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Your Distributor:
Scientific-Instruments
Bucher Weg 14
D-73529 Schwäb. Gmünd
Tel: 07173/9155-99
Fax: 07173/9155-88
www.scientific-instruments.de
[email protected]
Measurement for Fluorescence Spectra of
Solid Sample
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
Fluorescence Spectra of Solid Samples
Exciting Light
Fluorescence
Solid Sample Holder
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
Fluorescence Spectra of Plate Samples
Glass
panel
Blue
Red
Green
Plasma Discharge
UV
Dielectric
Layer
UV
UV
UV
Plasma
Cell
Plasma Display
Measurement of Fluorescence Spectrum of PDP panel
- Development of plasma cell
- Quality control of display panel
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
3 colors
Fluorescence Spectra of Plate Samples
High Speed scanning and shorter time measurement
Blue
Exciting
Blue
Green
Red
Fluorescence
Blue
Green
3D Fluorescence Spectrum of PDP Display
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
Red
Fluorescence of White LED (Powder)
White LED
Blue Light（LED）
Intensity
Primary 3 Color
White Light
Yellow Light（FL）
Blue + Yellow
380
WL（nm）
Spectra of White LED
Yellow phosphor
：Convert Blue light to Yellow
Blue LED
：Blue lighting
Measurement of Fluorescence and Exciting spectra for Phosphor evaluation
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
780
Fluorescence of White LED (Powder)
Measurement of the Phosphors
YAG for white LED
Yellow Light
Intensity
Intensity
High Intensity with Blue Light
Sample Holder for Powder
WL(nm)
WL(nm)
Exciting and Fluorescence spectra of YAG (Powder)
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
Accurate Measurement of Spectra
Accessory:
- Filter Set
- Spectra Correction Kit
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
Effect of Cut Filter
No Cut Filter
Scattering
Light
Exciting Light
2nd Light
Scattering
Fluorescence
Na Salicylate Acid (Powder)
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
Cut Filter:
Pass Fluorescence and cut scattering light
Effect of Cut Filter
Filter (UV35）
Scattering
Light
Exciting Light
Scattering
Fluorescence
Na Salicylate Acid (Powder)
Cut Filter:
Pass Fluorescence and cut scattering light
Cut the scattering light and measure the samples with less effect of 2nd light
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
Effect of Cut Filter
No Filter
Scattering
2nd Light
Exciting Light
Filter (UV35）
Scattering
Fluorescence
Cut Filter:
Pass Fluorescence and cut scattering light
Cut the scattering light and measure the samples with less effect of 2nd light
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
Spectrum correction
Xe lamp and Detector have own characteristic
and spectrum of Fluorescence spectrophotometer
is effected on these factors.
Spectrum correction is necessary to get the
real spectrum
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
Spectrum correction
Na Salicylate Acid (No correction)
Na Salicylate Acid (Correction)
Spectrum
Correction
3D Spectrum without correction
3D Spectrum with Correction
Characteristics of light source, optical components and detectors can be corrected
and real spectrum can be measured.
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
Spectrum correction
Measurement of FL Standard Material
（NIST SRM 936a Quinine Sulfate）
FL Intensity
(Relative)
1.0
Measured Results
0
350
Certification
WL（nm）
600
Measurement results of NIST SRM 936a (1μg/mL)
It is possible to compare to other instruments, because of spectrum correction.
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
Measurement of luminescence spectrum
Measurement in Luminescence mode
（It’s necessary to shut the exciting light)
This is the custom order accessory
Large samples that cannot fit into
sample compartment can be measured.
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
Measurement of Optical Fiber Accessory
↑Sample measurement
Attachment
Fluorescence
< Future >
- Nondestructive measurement
- Measurable of Large sample
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
Exciting Light
WL: Ex, Em 300nm-750nm
(Depends on Transmittance of Optical Fiber)
Measurement of Optical Fiber Accessory
3 primary colors of light
100
FL intensity (a.u.)
Red
Blue
Green
0
400
wavelength
Luminescence spectrum of CRT monitor
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
750 nm
Measurement of FL distributions
Measurement with Micro Plate Accessory
- Set solid samples into each wells of micro plate
- 96 samples (8×12 wells) measurement：Size of Micro Plate
8 wells
12 wells
F-7000 with Micro Plate
Micro Plate Accessory can be used as Auto sample for Solid samples measurement
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
Measurement using Micro Plate Accessory
90.0-100.0
80.0-90.0
70.0-80.0
60.0-70.0
50.0-60.0
40.0-50.0
30.0-40.0
20.0-30.0
1
2
3
4
5
6
7
8
9
10
11
12
Beam Size: φ5mm
“FL” is written on the paper with Highlight marker pen
and measured the distribution of Fluorescence.
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
Measurement of Quantum Yield
of Solid Samples
Accessories for Q.Y Measurement:
- Φ60mm Integrating sphere
- Spectrum correction kit
- Quantum Yield Calculation Software
etc
Exciting
Fluorescence
Sample
Quantum yield is the rate of “Fluorescent amount” and “Absorbed amount”
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
What is the Quantum Yield ..
absorbance
1000
Irradiation with excitation light
（Energy）
Quantum yieldΦ=
Fluorescence
500
Quantum yield=0.50
Number of photons emitted
by the sample (fluorescent amount）
Number of photons absorbed
by the sample (absorbed amount)
<Purpose of Measurement>
- Efficiency to convert the absorbed light to fluorescence, index of the fluorescent material
- The higher the quantum yield, the better the quality of the fluorescent material
- Development and evaluation of new fluorescent probes for bio-industry
- Development and evaluation of organic EL materials and fluorescent substances for
lightening equipment
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
Introduction of F-7000 Accessories
Quantum Yield Units Accessory
Lighting, AV equipment, Film,
Filter and FL material
Quantum Yield Units:
60 integrating sphere, White Plate, Spectralon TM standard white plate,
Sample cell, Aluminum oxide powder, Computer Program, Manual
<Feature of F-7000 Quantum Yield System>
- High Sensitivity, Highly Accurate Measurement
with 6 Orders Wide Dynamic Range
- High Throughput Measurement by High-Speed Scan
- Measurement Over a Wide Wavelength Range of 240 to 800nm*1
- Large Selection of Calculation Items, Easy Operation by Special Software
*1: Optional Detector necessary
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
Measurement of Fluorescent Substance for Lamp
Measurement of MgWO4
Absorption Region
Fluorescence Region
Relative Fluorescence Intensity
Al2O3
MgWO4
Quantum Yield
Measurement Result
Quantum Yield
Reported Value 4)
⊿φ
: 0.80
: 0.81
: 0.01
Measurement Conditions
Excitation WL: 254nm
Measurement WL: 240-750nm
Calculation Condition
Scattered light range: 244-264nm
Fluorescence range: 350-750nm
Wavelength （nm）
Wavelength（nm）
Quantum Yield Measurement of MgWO4 (Powder)
All Rights Reserved, Copyright © 2008, Hitachi HighHigh-Technologies Corporation
4) J.Illum.Engng. Inst JPN. Vol.83 No2 1999