1. health and fitness
2. disease

20140613a.pdf
IR-4 Ornamental Horticulture Program
Research Report Cover Sheet
Trial: 2013 CA
Researcher(s) Cai-Zhong Jiang
University of California
Affiliation:
CA
PhoneNumber:
Email:
ProjectTitle:
Protocol #:
PR#
31894
31569
31562
31572
31563
31564
31571
31565
31566
31568
Botrytis Efficacy
13-017
Research Target
Crop/Plant
Product
EPA Reg. # Production Site
Botrytis Gray Mold
Rose
BAS703 01F
Botrytis cinerea
Rosa sp.
Fluxapyrosad + pyraclostrobi BASF
-
Botrytis Gray Mold
Rose
Disarm 480SC
66330-64
Botrytis cinerea
Rosa sp.
Fluoxastrobin
OHP
Status
Greenhouse
C
Greenhouse
C
Greenhouse
C
Botrytis Gray Mold
Rose
F9110
-
Botrytis cinerea
Rosa sp.
F9110
FMC
Botrytis Gray Mold
Botrytis cinerea
Rose
Rosa sp.
Medallion
Fludioxonil
100-769
Syngenta
Greenhouse
C
Botrytis Gray Mold
Rose
Palladium
100-1328
Greenhouse
C
Botrytis cinerea
Rosa sp.
Cyprodinil + fludioxanil
Syngenta
Botrytis Gray Mold
Botrytis cinerea
Rose
Rosa sp.
Proud 3
Thyme oil (5.6%)
Greenhouse
Biohumanetics
C
84059-3
Greenhouse
C
Greenhouse
C
Botrytis Gray Mold
Rose
Regalia 5O (MOI-10605)
Botrytis cinerea
Rosa sp.
Extract of Reynoutria sachali Marrone Bio In
Botrytis Gray Mold
Rose
SP2770 10WP
-
Botrytis cinerea
Rosa sp.
SP2770
SePro
Botrytis Gray Mold
Botrytis cinerea
Rose
Rosa sp.
SP2773
SP2773
SePro
Greenhouse
C
Botrytis Gray Mold
Rose
V-10135
-
Greenhouse
C
Botrytis cinerea
Rosa sp.
Fenpyrazamine
Valent
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
p1
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
Narrrative Summ
mary (Resullts/Discussion)
Plea
ase keep tex
xt to one pag
ge if possible
e. Include su
ummary of tri
rial results an
nd a brief disscussion inccluding how
any changes from the protoc
col may hav
ve affected re
esults. Resu
ults for multip
ple PRnumb
bers can be ssummarized
d
toge
ether, but ple
ease list all PRNumbers
P
in the heade
er and in the
e summary d
data table.
Dise
ease Rates
disease rate
es, refer to Tables 1 and 2 and Figurres 1 and 2. BAS703 (P
PR#31859) w
was shown to
For d
dram
matically reduce the amo
ount of funga
al infections in miniature
e rose flowerrs through th
he course of the
expe
eriment for both
b
applicattion rates of 4 oz per 100
0 gal and 8 o
oz per 100 g
gal, howeverr the statisticcal
signiificance for the
t differenc
ce in mean disease
d
rating went awayy on Day 28
8 for the 4 ozz per 100 ga
al BAS703
rate (see notes below).
b
Palladium (PR#
# 31563), Pro
oud 3 (PR# 31564), SP2
2770 (PR# 3
31565), SP2
2773 (PR#
3156
66, and V10135SC (PR#
# 31568) acttually showe
ed a few stattistically sign
nificant incre
eases in dise
ease rates
versus the untre
eated controls plants, esp
pecially after Day 21, bu
ut we believe
e that after D
Day 14, the sshelf-life of
the fflowers have
e expired and
d the flowers
s rapidly bec
come infecte
ed with fungii after senesscence.
