are analyzed. In collaboration with Dr. Michael
Skinner of Washington State University, samples are collected and processed by Muller's lab for analysis of
the human sperm epigenome.
"Intrauterine Insemination for Couples with HIV Discordancy" IRB # 37365 (B. Houmard, PI) June 2009-2013. In
this study, we investigate the most efficient laboratory method for removing HIV virus particles or cells
expressing HIV from spiked semen samples. Second, with further IRB approval, we use this approach in a
clinical setting for HIV discordant couples seeking a pregnancy where the man is HIV-positive. The Male
Fertility Lab processes the sperm samples for this study.
“Human Testis Analysis” IRB # 38286, (Muller, PI) April 2010- present. Fixed and sectioned normal (noncancerous) human testes from men with various diagnoses are analyzed for spermatogenesis by
morphological and molecular markers of Sertoli cell and germ cell function. Comparisons are made, where
possible, with semen analysis and reproductive and medical histories. Samples are obtained from Pathology
files, and analyzed by Drs. Muller, Walsh and Amory.
"Retinoic Acid and Spermatogenesis in Man" IRB # 37399 (Amory, PI; Muller Co-Investigator). 2010-present.
Retinoic acid and its metabolites are measured in semen, sperm, and testicular tissue obtained by biopsy
from men with various fertility diagnoses. The Male Fertility Lab / Andrology Research Lab processes the
sperm samples for this study.
"DNA Fragmentation, Oxidative Stress and Pro-Inflammatory Cytokines in CP/CPPS" IRB # 27656 (Muller, PI).
2006-1/5/2014 (continuation for data analysis). Chronic pelvic pain in men, formerly known as “prostatitis”
is a common complaint. We thought that some of this pain might be due to oxidative stress and subsequent
damage to sensory nerves. We hypothesized that such oxidative stress would be manifested by oxidative
damage to the men’s sperm cells, which accumulate and do not repair DNA damage. We measured sperm
DNA damage, reactive oxygen species and anti-oxidants in semen of pain and control subjects. We found
that, on average, the controls had higher levels of DNA damage and reactive oxygen activity than the pain
subjects. However, pain subjects had lower levels of the anti-oxidant catalase in semen. Pain subjects also
had more white blood cells, higher nitric oxide levels, and higher levels of the chemokine MCP-1. Thus,
our original basic hypothesis is not supported, but several details are worth further investigation. Research
protocols have been completed by the Andrology Research Lab. We are still analyzing data.
Charles Holbrook Muller
Curriculum Vitae p. 4
RECENT IRB-APPROVED PROTOCOLS
"Predictive markers of sperm cryopreservation success in men with cancer" IRB # 36608 (A. Jaime; C Muller, PI
Faculty Sponsor and Co-Investigator), June 2009-Dec 2011. The objective of this research project is to
investigate the sperm dysfunctions of men diagnosed with various types of cancer who are undergoing
radiation and/or chemotherapy, before, during and after cryopreservation at the Male Fertility Lab. Semen
analysis data and data from cryopreservation cycles pre- and post-thaw are used to investigate the
relationships among types of cancer and sperm function and cryosurvival.
PRESENT RESEARCH FUNDING
Co-Investigator, Project 1: "Novel ALDH1a2 Inhibitors for Male Contraception” (John Amory, PI)
Contraceptive Centers Program, Program Project “Basic and Clinical Research in Male Contraception” (W.
Bremner, Program Director) NIH NICHD U54. Submitted Nov 2011; Funded Oct 2012. 7/1/12 – 6/30/17.
10% support.
Mentor, “Multidisciplinary Training in Benign Urology” (H. Wessells, PI) NIH T32 Training Grant, Submitted
May 2011. Funded.
Co-Investigator, “BDADS for Male Contraception”, NIH NICHD U01HD060488 (J Amory, PI), funded
$1,073,777, 2/15/09-1/31/14. 8% support.
Principal Investigator, “Effects of GI198745 , on Semen Characteristics”, Clinical Trial funded by Glaxo
Wellcome Inc., Aug 1998 - July 2000; ongoing continuation 2000 – present.
RECENT SUBMITTED RESEARCH FUNDING
Co-Investigator, Project 1: Retinoic Acid and Spermatogenesis in Man” (John Amory, PI) Specialized
Cooperative Centers Program in Reproduction and Infertility Research, Program Project “Clinical and
Basic Studies in Male Reproduction” (W Bremner, Program Director) NIH U54. Submitted July 2013.
Project 1 funding $1.5M requested. 10% support.
Co-Investigator, Retinoic Acid and Spermatogenesis in Man” (John Amory, PI) NIH R01. Submitted Feb 2012.
Not Funded.
Co-Investigator, Project 1: Retinoic Acid and Spermatogenesis in Man” (John Amory, PI) Specialized
Cooperative Centers Program in Reproduction and Infertility Research, Program Project “Clinical and
Basic Studies in Male Reproduction” (J. Amory, Program Director) NIH U54. Submitted May 2011.
Project 1 funding $1.5M requested. 25% support. Not funded.
Principal Investigator, Subcontract, “Epigenomic Biomarkers for Adult Onset Disease” (M. Skinner,
Washington State Univ, PI), NIH R01. Subcontract funding $375,000 requested 7/11-6/16. Submitted Sept
2010. Not funded.
Co-Investigator, Project 1: Retinoic Acid and Spermatogenesis in Man” (John Amory, PI) Specialized
Cooperative Centers Program in Reproduction and Infertility Research, Program Project “Clinical and
Basic Studies in Male Reproduction” (W. Bremner, Program Director) NIH U54. Submitted June 2010.
Project 1 funding $1,565,107 requested 4/11 – 3/16. Not funded.
Principal Investigator, Subcontract, “Sperm and Blood Epigenome Biomarkers for Human Adult Onset
Disease” (M. Skinner, Washington State Univ, PI), Supplement for NIH R01, submitted 2/10. Subcontract
funding $224,909 requested 7/10-6/13. Not funded.
Principal Investigator, Subcontract “Identification and Analysis of Functional Roles of Sperm Cell
Glycoproteins” (Platt, University of Oxford, PI), Medical Research Council UK, submitted 5/2009.
Subcontract funding $80,224 requested 2/1/2010 – 1/31/2013. Not funded.
Co-Investigator, “Targeting Cofactors to Reduce Male HIV -1 Infectivity” in response to “Transmission of
Human Immunodeficiency Virus (HIV) in Semen” (S Graham, PI), NIH, submitted 6/2009. $2.9M
requested. Not funded.
Charles Holbrook Muller
Curriculum Vitae p. 5
Co-Investigator, “FSHβ-Derived Peptide-Gonadotoxin Conjugates for Pet Sterilization” (J Amory, PI),
Michelson/Found Animals Foundation, submitted 5/2009. Funding $776,837 requested 7/1/2009 –
6/30/2012. Not funded.
PAST RESEARCH FUNDING
NIH GRANTS
Co-Investigator, "Retinoic Acid and Spermatogenesis in Man", NIH NICHD U54 HD042454 (W. Bremner, PI;
T.Walsh, Pilot Project PI), funded $30,075, 3/01/2011-2/29/2012
Principal Investigator, “DNA Fragmentation, Oxidative Stress and Pro-Inflammatory Cytokines in CP/CPPS”,
NIH NIDDK (CPCRN Ancillary Project on 5 U01 DK065202 with C. Yang, formerly R.E. Berger, PI),
funded 3/1/05 – 2/28/08. No-cost extension to 2/28/09.
Collaborator, “Cytogenetic analysis of recombination in the human male” NIH HD 042720 (T. Hassold, WSU,
PI), 04/01/04 – 3/2009.
Co-Investigator, “Interstitial Cystitis Collaborative Research Network (ICCRN)”, NIH NIDDK (C. Yang, PI) 5
U01 DK065202, funded 2003-2008.
Collaborator, “Evaluation of Heterogeneity in Urothelial Cells in Interstitial Cyatitis and Clinical Management
of the Disease by Recombinant Modulators” NIH NIDDK (ICCRN Ancillary Project with C. Yang, J.
Bassuk) 2004-2008.
Co-Investigator, Chronic Prostatitis Collaborative Research Network (CPCRN), NIH NIDDK (R. Berger, PI)
funded 2003-2006.
Co-Investigator, “Glycosphingolipid Inhibition and Spermatogenesis in Man” NIH local pilot grant (J. Amory,
PI) 2004-2006.
Collaborator, “Urological Studies of Idiopathic Lower Tract Syndromes”, NIH DK-38955 (J. Krieger, PI), 1991
-2001
Collaborator, “Regulatory Sub-Units of Protein Kinase A: Involvement in male germ cell formation and
function”, NIH P50 HD-12629 (S. McKnight, P.I.), 1996 - 2001
Collaborator, “Protein Kinase A Knockout Mice”, NIH FIRST Grant (R. Idzerda, P.I.), 1996 - 2001
Consultant, “Enzyme Studies of PSA” (T. Takayama, P.I.) NIH 1996-2001
Consultant, “HIV Shedding in Semen: Causes and GU Tract Consequences”, NIH DK-94-009 (J. Krieger, PI),
1994 - 1998.
