Sample Handling SPE & Filtration .. .. .. .. .. .. .. .. .. .. .. .. .. .. ..1-39 SPE Introduction .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 2-5 Drug-Clean™ SPE .. .. .. .. .. .. .. .. .. .. .. .. .. 6-7 Ultra-Clean™ SPE .. .. .. .. .. .. .. .. .. .. .. .. .. .. 8 Maxi-Clean™ SPE .. .. .. .. .. .. .. .. .. .. .. .. .. .. 9 Reversed-Phase SPE .. .. .. .. .. .. .. .. .. .. 10-13 Normal-Phase SPE .. .. .. .. .. .. .. .. .. .. .. 14-16 Specialty SPE .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 17 Ion Exchange & IC SPE .. .. .. .. .. .. .. .. .. 18-21 SPE Accessories .. .. .. .. .. .. .. .. .. .. .. .. 22-26 Filtration Introduction .. .. .. .. .. .. .. .. .. .. .. 27-29 Syringe Filters .. .. .. .. .. .. .. .. .. .. .. .. .. .. 30-35 Other Filters & Accessories .. .. .. .. .. .. .. 36-39 Standards .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 40-54 Derivatization Reagents .. .. .. .. .. .. .. .. .. .. 40-44 Ion-Pair Reagents .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 45 Gas Standards .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 46-48 Drug Standards .. .. .. .. .. .. .. .. .. .. .. .. .. .. 49-52 FAME & Hydrocarbon Standards .. .. .. .. .. 53-54 Syringes .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 55-71 Introduction .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 55 Hamilton Syringes .. .. .. .. .. .. .. .. .. .. .. .. .. 56-64 Popper Syringes .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 65 VICI-Precision Syringes .. .. .. .. .. .. .. .. .. .. 66-67 SGE Syringes .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 68-70 Pipetors & Dispensers .. .. .. .. .. .. .. .. .. .. .. .. .. 71 Vials .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 72-122 Introduction .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 72-77 12 x 32mm Vials .. .. .. .. .. .. .. .. .. .. .. .. .. .. 78-92 8 x 40mm Vials .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 93 15 x 45mm Vials .. .. .. .. .. .. .. .. .. .. .. .. .. .. 94-97 Specialty Vials .. .. .. .. .. .. .. .. .. .. .. .. .. .. 98-103 Storage Vials, EPA Vials .. .. .. .. .. .. .. .. ..104-113 96-Well Products .. .. .. .. .. .. .. .. .. .. .. .. ..114-117 © Copyright 2004 Alltech Associates, Inc. Accessories .. .. .. .. .. .. .. .. .. .. .. .. .. .. ..118-121 Vial Crusher .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. 122 Contact your Alltech office or distributor for current or local prices. 1 SPE SPE Introduction Choosing an SPE Product 1. Characterize the sample. Factors such as the analyte’s polarity relative to the matrix, the presence of charged functional groups, solubility, molecular weight, etc., determine how strongly the analyte is retained by the packed bed. 2. Select a retention strategy. There are two basic methods for sample treatment: a. Select the packing bed to retain the desired analyte. The contaminants are washed off and the desired analyte is then eluted for analysis. b. Select the packing bed to retain the contaminants and the desired analyte passes directly through. 4914 3. Select the proper packing type for the cleanest extract with the highest recovery. a. Reversed-phase packings are hydrophobic, silica-based materials that retain moderately polar to nonpolar compounds from a polar matrix while washing off polar interferences. Or you can retain non-polar contaminants while the polar compounds pass through unretained. b. Normal-phase packings are hydrophilic, silica-based materials that retain polar compounds from a non-polar matrix while washing off non-polar interferences. Or you can retain polar contaminants while non-polar compounds pass through unretained. c. Ion-exchange resins retain charged compounds or remove ionic interferences. 4. Optimize conditions for best results. Select proper bed size and suitable conditioning, wash and elution solvents. a. Poor sample recovery often occurs when the packed bed dimensions are not optimized. Too large a bed results in incomplete elution while too small a bed results in incomplete retention. b. Consider the solvent strength relative to the packing material. The final conditioning solvent should be weak so it doesn’t act as an eluting solvent. Buffers should be used to control ionization of potentially charged compounds. c. Wash solvents should remove weakly retained interferences without being strong enough to elute the analyte. d. Elution solvents should be strong enough to completely elute an analyte in a small volume (1-2mL). For Environmental Applications Recommended SW-846 Cleanup Methods EPA ANALYTE GROUP METHOD NO. Aniline and Derivatives 3620 Chlorinated Pesticides 3620 Chlorinated Hydrocarbons 3620 Haloethers 3620 Nitroaromatics and Cyclic 3620 Ketones Nitrosamines 3610, 3620 Organochlorine Pesticides 3620 Organophosphorus Pesticides 3620 PCB’s 3620, 3630 Petroleum Waste 3611 Phenols 3630 Phthalate Esters 3610, 3620 Polynuclear Aromatic 3630 Hydrocarbons © Copyright 2004 Alltech Associates, Inc. 2 RECOMMENDED SPE SORBENTS C18 C18, Florisil® C18, Phenyl C18, Florisil® DVB, IC-RP Need Technical Assistance? Call Alltech Tech Support: Email: [email protected] Online: www.alltechWEB.com C18 C18, Florisil® Florisil®, Carbograph C18, SCX, Carbograph Alumina C18, Phenyl C18, DVB C18, Phenyl Contact your Alltech office or distributor for current or local prices. SPE SPE Introduction SPE Sorbent Specifications SPE Sorbent Specifications FUNCTIONAL GROUP BASE % CARBON ENDCAPPED Reversed-Phases (Non-Polar) Prevail C18 Silica 11.0% Yes Octadecyl (C18) Silica 6.0% Yes High Flow C18 Silica 8.0% Yes High Capacity C18 Silica 17.0% Yes Large Pore C18 Silica 14.0% Yes Oil & Grease C18 Silica 17.0% Yes Octyl (C8) Silica 4.5% Yes High Capacity C8 Silica 8.5% Yes Ethyl (C2) Silica 5.5% Yes Phenyl (PH) Silica 3.8% Yes Cyclohexyl (CH) Silica 3.5% Yes Normal-Phases (Polar) Silica (SI) Silica — — Aminopropyl (NH2) Silica 5.0% No Cyanopropyl (CN) Silica 6.0% Yes Diol (2OH) Silica 4.0% No Florisil® (FL) (FL-PR) Magnesium Silicate — — Alumina (AL) Aluminum Oxide — — Specialty Packings DVB 100% DVB — — Carbograph Graphitized Carbon — — Drug-Clean™ Silica — — Drug-Clean™ PB Polymer — — AVERAGE PARTICLE SIZE PORE SIZE 50µm 50µm 100µm 50µm 50µm 100µm 50µm 50µm 50µm 50µm 50µm 60Å 60Å 60Å 60Å 150Å 60Å 60Å 60Å 60Å 60Å 60Å PAGE How to Use Solid Phase Extraction Products 10 12 12 12 12 12 13 13 13 13 13 50µm 50µm 50µm 50µm 75-150µm 130µm 60Å 60Å 60Å 60Å 60Å 100Å 15 14 14 14 16 16 40µm 38-125µm 50µm 30µm — — 60Å — 17 17 6 7 By syringe 4915 Ion Exchange Specifications PACKING TYPE SCX SAX IC-OH IC-H IC-Ag IC-Ba IC-Na IC-Chelate IC-RP BASE Styrene-DVB Styrene-DVB Styrene-DVB Styrene-DVB Styrene-DVB Styrene-DVB Styrene-DVB Styrene-DVB Polystyrene COUNTER ION Hydrogen Acetate Hydroxide Hydronium Silver Barium Sodium Sodium — PARTICLE SIZE 35-70µm 35-70µm 35-70µm 35-70µm 35-70µm 35-70µm 35-70µm 35-70µm 250-850µm MOLECULAR EXCLUSION LIMIT 1000 Daltons 1000 Daltons 1000 Daltons 1000 Daltons 1000 Daltons 1000 Daltons 1000 Daltons 1000 Daltons — EXCHANGE CAPACITY 2.0meq/mL 1.0meq/mL 1.0meq/mL 2.0meq/mL 2.0meq/mL 2.0meq/mL 2.0meq/mL 0.4meq/mL — PAGE 18 18 20 20 20 20 20 20 20 By vacuum manifold 6275 Solvent Polarity Chart Less Polar (Stronger Reversed-Phase Elution) Hexane Isooctane Cyclohexane Carbon tetrachloride Toluene Chloroform Methylene chloride Tetrahydrofuran Ethyl ether Acetone Acetonitrile Pyridine Isopropanol Ethanol Methanol Water By filter flask 4919 Acetic acid More Polar (Stronger Normal-Phase Elution) © Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 3 SPE SPE Introduction SPE Column Formats Actual Size Drawings Extract-Clean™ and Ultra-Clean™ Columns 4976 1.5mL 4975 4974 8.0mL 4.0mL Extended Volume Columns 4973 15mL 4972 25mL Maxi-Clean™ Cartridges 4977 4978 4971 300mg Bed 600mg Bed 75mL 4979 900mg Bed 6276 10.0mL 100-500mg Bed Device Specifications DEVICE Extract-Clean™ Columns Ultra-Clean™ Columns Extended Volume Columns Maxi-Clean™ Cartridges 96-Well SPE Plates © Copyright 2004 Alltech Associates, Inc. 4 HOUSING Polypropylene Treated Polypropylene Polypropylene Polypropylene Polypropylene FRIT MATERIAL 20µm Polyethylene 10µm PTFE 20µm Polyethylene 20µm Polyethylene 20µm Polyethylene DESCRIPTION For a Full Range of Sample Sizes Inert Format for Sensitive Analyses 10mL Flared Design for Dilute Samples Luer-Inlet Cartridges for Manual Processing 2.0mL Square Well for Automated Procedures Contact your Alltech office or distributor for current or local prices. SPE SPE Introduction General Solid-Phase Extraction Procedures: Extract-Clean™ Method Development Kits Reversed-Phase Method Development Kits include a variety of packing materials to rapidly identify the packing which provides the cleanest extract and highest recovery for your sample. To isolate relatively non-polar compounds from a polar matrix A. Conditioning Rinse device with 3-5mL of methanol followed by 3-5mL of deionized water/buffer (do not allow bed to dry before next step). B. Sample Application Apply sample to the top of the device and draw through the packing bed. Flows of 1-5mL/min are optimal. C. Wash Wash with 5mL of a polar solvent if analyte is to be retained. D. Elution Elute analyte into a collection tube with 1-5mL of a non-polar solvent. Choose a Reversed-Phase Kit to retain moderately polar to non-polar compounds from a polar matrix. Choose a Normal-Phase Kit to retain polar compounds from a non-polar matrix. Complete Kits are available with reversed-phases, normal-phases, and ion exchangers. Normal-Phase To isolate polar compounds from a non-polar matrix A. Conditioning Rinse device with 3-5mL of non-polar solvent. B. Sample Application Apply sample to the top of the device and draw through the packing bed (1-5mL/min). C. Wash Wash with 5mL of a non-polar solvent if analyte is to be retained. D. Elution Elute analyte into a collection tube with 1-5mL of a polar solvent. Ion-Exchange To isolate charged or potentially charged compounds A. Conditioning Rinse device with 3-5mL of deionized water or low ionic strength buffer (0.01M). B. Sample Application Apply sample to the top of the device and draw through the packing bed at 1mL/min (ion exchange kinetics are slower than reverse or normal-phase). C. Wash Wash with 5mL of deionized water or low ionic strength buffer. D. Elution Elute analyte into a collection tube with 1-5mL of buffer at high ionic strength (0.1-1M) or modified pH (pH such that analyte is uncharged). © Copyright 2004 Alltech Associates, Inc. 5493 METHOD DEVELOPMENT KITS BED WT, COLUMN SIZE PART NO. Normal-Phase Kits 100mg, 1.5mL (Includes 10 columns each of 211200 Silica, Florisil®, Amino, CN, and Diol) 500mg, 4.0mL (Includes 5 columns each of Silica, Florisil®, Amino, CN, and Diol) PRICE 211300 Reversed-Phase Kits 100mg, 1.5mL (Includes 10 columns each of C18, 211001 C8, C2, and Phenyl) 500mg, 4.0mL (Includes 5 columns each of C18, C8, C2, CH, and Phenyl) 211100 Complete Kits 100mg, 1.5mL (Includes 10 columns each of C18, 210100 C8, C2, Diol, CN, NH2, Silica, SAX, and SCX) 500mg, 4.0mL (Includes 5 columns each of C18, C8, C2, CH, Diol, CN, NH2, Silica, SAX, and SCX) 210500 SPE packings only function properly after wetting the packing surface. The conditioning step establishes a dynamic equilibrium between the solvent and the packing material. For reproducible results, do not allow the packing to dry between the conditioning step and addition of the sample. Contact your Alltech office or distributor for current or local prices. 