Phyttotoxicity
Therre found no evidence
e
of phytotoxicity
y from any of
o the treatm
ments. Althou
ugh there were differencces in the
chan
nge of growth between treatments, the
t variability
y was also h
high on Day 0 between g
groups (Figu
ure 4 and
Figure 5). There
e was no lea
af necrosis or
o leaf yellow
wing, except for mild leaff yellowing d
due to senesscence at the
e
e of most of the plants affter Day 14, but that occ
curred even in control plants. We te
ested for premature
base
sene
escence as an
a indicator of phytotoxicity by keep
ping track of “Pale” flowe
ers, but the d
data was sevverely
impa
acted by sec
condary fung
gal disease that
t
afflicted senescing fflowers.
es:
Note
1). M
Multiple attem
mpts were made
m
to reac
ch the Cleary
y Chemical rrepresentativve for the sa
ample of Torrque
fungicide (PR# 31567)
3
but th
hey could no
ot be reache
ed, so Torqu e was not in
ncluded in th
his study.
Data collecte
ed at Day28 should be in
nterpreted with caution b
because the shelf life of the minirose
e flowers
2). D
was terminated and
a result in
n dead flowe
ers.
Ornamenta
al Horticultu
ure Program
m
Research Report Fo
orm
Researc
cher:
Project Title:
Protoco
ol #:
p2
Cai-Zh
hong Jiang Date:
6/6/2014
Efficacy
cy of Management Tools for Botryttis
13-017
PR numbers: 31894, 31569, 31562, 31572, 315563, 31564, 315771, 31565, 31566,, 31568
Results
s Table
Please iinsert results tab
ble here. Includ
de PRnumbers for
f each treatme
ent if multiple PR
Rnumbers are in
included in this ssummary. Pleasse include
product,, active ingredie
ent, and statistic
cs.
Click he
ere to enter text..
Mean Total Dis
sease Rating (±S
SD)
PR #
31894
31894
31569
31562
31572
31563
31564
31571
31565
31566
31568
Product
Active Ingredient
Control
-BAS703 half
Xemium an
nd pyraclobstrin
BAS703 full
Xemium an
nd pyraclobstrin
Disarm
Fluox
xastrobin
F9110
F9110
F
Medallion
Fludioxonil
Palladium
Cy
yprodinil
Proud 3
Thyme
e oil (5.6%)
Regalia
Extract of Reyn
noutria sachalinen
nsis
SP2770
SP
P2770
SP2773
SP
P2773
V10135SC
Fenpy
yrazamine
Day 0
Day 7
Day 14
Day 21
Day 28
0.0 (± 0)
0.0 (± 0)
0.0 (± 0)
0.0 (± 0)
0.0 (± 0)
0.0 (± 0)
0.0 (± 0)
0.0 (± 0)
0.0 (± 0)
0.0 (± 0)
0.0 (± 0)
0.0 (± 0)
0.0
0 (± 0)
0.0
0 (± 0)
0.0
0 (± 0)
0.0
0 (± 0)
0.0
0 (± 0)
0.0
0 (± 0)
0.0
0 (± 0)
0.0
0 (± 0)
0.0
0 (± 0)
0.0
0 (± 0)
0.0
0 (± 0)
0.0
0 (± 0)
4.1 (±
± 0.5)
± 1.0)
*1.6 (±
± 0.4)
*0.9 (±
4.6 (±
± 0.9)
2.4 (±
± 1.5)
5.3 (±
± 1.7)
4.2 (±
± 2.3)
3.9 (±
± 1.0)
5.9 (±
± 2.4)
5.7 (±
± 2.0)
± 0.9)
*5.3 (±
4.5 (±
± 1.8)
7.5 (± 1.00
*4.6 (± 1. 7)
*3.7 (± 1.44)
8.1 (± 1.00)
6.8 (± 1.44)
8.7 (± 0.44)
*9.3 (± 0. 9)
8.4 (± 0.66)
7.9 (± 2.22
8.7 (± 1.66)
8.5 (± 0.99)
9.0 (± 1.66)
8.4 (± 1.1)
6.0 (± 2.5)
*6.4 (± 1.4)
9.0 (± 1.4)
8.5 (± 1.0)
9.5 (± 0.6)
9.4 (± 0.4)
*9.7 (± 0.6)
9.4 (± 0.6)
*9.9 (± 0.2)
*9.8 (± 0.3)
*9.8 (± 0.6)
Mean Growth (cm)
4.8
7.2
8.6
4.5
6.9
2.6
3.2
1.4
3.1
3.8
4.1
-0.7
Table 1:: Overall summa
ary of total disea
ase rating, where 0 = no sign of
o disease and 10
1 = 100% of flo
ower blooms affflicted with eithe
er black petals or
o
ed
powderyy fungal growth.. Bolded and as
sterisks values show results with statistical sig
gnificance when compared to untreated controls. Data collecte
at Day28 should be inte
erpreted with ca
aution because almost
a
all flowers senesced an
nd the shelf-life o
of the minirose were terminated
d.