Co-Investigator, “Human Sperm Structure and Function Analyses as Biomarkers of Effects in Metal Exposed
Human Populations” (Project # 9 of “Effects-Related Biomarkers of Toxic Exposures” {Superfund
Program Project}, David Eaton, Program Director), NIH--National Institutes of Environmental Health,1992
- 1995.
Co-Investigator, “Semen as a Biomarker of Effects Among Lead Exposed Men”, National Institutes of
Occupational Health and Safety, (Harvey Checkoway, P.I.), 1992- 1994.
Co-Investigator, “Regulation and Function of SPARC in Male Reproduction”, NIH Research Grant R01 HD25059 (Robert Vernon, Principal Investigator), funded August 1, 1989- July 31, 1992.
Principal Investigator, "Regulation of Sperm Surface Molecules", NIH Population Research Center Grant P50
HD-12629 ("Studies in Reproductive Medicine," William J. Bremner, Director), Sept. 1985 - March 1991.
Principal Investigator of Subcontract, "Animal Models of Male Infertility," NIH Research Grant RO1 HD15594 (Curtis E. Chubb, Principal Investigator), July 1985-June 1987.
Principal Investigator, "Hormonal Control of Sperm Maturation" and "Glycolipid Surface Antigen of
Spermatogenic Cells," NIH Population Research Center Grant P50 HD-12629 (C. Alvin Paulsen, Director),
September 1, 1982-August 31, 1985.
Principal Investigator, "Hormonal Regulation of the Epididymis and Sperm Surface," NIH Research Grant, R23
HD-16211 (New Investigator Research Award), July 1, 1982-June 30, 1985.
Postdoctoral Fellow, "Male Reproduction: Steroid Hormones and Spermatogenesis," National Institutes of
Health National Research Service Award, F32 HD-05503, funded April 15, 1977 to April 14, 1979.
Charles Holbrook Muller
Curriculum Vitae p. 6
OTHER GOVERNMENT GRANTS and CONTRACTS
Co-Investigator, "Coho Salmon Pituitary Hormones," NSF Research Grant, PCM 83-15405, (W.W. Dickhoff,
Principal Investigator), funded January 1985-January 1987.
PRIVATE or INDUSTRY GRANTS, GIFTS and CONTRACTS
Principal Investigator, “A Multicenter, Randomized, Double-Blind, Placebo-Controlled Trial to Evaluate
Spermatogenesis in Healthy Male Subjects during Administration of BMS-562086-CN148-014-030”,
Clinical Trial funded by Bristol-Myers Squibb, BMS-562086, 7/1/07 – 6/30/09.
Co-Principal Investigator, “Prostatitis Research”, Paul G. Allen Foundation for Medical Research (R. Berger,
PI), funded 1993-2006.
Laboratory Director, “A randomized controlled trial of two vasectomy techniques” funded by EngenderHealth
(formerly AVSC International) and Family Health International, 1999-2002
Co-Investigator, “Occupational Lead Exposure and Male Reproductive Health: The Use of Semen as a
Biomarker of Effect”, Industrial Hygiene Research Award -- Environmental Health, (Harvey Checkoway,
P.I.), January 1992-June 1992.
UNIVERSITY-SPONSORED RESEARCH GRANTS
Co-Investigator, “Reproductive Toxicity Assessment of Lead Exposed Men”, BSRG -- Environmental Health,
(Elaine Faustman, P.I.), January 1992-December 1992.
Co-Investigator, “Semen Quality as a Biomarker of Effect Among Lead Exposed Men”, BSRG –
Epidemiology, (Harvey Checkoway, P.I.), October 1 1991-March 31 1992.
Principal Investigator, "Sperm Fertilizing Ability Mediated by Lipid Transfer Proteins", University of
Washington Graduate School Research Fund, funded January 1988 - June 1989.
Principal Investigator, "Regulation of Male Fertility," Biomedical Research Support Grant, funded October,
1983-October, 1984.
Charles Holbrook Muller
Curriculum Vitae p. 7
TEACHING EXPERIENCE
CURRENT TEACHING - University of Washington
Chair, Microscopic Anatomy, Summer Pre-Matriculation Course (HuBio 510S) (Lectures 50%, Laboratories
100%) Summer 2005 - present.
CME Courses: at least one lecture given at each course
Faculty Member, 2009, Andrology Laboratory Workshop, Leukocytes and Spermatocytes: Seminal Clues to
Reproductive Health, American Society of Andrology, Philadelphia PA.
Faculty Member, 2005, College of Reproductive Biology, Quality Sperm for Quality Embryos: Sperm Do
Matter, American Association of Bioanalysts, Tampa, FL.
Faculty Member, 1999, Andrology Workshop, Current Sperm Function Testing, American Society of
Andrology, Louisville, KY.
Faculty member, 1993, Post-Graduate Courses Technological Advances in the Diagnosis and Management of
Male Infertility and Controversies in Reproductive Biology and Technology, 49th Annual Meeting,
American Fertility Society, Montreal.
Wyeth Visiting Professor, Jan. 1993, New York, NY.
Faculty member, 1992, Post-Graduate Course In Vitro Fertilization, 17th Annual Meeting, American Society of
Andrology, Bethesda, Maryland.
Faculty member, 1989, Serono Symposium on Fertilization in Mammals, Boston, Mass.
PAST TEACHING
University of Washington
Chair, Microscopic Anatomy, School of Medicine (HuBio 510), School of Dentistry (BioStr 541) (Lectures
50%, Laboratories 25-33%) Fall 2006 - 2010.
Microscopic Anatomy, Medical School (HuBio 510) and Dental School (BioStr 541) (Lectures 10-60%,
Laboratories 33-100%) 2004-2005
Microscopic Anatomy, Summer Pre-Mat Course (HuBio 510S) (Lectures 50%, Laboratories 100%) 2002-2004
Microscopic Anatomy (BioStr 541) Special course (Lectures 100%) 2003
Microscopic Anatomy (HuBio 510, BioStr 512, BioStr 541) (Lectures 10%; Laboratories 25%) 1980-1985
General Anatomy (BioStr 301) (Lectures 10%).1982
Gross Anatomy (BioStr 440, HuBio 553) (Lectures 10%; Laboratories 20%). 1980-1982, 2011
Reproduction (HuBio 545, 565) (Lectures 10%).1980-1985
Reproduction and Development Seminar (BioStr 593) (Graduate seminar 100%).
Honors Seminar (A&S 350H) (Undergraduate seminar 100%).
Basic Science Review Course, Dept. of Urology (for residents) (Lectures 10%) 1995, 1997.
Bowling Green State University (Full responsibility for developing and teaching courses) 1979 – 1980.
Human Anatomy and Physiology (Lectures 100%; Laboratories 33%; for pre-professional students).
Human Biology (Lectures 100%; for non-science majors).
Biology of Reproduction (Graduate seminar 100%).
University of Colorado (Teaching Assistant or Teaching Associate) 1972 – 1976.
General Biology (Laboratories 20%).
Comparative Vertebrate Anatomy and Embryology (Lectures 10%; Laboratories 50%).
Experimental Embryology (Lectures 10%; Laboratories 100%).
Histology (Lectures 10%; Laboratories 100%).
Vertebrate Embryology (Lectures 10%; Laboratories 50%).
Guest Lecturer (5-10% responsibility) at Berkeley, Texas and Washington
Comparative Endocrinology, University of California, Berkeley and University of Washington
Human Physiology, University of Texas at Austin
Reproductive Endocrinology (Zool 469), University of Washington
Comparative Histology (BioStr 505), University of Washington
Charles Holbrook Muller
Curriculum Vitae p. 8
Biology of Laboratory Animals (AnMed 521), University of Washington
Theoretical and Experimental Studies in Mammalian Reproduction (Psych 448), University of Washington
Modern Approaches to Classical Developmental Problems (Biol 586), University of Washington
Genetic Approaches to Human Disease (Gen 531), University of Washington 1996
Reproductive Toxicology (Envh 532), University of Washington 1996, 1998
Women in Science (Women 488), University of Washington 2003
TRAINEES
Ph.D. Trainees (Last 7 Years): Yasmeen Hussain (Biology)
Ph.D. Supervisor: (Member of Ph.D. Committee for 5 other students).
Chair of Committee for Stuart Ravnik, Department of Biological Structure; “Lipid transfer proteins of the
female reproductive tract and their role in sperm capacitation”; Ph.D. awarded 1991.
Chair of Committee for Hwan-wun Liu, Department of Biological Structure; “Identification and
characterization of two sperm maturation-related glycoproteins from mouse epididymis”; Ph.D. awarded
1988.