5 Drug-Clean ™ SPE SPE Drug-Clean™ Mixed Mode SPE Columns For Accurate Drug Analysis Results • Efficient—higher recoveries with minimal impurities compared to single mode extractions • Multi-functional—extract acidic, neutral, and basic compounds with a single SPE bed • Reproducible—true copolymer is cleaner and more accurate than mixed beds Drug-Clean™ phases are true copolymeric packings with both reversed-phase and ion exchange groups. Drug-Clean™ C is a C8/SCX cation function. This is used to retain neutral or amine containing compounds while allowing carboxylate groups to pass through. 4886 6305 Mixed mode allows high drug recoveries with minimal impurities compared to single mode extractions Drug-Clean™ A is a C8/SAX anion function. In this case the neutral or carboxylate compounds are retained and the amine group retention is minimized. Each box of columns includes extraction methods for the most popular NIDA drugs of abuse. Additional methods are available on Alltech’s website. Cocaine and Benzoylecgonine Extract from Urine � Drug Clean™ Specifications C8/Ion Exchange Functional Group: 50µm irregular silica, 60Å Base: Retention Mechanism: Mixed mode, Reversed-Phase, and Ion Exchange DRUG-CLEAN™ COLUMNS BED WT./COLUMN SIZE QTY. PART NO. PRICE Drug-Clean™ C (Mixed Mode Cation) Columns 100mg/1.5mL 100/pk 207015 200mg/4.0mL 50/pk 207010 500mg/4.0mL 50/pk 207017 500mg/8.0mL 30/pk 207019 Drug-Clean™ C (Mixed Mode Cation) Extended Volume Columns 200mg/10.0mL EV 30/pk 207011 Drug-Clean™ A (Mixed Mode Anion) Columns 100mg/1.5mL 100/pk 207030 200mg/4.0mL 50/pk 207034 Drug-Clean™ A (Mixed Mode Anion) Extended Volume Columns 200mg/10.0mL EV 30/pk 207038 2656 1. Cocaine (2.5mg/mL) 2. Benzoylecgonine-TMS, Benzoylecgonine-d3-TMS (5.0mg/mL) � � � � � � � �� �� �� �� ������� Column: Heliflex® AT-5ms, 30m x 0.25mm x 0.25µm Capillary GC Column (Part No. 15807) Temp: 225ºC (0 min hold) to 315ºC (11 min hold) at 10ºC/min Carrier Gas: Helium at 0.8mL/min (28cm/sec) Detector: MSD at 325ºC Sample Prep: Drug-Clean™C Request Sheet M102 for more information about these Drug-Clean columns. Why is a “True Copolymer” Better Than a Mixed Bed? By definition, a mixed mode packing contains two different functional groups on the same sorbent. To minimize costs, two individual packings are sometimes blended together physically and packed in a tube. This gives the effect of a mixed mode but in fact is a mixed bed packing with batch-to-batch reproducibility dependent on the blending skills of the manufacturer. A true mixed mode has the functional groups polymerized on the same silica base. This copolymer treatment assures a more predictable packing performance which means more reproducible results from tube to tube, batch to batch. © Copyright 2004 Alltech Associates, Inc. 6 Contact your Alltech office or distributor for current or local prices. SPE Drug-Clean™ SPE Drug-Clean™ PB SPE Columns For Cleaner Extractions and More Accurate Drug Analysis • Stable from pH 1 to 14 • No conditioning steps required • Faster flow rates than silica-based materials Drug-Clean PB has a C8/SCX cation function on a highly cross-linked styrene divinylbenzene base. The high sorbent capacity greatly reduces the amount of packing required for separation and makes this material ideal for use in 96-well plates. Each box of columns includes extraction methods for amphetamines, opiates, cocaine/benzoylecgonine, phencyclidine and carboxy-THC. 4886 Extract acidic, neutral, and basic compounds from a single column. Amphetamines Extract from Urine Drug Clean™ PB Specifications C8/Cation Function Functional Group: 30µm Styrene/DVB Polymer Base: Retention Mechanism: Mixed mode, reversed-phase and ion exchange DRUG-CLEAN™ PB SPE COLUMNS BED WT./COLUMN SIZE QTY. PART NO. Extract-Clean™ Columns 30mg/1.5mL 100 250120 30mg/4.0mL 50 250130 50mg/8.0mL 50 250140 96-Well Plate 30mg per Well ea 250150 4888 2673 1 1. Amphetamine (2.5 mg/mL) 2. Methamphetamine (2.5 mg/mL) 2 PRICE 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 Min. Column: Temp: Carrier Gas: Detector: Heliflex® AT-35, 30m x 0.25mm x 0.25µm Capillary GC Column (Part No. 13642) 180°C to 300°C at 10°C/min Helium at 0.7mL/min (24cm/sec) FID at 275°C Cocaine and Benzoylecgonine Extract from Urine 2 See pages 114-117 for collection plates and other 96 well accessory products. 2674 1. Cocaine (2.5 mg/mL) 2. Benzoylecgonine (5.0 mg/mL) 1 Solid-Phase Extraction Principles and Practice This excellent textbook covers SPE theory, method development, SPE materials and applications. 0 2 4 Column: SOLID-PHASE EXTRACTION PRINCIPLES AND PRACTICE DESCRIPTION PART NO. Solid-Phase Extraction Principles and Practice 1983 © Copyright 2004 Alltech Associates, Inc. PRICE Temp: Carrier Gas: Detector: 6 8 10 12 14 16 18 Min. Heliflex® AT-5ms, 30m x 0.25mm x 0.25µm Capillary GC Column (Part No. 15807) 225°C to 315°C at 10°C/min Helium at 0.7mL/min (25cm/sec) FID at 325°C Contact your Alltech office or distributor for current or local prices. 7 SPE Ultra-Clean SPE ™ Ultra-Clean™ SPE Ideal for Environmental, Pharmaceutical and Other Sensitive Applications • Treated columns with the inertness of PTFE • PTFE frits for low extractables • Unbreakable and less expensive than glass PACKING Reversed-Phase C18 C8 No Broken Glass! Normal-Phase Silica Florisil® Florisil®-PR Aminopropyl Carbograph 6197 Ion-Exchange SCX SAX ULTRA-CLEAN™ SPE COLUMNS BED COLUMN WEIGHT SIZE QTY. PART NO. PRICE 200mg 500mg 200mg 500mg 500mg 4.0mL 8.0mL 4.0mL 4.0mL 8.0mL 50 30 50 50 30 505150 505355 506151 506251 506351 200mg 500mg 5000mg 500mg 1000mg 500mg 1000mg 200mg 500mg 150mg 300mg 4.0mL 8.0mL 25mL 4.0mL 8.0mL 4.0mL 8.0mL 4.0mL 4.0mL 4.0mL 8.0mL 50 30 20 50 30 50 30 50 50 50 30 509150 509225 509010 507851 507930 504651 507900 511025 511150 510142 510101 200mg 500mg 200mg 500mg 4.0mL 4.0mL 4.0mL 4.0mL 50 50 50 50 509826 509951 509626 509751 See page 3 for packing specifications. Compare Ultra-Clean™ to Glass! 10025-27 Column: Alltima™ C18, 5µm, 150 x 4.6mm HPLC Column (Part No. 88052) Mobile Phase: A: Water B: Acetonitrile Gradient: Time: 0 2 20 %B: 5 5 70 Flow Rate: 1mL/min Detector: UV at 210 10028-30 Column: Alltima™ C18, 5µm, 150 x 4.6mm HPLC Column (Part No. 88052) Mobile Phase: A: Water B: Acetonitrile Gradient: Time: 0 2 20 %B: 5 5 70 Flow Rate: 1mL/min Detector: UV at 210 No new contaminants are introduced by Ultra-Clean™ SPE columns when compared to glass SPE columns. © Copyright 2004 Alltech Associates, Inc. 8 Contact your Alltech office or distributor for current or local prices. SPE Maxi-Clean™ Cartridges Maxi-Clean™ Cartridges The Flexible Cartridge Format Saves Time and Maximizes Your Sample Preparation Efficiency • Adaptable—Same bed dimensions as 4mL SPE columns for method cross-over; Process a single cartridge by syringe or multiple cartridges by vacuum • Versatile—Stack different cartridges for multistep extractions; Use top and bottom caps for easy transport of field samples MAXI-CLEAN™ REVERSED-PHASE CARTRIDGES PACKING BED WEIGHT QTY. PART NO. Prevail™ C18 300mg 50 605926 500mg 50 605929 900mg 50 605942 C18 300mg 25 20924 300mg 50 20926 300mg 100 20928 600mg 25 20932 600mg 50 20934 600mg 100 20936 900mg 25 20940 900mg 50 20942 900mg 100 20944 High Capacity C18 300mg 50 20945 Large Pore C18 300mg 100 22012 600mg 100 22017 900mg 100 220215 C8 300mg 50 20950 600mg 50 20958 900mg 50 20966 C2 300mg 50 210064 Phenyl 300mg 25 22002 300mg 50 22003 300mg 100 22004 MAXI-CLEAN™ DRYING CARTRIDGES PACKING BED WEIGHT QTY. PART NO. Sodium Sulfate 3g 100 219001 PRICE PRICE 4881 4915 MAXI-CLEAN™ NORMAL-PHASE CARTRIDGES PACKING BED WEIGHT QTY. PART NO. Silica 300mg 25 20972 300mg 50 20974 300mg 100 20976 600mg 25 20980 600mg 50 20982 600mg 100 20984 900mg 25 20988 900mg 50 20990 900mg 100 20992 Florisil® 300mg 50 210054 300mg 100 210056 900mg 50 210057 900mg 100 210061 Florisil®-PR 300mg 50 210074 300mg 100 210076 900mg 50 210079 900mg 100 210075 Alumina, Basic 300mg 25 210093 1800mg 25 210096 Alumina, Acidic 300mg 25 210094 1800mg 25 210097 Alumina, Neutral 300mg 25 210095 1800mg 25 210098 Diol 300mg 50 210084 300mg 100 210086 Cyanopropyl 300mg 50 210034 300mg 100 210036 Aminopropyl 300mg 50 210044 300mg 100 210046 900mg 100 210047 PRICE What is a Maxi-Clean™ Cartridge? A Maxi-Clean™ cartridge is an alternative format for SPE. It uses the same high quality packing materials as the Extract-Clean™ columns but has a polypropylene housing with both a female luer inlet and a male luer outlet tip. This allows use of positive pressure from a syringe or negative pressure from a vacuum manifold. 20µm polyethylene frits are placed at each end of the sorbent bed. The packing material is packed and compressed to improve or optimize flow characteristics. For ion-exchange SPE cartridges see pages 20-21. © Copyright 2004 Alltech Associates, Inc. For packing specifications see page 3. Contact your Alltech office or distributor for current or local prices. 