SCREENING FOR PROS TAT E DISE A SE S
:
CPSE :
A p owe r f u l to o l fo r d eve lo p i n g
yo u r g e r i a tr i c s e r v i ce
A s p e c if i c m a r ke r f o r p r o s t a t e d i s e a s e
ODELIS ® CPSE,
> CPSE (Canine Prostatic Specific arginine Esterase) is a hormone
that is secreted by the prostatic epithelial cells,
under the control of the sex hormones.
Indications for use:
> Confirm or exclude a diagnosis of BPH and rapidly adapt the treatment.
> CPSE is present in sperm (more than 90% of the proteins
in the prostatic fluid) and in blood.
> A simple method for the early screening of BPH, from the first signs.
> This protein belongs to the family of kallikreins, like PSA in man.
> A simple and reliable alternative to rectal examination, which is often poorly
tolerated by the animal and their owner.
> When the prostatic cells become hyperplastic, the serum
concentration of CPSE increases (4).
Now,
there is a n easier
a n d m o r e p r e c i s e m et h o d …
> Justify the performance of additional examinations and exclude other
concomitant diseases (cysts, abscess, prostatitis, etc.).
A RANGE OF SERVICES AND DOCUMENTS
TO ASSIST THE VETERINARIAN
The serum concentration of CPSE is an excellent indicator
of benign prostatic hyperplasia in the dog (5) (6) (7)
Poster and client information sheets:
to inform your clients about the importance of regular screening in elderly dogs.
PROS TAT E DISE A SE
1600
More than
Poster 40x60
and client information sheet
800
affected!
*
New methods are now available
to detect prostate disease
200
Tests are distributed by:
100
50
0
*
*
*
BPH
N=34
CPSE concentration as a function of clinical status: results from 89 dogs (7)
BVT-ADV 3 volets A4 fermé-GB.in1 1
Ask your veterinarian
for more information
2009 - Photos © Droits réservés
400
elderly dogs
are
2009 - Photos : © Droits réservés
CPSE (ng/mL)
of
*
Normal
N=48
Prostatitis
N=5
Laboratory
tests
Adenocarcinoma
N=5
Diagnostics at the Veterinarian’s Service
www.bvt.fr
20/04/09 17:09:06
:
Benign Prostatic hyperplasia (BPH):
The Performance of
The fir st ELISA test to screen
and diagnose BPH
A co m m o n co n d i t i o n
w i t h s e r i o u s co n s e q u e n c e s
A study was undertaken in 89 un-castrated male dogs in collaboration with the CERCA – Maisons
Alfort (France) and the CRECS – L’Isle Jourdain (France)
BPH CAN HAVE SERIOUS CLINICAL CONSEQUENCES (1) :
Vertebral column
Prostate
Bladder
Urethra
Description of the sample population:
55 dogs diagnosed without BPH*
34 dogs diagnosed with BPH*
(From Fontbonne and al. 2006 (9))
• Constipation, tenesmus
• Dysuria, haematuria
• Pain, lameness, and behavioural problems
• Infertility in breeding dogs
• Haematospermia
* The BPH / non-BPH status was determined following clinical examination, ultrasonography, and histology for some dogs
Clinical status
BPH
Non-BPH
33
4
1
51
Testicles
Diagram : Photostock of GERES
Odelis® CPSE
The increased volume of the prostate leads to compression of the rectum, urethra,
blood vessels, and nerve fibres.
Sensitivity = 97,1% / Specificity = 92,7 %
THE HISTOLOGICAL LESIONS
PRECEDE THE SYMPTOMS (2,3)
HOW DO YOU INTERPRET THE RESULTS OF
ODELIS ® CPSE ?
Over 50% of dogs over 5 years of age present
with histological signs of early BPH.
An asymptomatic BPH may progress rapidly and predispose
the animal to more serious conditions
(prostatitis, voluminous cysts, prostatic abscesses etc.).
≥ 61 ng/mL
< 61 ng/mL
Clinical signs suggestive of prostate disease:
Prostatic hyperplasia (© E. Teske)
> Odelis® CPSE enables your laboratory to perform a quantitative assay of CPSE from a simple
serum or plasma sample.
Stud dog
Dogs > 5 years
and / or
• Constipation
• Dysuria, haematuria
• Pain, lameness, behavioural problems
• Infertility, haematospermia
> The serum or plasma sample can be stored for 24h at room temperature or 96 hours at +4°C,
enabling the sample to be sent to the laboratory by courier service.(6)
REGULAR SCREENING IS
RECOMMENDED IN ELDERLY DOGS
Recommended by numerous authors (7,8) to:
> Provide the client with clear information about
the consequences of this disease,
> Delay the onset of symptoms through appropriate
monitoring and treatment,
> Closely monitor at-risk dogs: stud males, elderly dogs,
those with a previous history of BPH, etc
Odelis® CPSE Test
Plasma
5.
6.
7.
8.
9.
Verstegen J. Managment of prostatic disorders . In : Proceedings of the International WSAVA Congress, Granada 2002- Spain
Barsanti J. Canine prostatic disease. in Proceedings of the International SCIVAC Congress, Rimini 2008- Italy
Johnston SD, Kustritz MVR, Olson PNS. Benign prostatic hypertrophy/hyperplasia. In: Canine and feline theriogenology. WB Saunders, Philadelphia. 2001:340-341
Chapdelaine P, Dubé JY, Frenette G et coll. Identification of arginine esterase as the major androgen-dependent protein secreted by dog prostate and preliminary molecular
characterization in seminal plasma. J Androl. 1984 ; 5 : 206-210
Bell FW, Klausner JS, Hayden DW et coll. Evaluation of serum and seminal plasma markers in the diagnosis of canine prostatic disorders. J Vet Intern Med. 1995 ; 9 :149-153
Etude interne BVT
Lévy X, Mimouni P. Hyperplasie bénigne de la Prostate : actualités. Point Vétérinaire. 2009. 293, 39-43
Romagnoli S. Medical importance of benign prostatic hyperplasia for the long term health of the dog. In : Prostate technical booklet, 2008, Virbac
Fontbonne et al. Reproduction canine Ed. Royal Canin, 2006
BVT-ADV 3 volets A4 fermé-GB.in2 2
No clinical signs
(sample collected
into a heparin tube)
© F.Decante
Transfer of the serum
or plasma to a plain
tube
Blood sample
1.
2.
3.
4.
Normal CPSE concentration
< 50 ng/ml
PROCEDURE FOR SENDING A SAMPLE FOR CPSE ASSAY TO A LABORATORY
(<1 hour)
Serum
(sample collected in a plain tube)
Courier service
to the laboratory *
Presence of
clinical signs
BPH unlikely
it is advisable to put an ice block
in with the sample
* The samples were stable after
24 hours at room temperature,
96 hours at +4°C, and several
months at -20°C.
Repeat test in 6
to 12 months
Consider different
differential diagnoses
Intermediate CPSE concentration
entre 50 et 70 ng/ml
No clinical signs
Presence of
clinical signs
BPH possible
Repeat test in 3
to 6 months
Raised CPSE
concentration
> 70 ng/ml
Strong suspicion
of BPH
Prostatic ultrasonography recommended:
evaluation of other associated diseases
20/04/09 17:09:21