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
BA
AS703 half B
BAS703 full Disarm F9110 M
Medallion P
Palladium Proud 3 Regalia SP2770 SP2773 V
V10135SC p3
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
Day 14 *0.002 *0.000 0.286 0.068 0.179 0.907 0.714 0.163 0.147 *0.043 0.669 Day 21
1 *0.016
6 *0.002
2 0.355 0.365 0.051 *0.017
7 0.105 0.717 0.198 0.141 0.119 Day 28 0.106 *0.0
041 0.470 0.839 0.081 0.113 *0.0
046 0.100 *0.0
032 *0.0
041 *0.0
044 Table 2: Confide
ence intervalls of the mea
ans between
n each treatm
ment group and the con
ntrol group ussing
Stud
dent’s T-test.. P ≤ 0.05. Bold
B
and astterisked valu
ues indicate statistical siignificance. Days 0 and
d 7 are not
show
wn because there was no variance in all treatme
ents on thos e data collecction dates. Notes: Data
a collected at
Day2
28 should be
e interpreted
d with caution because almost
a
all flo
owers senesced and the shelf-life of the
minirrose were te
erminated.
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
Day 14
V10135SC
SP2773
SP2770
Regalia
Proud 3
Palladium
Medallion
F9110
Disarm
BAS703 full
BAS703 half
Control
Treatment
Treatment
Day 0 a
and 7
0%
20%
40%
60%
80%
V100135SC
SSP2773
SSP2770
Regalia
PProud 3
Pallladium
Meedallion
F9110
Disarm
BAS7703 full
BAS7703 half
C
Control
100%
0%
an proportion of Mea
affflicted flowerss
Treatment
20%
40%
40%
60%
80%
100%
Day 28
V10135SC
SP2773
SP2770
Regalia
Proud 3
Palladium
Medallion
F9110
Disarm
BAS703 full
BAS703 half
Control
0%
20%
portion of
Mean prop
afflicted flowers Day 2
21
Treatment
p4
60%
80%
an proportion of
Mea
affflicted flowerss
100%
V100135SC
SSP2773
SSP2770
Regalia
PProud 3
Pallladium
Meedallion
F9110
Disarm
BAS7703 full
BAS7703 half
C
Control
0%
20%
40%
60%
80%
100%
owers
Meaan proportion of afflicted flo
Figure 1: Repres
sentation of the average
e proportion of flowers th
hat exhibited
d each of the
e four health states
dery (red), Black
B
(green), and Pale (purple).