9 SPE Reversed-Phase SPE Prevail™ SPE – A Universal C18 • Saves Time—Since it does not require preconditioning with an organic solvent, it eliminates one step from typical SPE procedures. • Saves Solvents—Eliminating organic preconditioning reduces handling and disposal of expensive, hazardous organic solvents. • Universal Applications—Prevail™ C18 for SPE is useful for the same compounds and sample types as a traditional C18 phase. Prevail™ SPE Specifications Functional Group: Base: Percent Carbon Loading: Retention Mechanism Standard C18 Shows No Retention Prevail™ C18 Shows Excellent Retention 6293 2mL Methylene Blue solution through dry, unconditioned beds Modified C18 50µm irregular silica, 60Å 11% Reversed-phase Dr. Peaks says, Prevail™ SPE Columns are ideal for: • Drug Metabolites from Blood • Hormones from Body Fluids • Nutraceuticals from Foods & Beverages • Pesticides from Water Extract-Clean™ SPE Columns • Larger bed weights • Process by vacuum 4886red BED WEIGHT 100mg 130mg 500mg 500mg 1000mg PREVAIL™ C18 EXTRACT-CLEAN™ COLUMNS COLUMN SIZE QTY. PART NO. 1.5mL 100 605001 4.0mL 50 605130 4.0mL 50 605250 8.0mL 30 605350 8.0mL 30 605430 Caffeine in Coffee PRICE � Unextracted 10136 1. Caffeine Column: Prevail™ C18, 5µm, 150 x 4.6mm HPLC Column (Part No. 99208) Mobile Phase: Water:Acetonitrile (70:30) Flow Rate: 1.0mL/min Detector: UV at 254 Maxi-Clean™ SPE Cartridges • Small and portable • Process by vacuum or syringe � � � � � ������ � Extracted 4882red PREVAIL™ C18 MAXI-CLEAN™ CARTRIDGES BED WEIGHT QTY. PART NO. 300mg 50 605926 500mg 50 605929 900mg 50 605942 � 10137-38 PRICE ������� ��������������� ������� � © Copyright 2004 Alltech Associates, Inc. 10 � � � � ������ Contact your Alltech office or distributor for current or local prices. SPE Reversed-Phase SPE C18 SPE Applications Hypericins in St. John’s Wort Hypericins from St. John’s Wort � Procedure Using Extract-Clean™ High Capacity C18, 1000mg: 9107 A. Sample Treatment Pulverize 300mg St. John’s Wort powder into 5, 3mL aliquots of 80/20 MeOH/H20. Combine and filter extracts to produce 15mL filtrate. Dilute to 30mL with water. 1. Pseudohypericin 2. Hypericin � B. Conditioning 1. Rinse device with 5mL methanol. 2. Rinse device with 5mL deionized water. C. Sample Application Apply 2mL filtrate to the top of the SPE device and draw through the C18 packed bed. D. Wash* Wash device with 3mL of deionized water. E. Elution Elute with 2mL methanol. *Repeat load and wash steps consecutively until filtrate is consumed. � � Column: Mobile Phase: Flow Rate: Detector: � ������ Alltima™ C18, 3µm 53 x 7mm Rocket™ HPLC Column MeOH:0.2% H3PO4 (95:5) (Part No. 50605) 4.0mL/min VIS at 585nm Phenolic Acids in Echinacea Phenolic Acids from Echinacea � Procedure Using Extract-Clean™ High Capacity C18, 1000mg: A. Sample Treatment Pulverize 300mg Echinacea powder into 5, 3mL aliquots of methanol. Combine and filter extracts to produce 15mL filtrate. Dilute to 30mL with water. 9110 1. Echinicoside 2. Chlorogenic Acid 3. Caffeic Acid � B. Conditioning 1. Rinse device with 5mL methanol. 2. Rinse device with 5mL deionized water. C. Sample Application Apply 2mL filtrate to the top of the SPE device and draw through the C18 packed bed. D. Wash* Wash device with 2mL of deionized water. E. Elution Elute with 5mL of 50:50 methanol/water. *Repeat load and wash steps consecutively until filtrate is consumed. � � Column: Mobile Phase: Flow Rate: Detector: A. Conditioning 1. Rinse device with 10mL of hexane and 10mL of ethyl acetate. Pass air through device to remove excess solvent. 2. Rinse device with 5mL of methanol and 5mL of deionized water. D. Elution Elute with 2mL of 70:30 hexane:ethyl acetate. Pass extract through 2g-3g sodium sulfate to remove residual water. © Copyright 2004 Alltech Associates, Inc. Alltima C18, 3µm 53 x 7mm Rocket™ HPLC Column ACN:10mM K2HPO4, pH 2.6 (20:80) (Part No. 50605) 3.5mL/min UV at 330nm Recovery (as % Standard) 1668 1. α−BHC (104%) 2. Lindane (109%) � � 3. β−BHC (106%) 7. Heptachlor Epoxide (107%) 4. Heptachlor (92%) 8. α-Endosulfan (105%) � 5. δ−BHC (110%) 9. p,p’-DDE (79%) � � � 6. Aldrin (98%) 10. Dieldrin (102%) �� �� 11. Endrin (104%) �� 12. p,p’-DDD (92%) �� 13. β-Endosulfan (103%) �� �� 14. p,p’-DDT �� 15. Endrin Aldehyde (102%) �� �� 16. Endosulfan Sulfate (103%) 17. Methoxychlor (99%) ������� 18. Endrin Ketone (104%) � �� Procedure Using Extract-Clean™ Hi-Flow C18, 500mg: C. Wash Wash device with 10mL of deionized water then 10mL of 20: 80 methanol:deionized water. Remove excess by passing air through the device for two minutes. ������ Chlorinated Pesticides, 1ppb Chlorinated Pesticides from Water B. Sample Application Pass 100mL - 500mL (containing 1% methanol) of water sample through the device at 20mL/minute. � ™ � � Column: Temp: Carrier Gas: Detector: AT™-Pesticide Capillary GC Column, 20m x 0.53mm x 0.60µm (Part No. 16846) 210°C Helium, 35cm/sec ECD Contact your Alltech office or distributor for current or local prices. 11 SPE Reversed-Phase SPE C18 • Choose for highly non-polar compounds C18 Extended Volume Columns C18 Specifications PARTICLE SIZE SORBENT Standard C18 50µm High Capacity C18 50µm High Flow C18 100µm 50µm Large Pore C18 Oil & Grease C18 100µm PORE CARBON SIZE LOAD 60Å 6% 60Å 17% 60Å 8% 150Å 14% 60Å 17% CHOOSE FOR: Optimized Recoveries Stronger Retention Viscous Samples Macromolecules Highly Viscous Samples; Compatible w/EPA 1664 C18 Extract-Clean™ and Ultra-Clean™ Columns 6305 BED WEIGHT 100mg 200mg 500mg C18 EXTENDED VOLUME COLUMNS COLUMN SIZE QTY. PART NO. 20mL 30 405001 18mL 30 405150 18mL 30 405250 C18 Maxi-Clean™ Cartridges 4882 4886 C18 COLUMNS BED WEIGHT COLUMN SIZE QTY. PART NO. C18 Extract-Clean™ Columns 50mg 1.5mL 100 204900 100mg 1.5mL 100 205000 200mg 4.0mL 50 205150 500mg 4.0mL 50 205250 500mg 8.0mL 30 205350 1000mg 8.0mL 30 205430 2000mg 8.0mL 30 205450 2000mg 15mL 30 205462 5000mg 25mL 20 225450 10,000mg 75mL 16 235410 High Capacity C18 Extract-Clean™ Columns 50mg 1.5mL 100 255050 100mg 1.5mL 100 255100 200mg 4.0mL 50 255200 500mg 4.0mL 50 255300 500mg 8.0mL 30 255350 1000mg 8.0mL 30 255430 2000mg 15mL 30 255440 5000mg 25mL 20 255450 10,000mg 75mL 16 255460 High Flow C18 Extract-Clean™ Columns 500mg 4.0mL 50 215250 1000mg 8.0mL 30 215430 Oil & Grease C18 Extract-Clean™ Columns 4000mg 25mL 20 202973 C18 Ultra-Clean™ Columns 200mg 4.0mL 50 505150 500mg 8.0mL 30 505355 © Copyright 2004 Alltech Associates, Inc. 12 PRICE C18 MAXI-CLEAN CARTRIDGES BED WEIGHT QTY. PART NO. C18 Maxi-Clean™ Cartridges 300mg 25 20924 300mg 50 20926 300mg 100 20928 600mg 25 20932 600mg 50 20934 600mg 100 20936 900mg 25 20940 900mg 50 20942 900mg 100 20944 High Capacity C18 Maxi-Clean™ Cartridges 300mg 50 20945 Large Pore C18 Maxi-Clean™ Cartridges 300mg 100 22012 600mg 100 22017 900mg 100 220215 ™ PRICE PRICE C18 96-Well Plates • Standard 96-well format • Square 2mL wells 4888 BED WEIGHT 25mg 50mg 100mg C18 96-WELL PLATES WELL VOLUME QTY. PART NO. 2.0mL 1 214996 2.0mL 1 204996 2.0mL 1 224996 PRICE Contact your Alltech office or distributor for current or local prices. SPE Reversed-Phase SPE C8 Phenyl • Choose for compounds retained too strongly on C18 C8 Specifications Functional Group: Base: Endcapped: Percent Carbon Loading: Retention Mechanism: • Choose for highly aromatic compounds Phenyl Specifications Functional Group: Base: Endcapped: Percent Carbon Loading: Retention Mechanism: Octyl (C8) 50µm irregular silica, 60Å Yes 4.5%; 8.5% high capacity Reversed-phase C8 Extract-Clean™ and Ultra-Clean™ Columns Phenyl (PH) 50µm irregular silica, 60Å Yes 3.8% Reversed-phase Phenyl Extract-Clean™ Columns 4886a C8 COLUMNS BED WEIGHT COLUMN SIZE QTY. PART NO. C8 Extract-Clean™ Columns 100mg 1.5mL 100 206000 200mg 4.0mL 50 206150 500mg 4.0mL 50 206250 500mg 8.0mL 30 206350 High Capacity C8 Extract-Clean™ Columns 100mg 1.5mL 100 206500 200mg 4.0mL 50 206550 500mg 4.0mL 50 206600 500mg 8.0mL 30 206650 C8 Ultra-Clean™ Columns 200mg 4.0mL 50 506151 500mg 4.0mL 50 506251 500mg 8.0mL 30 506351 4886a PRICE PART NO. 232062 232000 232150 232300 PRICE Phenyl Maxi-Clean™ Cartridges 4882 PHENYL MAXI-CLEAN QTY. 25 50 100 ™ BED WEIGHT 300mg 300mg 300mg C2 CARTRIDGES PART NO. 22002 22003 22004 PRICE Cyclohexyl • Choose for compounds retained too strongly on C8 C2 Specifications Functional Group: Base: Endcapped: Percent Carbon Loading: Retention Mechanism: • Choose for cyclic or aromatic compounds Cyclohexyl Specifications Functional Group: Base: Endcapped: Percent Carbon Loading: Retention Mechanism: Ethyl (C2) 50µm irregular silica, 60Å Yes 5.5% Reversed-phase C2 Extract-Clean™ Columns COLUMN SIZE 1.5mL 4.0mL 4.0mL 75mL C2 COLUMNS QTY. 100 50 50 16 © Copyright 2004 Alltech Associates, Inc. Cyclohexyl (CH) 50µm irregular silica, 60Å Yes 3.5% Reversed-phase Cyclohexyl Extract-Clean™ Columns 4886a BED WEIGHT 100mg 200mg 500mg 10,000mg PHENYL COLUMNS COLUMN SIZE QTY. 1.5mL 100 1.5mL 100 4.0mL 50 4.0mL 50 BED WEIGHT 50mg 100mg 200mg 500mg PART NO. 207300 207450 207550 207616 4886a PRICE BED WEIGHT 200mg 500mg 1000mg CYCLOHEXYL COLUMNS COLUMN SIZE QTY. PART NO. 4.0mL 50 207150 4.0mL 50 207250 8.0mL 30 207331 PRICE Contact your Alltech office or distributor for current or local prices. 