Rapid Glycoprotein Sample Preparation Platform Analysis What is GlykoPrep? GlykoPrep is a rapid, automatable sample preparation platform for glycoanalysis, which includes purification, deglycosylation, labeling and cleanup ready for analysis by a number of instrument systems. This unique platform streamlines workflows to reduce sample prep time to less than 3 hours. The GlykoPrep platform is composed of 4 modules: Purification Module Purify monoclonal antibodies from Cell Culture Supernatants/Crude Samples Digestion Module Optimized on-cartridge digestion reduces incubation times significantly Labeling Module Proprietary chemistry non-specifically labels released glycans instantly Integrates into any workflow, regardless of type of analysis being performed. The platform is modular, and you choose only what you need. Samples Useful for a variety of glycoprotein samples, including crude cell-culture supernatant and Fc-fusion proteins. Full Range of Automation GlykoPrep offers the full range of automation options, from manual to full, walkaway processing. Whether you do 5 or 5,000 samples a week, GlykoPrep accommodates your throughput needs. Quantitative Glycan analysis is no longer just qualitative, glycosylation can be determined quantitatively. Cleanup Module Cleanup free dye and elute in low volumes with no need to dry down ProZyme® and GlykoPrep™ are trademarks of ProZyme, Inc., Hayward, CA USA AssayMAP® is a trademark of BioSystem Development, LLC, Madison, WI USA. AssayMAP is a patent-pending technology of BioSystem Development Eppendorf ® is a trademark of Eppendorf, AG, Hamburg Germany Getting Started Heater and Incubation Blocks Necessary Equipment The GlykoPrep™ platform requires the following laboratory equipment: Getting Started Centrifuge and Rotor Heater and Incubation Blocks Necessary A centrifuge, capable of 50 - 1000 x g,Equipment and GS150 AssayMAP® Heater and Incubation Blocks microplate rotor with a height capacity ofplatform requires the The GlykoPrep™ Additionally, the AssayMAP® Dry Blockheater 44 mm is needed to pushfollowing mobile phase andequipment: laboratory and Incubation blocks (GS150) are needed for sample through the cartridges. Centrifuge and Rotor the Digestion Module of GlykoPrep. A centrifuge, capable of 50 - 1000 x g, and GS150 AssayMAP® Heater and Incubation Block Labware microplate rotor with a height capacity of Additionally, the AssayMAP® Dry Blockhe 44 mm is needed to push mobile phase and and(AM200) Incubationisblocks (GS150) are needed A Starter Labware Kit needed sample through the cartridges. the Digestion Module of GlykoPrep. to run a full 96 samples with GlykoPrep. Labware A Starter Labware Kit (AM200) is needed to run a full 96 samples with GlykoPrep. Eppendorf® 5804 Centrifuge AssayMAP® AM200 Starter Labware Kit OtherReagents/Equipment Needed Eppendorf® 5804 Centrifuge AssayMAP® AM200 Starter Labware Kit • Acetonitrile OtherReagents/Equipment Need • Multichannel Pipettor • P5 Pipettor (or Equivalent) • Acetonitrile • Multichannel Pipettor • P5 Pipettor (or Equivalent) Eppendorf® A-2-DWP (5804/10) with more than 44mm of height capacity Eppendorf® A-2-DWP (5804/10) with more than 44mm of height capacity 3832 Bay Center Place Hayward, CA 94545-3619 3832 Bay Center Place USA & Canada Hayward, CA 94545-3619 510-638-6900 (p) USA & Canada 510-638-6919 (f) 510-638-6900 (p) [email protected] 510-638-6919 (f) [email protected] Europa Bioproducts Ltd 15-17 North Street, Wicken Ely, Cambridgeshire CB7 5XW www.europa-bioproducts.com +44 (0)1353 721118