H5: Complete molecular workflow - sample to results with real time PCR & pyrosequencing Date: - 15th October, Venue: - SMS Medical College, Jaipur Supported By: - Qiagen Organizing Coordinator: Dr. Deepthi Nair Faculty as Panelists: Dr. Deepthi Nair (Coordinator Panel discussion); Dr. Poonam Lumba (Prof, GB Pant hospital, Delhi); Dr. Malini R Capoor (Assistant Prof, VMMC & Safdarjung hospital, Delhi); Dr. Navin Kumar (Senior Consutant, Medanta, Gurgaon); Dr. Saritha Mohapatra (Assistant Prof. AIIMS, Delhi) Faculty Practical sessions: Dr. Kanchan (PD Hinduja hospital, Mumbai); Ms. Pratibha Sharma Local Facilitators Dr. Nazneen Pathan Dr. Sakshee Gupta Dr. Shiv Kumar Dr. Pallavi Goyal Dr. PVJ Reddy Dr. Pratibha Sharma Dr. Farah Deeba Dr. Khushbu Trivedi 9982144921 9929182512 8947937841 9314060616 8955683989 9413418920 8824724001 7665601410 Summary and concept: The diagnosis of infectious diseases has been revolutionized by the development of molecular techniques, foremost with the applications of amplification methods. Polymerase Chain Reaction (PCR) being the forerunner. Originally PCR evolved as a manual method which needed repeated standardization. Real time PCR a fully automated closed PCR system now allows rapid, easy, standardized testing with several clinical applications in infectious diseases which are approved for diagnostic use by various authorizing agencies as FDA, WHO, CE etc. The achievable high sensitivity and ease with which the method can be used to detect any known genetic sequence have led to its wide application in the microbiology. Real-time PCR assays have provided additional major contributions over conventional PCR, the ability to confirm the amplification product and to quantitate the target concentration. The products of amplification generated are usually confirmed using specific oligonucleotides or probes. However signature sequences of larger segments may not always be accurate as amplification methods are rife with errors both internal and external. Sequencing as a technique is the ‘gold standard’ for detecting the exact occurrence of the base pairs in the nucleotide sequences of the segment of DNA of interest also known as the target. The classical methods of sequencing have also been very laborintensive, extremely expensive and demanding in terms of infrastructure. The Nex-Gen sequencing methods have provided a base to allow this useful test to be applied at the clinical microbiology laboratory. Pyrosequencing is one such Nex-Gen sequencing method which has found widespread application especially in mycobacteriology, virology, bacteriology etc. Sequencing gives additional information up to genome level by which microbial genotyping including resistance typing can be mapped. Most of molecular techniques start with the isolation of pure Nucleic Acid and then downstream applications. Nucleic Acid extraction, Real Time PCR and Sequencing has major application in clinical microbiology. Objectives & Expected Outcome: The workshop is a combination of hands-on experiments; demonstrations and panel discussion on • Nucleic Acid isolation by manual method • Quality assessment of Nucleic Acid • PCR/Real time PCR, data analysis • Pyrosequencing hands on and application in Clinical Microbiology • Panel discussion on Relevance of Molecular tests in the ‘Real Time’ situation in a clinical Microbiology laboratory Participants will learn about different techniques used for Nucleic Acid isolation and the basics of Real Time PCR with emphasis on molecular workflow optimization and automation. They would get hands on experience on nucleic acid isolation by manual method, Real Time PCR based diagnostic assay & analysis on Real Time PCR system, hands on Pyrosequencing and its application in Clinical Microbiology This workshop on Molecular workflow solutions would help to learn some of the aspects of basic and advanced molecular techniques used in clinical microbiology. Scientific Program H5 Note :- Participants will be divided in two groups and will swipe groups after lunch Time 08.30 AM - 09.00 AM 09.00 AM - 11.00 AM 11.00 AM - 11.30 AM 11.30 AM - 01.00 PM 01.00 PM - 02.00 PM 02.00 PM - 04.00 PM 04.00 PM - 04.30 PM 04.30 PM - 05.30 PM Topics Introducation, Registration and Handing over of Pre-training assessment Forms to participants Nucleic acid isolation hands on session Tea - Coffee Break Real time PCR hands on session Lunch Break Pyrosequencing basics and Applications theoretical session ; Pyrosequencing hands on Tea - Coffee Break Panel discussion with experts and post workshop assessment Registered Delegates Sr. No. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 Registration No. 334 333 95 132 31 660 537 242 658 668 587 25 508 684 312 676 337 696 68 707 950 Name workshop Code Sourav Maiti H5+C1 Sana Ali H5+C1 Katara Neela H5 Deepashree R H5+C1 Chaudhary Anish H5 Piyali Haldar H5 Moytrey Chatterjee H5+C1 Tyagi Shivani H5+C1 Krishna Agarwal H5 Nilma Nuruddin Hirani H5+C1 Sanjay Kumar Biswas H5+C1 Rahul Lunagaria H5+C1 Bibekananda Panda H5+C1 Baliarsingh Madhusudan H5 Srinidhi B V H5+C1 Chaitra Shankar H5+C1 Anubha Patel H5+C1 Chakravorty Sriparna H5 Soni Priyanka H5+C1 Mukesh Kumar H5 Ruchika Bagga H5+C1