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CD-8241B
June 2001
Beckman
Coulter
Capillary Electrophoresis
Supplies, Accessories & Options
PUTTING CE TO WORK
CHIRAL
A N A LYS I S
Contents
Index
Navigation & Information
D RU G
SCREENING
C A R B O H Y D R AT E
A N A LYS I S
P ROT E I N
C H A R AC T E R I Z AT I O N
GENETIC
A N A LYS I S
B E C K M A N C O U LT E R
Capillary Electrophoresis
B
eckman Instruments, Inc., was founded by Dr. Arnold O. Beckman in 1935
with the invention of the pH meter, a project initiated from a colleague’s
need to monitor the acidity of citrus juice. Since then, Beckman Coulter
has grown into a 1.8 billion-dollar corporation with many scientific firsts to its
credit. Commercial electrophoresis systems were first introduced by Beckman in
1953 with the development of the Model H, a Tiselius cell with Schlieren photography
and advanced optics. Since that time electrophoresis has played an important role
in Beckman’s bioresearch and clinical diagnostic developments.
Beckman’s contributions to capillary electrophoresis (CE) began in 1989
with the launch of the P/ACE 2000. This instrumentation boasted many CE firsts
including a cartridge design with liquid thermostating of the capillary, micropreparative fraction collection and automated personal computer control. Since
the introduction of the P/ACE, Beckman Coulter has been the acknowledged leader
in the development of CE technology with advancements in hardware, software
and chemistry. These advancements included laser-induced
fluorescence, diode-array detection, independent sample and buffer temperature
control, advanced CE data reduction and a myriad of CE chemistries.
These advancements soon moved from generic CE to applied instrumentation
first seen with the release of the Paragon CZE 2000, a multi-capillary system for
automated serum protein analysis in clinical diagnostics. This was soon followed by
the P/ACE MDQ, a rugged line of instrumentation for pharmaceutical/biotechnology
methods development and quality control. The CEQ 2000XL, a multi-capillary fully
automated DNA Sequencing System, the dual-wavelength P/ACE MDQ LIF detection
system, and the P/ACE MDQ-LCQ CE-MS/MS system are the newest additions.
Beckman Coulter continues to lead the field of capillary electrophoresis,
strongly committed to further CE research and development. Our direction is guided
by you, to apply this core technology to systems providing key analytical solutions.
Beckman Coulter
Contents
Index
2
1989
1990
1991
P/ACE
Introduction
Improved UV
Detection
Gold Software
for CE
eCAP ssDNA
100 Kit
B E C K M A N C O U LT E R
I N N OVAT I O N S
1992
IN
C APILLARY
Laser-Induced
Fluorescence
Detection
Molecular
Weight Software
CE-MS Adapter
eCAP SDS 200
Protein Kit
Sample Cooling
Isoelectric
Focusing
Capability
Enhanced
Sensitivity
Cartridge
Diode Array
Detection
P/ACE 5000
eCAP SDS
14-200
Protein Kit
eCAP dsDNA
20,000 Kit
eCAP cIEF
3-10 Kit
635 Laser
Module
The Chiral
Solution
Paragon CZE
2000 Serum
Protein Analyzer
P/ACE MDQ
Quality Control
System
P/ACE MDQ
Methods
Development
System
P/ACE MDQ
Chiral Analysis
System
P/ACE 5000
Series Education
System
MDQ Kits
APTS
Monosaccharide
Grade
P/ACE MDQ
Dual λ LIF
P/ACE MDQ
Glycoprotein
System
P/ACE MDQ
Carbohydrate
System
P/ACE MDQ
DNA System
32 Karat
Software
Version 4.0
CEQ 2000XL
Longer Faster
Reads
CEQ Chem Kit
DTCS – Quick
Start Chemistries
CEQ 2000XL
Automated
Heterozygote
Detection
eCAP
Carbohydrate
Labeling Kit
1993
ELECTROPHORESIS
eCAP dsDNA
1000 Kit
1994
eCAP Amine
Capillary Methods
Development Kit–
Small Molecules
1995
eCAP Amine
Capillary Methods
Development Kit–
Proteins
eCAP Neutral
Capillary Methods
Development
Kit/Proteins
eCAP Chiral
Methods
Development Kit
1997
1996
eCAP N-Linked
Oligosaccharide
Profiling Kit
LIFluor
dsDNA 1000 Kit
eCAP ssDNA
100-R Kit
The
Carbohydrate
Solution
The DNA
Solution
P/ACE Station
Software
P/ACE
MDQ
1999
1998
NH2-
-O3S
-O3S
SO3APTS
Highly Sulfated
Cyclodextrins
HS CD
External
Detector Adapter
1.6 Software
Enhancement
CEQ 2000
DNA Sequencer
2000
P/ACE
MDQ-LCQ
CE-MS/MS
System
Beckman Coulter
Contents
Index
3
CAPILLARY ELECTROPHORESIS
A SIMPLE TECHNIQUE
Capillary electrophoresis (CE) is a family of related
separation techniques that use narrow-bore fusedsilica capillaries to separate a complex array of
large and small molecules. High voltages are used
to separate molecules based on differences in charge,
size and hydrophobicity. Injection into the capillary is
accomplished by immersing the end of the capillary
into a sample vial and applying pressure, vacuum
or voltage. Depending on the types of capillary and
buffers, CE can be segmented into several separation
techniques. Examples of these include:
CAPILLARY ZONE ELECTROPHORESIS (CZE), also known as
free-solution CE (FSCE), is the simplest form of CE.
The separation mechanism is based on differences in
the charge-to-mass ratio of the analytes. Fundamental
to CZE are homogeneity of the buffer solution and
constant field strength throughout the length of the
capillary. The separation relies principally on the pHcontrolled dissociation of acidic groups on the solute
or the protonation of basic functions on the solute.
CAPILLARY GEL ELECTROPHORESIS (CGE) is the adaptation
of traditional gel electrophoresis into the capillary
using polymers in solution as a molecular sieve.
This allows solutes having similar charge-to-mass
ratios to be resolved by size.
CAPILLARY ISOELECTRIC FOCUSING (CIEF) allows amphoteric
molecules, such as proteins, to be separated by
electrophoresis in a pH gradient generated between
the cathode and anode. A solute will migrate to a
point where its net charge is zero. At this isoelectric
point (the solute’s pI), migration stops and the sample
is focused into a tight zone. In cIEF, once a solute
has focused at its pI, the zone is mobilized past the
detector by either pressure or chemical means.
ISOTACHOPHORESIS (ITP) is a focusing technique based on the migration of the sample components between leading and terminating electrolytes. Solutes having mobilities intermediate to those of the leading and terminating electrolytes stack into sharp, focused zones.
Although it is used as a mode of separation, transient ITP has been
used primarily as a concentration technique.
ELECTROKINETIC CHROMATOGRAPHY (EKC) is a family of electrophoresis
techniques named after electrokinetic phenomena, which include
electroosmosis, electrophoresis and chromatography. A key example of this is seen with cyclodextrin-mediated EKC. Here the differential interaction of enantiomers with the cyclodextrins in combination with an electrophoretic field allows for the separation of chiral
compounds.
MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY (MECC OR MEKC)
is a mode of electrokinetic chromatography in which surfactants are
added to the buffer solution at concentrations which form micelles.
The separation principle of MEKC is based on a differential partition
between the micelle and the solvent. This principle can be employed
with charged or neutral solutes and may involve stationary or mobile
micelles. MEKC has great utility in separating mixtures which
contain both ionic and neutral solutes.
CAPILLARY ELECTROCHROMATOGRAPHY (CEC) is a hybrid separation
method that couples the high separation efficiency of CZE with
HPLC, and uses an electric field rather than hydraulic pressure to
propel the mobile phase through a packed bed. Since there is minimal backpressure, it is possible to use small-diameter packings and
achieve very high efficiencies.
NON-AQUEOUS CAPILLARY ELECTROPHORESIS (NACE) involves the separation of analytes in a medium composed of organic solvents. The
viscosity and dielectric constants of organic solvents affect both
sample ion mobility and the level of electroosmotic flow. The use
of non-aqueous medium allows additional selectivity options in
methods development and is also valuable for the separation of waterinsoluble compounds.
`
Summary
Block diagram
of basic CE system.
The magnified view depicts
the capillary interior with molecules
of different size and charge separating. As
illustrated, in free-solution capillary electrophoresis, the
positive molecules migrate toward the negative electrode
while the electroosmotic flow carries the neutral and
negatively charged molecules in the same direction.
Beckman Coulter
Contents
The large selection of separation mechanisms combined with its
“in solution” approach has allowed capillary electrophoresis to
excel where many analytical techniques have failed. CE has been
successful primarily with complex hydrophilic macromolecules and
highly charged solutes. These techniques have led to the rapid
separation and quantitation of:
• Nucleic Acids
• Proteins
• Glycoproteins
• Peptides
• Carbohydrates
• Basic Pharmaceuticals
• Chiral Isomers
• Inorganic and Organic Ions
Index
4
THE BECKMAN COULTER FAMILY OF CE SYSTEMS
Beckman Coulter’s history, knowledge and leadership
in Capillary Electrophoresis has lead to the creation
of the P/ACE and CEQ product lines. From its first
commercial instrument shipment in 1989, Beckman
Coulter’s leadership has established the largest
productive base of CE instruments and users in the
world. CE product development has been guided by
suggestions from this large customer base as well as
collaborative work with experts in the field. The
present CE family is the result of these efforts.
SUMMARY
The P/ACE Series are automated, programmable
capillary electrophoresis systems designed to perform
fast separations from complex samples. Separations
occur in a capillary which is housed in an interchangeable cartridge
with circulating liquid coolant for efficient temperature control.
This finely regulated temperature control allows the use of highionic-strength buffers and large-bore capillaries necessary for
analyzing difficult samples. With CE, methods are fast to develop,
easy to validate, and cost effective to run. However, specialized
system designs are required to optimize performance for different
applications. To address these needs, Beckman offers a family of
CE instruments developed to apply CE technology to a variety of
challenging applications.
Each system includes components necessary to begin
immediate operation, such as vials, capillaries, and test mix.
All systems include installation and basic training by qualified
Field Service Engineers.
B E C K M A N C O U LT E R C E S YS T E M S
P/ACE MDQ
QUALITY
CONTROL
SYSTEM
P/ACE MDQ
METHODS
DEVELOPMENT
SYSTEM
P/ACE MDQ
CHIRAL
A N A LY S I S
SYSTEM
P/ACE MDQ
G LY C O P R O T E I N
A N A LY S I S
SYSTEM
P/ACE
MDQ DNA
SYSTEM
CEQ
2000XL
DNA
SEQUENCER
TA B L E
OF
P/ACE
MDQ-LCQ
CE-MS/MS
P A R A G O N ®®
CZE 2000
S ERUM P R OTEIN
A NALYZER
NALYZER
CONTENTS
P/A C E M D Q S Y S T E M S . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
P /A C E M D Q
AND
CEQ SYSTEMS ...........................9
P/A C E M D Q M O D U L E S . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 0
D E T E C TO R O P T I O N S . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 1
P / A C E M D Q / 5 0 0 0 S E R I E S D E T E C TO R A C C E S S O R I E S . . . 1 2
C E S Y S T E M S O F T WA R E . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 4
CE SUPPLIES ................................................15
C E A P P L I C AT I O N K I T S . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 2
C E C H E M I S T RY K I T S . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 5
L A B WA R E . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 8
CE SUPPLIES
AND
L A B WA R E . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 0
C U S TO M E R S U P P O RT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2
C A P I L L A RY E L E C T R O P H O R E S I S E D U C AT I O N A L A I D S . . . . . . . 4 4
T E C H N I C A L & A P P L I C AT I O N I N F O R M AT I O N . . . . . . . . . . . . . . . . 4 6
INDEX ........................................................50
Beckman Coulter
Index
5
P/ACE MDQ
™
he P/ACE™ MDQ is an advanced CE system designed, in terms of flexibility and capacity, to meet the different requirements of research,
methods development and quality control laboratories. Each configuration is available with a series of options to customize to meet end-user
requirements.
All P/ACE MDQ configurations combine hardware, software and chemistry with worldwide technical support. A choice of interchangeable
detectors meets the needs of a wide range of applications. The software automates all operations, from direct instrument control to data acquisition,
analysis and reporting. At the heart of the MDQ is the system’s flexibility in programming and capacity. In methods development, samples can be
automatically run against an array of up to 36 different pairs of buffers, while in quality control the buffer array can be switched for buffer capacity
(large volume reservoir) enabling routine analysis of up to 96 samples. The P/ACE MDQ works with a range of sample vessels accommodating
96-well plates or a 48-position tray that holds 2 mL vials, 0.5 mL tubes and PCR* reaction vials. The system’s built in software features assist
in meeting GLP and CGMP compliance requirements. P/ACE MDQ is specially designed to meet industry’s demands for ruggedness and safety.
Beckman Coulter validates system performance upon installation and provides a comprehensive compliance protocol.
T
* PCR is covered by U.S. patents owned by Hoffmann-La Roche Inc.