Other Trainees (Research Advisor for 15 additional students since 1984)
Residency / M.D. Fellowship Research
Robert Shapiro, (1994-1995)
Gregory Chen, (1995-1996)
Mitch Gold (1996-1997)
Greg Chow (2004-2006)
James Hotaling (2010-2012)
Natalya Lopushnyan (2011-2012)
Post-doctoral PhD Researchers – Visiting or Part-Time
Sharon Fernandez Edwards (2007-2008)
Dan Morgan (2008-2010)
Visiting Foreign Scientists
Jaime Sanchez-Cuenca, Valencia, Spain (1995)
Menaka Umapathy MD, South Africa (2007)
Undergraduate Independent Study: UROL 499, BIOL 499 or MICRO 499
Damon Thomas (1998)
Alison Lane (2002)
Kara Derickson (2004)
Korntika (Joyce) Srithananan, (2006)
Anastasia Davydova (2006)
Patipark Kueanjinda (2006)
Michalina Montano (2006-2007)
Kristina Winterholler (2007)
Harry Keller (2007)
Agnes Chin (2012)
Medical Student Independent Study MSRTP
Krister P Freese, SOM, Medical School MSRTP research project, Summer 2006- Spring 2007
Alexis Jaime, SOM, Medical School MSRTP research project, Summer 2009- Summer 2010
Eric Breeden, SOM, Medical School MSRTP research project, Summer 2009- Summer 2010
Jonathan Wang, SOM, Medical School MSRTP research project, Summer 2011 – Spring 2012
Christoph Reiner, SOM, Medical School MSRTP research project, Summer 2011
Graduate Student Independent Study
Judy Brown (1997-1999)
Charles Holbrook Muller
Curriculum Vitae p. 9
PROFESSIONAL DEVELOPMENT COURSES TAKEN
Faculty Workshop on Developing a Website for Courses, April 2006
Faculty Workshop on Teaching and Learning: Techniques for Effective Lecturing. September 8, 2004
LOCAL RESPONSIBILITIES (University of Washington)
Chair (2010-present), Member, Embryonic Stem Cell Research Oversight Committee, University of
Washington, May 2009 - present
Member, First Year Curriculum Committee, UW School of Medicine, Jan 2006 – 2010
Member, Medical School Prerequisites Committee, Sept. 2006 – 2007
Chair, WWAMI Histology Courses, Jan. 2006 - 2010
Member, Graduate Faculty, Department of Biological Structure, 1984-June 1991;
Ph.D. Supervisor (three Ph.D. students graduated) and Committee Member (five students)
Chair, Graduate Affairs Committee, Department of Biological Structure 1985-1987; Member, 1982-1987
Graduate Program Coordinator, Department of Biological Structure, 1985-1987
Chair, Ad Hoc Committee on Evaluating the Core Curriculum, Department of Biological Structure, 1986
Member, Developmental Biology Training Program, 1985-1991; Steering Committee, 1985-1987; Selection
Committee 1985-1987
Member, Molecular and Cellular Biology Training Program, 1985-1991
Member, Interdisciplinary Cellular and Molecular Biology Program, 1987-1991
NATIONAL and INTERNATIONAL RESPONSIBILITIES
AD HOC JOURNAL REFEREE
Andrology, Reproductive BioMedicine Online, Biology of Reproduction, Cell and Tissue Research,
Journal of Histochemistry and Cytochemistry, Fertility and Sterility, General and Comparative
Endocrinology, International Journal of Andrology, Journal of Andrology, Andrologia, Journal of
Experimental Zoology, Journal of Reproduction and Fertility, Obstetrics and Gynecology, Human
Reproduction, Journal of Infectious Diseases, Molecular Reproduction and Development, Journal of
Urology, European Journal of Obstetrics Gynecology & Reproduction and others
CONSULTANT, AVSC International and Family Health International, “A randomized controlled trial of two
vasectomy techniques”; Laboratory training and workshop, Kathmandu, Nepal, May-June, 1999, World
Health Organization Task Force on Methods for the Regulation of Male Fertility, Boston, 1985
REVIEWER, National Science Foundation (1987, 1989, 1990)
SITE VISITOR, NIH P30 Population Research Center, 1988
PARTICIPATING OBSERVER, Prevention of Infertility Workshop, Office of Technology Assessment,
United States Congress, Seattle, 1987
CHAIR, Local Arrangements Committee, American Society of Andrology, 2004-2006
Charles Holbrook Muller
Curriculum Vitae p. 10
PUBLICATIONS
Manuscripts Published in Refereed Journals
‡ Indicates Dr. Muller is senior or funded author.
* Denotes 5 most significant contributions.
1. Licht, P., C.H. Muller and H.W. Tsui 1976 Effects of mammalian and nonmammalian gonadotropins on
androgen production by minced rabbit testis. Biol. Reprod. 14:194-201.
2.‡ Muller, C.H. 1977 In vitro stimulation of 5-alpha-dihydrotestosterone and testosterone secretion from bullfrog
testis by nonmammalian and mammalian gonadotropins. Gen. Comp. Endocrinol. 33:109-121.
*3.‡ Muller, C.H. 1977 Plasma 5-alpha-dihydrotestosterone and testosterone in the bullfrog, Rana catesbeiana:
Stimulation by bullfrog LH. Gen. Comp. Endocrinol. 33:122-132.
4. ‡ Moore, F.L. and C.H. Muller 1977 Androgens and male mating behavior in rough skinned newts, Taricha
granulosa. Horm. Behav. 9:309-320.
5. Crews, D., V. Traina, F.T. Wetzel and C. Muller 1978 Hormonal control of male reproductive behavior in the
lizard, Anolis carolinensis: Role of testosterone, dihydrotestosterone, and estradiol. Endocrinology
103:1814-1821.
6. ‡ Moore, F.L., C.H. Muller and J.L. Specker 1979 Origin and regulation of plasma dihydrotestosterone and
testosterone in the rough-skinned newt, Taricha granulosa. Gen. Comp. Endocrinol. 38:451-456.
7. ‡ Muller, C.H. and P. Licht 1980 Gonadotropic specificity of androgen secretion by amphibian testes. Gen.
Comp. Endocrinol. 42:365-377.
8. Vernon, R.B., C.H. Muller, J.C. Herr, F.A. Feuchter and E.M. Eddy 1982 Epididymal secretion of a mouse
sperm surface component recognized by a monoclonal antibody. Biol. Reprod. 26:523-535.
9. Eddy, E.M., J.C. Herr, F.A. Feuchter, R.B. Vernon, C.H. Muller and B.A. Fenderson 1982 The heterogeneity
of the sperm surface as analyzed with monoclonal antibodies. Cell. Differen. 11:303-304.
10. ‡ Muller, C.H. and E.M. Eddy 1984 Localization of a sperm surface molecule in the epididymis. Ann. N.Y.
Acad. Sci. 438:533-534.
11. ‡ Stenchever, M.A., C.H. Muller, J.K. Huson, K.K. Shy and M.R. Soules 1985 Relationship of hamster ovum
sperm penetration assay to seminal fluid analyses in the evaluation of infertile couples. Arch. Androl.
14:65-72.
12. Eddy, E.M., C.H. Muller and C.A. Lingwood 1985 Preparation of monoclonal anti-sulfatoxygalactosylglycerolipid by in vitro immunization with a glycolipid-glass conjugate. J. Immunol. Meth. 81:137-146.
13. Eddy, E.M., R.B. Vernon, C.H. Muller, A.C. Hahnel and B.A. Fenderson 1985 Immunodissection of sperm
surface modifications during epididymal maturation. Am. J. Anat. 174:225-237.
14. Berger, R.E., C.H. Muller, M. Forster, D. Moore, R. McIntosh and B. Stewart 1986 Operative recovery of
vasal sperm from anejaculatory men: Preliminary report. J. Urol. 135:948-950.
15.‡ Muller, C.H. 1986 Precision, reliability and biological variability in human sperm function measured by
penetration of zona-free hamster oocytes. Int. J. Androl. (Suppl.) 6:31-41.
16. Kuzan, F.B., C.H. Muller, P.W. Zarutskie, L.L. Dixon and M.R. Soules 1987 Human sperm penetration assay
as an indicator of sperm function in human in vitro fertilization. Fertil. Steril. 48:282-286.
Charles Holbrook Muller
Curriculum Vitae p. 11
17. Vernon, R.B., C.H. Muller and E.M. Eddy 1987 Further characterization of a secreted epididymal
glycoprotein in mice that binds to sperm tails. J. Androl. 8:123-128.
18. Plymate, S.R., R.R. Nagao, C.H. Muller and C.A. Paulsen 1987 The use of sperm penetration assay in
evaluation of men with varicocele. Fertil. Steril. 47:680-683.