13 SPE Normal-Phase SPE Cyanopropyl Aminopropyl • Choose for highly to moderately polar compounds • Choose for polar compounds, or as a weak anion exchanger Aminopropyl Specifications Functional Group: Base: Endcapped: Percent Carbon Loading: Retention Mechanism: Aminopropyl (NH2) 50µm irregular silica, 60Å No 5% Weak anion exchange w/aqueous matrix, normal-phase with non-polar organic matrix Cyanopropyl Specifications Functional Group: Base: Endcapped: Percent Carbon Loading: Retention Mechanism: Cyanopropyl (CN) 50µm irregular silica, 60Å Yes 6% Weak/moderate non-polar with aqueous matrix, or polar with nonpolar organic matrix Cyanopropyl Extract-Clean™ Columns Aminopropyl Extract-Clean™ and Ultra-Clean™ Columns 4886a 4886a AMINOPROPYL COLUMNS PART NO. QTY. COLUMN SIZE BED WEIGHT Aminopropyl Extract-Clean™ Columns 100 1.5mL 100mg 211000 50 4.0mL 200mg 211025 50 4.0mL 500mg 211150 30 8.0mL 500mg 211256 30 8.0mL 1000mg 211153 Aminopropyl Ultra-Clean™ Columns 50 4.0mL 200mg 511025 50 4.0mL 500mg 511150 PRICE CYANOPROPYL COLUMNS PART NO. QTY. COLUMN SIZE 100 1.5mL 209300 50 4.0mL 209450 50 4.0mL 209550 30 8.0mL 209650 PRICE Diol • Choose for moderately polar compounds Diol Specifications Functional Group: Base: Endcapped: Percent Carbon Loading: Retention Mechanism: Aminopropyl Extended Volume Columns Diol (2OH) 50µm irregular silica, 60Å No 4% Normal-phase Diol Extract-Clean™ Columns 6305 BED WEIGHT 500mg BED WEIGHT 100mg 200mg 500mg 500mg AMINOPROPYL EXTENDED VOLUME COLUMNS PART NO. QTY. COLUMN SIZE 30 10.0mL 407250 PRICE 4886a Aminopropyl Maxi-Clean Cartridges ™ COLUMN SIZE 1.5mL 4.0mL 4.0mL BED WEIGHT 100mg 200mg 500mg DIOL COLUMNS QTY. 100 50 50 4882 AMINOPROPYL MAXI-CLEAN™ CARTRIDGES PART NO. QTY. BED WEIGHT 50 300mg 210044 100 300mg 210046 100 900mg 210047 PRICE PRICE PART NO. 208000 208150 208250 Diol Maxi-Clean™ Cartridges 4882 DIOL MAXI-CLEAN CARTRIDGES PART NO. QTY. 50 210084 100 210086 ™ BED WEIGHT 300mg 300mg © Copyright 2004 Alltech Associates, Inc. 14 PRICE Contact your Alltech office or distributor for current or local prices. SPE Normal-Phase SPE Silica • Choose for highly polar compounds Silica Specifications Functional Group: Base: Percent Carbon Loading: Retention Mechanism: Silica Maxi-Clean™ Cartridges None 50µm irregular silica, 60Å 0% Normal-phase 4882 Silica Extract-Clean and Ultra-Clean Columns ™ ™ 4886 SILICA COLUMNS QTY. BED WEIGHT COLUMN SIZE EPA METHOD Silica Extract-Clean™ Columns 100 — 1.5mL 50mg 100 — 1.5mL 100mg 50 — 4.0mL 200mg 50 — 4.0mL 500mg 30 3630C 8.0mL 500mg 30 3630C 8.0mL 1000mg 30 — 8.0mL 2000mg 30 3630C 15mL 2000mg 20 — 25mL 5000mg 20 — 25mL 10,000mg 16 — 75mL 10,000mg 16 — 75mL 20,000mg 16 — 75mL 30,000mg Silica Ultra-Clean™ Columns 50 — 4.0mL 200mg 30 3630C 8.0mL 500mg 20 — 25.0mL 5000mg PART NO. PRICE 209062 209000 209150 209250 209200 209100 209202 209362 22935 239300 239310 239322 239330 BED WEIGHT 300mg 300mg 300mg 600mg 600mg 600mg 900mg 900mg 900mg SILICA MAXI-CLEAN™ CARTRIDGES PART NO. QTY. 25 20972 50 20974 100 20976 25 20980 50 20982 100 20984 25 20988 50 20990 100 20992 PRICE Silica 96-Well Plates 4886 BED WEIGHT 25mg 50mg 100mg 509150 509225 509010 SILICA 96-WELL PLATES PART NO. QTY. WELL VOLUME 1 2.0mL 219096 1 2.0mL 209096 1 2.0mL 229096 PRICE Silica Extended Volume Columns Could your sample results be improved by a more inert SPE column? 6305 BED WEIGHT 100mg 200mg 500mg SILICA EXTENDED VOLUME COLUMNS PART NO. QTY. COLUMN SIZE EPA METHOD 30 — 10.0mL 409001 30 — 10.0mL 409150 30 3630C 10.0mL 409250 © Copyright 2004 Alltech Associates, Inc. PRICE Choose Ultra-Clean™ columns for critical applications that can benefit from an inert glass-like column. Contact your Alltech office or distributor for current or local prices. 15 SPE Normal-Phase SPE Florisil® and Florisil®-PR Alumina • Choose for pesticides and metal organics. Florisil®-PR is tested specifically for chlorinated pesticides Florisil® Specifications Sorbent: Base: Particle Size: Retention Mechanism: Florisil® Magnesium Silicate, 60Å porosity 75-150µm Normal-phase Florisil® Extract-Clean™ and Ultra-Clean™ Columns • Alumina-Acidic—Wash with acidic solution to increase capacity for neutral or anionic compounds • Alumina-Basic—Wash with basic solution to increase capacity for cationic compounds • Alumina-Neutral—Neutral surface interacts with highly aromatic compounds and neutral hydroxyls Alumina Specifications Sorbent: Base: pH Particle Size: Retention Mechanism: 4886a FLORISIL AND FLORISIL -PR COLUMNS BED WEIGHT COLUMN SIZE EPA METHOD QTY. PART NO. Florisil® Extract-Clean™ Columns 100mg 1.5mL — 100 204500 500mg 4.0mL 3620B 50 204650 1000mg 8.0mL 3620B 30 207930 2000mg 15mL 3620B 30 207962 5000mg 25mL — 20 227950 10,000mg 75mL — 16 237910 Florisil® Ultra-Clean™ Columns 500mg 4.0mL 3620B 50 507851 1000mg 8.0mL 3620B 30 507930 Florisil®-PR Extract-Clean™ Columns 500mg 4.0mL — 50 250015 1000mg 8.0mL — 30 250020 10,000mg 75mL — 16 250910 Florisil®-PR Ultra-Clean™ Columns 500mg 4.0mL — 50 504651 1000mg 8.0mL — 30 507900 ® Alumina Aluminum Oxide, 100Å porosity 4.5 (Acidic), 10.0 (Basic), 7.5 (Neutral) 130µm Lewis acid/base, polar, and ion exchange ® PRICE Alumina Extract-Clean™ Columns 4886a ALUMINA TYPE Acidic Acidic Basic Basic Neutral Neutral Neutral Florisil® Extended Volume Columns ALUMINA EXTRACT-CLEAN™ COLUMNS BED WEIGHT COLUMN SIZE QTY. PART NO. 100mg 1.5mL 100 228200 500mg 4.0mL 50 228350 100mg 1.5mL 100 228000 500mg 4.0mL 50 228150 100mg 1.5mL 100 228400 500mg 4.0mL 50 228550 2000mg 15mL 30 22856 Alumina Extended Volume Columns 6305 BED WEIGHT 100mg 500mg PRICE 6305 FLORISIL® EXTENDED VOLUME COLUMNS COLUMN SIZE EPA METHOD QTY. PART NO. 10.0mL — 30 407600 10.0mL 3620B 30 407850 PRICE Florisil® Maxi-Clean™ Cartridges ALUMINA EXTENDED VOLUME COLUMNS ALUMINA TYPE BED WEIGHT COLUMN SIZE QTY. PART NO. Acidic 500mg 10.0mL 30 410450 Basic 500mg 10.0mL 30 410250 Neutral 500mg 10.0mL 30 410850 Neutral 11% 500mg 10.0mL 30 410730 deactivated* PRICE * Larger package sizes are available. Alumina Maxi-Clean™ Cartridges 4882 FLORISIL® MAXI-CLEAN™ CARTRIDGES BED WEIGHT QTY. PART NO. Florisil® Maxi-Clean™ Cartridges 300mg 50 210054 300mg 100 210056 900mg 50 210057 900mg 100 210061 Florisil®-PR Maxi-Clean™ Cartridges 300mg 50 210074 300mg 100 210076 900mg 50 210079 900mg 100 210075 © Copyright 2004 Alltech Associates, Inc. 16 PRICE 4882 ALUMINA TYPE Acidic Acidic Basic Basic Neutral Neutral ALUMINA MAXI-CLEAN™ CARTRIDGES BED WEIGHT QTY. PART NO. 300mg 25 210094 1800mg 25 210097 300mg 25 210093 1800mg 25 210096 300mg 25 210095 1800mg 25 210098 PRICE Contact your Alltech office or distributor for current or local prices. SPE Specialty SPE DVB Carbograph DVB Extract-Clean™ Columns Carbograph Extract-Clean™ and Ultra-Clean™ Columns • Greater sample capacity than C18 • 100% divinylbenzene reduces swelling • 40µm average particle size • Graphitized carbon retains polar organics in aqueous matrices • Acid, base-neutral extraction of pesticides and herbicides • 100m2/g surface area 4884 Extract-Clean™ DVB is useful in a broad range of applications, such as extracting phenols and acidic pesticides from environmental samples. It is also useful for polar drug metabolites and nucleic acid preparations. The presence of free vinyl groups also makes the resin useful as a solid-phase synthesis support. 4891 DVB EXTRACT-CLEAN COLUMNS COLUMN SIZE QTY. PART NO. 1.5mL 100 220025 1.5mL 100 220050 1.5mL 100 220100 4.0mL 50 220500 ™ BED WEIGHT 25mg 50mg 100mg 500mg PRICE Drying Tubes Maxi-Clean™ and Extract-Clean™ Drying Tubes CARBOGRAPH COLUMNS BED WEIGHT COLUMN SIZE QTY. PART NO. Carbograph Extract-Clean™ Columns 150mg 4.0mL 50 210142 300mg 8.0mL 30 210101 500mg 8.0mL 30 210150 1000mg 15mL 20 210121 Carbograph Ultra-Clean™ Columns 150mg 4.0mL 50 510142 300mg 8.0mL 30 510101 PRICE These SPE tubes are packed with anhydrous sodium sulfate to remove residual water from SPE extracts. They are suitable for pesticide analysis. Phase Separator Columns 4884 DEVICE Maxi-Clean™ Cartridge, 2g Extract-Clean™ Column, 3g DRYING TUBES QTY. 100 100 4895 PART NO. 219001 219002 PRICE Use Phase Separator Columns for fast and simple separation of organic and aqueous mixed samples. The column contains a 20µm polyethylene frit and a hydrophobic silicone membrane that allows the hydrophobic phase to pass through, while the aqueous phase is retained in the upper chamber. Sephadex™ SPE Columns Sephadex™ columns are commonly used for desalting procedures and for size-exclusion. - G-25 for 1000-5000 molecular weight range. - G-50 for 1500-30,000 molecular weight range. DESCRIPTION G-25 G-50 SEPHADEX™ SPE COLUMNS BED VOLUME COLUMN SIZE QTY. PART NO. 1.0mL 4.0mL 50 201200 1.0mL 4.0mL 50 201300 © Copyright 2004 Alltech Associates, Inc. PRICE 4892 COLUMN SIZE 4.0mL 8.0mL 25mL PHASE SEPARATOR COLUMNS QTY. PART NO. 100 205289 100 205389 100 205589 PRICE Contact your Alltech office or distributor for current or local prices. 17 SPE Ion-Exchange SPE SCX SAX SAX Specifications Functional Group: Base: Capacity: Retention Mechanism: Tetramethyl Ammonium Styrene-divinylbenzene 0.15meq/100mg Strong anion exchanger in acetate form; retains (-) charged compounds. SAX Extract-Clean™ Columns SCX Specifications Functional Group: Base: Capacity: Retention Mechanism: Benzenesulfonic Acid Styrene-divinylbenzene 0.24meq/100mg Strong cation exchanger in hydrogen form; retains (+) charged compounds. SCX Extract-Clean™ Columns 4889 4889 SAX COLUMNS QTY. COLUMN SIZE BED WEIGHT SAX Extract-Clean™ Columns 100 1.5mL 100mg 50 4.0mL 200mg 50 4.0mL 500mg 30 8.0mL 1000mg SAX Ultra-Clean™ Columns 50 4.0mL 200mg 50 4.0mL 500mg PART NO. PRICE 209600 209625 209750 209850 509826 509951 SCX COLUMNS QTY. COLUMN SIZE BED WEIGHT SCX Extract-Clean™ Columns 100 1.5mL 100mg 50 4.0mL 200mg 50 4.0mL 500mg 30 8.0mL 1000mg SCX Ultra-Clean™ Columns 50 4.0mL 200mg 50 4.0mL 500mg 209800 209825 209950 209930 509626 509751 6305a 6305a SAX EXTENDED VOLUME COLUMNS PART NO. QTY. COLUMN SIZE 30 10.0mL 409750 PRICE SAX Maxi-Clean™ Cartridges SCX EXTENDED VOLUME COLUMNS PART NO. QTY. COLUMN SIZE 30 10.0mL 409950 BED WEIGHT 500mg BED WEIGHT 600mg 600mg 600mg PRICE SAX 96-Well Plates 4897 BED WEIGHT 600mg 600mg 600mg SCX MAXI-CLEAN™ CARTRIDGES PART NO. QTY. 25 21901 50 21902 100 21903 SAX 96-WELL PLATES PART NO. QTY. WELL VOLUME 1 2.0mL 219696 1 2.0mL 209696 1 2.0mL 229696 © Copyright 2004 Alltech Associates, Inc. 18 PRICE SCX 96-Well Plates 4888 BED WEIGHT 25mg 50mg 100mg PRICE SCX Maxi-Clean™ Cartridges 4897 SAX MAXI-CLEAN™ CARTRIDGES PART NO. QTY. 25 21906 50 21907 100 21908 PRICE SCX Extended Volume Columns SAX Extended Volume Columns BED WEIGHT 500mg PART NO. PRICE 4888 BED WEIGHT 25mg 50mg 100mg SCX 96-WELL PLATES PART NO. QTY. WELL VOLUME 1 2.0mL 219896 1 2.0mL 209896 1 2.0mL 229896 PRICE Contact your Alltech office or distributor for current or local prices. SPE IC SPE Maxi-Clean™ IC • Eliminate matrix interferences before ion analysis • Seven chemistries solve a variety of specific problems The most difficult part of many IC applications is eliminating interfering components from the sample matrix. These interferences may co-elute or mask peaks of interest, overload the column, or shorten the column life by binding irreversibly to the column packing. Alltech’s unique ion-exchange SPE cartridges eliminate many of these interferences1, 2. Each metal-free polypropylene cartridge contains 0.5mL or 1.5mL of purified polystyrene resin, contained by 20µm polyethylene frits. The resin is functionalized to retain specific types of components from the sample. 