P/ACE MDQ Methods Development System
PART N UMBER
PART N UMBER
149817 North America
International 285400
A CE-based analytical system configured optimally for the methods
development process, where a focus is placed on spectral analysis and the
automation of separation strategies. The system includes a P/ACE MDQ
configured with both a photo diode array and selectable-wavelength UV/Vis
(200, 214, 254 and 280 nm filters included) detector, UV source optics,
temperature-controlled sample storage module and 32 Karat™ Software
configured on an IBM personal computer. Installation Qualification,
Operation Qualification 1 (OQ1) and documentation to aid in software
validation is also included.
Starter chemistries: P/ACE MDQ Methods Development Kit, Amine
Capillary Validation Pack and the Neutral Capillary Validation Pack.
P/ACE MDQ Chiral System
PART N UMBER
PART N UMBER
149833 North America
International 285402
A CE-based analytical system configured optimally for developing methods
for the separation of enantiomers. The system includes a P/ACE MDQ
configured with a photo diode array detector, UV source optics, ambient
sample storage module and 32 Karat software configured on an IBM
personal computer. Installation Qualification, Operation Qualification 1
(OQ1) and documentation to aid in software validation is also included.
Starter chemistries: The Highly Sulfated Cyclodextrins chiral methods
development pack (strategy # 1), Neutral CD Chiral Methods Development
Kit (strategy #2), and Chiral Methods Development Guide.
P/ACE MDQ Quality Control System
PART N UMBER
PART N UMBER
149815 North America
International 285401
A CE-based analytical system configured optimally for the quality
assurance laboratory. With a focus on repetitive robust analyses, the
system includes a P/ACE MDQ configured with a filter-based selectablewavelength UV/Vis (200, 214, 254 and 280 nm included) detector,
UV source optics, ambient sample storage module, and 32 Karat Software
configured on an IBM personal computer. Installation Qualification,
Operation Qualification 1 (OQ1), and documentation to aid in software
validation are also included.
Beckman Coulter
Contents
Index
6
P/ACE MDQ
™
P/ACE™ MDQ-LCQ CE-MS/MS Systems
PART N UMBER
PART N UMBER
149016
These systems feature single-point control of both the ThermoFinnigan
LCQ and the Beckman Coulter P/ACE MDQ using the Xcalibur™
workstation. The P/ACE MDQ is configured with diode array detection,
sample tray temperature control, and capillary temperature control
extended to an electrospray interface. The unique system interface
employs a liquid sheath make-up flow to sweep the analytes off the
capillary and into the mass spectrometer. The LCQ utilizes ion trap
technology to resolve the molecular ions and is capable of MS/MS—
allowing these ions to be isolated and further fragmented to gain
structural information and higher confidence confirmation. These
systems have been optimized for capillary lengths and instrument
levels—and configured for the following three applications (please
contact your local Beckman Coulter representative for a quotation on
the following systems):
1) CE-MS/MS Protein Characterization System
2) CE-MS/MS Pharmaceutical Analysis System
3) CE-MS/MS Research System
P/ACE MDQ Glycoprotein System
PART N UMBER
PART N UMBER
149893 North America
International 149872
CE-based system for the separation and characterization of proteins
and associated carbohydrates. The system is ideal for peptide mapping,
separation of proteins by molecular weight, accurate isoelectric point
(pI) determination, oligosaccharide profiling and monosaccharide
analysis. System includes a P/ACE MDQ configured with a selectable
wavelength UV/Vis (200, 214, 254 and 280 nm included) detector,
UV source optics, laser-induced fluorescence detector, 488 nm argon
ion laser module, a temperature-controlled sample storage module,
and 32 Karat™ Software configured on an IBM personal computer.
Installation Qualification, Operation Qualification 1 (OQ1) and
documentation to aid in software validation is also included.
Starter chemistries: eCAP™ Carbohydrate Labeling and Analysis Kit,
eCAP SDS 14-200 Kit, MDQ Methods Development kit, LIF and UV
detector test mix, and Glycoprotein Methods Development Guide.
Beckman Coulter
Contents
Index
7
P/ACE MDQ
™
P/ACE™ MDQ Molecular Characterization System
PART N UMBER
PART N UMBER
149891 North America
International 149863
A CE-based system for the analysis of nucleic acids and their subsequent interactions with proteins. The system provides clear advantages
over traditional electrophoresis for DNA quantitation (viral load, gene
expression), synthetic oligonucleotide purity analysis, mutation analysis,
size polymorphisms analysis (VNTR, STR, microsatellites, RFLP,
RAPD) and the study of DNA/protein interactions. System includes
a P/ACE MDQ configured with a selectable-wavelength UV/Vis
(200, 214, 254 and 280 nm included) detector, UV source optics, dualwavelength laser-induced fluorescence detector, 488 nm argon ion laser
module, a temperature-controlled sample storage module, and 32 Karat™
Software configured on an IBM personal computer. Installation
Qualification, Operation Qualification 1 (OQ1) and documentation
to aid in software validation is also included.
Starter chemistries: P/ACE MDQ Methods Development Kit, eCAP™
ssDNA 100 kit, eCAP dsDNA 1000 kit, eCAP dsDNA 20,000 kit,
EnhanCE™ DNA intercalator, LIF and UV detector test mix, and
Molecular Characterization Applications Guide.
P/ACE™ MDQ Carbohydrate System
PART N UMBER
PART N UMBER
149890 North America
International 149896
2.0
Man Nac from Neu5Ac
A
0.1 N TFA hydrolyzed
Aldolase treated
GlcNAc
B
1.0
Man
GalNAc
Gal
2.0 N TFA hydrolyzed
Reacetylated
R
GalNAc GlcNAc
Man
Glc
Fuc
Gal
Standard
Six monosaccharides
2.00
4.00
Beckman Coulter
6.00
8.00
10.00
A CE-based system for the separation and analysis of carbohydrates. The
system is ideal for both oligosaccharide profiling and monosaccharide
analysis. System includes a P/ACE MDQ configured with a laser-induced
fluorescence detector, 488 nm argon ion laser module, an ambient sample
storage module, and 32 Karat™ Software configured on an IBM personal
computer. Installation Qualification, Operation Qualification 1 (OQ1)
and documentation to aid in software validation is also included.
Starter chemistries: eCAP™ Carbohydrate Labeling and Analysis Kit and
Carbohydrate Methods Development Guide.
Contents
Index
8
P/ACE MDQ AND CEQ SYSTEMS
™
™
P/ACE MDQ DNA System
PART N UMBER
PART N UMBER
149892 North America
International 149874
A CE-based system for the analysis of nucleic acids. The system provides
clear advantages over traditional electrophoresis for DNA quantitation
(viral load, gene expression), mutation analysis, and size polymorphisms
analysis (VNTR, STR, microsatellites, RFLP, RAPD). System includes
a P/ACE MDQ configured with a laser-induced fluorescence detector,
488 nm argon ion laser module, an ambient sample storage module, and
32 Karat Software configured on an IBM personal computer. Installation
Qualification, Operation Qualification 1 (OQ1) and documentation to aid
in software validation is also included.
Starter chemistries: eCAP dsDNA 1000 kit, eCAP dsDNA 20,000 kit,
EnhanCE DNA intercalator, LIF detector test mix, and Nucleic Acids
Methods Development Guide.
C E Q ™ 2 0 0 0 X L D N A A N A LY S I S S Y S T E M
The CEQ 2000XL DNA Analysis System is a fully automated, high-throughput sequencing and fragment analysis system. Capillary
electrophoresis technology and laser-induced fluorescence detection have been combined to provide a very easy to use microplatebased system. The CEQ is designed to handle all aspects of the DNA analysis process from sample denaturation through automatic
base or allele calling, with no user intervention, following an easy ten minute setup.
KEY FEATURES OF THE CEQ 2000XL
• Linear Polyacrylamide Gel matrix inside a coated capillary array provides the best read length/cycle time and the highest accuracy of any CE-based sequencer.
• Eight capillary array to eliminate plate cleaning, gel casting, and tedious DNA sample loading.
• 96-well microplate format.
• Automated: loading of separation gel, sample denaturation, and sample loading.
• Automatically switches between sequencing applications and fragment analysis on the same plate.
For Sequencing:
• Longer reads and faster cycle times (>700 bases in less than 100 minutes at better than 98% accuracy).
• Short reads and faster cycle times (>500 bases in less than 45 minutes at better than 98% accuracy).
• Dideoxy-terminator cycle sequencing kits with four “WellRED” fluorescence dyes (one-tube chemistry).
• Raw data and analyzed data available in most popular formats.
• Confidence values and call scores provided with each base determined.
For Fragment Analysis:
• Automated allele determination.
• Precisely sized microsatellite amplification products to a S.D. of ≤0.27 bases.
PART N UMBER
285501 Worldwide
The CEQ 2000XL DNA analysis system is a fully automated, highperformance sequencing and fragment analysis system, employing an
eight-capillary array to increase sample throughput, and specialized
laser-induced fluorescence detection optics with WellRED dyes
(infrared emission) for unsurpassed performance in dideoxy terminator
cycle sequencing. Sample introduction is done directly from a 96-well
microplate, compatible with most forms of sample prep automation.
The CEQ 2000XL handles all aspects of the DNA analysis process,
from sample denaturation through automatic base or allele calling with
no user intervention. This system includes the CEQ 2000XL instrument,
software for both sequencing and fragment analysis, an IBM
workstation, installation and basic training.
Beckman Coulter
Contents
Index
9
P/ACE MDQ MODULES
™
T
he following instruments are packaged with appropriate shipping kits to ensure rapid installation, but they do not include
computer workstations, software or accessories. See previous section for complete packages. The purpose of these systems
is to allow you to purchase basic modules to add on to existing systems.
PART N UMBER
D ESCRIPTION
144000
P/ACE™ MDQ – PDA
Includes P/ACE MDQ with photodiode array detector and ambient
sample storage module.
144001
P/ACE MDQ – UV Ambient
Includes P/ACE MDQ with UV/Vis detector and ambient sample
storage module.
144002
P/ACE MDQ – PDA – Sample Temp. Control
Includes P/ACE MDQ with Diode Array Detector and temperaturecontrolled sample storage module.
144003
P/ACE MDQ – UV – Sample Temp. Control
Includes P/ACE MDQ with UV/Vis detector and temperature-controlled
sample storage module.
Beckman Coulter
Contents
Index
10
DETECTOR OPTIONS
P/A C E™ M D Q D E T E C T O R S
Beckman Coulter believes that an analytical instrument should be able to evolve to meet changing requirements.
The following detector modules are plug-in units that are easy to switch once they are installed.
PART N UMBER
D ESCRIPTION
144733
P/ACE™ MDQ UV/Vis Detector Module
Consists of UV/Vis detector, UV optics cable, 200 nm, 214 nm, 254 nm,
and 280 nm filters.
149819
P/ACE MDQ Diode Array Detector Upgrade Kit
Consists of DAD detector, DAD optics cable, and DAD board.
149852
P/ACE MDQ External Detector Adapter (EDA)
Accessory Kit
Complete kit for the addition of EDA to MDQs shipped with
version 1.5 software and higher. These MDQs will already have
EDA-compatible hardware and firmware. Kit also includes a hydraulic
leveling table. Service installation included.
144954
P/ACE MDQ Dual λ LIF Detector
Includes LIF Dual Detector Module, Interconnect Module, LIF
Cartridge Plug and Stabilizer. Allows two emission wavelengths to be
detected simultaneously. For further information, see DS-8284A.
144901
P/ACE MDQ Single λ LIF Detector
Includes LIF Detector Module, Interconnect Module, LIF Cartridge
Plug and Stabilizer.
Laser Modules
Beckman Coulter
727620
Argon Ion – 488 nm (120 V)
727621
Argon Ion – 488 nm (220 V)
727622
Argon Ion – 488 nm (240 V)
727619
Argon Ion – 488 nm (100 V)
267839
635 nm Diode Laser
Contents
Index
11
P/ACE MDQ/5000 SERIES DETECTOR ACCESSORIES
™
PART N UMBER
D ESCRIPTION
LIF Detector Filters for P/ACE™ 5000 Series
360672
961640
960323
890880
890879
725412
LIF Detector Filter Holder
LIF 488 nm Notch Filter
LIF 520 nm Emission Bandpass Filter
LIF 530 nm Emission Bandpass Filter
LIF 560 nm Emission Bandpass Filter
LIF 675 nm Emission Bandpass Filter
UV Filters
Filters can be purchased for replacement or custom applications and
are customer installed. These filters are compatible with both the
P/ACE 5000 and the P/ACE MDQ instruments.
144430
144431
144437
144432
144433
144438
144434
144439
144435
144436
Filter, 200 nm
Filter, 210 nm
Filter, 214 nm
Filter, 220 nm
Filter, 230 nm
Filter, 254 nm
Filter, 260 nm
Filter, 280 nm
Filter, 300 nm
Filter, 340 nm
LIF Detector Filters for P/ACE MDQ Series
144941
144940
144942
Beckman Coulter
Contents
488 nm Notch Filter
520 nm Bandpass Filter
663 nm Longpass Filter
Index
12
P/ACE MDQ/5000 SERIES DETECTOR ACCESSORIES
™
Miscellaneous Supplies
PART N UMBER
D ESCRIPTION
360671
LIF Fiber-Optic Cable for Laser with
SMA905 Connector (quantity 1)
726022
LIF Test Mix, Sodium Fluorescein (20 mL)
477615
635 LIF Calibration/Test Standard
144667
Deuterium Lamp Assembly, P/ACE™ MDQ
Replacement part (quantity 1).