19. ‡ Fenderson, B.A., K. Toshimori, C.H. Muller, T.F. Lane and E.M. Eddy 1988 Identification of a protein in the
fibrous sheath of the sperm flagellum. Biol. Reprod. 38:345-357
20. ‡ Shy, K.K., M.A. Stenchever and C.H. Muller 1988 Sperm penetration assay and subsequent pregnancy: A
prospective study of 74 infertile men. Obstet. Gynecol. 71:685-690.
21. ‡ Ravnik, S. E., P.W. Zarutskie and C.H. Muller 1990 Lipid transfer activity in human follicular fluid:
Relationship to human sperm capacitation. J. Androl. 11:216-226.
22. Hillier, S.L., L.K. Rabe, C.H. Muller, P. Zarutskie, F.B. Kuzan and M.A. Stenchever 1990 Relationship of
bacteriologic characteristics and semen parameters among men attending an infertility clinic. Obstet.
Gynecol. 75:800-804.
23. Zarutskie, P.W., C.H. Muller, M. Magone and M.R. Soules 1989 The clinical relevance of sex selection
techniques. Fert. Steril. 52:891-905.
24. ‡ Liu, H.-W., J.-J. Wang, C.-F. Chao and C. Muller 1991 Identification of two maturation-related, WheatGerm-Lectin-binding proteins on the surface of mouse sperm. Acta Anat. 142:165-170.
25. ‡ Liu, H.-W., S.-T. Shang, C.-F. Chao and C. Muller 1991 The secretion of two sperm maturation-related
glycoproteins in BALB/c mouse epididymis. Cell Tiss. Res. 265:409-414.
26. ‡ Muller, C.H. 1992 The andrology laboratory in an Assisted Reproductive Technologies program: Quality
assurance and laboratory methodology. J. Androl. 13:349-360.
27. ‡ Liu, H.W., G.H. Sun, S.Y. Jeng and C. Muller 1992 Biosynthesis of two sperm maturation-related
glycoproteins in BALB/c mouse epididymis in vitro. Molec. Androl.
28. ‡ Ravnik, S. E., P.W. Zarutskie and C.H. Muller 1992 Purification and characterization of a human follicular
fluid lipid transfer protein which stimulates human sperm capacitation. Biol. Reprod. 47:1126-1133.
29. ‡ Ravnik, S. E., J.J. Albers and C.H. Muller 1993 A novel view of albumin-supported sperm capacitation: Role
of lipid transfer protein. Fertil. Steril. 59:629-638.
30. Cook, S.P., C.J. Brokaw, C.H. Muller and D.F. Babcock 1994 Sperm chemotaxis: Egg peptides control
cytosolic calcium to regulate flaggellar responses. Develop. Biol. 164:10-19.
31. Branigan, E.F. and C.H. Muller 1994 Efficacy of treatment and recurrence rate of leukocytospermia in
infertile men with prostatitis. Fertil. Steril. 62:580-584.
32. Branigan, E.F., L.R. Spadoni and C.H. Muller 1995 Identification and treatment of leukocytospermia in
couples with unexplained infertility. J. Reprod. Med. 40:625-629.
*33. ‡ Ravnik, S.E., J.J. Albers and C.H. Muller 1995 Stimulation of human sperm capacitation by purified lipid
transfer protein. J. Exp. Zool. 272:78-83.
Charles Holbrook Muller
Curriculum Vitae p. 12
34. Krieger, J.N., R.E. Berger, S.O. Ross, I. Rothman and C.H. Muller 1996 Seminal fluid findings in men with
nonbacterial prostatitis and prostatodynia. J. Androl. 17:310-318.
35. Alexander, B.H., H. Checkoway, C. van Netten, C.H. Muller, T.G. Ewers, J.D. Kaufman, B.A. Mueller, T.L.
Vaughan, and E.M. Faustman. 1996 Semen quality of men employed at a lead smelter. Occup. Environ.
Med. 53:411-416.
36. Berger, R.E., J.N. Krieger, I. Rothman, C.H. Muller, and S.L. Hillier 1997 Bacteria in the prostate tissue of
men with idiopathic prostatic inflammation. J. Urol. 157:863-865.
37. Rothman, I., R.E. Berger, P. Cummings, J. Jessen, C.H. Muller and W. Chapman 1997 Randomized clinical
trial of an absorbable stent for vasectomy reversal. J. Urol. 157: 1697-1700.
38. Berger, R.E., J.E. Miller, I. Rothman, J.N. Krieger, and C.H. Muller 1998 Bladder petechiae after cystoscopy
and hydrodistension in men diagnosed with prostate pain. J. Urol. 159: 83-85.
39.‡ Muller, C.H., R.W. Coombs and J.N.Krieger 1998 Effects of clinical stage and immunological status on
semen analysis results in Human Immunodeficiency Virus Type 1-seropositive men. Andrologia 30 (Suppl.
1): 15-22.
40. ‡ Shapiro, R.H., C.H. Muller, G.Chen and R.E. Berger 1998 Vasectomy reversal is associated with increased
reactive oxygen species production by seminal fluid leukocytes and sperm. J. Urol. 160:1341-6.
41. Alexander, B.H., H. Checkoway, E.M. Faustman, C. van Netten, C.H. Muller, and T.G. Ewers 1998
Contrasting associations of blood and semen lead concentrations with semen quality among lead smelter
workers. Amer. J Industr. Med. 34:464-469.
42. Coetzee, K., T.F. Kruger, C.J. Lombard, D. Shaughnessy, S. Oehninger, K. Özgür, K.O. Pomeroy, and C.
Muller 1999 Assessment of interlaboratory and intralaboratory sperm morphology readings with the use
of a Hamilton Thorne Research integrated visual optical system semen analyzer. Fertil. Steril. 71:80-84.
43. Branigan, E.F., M.A. Estes and C.H. Muller 1999 Advanced semen analysis: A simple screening test to
predict intrauterine insemination success. Fertil. Steril. 71:547-551..
44. Burton, K.A., B. Treash-Osio, C.H. Muller, E.L. Dunphy and G.S. McKnight 1999 Deletion of type IIα
regulatory subunit delocalizes protein kinase A in mouse sperm without affecting motility or fertilization.
J. Biol. Chem. 274 (34): 24131-136.
45. España, F., J. Sánchez-Cuenca, P.J. Fernández, J. Gilabert, A. Romeu, A. Estellés, M. Royo and C.H. Muller
1999 Inhibition of human sperm–zona-free hamster oocyte binding and penetration by Protein C Inhibitor.
Andrologia 31: 217-223.
*46. ‡ Muller, C.H. 2000 Rationale, interpretation, validation, and uses of sperm function tests. J. Androl. 21: 1030.
47.‡ Muller, C.H., R.E. Berger, L. Mohr and J.N.Krieger 2001 Comparison of microscopic methods for detecting
inflammation in expressed prostatic secretions. J. Urol. 166: 2518-2524.
48. Lee, J.C., C. H. Muller, I. Rothman, K.J. Agnew, D. Eschenbach, M.A. Ciol, J.A. Turner and R.E. Berger
2003 Prostate biopsy culture findings of men with chronic pelvic pain syndrome do not differ from those
of healthy controls. J. Urol 169: 584-588.
*49. Singh, N.P, C.H. Muller and R.E. Berger 2003 Effects of age on DNA double strand breaks and apoptosis in
human sperm. Fertil. Steril. 80:1420-1430. Cover photo credit.
Charles Holbrook Muller
Curriculum Vitae p. 13
50. Dunphy, E.J., J.C. Eickhoff, C.H. Muller, R.E. Berger and D.G. McNeel. 2004. Identification of antigenspecific IgG in sera from patients with chronic prostatitis. J. Clin. Immunol. 24:492-502.
51. Chow, G.E., C. Muller, E. Curnow, E. Hayes 2007 Expression of two-pore domain potassium channels in nonhuman primate sperm. Fertil. Steril. 87:397-404. Cover photo credit.
52. Amory, J.K, C.H. Muller, S.T. Page, E. Leifke, E.R. Pagel, A. Bhandari, B. Subramanyam, W. Bone, A.
Radlmaier and W.J. Bremner 2007 Miglustat Has No Apparent Effect on Spermatogenesis in Normal
Men. Hum. Reprod. 22:702-707 (Advanced Access Oct. 25, 2006).
53. Amory, J.K., J.W. Jessen, C. Muller, R.E. Berger 2010 Vasectomy by epithelial curettage without suture or
cautery: A pilot study in humans. Asian J Androl 12:315-321.
*54. Amory, J.K., C.H. Muller, J.A. Shimshoni, N. Isoherranen, Jisun Paik, Jan S. Moreb, D.W. Amory, R.
Evanoff, A.S. Goldstein and M.D. Griswold 2011 Suppression of Spermatogenesis by
Bisdichloroacetyldiamines is Mediated by Inhibition of Testicular Retinoic Acid Biosynthesis. J. Androl.
32:111-9. ePub 8/12/10. Cover photo credit.