4893 R. Saari-Nordhaus, J.M. Anderson, Jr., and I.K. Henderson, Am. Lab. August (1990) 19. 2 I.K. Henderson, R. Saari-Nordhaus, and J.M. Anderson, Jr., J. Chromatogr. 546 (1991) 61. 1 IC SPE Specifications RESIN Polystyrene IC-OH Strong anion exchanger in hydroxide form IC-H Strong cation exchanger in H+ form Cations 0.8 / 2.0 IC-Ag Strong cation exchanger in Ag+ form Strong cation exchanger in Ba+2 form Strong cation exchanger in Na+ form Chloride Iodide Bromide Sulfate 0.8 / 2.0 Cations 0.8 / 2.0 Styrene-divinylbenzene containing chelating group (iminodiacetate in Na+ form) Polystyrene/strong anion exchanger in hydroxide form Polystyrene/strong cation exchanger in hydrogen form Polyvalent metal ions 0.2 / 0.5 Hydrophobic components and anions Hydrophobic components and cations Variable-0.6 Simultaneously removes hydrophobic components and anions and increases the pH of acidic samples. Variable-0.8 Simultaneously removes hydrophobic components and cations and reduces the pH of basic samples. IC-Ba IC-Na IC-Chelate Mixed-Mode RP-OH Mixed-Mode RP-H RETAINS Hydrophobic components Anions CAPACITY (MEQ) 0.5ML/1.5ML Variable CARTRIDGE IC-RP Looking for Vacuum Manifolds? See pages 24-25 for vacuum manifolds to process samples. 0.6 / 1.5 0.8 / 2.0 APPLICATIONS Removes surfactants, organic acids, and other organic substances. Inorganic ions pass through. Exchanges anions for hydroxide. May be used to remove or concentrate anions from sample and to increase pH of acidic samples. Removes cations that form insoluble hydroxide salts. Exchanges cations for H+. May be used to remove or concentrate cations from sample and to reduce pH of basic samples. Removes excess halides through formation of Ag-halide salts. Removes excess sulfate through formation of BaSO4. Exchanges cations for Na+. May be used to remove or retain cations from sample without changing the pH of the sample. Exchanges transition metals and divalent cations for Na+. May be used to remove or retain divalent cations and transition metals from sample. For our full selection of IC columns, consumables, and instruments see pages 304-327. 4870 © Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 19 SPE IC SPE IC SPE Extract-Clean™ Columns IC SPE Maxi-Clean™ Cartridges • Process multiple samples with vacuum manifold • Two bed volumes available • For labs with higher sample throughput requirements • Use with any luer tip syringe • Two bed volumes available 4893 IC EXTRACT-CLEAN™ COLUMNS RESIN COLUMN SIZE QTY. PART NO. 0.5mL Bed Volume IC-RP 4.0mL 50 40260 IC-OH 4.0mL 50 40262 IC-H 4.0mL 50 40264 IC-Ag 4.0mL 50 105050 IC-Ba 4.0mL 50 40268 IC-Na 4.0mL 50 40270 IC-Chelate 4.0mL 50 40250 1.5mL Bed Volume IC-RP 4.0mL 30 140252 IC-OH 4.0mL 30 140254 IC-H 4.0mL 30 140256 IC-Ag 4.0mL 30 140258 IC-Ba 4.0mL 30 140261 IC-Na 4.0mL 30 140263 IC-Chelate 4.0mL 30 140265 PRICE Organic and hydrophobic contaminants may not interfere with your chromatography, but they can reduce the life of your expensive analytical columns. The photo below shows the contaminants removed from betadine, while the chromatogram shows that using IC-RP to remove these contaminants does not interfere with your separation. IC MAXI-CLEAN™ CARTRIDGES RESIN QTY. PART NO. 0.5mL Bed Volume IC-RP 50 30260 IC-OH 50 30262 IC-H 50 30264 IC-Ag 50 30266 IC-Ba 50 30268 IC-Na 50 30270 IC-Chelate 50 30250 Mixed-Mode RP-OH 25 30259 Mixed-Mode RP-H 25 30267 1.5mL Bed Volume IC-RP 25 30252 IC-OH 25 30254 IC-H 25 30256 IC-Ag 25 30258 IC-Ba 25 30261 IC-Na 25 30263 IC-Chelate 25 30265 PRICE IC-RP Protects Analytical Columns by Removing Hydrophobic Contaminants Iodide in Betadine Sample After Treatment with IC-RP 5 12 0 3 1. 2. 3. 4. 5. Fluoride Chloride Phosphate Sulfate Iodide 13003 4 4 8 12 Min. Column: IC-RP bed before extraction IC-RP bed after extraction Novosep™ Anion, 7µm, 100 x 4.6mm HPLC Column (Part No. 51406) Mobile Phase: 5mM Sodium Carbonate Flow Rate: 1.0mL/min Detector: Suppressed Conductivity 6381 © Copyright 2004 Alltech Associates, Inc. 20 Contact your Alltech office or distributor for current or local prices. SPE IC SPE IC SPE Applications IC-Ag Removes Chloride IC-Ba Removes Sulfate Nitrite in Brine Anions in Battery Acid 10059 10060 Unextracted 10064 Extracted 1. Fluoride 2. Nitrate 3. Sulfate 10065 Unextracted Extracted 1. 2. 3. 4. 5. � � 5 Fluoride Chloride Nitrite Nitrate Sulfate 2 � 0 4 8 12 16 20 min. � 5 1 � � �� �� ������� 0 4 8 12 16 20 Min. 0 34 4 8 16 20 Min. Column: Column: Novosep™ Anion, 7µm, 150 x 4.6mm IC Column (Part No. 51407) Mobile Phase: 1.7mN NaHCO3/1.8mM Na2CO3 Flow Rate: 1.5mL/min Detector: Conductivity (Suppressed) Novosep™ A1, 7µm, 150 x 4.6mm IC Column (Part No. 51407) Mobile Phase: 1.7mM NaHCO3/1.8mM Na2CO3 Flow Rate: 1.2mL/min Detector: Conductivity (Suppressed) IC-H Neutralizes Hydroxide IC-OH Neutralizes Acid Trace Anions in Caustic Nitric Acid 10069 10070 Unextracted 12 Extracted 10062 10063 Unextracted 1. Fluoride 2. Chloride 3. Nitrate Extracted 1. Sodium 2. Magnesium 3. Calcium � � � � � � � � � ������� Column: � � � � Novosep™ A1, 7µm, 150 x 4.6mm IC Column (Part No. 51407) Mobile Phase: 1.7mM NaHCO3 / 1.8mM Na2CO3 Flow Rate: 1.2mL/min Detector: Conductivity (Suppressed) © Copyright 2004 Alltech Associates, Inc. �� � ������� � � � � � �� �� ������� � � � � � �� �� ������� Column: Universal Cation, 7µm, 100 x 4.6mm IC Column (Part No. 27106) Mobile Phase: 3mM Methanesulfonic Acid Flow Rate: 1.0mL/min Detector: Conductivity (Non-Suppressed) Contact your Alltech office or distributor for current or local prices. 21 SPE Custom SPE and Accessories Custom Packed SPE Products While we list the most popular sorbent and column combinations in our standard product line, we realize that there are many new and exciting applications that may require something special. SPE Custom Quote Request to Alltech Date: FAX To: 814-234-3594 Or Email: [email protected] Alltech will custom pack any of our columns or cartridges to your specifications. We will consider all requests, large or small. FAX or email your custom request on your letterhead for a price quotation. Be sure to include a contact name and phone number. Please specify: 1. Packing Material 2. Quantity Per Device 3. Device Size and Style Custom orders are not returnable. A minimum order may be required. Contact Name: Contact Phone Number: Alltech Part No.: C-27100 - Custom Order Packing Material: Amount of Packing Material: Device Size: Device Style: Extract-Clean™ Column (choose one) Maxi-Clean™ Cartridge Ultra-Clean™ Treated Column Extended Volume Column Total Pieces Required: Special Instructions: Bulk SPE Packings Filter Columns These bulk packings are the same high-quality material we manufacture for our Extract-Clean™ and Maxi-Clean™ SPE products. Filter columns are Extract-Clean™ reservoirs with two frits at the outlet end. They remove particulate matter down to 20µm from samples. Syringe adapters will connect filter columns to the tops of Extract-Clean™ columns. 4900 BULK SPE PACKINGS DESCRIPTION QTY. Reversed-Phase Adsorbents Prevail C18 (11%) 100g C18 (6%) 100g C18 (17%) 100g Large Pore C18 (14%) 100g C8 100g C2 100g Phenyl 100g Normal-Phase Adsorbents Silica 100g Aminopropyl 100g Diol 100g Florisil® 227g Florisil® PR 227g Alumina-N 100g Ion Exchange SCX (Cation Exchange) 100g SAX (Anion Exchange) 100g 25g Carbograph 1 SPE 120/400 Mesh © Copyright 2004 Alltech Associates, Inc. 22 PART NO. 211505 211502 211503 211542 211504 211506 211522 PRICE EXTRACT-CLEAN™ FILTER COLUMNS DESCRIPTION QTY. PART NO. 1.5mL Filter Columns 100 211101 4.0mL Filter Columns 50 211104 8.0mL Filter Columns 50 211108 75.0mL Filter Columns 50 210775 PRICE 211512 211516 211518 5618 5615 211541 211520 211510 1769 See pages 2-5 for help choosing a sorbent and device. Contact your Alltech office or distributor for current or local prices. SPE SPE Accessories Reservoirs and Frits Adapters & Caps • Inlet caps fit SPE devices as indicated. • Outlet caps fit any column or cartridge with a male luer tip. Select empty reservoirs and loose frits to pack your own custom SPE columns. BULK RESERVOIRS AND FRITS DESCRIPTION QTY. PART NO. Extract-Clean™ Empty Reservoirs, Polypropylene 1.5mL 100 210001 1.5mL 500 210011 4.0mL 100 210104 8.0mL 100 210208 15.0mL 100 210315 25.0mL 100 210425 75.0mL 50 210575 Extended Volume Empty Reservoirs, Polypropylene Up to 10.0mL Sample Volume 100 430050 Up to 10.0mL Sample Volume 1000 420011 Ultra-Clean™ Empty Reservoirs, Treated Polypropylene 4.0mL 100 70216 8.0mL 100 70218 8.0mL 1000 70069 20µm Polyethylene Frits for Extract-Clean™ For 1.5mL Reservoir 100 211401 For 4.0mL or EV Reservoir 100 211404 For 8.0mL Reservoir 100 211408 For 15.0mL Reservoir 100 211412 For 25.0mL Reservoir 100 211416 For 75.0mL Reservoir 100 211775 10µm PTFE Frits for Ultra-Clean™ For 4.0mL Reservoir 100 211494 For 8.0mL Reservoir 100 211498 6288 PRICE 5952 CAPS DESCRIPTION Inlet Caps for Maxi-Clean™ Cartridges Inlet Caps for 1.5mL Inlet Caps for 4.0mL Inlet Caps for 8.0mL Inlet Caps for 15.0mL or EV Inlet Caps for 25.0mL Inlet Caps for 75.0mL Outlet Caps for Male Luers • Square, 2mL wells • All-polypropylene construction • Use to pack your own custom SPE plates SYRINGE ADAPTERS DESCRIPTION QTY. Syringe Adapters for 1.5mL, 4.0mL, 15 8.0mL, 15mL, and EV Columns PART NO. 210705 Syringe Adapters for 25mL Columns Syringe Adapters for 75mL Columns 210707 210709 4888 PRICE 5 5 PRICE Looking for One-Step Mobile Phase Clean-Up? Standard 96-well plate footprint (8x12 wells) PART NO. 210780 PART NO. 211015 220000 220301 220600 221200 221006 227503 220710 Syringe Adapters fit the tops of SPE columns allowing attachment of any male luer