The Journal of Nutrition Nutritional Epidemiology Adherence Rates to the Mediterranean Diet Are Low in a Representative Sample of Greek Children and Adolescents1,2 Meropi D. Kontogianni,3 Nikoletta Vidra,3 Anastasia-Eleni Farmaki,3 Stella Koinaki,3 Katerina Belogianni,3 Stavroula Sofrona,4 Flora Magkanari,4 and Mary Yannakoulia3* 3 Department of Nutrition and Dietetics, Harokopio University, 17671 Athens, Greece and 4‘‘Aristeides Daskalopoulos’’ Foundation, 15125 Athens, Greece Abstract Data from studies in pediatric samples exploring adherence to the Mediterranean diet are scarce. The aim of the present work was to explore adherence to a Mediterranean diet pattern in a representative sample of Greek children and age. Information on participants’ sociodemographic, anthropometric, and lifestyle characteristics were collected through telephone interviews. Adherence to the Mediterranean diet guidelines for adults and to the general dietary guidelines for children was evaluated using KIDMED scores: the higher the score, the more favorable the dietary pattern. The Goldberg cut-off limits for the ratio of energy intake:basal metabolic rate were used to evaluate dietary underreporting and children were accordingly classified as low energy reporters (LER) or non-LER. Only 11.3% of children and 8.3% of adolescents had an optimal KIDMED score ($8). In adolescents, partial correlation analysis revealed a negative weak association between KIDMED and BMI (r ¼ 20.092; P ¼ 0.031), which remained significant in the non-LER subgroup (r ¼ 20.137, P ¼ 0.011). Multiple regression analysis revealed that higher KIDMED scores were associated, in non-LER children, with less time spent on sedentary activities (P ¼ 0.002) and higher paternal education (P ¼ 0.050), whereas in adolescents, with younger age (P ¼ 0.001), less time spent on sedentary activities (P ¼ 0.015), higher maternal education (P ¼ 0.014), and higher eating frequency (P ¼ 0.041). In conclusion, low adherence rates to the Mediterranean diet were observed in Greek children and adolescents; this evidence needs to be further explored regarding its impact on health and disease. J. Nutr. 138: 1951–1956, 2008. Introduction The term Mediterranean diet has been widely used to describe the traditional dietary habits of people in Crete, South Italy, and other Mediterranean countries during the decade of the 1960s (1). The diet is characterized by abundant plant foods (fruits, mainly as typical daily desserts, vegetables, bread, other forms of cereals, beans, nuts, and seeds). It also includes olive oil as the principal source of fat, moderate amounts of dairy products (principally cheese and yogurt), low to moderate amounts of fish and poultry, red meat in low amounts, and wine consumed in low to moderate quantities, normally with meals (2,3). The Mediterranean dietary pattern is characterized as rich in monounsaturated fatty acids, a balanced ratio of (n-6):(n-3) essential fatty acids, and high amounts of fiber and antioxidants, such as vitamins E and C, resveratrol, polyphenols, selenium, and glutathione (1). Greater adherence to the traditional Mediterranean diet has been associated with a significant reduction in total mortality and improvement in longevity (4) as well as lower 1 Supported by the ‘‘Aristeides Daskalopoulos’’ Foundation. Author disclosures: M. D. Kontogianni, N. Vidra, A.-E. Farmaki, S. Koinaki, K. Belogianni, S. Sofrona, F. Magkanari, and M. Yannakoulia, no conflicts of interest. * To whom correspondence should be addressed. E-mail: [email protected]. 2 incidence of atherosclerosis, coronary heart disease, metabolic syndrome, and biochemical indicators of insulin resistance, inflammation, or cardiovascular disease risk (5–8). During the past few decades, there has been a gradual abandonment of this dietary pattern by the inhabitants of the Mediterranean basin (9), especially by the younger generations (10,11). Traditional food choices are also changing due to the increasing affluence and the progressive globalization of food supply (12). In Spain, for example, energy-dense foods high in saturated fat and low in micronutrients have replaced traditional foods and they now constitute a large part of young people’s diet, thus contributing to obesity and increasing cholesterol concentrations (13). Studies in children and adolescents exploring potential associations between either adherence to the Mediterranean diet or intake of some of its beneficial compounds and biochemical indices or clinical problems, such as obesity, are scarce (14–17). Recently, a group of investigators from Spain developed an index (KIDMED index) to assess compliance with the Mediterranean diet by young people (2–24 y old) (11) and found that greater values of this index, suggesting greater adherence to the Mediterranean diet, were associated with greater nutritional adequacy, especially in terms of vitamins and minerals (18). 