What is SilenciX®
• New strategy for Knock-Down (KD) based on EBV vector
• Main benefits:
 Efficiency
- Validation performed by qPCR
- KD level guarantee: >70%
 Specificity
 High Stability and long term culture: 3-12 months
 No off-target effects
 Non-viral genome integrity
 Safe
 Ready to Use (RTU)
SilenciX® is a registered trademark of tebu-bio, technology licensed from the CEA.
How SilenciX® works
The Epstein-Barr (EBV)-based vector is
anchored to chromosomes and replicates
as a replicon without any integration in the
genome. Reinforced by optimal
hygromycin selection, the number of
vectors is perfectly maintained.
SilenciX® is a registered trademark of tebu-bio, technology licensed from the CEA.
How SilenciX® works
•
•
•
•
Replicative vector ensures genome integrity
Capable of anchoring to chromosomes bringing stability
Hygromycin resistance reinforces EBV replication
H1-promotor expression gives efficient siRNA delivery
SilenciX® is a registered trademark of tebu-bio, technology licensed from the CEA.
Why SilenciX®
“Silence is a fence around wisdom”
Proverb
 To have access to a powerful cellular model –
fast!
 To study a physiological role of your protein in
cellular model – fast!
 To screen and analyze new therapeutics in the
early stage of their development – fast!
 To save time and money – fast!
SilenciX® is a registered trademark of tebu-bio, technology licensed from the CEA.
Quantitative PCR (qPCR) Quality Controls
 Three independent cell lines silenced for
each targeted gene of interest are
established
 The knock-down efficiency is monitored
with qPCR.
 The most efficient* knock-down (70-80%) is
selected for your experiment
 The remaining cell lines can be sent upon
request to analyze intermediate effects.
*The efficiency depends on the gene of interest
SilenciX® is a registered trademark of tebu-bio, technology licensed from the CEA.
SilenciX®: Efficiency
70-80% of knockdown leads to a very low
remaining gene expression in SilenciX,
compared to endogenous expression.
SilenciX® is a registered trademark of tebu-bio, technology licensed from the CEA.
SilenciX: Comparison to siRNA and shRNA
technologies
siRNA
Stability
2‐3days
shRNA
HeLa and/or all human and rodent cell lines
Several month, Several month
Start new experiment with different cell batch
Efficiency
Production time
Price
Reproducibility
Robustness
Integration and off‐target effect
Additional reagents*
Additional experiments
SilenciX
Start new experiment with the same cell batch: just thaw the vial
>70% validation by qPCR
Few days, controlling each experiment
~$1,200, control not included
no
5 weeks
$833 shRNA only
Final cost ~$3,000
Yes, with experience
no
no
no
yes
Required
Protocol optimization
Transfection, qPCR
Required
Protocol optimization
Transfection, Selection, qPCR
2/3days
$3,690
High reproducibility,
Homogeneous population
Highly robust
no
No additional reagents
Ready to use
(RTU)
SilenciX® is a registered trademark of tebu-bio, technology licensed from the CEA.
SilenciX® : “silencing” DUBs
Deubiquitylases (DUBs): major players in a variety of cellular processes such as
cell cycle, DNA damage response (DDR), cell proliferation, cell growth and
apoptosis
Ready to supply:
• USP7 HeLa SilenciX
• USP9x HeLA SilenciX
• USP14 HeLa SilenciX
• USP19 HeLa SilenciX
Don’t see your DUB? Custom cell lines are available.
Custom production with all attached human and rodent cell lines compatible with
lipofectamine transfection.
SilenciX® is a registered trademark of tebu-bio, technology licensed from the CEA.
Target-specific SilenciX® cell lines
Successfully silencing over 100 genes
Example: DNA repair
Base Excision Repair, Nucleotide Excision Repair, Mismatch Repair and Double-Stranded Breaks
Breast Cancer Susceptibility and Fanconi
Anemia
SLX-00027 FancD2 HeLa SilenciX
SLX-00028 BRCA2 HeLa SilenciX
SLX-00041 BRCA1 HeLa SilenciX
Non-Homologous End Joining
SLX-00010 Ligase IV HeLa SilenciX
SLX-00005 DNA-PKcs HeLa SilenciX
SLX-00018 XRCC4 HeLa SilenciX
SLX-00021 XLF HeLa SilenciX
Xeroderma Pigmentosum
SLX-00015 XPA HeLa SilenciX
SLX-00016 XPC HeLa SilenciX
SLX-00003 XPF HeLa SilenciX
SLX-00133 XPG HeLa SilenciX
Sensors/transducers
SLX-00029 ATM HeLa SilenciX
SLX-00025 ATR HeLa SilenciX
SLX-00085 PARG HeLa SilenciX
SLX-00008 KIN17 HeLa SilenciX
Homologous Recombination
SLX-00022 Rad54a HeLa SilenciX
SLX-00124 Rad54b HeLa SilenciX
Nucleotide Excision Repair
SLX-00006 HR23A HeLa SilenciX
SLX-00007 HR23B HeLa SilenciX
SLX-00002 ERCC1 HeLa SilenciX
SLX-00015 XPA HeLa SilenciX
SLX-00016 XPC HeLa SilenciX
MRN complex
SLX-00013 Rad50 HeLa SilenciX
SLX-00012 NBS12 HeLa SilenciX
SLX-00011 MRE11 HeLa SilenciX
Mismatch Repair
SLX-00023 MSH2 HeLa SilenciX
SLX-00052 PCNA HeLa SilenciX
SLX-00089 FEN1 HeLa SilenciX
Base Excision Repair
SLX-00053 NEIL1 HeLa SilenciX
SLX-00089 FEN1 HeLa SilenciX
SLX-00019 PARP1 HeLa SilenciX
SLX-00020 PARP2 HeLa SilenciX
SLX-00018 XRCC1 HeLa SilenciX
SLX-00009 Ligase III HeLa SilenciX
SLX-00014 Ogg1 HeLa SilenciX
Double-strand Breaks
SLX-00005 DNA-PKcs HeLa SilenciX
SLX-00021 XLF HeLa SilenciX
SilenciX® is a registered trademark of tebu-bio, technology licensed from the CEA.
SilenciX® at work
Examples of custom
SilenciX cell lines
SilenciX works in MRC5-V2 (human fibroblasts) and
MCF7 (beast cancer cell line) with specific knockdown
efficiency up to 80%.
Other cell lines are compatible with SilenciX
technology:
HeLa: Human immortal cell line from cervical cancer
cells
HEK293: Embryonic Kidney 293 cell line
Caco-2: Epithelial colorectal adenocarcinoma cells
AC16: Proliferating cardiomyocyte cell line
SH-SY5Y: Neuroblastoma cell line
RKO: Colorectal carcinoma cell line
HCT116: Colon Cancer cell Line
U2OS: Osteosarcoma cell line
B16F10: Murine melanoma cells
CHO: Chinese hamster ovary cells
XPA/XPC - zinc finger proteins involved in DNA excision
repair
KIN17- RNA-binding protein, involved in DNA replication
and the cellular response to DNA damage
MRE11 – DNA double-strand break repair protein
SilenciX® is a registered trademark of tebu-bio, technology licensed from the CEA.
SilenciX® : What do you get
For each cell lines, you receive :
 One vial of target specific SilenciX® cells
 One vial of control SilenciX® cells (transfected with a nonrelevant shRNA sequence
 Specification sheets with qPCR quality controls and details of
shRNA sequence
 Complete user manual
SilenciX® is a registered trademark of tebu-bio, technology licensed from the CEA.