55. Hotaling, J.M, J.F. Smith, M. Rosen, C.H. Muller and T.J. Walsh 2011 The relationship between isolated
teratozoospermia and clinical pregnancy after in vitro fertilization with or without intracytoplasmic sperm
injection: a systematic review and meta-analysis. .Fertil. Steril. 95:1141-5.
56. Graham, S.M., J.N. Kreiger, P.L.M. Githua, L.W. Wamuyu, S. Wale, K.M. Ramko, J.A. Dragavon, C.H.
Muller, S.E. Holte, K.N. Mandaliya, R.S. McClelland, N.M. Peshu, E.J. Sanders and R.W. Coombs 2011
Post-prostatic massage fluid/urine (post-PMF/U) as an alternative to semen for studying male genitourinary
HIV-1 shedding. Sex Transm Infect. 87:232-7. Epub 1/29/2011.
57. Morgan, D.J., C. H. Muller, N. Murataeva, B. Davis, K. Mackie. 2012 Delta-9-Tetrahydrocannabinol (∆9THC) attenuates mouse sperm motility and male fecundity. Brit J Pharmacol 165:2575-2583.
58. Hotaling, J.M., N.A. Lopushnyan, M. Davenport, H. Christianson, E. Pagel, C.H. Muller and T.J. Walsh 2013
Raw and test thaw semen parameters among men with newly diagnosed cancer. Fertil. Steril. 99:464-469.
59. Nya-Ngatchou JJ, S.L.M. Arnold, T.J. Walsh, C.H. Muller, S.T. Page, N. Isoherranen, and J.K. Amory. 2013
Intratesticular 13-cis Retinoic Acid is Reduced in Men with Abnormal Semen Analyses, a pilot study.
Andrology 1:325-331.
60. Mortimer, D., C.L.R. Barratt, L. Bjorndahl, C. de Jager, A.M. Jequier and C. H. Muller What should it take to
describe a substance or product as 'sperm-safe' Human Reproduction Update 2013 19:1-45.
61. Wang, J., C.H. Muller and K. Lin. 2013 Optimizing fertility preservation for pre- and post-pubertal males with
cancer. In: Current and Novel Medical Therapies for Male Infertility, J.C. Trussell, Ed. Semin Reprod Med
31:274–285.
Recently Submitted / In Preparation Manuscripts
Bar-Joseph, K.L., C.H. Muller, R.W. Coombs, E.A. Goecker, J.A. Dragavon, C. Gallardo, and B.S. Houmard 2013
Purification of human spermatozoa from seminal fluid spiked with cell-free and cell-associated Human
Immunodeficiency Virus Type-1 (HIV): Evaluation for intrauterine insemination in HIV discordant
couples.
Charles Holbrook Muller
Curriculum Vitae p. 14
Hotaling, J.M., C.H. Muller, E.R. Pagel, H Christianson and T.J. Walsh 2013 Modifiable factors that may improve
motile sperm yield during cryopreservation. Fertil. Steril.
Paik, J, M. Haenisch, C.H. Muller, A.S. Goldstein, S. Arnold, N. Isoherranen, T. Brabb, P.M. Treuting and J.
Amory 2014 Inhibition of retinoic acid biosynthesis by WIN 18,446 markedly suppresses spermatogenesis
and alters retinoid metabolism in mice. J. Biol. Chem.
Book Chapters, Web Sites and Training Programs
C1. Licht, P., H. Papkoff, S.W. Farmer, C.H. Muller, H.W. Tsui and D. Crews 1977 Evolution of gonadotropin
structure and function. Rec. Progr. Horm. Res. 33:169-248.
C2. ‡ Stenchever, M.A. and C.H. Muller 1986 Hamster penetration test and sperm fertilizing ability. In: C.M.
Fredricks, J. D. Paulson and A.H. DeCherney, Eds. Foundations of In Vitro Fertilization ; Washington
D.C., Hemisphere Publ. Corp. (Harper and Row); pp. 41-55.
C3. ‡ Muller, C.H. 1989 Germ Cell Development and Fertilization. In: H.D. Patton, A.F. Fuchs, B. Hille, A.M.
Scher and R.A. Steiner, Eds. Textbook of Physiology, Vol. 2, 21st ed., Philadelphia, W.B. Saunders Co.;
Chapter 69, pp. 1343-1379.
C4. ‡ Muller, C.H., Zarutskie, P.W., Stenchever, M.A. and Soules, M.R. 1990 The sperm penetration assay: One
of the best methods we have. Obstet. Gynecol. Report 2:412; 414-420.
C5. ‡ Muller, C.H. and Ravnik, S.E. 1995 Lipid Transfer Protein: A natural stimulator of the sperm capacitation
process. In: P. Fenichel and J. Parinaud, Eds. Human Sperm Acrosome Reaction, Paris, Colloque
INSERM; Vol. 236:67-84.
C6. ‡ Muller, C.H., A.R.Orkand and M.L. Astion 2001 Seminal Fluid. In: Body Fluids Tutor Seattle, WA;
Medical Training Software, University of Washington; http://www.medtraining.org/ltn/seminal/start.html.
C7.‡ Muller, C.H., S.A. Rothman, A. Reese, A.R. Orkand and M.L. Astion 2002 Semen analysis. Seattle, WA;
Medical Training Software, University of Washington.
C8. ‡ Muller, C.H. and E.R. Pagel 2013 Recovery, Isolation, Identification and Preparation of Spermatozoa from
Human Testis. In: Spermiogenesis and Spermatogenesis: Methods and Protocols (D.T. Carrell and K.I.
Aston, eds). Humana Press, New York, NY. Methods Mol Biol 927:227-240.
C9.
‡
Muller, C.H. T.K.Y. Lee and M.A. Montaño 2013 Improved Chemiluminescence Assay for Measuring
Antioxidant Capacity of Seminal Plasma. In: Spermiogenesis and Spermatogenesis: Methods and Protocols
(D.T. Carrell and K.I. Aston, eds). Humana Press, New York, NY. Methods Mol Biol 927:363-376.
C10. Wener, M.H., C.H. Muller and D.R. Dufour 2014 Male Genital Tract. In: Laboratory Medicine Diagnosis of
Disease in Clinical Laboratory, 2nd Ed. (M. Laposata, ed.) McGraw-Hill Medical, Burr Ridge, IL in press.
Other Publications (Not Peer-Reviewed)
O1. ‡ The Gypsy Moth: An integrated pest management approach. 1982. Gypsy Moth Task Force (Chip Muller,
Chair), Puget Sound Group, Sierra Club.
O2. ‡ Muller, C.H. 1986 Quality control. (Published presentation in The zona-free hamster oocyte penetration test
and the diagnosis of male infertility ; R.J. Aitken, ed.). Int. J. Androl. (Suppl.) 6:173-176.
Charles Holbrook Muller
Curriculum Vitae p. 15
O3. ‡ Muller, C.H. 1986 Induction of the acrosome reaction. (Published presentation in The zona-free hamster
oocyte penetration test and the diagnosis of male infertility ; R.J. Aitken, ed.). Int. J. Androl. (Suppl.)
6:158-162.
O4. ‡ Muller, C.H. 1986 Analysis of the patient population. (Published presentation in The zona-free hamster
oocyte penetration test and the diagnosis of male infertility ; R.J. Aitken, ed.). Int. J. Androl. (Suppl.)
6:182-183.
O5. ‡ Hacking, S.M. and C. Muller 1993 Exploring the rhododendron sanctuaries of Sikkim. J. Amer.
Rhododendron Soc. 47:182-188.
O6. ‡ Muller, C. and S.M. Hacking 1994 In Memoriam: George W. Muller. J. Amer. Rhododendron Soc. 48:7677.
O7. Worker reproductive health study: Preliminary report. April 11, 1994. Locals 480 and 9705, Cominco, Trail,
British Columbia.
O8. Worker reproductive health study: Final report. June 15, 1994. Locals 480 and Cominco, Trail, British
Columbia.
O9. ‡ Muller, C. and K. White 1996 Over the Doshong La: Exploring Kingdon-Ward’s ‘Rhododendron Fairyland’
in Southeastern Tibet. J. Amer. Rhododendron Soc. 50:146-153; 160.
Theses
T1. Muller, C.H. 1972 The effects of hypothalamo-adenohypophysial chorio-allantoic grafts on thyroids of
partially decapitated chick embryos. Master's Thesis, University of Colorado, Boulder. (Advisor: Professor
Bruce Criley).
T2. Muller, C.H. 1976 Steroidogenesis and spermatogenesis in the male bullfrog, Rana catesbeiana: Regulation
by purified bullfrog gonadotropins. Ph.D. Thesis, University of California, Berkeley. (Advisors: Professors
Paul Licht and Howard A. Bern).
Published Abstracts
A1. Muller, C.H. 1975 In vitro steroidogenesis by bullfrog testis. Proc. Soc. Study Reprod. Eighth Annual
Meeting, p. 95-96.