scored: Healthy (blue), Powd
Ornamenta
al Horticultu
ure Program
m
Research Report Fo
orm
Cai-Zh
hong Jiang Date:
6/6/2014
Efficacy
cy of Management Tools for Botryttis
13-017
PR numbers: 31894, 31569, 31562, 31572, 315563, 31564, 315771, 31565, 31566,, 31568
BAS70
03 full
8.0
6.0
4.0
2.0
0.0
10.0
8.0
6.0
4.0
2.0
0.0
7
4
14
21
28
7
DAIIA
14
21
4.0
2.0
0.0
2.0
21
28
8.0
6.0
4.0
2.0
14
DAIA
6
6.0
4
4.0
2
2.0
0
0.0
0
7
14
21
28
0
7
21
28
4
14
21
28
21
28
DAIIA
Regalia
10.0
8.0
6.0
4.0
2.0
0.0
7
8
8.0
Proud 3
10.0
0
10
0.0
DAIA
0.0
DAIIA
4.0
28
Total disease rating
Total disease rating
6.0
4
14
6.0
Pallad
dium
8.0
7
8.0
DAIA
10.0
0
10.0
0.0
0
Medallion
F9110
Total disease rating
0
Total disease rating
Disarm
Total disease rating
10.0
Total disease rating
Total disease rating
BAS703 half
Total disease rating
Researc
cher:
Project Title:
Protoco
ol #:
p5
10
0.0
8
8.0
6
6.0
4
4.0
2
2.0
0
0.0
0
7
14
DAIA
21
28
0
7
4
14
DAIIA
Ornamenta
al Horticultu
ure Program
m
Research Report Fo
orm
Researc
cher:
Project Title:
Protoco
ol #:
Cai-Zh
hong Jiang Date:
6/6/2014
Efficacy
cy of Management Tools for Botryttis
13-017
PR numbers: 31894, 31569, 31562, 31572, 315563, 31564, 315771, 31565, 31566,, 31568
SP27
773
8.0
6.0
4.0
2.0
0.0
V10135SC
V
10.0
Total disease rating
10.0
Total disease rating
Total disease rating
SP2770
8.0
6.0
4.0
2.0
0.0
0
p6
7
4
14
DAIIA
21
28
10.0
8.0
6.0
4.0
2.0
0.0
0
7
14
DAIA
21
28
0
7
14
21
28
DAIA
Figure 2
2: Mean plant to
otal disease ratin
ngs for each of the correspondiing treatments compared
c
to un
ntreated controlss throughout the
e course of the
e
experim
ment. Evaluation
n was based on the percentage
e of flowers on each
e
plant that had
h either visiblle powdery fung
gus or blackened petals. These
two prop
portions were ad
dded together and
a converted to
o a 0-10 scale to
o determine the
e total disease ra
ating, where 10
0% afflicted flow
wers equals a
rating off 1, 20% afflicted flowers equals a rating of 2, etc.
e
Standard errors
e
are plotted.
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
Mean Plant Height
30
Height (cm)
Height (cm)
25
20
15
10
Day 0
5
Day 28
0
Treatmentt
Figure 3: Mean plant
p
height on Day 0 an
nd Day 28 fo
or each treattment.
Mean ch
M
hange in
n height (cm)
10.0
Change in height (cm)
Change in height (cm)
p7
8.0
6.0
4.0
2.0
Change
0.0
‐2.0
Horizontaal
p
height on
o Day 0 and
d Day 28.
Figure 4: Change in mean plant
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
erials & Metthods/Reco
ordkeeping
Mate
Plea
ase fill out the
e information
n below or attach
a
a sepa
arate docum
ment with com
mparable infformation.
Nam
me(s) of Pers
sonnel Conducting Rese
earch: Chao Ma, Ayla Norrris, Nick Daoo, Gulden Hasspolat, Daide Che
Loca
ation of Trial (city/state): Davis, CA
Use Site (greenh
house/shade
ehouse/field container/etc): Greenhoouse
Crop
p History
Crop Cultiva
ar/Variety:
Date of
stick cutting
g:
Date of
first cut / fin
nal cut:
Date of
first space / final space:
Potting Mix:
Pot size & spacing:
s
Row spacin
ng:
Karina miniature
m
rose
e
Decemb
ber 4, 2013
Decemb
ber 25, 2013 / January 15, 2014
January
y 22, 2014 / Ja
anuary 29, 20
014
Peat mo
oss perlite mix
x
4” pot, 5 pots per 10’ x 20’ tray
N/A
Prod
duct(s) applie
ed prior to sttart of experriment:
Product
Date of
Application
Bonzi
Bonzi
Bonzi
Overture
Flagship
p8
1-31-2014
2-17-2014
2-19-2014
1-28-2014
2-26-2014
A
Add more rows as neede
ed.
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
p9
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
Expe
eriment Info
ormation
E
Experimenta
al Design:
N
Number of Reps:
R
Comple
etely Random
mized
5
M
Materials & Methods:
M
IInsert materiials & metho
ods here only
y if informatiion is not pre
esented else
ewhere.