0022-3166/08 $8.00 ª 2008 American Society for Nutrition. Manuscript received 28 January 2008. Initial review completed 4 March 2008. Revision accepted 26 July 2008. 1951 Downloaded from jn.nutrition.org by guest on October 6, 2014 adolescents. The study sample (n ¼ 1305, 3–18 y) was representative of the Greek pediatric population in terms of sex and The aim of the present work was to explore adherence to the Mediterranean diet based on the KIDMED index in a representative sample of Greek children and adolescents and to evaluate potential associations with sociodemographic, anthropometric, and lifestyle characteristics. Methods Assessment of sociodemographic characteristics. Parents or adolescents provided information regarding the subject’s age and nationality, weight, height, family status, as well as parental occupation and education level. Parental educational level was divided into 3 groups (1): low [illiteracy, primary, junior middle school (#9 y)] (2); medium [senior middle school (10–12 y)]; and (3) high [university or higher education ($13 y)]. From each participant’s reported weight and height, BMI was calculated (kg/m2) and participants were then categorized as underweight, normal weight, overweight, or obese, according to the International Obesity Task Force BMI cut-off points (26,27). Assessment of dietary intake and eating behavior. Dietary intake was assessed through the KIDMED questionnaire and a 24-h recall of the previous day’s dietary intake. The KIDMED index was developed in an attempt to combine the Mediterranean diet guidelines for adults as well as the general dietary guidelines for children (such as breakfast skipping) in a single index. It was based on the principles sustaining the Mediterranean dietary pattern as well as on those that undermine it. The index comprises 16 yes or no questions (11). Questions denoting a negative connotation with respect to the Mediterranean diet were assigned a value of 21 and those with a positive aspect 11. The total score ranged from 24 to 12 and was classified into 3 levels: $8, optimal Mediterranean diet; 4–7, improvement needed to adjust intake to the Mediterranean diet; and #3, very low diet quality. The 24-h recall procedure has been used in several pediatric nutrition surveys (28,29). In children, as well as in adults, there is no perfect method of assessing dietary intake. On a group basis and given that the group size is adequate, the 24-h reported intake can provide a reasonably accurate estimate of mean energy and macronutrient intake, because 1952 Kontogianni et al. Physical activity assessment. Leisure-time physical activity was estimated through a recall of the time children spent on specific activities the previous day, namely 1) walking (e.g. to school/tuition centers, etc.); 2) games or sports with friends/schoolmates (e.g. basketball, volleyball, soccer, chasing, hide-and-seek, skipping rope, etc.); and 3) organized physical exercise (e.g. basketball, volleyball, soccer, etc.). Moreover, the time spent on sedentary activities (e.g. TV viewing, computer or video games) during the previous day was also recorded. Both physical and sedentary activities were expressed in min/d. Statistical analysis. Continuous variables are presented as means 6 SD and categorical variables as absolute frequencies. The chi-square test evaluated associations between the categorical variables (such as KIDMED groups and parental education levels) and the Student’s t test and the ANOVA were applied to evaluate differences in mean values of normally distributed data between boys and girls or the KIDMED groups. Furthermore, to compare high and medium to low KIDMED groups, post hoc analyses were performed. The Bonferroni rule was used to account for the inflation of type-I error. Associations between the KIDMED score and other variables were evaluated by Spearman correlation coefficients. Partial correlations between the KIDMED and the BMI, after controlling for age, sex, and time spent in sedentary and physical activities, were also performed. Multiple linear regression analyses were applied to evaluate 5 Abbreviations used: BMR, basal metabolic