A2. Muller, C.H. 1976 Gonadotropin regulation of the bullfrog testis. Am. Zool. 16:259.
A3. Muller, C.H. 1976 Secretion of 5α-dihydrotestosterone by the bullfrog testis. Proc. Endocrine Soc. Progr.
Abst. 58th Annual Meeting, p. 268.
A4. Muller, C.H. and P. Licht 1978 Gonadotropin control of testicular functions in the anuran amphibian, Rana
pipiens. In: Comparative Endocrinology: Proceedings of the 8th International Symposium on Comparative
Endocrinology held in Amsterdam, The Netherlands, 19-23 June, 1978. P.J. Gaillard and H.H. Boer, Eds.,
Elsevier/North-Holland, New York, p.85.
A5. Muller, C.H. and P. Licht 1978 Secretion of androgens by amphibian testes in vitro: Effects of amphibian and
mammalian gonadotropins. Biol. Reprod. 18 (Suppl. 1):32.
Charles Holbrook Muller
Curriculum Vitae p. 16
A6. Muller, C.H. and P. Licht 1979 LH/FSH specificity of testicular androgen secretion in Amphibia. Am. Zool.
19:939.
A7. Vernon, R.B., C.H. Muller and E.M. Eddy 1981 Appearance of topographically restricted surface antigens on
sperm in the distal epididymis. Anat. Rec. 199:26A.
A8. Herr, J.C., C.H. Muller, B. Fenderson and E.M. Eddy 1981 Topographically restricted isoantigens of mouse
sperm present on spermatogenic cells. Anat. Rec. 199:113A.
A9. Muller, C.H., R.B. Vernon, F.A. Feuchter and E.M. Eddy 1981 Appearance and regulation of surface
components during sperm maturation in the mouse epididymis. Biol. Reprod. 24:44A.
A10. Muller, C.H. and E.M. Eddy 1983 Androgens regulate the appearance of sperm surface molecules
recognized by monoclonal antibodies. Endocrinology 112 (Suppl):249.
A11. Muller, C.H. and E.M. Eddy 1983 Androgen-dependence and properties of a mouse sperm surface
component defined by a monoclonal antibody. Biol. Reprod. 28 (Suppl 1):135.
A12. Stenchever, M.A., C.H. Muller and J. Huson 1984 Sperm penetration assay as related to semen analyses.
Arch. Androl. 12:133-134.
A13. Muller, C.H. 1984 Androgen dependent lectin-binding proteins in mouse epididymis. Anat. Rec. 208:122A123A.
A14. Muller, C.H. 1986 Sialylated polylactosamine glycoconjugate of the mouse epididymis and sperm surface.
Biol. Reprod. 34 (Suppl.1):216.
A15. Liu, H.-W. and C.H. Muller 1986 Maturation-related glycoproteins on the surface of epididymal
spermatozoa in the mouse. Biol. Reprod. 34 (Suppl.1):217.
A16. Berger R.E., D. Moore, J. Rajfer, M. Bustollo, and C. Muller. 1986 Vasotomy: A treatment for
anejaculation. Presented at Annual Meeting of the Northwest Urological Society, Tacoma, Washington.
A17. Berger RE, C. Muller, R. MacIntosh, and D. Moore. 1986 Operative recovery and insemination of vasal
sperm: A new and repeatable procedure for the treatment of the anejaculatory infertile male. (Presented at
the Annual American Urological Association Meeting Atlanta 1985.) J. Urol. 135:948-950.
A18. Shy, K.K., M.A. Stenchever, C.H. Mueller, R.E. Berger and B. Erickson 1986 Cumulative pregnancy rates
in spouses of infertile men treated with doxycycline after an abnormal sperm penetration assay (SPA).
Presented at ACOG District VII/IX Annual Meeting, November 2-6, 1986, Maui, Hawaii.
A19. Liu, H.-W. and C.H. Muller 1987 Maturation-related glycoproteins in mouse epididymis. J. Androl. 8:46P.
A20. Muller, C.H. 1987 Polylactosamine on the mouse sperm surface during maturation and capacitation. J.
Androl. 8:40P.
A21. Liu, H.-W. and C.H. Muller 1987 Apocrine secretion in mouse epididymis. Anat. Rec. 218:79A.
A22. Liu, H.-W. and C.H. Muller 1987 Synthesis of maturation-related proteins in the mouse epididymis. Biol.
Reprod. 36 (Suppl. 1):69.
A23. Plymate, S.R., C.A. Paulsen and C.H. Muller 1987 Studies of testicular function following varicocele
ligation. Program and Abstracts, American Fertility Society, p. 88.
Charles Holbrook Muller
Curriculum Vitae p. 17
A24. Ravnik, S. and C.H. Muller 1988 Human sperm capacitation and lipid transfer proteins from female
reproductive tract. J. Androl. 9:P41.
A25. Muller, C.H. 1988 Mouse sperm fertilizing ability is modified by an epididymal polylactosamine-containing
glycoprotein. Biol. Reprod. 38 (Suppl.1):143.
A26. Ravnik, S. E. and C.H. Muller 1988 Partial purification of lipid transfer activity from female reproductive
tract and its role in sperm capacitation. Biol. Reprod. 38 (Suppl.1):60 .
A27. Muller, C.H., S.C. Jeffay, S. M. Harvey and P.W. Zarutskie 1989 Sperm capacitation time differs among
fertile men, men from infertile couples and men with abnormal semen analyses. J. Androl. 10:P35.
A28. Ravnik, S. E. and C.H. Muller 1989 Relationship between support of human sperm capacitation and lipid
transfer activity among different albumin preparations. J. Androl. 10:P21.
A29. Ravnik, S. E., P.W. Zarutskie and C.H. Muller 1990 Purification of a lipid transfer protein from human
follicular fluid that stimulates human sperm capacitation. In: Fertilization in Mammals (B. Bavister, J.
Cummins, E.R.S. Roldan, eds.) Boston, Serono Symposia, USA, p. 433.
A30. Ravnik, S. E., P.W. Zarutskie and C.H. Muller 1990 Serum inhibits follicular fluid-induced sperm
capacitation. J. Androl. 11:P27.
A31. Muller, C.H., S.E. Ravnik and P.W. Zarutskie 1990 Follicular fluid enhanced sperm penetration assay: Use
in diagnosis of sperm dysfunction. J. Androl. 11:42P
A32. Schnarsky, K.E., C.H. Muller and P.W. Zarutskie 1990 The effect of follicular fluid pretreatment of sperm
on oocyte fertilization rates in the in vitro fertilization program. Fertil. Steril. (Program Suppl. 38th Annual
Meeting of the Pacific Coast Fertility Society):A11.
A33. Zarutskie, P.W., L. Dixon, C.H. Muller and M.A. Stenchever 1990 The predictive role of enhanced sperm
penetration assay on fertilization rates in in vitro fertilization (IVF) microinjection. Abstacts and Program,
VII World Congress on Human Reproduction, Helsinki.
A34. Ravnik, S.E., G.J. Killian and C.H. Muller 1990 Bovine reproductive fluids have lipid transfer protein and
stimulate human sperm capacitation. Biol. Reprod. 42 (Suppl.1):86.
A35. Muller, C.H., P.W. Zarutskie, M.A. Stenchever, D.E. Moore, L.R. Spadoni and M.R. Soules 1990 Enhanced
sperm penetration and zona binding assays identify new categories of sperm dysfunctions in
“idiopathically” infertile men. Amer. Fertility Soc. 46th Annual Meeting Program Suppl.:S14.
A36. Ravnik, S. E. and C.H. Muller 1991 Stimulation of human sperm capacitation in protein-free medium by
lipid transfer protein. J. Androl. (Suppl):P27.
A37. Branigan, E.F. , P.W. Zarutskie and C.H. Muller 1992 Efficacy of identifying and treating leukocytospermia
in couples with idiopathic infertility. Fertil. Steril. (Program Suppl. 40th Annual Meeting of the Pacific
Coast Fertility Society):A9.
A38. Muller, C.H. and P.W. Zarutskie 1992 Prediction and diagnosis of human in vitro fertilization success and
failure by enhanced sperm penetration and sperm-zona binding assays. Amer. Fertility Soc. 48th Annual
Meeting Program Suppl.:S105. [Award-winning poster]
A39. Branigan, E.F. and C.H. Muller 1993 Efficacy of treatment and recurrence rate of leukocytospermia in
infertile men. Fertil. Steril. (Program Suppl. 41st Annual Meeting of the Pacific Coast Fertility Society).
Charles Holbrook Muller
Curriculum Vitae p. 18
A40. Cook. S.P., C.J. Brokaw, C.H. Muller and D.F. Babcock 1993 A unifying model for sperm chemotaxis to
egg peptides. FASEB J. 7:A1248.