IInclude any changes
c
from
m protocol.
nt Material
Plan
Immature potted
d miniature ro
oses of the Karina
K
varie
ety were retriieved from th
he Rocket F
Farms nurserry (Rocket
Farm
ms, Half Moo
on Bay, CA) on 2-26-201
14 and place
ed indoors u
under grow liights with a light strength
h of 18 µmol
m2s-1. The flowe
ers were uno
opened in tig
ght buds. On 3-3-2014 tthe plants w
were moved tto the greenhouse. The
e
plantts were allow
wed to acclim
mate for one
e week beforre the first trreatment and
d data collecction date on
n 3-10-2014
(Dayy 0). By thatt point the flo
owers were fully
f
open an
nd mature. Plants rema
ained in the 4
4’ pots they were
delivvered in. The plants werre brought in
ndoors for th
he final three
e days of the
e experimentt to avoid exxposure to a
routine greenhou
use pesticide application
n that was sc
cheduled.
Trea
atments
The plants were arranged fiv
ve pots to a tray, one tra
ay for each o
of the 11 trea
atments, plu
us one untrea
ated control
tray. On each data collectio
on day, the pesticides
p
we
ere prepared
d according to the instru
uctions and ssprayed onto
o
the fflowers and leaves until wet using ha
andheld spra
ay bottles. T
The untreate
ed control plants were allowed to
ase bias due
rema
ain dry. Also
o, each week the tray po
ositions were
e rearranged
d on the ben
nch to decrea
e to possible
e
light and temperrature gradie
ents in the grreenhouse.
Dise
ease scoring
g
On e
each collection date (Day 0 (3-10-14
4), Day 7 (3-17-14), Dayy 14 (3-24-14
4), Day 21 (3
3-31-14), and Day 28 (47-20
014) the num
mber of flowe
ers on each plant
p
display
ying each off the four hea
alth categoriies (Healthy, Powdery,
Blacck, and Sene
escent were counted. Healthy flowe
ers were brig
ght red with n
no sign of disease or senescence.
Pow
wdery flowers
s had visible white, powd
dery fungus on one or m
more petals, which may b
be powdery mildew or
mold
d. Black flow
wers had one
e or more pe
etals that had blackened
d petals that resemble B
Botrytis infection.
Flow
wers that had
d both blacke
ened petals and powderry fungus we
ere categorizzed as Powd
dery. Senesscent flowers
s
had pale flowers
s. The numb
ber of flowerrs in each ca
ategory was converted in
nto the perce
entage of the total
number of flowers on each plant,
p
which was then co
onverted to a 0-10 scale
e, where 0 eq
quals 0% affflicted and
10 equals 100% afflicted. For the total disease
d
scorre, Powderyy and Black sscores were added toge
ether. Also
at ea
ach collectio
on date, plants were evaluated for ph
hytoxicity sy mptoms succh as leaf bu
urning / yello
owing or
stuntted growth. Paling sene
escent flowe
ers were included in the e
evaluations because pre
emature sen
nescence
can be a sympto
om of phytoto
oxicity.
nt Height
Plan
On D
Day 0 (3-10--2014) and Day
D 28 (4-7-14), plant he
eight was me
easured at tthe plants’ highest pointss.
Statistical Analysis
Statiistical significance betwe
een disease rating mean
ns between the controlss and the trea
atments wass calculated
using
g Student’s t test through JMP softw
ware. Standard errors w
were plotted o
on the graph
hs using Miccrosoft
Exce
el.
