The benchmark in sample management™ BioProcessing Solutions BioStorage Technologies, Inc., the leading global provider of comprehensive sample management solutions, and RUCDR Infinite Biologics, a global leader in the bioprocessing and biobanking of samples for genetic, gene and cell-based research, have developed a strategic and innovative Bioprocessing Solutions Alliance which provides to the bioscience industry an integrated, state-of-the-art scientific approach and technology infrastructure for the delivery of advanced sample bioprocessing and biobanking solutions. We offer a full portfolio of translational research services: Sample Preparation and Extraction Services • Automated sample aliquoting and blood fractionation • Normalized biomaterial aliquoting/distribution • Tissue preparation and storage • DNA, RNA and miRNA extraction Cell Culture and Stem Cell Services • Cell line establishment and processing • Tissue biopsies • Lymphoblastoid cell line creation • Stem cell line derivation and processing Genetic and Genomic Analytical Services • Gene expression profiling (low, medium, high-throughput) • Genotyping (low, medium, high-throughput) • Next generation sequencing Bioinformatics/Analysis Services • Experimental design consulting • Data formatting and transfer • Analytical services We create renewable sample resources The Bioprocessing Solutions Alliance delivers to bioscience companies a comprehensive and global solution for the bioprocessing and storage of biological samples, transforming stored samples into renewable resources for future research. Blood Biofluids Whole Blood Plasma Saliva PAXgeneTM Preserved Whole Blood Serum Feces Buffy Coat Blood Spot Cards Urine Tissue Buccal Swabs Fresh Tissue Frozen Tissue PAXgeneTM Preserved Tissue FACS / LCM sorted cells FFPE We deliver innovative technology solutions Bioinformatics systems are fundamental to optimizing the value of biological sample collections to support research. The Bioprocessing Solutions Alliance offers integrated, state-of-the-art technologies to support the processing, tracking and analysis of research samples. ISISS® BioStorage Technologies provides a proprietary, industry-leading Intelligent Specimen Inventory Storage System (ISISS) which enables US FDA 21 CFR Part 11 compliant sample audit trail tracking and reporting of samples in storage. ISISS provides 24/7 real-time access to sample inventory data within seconds from anywhere in the world. RUCDR LIMS RUCDR Infinite Biologics provides a laboratory information management system (LIMS) which tracks the lifecycle of the sample as it moves through each stage of a service workflow within the laboratory. RUCDR LIMS automatically updates sample information within ISISS to provide a fully integrated individual sample tracking system across all bioprocessing services. We provide flexible sample storage solutions BioStorage Technologies, a world leader in comprehensive sample management, in partnership with RUCDR, the world’s largest universitybased biorepository, offers bestin-class sample storage services to support drug discovery and development including: Our integrated, consistent and comprehensive approach to sample processing and storage ensures sample viability for future research and analyses. Mechanical storage -70⁰C, -80°C, -20⁰C, 5°C Automated storage -80⁰C Liquid Nitrogen -190°C (liquid and vapor phase) Bulk or Non-Bulk 15⁰C - 27°C Sample preparation services lymphocytes from fresh blood to • Automated sample aliquoting utilizing liquid handling technologies produce lymphoblastoid cell lines assessment and primary analysis (LCLs), as well as transformation of of raw data. Downstream analyses cryopreserved lymphocytes isolated - Superior air displacement pipetting technology • Normalized biomaterial aliquoting/distribution • Isolation and cryopreservation of primary source cells from tissue and blood Cell culture and stem cell services The preparation, tracking, and - Customized sample preparation for analysis based on volume, concentration or quantity of nucleic acid in any user-defined vessel format processing of blood, tissue and • Tissue preparation and storage and banking of primary source cells various other sample types to - Micro/macro dissection and slide preparation for histological analyses, a variety of tissue preservation chemistries, FFPE processing, tissue sectioning, tissue curls, snap freezing, and tissue microarray generation from postnatal through adult human support laboratory and diagnostic analyses are offered through a variety of semi- and fully automated processing methods tailored to sample type, collection vessels and project criteria. Nucleic acid extraction services • DNA, RNA and miRNA extraction - DNA extraction capabilities consisting of organic, solid-phase, salting out and beadbased extractions chemistries from virtually any tissue source. Extracted RNA collected and stored from frozen tissue biopsies, FFPE block/slides, Paxgene RNA tubes, PaxTissue containers, RNAlater and Genotek Oragene RNA collection tubes in depth interpretation of complex Cell culture - Software allows for the customization of protocols - Fully automated blood fractionation that results in plasma/serum isolation, buffy coat isolation and red cell pellet isolation, all with aliquot number and volumes as protocol variables produce richly annotated reports for genomics datasets. - Configured for both 8- and 96channel pipetting • Automated blood fractionation platforms and enable rapid quality PCR/qPCR from blood. - In-line tube and 2D tube rack barcoding for replicate plating and high volume assay preparation Sample preparation and extraction services Nucleic acid amplification Our capabilities range from isolation subject tissue to derivation of new iPSC lines, including comprehensive validation and banking of iPSCs or similar reprogrammed/dedifferentiated cells. Capabilities also include transformation of • Lymphocyte isolation from whole blood for cryopreservation or transformation (immortalization) Stem cell services • Reprogramming of primary fibroblast, blood or lymphoblast cells to induced Pluripotent Stem Cells (iPSCs) using footprint-free derivation protocols and the establishment of fibroblast cell lines from tissue • Extensive genotypic and phenotypic characterization of primary, transformed and iPSC lines, including genomic integrity (karyotyping and array CGH), marker expression (FACS), pluripotency and differentiation assays • Experimental design consultation Sequencing Genetic and genomic analytical services Genotyping • Production of common genetic maps Bioinformatics and analytical services Gene Expression Multiple solutions for the robust A broad range of bioinformatics amplification of precious nucleic services are available for the analysis acid samples, as well as restoration and visualization of genomic data. of degraded or compromised Microarray Sophisticated software packages material. Whole-genome and accept output files from microarray whole-transcriptome amplification and next-generation sequencing chemistries enable highly uniform and unbiased enrichment, preserving relative abundance and Molecular Genetics Diagnostic Services allelic balance for downstream genetic analyses. Assay Design • Resolution of linkages or genotyping discrepancies • DNA identity fingerprinting • Functional data analysis for genome and transcriptome wide analyses • Customizable data formatting “Our mission is to develop the most innovative processes and technologies which enable research organizations to identify and maintain the most valuable biological samples to support the development of future biomarkers, diagnostics and drug therapies.” Dr. Andrew Brooks, COO, RUCDR Infinite Biologics BioStorage Technologies Headquarters 2910 Fortune Circle West, Suite E Indianapolis, IN 46241 Phone: +1 866 697 2675 Fax: +1 317 390 1868 BioStorage Technologies European Operation Im Leuschnerpark 1B 64347 Griesheim Germany Phone: +49 6155 898 1011 Fax: +49 6155 898 1090 BioStorage Technologies Asia Pacific Operation 4 Kaki Bukit Ave 1, #07-04, Singapore 417939 Phone: +65 (3) 1576199 Suite 2401, Room 2480 China Central Place, Tower 3 77 Jianguo Road, Chaoyang District Beijing 100025, China Phone: +86 10 8587 2390 Fax: +86 10 8588 0220 www. biostorage.com The benchmark in sample management™ RUCDR Infinite Biologics 604 Allison Road Piscataway, NJ 08854-8009 Phone: +1 732 445 1498 www. rucdr.org