A41. Ewers, T.G., C.H. Muller, B. Alexander, H. Checkoway, J.S. Woods and E.M. Faustman. 1993 Examination
of the effects of lead on sperm chromatin structure and spermatogenesis in rats. Program and Abstracts,
Pacific Northwest Assoc. Toxicologists 1993 Ann. Meeting.
A42. Branigan, E.F., M.A. Estes and C.H. Muller 1994 Extensive sperm testing can help identify men with severe
male factor that can be treated with modified conventional IVF, and those that need intracytoplasmic sperm
injection (ICSI). Program Suppl. 42nd Annual Meeting of the Pacific Coast Fertility Society:A12.
A43. Branigan, E.F., M.A. Estes and C.H. Muller 1994 Identification and quantification of leukocytes in prostate
secretion and semen of fertile and infertile men by monoclonal antibodies. Program Suppl. 42nd Annual
Meeting of the Pacific Coast Fertility Society:A24.
A44. Muller, C.H. S.J. Klebanoff and R.E. Berger 1994 Human sperm responses to activated neutrophils and
pentoxifylline. Program and Abstr. Western Section Amer. Urological Assoc. Ann. Meeting, p.2.
A45. Alexander, B.H., H. Checkoway, E.M. Faustman, C. van Netten, C.H. Muller and T.G. Ewers 1994 Effects
of lead exposure on male reproductive health indicators among lead smelter workers. Program and
Abstracts International Symposium of Epidemiology in Occupational Health.
A46. Muller, C.H., H. Checkoway, B.H. Alexander, C. van Netten, T.G. Ewers and E. Faustman 1995
Relationship between metal exposure and sperm acrosomal function tests among workers at a lead smelter.
J. Androl. (Suppl):P42.
A47. Alexander, B.H., H. Checkoway, C. van Netten, C.H. Muller, T.G. Ewers and E. Faustman 1995 Semen
quality of men employed at a lead smelter. Abstracts Int’l. Congr. Toxicology VIII:76-PD-6.
A48. Muller, C.H., H. Checkoway, B.H. Alexander, C. van Netten, T.G. Ewers and E. Faustman 1995 Sperm
function and motility tests among workers at a lead smelter. Abstracts Int’l. Congr. Toxicology VIII:76PD-7.
A49. Kreiger, J.N., R.W. Coombs, C. Muller, S.O. Ross, C. Speck, T. Hooton and L. Corey. 1995 Cellular
immune response in semen of HIV seropositive men: Comparison with systemic immunity. Annual
Meeting Infectious Diseases Society of America, San Francisco, CA, September 1995.
A50. Chen, G.L., C. Muller and R.E. Berger 1995 Vasal aspiration of sperm. Amer. Urol. Assoc. April 23-28,
Las Vegas. J. Urol. 153 (Suppl): 261A.
A51. Eberhardt, K.B., D.E. Moore, V.Y. Fujimoto, E.F. Branigan, P.W. Zarutskie and C.H. Muller 1995 The
follicular fluid hamster sperm penetration assay predicts in vitro fertilization. Amer. Fertility Soc. 51st
Annual Meeting Program Suppl.:S102-103.
A52. Chen, G.L., C. Muller and R.E. Berger 1995 Cadaveric orchiectomy as a technique for harvesting viable
sperm. Program and Abstr. Western Section Amer. Urological Assoc. 71st Ann. Meeting, p.74.
A53. Shapiro, R. H., C.H. Muller and R.E. Berger 1995 The presence of reactive oxygen species in the semen of
men after vasectomy reversal. Northwest Urological Society 42nd Annual Meeting Program and
Abstracts:26.
A54. Muller, C.H. 1995 Advanced sperm tests re-define idiopathic male fertility. Northwest Urological Society
42nd Annual Meeting Program and Abstracts:27.
Charles Holbrook Muller
Curriculum Vitae p. 19
A55. Muller, C.H., J.N. Kreiger, S. Ross, C. Speck and R.W. Coombs 1996 Seminal WBC subtypes and semen
analysis in HIV-positive men. HIV Shedding in Semen: Research Issues Workshop. Division of Urology
(NIDDK), National Institutes of Health, Bethesda, MD, February 1996.
A56. Shapiro, R.H., C.H. Muller and R.E. Berger 1996 The presence of reactive oxygen species in the semen of
men of proven fertility and after vasectomy reversal. American Urological Association Annual Meeting
Program and Abstracts. J. Urol. 155 (Suppl.):478A.
A57. Rothman, I., R.E.Berger, J. Jessen, C.H. Muller, and W. Chapman 1996 Randomized trial of two-layer
vasovasostomy vs. Vasostent® vasovasostomy: Long term follow-up and pregnancy. American Urological
Association Annual Meeting Program and Abstracts. J. Urol. 155 (Suppl.):363A.
A58. Berger, R.E., J.N. Krieger, S. Ross, I. Rothman, C. Muller, and S. Hillier 1996 “Non-bacterial” prostatitis: Is
it caused by bacteria? American Urological Association Annual Meeting Program and Abstracts. J. Urol.
155 (Suppl.):675A.
A59. Branigan, E.F., M.A. Estes and C.H. Muller 1996 Distribution of leukocytes in the male genital tract and
their effect on fertility. Program Suppl. 44th Annual Meeting Pacific Coast Fertility Society:A3.
A60. Branigan, E.F., M.A. Estes and C.H. Muller 1996 Treatment of persistent leukocytospermia with
pentoxifylline-deoxyadenosine Percoll gradient intrauterine insemination. Program Suppl. 44th Annual
Meeting Pacific Coast Fertility Society:A20.
A61. Muller, C.H., R.E. Berger, S.O. Ross, I. Rothman and J.N. Krieger 1996 Seminal fluid findings in men with
non-bacterial prostatitis or prostatodynia. J. Androl. (Suppl):P52.
A62. Alexander, B.H., H. Checkoway, C. van Netten, C.H. Muller, T.G. Ewers and E. Faustman 1996 The
relationship of blood and semen lead concentrations to semen quality parameters among lead smelter
workers. Soc. Epidemiologic Res. 29th Ann Mtg., June 12-15, Boston. Amer. J. Epidemiol. 143:67.
A63. Muller, C.H., L. Mohr, R.E. Berger and J.N.Krieger 1997 Evaluation of microscopic methods for detection
of inflamed prostatic secretion. Amer. Urol. Assoc. April 12-17, New Orleans. J. Urol. 157 (Suppl):241.
A64. Muller, C.H., R.H. Shapiro, G. Chen and R.E. Berger 1998 Effects of years of vasectomy, time post-surgery
and fertility on reactive oxygen species generated by seminal leukocytes and sperm of men after vasectomy
reversal. J. Androl. (Suppl): 27.
A65. Branigan, E.F., M.A. Estes and C.H. Muller 1998 Advanced semen analysis: A simple screening test to
predict intrauterine insemination (IUI) success. Program Suppl. 46th Annual Meeting Pacific Coast
Fertility Society:A3. [First place poster award].
A66. Muller, C.H., E.F. Branigan, M.A. Estes and R.E. Berger 1998 Effectiveness of pentoxifylline +
deoxyadenosine for improving sperm motility and recovery from semen of men with sperm motility
abnormalities. Amer. Fertility Soc. 54th Annual Meeting / IFFS ‘98 Program Suppl.: S274.
A67. Muller, C.H. and R.E. Berger 2000 Characteristics of seminal fluid leukocytes in men with and without
chronic pelvic pain syndrome. J. Androl. (Suppl): 68.
A68. Muller, C.H., D. Diamond and R.E. Berger 2002 Protein profiling of expressed prostatic secretions. J.
Androl. (Suppl): 89.
A69. Singh, N.P, C.H. Muller and R.E. Berger 2002 DNA double strand breaks and apoptosis in human sperm:
Effects of donor age. Amer. Soc. Reprod. Med. 58th Annual Meeting 2002 Progr. Suppl.: O-181.
Charles Holbrook Muller
Curriculum Vitae p. 20
A70. Chow, G.E., E. Hayes, C. Muller, E. Curnow 2005 Expression and function of two-pore domain potassium
channels in non-human primate sperm. Fertil. Steril. 84 (Suppl. 1): S6.
A71. Freese, K., C. Muller, E. Pagel, C. Yang and R. Berger 2007 Cytokines MCP-1, MIF and ENA-78 in seminal
plasma: Relationships with inflammation and chronic pelvic pain syndrome. Amer. Soc. Andrology. J
Androl Mar/Apr 2007 Suppl p.61.
A72. Morgan, D.J., C. H. Muller, N.A. Murateava, S.E. Hastings and K. Mackie 2008 Temporal dynamics of
cannabinoids on sperm ATP levels and basal motility. Amer. Soc. Cell Biol., 48th Annual Meeting.
A73. Amory, J.K., J.W. Jessen, C. Muller, W.J. Bremner and R.E. Berger 2009 Vasectomy by epithelial curettage
without suture or cautery: A pilot study in man. Amer. Soc. Andrology, J. Androl. Mar/Apr 2009 Suppl. p
48.