rate; EF, eating frequency; EI, energy intake; LER, low energy reporters. Downloaded from jn.nutrition.org by guest on October 6, 2014 Study population. Data were collected through telephone interviews from May to July 2007. Multistage, stratified, and proportional random sampling was applied: multistage and stratified per geographical region and age group (3–12 y old, 13–18 y old), proportional to children’s and adolescents’ population aged 3–18 y per region (for urban and rural areas according to 2001 census) (19), random with regard to the household selection in each stratum, as well as the selection of the child/ adolescent in question within each household (a random-digit dialing procedure was used). At the beginning of the telephone call, potential participants were informed about the objectives and methods of the study, understood that their participation was voluntary, were assured about the confidentiality of the data, and provided their oral consent. Of the total potential participants contacted, 10,192 were ineligible (i.e. no children in the household, mismatching with age, sex, and geographical distribution required, etc.); 3545 participants refused to participate at any stage (including those who refused before being informed that were eligible for this study). The total sample consisted of 1305 participants, 3–18 y old (52% girls), 751 children (mean age: 7.6 6 2.9 y, 51% boys) and 554 adolescents (mean age: 15.5 6 1.6 y, 43.7% male). The sample was representative of the Greek pediatric population in terms of sex and age and covered all the geographical areas of Greece except the Ionian and Aegean islands. The study was approved by the Ethics Committee of Harokopio University. Interviews were conducted with either the parent (or any other person responsible for a child’s diet) for children 3–12 y old or the adolescent for participants older than 12 y. Parents or caregivers provide accurate and reliable information concerning younger children’s dietary, lifestyle, and anthropometric information; however, as children grow older, they appear to be able to recall their own intake (20–24), although a parental report still remains a better indicator of obesity than an adolescent report of weight status (25). means are robust and unaffected by the within-person variation; however, the recall technique is not appropriate for estimating actual intake on an individual basis (30,31). In our study, the interviewers were intensively trained to record, in the most detailed way, the type of food consumed (e.g. in terms of fat content, brand name, constituents of mixed dishes, etc.) as well as the quantity or volume using common household or other measures (rulers, pack of playing cards, computer mouse, etc.). For each food item or food group, specific instructions were given to the interviewers regarding the information required for the food description and the possible ways of recording quantity or volume. Special attention was paid to the day of the recall so that weekdays and weekends were proportionally represented in the total sample of recalls. Energy and macronutrient intake, meal patterns, as well as diet energy density were derived from the 24-h dietary recall. Data from recalls were analyzed for their macro- and micronutrient content by the Nutritionist Pro, version 2.2 software (Axxya Systems-Nutritionist Pro) through a hand coding procedure. The Nutritionist Pro food database was expanded by adding analyses of traditional Greek foods and recipes (3,32,33) and nutrient information of local processed food items (mainly snack foods, sweets, and fast foods) provided by the industry. Energy density was computed as total energy intake (EI)5 to total food weight consumed (kJ/g). Particular care was given to include all liquids consumed and reported by the participants, including water, beverages, and soups. However, the possibility that some beverages (especially tap water) were not reported cannot be eliminated. Dietary recalls were used to determine the number of eating episodes per day, i.e. eating frequency (EF). An eating episode was defined as any eating occasion when food or drink was consumed. The definition of an eating episode also included drinks (i.e. soft drinks or coffee) consumed in the absence of food. Two eating episodes occurring within a 15-min period or less counted as a single episode. For the assessment of low energy reporting, the ratio of the reported EI:basal metabolic rate (BMR) was determined for each subject, as in previous studies (34). The BMR was estimated using the Schofield predictive equations (35), adopted by the 2004 FAO/WHO/UNU report (36). Participants with EI:BMR # 0.89 were classified as low energy reporters (LER) based on the cut-off limits developed by Goldberg et al. (37) for 1-d recall. Normal energy reporters or non-LER were participants with EI:BMR $ 0.90. Furthermore, other aspects of eating behavior were evaluated, including dietary habits related to food consumption (frequency of at least 1 family meal per day, eating without watching television, etc.). Involvement in slimming diets either at the time of the study or in the past was also recorded. the explanatory ability of several sociodemographic and lifestyle characteristics in relation to KIDMED score (dependent variable), namely age (y), sex (female vs. male), leisure-time physical activities (min/d), sedentary activities (min/d), mother’s and father’s education level (high vs. low/ medium), EF (no. of eating episodes per day), and eating at least 1 daily family meal (yes vs. no). The results from the regression models are presented as standardized b coefficients. The level of significance was defined at P , 0.05. Data were analyzed by using the Statistical Package for the Social Sciences (SPSS 13.0) software. Results TABLE 1 Sociodemographic, dietary, and lifestyle characteristics according to age and KIDMED groups1 Children, 3–12 y Adolescents, 13–18 y KIDMED ,3 n (%) Age, y Male sex, % BMI, kg/m2 EI, kJ/d Carbohydrate intake, % energy Protein intake, % energy Fat intake, % energy Energy density, kJ/g LER,3% Sedentary activities, min/d Leisure-time physical activities, min/d Father's education level Low-medium, % High, % Mother's education level Low-medium, % High, % 1 2 3 4–7 KIDMED $8 P2 ,3 0.05 0.10 0.33 0.95 0.16 0.01 0.63 ,0.001 0.27 0.01 0.37 150 (27.0) 15.9 6 1.5 47.7 21.5 6 4.0 7289 6 3479 42.8 6 10.4 15.3 6 4.9 42.7 6 9.1 6.3 6 2.1 36.8 104.0 6 99.3 72.1 6 104.2 358 (68.6) 15.4 6 1.6* 42.5 21.1 6 3.1 7511 6 2897 42.9 6 9.2 15.8 6 3.9 42.2 6 8.2 5.4 6 1.7*** 30.7 83.3 6 79.4* 82.8 6 88.9 46 (8.3) 15.2 6 1.8* 41.3 20.5 6 2.7 7637 6 3366 43.3 6 10.3 17.1 6 5.6* 41.0 6 8.4 5.0 6 1.3*** 26.7 76.4 6 65.0 101.4 6 92.7 4–7 P2 $8 112 (14.9) 8.2 6 2.7 58.9 18.0 6 3.5 7331 6 2278 43.8 6 8.1 14.9 6 3.4 42.1 6 8.1 6.3 6 1.7 9.1 82.3 6 62.2 111.3 6 107.0 554 (73.8) 7.5 6 2.9* 48.7 18.0 6 4.0 7314 6 2269 42.4 6 9.0 16.3 6 4.0** 42.1 6 7.8 5.4 6 1.3*** 6.4 63.9 6 60.3** 108.0 6 99.5 85 (11.3) 7.5 6 3.0 55.3 17.4 6 2.8 7226 6 2918 43.9 6 9.1 16.1 6 4.0 41.3 6 7.3 5.0 6 0.8*** 8.5 55.9 6 51.0** 124.5 6 96.5 69.5 30.5 57.0** 43.0** 44.6* 55.4* 0.01 63.5 36.5 66.5 33.5 43.3 56.7 0.05 69.1 30.9 59.1** 40.9** 47.9*** 52.1 *** 0.02 68.9 31.1 68.0 32.0 45.2 54.8 0.03 0.01 0.53 0.14 0.71 0.96 0.05 0.49 ,0.001 0.29 0.02 0.16 Values are means 6 SD or percentages. Asterisks indicate different from the low KIDMED group (Bonferroni corrected): *P , 0.05, **P , 0.01, ***P , 0.001. P between all groups as derived from ANOVA or Chi-square test, respectively. Individuals with the ratio of EI:BMR #0.89 (37). Mediterranean diet in children and adolescents 1953 Downloaded from jn.nutrition.org by guest on October 6, 2014 The BMI of the children was 17.9 6 3.8 kg/m2 with 18.2% of the subjects being overweight and 12.9% obese, whereas among adolescents, the BMI was 21.2 6 3.4 kg/m2, with 13.5% of them overweight and 2.8% obese. Seven percent of the children and 32.1% of the adolescents were classified as LER. The KIDMED score was 5.4 6 1.8 in children and 4.8 6 2.1 in adolescents; only 11.3% of children and 8.3% of adolescents had an optimal KIDMED score ($8). In children, KIDMED scores did not differ between boys (5.4 6 1.9) and girls (5.4 6 1.7), but in adolescents, they tended to be higher in females (4.9 6 2.1) than in males (4.6 6 2.0) (P ¼ 0.07). Children scoring low in the KIDMED index spent more time on sedentary activities compared with those scoring medium or high (Table 1). Additionally, the educational level of their parents was lower compared with the other 2 groups, but their energy density was higher. In adolescents, those in the low KIDMED score group were significantly older and their energy density was higher compared with participants of the other 2 groups. In both children and adolescents, BMI did not differ among the 3 KIDMED score groups. In the total sample of children and adolescents, KIDMED scores were correlated (Spearman) with intakes of calcium (r ¼ 0.187; P , 0.001), vitamin C (r ¼ 0.146; P , 0.001), and fiber (r ¼ 0.073; P ¼ 0.010), and the associations remained after adjusting for low energy reporting. Correlation coefficients between BMI and KIDMED score were not significant among children, even when the analysis was restricted to non-LER. Conversely, among adolescents, the KIDMED score was weakly but significantly negatively associated with BMI (r ¼ 20.136; P ¼ 0.001) and this association remained significant when the analysis was restricted to non-LER (r ¼ 20.190; P , 0.001). Partial correlations between KIDMED and BMI, after taking into account variables such as age, sex, and time spent on sedentary and leisuretime physical activities, remained among children, whereas among adolescents, the correlations were reduced but still significant both in the total sample (r ¼ 20.092; P ¼ 0.031) and in the non-LER subgroup (r ¼ 20.137; P ¼ 0.011). Demographic, eating behavior, and physical activity factors, known for their potential effect on food choices, were then explored in regard to KIDMED. Time spent in sedentary activities (standardized b ¼ 20.128; P ¼ 0.001), maternal education level (standardized b ¼ 0.090; P ¼ 0.039), and EF (standardized b ¼ 0.085; P ¼ 0.031) was associated with KIDMED score in children (Table 2). When analyses were restricted to the non-LER, EF and maternal education level were not significant (P ¼ 0.164 and 0.094, respectively), whereas paternal education was positively associated with KIDMED score (standardized b ¼ 0.088; P ¼ 0.050). Among adolescents, variables associated with the KIDMED score were age (standardized b ¼ 20.117; P ¼ 0.010), female sex (standardized b ¼ 0.084; P ¼ 0.050), time spent in leisuretime physical activities (standardized b ¼ 0.090; P ¼ 0.045), time spent in sedentary activities (standardized b ¼ 20.133; P ¼ 0.002), and EF (standardized b ¼ 0.200; P , 0.001) (Table 2). In the non-LER subgroup analysis, age (P ¼ 0.001), time spent on sedentary activities (P ¼ 0.015), and EF (P ¼ 0.041) remained significant, whereas mother’s education level (P ¼ 0.014) was also associated with KIDMED score. TABLE 2 Results of the multiple regression models evaluating associations between subjects’ characteristics and KIDMED score1 Non-LER2 Total sample Variable Standardized b n ¼ 727 20.128 0.090 0.074 0.001 0.039 Standardized b P n ¼ 620 20.129 0.002 0.077 0.094 0.086 0.088 0.050 0.031 0.164 0.001 0.510 0.297 0.085 n ¼ 521 20.117 0.084 0.090 0.010 0.050 0.045 0.058 n ¼ 331 20.184 0.035 0.057 20.133 0.080 0.002 0.117 20.131 0.156 0.015 0.014 0.200 ,0.001 0.112 0.041 1 The table presents the results from the final models. Apart from the variables presented above, eating at least 1 daily family meal was also included as an independent variable, but it was not significantly associated with KIDMED score. 2 Individuals with ratio of EI:BMR $0.90 (37). Discussion Studies examining adherence to the Mediterranean diet among young people are scarce. Serra-Majem et al. (11) found that in a sample of 3850 children and young people aged 2–24 y residing in Spain, 46.4% had an optimal Mediterranean diet. In the present study, only 11.3% of children and 8.3% of adolescents reported eating habits following the principles of the Mediterranean diet. Hence, our findings support previous evidence for low to moderate adherence to the traditional dietary patterns among younger generations in Mediterranean countries (10,11). Unfortunately, estimates of dietary changes from longitudinal data or results from past studies examining children’s adherence to the Mediterranean diet are not available. Data from studies in adults, however, point out that diets of Mediterranean populations are gradually moving away from the traditional and beneficial patterns (9,38,39). Several factors have contributed to this nutrition transition, such as the enhanced commercial availability of food, the urbanization of life, and the overall improvement in socioeconomic conditions in Europe, which has increased the food and energy supply and made food (especially of animal origin) more affordable (40,41). Moreover, a stressful lifestyle, less time spent on cooking, and the enhanced variety and availability of household appliances have also been proposed as determinants of nutrition transition (40). Finally, the increase in migration and tourism observed in the last decades has allowed cultural and lifestyle interchanges across populations (42). Parental education level was a significant predictor of the KIDMED score. Data from large epidemiological studies support a positive independent effect of household