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Cellular Antigen Stabilising Reagent
TransFix/EDTA
Cerebrospinal Fluid
(CSF) Sample Storage
Tubes
Instructions for Use
Version: 02, 05/2013
Product Description
TransFix® is a patented stabilisation solution that prevents cellular degradation in cerebrospinal fluid (CSF) for
analytical testing purposes.
TransFix/EDTA CSF Sample Storage Tubes consist of 5ml tubes containing 0.2ml of TransFix/EDTA.
Principle and Intended Use of TransFix/EDTA CSF Sample Storage Tubes
The active components of TransFix stabilise leukocytes and leukocytic antigens for up to 10 days. Using these
tubes to stabilise CSF leukocytes it is possible to determine cell subsets via flow cytometric analysis, up to 10
days after collection. Refer to reference for a detailed explanation of how to conduct the flow cytometric
analysis (available on www.cytomark.co.uk).
Procedure
1.
On receipt of the CSF specimen, record the volume of the sample and immediately transfer to the
TransFix/EDTA CSF Sample Storage Tube.
N.B. CSF samples should be transferred into TransFix/EDTA CSF Sample Storage Tubes
immediately after lumbar puncture, but failing this, CSF must be less than 2 hours old when it is
treated with TransFix/EDTA. Do not refrigerate the sample before treatment with
TransFix/EDTA.
2.
3.
4.
5.
6.
7.
8.
Mix gently by inversion at least 10 times and store at 2-8°C for up to 10 days.
Allow the stabilised CSF sample to return to room temperature (18-25°C) before preparing it for
flow cytometric analysis.
Add 2ml of sterile filtered phosphate buffered saline (PBS), pH 7.4 to the stabilised CSF sample and
mix gently as before.
Centrifuge the sample at 540g for 5 minutes at room temperature.
Remove the supernatant and discard.
Re-suspend the cell pellet with 300µl of sterile filtered PBS, pH 7.4.
Examine the sample e.g. using flow cytometry evaluation.
N.B. The dilution factor – depending on the volume of CSF sample - must be accounted for when
cell counts from stabilised CSF samples are recorded.
N.B. Cross linking of proteins may occur when using TransFix/EDTA. We therefore recommend
that all antibody conjugates are validated in association with TransFix/EDTA prior to routine use.
Samples of TransFix/EDTA CSF Sample Storage Tubes are available on request.
Version: 02, 05/2013
Ordering Information
Product Code
TF-CSF-5-2
TF-CSF-5-10
TF-CSF-5-25
TF-CSF-5-50
Product
TransFix/EDTA CSF Sample Storage Tubes
TransFix/EDTA CSF Sample Storage Tubes
TransFix/EDTA CSF Sample Storage Tubes
TransFix/EDTA CSF Sample Storage Tubes
(2 x 5ml tubes)
(10 x 5ml tubes)
(25 x 5ml tubes)
(50 x 5ml tubes)
Quantity
2 tubes
10 tubes
25 tubes
50 tubes
Storage Conditions and Stability
TransFix/EDTA CSF Sample Storage Tubes must be stored at 2-8°C, but must not be frozen and have a shelf life
of one year from the date of manufacture or until the expiration date on the label. A certificate of analysis can
be provided with every TransFix/EDTA CSF Sample Storage Tube product.
Reference
Sandra Quijano et al, Journal of Clinical Oncology, Vol. 27, March 2009, 1462-1467
Identification of Leptomeningeal Disease in Aggressive B-Cell Non-Hodgkins Lymphoma: Improved sensitivity
of Flow Cytometry
Warning Statements
1. TransFix/EDTA CSF Sample Storage Tubes are for research use only.
2. Do not use the TransFix/EDTA CSF Sample Storage Tubes beyond the expiration date on the box
and tubes.
3. Incubation times or temperatures other than those specified may give erroneous results.
4. TransFix/EDTA is a clear green liquid. Do not use the TransFix/EDTA CSF Sample Storage Tubes if
there is a change in the physical appearance of the liquid as this may indicate deterioration and
will affect test results.
5. TransFix/EDTA treated specimens and all materials coming into contact with them should be
handled as if capable of transferring infection and disposed of with the proper precautions.
6. Avoid contact of TransFix/EDTA and TransFix/EDTA treated samples with the skin and mucous
membranes. Any contact should be washed off with cold water immediately.
7. TransFix/EDTA does not contain any antimicrobial reagents. Microbial contamination should be
avoided or erroneous results may occur.
Cytomark is a division of Caltag Medsystems Ltd, a company registered in the UK, company
number 4162330.
Caltag Medsystems Ltd
Whiteleaf Business Centre
11 Little Balmer
Buckingham
MK18 1TF
UK
Tel: +44 (0) 1280 827460
Fax: +44 (0) 1280 827466
Email: [email protected]
www.cytomark.co.uk
Version: 02, 05/2013
Lot
Reference
No. de.
cat.
Referencia
Codice
Κωδικός
Αριθμός
Nr
Katalogow
y
Kόd
Recherche
uniquement
La
investigación
sólo utilizan
Uso da
pesquisa
somente
Utilizzare solo
la ricerca
Έρευνα
χρησιμοποιούν
μόνο
Badania
używać tylko
Výzkum
používejte
pouze
F
ES
PT
I
GR
PL
CZ
Αριομός
Πορτίδος
Kat. Nr.
Forschung
verwenden Sie
nur
D
Šarže
Lotto N⁰
N⁰ de lot
N⁰ lot
N⁰ de lot
Ch-B
Lot
Cat. No.
Research use
only
GB
Lot
Code No.
Research use
only
US
Language
Version: 02, 05/2013
Expirace
Termin
Waznosci
Ημερομπνίο
Αήεής
Sceadenza
Validade
Caducidad
Date de
peremption
Verw. bis
Exp. Date
Exp. Date
Skladováni
Przechowywa
nie
Αττοθήκενοη
Conservare
Armazenar
Almacenaje
Stockage
Lagerung
Storage
Store
Zdravi
Skodilivy
Szdodliwy
Εττίκίνδυνο
Nocivo
Prejudicial
Dañino
Dangereux
Schädlich
Harmful
Harmful
Nepoužívejte
opakovaně
Nie używać
ponownie
Μην
ξαναχρησιμο
ποιήσετε
Non
riutilizzare
Não reutilize
Nahlednete do
navodu k pouziti
Odwolaj sie do
instrukcji obstugi
Výrobce
Producent
Κατασκευασ
τής
Fabbricante
Vedere le istruzioni
per l’uso
Συμβουλευτείτε τίς
oδηγίες xρήσης
Fabricante
Fabricante
Consulte instruçŏes
de utilizaçäo
Consultar las
instrucciones de uso
Fabricant
Suivre les
instructions
d’utilisation
Ne pas
réutiliser
No vuelva a
usar
Hersteller
Manufacturer
Manufacturer
Beachten Sie die
Gebrauchsanleitung
Consult Instructions
for use
Consult Instructions
for use
Nicht
wiederverwen
den
Do not reuse
Do not reuse