device. This lets you process an Extract-Clean™ column with a syringe or add an empty reservoir to increase sample volume capacity. Empty 96-Well Plates 96-WELL PLATES BED WEIGHT QTY. Empty plate with bottom frit 1 installed, top frit supplied QTY. 50 50 50 50 50 50 50 50 Try SuperClean™ SPE columns on page 39. PRICE 5953 © Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 23 SPE SPE Accessories Alltech Vacuum Manifolds I • 12-, 16-, and 24-port manifolds • Glass chamber for visual monitoring • Accepts standard male luer devices Vacuum manifolds process multiple samples simultaneously, saving time and effort. Manifold systems come complete with the components listed below. Stainless steel or PTFE needles are available separately. C F H E A J B E I D G 6278 12-Port Manifold Vacuum Manifold Components A. B. C. D. E. F. G. H. I. J. • For up to 12 samples • Also includes one waste container 4869 12-PORT MANIFOLD & ACCESSORIES DESCRIPTION QTY. PART NO. ea 12-Port Vacuum Manifold** 210351 Replacement Parts Lid, Gaskets, and 12 Stopcocks ea 212001 Glass Chamber ea 213212 Vacuum Gauge, Valve, & Glass Chamber ea 212304 Collection Rack, 12-Port Size* ea 212518 Gaskets, 12-Port Size 2 212112 One-Way Stopcocks 12 213112 Waste Container 2 210033 PRICE Glass Chamber Vacuum Valve and Gauge Polypropylene Lid Stopcock Valves Collection Rack Plates Support Posts for Collection Racks Retaining Clips for Collection Racks Lid Legs Manifold Inlet Caps Polypropylene Needles 24-Port Manifold • Process up to 24 samples *12-Port Collection Rack includes 3 support posts, bottom plate, 13- and 16mm plates, autosampler vial plate, volumetric plate, and 12 retaining clips. ** Includes waste container. 4871 16-Port Manifold 24-PORT MANIFOLD & ACCESSORIES DESCRIPTION QTY. PART NO. ea 24-Port Vacuum Manifold 210224 Replacement Parts Lid, Gaskets, and 24 Stopcocks ea 211224 Glass Chamber ea 210124 Vacuum Gauge, Valve, & Glass Chamber ea 210324 Collection Rack, 24-Port Size† ea 210424 Gaskets, 16- and 24-Port Size 2 210724 One-Way Stopcocks 24 211524 • Process up to 16 samples 4870 16-PORT MANIFOLD & ACCESSORIES DESCRIPTION QTY. PART NO. ea 16-Port Vacuum Manifold 210016 Replacement Parts Lid, Gaskets, and 16 Stopcocks ea 210216 Glass Chamber ea 210116 Vacuum Gauge, Valve, & Glass Chamber ea 210316 Collection Rack, 16-Port Size† ea 210416 Gaskets, 16- and 24-Port Size 2 210724 One-Way Stopcocks 16 211521 © Copyright 2004 Alltech Associates, Inc. 24 PRICE PRICE 16- and 24-Port Collection Racks include 3 support posts, bottom plate, dimple plate, 13- and 16mm plates, and 12 retaining clips. † Contact your Alltech office or distributor for current or local prices. SPE SPE Accessories Manifold Accessories Replacement Parts for All Size Manifolds Drying Attachments Manifolds come complete with all necessary gauges and accessories. Individual replacement parts may be sold separately. Use these special drying lids to direct a gas flow into collection tubes to dry eluants. Barb is 1/4". 5951 REPLACEMENT PARTS FOR ALL SIZE MANIFOLDS DESCRIPTION QTY. PART NO. Vacuum Gauge and Valve ea 212203 Retaining Clips For Collections Racks 12 212912 Female Luer Inlet, Polypropylene 2 212002 Female Luer Inlet, Polypropylene 24/pk 212302 Male Luer Outlet, Polypropylene 2 212120 Male Luer Outlet, Polypropylene 24/pk 212320 Caps for Lid Inlets 50 211234 Lid Legs, Black Delrin 4 410510 Collection Rack Posts, Polypropylene 3 410410 PRICE 4878 DRYING ATTACHMENTS DESCRIPTION QTY. 12-Port Drying Attachment 1 16-Port Drying Attachment 1 24-Port Drying Attachment 1 PART NO. 212100 212117 212124 PRICE Manifold Needles Tygon® Tubing Tygon® vacuum tubing is heavy walled and flexible. It withstands 29.9" Hg at 75°F (24°C). It is nontoxic, nonoxidizing, and transparent. PTFE needles are disposable, fit many different manifold types, and eliminate cross contamination by extending into the collection tube. They also provide a complete PTFE fluid path for samples to virtually eliminate extractables. Available plain or with a valve. Stainless Steel and Polypropylene needles are also available. Stainless Steel PTFE Polypropylene Valved PTFE 4874 3849 TYGON® VACUUM TUBING DESCRIPTION QTY. PART NO. 1/8" i.d., 3/8" o.d. 50' 6465 3/16" i.d., 9/16" o.d. 10' 6467 1/4" i.d., 3/4" o.d. 10' 6469 3/8" i.d., 7/8" o.d.* 10' 6472 *Use with Vacuum Gauge and Valve displayed above. © Copyright 2004 Alltech Associates, Inc. PRICE DESCRIPTION PTFE Needles PTFE Needles Valved PTFE Needles Valved PTFE Needles Stainless Steel Needles Stainless Steel Needles Stainless Steel Needles Polypropylene Needles Polypropylene Needles Polypropylene Needles MANIFOLD NEEDLES QTY. 100 500 25 50 12 16 24 12 16 24 PART NO. 412410 412450 411525 411550 212400 210816 210824 212412 210916 210924 PRICE Contact your Alltech office or distributor for current or local prices. 25 SPE SPE Accessories Benchtop Vacuum Station Vacuum/Pressure Station • Pulls vacuum to 28.5" Hg • Portable and low maintenance • Continuous LCD display of vacuum level • Pulls vacuum to 20" Hg, pressure to 18 psig • Extended life diaphragm • No vacuum oil required • Eliminates need for water aspirators 4178 5691 Alltech’s Benchtop Vacuum Station is a versatile, powerful, user-friendly answer to most vacuum applications. Three modes of operation offer flexibility for different applications. “Continuous Vacuum Mode” pulls a maximum vacuum with a 1-120 minute timer. “Continuous Vent Mode” clears the pump heads of vapors. “Set Point Mode” allows you to select the vacuum level, and it maintains that vacuum level with an absolute vacuum sensor, making runs reproducible regardless of altitude or barometric conditions. It is the perfect pump for filtration and solid phase extraction tasks. This Vacuum/Pressure station is an economical choice for the busy lab. By simply switching ports, it can be changed from a vacuum source to a pressure station. Benchtop Vacuum Station Specifications 725mm Hg (28.5") Maximum Pull: 6L/min Flow Rate: 1-120 minutes Timer: 8.7" W x 9.5" D x 4.5" H Dimensions: (22cm W x 24.2cm D x 11.5cm H) Vacuum/Pressure Station Specifications 20" Hg Vacuum Pull: 18psig Pressure: 0.5cu. ft/min Free Air Capacity: 8.75" H x 8" W x 7.5" L Dimensions: (22.23cm H x 20.32cm W x 19.05cm L) DESCRIPTION 115V 230V BENCHTOP VACUUM STATION PART NO. 6417 6419 PRICE The unit combines a single-head pump, vacuum/ pressure gauge, and stainless steel regulating valves. DESCRIPTION 115V 230V VACUUM/PRESSURE STATION PART NO. 1670 1595 PRICE VACUUM/PRESSURE REPLACEMENT PARTS/ACCESSORIES DESCRIPTION PART NO. PRICE Replacement Parts Vacuum/Pressure Gauge Connector Kit: 1590 Includes Vacuum Regulation Valve Assembly, Pressure Regulation Valve Assembly, and a Manifold Chamber Service Kit: Includes Plastic Top with Retainers, Valves, Nitrile Diaphragm and Eccentric Assembly Accessories Vacuum/Pressure Gauge 1592 1597 4384 © Copyright 2004 Alltech Associates, Inc. 26 Contact your Alltech office or distributor for current or local prices. F I LT R AT I O N Filtration Introduction Product Selection There are several basic steps to choosing the best filtration product for your needs. This guide will help you get started. 1. Choose a Filter Membrane Refer to the Membrane Selection Guide and the Chemical Compatibility Chart on the next pages to select the appropriate membrane for your application. Consider a.) sample particulate size b.) aqueous or organic nature of the sample, and c.) the levels of solvent resistance and protein retention you require. Filter membranes remove particulates from a sample or mobile phase. 0.45µm pores are commonly used to remove sample or mobile phase particulates prior to HPLC analysis. 0.2µm pores remove bacteria from biological samples and can be used for sterilization. Filter Capacities Filter Specifications DESCRIPTION Syringe Filter Capacities 4mm Syringe Filter 13mm Syringe Filter 17mm Syringe Filter 25mm Syringe Filter 30mm Syringe Filter Centrifuge Filter Tube Capacities Ultra-Spin™ Tubes Micro-Spin® Tubes Maxi-Spin™ Tubes Pre-Cut Membrane Capacities 13mm Pre-Cut Membranes 25mm Pre-Cut Membranes 47mm Pre-Cut Membranes 90mm Pre-Cut Membranes SAMPLE VOLUME ≤1mL ≤5mL ≤50mL ≤50mL ≤100mL 20µL to 500µL 20µL to 500µL 1mL to 25mL up to 20mL up to 100mL multi-liter multi-liter 2. Choose a Membrane Diameter Larger sample volumes require larger membrane diameters. High particulate levels will reduce the amount of sample filtered before a membrane becomes plugged, so use a larger diameter membrane or a glass pre-filter to increase filtrate volume with particulate laden samples. 3. Choose a Device Consider the number of samples, the sample volume, and the filtering mechanism you prefer to use. Syringe Filters: Filtration is achieved by pushing the sample through the membrane with a syringe or other luer-connection device. Syringe filters allow you to control the rate of flow, which can be critical with delicate samples. Syringe filters also allow you to filter into nearly any tube, vial, or column that represents the next step in your analysis. Syringe filters with glass pre-filters are used in the same manner as regular syringe filters. They are especially useful for particulate laden samples. Centrifuge Filter Tubes: Filtration is achieved by pulling the sample through the membrane with centrifugal force. This allows you to simultaneously filter as many samples as your centrifuge can hold, and captures your filtrate in a clean microtube. Pre-Cut Membranes: Multiple diameters offer a wide range of sample capacities, up to multiple liters. You need a filtration apparatus, such as a filter funnel or other vacuum-driven filtration device. What is the advantage of a glass pre-filter? Pre-filters rest on top of the filter membrane and help trap large particulates to reduce clogging, minimize sample loss, and extend the membrane life. Many of Alltech’s syringe filters are available with 1.0µm borosilicate glass pre-filters. We highly recommend using syringe filters with glass pre-filters for particulate-laden samples. ��������������� ���������������� �������� ����������������� Syringe Filter with Glass Pre-filter © Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 27 F I LT R AT I O N Filtration Introduction Filter Membrane Chemical Compatibility Compatible Limited Compatibility Not Compatible No Data Available REGENERATED CELLULOSE C C C C C C C C L C C C C C C N C N C N C C C C C C C C L C N C C C C C C L C C C C N C C N N - C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C C N C C C C C C C C C N L C C C C C C C C L C C C C C C C C C C C C C C C C C C C - C C C C C C C C C C C C C C C C C C C C C C C C C C N N L N N C C C C C N L L L L L C C C C C C C C C L C L C C C C C C C C C C C C C C C C N L N L N N N L C N C N L C L C L C L L L L L L C C L L C C C C C C C © Copyright 2004 Alltech Associates, Inc. 28 REGENERATED CELLULOSE ANOPORE™ L N L C N N N L N C C ANOPORE™ POLYPROPYLENE C C C C C C N L N C C POLYPROPYLENE CELLULOSE ACETATE C C C C C C L L L C C CELLULOSE ACETATE POLYSULFONE C C C C N C C C C C C POLYSULFONE PVDF L L N L N N N N N N L CHEMICAL Ketones Acetone Cyclohexanone Isopropylacetone Methyl Ethyl Ketone Methyl Isobutyl Ketone (MIBK) Esters 2-Ethoxyethyl Acetate Amyl Acetate Benzyl Benzoate Butyl Acetate Ethyl Acetate Isopropyl Myristate Methyl Acetate Propyl Acetate Propylene Glycol Acetate Methyl Cellosolve Acetate Tricresyl Phosphate Oxides — Ethers Acetonitrile (Methyl Cyanide) Aniline Diethyl Acetamide Dimethyl Formamide Dimethyl Sulfoxide (DMSO) Dioxane Ethyl Ether Isopropyl Ether Pyridine Solvents with Nitrogen Tetrahydrofuran Triethanolamine Miscellaneous Formaldehyde Solution, 30% Hydrogen Peroxide, 30% Phenol, Aqueous, 10% Silicone Oil & Mineral Oil PVDF PTFE CHEMICAL Acids Acetic, 25% Acetic, Glacial Formic, 25% Hydrochloric, 25% Hydrochloric, Concentrated Sulfuric, 25% Sulfuric, Concentrated Nitric, 25% Nitric, Concentrated Phosphoric, 25% Trichloroacetic, 10% Bases Ammonium Hydroxide, 25% Sodium Hydroxide, 3 Normal Alcohols Amyl Alcohol Benzyl Alcohol Butyl Alcohol Ethanol, 70% Ethanol, 98% Ethylene Glycol Glycerine (Glycerol) Isopropanol Methanol, 98% n-Propanol Propylene Glycol Hydrocarbons Benzene Hexane, Xylene Kerosene, Gasoline Tetralin, Decalin Toluene Halogenated Hydrocarbons Carbon Tetrachloride Chlorobenzene (Mono) Chloroform Freon Methylene Chloride Trichloroethane Trichloroethylene Filter Membrane Chemical Compatibility Chart NYLON Filter Membrane Chemical Compatibility Chart PTFE = = = = NYLON C L N – C C C C C C C C C C N N N L N N N N N N N N C L C C C C L C C C C C C C C L C C C C C L L L C - L C C C C C C N C C C L C C C L C C - N N N N N N N N N N N L L C L N C N N N N C L L L L L C C - L L C C L C - C C C C C C C C C C C C L C C L C C C C C C C C C C C C C C C C N C L C N L N N N N N C C N N N N N N N L C N C L C C C L C L C L L C C C C C C L L L C C C C C C L C N - N C C - C - L C C N N C C C C C C C L C C C N C L C N C C C C C L L C L C C C This is only a guide. Users should verify compatibility under actual use conditions. Contact your Alltech office or distributor for current or local prices. F I LT R AT I O N Filtration Introduction Alltech Syringe Filters More Sizes, More Choices True™ Syringe Filters High quality syringe filters with threaded luer inlets, QC tested for demanding chromatography applications. New, larger 17mm and 30mm diameters offer higher sample capacities, faster flow rates, and lower backpressures. See page 30. Specialty Syringe Filters PolyPure™ II syringe filters use a pure, hydrophobic polypropylene membrane for high solvent resistance and low extractables. See page 31. Anotop® syringe filters from Whatman use the Anopore® membrane; a unique membrane for complete particle exclusion. See page 34. 6277 HPLC Syringe Filters Traditional 13mm and 25mm diameter syringe filters for common chromatography applications. See pages 32-33. Economy Syringe Filters These 25mm syringe filters are an economical alternative for non-aggressive applications. See page 35. Membrane Selection Guide Choose a filter membrane based on the size and amount of particulate in the sample, the membrane’s chemical compatibility with the sample matrix, and potential interactions (binding) between the membrane and the sample components. This table offers general guidelines on membrane characteristics and applications. Membrane Selection Guide MEMBRANE TYPE/ PAGE Anopore® Cellulose Acetate FEATURES Offers complete particle exclusion. Contains no glue, binders, plasticizers, or monomers that can contaminate the sample. Ideal for biological applications. Nylon Good chemical compatibility and very low extractables Polypropylene (PolyPure™II) Lower protein binding than PVDF. Pre-wet with methanol for aqueous samples Compatible with strong acids and aggressive solvents. Good flow rate characteristics. Ideal for chromatography applications. Universal membrane with excellent chemical resistance and low extractables. Use for aqueous and organic samples. Rated 12-, 30-, or 100kD molecular weight cut-off. Recover low molecular weight materials from biological samples and media. Polytetrafluoroethylene (PTFE) Polyvinylidene Fluoride (PVDF) Regenerated Cellulose Ultrafilters * Highest solvent resistance. ** Lowest protein binding. † PVDF in Syringe Filters is hydrophilic; PVDF in Centrifuge Filter Tubes is hydrophobic. © Copyright 2004 Alltech Associates, Inc. COMMON USES Solvent filtration IC sample prep biological filtration HYDROPHILIC Yes SOLVENT RESISTANCE High PROTEIN BINDING Low General filtration sterilization General filtration sterilization, HPLC sample prep HPLC sample prep general filtration Yes Limited Very Low Yes Good Medium No High Low No High* High See Below† High Low Yes High Very Low** Yes Limited Low Gas, air, and solvent filtration HPLC sample preparation and general filtration HPLC sample prep general filtration Sample concentration deproteinization buffer exchange molecule recovery Contact your Alltech office or distributor for current or local prices. 29 F I LT R AT I O N Syringe Filters Alltech True™ Syringe Filters Larger 17mm and 30mm diameters for: • Higher sample capacities • Faster flow rates • Lower back pressures Alltech’s new True™ Syringe Filters have true 13mm and 25mm filter diameters inside larger housings. They are HPLC Certified for high strength and low extractables. Larger package sizes are available, and a QC sheet is included with each unit. 6735 True™ Membrane Specifications MEMBRANE MAX. TEMP Cellulose Acetate 110ºC Nylon 100ºC PTFE* 100ºC PVDF 110ºC 110ºC Regenerated Cellulose True PTFE Syringe Filters ™ TYPE Hydrophilic Hydrophilic Hydrophobic Hydrophilic Hydrophilic * True™ PTFE membranes have a polypropylene support layer True™ Device Specifications MAX. PRESSURE DEVICE 4mm 75psig 17mm 115psig 30mm 90psig HOLD-UP VOLUME less than 15µL less than 25µL less than 115µL PRICE True™ Nylon Syringe Filters MEMBRANE Nylon Nylon/Glass Nylon Nylon/Glass Nylon Nylon/Glass Nylon Nylon Nylon Nylon Nylon TRUE™ NYLON SYRINGE FILTERS DEVICE PORE DIAMETER SIZE QTY. 30mm 0.20µm 100 30mm 0.20µm 100 30mm 0.45µm 100 30mm 0.45µm 100 30mm 1.20µm 100 30mm 1.20µm 100 30mm 5.00µm 100 17mm 0.20µm 100 17mm 0.45µm 100 4mm 0.20µm 100 4mm 0.45µm 100 PRICE True™ PVDF Syringe Filters True™ Cellulose Acetate Syringe Filters TRUE™ CELLULOSE ACETATE SYRINGE FILTERS DEVICE PORE MEMBRANE DIAMETER SIZE QTY. PART NO. Cellulose Acetate 30mm 0.20µm 100 62157 Cellulose Acetate 30mm 0.45µm 100 62187 Cellulose Acetate 17mm 0.20µm 100 62131 Cellulose Acetate 17mm 0.45µm 100 62171 Cellulose Acetate 4mm 0.20µm 100 2634 Cellulose Acetate 4mm 0.45µm 100 2635 MEMBRANE PTFE PTFE PTFE PTFE PTFE PTFE PTFE PTFE TRUE™ PTFE SYRINGE FILTERS DEVICE PORE DIAMETER SIZE QTY. PART NO. 30mm 0.20µm 100 62189 30mm 0.45µm 100 62191 30mm 1.00µm 100 62137 30mm 5.00µm 100 62175 17mm 0.20µm 100 62118 17mm 0.45µm 100 62136 4mm 0.20µm 100 2394 4mm 0.45µm 100 2395 MEMBRANE PVDF PVDF PVDF PVDF PVDF PVDF TRUE™ PVDF SYRINGE FILTERS DEVICE PORE DIAMETER SIZE QTY. PART NO. 30mm 0.20µm 100 62193 30mm 0.45µm 100 62195 17mm 0.20µm 100 62130 17mm 0.45µm 100 62209 4mm 0.20µm 100 2227 4mm 0.45µm 100 2228 PRICE True™ Regenerated Cellulose Syringe Filters PART NO. 62145 62129 62147 62135 62149 62139 62165 62163 62177 2091 2092 PRICE TRUE™ REGENERATED CELLULOSE SYRINGE FILTERS DEVICE PORE MEMBRANE DIAMETER SIZE QTY. PART NO. Regenerated Cellulose 30mm 0.20µm 100 62199 Regenerated Cellulose 30mm 0.45µm 100 62167 Regenerated Cellulose 17mm 0.20µm 100 62119 Regenerated Cellulose 17mm 0.45µm 100 62125 Regenerated Cellulose 4mm 0.20µm 100 656100 Regenerated Cellulose 4mm 0.45µm 100 656102 PRICE Have a Question about Your Order? Contact Alltech Customer Service: Online Ordering 24 hours a day: www.alltechWEB.com Email: [email protected] © Copyright 2004 Alltech Associates, Inc. 30 Contact your Alltech office or distributor for current or local prices. F I LT R AT I O N Syringe Filters PolyPure™ II Syringe Filters • Hydrophobic polypropylene membrane • Super low extractables • Contains no wetting agents POLYPURE™ II SYRINGE FILTERS DEVICE TYPE, PORE SIZE QTY. PART NO. 4mm Syringe Filters 0.20µm 100 6710 0.20µm 500 6712 0.45µm 100 6714 0.45µm 500 6716 13mm Syringe Filters 0.20µm 100 6718 0.20µm 500 6720 0.45µm 100 6722 0.45µm 500 6724 25mm Syringe Filters 0.20µm 100 6730 0.20µm 500 6732 0.45µm 100 6742 0.45µm 500 6744 25mm with Glass Pre-Filter 0.20µm 100 6738 0.20µm 500 6740 0.45µm 100 6750 0.45µm 500 6752 PRICE 4782 PolyPure™ II Specifications DEVICE MAX. PRESSURE 4mm 75psig 13mm 75psig 25mm 75psig MAX. TEMPERATURE HOLD-UP VOLUME 100ºC less than 7.5µL 100ºC less than 25µL 100ºC less than 100µL Need Technical Assistance? Contact Alltech Tech Support: Email: [email protected] Online: www.alltechWEB.com Why are hydrophobic polypropylene membranes better than hydrophilic membranes? Polypropylene is a naturally hydrophobic filter membrane. Some manufacturers add a wetting agent to make polypropylene hydrophilic. These chromatograms compare extractables using isopropanol at 220nm from hydrophilic polypropylene membranes and Alltech’s pure hydrophobic PolyPure II™ membranes. The large peak present from the hydrophilic polypropylene membrane is the wetting agent, which elutes in organic solvents and can interfere with your chromatography. IPA Extraction Off Hydrophilic Polypropylene 0 2 4 6 © Copyright 2004 Alltech Associates, Inc. 8 10 12 Min. IPA Extraction Off Hydrophobic PolyPure™ II 4612 � � � � � ������� 4613 Contact your Alltech office or distributor for current or local prices. 