142205
Deuterium Lamp Assembly, P/ACE 5000 Series
Replacement part (quantity 1).
359058
Deuterium Lamp Assembly, P/ACE 2000 Series
Replacement part (quantity 1).
Beckman Coulter
144093
Replacement UV/Vis Fiber Optic Cable for the
P/ACE MDQ
144094
Replacement DAD Fiber Optic Cable for the
P/ACE MDQ
Contents
Index
13
CE SYSTEM SOFTWARE
3 2 K A R AT ™ S O F T WA R E
AND
WO R K S TAT I O N S
32 Karat™ software is a new platform for P/ACE™ MDQ system control, data collection, and analysis. This new platform was
developed entirely in 32-bit code to fully utilize the functionality of the Windows* NT and Windows 2000 operating systems.
32 Karat software preserves data integrity with features such as user-specific log-in, independent audit trails, and results sign-off—
ideal for research, biotechnology, or pharmaceutical laboratories. 32 Karat software adds analysis dimensions that significantly
increase the accuracy of compound identification. New Peak identification algorithms allow the P/ACE MDQ to combine an
analyte’s mobility with spectral information for accurate identification against a large database of compounds. Data may also be
plotted as a function of either migration time or mobility.
Each 32 Karat workstation is capable of running up to four P/ACE MDQ systems with multiple channels. 32 Karat software
has been developed using documented processes and is fully validated at Beckman Coulter. The 32 Karat Workstation includes a
computer platform that has been validated by Beckman Coulter for the operation of 32 Karat software.
PART N UMBER
D ESCRIPTION
285510
32 Karat Workstation for P/ACE MDQ
Includes 32 Karat software installed on an IBM computer with Windows NT
software. Six hours of user training are also included. Contact Beckman
Coulter sales for the current computer configuration.
285512
32 Karat Software (User Installed)
Includes 32 Karat software and six hours of user training. Requires a
compatible computer platform (please contact your local Beckman Coulter
representative).
285511
32 Karat Workstation – Migration Upgrade
Includes 32 Karat software installed on an IBM computer with Windows NT
software. A migration guide to assist you in moving your data and methods
from the P/ACE MDQ software to 32 Karat software and 6 hours of user
training are included. This migration offer requires you to return your existing P/ACE MDQ software key to Beckman Coulter (please contact your
local Beckman Coulter representative).
285514
32 Karat Software – Migration Upgrade
(User Installed)
Includes 32 Karat software and six hours of user training. Requires a
compatible computer platform (please contact your local Beckman
Coulter representative). A migration guide to assist you in moving your
data and methods from the P/ACE MDQ software to 32 Karat software
and 6 hours of user training are included. This migration offer requires you
to return your existing P/ACE MDQ software key to Beckman Coulter.
149948
32 Karat Software Reprocessing Key
Allows reprocessing of data collected on 32 Karat software at a
remote or network location (does not provide instrument control).
149989
32 Karat Software Validation Pack
System requirements, specifications, intended use, development life
cycle quality programs, and validation process are documented, allowing this information to be used as a “remote audit” to support the validation of Beckman Coulter COTS (Commercial Off-The-Shelf) analytical
software. Site audits at Beckman Coulter in Fullerton are also available.
PC Accessory Pak
Includes keyboard (local language version), monitor and PC power cords.
144930
144931
144932
144933
144934
Beckman Coulter
Contents
French Version
German Version
Italian Version
Spanish Version
U.K. Version
Index
14
CE SUPPLIES
CAPILLARIES
All methods development in CE begins with the choice of the correct
capillary surface. The goal is to choose a surface which will not interact
with the sample and, depending upon the application, one which will
either reduce or induce electroosmotic flow. Beckman Coulter offers a
choice of several types of capillaries, which range in surface chemistry
allowing a variety of samples to be analyzed including pharmaceuticals,
industrial chemicals, chiral drugs, nucleic acids, amino acids, proteins
and peptides. These capillaries are available in defined lengths, which
may be trimmed to optimize instrument type, speed and resolution.
NEUTRAL SURFACES
Neutral surfaces are designed to coat the exposed silanol groups on the fused silica and serve two major functions: 1) to reduce
electroosmotic flow, and 2) to prevent wall interaction with the sample. However, not all neutral surfaces are the same and it is
important to understand what the surface is composed of to ensure that other interactions (i.e., hydrophobic) do not replace the
ionic interactions you are trying to reduce. For example, when using a neutral capillary with a surface consisting of a hydrophobic
bonded phase, it is essential to include hydrophilic polymers in the buffer to prevent hydrophobic interactions. The following is
a list of neutral surfaces that are available from Beckman Coulter. All of these surfaces are bi-layers, consisting first of a bonded
phase to shield the silanol groups followed by a hydrophilic polymer to prevent hydrophobic interaction.
PART NUMBER
DESCRIPTION
eCAP™ Neutral Capillary, 50 µm I.D.,
45 cm total length, 12 cm from window to outlet
(quantity 1)
The neutral capillary utilizes a secondary layer of polyacrylamide
to generate a hydrophilic surface. This is a general purpose capillary,
which Beckman Coulter recommends as a starting point for handling
complex samples like proteins or basic molecules. Polyacrylamide
surfaces are recommended for use with both UV and LIF detection
systems but may show some reduction in capillary life with diodearray detection systems.
477441
eCAP N-CHO Capillary, 50 µm I.D.,
65 cm total length, 12 cm from window to outlet
(quantity 1)
The N-CHO capillary utilizes a secondary layer composed of polyvinyl
alcohol (PVA). This surface is ideal for the separation of complex samples like proteins or basic pharmaceuticals in cases where minimal EOF
is desired. PVA is a very rugged surface, which is stable with UV, LIF
and diode array detection systems. However, due to the chemical nature
of PVA, it is not recommended for use with borate buffer systems, as
the borate ion will complex with the surface. This capillary has been
featured in the Carbohydrate Labeling and Analysis Kit (P/N 477600).
477601
Beckman Coulter
Contents
Index
15
CE SUPPLIES
NEUTRAL SURFACES (CONTINUED)
PART NUMBER
DESCRIPTION
241521
eCAP™ Neutral Capillary (SDS-MW),
100 µm I.D., 65 cm total length,
12 cm from window to outlet (quantity 1)
This capillary has been optimized for use with the eCAP SDS 14-200
kit (separation of proteins by molecular weight). The capillary utilizes
a 100 µm I.D. which allows increased sample loads, ideal for isolation
of an analyte by fraction collection.
eCAP Neutral Capillary (DNA), 100 µm I.D.,
65 cm total length, 12 cm from window to outlet
(quantity 1)
This capillary has been optimized for use with Beckman Coulter
DNA chemistries. The capillary utilizes a 100 µm I.D. which allows
increased sample loads, ideal for isolation of an analyte by fraction
collection.
477477
1st and 240th runs of basic proteins on a neutral
capillary. %RSD for migration time: Lysozyme—0.47%,
Cytochrome C—0.51%, and Ribonuclease A—0.96%.
Beckman Coulter
Contents
Index
16
CE SUPPLIES
AMINE SURFACES
Although basic compounds can be analyzed effectively at low pH, there are circumstances where one may wish to operate at a
higher pH to better differentiate sample mobility, effect solubility or induce electroosmotic flow. The amine capillary was generated
for this purpose. The Beckman Coulter amine capillary utilizes a dynamic coating that, by washing with the amine regenerator
between runs, assures a uniform capillary coating from run to run. This capillary has been used effectively for basic proteins, basic
drugs and even for the separation of glycoforms. The surface is a cross-linked polymer with a quaternary ammonium ion providing
a uniform positively charged surface and a consistent electroosmotic flow.
PART N UMBER
3
45 7
2
D ESCRIPTION
477431
eCAP™ Amine Capillary, 50 µm I.D.,
65 cm total length, 12 cm from window to outlet
(quantity 1)
This capillary has been coated with the amine polymer and must be
used in combination with the amine regenerator solution P/N 477433.
6 8
1
477433
34
2
eCAP™ Amine Capillary, 75 µm I.D.,
65 cm total length, 12 cm from window to outlet
(quantity 1)
This capillary has been coated with the amine polymer and must be
used in combination with the amine regenerator solution P/N 477433.
5 7
8
6
1
The eCAP Amine capillary improves resolution of basic
drugs. (1)-Impurity, (2)-Doxepin, (3)-Nordoxepin,
(4)-Imipramine, (5)-Desipramine, (6)-Amitriptyline,
(7)-Nortriptyline, (8)-Protriptyline.
Beckman Coulter
Contents
Index
17
CE SUPPLIES
U N T R E AT E D F U S E D - S I L I C A C A P I L L A R I E S
Beckman Coulter bare fused-silica capillaries are routinely used for a variety of CE separations. The pKa of fused silica is
about 1.5. The degree of ionization of the walls of these capillaries is controlled by the pH of the buffers used for separation.
FUSED-SILICA CAPILLARIES
Pre-Burned Windows
20 µm I.D. ×
375 µm O.D.
338475
20 cm effective length
quantity 3
50 µm I.D. ×
375 µm O.D.
75 µm I.D. ×
375 µm O.D.
338451
50 cm effective length
quantity 3
338454
50 cm effective length
quantity 3
360801
111 cm total length to
interface to EDA
20 cm to window
quantity 3
360800
111 cm total length to
interface to EDA
20 cm to window
quantity 3
100 µm I.D. ×
375 µm O.D.
149053
115 cm total length; 100 cm
to window. Methods development capillary for CEMS/MS system.
Without Pre-Burned Windows
360804
5 meters total length
338472
5 meters total length
338473
5 meters total length
338474
5 meters total length
C E Q ™ 2 0 0 0 X L C A P I L L A R Y A R R AY S
Each array has eight coated capillaries, complete with inlet electrode cannula and detector array fitting. Ready for easy installation
into the CEQ™ 2000XL. Each capillary will last at least 100 runs depending on method used, enabling at least 800 separations to be
performed before replacement. Storage temperature: 6°C.
Beckman Coulter
PART N UMBER
D ESCRIPTION
608015
CEQ DNA Separation Array, 50–100 µm
50 cm in length, 100 µm I.D., 200 µm O.D.
608087
CEQ DNA Separation Array, 33–75 µm
33 cm in length, 75 µm I.D., 200 µm O.D.
Contents
Index
18
CE SUPPLIES
P/ACE™ MDQ CARTRIDGES
PART N UMBER
AND
ACCESSORIES
D ESCRIPTION
144738
Blank Cartridge Assembly Kit
Contains cartridge body, 100 × 800 µm aperture, tubing kit, nuts,
ferrules, and O-rings. Compatible with aqueous buffer systems.
149044
Blank Cartridge – CE-MS/CEC Kit
For use with CE-MS/MS or CEC applications where organic solvents
are primarily used. Kit contains cartridge body, 100 × 800 µm aperture,
tubing kit, nuts, ferrules, and O-rings.
144645
Cartridge Rebuilding Kit
Contains capillary length template, cleaving stone, rebuild instructions,
dual seals, single seals, clips, O-rings, installation tool, and tweezers.
970297
Cartridge Aperture O-Rings (quantity 2)
144689
Cartridge Tubing Kit
Consists of 20-, 30-, 40-, and 50-cm lengths of heat-formed tubing
and all connectors.
144717
Cartridge Tube Kit 100
Cartridge coolant tubing (100 cm total length).
144866
Cartridge Clip with Dual Seal
(quantity 4 red seals)
Capillary cartridge clip and dual seal is designed to seal the capillary
junction between the bottom of the P/ACE MDQ cartridge and the
interface block. Seal can be used for capillaries with outside diameters
as small as 150 µm.
144873
Cartridge Clip with Single Seal
(quantity 4 green seals)
Cartridge clip with single seal is designed to seal the capillary junction
around the optics window in the P/ACE MDQ cartridge.
144711
Aperture, 100 × 800 µm (kit of 3)
Recommend replacement after 3 months of use.
144712
Aperture, 100 × 200 µm (kit of 3)
Recommend replacement after 3 months of use.
144829
EDA Cartridge and Adapter Kit
New external detector adapter cartridge with all tubing, clips, seals, etc.,
and the adapter couplings. Cartridge is detector bypass type.
144822
EDA Capillary Adapter Assembly
External detector adapter body, adapter nuts, seals, fittings, and tubing
kit. Used to make existing MDQ cartridges into EDA (utilizes the
window). No cartridge included.
144834
EDA Tubing Kit
Consists of precut EDA tubing, nuts, O-rings, and ferrules.
Beckman Coulter
Contents
Index
19
CE SUPPLIES
P/ACE™ 5000 CARTRIDGES & ACCESSORIES
eCAP™ Cartridges With Capillaries
for P/ACE 5000 Series
Each capillary cartridge below contains a fused-silica capillary and is
designed with a prealigned aperture for on-capillary detection. Circulation
ports permit flow of inert heat transfer fluid to ensure efficient temperature
control. Capillary lengths listed are effective lengths from inlet to detector.