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
p 10
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
Appllication Equipment:
handheld spray
s
bottles
s
P
Product(s) applied during
g experimen
nt (including treatments, fertilizers, e
etc):
Product
Active
ingredie
ent
Rate(s)
Applicatio
on
Type
Date o
of
Appliccation
op Growth
Cro
Sta
age
Application
n
Volume
BAS703
Xemium
m and
pyracolb
bstrin
8 oz per 100
ga
al
Foliar
*
Matture
20 mL
BAS703
Xemium
m and
pyracolb
bstrin
4 oz per 100
ga
al
Foliar
*
Matture
20 mL
Disarm 480S
SC
F9110
Medallion
WDG
Palladium
Proud 3
Fluoxasttrobin
F9110
Fludioxo
onil
Cyprodin
nil
Thyme oil
o
(5.6%)
Extract of
o
Reynouttria
sachalin
nensis
8 oz per 100
32
2l oz per 100
l per 100
8 oz
l per 100
6 oz
4 qts per 100
ga
al
4 oz per 100
ga
al
Foliar
Foliar
Foliar
Foliar
Foliar
**
*
**
*
*
Matture
Matture
Matture
Matture
Matture
20 mL
20 mL
20 mL
20 mL
20 mL
Foliar
*
Matture
20 mL
SP2770
SP2773
Fenpyra
azamine
Hoaglan
nd
solution
2.66 lbs per
10
1.00
66 lbsl per
10
00
l 100
16
6 oz per
l
1%
%
Foliar
Foliar
Foliar
Sub-irrigatiion
*
*
*
Every 4
4-5
days
20 mL
Matture
Matture
Matture
20 mL
20 mL
20 mL
--
Regalia
SP2770 10W
WP
SP2773
V10135SC
Fertilizer
*Applied every 7 day
ys: Day 0 (3--10-14), Day
y 7 (3-17-14)), Day 14 (3--24-14), and
d Day 21 (3-3
31-14).
**Applied
d every 14 days: Day 0 (3-10-14)
(
an
nd Day 14 (3
3-24-14), acccording to prrotocol.
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
p 11
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
Phottos
Plea
ase embed photos
p
here or
o send jpg, tiff, or bmp.
Figure 5: The arrea where we
e conducted
d the experim
ment. Photo
o taken on Day 15 after iinitial applica
ation.
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
p 12
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
Figure 6: An exa
ample of a ro
ose flower sc
cored as “He
ealthy.” Pet als are brigh
ht red and firrm, with no ssign of
disea
ase or senes
scence.
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
p 13
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
Figure 7: This flo
ower had da
arkening peta
als even beffore the bloo
om fully open
ned. It is like
ely infected w
with
Botry
rytis. It was scored
s
as “B
Black.”
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
p 14
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
Figure 8: A fully opened flow
wer with exte
ensive blacke
ening of pet als. Scored
d as “Black.”
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
p 15
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
Figure X:
er afflicted with
w a powde
ery fungus, likely powderry mildew. ““Powdery” flo
owers like th
his were
Figure 9: A flowe
uently accom
mpanied by one
o or more
e blackened petals. Anyy flower posssessing pow
wdery funguss was scored
d
frequ
as “P
Powdery” reg
gardless of additional
a
black/pale pettals.
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
p 16
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
Figure 10: Additional examp
ple of a powd
dery flower which
w
is beg inning to bla
acken.
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
p 17
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
Figure 11: Exam
mple of a flow
wer scored as
a “Pale.” Th
hese flowerss show pale discoloration as they be
egin to
sene
esce, but dis
splay no sign
ns of infectio
ous disease.
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
p 18
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
Figure 12: Pale flowers
f
with visible powd
dery fungus were scored
d as “Powde
ery.”
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
p 19
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
Figure 13: Micro
oscope image of a rose petal
p
with po
owdery funga
al growth.
Ornamenta
al Horticultu
ure Program
m
Research Report Fo
orm
Researc
cher:
Project Title:
Protoco
ol #:
p 20
Cai-Zh
hong Jiang Date:
6/6/2014
Efficacy
cy of Management Tools for Botryttis
13-017
PR numbers: 31894, 31569, 31562, 31572, 315563, 31564, 315771, 31565, 31566,, 31568
e
Figure 1
14: Photographs
s of an example
e plant from each treatment group on each data
a collection date
e. No photogra
aphs were taken
n on Day 7 since
100% off the flowers in every
e
treatmentt group were he
ealthy like on Da
ay 0. See attach
hed .jpeg file forr a larger, highe
er resolution piccture.
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
p 21
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
Data
a Collected
Plea
ase describe data collectted and scorring system. Also include
e the dates data were collected.