education on the quality of children’s diet (43–45). Higher education level may be related to higher income and, thus, greater availability of healthy foods, increased nutrition knowledge, or increased motivation to follow a healthy lifestyle. In support of the latter, we found that a less sedentary lifestyle was also related to greater ad1954 Kontogianni et al. Downloaded from jn.nutrition.org by guest on October 6, 2014 Children Sedentary activities, min/d Mother's education level, high vs. low/medium Father's education level, high vs. low/medium EF, eating episodes/d Adolescents Age, y Female sex Leisure-time physical activities, min/d Sedentary activities, min/d Mother's education level, high vs. low/medium EF, eating episodes/d P herence to the Mediterranean diet, as previously evidenced in adults (46,47). Consequently, we hypothesize that behaviors related to healthy lifestyle tend to cluster and offer additional beneficial protection against degenerative diseases. Adoption of the Mediterranean diet has been associated with favorable effects on lipoprotein levels, endothelium function, insulin resistance, and metabolic syndrome (6–8). For body weight and obesity status, however, some investigators observed an inverse association between Mediterranean dietary pattern and BMI (48,49), whereas others found no correlation (50). In our analysis, the partial correlation coefficients between KIDMED and BMI were significantly negative only among adolescents. Our study has both strengths and limitations. The findings are strengthened by the fact that the sample was representative of the Greek population in terms of sex and age, covering most of the geographical areas. Furthermore, in the evaluation of dietary intake, low energy reporting was considered and all multivariate analyses were performed in the total sample as well as in normal energy reporters. The prevalences of low energy reporting found here are comparable to those evidenced in earlier studies (51,52) and the lower prevalence of low energy reporting among children as compared with adolescents may be partly attributed to the use of parents or caregivers as accurate reporters of children’s EI (53). The method of data collection, i.e. through telephone interviews, might be a potential study limitation, because direct measures of dietary intake, weight, and height were not available. On the other hand, the use of telephone surveys as a method for collecting dietary information has several advantages compared with face-to-face interviews, because they are less expensive and can reach a larger number of individuals, even in rural areas (54). The increasing need to have access to dietary information from large samples in an affordable fashion has expanded the practice of telephone dietary surveys in several population groups, including children and adolescents (55), and validation studies have supported the accuracy of the data collected (56). Adherence to a Mediterranean dietary pattern was evaluated through the use of a newly developed dietary quality index. Although the Mediterranean diet indices have a number of limitations and problems in their use, grouping foods to obtain scores is a very useful method to evaluate overall diet (57). The KIDMED was inspired by instruments previously developed for adult populations (11), because there are no specific guidelines with regards to the Mediterranean diet for non-adult populations. However, the validity of the KIDMED index has not been determined compared with other measures of dietary patterns such as FFQ. Our analysis revealed that the KIDMED score was positively associated with calcium, vitamin C, and fiber intake. These nutrients are characteristic of the food groups assessed by the score (dairy products, fruits, vegetables, pulses, nuts, and cereals) and the findings may provide some support for the validity of the index. However, the ability of KIDMED to identify adherence to the Mediterranean diet and associations with biological outcomes needs to be investigated in further studies. Finally, the cross-sectional design of the study and the lack of biological indicators did not allow us to estimate any causal relationship between lifestyle parameters and disease or potential health benefits from adherence to this specific dietary pattern. Although we attempted to account for variables that were possibly associated with KIDMED, the potential for residual confounding by uncontrolled covariates may be possible. 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