PART N UMBER
D ESCRIPTION
727604
Capillary Cartridge
Includes tested fused-silica capillary, 50 cm length, 50 µm I.D.,
375 µm O.D., 100 × 200 µm aperture. Capillaries pretreated with
0.1 N NaOH; run with Test Mix, P/N 338425, and Run Buffer A,
P/N 338426. The test mix electropherogram is included.
727605
Capillary Cartridge
Includes tested fused-silica capillary, 50 cm length, 75 µm I.D.,
375 µm O.D., 100 × 200 µm aperture. Capillaries pretreated with
0.1 N NaOH; run with Test Mix, P/N 338425, and Run Buffer A,
P/N 338426. The test mix electropherogram is included.
727607
High-Sensitivity UV Cartridge
Includes tested fused-silica capillary, 50 cm length, 50 µm I.D.,
375 µm O.D., 100 × 800 µm aperture. Capillaries pretreated with
0.1 N NaOH; run with Test Mix, P/N 338425, and Run Buffer A,
P/N 338426. The test mix electropherogram is included.
727606
High-Sensitivity UV Cartridge
Includes tested fused-silica capillary, 50 cm length, 75 µm I.D.,
375 µm O.D., 100 × 800 µm aperture. Capillaries pretreated with
0.1 N NaOH; run with Test Mix, P/N 338425, and Run Buffer A,
P/N 338426. The test mix electropherogram is included.
360604
LIF Cartridge
Includes tested fused-silica capillary, 50 cm in length, 75 µm I.D.,
375 µm O.D.
The Capillary Replacement Kit, P/N 338432, and capillary tubing should also
be purchased in order to replace the capillary. The eCAP Capillary Cartridge
can accommodate capillary tubing from 20 cm to 120 cm in length.
Beckman Coulter
Contents
Index
20
CE SUPPLIES
P/ACE™ 5000 CARTRIDGES & ACCESSORIES
eCAP™ Blank Capillary Cartridges
for P/ACE 5000 Series
Insert a capillary into this cartridge to run on the P/ACE 5000 series.
PART N UMBER
D ESCRIPTION
727610
Blank High Sensitivity UV Cartridges
100 × 800 µm aperture
727608
Blank Capillary Cartridge
100 × 200 µm aperture
338449
Blank Capillary Cartridge
50 × 200 µm aperture
360692
LIF Cartridge Blank
338432
eCAP Capillary Replacement Kit
Provides materials required to build your own cartridge
(requires capillary tubing and blank capillary cartridge) or
rebuild your existing capillary cartridge (requires capillary tubing).
Includes necessary tools, detailed instructions, and materials for
building/rebuilding 25 cartridges. Tools and materials include:
capillary alignment plate, cleaving stone, clamps, gaskets, sealant
strips, plugs, tools, and capillary retaining tubing.
338431
Consumable Kit
Replacement supplies for the Capillary Replacement Kit. This kit
includes gaskets, sealant strips, plugs, and capillary retainer tubing as
required for building/rebuilding 25 cartridges.
Beckman Coulter
Contents
Index
21
CE APPLICATION KITS
s the chemistry is an important part of any application, Beckman Coulter has developed a wide variety of application kits.
These kits increase productivity by speeding up the methods development process and ensuring consistent reagent performance. Each kit contains the capillaries, buffers, standards and instructions necessary for the analysis. The standard test
protocols allow for rapid suitability testing of the capillary prior to the analysis of the chosen analyte.
A
METHODS DEVELOPMENT
The P/ACE™ MDQ Methods Development Kit is designed to give a starting point for the development of a capillary zone electrophoresis analysis of complex proteins, pharmaceuticals or basic analytes. This kit includes both neutral (polyacrylamide-based)
and amine coated capillaries. Benzyl alcohol is included as a neutral EOF marker while histamine is used as a reference marker for
the neutral capillary.
PART N UMBER
D ESCRIPTION
501310
P/ACE MDQ Methods Development Kit
Consists of an amine capillary, a neutral capillary, 20 mM citrate/
MES buffer, pH 6.0, 50 mM Tris buffer, pH 8.0, Small Molecule
Test Mix, Protein Test Mix, histamine reference marker, neutral marker,
amine regenerator solution, and care and use guide.
Reorder Components
477441
477431
477433
477443
477427
Separation of Small Molecule Test Mix on a 50 cm
Amine Capillary (50 µm I.D.) using 50 mM Tris
Buffer, pH 8.0, at 25 kV, UV 254 nm, 3 second 0.5 psi
pressure injection.
Beckman Coulter
eCAP™ Neutral Capillary
50 µm I.D., 45 cm total length (quantity 1)
eCAP Amine Capillary
50 µm I.D., 65 cm total length (quantity 1)
Amine Regenerator Solution
Citrate/MES Buffer, pH 6.0
Tris Buffer, pH 8.0
477421
Small Molecule Test Mix (4.0 mL)
Includes:
N-acetylprocainamide, 250 µg/mL
Procainamide, 350 µg/mL
Nicotine
477436
Protein Test Mix
Includes:
Lysozyme, 1 mg
Cytochrome C, 1 mg
Ribonuclease A, 1 mg
477434
477446
Neutral Marker
Histamine Reference Marker
Contents
Index
22
CE APPLICATION KITS
C H I R A L A N A LY S I S
Since the chemical properties of enantiomers are identical, typical measurements and separation techniques cannot be used
to distinguish one from the other. The key to separating enantiomers is to first create diastereomers from the enantiomers.
Diastereomers may be created through chemical derivatization with a “chiral” reagent, or they may be formed transiently through
interactions with chiral selectors. The latter is usually the most desirable as it is the easiest to employ.
Capillary electrophoresis has proven the most ideal analytical tool for this purpose, as it is simple to construct and modify a chiral
environment within a capillary. The use of cyclodextrins for differential host-guest complexation of enantiomers is by far the most
common chiral selector and is the basis of the chiral separation strategy that we propose. Beckman Coulter’s primary strategy
focuses on the use of highly sulfated cyclodextrins (HSCDs) which are a family of three chiral reagents. This strategy first involves
screening the compound for separation using all three (α, β, and γ) HSCDs and then optimizing on the reagent which yields the
best resolution. This initial screen involves the use of a single method and has been successful in greater than 90% of the
compounds that Beckman Coulter has analyzed.
PRIMARY STRATEGY
Primary Screen
• 50 µm bare fused silica Capillary
– 20 cm to Detector
•
•
•
•
•
Polarity – anode at detector
Detection – 200 nm
Buffer – 50 nM phosphate pH. 2.5
Selector – α, β and γ HSCD
15 kV constant voltage
YES
Use CD
which gave
best
resolution and
vary
concentration
Further
optimize
temperature
column
length, field
strength
Did you
achieve
Resolution?
NO
Did peak
shape
broaden
YES
NO
Increase
HSCD to
10% and try
all 3 HSCD’s
Decrease
[HSCD}
to 2%
Resolution?
YES
NO
Optimize
Fine-tune HSCD
concentration
Temperature
Resolution?
NO
YES
Go to
Secondary
Strategy
Go to
Secondary
Strategy
Capillary Length
Field Strength
Beckman Coulter
Contents
Index
23
CE APPLICATION KITS
C H I R A L A N A LY S I S ( C O N T I N U E D )
Highly Sulfated Cyclodextrins
The HSCDs are a proprietary distribution of cyclodextrins with an average number of 12 sulfates for the β-CD, 11 for the α-CD and 13 for the
γ-CD. These distributions were preferentially selected over single isomers
to provide increased resolution under defined separation conditions.
These products perform well for the analysis of many neutral, basic and
weakly acidic compounds of pharmaceutical and biological interest.
To ensure batch-to-batch consistency, Beckman Coulter has developed a
process which generates the final product as a 20% w/v solution of the
HSCDs. Our processes in combination with a very rigorous quality control program ensure consistent matching of all lots to a Gold standard.
PART N UMBER
D ESCRIPTION
149869
Highly Sulfated Cyclodextrin 20% w/v Trial Kit
Consists of HS α Cyclodextrin (5 mL) 20% w/v, HS β Cyclodextrin
(5 mL) 20% w/v, HS γ Cyclodextrin (5 mL) Test Mix, PTS Marker
(1 mL @ 10 mMolar), Capillary Conditioning Solution and Phosphate
Buffer, pH 2.5 (50 mL).
713348
713349
713331
713347
713350
713351
HS α Cyclodextrin (5 mL) 20% w/v
HS α Cyclodextrin (100 mL) 20% w/v
HS β Cyclodextrin (5 mL) 20% w/v
HS β Cyclodextrin (100 mL) 20% w/v
HS γ Cyclodextrin (5 mL) 20% w/v
HS γ Cyclodextrin (100 mL) 20% w/v
713330
HS Cyclodextrin Test Mix
Includes:
• pseudoephedrine enantiomers
• glutethimide enantiomers
713328
713333
477422
PTS Marker (1 mL @ 10 mMolar)
Capillary Conditioning Solution (10 mL)
Phosphate Buffer, pH 2.5 (50 mL)
Clicking on the picture at the left will open
A-1889A, "Methods Development Strategy
for Enantiomer Analysis Using the P/ACE
MDQ Chiral System"
Example of amphetamine enantiomers separated with highly
sulfated γ cyclodextrin.
Beckman Coulter
Contents
Index
24
CE APPLICATION KITS
C H I R A L A N A LY S I S ( C O N T I N U E D )
I
f no resolution is obtained from the primary strategy, Beckman Coulter offers a secondary approach with the P/ACE MDQ
Chiral Methods Development Kit. This kit utilizes a selection of four non-charged cyclodextrins.
CHIRAL METHODS DEVELOPMENT SECONDARY STRATEGY
Choose UV wavelength
Choose CD type
or try all 4 CDs
Separate with:
High pH Buffer
Low and High [CD]
Reversed Polarity
Chiral Separation?
ACID
N
BASE
Solute: Acid or Base
N
Determine pK of solute
Y
Separate with:
Low pH Buffer
Low and High [CD]
Normal Polarity
Chiral Separation?
Y
Separate with:
pH = pK Buffer
Low and High [CS]
Acids: Reversed Polarity
Bases: Normal Polarity
N
Chiral Separation?
Try other Chiral Selectors
Y
Optimize
Determine
pK of solute
[CD]; E; T; pH
PART N UMBER
D ESCRIPTION
501300
P/ACE MDQ Chiral Methods Development Kit
Consists of 2 neutral capillaries, high pH buffer, low pH buffer,
adjustable pH buffer, β-cyclodextrin, γ-cyclodextrin, HP-β-cyclodextrin,
and DM-β-cyclodextrin, Test Mix, Methods Development Guide, Chiral
Methods Development Flow Chart, and Buffers Dilution Guide.
Beckman Coulter
Contents
Index
25
CE APPLICATION KITS
C H I R A L A N A LY S I S ( C O N T I N U E D )
Reorder Components
PART N UMBER
D ESCRIPTION
477441
eCAP™ Neutral Capillary
50 µm I.D., 45 cm total length
Chiral Kit High pH Buffer (500 mL)
Chiral Kit Low pH Buffer (500 mL)
Chiral Kit Adjustable pH Buffer (500 mL)
Chiral Kit Test Mix (1 mL)
β-cyclodextrin (25 g)
γ-cyclodextrin (25 g)
Dimethyl-β-cyclodextrin (25 g)
Hydroxypropyl-β-cyclodextrin (25 g)
477449
477448
477447
477469
477437
477438
477467
477470
A typical separation of the Chiral Kit Test Mix
(DANS-DL-LEU), using the Chiral Kit High pH
Buffer.
Beckman Coulter
Contents
Index
26
CE APPLICATION KITS
P R O T E I N A N A LY S I S
Proteins make up one of the most analytically challenging group of compounds due to their complexity and charge heterogeneity. Yet
CE has been an ideal analytical tool for this class of compounds. As there is no one single methodology that can cover all proteins,
Beckman Coulter has put together a series of methods development kits to help you design CE methodology for protein analysis.
These kits are developed as “getting started” chemistries and include 1) an isoelectric focusing kit which offers a general screen of a
broad range of isoelectric points, 2) a kit to allow the separation of proteins by size using methodology analogous to SDS-PAGE,
and 3) a capillary zone electrophoresis methods development kit, utilizing a neutral capillary coating to minimize protein interaction
with the wall.
PART N UMBER
D ESCRIPTION
477490
eCAP™ cIEF 3-10 Screening Kit
Isoelectric focusing (IEF) is commonly used in both research and
industrial settings for purity assessment, identity, monitoring of
degradation products, and microheterogeneity analysis. The eCAP cIEF
3-10 Kit provides an advanced alternative to slab gel isoelectric
focusing. With the automated features of CE, loading, focusing,
mobilizing and detecting proteins is simple. The ampholyte in this kit
covers a wide range from pH 3.0 to 10.0. This will provide a good
starting range to allow for screening for the pI in a single run.
Resolution of proteins with as little as 0.05 pI unit differences are
possible using the eCAP Neutral Capillary which minimizes EOF and
provides sharp, focused zones.