Heallth Ratings
The days, expre
essed in the figures
f
as Days
D
after initial applicati on (DAIA) w
were as follow
ws: Day 0 = 3-10-14,
Day 7 = 3-17-14
4, Day 14 = 3-24-14,
3
Day
y 21 = 3-31-14, and Dayy 28 = 4-7-20
014. The percentage of flowers per
plantt were categ
gorized into four
f
health groups
g
(Healthy, Powde ry, Black, an
nd Pale, desscribed in the
e Methods
sectiion) which was
w then con
nverted to a 0-10 scale, where
w
0 is 0
0% afflicted a
and 10 is 10
00% afflicted
d. Total
disea
ase rating was
w calculate
ed by adding
g the Powder and Black rating.
nt Height
Plan
On D
Day 0 (3-10--2014) and Day
D 28 (4-7-14), plant he
eight was me
easured at tthe plants’ highest pointss.
Enviironmental data
Each
h day, the weather and temperature
t
were record
ded. The grreenhouse iss known to b
be about 50%
% humidity
everry day.
w Data
Raw
Insert raw data below
b
or sen
nd separate file containin
ng raw data..
(Sen
nt as separatte .xlsx file)
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
p 22
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
Environmental conditions
c
du
uring the exp
periment:
Insert temperatu
ure, precipita
ation and/or irrigation, an
nd relative h umidity with
h a minimum
m of high, low
w and
mperatures. Or send se
eparate file with
w this inforrmation.
averrage daily tem
Inclu
ude a statem
ment about any significan
nt weather or
o environme
ental events during the ccourse of the
e
expe
eriment.
Tempe
erature
90
85
Temperature (F)
Temperature (F)
80
75
70
65
Low
60
High
55
Median
50
45
40
0
5
10
15
20
25
30
Day
Figure 15: Low, high, and median daily temperature
t
es in the gree
enhouse wh
here the expe
eriment wass conducted.
Orrnamental Horticulture Pro
ogram
Researc
ch Report Form
Rese
earcher:
Project Title:
Prottocol #:
p 23
Cai-Zhong Jiang
J
Date:
6/6/2014
Efficacy of Management
M
Tools
T
for Botrrytis
13-017
PR num bers: 318944, 31569, 315662, 31572, 311563, 31564,
31571, 311565, 31566, 31568
Day
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
Date
3/10/2014
3/11/2014
3/12/2014
3/13/2014
3/14/2014
3/15/2014
3/16/2014
3/17/2014
3/18/2014
3/19/2014
3/20/2014
3/21/2014
3/22/2014
3/23/2014
3/24/2014
3/25/2014
3/26/2014
3/27/2014
3/28/2014
3/29/2014
3/30/2014
3/31/2014
4/1/2014
4/2/2014
4/3/2014
4/4/2014
4/5/2014
4/6/2014
4/7/2014
Low
(F)
66
69
68
71
64
64
65
62
64
66
64
65
64
64
62
68
69
69
68
68
64
65
65
64
63
69
68
68
68
High
(F)
80
80
82
78
82
82
85
85
80
85
84
84
85
85
83
81
80
81
79
80
81
81
80
86
82
85
68
68
68
Me
edian
(F)
73
7
74.5
75
7
74.5
73
73
75
7
73.5
72
7
75.5
74
7
74.5
7
74.5
7
74.5
7
72.5
7
74.5
7
74.5
75
7
73.5
74
7
72.5
73
7
72.5
75
7
72.5
77
68
68
68
We
eather
Irrigation
su
unny
w
water
su
unny
su
unny
su
unny
su
unny
w
water
su
unny
su
unny
su
unny
su
unny
w
water
su
unny
su
unny
su
unny
su
unny
w
water
su
unny
su
unny
clou
udy/rain
rrain
clo
oudy
w
water
rrain
rrain
su
unny
rrain
w
water
rrain
su
unny
su
unny
rrain
w
water
Ind
doors
Ind
doors
ind
doors
er data and irrigation dattes. No unusual weathe
er occurrences happened during the
e course of
Table 3: Weathe
experiment. On Days 26-28, the pla
ants were moved indoorrs to avoid ro
outine green
nhouse pestiicide spray.
this e