DETAILS OF AFFYMETRIX PROMOTION – HUMAN TRANSCRIPTOME (HTA) ARRAYS AND REAGENTS (2013) Sample Isolated from Fresh or Frozen Tissue: (Utilizes WT reagents) Out the door price [includes sample processing, reagents & array] = $390 / sample Promotion expires Friday Dec 27: Price will then increase to $500 / sample Sample Isolated from FFPE Material: (Utilizes Sensation Plus reagents) Out the door price [includes sample processing, reagents & array] = $490 / sample Promotion expires Friday Dec 27: Price will then increase to $600 / sample NOTE: Additional processing discounts are available for larger studies. Please inquire. Products HTA arrays only HTA arrays only HTA arrays WT+ reagents HTA arrays WT+ reagents HTA arrays and Sensation Plus reagents HTA arrays and Sensation Plus reagents Size Price Package/Bun Promo Pricing dled Pricing Good until Dec 27, 2013 2 10 10 30 $299 $299 $350 $350 $598 $2,990 $3,500 $10,500 12 $400 $4,800 24 $400 $9,592 $2,500 $7,500 $3,900 $7,800 If you wish to avail yourself of this promotion, contact Debora Poruban at 368-8748 or [email protected] by close of day Friday Dec 27th. Questions about the technology: Contact Marty Veigl [368-5689; [email protected]] or Patrick Leahy [368-0761; [email protected]]. Affymetrix Year-End Promotion The GEGF would like to let you know about an attractive promotion that Affymetrix is offering on their new and very exciting Human Transcriptome Array (HTA). This array which assesses total gene expression and alternative splicing transcripts, is an extremely competitive, budget-friendly alternative platform to RNA sequencing. The technology is capable of handling RNA from Fresh, Frozen and FFPE materials and works with starting amounts as little as 50ng of totRNA. See slide below for more details and a comparison of RNAseq and the HTA microarray platform. You can also visit the Affymetrix WebSite [http://www.affymetrix.com/catalog/prod760002/AFFY/Human-Transcriptome-Array-2.0#1_1] for more information on the HTA Expression Product. DETAILS OF AFFYMETRIX PROMOTION Sample Isolated from Fresh or Frozen Tissue: Out the door price [includes sample processing, reagents & array] = $390 / sample Promotion expires Friday Dec 27: Price will then increase to $500 / sample Sample Isolated from FFPE Material: Out the door price [includes sample processing, reagents & array] = $490 / sample Promotion expires Friday Dec 27: Price will then increase to $600 / sample NOTE: Additional processing discounts are available for larger studies. Please inquire. If you wish to avail yourself of this promotion, contact Debora Poruban at 368-8748 or [email protected] by close of day Friday Dec 27th. Questions about the technology: Contact Marty Veigl [368-5689; [email protected]] or Patrick Leahy [368-0761; [email protected]]. Alternate Splicing of gene transcripts is emerging as a critical factor in understanding disease pathologies. Compare Affy HTA and RNA sequencing approaches to alternative splicing Relative Cost per Sample HTA array RNAseq Cost/sample varies depending on size of target & depth of coverage. Coverage of Splice Junctions* HTA array *Nature Genetics 2008. Deep surveying of alternate splicing complexity in the human transcriptome by high throughput sequencing. Required 200X Coverage. Pan et al. RNAseq 24-48 samples per week Time To Results HTA array Affy tunable proprietary algorithms make alt. splice event calls automatic RNAseq What is on the HTA? 914,585 Total expression entities interrogated, made up of : 809,439 coding exons [10 probes detect each exon] 103,615 non-coding entities (lncRNAs, snoRNAs, tRNAs, miRNAs etc) 339,146 Known Splice junctions [ ~80-85% of all splice junctions]; 4 probes detect each splice junction)