The eCAP cIEF 3-10 Screening Kit contains (2) eCAP Neutral
coated capillaries, ampholytes (3-10), cIEF polymer solution, anolyte,
catholyte, pI standards and instructions for system set up and analysis.
32 Karat™ software allows the construction of isoelectric point plots
such that the pIs of unknowns can be calculated automatically.
Reorder Components
477441
Protein standards: Ribonuclease A (pI 9.45),
Carbonic Anhydrase II (pI 5.9), β-Lactoglobulin
A (pI 5.1), CCK Flanking Peptide (pI 2.75).
477488
477497
477487
eCAP Neutral Capillary, 50 µm I.D.,
45 cm total length, 14 cm from window to outlet
(quantity 1)
cIEF Ampholyte, 3-10
(quantity 1 mL)
cIEF Polymer Solution
(quantity 100 mL)
cIEF Protein Standards
Includes:
Ribonuclease A (pI 9.45)
(quantity 3.84 mg)
Carbonic Anhydrase II (pI 5.9)
(quantity 0.32 mg)
β-Lactoglobulin A (pI 5.1)
(quantity 0.64 mg)
CCK Flanking Peptide (pI 2.75)
(quantity 0.16 mg)
A calibration curve from the standards included in
the cIEF 3-10 kit. Constructed by plotting the pIs
of these proteins against their migration time.
Beckman Coulter
Contents
Index
27
CE APPLICATION KITS
P R O T E I N A N A LY S I S ( C O N T I N U E D )
PART N UMBER
D ESCRIPTION
477420
eCAP™ SDS 14-200 Kit
One of the most common methods used to assess the molecular
weights of proteins has been SDS-PAGE. Beckman Coulter has
automated SDS molecular weight and purity determinations with the
development of the eCAP SDS 14-200 Kit. With CE, proteins are
detected directly using UV detection at 214 nm, resulting in accurate
quantitation without the need for staining. Results are obtained in only
15 minutes, with no gel preparation. Samples are treated in the same
manner as SDS-PAGE: heat denaturation in the presence of SDS and
optional disulfide bond reduction with β-mercaptoethanol. The
separation is by capillary gel electrophoresis using a formulation of
polyethylene oxides optimized for the resolution of proteins from
14,000 to 200,000 daltons. A polyacrylamide coated neutral capillary
is used to eliminate EOF and minimize sample interaction with the wall.
This results in very reproducible separations with run-to-run, day-to-day
migration time RSDs less than 1%. The capillary coating is robust,
allowing acid washes between each run. The gel (buffer) is replaceable,
allowing a “fresh start” with each sample. With glycoproteins,
discrepancies in molecular weight can be minimized with the use of the
Ferguson method. The gel buffer can be easily diluted and placed in the
autosampler at several different concentrations, allowing the Ferguson
analysis to be automated.
Each kit contains a neutral capillary, buffers, test mix and
markers to perform at least 200 runs.
Reorder Components
241521
Separations of Interleukens 3 and 6 by SDS-MW
analysis using eCAP SDS 14-200 Kit.
477416
477418
241524
241525
Beckman Coulter
Contents
eCAP Neutral Capillary (SDS), 100 µm I.D.,
65 cm total length, 12 cm from window to outlet
(quantity 1)
SDS 14-200 Gel Buffer (quantity 55 mL)
Protein Test Mix (quantity 3.4 mg total protein)
α-Lactalbumin (14.2 kD)
Carbonic Anhydrase (29 kD)
Ovalbumin (45 kD)
Bovine Serum Albumin (66 kD)
Phosphorylase B (97.4 kD)
β-Galactosidase (116 kD)
Myosin (205 kD)
Orange G Reference Marker (0.1% solution)
(quantity 1 mL)
Sample Buffer (0.12 M Tris-HCl, 1% SDS, pH 6.6)
(quantity 50 mL)
Index
28
CE APPLICATION KITS
P R O T E I N A N A LY S I S ( C O N T I N U E D )
PART N UMBER
D ESCRIPTION
477445
eCAP™ Neutral Capillary Methods Development
Kit for Proteins
The heterogeneous nature of proteins makes it almost impossible to
design a single method that will work for all. With capillary zone
electrophoresis, analytes are resolved due to differences in expressed
charge relative to mass. A protein’s pI, its solubility state, presence of
prosthetic groups or extent of glycosylation will all impact the choice
of the appropriate buffer for the CZE analysis. Moreover, at neutral to
basic pH, the surface of the capillary is highly charged and can act as
an adsorptive surface for many proteins. The eCAP Neutral Capillary
Methods Development Kit utilizes a capillary with a neutral
polyacrylamide-bonded surface, rendering it nonreactive to most
proteins. The kit also contains a series of buffers, test compounds, and
markers to assist you in developing a method for the analysis of your
protein.
Reorder Components
477441
477442
477443
477444
477446
241524
477436
eCAP Neutral Capillary, 50 µm I.D.,
45 cm total length, 14 cm from window to outlet
(quantity 1)
50 mM Citrate Buffer, pH 3.0 (quantity 100 mL)
50 mM Citrate/MES Buffer, pH 6.0
(quantity 100 mL)
50 mM Tricine Buffer, pH 8.0 (quantity 100 mL)
Histamine Reference Marker (cation)
(quantity 1 mL)
Orange G Reference Marker (anion)
(quantity 1 mL)
Protein Test Mix
1st and 120th run of acidic proteins
using the eCAP Neutral Capillary
Methods Development Kit.
Beckman Coulter
Contents
Index
29
CE APPLICATION KITS
C A R B O H Y D R AT E A N A LY S I S
Carbohydrate analysis is essential to fully characterize a glycoprotein-based biopharmaceutical and is considered to be a challenging
and formidable task. At first glance, CE does not appear to be ideal in that most carbohydrates lack readily ionizable functional
groups and the ability to either absorb or fluoresce. This situation is easily remedied with a simple reductive amination reaction
using 1-aminopyrene-3,6,8-trisulfonate (APTS). This reaction provides specificity, ample charge for mobility and strong fluorescence for detection with laser-induced fluorescence (LIF). APTS has been used successfully for the derivatization of both oligosaccharides and monosaccharides and coupled with CE-LIF provides more quantitative information with increased resolving power.
PART N UMBER
D ESCRIPTION
477600
eCAP™ Carbohydrate Labeling and Analysis Kit
This chemistry includes the components necessary to label
and separate oligosaccharides. The kit includes:
NCHO coated capillary
NCHO separation buffer
Labeling reagents:
- Quantitation control (maltose)
- Labeling dye (APTS)
- Labeling dye solvent (15% acetic acid)
- Glucose ladder (standard)
- APTS-M (monosaccharide-grade)
-O3S
NH2
501309
CE-Grade APTS Carbohydrate Labeling Reagent
APTS is synthesized at Beckman Coulter as a highly purified
reagent ideal for labeling the reducing terminus of
carbohydrates. Its (-) 3 charge provides high mobility for
very rapid analysis of carbohydrates by capillary zone
electrophoresis. This reagent is suitable for labeling
oligosaccharides.
APTS Formula:
Labeling:
Excitation λ:
Emission λ:
Formula Wt:
Qty:
-O3S
SO3APTS
Beckman Coulter
1-aminopyrene-3,6,8-trisulfonate
At reducing terminus by reductive amination
488 nm
520 nm (of labeled product)
523 synthesized as tri-sodium salt
2 × 5 mg
725898
Monosaccharide-Grade APTS Carbohydrate
Labeling Reagent
Purified APTS, lyophilized with citric acid.
APTS Formula:
Excitation λ:
Emission λ:
Formula Wt:
Container:
Vial Contents:
Storage:
Shelf Life:
Contents
C16H8NO9Na3S3
488 nm
520 nm
523.39
Amber glass vial, screw cap
20 mg APTS-M, 80 mg citric acid
2-8°C (light sensitive)
3 years from date of manufacture
Index
30
CE APPLICATION KITS
NUCLEIC ACIDS
Agarose slab gel electrophoresis has long been used for the analysis of DNA and RNA. Although well established, it is time
consuming, labor intensive and only semi-quantitative. Capillary gel electrophoresis offers an automated approach to nucleic acid
analysis with on-line detection that is fully quantitative, extending the analytical capabilities of electrophoresis. Gels exist as
polymers in solution, allowing the user an environment which can easily be optimized. Polymers in solution allow for an automated
mechanism of gel loading and gel replacement. Beckman Coulter offers the following nucleic acid kits useful for the routine
analysis of oligonucleotides, viral load determination, gene expression and the quantitative analysis of DNA-protein interactions
through mobility-shift assays. CEQ™ 2000XL chemistries for DNA sequencing and fragment analysis are also described.
PART N UMBER
D ESCRIPTION
477480
eCAP™ ssDNA 100-R Kit
The eCAP ssDNA Kit is designed for the analysis of oligonucleotides,
providing a direct quantitative tool. This chemistry utilizes linear polyacrylamide in solution to form a sieving gel, which provides single base
resolution of oligonucleotides between 10 and 100 bases. The neutral
capillary provided with the kit has a stable, physically bonded
polyacrylamide coating, which eliminates electroosmotic flow (EOF)
and suppresses interactions of the sample with the capillary wall. This
chemistry utilizes a standard Tris-Borate EDTA buffer including high
concentrations of urea to prevent secondary structure formation.
Applications of this kit have included DNA and RNA probes, ribozymes
and anti-sense phosphorothioate oligonucleotides. This chemistry is
compatible with both UV and LIF detection.
0.10
0.10
0.08
0.08
0.06
0.06
0.04
0.04
0.02
0.02
The kit includes coated capillaries (2), replaceable gel, and
standards to perform at least 70 runs. Urea denaturant is included
separately, enabling reconstitution to a native replaceable gel, if so
desired.
Reorder Components
477477
0.00
0.00
15
20
25
30
35
40
Analysis of 22-mer oligonucleotide;
300 V/cm and 30°C.
Beckman Coulter
477621
45
338481
477626
Contents
eCAP Neutral Capillary (DNA) 100 µm I.D.,
65 cm total length, 12 cm from window to outlet
(quantity 1)
ssDNA 100-R Polyacrylamide Gel (lyophilized)
(quantity 1 g)
Buffer Kit, includes: Tris Borate EDTA Buffer (2)
and Urea 7 M (2)
ssDNA 100-R Test Mix, Poly d(A) 40-60 Mer
Oligonucleotides (containing 20 fragments)
(quantity 0.2 O.D. units)
Index
31
CE APPLICATION KITS
NUCLEIC ACIDS (CONTINUED)
PART N UMBER
D ESCRIPTION
477410
eCAP™ dsDNA 1000 Kit
The eCAP dsDNA 1000 Kit is designed for the analysis of doublestranded DNA fragments from 72 to 1000 base pairs. This chemistry
utilizes linear polyacrylamide in solution to form a sieving gel. A
neutral capillary provided with this kit has a stable, physically bonded
polyacrylamide coating which eliminates electroosmotic flow (EOF)
and suppresses interactions of the sample with the capillary wall. This
chemistry utilizes a standard Tris-Borate EDTA buffer and allows the
addition of intercalating dyes like ethidium bromide or our proprietary
EnhanCE™ to improve resolution and increase sensitivity. This kit is
ideal for the analysis of PCR* products which can be analyzed directly
with no sample clean-up. Applications of this kit have included gene
expression analysis (competitive PCR), and genotyping experiments.
The system is compatible with both UV and LIF detection, but LIF is
recommended for most applications.
Kit includes: (2) 100 µm I.D. neutral capillaries (DNA),
(3) vials of replaceable polyacrylamide gel, (1) test mix consisting
of HaeIII restriction digest φ174 DNA containing 11 fragments from
72 bp to 1,353 bp, and instructions and methods for system set-up and
analysis. For LIF applications, we recommend the use of the
intercalating dye EnhanCE.
* PCR is covered by U.S. patents owned by Hoffmann-La Roche Inc.
Reorder Components
477477
Separation of an Hae III restriction digest of
φX174 DNA using LIF detection. Pressure
injection for 10 seconds (10 µg/mL sample).
477628
477414
477406
eCAP Neutral Capillary (DNA) 100 µm I.D.,
65 cm total length, 12 cm from window to outlet
(quantity 1)
dsDNA 1000 Polyacrylamide Gel Kit
Lyophilized (quantity: package of 3)
dsDNA 1000 Test Mix, HaeIII Restriction Digest
φX174 DNA (quantity 20 µg)
dsDNA 1000 Internal Standard Orange G
Reference Marker (quantity 1 mL)
Recommended
477409
EnhanCE Dye (quantity 0.5 mL)
CE-LIF analysis of DNA products generated by competitive RNA-PCR of cytochrome P450-1A1 mRNA
in dioxin-treated HepG2 cells. Target sequence is
367 bp; competitor is 228 bp; CF=carboxyfluorescein
marker; IS=328 bp internal standard.