A74. Amory J., C. Muller, D. Amory, M. Griswold, C. Small, A. Goldstein and W. Bremner 2010 Reversible
Suppression of Spermatogenesis by the Bis-dichloroacetyldiamine WIN 18,446 is Mediated by Inhibition
of Retinoic Acid Activity within the Testes. J. Androl. Mar/Apr 2010 Supplement p. 77.
A75. Hotaling, J.M., C.H. Muller, E.R. Pagel, H Christianson and T.J. Walsh 2010 Modifiable factors that may
improve motile sperm yield during cryopreservation. 2010 Amer Soc Reprod Med
A76. Bar-Joseph, K.L., C.H. Muller, J.A. Dragavon, C. Gallardo, R.W. Coombs and B.S. Houmard 2011 Semen
washing protocol for intrauterine insemination in HIV discordant couples – A pilot study. 2011 Pacific
Coast Reproductive Society.
A77. Lopushnyan, N.A., J.M. Hotaling, E. Pagel, H. Christianson, C.H. Muller, and T.J. Walsh 2011 Predictors of
sperm recovery after cryopreservation among men with testis cancer. 2011 AUA
A78. Hotaling, J.M., C.H. Muller, E.R. Pagel, H Christianson and T.J. Walsh 2011 Cryopreservation with prokinetic agents may have an adverse impact on sperm motility and recovery in men with testis cancer. Am
Soc Reprod Med Fertil. Steril. 96 (3)
INVITED SYMPOSIUM or RESEARCH LECTURES
2010
2009
2006
2005
2002
2001
1999
1998
1998
“Sperm Tales: How Disease and Environment Affect Fertility and the Next Generation”, Physiologic
Society of Southern Africa, Annual Meeting Key-Note Address, East London, South Africa, Sept 27-29.
“Leukocytes and Spermatocytes: Seminal Clues to Reproductive Health”, Laboratory Science Forum,
American Society of Andrology, Philadelphia PA April 2009.
“Adventures in Vasectomy / Semen Analysis in Nepal” Laboratory Science Forum, American Society of
Andrology Annual Meeting, Chicago Il April 8, 2006.
“Quality Sperm for Quality Embryos: Sperm Do Matter”, College of Reproductive Biology, American
Association of Bioanalysts, Tampa, FL., May 2005.
“Radicals and Comets: Roles of Reactive Oxygen Species in Prostatitis and Inflammation”, Dept. of
Urology, University of Washington.
“Semen Parameters in Vasectomy Research – What Do They Mean?” Expert Consultation on Vasectomy
Effectiveness, Family Health International and EngenderHealth, Durham, N.C., April 18-19.
“Current Sperm Function Testing”, American Society of Andrology Laboratory Workshop Course
“Andrology Testing - Separating the Wheat from the Chaff", Louisville KY, April 13.
“Reactive Oxygen Species and Male Infertility”, Dept. of Urology, University of Washington.
“Methods in Semen Analysis: Pitfalls, Advances and Interpretation” SmithKline Beecham Clinical
Laboratories, Sixth Annual Fall Symposium, Seattle, WA, Sept. 26.
Charles Holbrook Muller
Curriculum Vitae p. 21
1996
1996
1995
1994
1993
1993
1993
1993
1992
1991
1991
1990
1989
1989
1989
1988
1987
1986
1988
1986
1986
“Semen Analysis and White Blood Cell Subtypes in HIV Infection”, NIH Workshop on HIV and Semen,
Feb. 1-2.
“Lipid Transfer Protein Regulation of Human Sperm Capacitation”, Dept. of Anatomy, Georgetown Univ.
“Capacitation: Role of Lipid Transfer Protein” International Symposium on the Human Sperm Acrosome
Reaction; Collioure, France, Sept. 6-9.
“Diagnostic Value of Sperm Function Tests in Male Infertility”, Dept. of Urology, Univ. of Washington.
“Tests of Sperm Function; Quality Control and Quality Assurance”, American Fertility Society
Postgraduate Course IV: Technological Advances in the Diagnosis and Management of Male Infertility
(Larry I. Lipshultz, MD, Program Director), October 9.
“Sperm Function Tests: Do They Provide Relevant Information Regarding Male Fertility? PRO
Argument”, American Fertility Society Postgraduate Course IX: Controversies in Reproductive Biology
and Technology (Jeffrey P. Boldt, PhD and Jeffrey V. May, PhD, Course Directors), October 9.
"Sperm Function Tests: Diagnostic and Predictive Values", Dept Urology Grand Rounds, Beth Israel
Medical Center; and Dept. Obstetrics and Gynecology, Roosevelt/St. Lukes Hospitals, New York, NY.
"Therapy - Related Diagnoses of Sperm Dysfunctions in Male Infertility", New York Society of
Reproductive Medicine, New York, NY, January 20.
“The Andrology Laboratory in an ART Program: Quality Assurance and Laboratory Methodology”,
American Society of Andrology Postgraduate Course "In Vitro Fertilization: Scientific Foundation and
Clinical Application", Bethesda, March 27.
“Sperm Function Assays: Present and Future”, American Fertility Soc. Annual Meeting, Orlando, October.
“Sperm Function Tests: Diagnostic and Predictive Value”, UW Dept Ob/Gyn Grand Rounds.
“Sperm Dysfunctions in “Idiopathically” Infertile Men, and How the Female Controls Fertilization”, UW
Dept Biological Structure; “How Does Human Follicular Fluid Stimulate the Fertilizing Ability of
Sperm?” UW Dept Ob/Gyn Grand Rounds.
"A New Rationale for Diagnosis and Treatment of Human Sperm Dysfunction in Idiopathic Male
Infertility", Dept Anatomy and Reproductive Biology, Univ. of Hawaii; “Post-Translational Modification
of an Epididymal Glycoprotein Alters Sperm Fertilizing Ability”, UW Population Center for Research in
Reproduction; “Fertilizing Ability of Spermatozoa is Enhanced by Reproductive Tract Secreted Proteins”,
UW Dept Ob/Gyn Grand Rounds; “New Methods of Assessing Human Sperm Dysfunction”, UW
Population Center for Research in Reproduction.
“The Diagnostic Relevance of the Hamster Egg Test”, American Fertility Society Annual Meeting, San
Francisco, November.
"Regulation of Sperm Fertilizing Ability by Modification of an Epididymal Glycoprotein", Serono
Symposium on Fertilization in Mammals, in honor of Ryuzo Yanagimachi, Boston, August.
"Sperm Surface Molecules Modulating Fertilization in the Mouse", UW Department of Biological
Structure; "Sperm Fertility is Both Activated and Suppressed During Maturation in the Epididymis", UW
Developmental Biology Training Program; "Sperm Surface Molecules Involved in Fertilization in the
Mouse", UW Population Center for Research in Reproduction; "Male Infertility: Diagnosis of Sperm
Capacitation and Acrosome Reaction Defects, and Regulation by Extracellular Ions", UW Dept Ob/Gyn
Grand Rounds.
"Mouse Sperm Surface Molecule Involved in Fertilization", Department of Anatomy and Cell Biology,
University of Virginia; and Department of Anatomy and Reproductive Biology, Georgetown University,
and The Oregon Regional Primate Center.
"New Developments in Male Infertility", Regional Reproductive Endocrinology Course, Bellingham, WA
"Molecular Mechanisms and Defects of Sperm Fertilizing Ability", UW Dept Ob/Gyn Grand Rounds;
"Molecular Changes in the Surface of Mouse Sperm Prior to Fertilization", UW Dept Biological Structure,
University of Washington.
"Update on Laboratory Diagnosis of Male Infertility." Workshop at American Fertility Society Annual
Meeting, Atlanta, October.
"Epididymal Molecule Involved in Sperm Maturation in the Mouse." Presented at the International
Symposium on Recent Advances in Fertility Regulation (Sponsored by World Health Organization and
Ministry of Public Health, People's Republic of China) Hangzhou, China, April 30.
"Differentiation of the Sperm Plasma Membrane: Sperm Maturation." Presented at the Gamete Physiology
Symposium, Society for the Study of Reproduction, Ithaca, New York, July 15.
Charles Holbrook Muller
Curriculum Vitae p. 22
1985
1983
"Stimulation of Acrosome Reaction" and "Quality Control", World Health Organization, Male Task Force,
Boston, MA.; "Infertile Men with 'Normal' Sperm: Prospects for Evaluation and Therapy", UW Dept
Ob/Gyn Grand Rounds.
"Regulation of Male Germ Cell Differentiation" Department of Zoology, University of Washington;
"Properties of Sperm Surface Molecules Recognized by Monoclonal Antibodies", Department of
Biochemistry, University of Toronto and the Hospital for Sick Children, Toronto, Ontario; "AndrogenDependent Sperm Surface Molecule Recognized by a Monoclonal Antibody", Department of Anatomy,
University of Virginia.