Beckman Coulter
Contents
Index
32
CE APPLICATION KITS
NUCLEIC ACIDS (CONTINUED)
PART N UMBER
D ESCRIPTION
477486
eCAP™ dsDNA 20,000 Kit
The eCAP dsDNA 20,000 Kit is designed for the analysis of doublestranded DNA fragments from 1,000 to 20,000 base pairs. This
chemistry utilizes linear polyacrylamide in solution to form a sieving
gel. A neutral capillary provided with this kit has a stable, physically
bonded polyacrylamide coating which eliminates electroosmotic flow
(EOF) and suppresses interactions of the sample with the capillary wall.
This chemistry utilizes a standard Tris-Borate EDTA buffer system and
allows the addition of intercalating dyes like ethidium bromide or our
proprietary EnhanCE™, to improve resolution and increase sensitivity.
This kit is ideal for the analysis of large PCR* products which can be
analyzed directly with no sample clean-up. The system is compatible
with both UV and LIF detection.
Kit includes: (2) 100 µm I.D. neutral capillaries (DNA),
(3) vials of replaceable polyacrylamide gel, (1) test mix consisting
of HindIII restriction digest of λ DNA. Contains 8 fragments from
125 bp to 23,130 bp. For LIF applications, we recommend the use of
the intercalating dye EnhanCE.
Reorder Components
477477
477485
477483
Before and after separation of DNA fragments
derived from the complete EcoR1 digestion of a
12 Kbp plasmid vs. the undigested plasmid itself.
477406
eCAP Neutral Capillary (DNA) 100 µm I.D.,
65 cm total length, 12 cm from window to outlet
(quantity 1)
dsDNA 20,000 Polyacrylamide Gel Kit
(lyophilized) (quantity: package of 3)
eCAP Test Mix, HindIII Digest
8 fragments from 125 to 23,130 base pairs
(quantity 500 µl)
dsDNA 1000 Internal Standard Orange G
Reference Marker (quantity 1 mL)
Recommended
477409
EnhanCE Dye (quantity 0.5 mL)
* PCR is covered by U.S. patents owned by Hoffmann-La Roche Inc.
Beckman Coulter
Contents
Index
33
CE APPLICATION KITS
C E Q ™ 2 0 0 0 X L A P P L I C AT I O N K I T S
PART N UMBER
D ESCRIPTION
608000
CEQ™ DTCS Kit
Dye-labeled dideoxy-terminator cycle sequencing kit for 96 reactions.
Includes: thermostable DNA polymerase and pyrophosphatase;
CEQ dye-labeled terminators (ddUTP, ddGTP, ddCTP, ddATP);
dNTP mix solution; sequencing reaction buffer, pUC18 control
template, M13(-47) sequencing primer, mineral oil and glycogen.
Storage temperature: -20°C.
608120
CEQ DTCS – Quick Start Kit
Dye terminator cycle sequencing (DTCS) reactions incorporate many
components including DNA polymerase, pyrophosphatase buffer,
dNTPs, dye terminators, DNA templates and primers. The CEQ DTCS
Quick Start Kit simplifies this process by using a master-mix of many of
the components—reducing the number of pipetting steps from 10 to 4
while using larger transfer volumes to reduce pipetting error. Each kit
provides enough reagents to complete 100 sequencing reactions.
608010
CEQ LFR Gel I
Special CEQ polyacrylamide denaturing gel assures high-resolution
separations on the CEQ 2000XL. Used for both DNA sequencing and
fragment analysis. Packaged in a cartridge for quick reloading by
operator. The cartridge holds 11.5 mL of polyacrylamide gel,
sufficient for 12 loads into capillary arrays (96 separations).
Storage temperature: 6°C.
608012
CEQ Separation Buffer (quantity 4/pack)
Each bottle has a twist top with a convenient dropper tip and contains
sufficient buffer (30 mL) to fill one flat-bottomed 96-well CEQ 2000XL
Buffer Plate. Storage temperature: 6°C.
608098
CEQ DNA Size Standard Kit– 400
DNA size standard for analysis of fragments up to 400
nucleotides.Sufficient for 96 fragment analysis separations. Includes
DNA fragments of the following sizes labeled with CEQ WellRED
fluorescent dye: 60, 70, 80, 90, 100, 120, 140, 160, 180, 190, 200, 220,
240, 260, 280, 300, 320, 340, 360, 380, 400, and 420 nucleotides.
Storage temperature: -20°C. Includes mineral oil for overlay.
608095
CEQ DNA Size Standard Kit– 600
DNA size standard for analysis of fragments up to 600 nucleotides.
Sufficient for 96 fragment analysis separations. Includes DNA
fragments of the following sizes labeled with CEQ WellRED fluorescent
dye: 60, 70, 80, 90, 100, 120, 140, 160, 180, 190, 200, 220, 240, 260,
280, 300, 320, 340, 360, 380, 400, 420, 440, 460, 480, 500, 520, 540,
560, 580, 600, 620, and 640 nucleotides. Storage temperature: -20°C.
Includes mineral oil for overlay.
608105
CEQ Fragment Analysis Test Sample
A set of fluorescently labeled DNA fragments of known sizes and defined
spacing used to verify the resolution power of the CEQ 2000XL fragment
analysis system. Sufficient for 24 tests. Storage temperature: -20°C.
Beckman Coulter
Contents
Index
34
CE CHEMISTRY KITS
BUFFERS, TEST MIXTURES,
PART N UMBER
AND
REAGENTS
D ESCRIPTION
338437
Capillary Performance Test Kit
The Capillary Performance Test Kit is designed to test functionality of
the CE system. The test mixture consists of low concentrations of three
closely related compounds (benzoic acid, p-hydroxybenzoic acid and
p-hydroxyphenylacetic acid—see structures). Analyzing this test
mixture confirms system resolution and sensitivity performance.
The kit contains Test Mixture A (20 mL), Run Buffer A
(50 mL), and Regenerator Solution A (20 mL). One kit is included
with each P/ACE™.
338425
Capillary Performance Test Mixture A
Benzoic acid, p-hydroxybenzoic acid, p-hydroxyphenylacetic acid
(quantity 20 mL).
OHBA
d:\ train\ cedata~1\ acids1, Channel A
0.016
0.014
BA
0.014
0.016
0.010
338424
Capillary Regenerator Solution A
0.1 N sodium hydroxide (quantity 50 mL).
0.012
PhenylAcet
0.012
338426
Capillary Performance Run Buffer A
100 mM sodium borate, pH 8.3 (quantity 50 mL).
0.010
A
U 0.008
A
0.008 U
501333
Capillary Performance Test Mix B
This test mix consists of two closely related compounds (P-OH benzoic
acid and P-OH phenylacetic acid. Analyzing this test mixture confirms
system resolution and sensitivity performance.
0.006
0.006
0.004
0.004
338436
Borate Buffer Kit
Includes boric acid solution, 0.4 M, 50 mL sodium tetraborate solution,
0.1 M (quantity 50 mL).
0.002
0.002
338433
Boric Acid, 0.4 M (quantity 50 mL)
0.000
0.000
338434
Sodium Tetraborate, 0.1 M
(quantity 50 mL)
3.0
3.5
4.0
4.5
5.0
5.5
Minutes
p-OH Phenylacetic Acid
Beckman Coulter
p-OH Benzoic Acid
Benzoic Acid
Contents
Index
35
CE CHEMISTRY KITS
CE-GRADE BUFFERS
Beckman Coulter offers an extensive line of buffers specifically
formulated for use with CE systems.
PART N UMBER
D ESCRIPTION
477422
eCAP™ Phosphate Buffer 50.0 mM, pH 2.5,
100 mL
This buffer is also contained in the following application kits:
P/N 477430 eCAP Amine Capillary Methods Development Kit
(Small Molecules) and P/N 477735 Amine Capillary Methods
Development Kit (Proteins).
477423
eCAP Phosphate Buffer 50.0 mM, pH 7.0,
100 mL
This buffer is also contained in the following applications kits:
P/N 477430 eCAP Amine Capillary Methods Development Kit
(Small Molecules) and P/N 477735 Amine Capillary Methods
Development Kit (Proteins).
477425
eCAP Acetate Buffer 50.0 mM, pH 4.5, 100 mL
This buffer is also contained in the following applications kits:
P/N 477430 eCAP Amine Capillary Methods Development Kit
(Small Molecules) and P/N 477735 Amine Capillary Methods
Development Kit (Proteins).
477426
eCAP MES Buffer 50.0 mM, pH 6.0, 100 mL
This buffer is also contained in the following applications kits:
P/N 477430 eCAP Amine Capillary Methods Development Kit
(Small Molecules) and P/N 477735 Amine Capillary Methods
Development Kit (Proteins).
477427
eCAP Tris Buffer 50.0 mM, pH 8.0, 100 mL
This buffer is also contained in the following applications kits:
P/N 501310 P/ACE™ MDQ Methods Development Kit, P/N 477430
eCAP Amine Capillary Methods Development Kit (Small Molecules)
and P/N 477735 Amine Capillary Methods Development Kit (Proteins).
477442
eCAP Citrate Buffer 20.0 mM, pH 3.0, 100 mL
This buffer is also contained in the following applications kit:
P/N 477445 eCAP Neutral Capillary Methods Development Kit
(Proteins).
Beckman Coulter
Contents
Index
36
CE CHEMISTRY KITS
CE-GRADE BUFFERS (CONTINUED)
PART N UMBER
D ESCRIPTION
477443
eCAP™ Citrate/MES Buffer (Citrate, 20.0 mM;
MES, 20.0 mM/L) pH 6.0, 100 mL
This buffer is also contained in the following applications kits:
P/N 501310 P/ACE™ MDQ Methods Development Kit and P/N 477445
eCAP Neutral Capillary Methods Development Kit (Proteins).
477444
eCAP Tricine Buffer (20.0 mM) pH 8.0, 100 mL
This buffer is also contained in the following applications kit:
P/N 477445 eCAP Neutral Capillary Methods Development Kit
(Proteins).
477447
eCAP Chiral Kit Adjustable pH Buffer
(Acetate Buffer, 50.0 mM; Phosphate Buffer,
50.0 mM/L) pH 2.5, 500 mL
This buffer is also contained in the following applications kit:
P/N 501300 P/ACE MDQ Chiral Methods Development Kit.
477448
eCAP Chiral Kit Low pH Buffer
(Phosphate Buffer, 50.0 mM) pH 2.5, 500 mL
This buffer is also contained in the following applications kit:
P/N 501300 P/ACE MDQ Chiral Methods Development Kit.
477449
eCAP Chiral Kit High pH Buffer
(Phosphate Buffer, 50.0 mM) pH 8.0, 500 mL
This buffer is also contained in the following applications kit:
P/N 501300 P/ACE MDQ Chiral Methods Development Kit.
Beckman Coulter
Contents
Index
37
LABWARE
P/ACE™ MDQ LABWARE
PART N UMBER
D ESCRIPTION
970657
Vials, 2 mL (quantity of 100)
144648
Vial Caps, 2 mL (quantity 100 red)
970668
Vials, 0.5 mL (quantity 50)
144649
Vial Caps, 0.5 mL (quantity 50 blue)
144709
PCR* Vials (quantity 50)
358821
Micro Vial Springs
Required for use of Micro Vials (quantity 10).
144656
PCR* Vial Caps (quantity 50 gray)
144657
Vial Holders
Required for use of Micro Vials (quantity 50).
373660
96-Well Plates, Nonsterile, Without Lids
Capacity: 300 µL/well (quantity 100).
144390
Buffer Tray 36 (2 mL vials)
To be used with 36 × 2 mL vial and/or vial holders.
144543
Sample Tray 48 (2 mL vials)
To be used with 48 × 2 mL vial and/or vial holders
144544
Sample Tray 48 (0.5 mL vials)
To be used with 48 × 0.5 mL vials (does not require vial holders).
144824
Large-Volume Buffer Reservoir
Consists of two buffer reservoir trays, 2 buffer reservoir lids, and lid
removal tool.
144826
Buffer Reservoir Replacement Lid
144858
Replacement Buffer Lid Removal Tool
144865
Buffer Reservoir Replacement O-Rings
(quantity 3)
P/ACE MDQ Miscellaneous Supplies
144647
Coolant Filling Kit
Consists of syringe, coolant fill tool, and connecting tubing.
359976
Liquid Coolant (450 mL, quantity 1)
* PCR is covered by U.S. patents owned by Hoffmann-La Roche Inc.
Beckman Coulter
Contents
Index
38
LABWARE
LABWARE SUPPLIES
P/ACE™ 2000–5000 SERIES
FOR
The Autosampler of the P/ACE 2000-5000 series accommodates up to
34 vials: 10 in the inner tray (for electrolytes) and 24 in the outer tray
(for samples, conditioning fluids, and electrolytes). Each vial fits into
a vial holder, then into the autosampler. Custom-made vial caps
prevent evaporation of buffers and samples during operation.
For oligonucleotide work, Mini Vials fit into the P/ACE 4 mL
vials using the Mini Vial Springs. For microvolume work, Micro Vials
fit into the 4 mL vials using the Micro Vial Springs. The countertop vial
storage rack holds up to 40 vials in holders and 20 Micro Vials.
PART N UMBER
D ESCRIPTION
358807
Glass Vials
4 mL working volume for sample and electrolyte (quantity 100).
358818
Vial Holders
Two pieces fit into P/ACE autosampler to hold sample and electrolyte
vials (quantity 10).
359079
Vial Caps (gray silicone)
Seals vials during run (quantity 50).
360004
Vial Caps
For sample storage (quantity 10).
338487
Mini Vials
For oligonucleotide work, polypropylene, 200-400 µL working volume
(quantity 100).
338488
Mini Vial Springs
Required for use of Mini Vials (quantity 50).
358819
Micro Vials
For microvolume work, polyolefin, 5-30 µL working volume
(quantity 10).
358821
Micro Vial Springs
Required for use of Micro Vials (quantity 10).
359093
Vial Storage Rack
Holds 40 vials and 20 Micro Vials in holders (quantity 1).
359980
Micro Vial Autosampler Kit
Provided with each purchase of a P/ACE 5000. Includes 100 Vials,
10 Micro Vials, 10 Micro Vial Springs, 40 Vial Holders, Vial Storage
Rack, and 50 Vial Caps.
Beckman Coulter
Contents
Index
39
CE SUPPLIES AND LABWARE
T E M P E R AT U R E - C O N T R O L L E D S A M P L E T R AY S U P P L I E S
PART N UMBER
D ESCRIPTION
727012
Vial Holder for Cooling Tray (package of 5)
727011
Ambient Vial Holder for Tray (package of 10)
727013
Polypropylene Vials, 200 µL (package of 100)
MISCELLANEOUS SUPPLIES
FOR
P/ACE™ 2000-5000
PART N UMBER
D ESCRIPTION
359976
Coolant 450 mL (quantity 1)
961142
Regulator for Nitrogen Tank
0-100 psi regulated output pressure (quantity 1).
927781
Beckman Coulter
Contents
Nitrogen Tank Quick Connector (quantity 1)
Index
40
CE SUPPLIES AND LABWARE
CEQ™ 2000XL LABWARE
PART N UMBER
D ESCRIPTION
609801
Sample Microtiter Plates (25/pack)
Package of 25 V-bottom thermocycler-compatible polypropylene plates
with 200 µL nominal volume to easily contain 40 µL of sample.
Recommended for use as the CEQ 2000XL Sample Plate.
607445
Wetting Tray
Plastic wetting tray serves as a docking station for the capillary inlet
tips when not in use. One is included with the CEQ 2000XL DNA
Analysis System.
609844
Buffer Microtiter Plates (100/pack)
Four packages of 25 each flat-bottom polystyrene plates, nonsterile,
without lids. Recommended for use as the CEQ 2000XL Separation
Buffer Plate.
974705
Gel Waste Bottle (12/pack)
Used for automatic collection of purged gel after separations on the
CEQ 2000XL. Each bottle will hold 250 mL of waste gel, which is
equivalent to approximately 50 plates of 96 runs each.
607533
Evaporation Control Mat (1/pack)
607530
Buffer Evaporation Cover Assembly (1/pack)
The buffer evaporation cover is used to control evaporation of buffer
during operation. One mat is included with the buffer evaporation cover
assembly. One cover assembly is included with the CEQ 2000XL DNA
Analysis System.
Beckman Coulter
Contents
Index
41
CUSTOMER SUPPORT
C O M P R E H E N S I V E VA L I DAT I O N
PROGRAM
With a quality management system registered to the ISO 9001 standard,
Beckman Coulter utilizes a qualified development process. The P/ACE™
series CE systems have been developed under this process to comply
with all CE, UL, and CUL mark requirements. The Software Validation
Pack provides basic information regarding development and performance testing for the system software and can be utilized to support
customer validation programs. A trained field service engineer performs
instrument installation following a prescribed installation operation
qualification protocol. In addition to installation, installation qualification includes conformity and delivery, basic training, and instrument
performance testing. Optional operation qualification programs provide
convenient processes that ensure that the instrument is performing to
published specifications using calibrated testing equipment traceable
to national standards.
PART N UMBER
D ESCRIPTION
144867
P/ACE MDQ and P/ACE 5000
Installation Qualification (IQ): For original start-up of new system.
Includes instrument unpacking, set-up, start-up installation environment.
No charge with system purchase.
144869
P/ACE MDQ OQ1
721110
P/ACE 5000 OQ1
Operation Qualification 1 (OQ1): Basic customer training and review of
tutorial guide, performance verification, noise and drift tests, and documentation. No charge with system purchase.
144870
P/ACE MDQ OQ2
721111
P/ACE 5000 OQ2
Operation Qualification 2 (OQ2): For on-going operation qualification
of a system. Performance verification, noise and drift tests, preventative
maintenance service and parts, software verification test and report, documentation, preventative maintenance checklist, service report and
instrument decal. OQ2 can be purchased as either an option to an existing service agreement or as a reimbursable service.
144688
P/ACE MDQ OQ3
726140
P/ACE 5000 OQ3
Operation Qualification 3 (OQ3): Comprehensive, full featured
operation qualification support. Can be performed at installation or
as an option to a service agreement. Includes preventative maintenance
service and parts, software verification test and report, comprehensive
function test of components, NIST standard traceability, documentation
of engineer’s qualification, test equipment calibration, NIST standards,
service report decal and preventative maintenance checklist.
Beckman Coulter
Contents
Index
42
CUSTOMER SUPPORT
Software Validation
The Software Validation Pack includes a description of intended use,
product history, technical description, algorithms, product documentation,
options, development environment, support policies and notices.
Beckman Coulter
PART N UMBER
D ESCRIPTION
149989
32 Karat™ Software Validation
144638
P/ACE™ MDQ Software Validation
726124
Software Validation for Gold™ Software
Versions 8.1 and 8.2
Contents
Index
43
CAPILLARY ELECTROPHORESIS EDUCATIONAL AIDS
PART N UMBER
D ESCRIPTION
P/ACE™ MDQ Information
CD-8296A
Glycoprotein Analysis CD*
CD-8297A
Nucleic Acid Analysis CD*
CD-8571A
Chiral Analysis CD*
BR-8177B
P/ACE MDQ Brochure
D-8239B
CE Expert
DS-8284A
Laser-Induced Fluorescence Detector Data Sheet
T-1823A
Comparison of Air and Liquid Cooling in
Capillary Electrophoresis
T-1839A
Translating Methodologies from P/ACE 2000 and
5000 to P/ACE MDQ
T-1841A
Application of the Caesar Algorithm to
Capillary Electrophoresis
T-1860A
Precision in Capillary Electrophoresis
* Ordering information available at http://www.beckmancoulter.com
Primers
Beckman Coulter
360643
Introduction to Capillary Electrophoresis
(Volume I)
266924
Micellar Electrokinetic Chromatography
(Volume III)
727484
Separation of Proteins and Peptides by Capillary
Electrophoresis: Applications to Analytical
Biotechnology (Volume V)
538703
Quantitative Applications of Capillary
Electrophoresis in Pharmaceutical Analysis
(Volume VI)
607397
Separation of DNA by Capillary Electrophoresis
(Volume VII)
725036
High Performance Capillary Electrophoresis of
Carbohydrates (Volume VIII)
AP-8508A
Capillary Electrochromatography
Contents
Index
44
CAPILLARY ELECTROPHORESIS EDUCATIONAL AIDS
PART N UMBER
D ESCRIPTION
Newsletters
Beckman Coulter
NL-8412A
P/ACE Setter. “Inaugural Edition.” Vol. 1, Issue 1,
Summer 1997
NL-8413A
P/ACE Setter. “Zen and the Art of the Capillary
Tube.” Vol. 1, Issue 2, Fall 1997
NL_8415A
P/ACE Setter. “Advances in the Quantification of
HIV-1 Viral Load.” Vol. 2, Issue 1, Winter 1998
NL_8416A
P/ACE Setter. “CE in Biotechnology: Providing
Solution-Based Assays for Well-Specified
Biologics.” Vol. 2, Issue 2, Spring 1998
NL-8417A
P/ACE Setter. “CE in Genetic Analysis: Is it a
Natural Progression ... or a New Frontier?” Vol. 2,
Issue 3, Summer 1998
B98-5182
P/ACE Setter. “Advancements in Forensic
Toxicology: CZE Replaces GC/NPD as the Screen
of Choice for Basic Drugs.” Vol. 2, Issue 4, Fall 1998
B98-3134
P/ACE Setter. “Using cIEF to Characterize
Recombinant Human Monoclonal Antibodies.”
Vol. 3, Issue 1, April 1999
NL-8320A
P/ACE Setter. “Detecting the Presence of
Abnormal Prion Protein from Blood: Clearing a
Major Analytical Hurdle.” Vol. 3, Issue 2, June 1999
NL-8408A
P/ACE Setter. “Using Binding Isotherms to
Accelerate CE Methods Development.” Vol. 3,
Issue 3, September 1999
NL_8516A
P/ACE Setter. “CE-SSCP Screening for BRCA1
and BRCA2—Allelic Mutations Associated With
Breast Cancer.” Vol. 3, Issue 4, December 1999
NL-8597A
P/ACE Setter. “Advancing Atherogenesis Research:
High-Resolution Analysis of Oxidized Low-Density
Lipoproteins.” Vol. 4, Issue 1, June 2000
NL-8721A
P/ACE Setter. “Assessing the Homogeneity of
Plasmid DNA: An Important Step Toward Gene
Therapy.” Vol. 4, Issue 2, October 2000
NL-8756A
P/ACE Setter. “Rapid Analysis of Single-Stranded
STR Multiplexes Using P/ACE™ MDQ With
Dual-Channel LIF Detection.” Vol. 4, Issue 3,
December 2000
NL-8866A
P/ACE Setter. “Applying Enantioselective CE in
the Pharmaceutical Industry.” Vol. 5, Issue 1,
April 2001
Contents
Index
45
TECHNICAL & APPLICATION INFORMATION
PART N UMBER
D ESCRIPTION
DNA Applications
A-1726A
High-Sensitivity Analysis of Nucleotides Using
Electrokinetic Injection and Sample Stacking with
MECC
A-1748A
Capillary Electrophoresis of dsDNA Fragments
with UV and Laser-Induced Fluorescence
Detection
A-1774A
Quantitative Capillary Electrophoretic Analysis of
PCR* Products Using LIF
A-1788A
Competitive RNA-PCR by CE and LIF Detection
for Quantitation of Cellular mRNA
A-1872A
Improved Sequencing of Plasmids on the CEQ
2000 by a Simple Template Preheating Procedure
A-1876A
Highly Precise DNA Sizing on the CEQ™ 2000
Fragment Analysis System
A-1879A
Analysis of 26 Weber Panel Simple Tandem
Repeat Polymorphisms Using the CEQ 2000
Fragment Analysis System
A-1892B
Detecting Heterozygotes with the CEQ 2000XL
DS-738A
Analysis of Nucleic Acid Derivatives and Analogs
by P/ACE™
R-8034
Multi-Target PCR Analysis by CE and LaserInduced Fluorescence. Nature, March 17, 1994
T-1853A
Automation of Plasmid Purification with the
Biomek® 2000 and QIAGEN’s “QIAprep 96
Turbo BioRobot” Kit for DNA Sequence Analysis
on the CEQ 2000 DNA Analysis System
T-1878A
Allele Size and Plus A in CEQ Fragment Analysis
Software
* PCR is covered by U.S. patents owned by Hoffmann-La Roche Inc.
Beckman Coulter
Contents
Index
46
TECHNICAL & APPLICATION INFORMATION
PART N UMBER
D ESCRIPTION
Pharmaceutical Applications
A-1746
Use of High-Low CE for Improved Quantitative
Determination of Drug-Related Impurities
A-1747
Quantitative Determination of Salbutamol-Related
Impurities by CE with P/ACE 2000
A-1754
Enantiomeric Analysis of Primary and Secondary
Amines
A-1859A
Advancements in Forensic Toxicology: CZE
Replaces GC/NPD as the Screen of Choice for
Basic Drugs
A-1889A
Methods Development Strategy for Enantiomer
Analysis Using the P/ACE MDQ Chiral System
DS-766
Separation of Antibiotics & Corticosteroids by
MECC
DS-821
Analysis of Flavonoids by Micellar Electrokinetic
Chromatography with P/ACE System 2000
CD-8577A
Reflections on Chirality Tutorial*
* Ordering information available at http://www.beckmancoulter.com
Protein Applications
A-1736
CE as a Micropreparative Method
A-1740
Analysis of Proteins by Capillary Zone
Electrophoresis
A-1744
The Analysis of a Recombinant Granulocyte
Macrophage Colony Stimulating Factor
A-1745B
Resolution of Glycoforms of Ribonuclease B by
P/ACE™
A-1750
Capillary Isoelectric Focusing Using
Pressure/Voltage Mobilization
A-1757
Monitoring Disulfide Bond Formation with P/ACE
Capillary Electrophoresis
A-1761
Separation of Recombinant Protein Glycoforms by
Micellar Electrokinetic Capillary
Chromatography
A-1763
Analysis of a Monoclonal Antibody-Cytotoxin
Conjugate by P/ACE Capillary Electrophoresis
with Laser-Induced Fluorescence Detection
continued on next page
Beckman Coulter
Contents
Index
47
TECHNICAL & APPLICATION INFORMATION
PART N UMBER
D ESCRIPTION
Protein Applications (continued)
Beckman Coulter
A-1769
Qualitative and Quantitative Analysis of
Monoclonal Antibodies by One-Step Capillary
Isoelectric Focusing
A-1771A
Hemoglobin Analysis by Capillary Isoelectric
Focusing
A-1772
Quantitative Capillary Electrophoresis of
Monoclonal Antibodies Using the Neutral Capillary
and SDS 14-200 Method Development Kits
A-1777A
Characterization of Proteases by CE with LaserInduced Fluorescence Detection
A-1778A
Digoxin Immunoassay Using CE with LIF
Detection
A-1791A
Quantitation of Bioactive Peptides in Serum by CE
with LIF Immunodetection (CE-LIF-ID)
A-1861A
Using cIEF to Characterize Recombinant Human
Monoclonal Antibodies
A-1870A
Detecting the Presence of Abnormal Prion Protein
from Blood: Clearing a Major Analytical Hurdle
DS-774B
Peptide Mapping with Capillary Electrophoresis
DS-824
High-Sensitivity Peptide Mapping with Capillary
Electrophoresis Using Laser-Induced Fluorescence
Detection on a P/ACE System
DS-833
Globin Separation with P/ACE CE
R-8163A
High-Resolution Carbohydrate Profiling by
Capillary Gel Electrophoresis, Nature, Vol. 380,
4/4/96, 461-462
R-8165A
Analysis of Mono-and Oligosaccharide Isomers
Derivatized with 9-Aminopyrene-1, 4, 6-trisulfonate by C.E. with Laser-Induced Fluorescence,
Analytical Biochemistry 230, 273-280 (1995)
R-8166A
Characterization of 9-Aminopyrene-1, 4, 6-trisulfonate-Derivatized Sugars by Capillary
Electrophoresis with Laser-Induced Fluorescence
Detection. Analytical Chemistry, 1995, Vol. 67, #13,
2239-2245
Contents
Index
48
TECHNICAL & APPLICATION INFORMATION
PART N UMBER
D ESCRIPTION
General Technical/Application Information Bulletins
R95-2890
Comparison of Air and Liquid Cooling in
Capillary Electrophoresis (U.S. only)
T-1842A
Mobility Determination in Capillary
Electrophoresis
Article Reprints
Beckman Coulter
AR-8571
Putting CE to Work — LC*GC
R-8035A
Determination of Salbutamol-Related Impurities
by Capillary Electrophoresis—Journal of
Chromatography, Dec. 1992
Contents
Index
49
INDEX
Numerics
32 Karat Workstation for P/ACE MDQ.........................................14
32 Karat Workstation Migration Upgrade .....................................14
A
Accessory Pack (for PC)................................................................14
Application Kits.............................................................................22
APTS Carbohydrate Labeling Reagent ...................................30
CEQ DTCS Kit........................................................................34
CEQ DNA Size Standard Kits ................................................34
CEQ DTCS Quick Start Kit ....................................................34
Chiral Highly Sulfated Cyclodextrins .....................................24
Chiral Methods Development Kit ...........................................25
eCAP Carbohydrate Labeling and Analysis Kit .....................30
eCAP cIEF 3-10 Screening Kit ...............................................27
eCAP SDS 14-200 Kit ............................................................28
eCAP Neutral Capillary Methods Development Kit...............29
eCAP ssDNA 100-R Kit..........................................................31
eCAP dsDNA 1000 Kit ...........................................................32
eCAP dsDNA 20,000 Kit ........................................................33
MDQ Chiral methods Development Kit .................................25
MDQ Methods Development Kit ............................................22
Article Reprints..............................................................................49
B
Buffers and Reagents .....................................................................35
Borate Buffer Kit.....................................................................35
Boric Acid................................................................................35
Capillary Performance Run Buffer A/B ..................................35
Capillary Regeneration Solution .............................................35
CEQ LFR Gel I .......................................................................34
CEQ Separation Buffer ...........................................................34
eCAP Acetate Buffer ...............................................................36
eCAP Chiral Kit Adjustable pH Buffer...................................37
eCAP Chiral Kit High pH Buffer............................................37
eCAP Chiral Kit Low pH Buffer ............................................37
eCAP Citrate Buffer ................................................................36
eCAP Citrate/MES Buffer.......................................................37
eCAP MES Buffer...................................................................36
eCAP Phosphate Buffer...........................................................36
eCAP Tricine Buffer................................................................37
eCAP Tris Buffer.....................................................................36
Sodium Tetraborate .................................................................35
C
D
Detector Accessories......................................................................12
488 nm Notch Filter (P/ACE MDQ) .......................................12
520 nm Bandpass Filter (P/ACE MDQ) .................................12
663 nm Bandpass Filter (P/ACE MDQ) .................................12
Detector Replacement Parts...........................................................19
EDA Cartridge Adapter Kit.....................................................19
MDQ EDA Capillary Adapter Assembly ................................19
MDQ EDA Tubing Kit ............................................................19
Detectors ........................................................................................11
MDQ DAD ..............................................................................11
MDQ Dual λ LIF.....................................................................11
MDQ External Detector Adapter Accessory Kit.....................11
MDQ Single λ LIF ..................................................................11
MDQ UV/Vis ..........................................................................11
E
Educational Aids for CE ................................................................44
F
Calibration......................................................................................13
635 LIF Calibration/Test Standard..........................................13
Capillary Arrays (CEQ 2000XL)...................................................18
CEQ DNA Separation Arrays..................................................18
Capillaries ......................................................................................15
eCAP Amine Capillary (50 µm)..............................................17
eCAP Amine Capillary (75 µm)..............................................17
eCAP N-CHO Capillary..........................................................15
eCAP Neutral Capillary ..........................................................15
eCAP Neutral Capillary (SDS-MW).......................................16
eCAP Neutral Capillary DNA.................................................16
Fused-Silica .............................................................................18
Beckman Coulter
Untreated Fused-Silica ............................................................18
Capillary Electrophoresis (Description) ..........................................4
Carbohydrate Analysis...................................................................30
Cartridges.......................................................................................19
Blank High Sensitivity UV Cartridges....................................21
eCAP Blank Capillary Cartridges ...........................................21
eCAP Capillary Replacement Kit ...........................................21
LIF Cartridge Blank ................................................................21
MDQ Aperture 100 × 200 .......................................................19
MDQ Aperture 100 × 800 .......................................................19
MDQ Blank Cartridge Assembly Kit......................................19
MDQ Blank Cartridge CE-MS/CEC Kit ................................19
MDQ Cartridge Aperture O-Rings..........................................19
MDQ Cartridge Rebuilding Kit...............................................19
MDQ Cartridge Tubing Kit .....................................................19
MDQ EDA Cartridge Assembly..............................................19
MDQ EDA Cartridge Body.....................................................19
MDQ EDA Tubing Kit ............................................................19
P/ACE Capillary Cartridge......................................................20
P/ACE High Sensitivity UV Cartridge....................................20
P/ACE LIF Cartridge...............................................................20
Cartridge Clips...............................................................................19
MDQ Dual Seal (RED Seals)..................................................19
MDQ Single Seal (GREEN Seals)..........................................19
Coolant ....................................................................................38, 40
Contents
Fiber Optic Cables .........................................................................13
LIF Fiber Optic Cable .............................................................13
LIF Fiber Optic Cable for Laser with SMA 905 Connector...13
MDQ DAD Fiber Optic Cable ................................................13
MDQ UV/Vis Fiber Optic Cable.............................................13
Replacement UV/Vis Fiber Optic Cable for P/ACE MDQ ....13
Replacement DAD Fiber Optic Cable for P/ACE MDQ ........13
Filters .............................................................................................12
UV Filters ................................................................................12
LIF 488 nm Notch Filter .........................................................12
LIF Emission Band Pass Filters ..............................................12
LIF Detector Filter Holder ......................................................12
50
INDEX
H
Highly Sulfated Cyclodextrins.......................................................24
History ..........................................................................................2-3
K
Kits—see Application Kits
L
Labware..........................................................................................38
96-Well Plates.........................................................................38
Ambient Vial Holder for Temperature-Controlled Tray) ........40
Buffer Reservoir Lid Removal Tool........................................38
Buffer Reservoir Replacement Lid..........................................38
Buffer Reservoir Replacement O-Rings..................................38
Large-Volume Buffer Reservoir ..............................................38
MDQ 36 Position Buffer Tray (2 mL Vial).............................38
MDQ Sample Tray 48 .............................................................38
Micro Vial Autosampler Kit ....................................................39
Mini and Micro Vials, Springs, Caps (P/ACE 2000-5000).....39
Polypropylene Vials for Temperature-Controlled Tray ...........40
Vials, Caps, Holders, Springs (MDQ).....................................38
Vials, Caps, Holders, Springs (P/ACE 2000-5000) ................39
Vial Holder (Temperature-Controlled Sample Tray)...............40
Vial Storage Rack (P/ACE 2000-5000)...................................39
Lamps.............................................................................................13
MDQ Deuterium Lamp Assembly ..........................................13
P/ACE 2000 Deuterium Lamp Assembly ...............................13
P/ACE 5000 Deuterium Lamp Assembly ...............................13
Laser Modules................................................................................11
488 nm Argon-Ion Laser .........................................................11
635 nm Diode Laser ................................................................11
M
Modules for P/ACE MDQ .............................................................10
N
Newsletters (P/ACE Setter)............................................................45
Nitrogen Tank Regulator................................................................40
Nitrogen Tank Quick Connector....................................................40
Nucleic Acids Application Kits .....................................................31
P
P/ACE Systems ...............................................................................6
MDQ Carbohydrate ...................................................................9
MDQ Chiral...............................................................................6
MDQ DAD Temperature Control..............................................9
MDQ DNA ................................................................................8
MDQ Glycoprotein....................................................................7
MDQ-LCQ CE-MS/MS ............................................................7
MDQ Methods Development ................................................... 6
MDQ Molecular Characterization................................................8
MDQ Quality Control .............................................................. 6
MDQ UV/Vis Ambient ............................................................ 9
MDQ UV/Vis Temperature Control......................................... 9
P/ACE 5000...............................................................................9
P/ACE 5010 UV/Vis Temperature Control ............................. 9
P/ACE 5500 DAD Ambient ..................................................... 9
Beckman Coulter
Contents
P/ACE 5510 DAD Temperature Control.................................. 9
P/ACE Education Package ....................................................... 8
Primers ...........................................................................................45
I: Introduction to Capillary Electrophoresis............................45
III: Micellar Electrokinetic Chromatography..........................45
VII: Separation of DNA by Capillary Electrophoresis ...........45
V: Separation of Proteins and Peptides by Capillary
Electrophoresis: Applications to Analytical Biotechnology ...45
VI: Quantitative Applications of Capillary Electrophoresis
in Pharmaceutical Analysis ....................................................45
VIII: High Performance Capillary Electrophoresis
of Carbohydrates .....................................................................45
Capillary Electrochromatography ...........................................45
Protein Analysis .............................................................................27
R
Reagents—see Buffers and Reagents
S
Sample Tray ...................................................................................34
MDQ 48 Position (0.5 mL Vials) ............................................34
MDQ 48 Position (2 mL Vials) ...............................................34
Software .........................................................................................14
32 Karat Software Reprocessing Key.....................................14
32 Karat Software (User Installed) .........................................14
32 Karat Software Migration Upgrade (User Installed) .........14
Standards........................................................................................27
cIEF Protein Standards Kit .....................................................27
T
Technical and Application Information .........................................46
DNA Applications ...................................................................46
General Technical/Application Information Bulletins ............49
P/ACE MDQ Applications ......................................................44
Pharmaceutical Applications ...................................................47
Protein Applications ................................................................47
Test Mixtures .................................................................................13
Capillary Performance Test Mix A/B......................................35
HS Cyclodextrin Test Mid.......................................................24
LIF Test Mix (Sodium Fluorescein)........................................13
Protein Test Mix (P/ACE MDQ).......................................22, 28
Small Molecule Test Mix ........................................................22
ssDNA 100-R Test Mix ...........................................................31
V
Validation Programs.......................................................................42
32 Karat Software Validation Pack ...................................14, 42
Gold Software Validation (Version 8.1 and 8.2) .....................43
MDQ and P/ACE 5000 Instrument
Qualification (IQ) ....................................................................42
MDQ and P/ACE 5000 Operation
Qualification 1 (OQ1) .............................................................42
MDQ and P/ACE 5000 Operation
Qualification 2 (OQ2) .............................................................42
MDQ and P/ACE 5000
Operation Qualification 3 (OQ3) ............................................42
MDQ Software Validation .......................................................43
P/ACE Station Validation ........................................................43
51
All trademarks are the property of their respective owners.
All specifications subject to change without notice.
Developing innovative solutions in genetic
analysis, drug discovery, and instrument systems.
Beckman Coulter, Inc. • 4300 N. Harbor Boulevard, Box 3100 • Fullerton, California 92834-3100
Sales: 1-800-742-2345 • Service: 1-800-551-1150 • Telex: 678413 • Fax: 1-800-643-4366 • www.beckmancoulter.com
Worldwide Bioresearch Division Offices:
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B98-519
Contents
Index