31 F I LT R AT I O N Syringe Filters Alltech HPLC Syringe Filters Traditional 13mm and 25mm outer diameter syringe filters for general purpose chromatography applications. A QC sheet is included with each unit. HPLC Membrane Specifications MEMBRANE MAX. TEMP Cellulose Acetate 60ºC PTFE* 60ºC Nylon 60ºC 60ºC Regenerated Cellulose PVDF 60ºC TYPE Hydrophilic Hydrophobic Hydrophilic Hydrophilic Hydrophilic * HPLC PTFE membranes have a polypropylene support layer HPLC Device Specifications DEVICE MAX. PRESSURE 13mm 100psig 25mm 100psig HOLD-UP VOLUME less than 10µL less than 50µL 6737 Cellulose Acetate Syringe Filters PTFE Syringe Filters • Choose for clarification, sterilization, biological filtration, and filtration of aqueous solutions MEMBRANE 13mm 25mm CELLULOSE ACETATE SYRINGE FILTERS PORE SIZE QTY. PART NO. 0.20µm 100 2642 0.20µm 1000 605025 0.45µm 100 2643 0.45µm 1000 605023 0.20µm 100 2161 0.45µm 100 2160 • Choose for gas, air, and solvent filtration • Hydrophobic with high solvent resistance PRICE Need Help Selecting the Correct Membrane? See pages 27-29 for individual membrane specifications and suggested use/applications. MEMBRANE 13mm 25mm PTFE SYRINGE FILTERS PORE SIZE QTY. 0.20µm 100 0.20µm 1000 0.45µm 100 0.45µm 1000 0.20µm 100 0.20µm 1000 0.45µm 100 0.45µm 1000 1.00µm 100 5.00µm 100 PART NO. 2164 605017 2165 605015 2089 605710 2090 605810 2429 6542 PRICE Need Technical Assistance? Contact Alltech Tech Support: Email: [email protected] Online: www.alltechWEB.com 4777 © Copyright 2004 Alltech Associates, Inc. 32 Contact your Alltech office or distributor for current or local prices. F I LT R AT I O N Syringe Filters Regenerated Cellulose Syringe Filters Nylon Syringe Filters • Choose for HPLC sample preparation and general biological filtration • High solvent resistance and low protein binding • Choose for general filtration, sterilization, and protein binding NYLON SYRINGE FILTERS PORE SIZE QTY. 0.20µm 100 0.20µm 1000 0.45µm 100 0.45µm 1000 25mm 0.20µm 100 0.20µm 1000 0.45µm 100 0.45µm 1000 25mm w/Glass Pre-Filter 0.45µm 100 MEMBRANE 13mm PART NO. 2166 605013 2167 605011 2045 2945 2047 605410 2359 PRICE What is the advantage of a glass pre-filter? Pre-filters rest on top of the filter membrane and help trap large particulates to reduce clogging, minimize sample loss, and extend the membrane life. Many of Alltech’s syringe filters are available with 1.0µm borosilicate glass pre-filters. We highly recommend using syringe filters with glass pre-filters for particulate-laden samples. REGENERATED CELLULOSE SYRINGE FILTERS MEMBRANE PORE SIZE QTY. PART NO. 13mm 0.20µm 100 656114 0.20µm 1000 605029 0.45µm 100 656116 0.45µm 1000 605027 25mm 0.20µm 100 656124 0.45µm 100 656126 0.45µm 1000 605031 PRICE PVDF Syringe Filters • Choose for clarification, sterilization, and biological filtration MEMBRANE 13mm 25mm PVDF SYRINGE FILTERS PORE SIZE QTY. 0.20µm 100 0.20µm 1000 0.45µm 100 0.45µm 1000 0.20µm 100 0.45µm 100 PART NO. 2647 605021 2648 605019 2223 2224 PRICE ��������������� Need Larger Diameter Syringe Filters? ���������������� See Alltech’s new True™ Syringe Filters on page 30. �������� 6735 ����������������� Need Syringes? See page 38 for disposable polypropylene or glass syringes. Syringe Filter with Glass Pre-filter 4675 © Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 33 F I LT R AT I O N Syringe Filters Anotop® IC Syringe Filters Anotop® Syringe Filters • Ideal for ion chromatography - ultra low extractables • Pigment-free polypropylene housings • Available with or without glass pre-filter • Unique Anopore® membrane for complete particle exclusion 4767 Anopore® Membrane Specifications Anopore® Membrane: Hydrophilic Type: Low Protein Binding: High Solvent Resistance: Solvent filtration, IC sample prep, Common Uses: biological filtration Anopore® membranes offer complete Other Information: particle exclusion through their unique capillary pore honeycomb structure. ANOTOP® SYRINGE FILTERS DEVICE TYPE, PORE SIZE QTY. PART NO. 10mm Syringe Filters 0.02µm 50 2172 0.10µm 50 2240 0.20µm 50 2170 10mm Syringe Filters with Glass Pre-Filter 0.02µm 50 2287 0.10µm 50 2285 0.20µm 50 2283 25mm Syringe Filters 0.10µm 50 2252 0.20µm 50 2130 25mm Syringe Filters with Glass Pre-Filter 0.02µm 50 2262 0.10µm 50 2270 0.20µm 50 2260 Anotop® Syringe Filter Capacities DESCRIPTION HOLDUP VOLUME 10mm Syringe Filter <20µL 25mm Syringe Filter <150µL SAMPLE VOLUME 40µL to 10mL 200µL to 100mL 0417 Anopore® Inorganic Membrane PRICE 0418 Typical Polymeric Membrane 0419 Unique Anopore® Surface Retention Have a Question about Your Order? Contact Alltech Customer Service: Online Ordering 24 hours a day: www.alltechWEB.com Email: [email protected] © Copyright 2004 Alltech Associates, Inc. 34 ANOTOP® IC SYRINGE FILTERS DEVICE TYPE, PORE SIZE QTY. PART NO. 10mm Syringe Filters 0.20µm 50 2370 0.20µm 250 2365 25mm Syringe Filters 0.02µm 50 2132 PRICE Contact your Alltech office or distributor for current or local prices. F I LT R AT I O N Syringe Filters Mini- UniPrep™ Syringeless Filters Economy Syringe Filters • A cost effective alternative for general purpose use • Tabbed luer inlet fitting for stable connections • Available in 25mm diameter • Easy all-in-one filtration process • Fast preparation of HPLC samples 5903 Place your sample in the vial and compress the filter plunger to get a 12x32mm vial with your filtered sample, capped and ready to use! 4782 Color-coded polypropylene housings 25MM ECONOMY SYRINGE FILTERS PORE SIZE QTY. PART NO. 0.20µm 100 6837 0.45µm 100 6839 PTFE 0.20µm 100 6821 0.45µm 100 6823 Regenerated Cellulose 0.20µm 100 6829 0.45µm 100 6831 MEMBRANE Nylon 6760 Need Syringes? 5954 Compression tools make processing Mini-UniPreps fast and easy. See page 38 for disposable polypropylene or glass syringes. ™ MINI- UNIPREP™ SYRINGELESS FILTERS DESCRIPTION QTY. PART NO. Clear Mini-UniPrep™ 0.2µm Nylon 100 6806 0.45µm Nylon 100 6808 0.2µm PP 100 6817 0.45µm PP 100 6819 0.2µm PTFE 100 6814 0.45µm PTFE 100 6816 Amber Mini-UniPrep™ 0.2µm Nylon 100 6832 0.45µm Nylon 100 6834 0.2µm PP 100 6842 0.45µm PP 100 6844 0.2µm PTFE 100 6852 0.45µm PTFE 100 6854 6-Place Compression Tool ea 6866 1-Place Compression Tool ea 6861 © Copyright 2004 Alltech Associates, Inc. PRICE PRICE 4675 Contact your Alltech office or distributor for current or local prices. 35 F I LT R AT I O N Centrifuge Filter Tubes Micro-Spin® Centrifuge Filter Tubes Maxi-Spin™ Centrifuge Filter Tubes • Economical alternative to expensive, brand name filter tubes • For up to 850µL sample volume • Includes a glass support membrane • For up to 25mL sample volume • Use with fixed-angle rotor centrifuge • All tubes include a glass support membrane Maxi-Spin™ Centrifuge Filter Tubes remove particulates from larger sample sizes with any centrifuge that holds 50mL conical tubes. They should only be used with a fixed-angle rotor centrifuge to prevent premature clogging of the membrane. Maximum operating temperature: 50°C. Maximum G-force: 2500xG. Quickly and easily remove particulates and clarify small sample volumes. Less than 5µL hold-up volume provides near quantitative volumetric recovery. Maximum G-Force: 10,000xG. 4817 4784 ALLTECH MICRO-SPIN® FILTER TUBES MEMBRANE TYPE, PORE SIZE QTY. PART NO. Cellulose Acetate 0.20µm 100 24126 0.45µm 100 24133 Nylon 0.20µm 100 24137 0.45µm 100 24139 PVDF (Hydrophobic) 0.20µm 100 24142 0.45µm 100 24144 Regenerated Cellulose 0.20µm 100 24148 0.45µm 100 24150 PRICE MAXI-SPIN® FILTER TUBES MEMBRANE TYPE, PORE SIZE QTY. PART NO. Cellulose Acetate 0.20µm 50 24152 0.45µm 50 24154 Nylon 0.20µm 50 24156 0.45µm 50 24158 PVDF (Hydrophobic) 0.20µm 50 24160 0.45µm 50 24162 PRICE Forensic Spin Tubes • 600µL capacity • Extract biological fluids from cloth samples The 1.4mm mesh filter basket acts as a sieve to retain cloth samples and pass fluids to the receiver tube. The spin tube includes a 600µL filter basket and a 2mL microcentrifuge receiver tube with attached cap. Whatman Ultra-Spin Centrifuge Filter Tubes ™ • For up to 850µL sample volume • Operates by molecular weight cut-off WHATMAN ULTRA-SPIN® FILTER TUBES MEMBRANE TYPE, MW CUT-OFF QTY. PART NO. Ultrafilter 12kD, Cellulose Triacetate 100 2363 30kD, Polysulfone 100 2369 100kD, Polysulfone 100 2371 © Copyright 2004 Alltech Associates, Inc. 36 4818 PRICE MAXI-SPIN FILTER TUBES QTY. PART NO. 100 2566 250 2567 ® DESCRIPTION Forensic Spin Forensic Spin PRICE Contact your Alltech office or distributor for current or local prices. F I LT R AT I O N Filtration Membranes Alltech Pre-Cut Membranes • Variety of pore sizes and membrane types • Non-sterile ANODISC® PRE-CUT MEMBRANES DIAMETER PORE SIZE QTY. PART NO. 13mm (no support ring) 0.02µm 100 2935 13mm (no support ring) 0.10µm 100 2933 13mm (no support ring) 0.20µm 100 2253 25mm 0.02µm 50 2255 25mm 0.10µm 50 2268 25mm 0.20µm 50 2250 47mm 0.02µm 50 2235 47mm 0.10µm 50 2276 47mm 0.20µm 50 2230 4777 Anodisc® Membranes Anodisc® Membranes are hydrophilic and highly solvent resistant. They will work with both vacuum and pressure systems, although we recommend using a membrane support with vacuum systems. Use for general filtration and sterilization of aqueous and organic samples. 25mm and 47mm filters are bound to a polypropylene support ring. Cellulose Acetate Membranes Cellulose Acetate Membranes are hydrophilic and exhibit very low protein retention. Use for general biological filtration, sterilization, and filtration of aqueous solutions. Nylon Membranes Nylon Membranes are hydrophilic and resistant to many solvents Use for filtration and clarification of aqueous or organic solutions. DIAMETER 13mm 13mm 25mm 25mm 47mm 47mm CELLULOSE ACETATE PRE-CUT MEMBRANES PORE SIZE QTY. PART NO. 0.20µm 100 2125 0.45µm 100 2126 0.20µm 100 2128 0.45µm 100 2129 0.20µm 100 2133 0.45µm 100 2135 DIAMETER 13mm 13mm 25mm 25mm 47mm 47mm 90mm 90mm NYLON PRE-CUT MEMBRANES PORE SIZE QTY. PART NO. 0.20µm 100 2046 0.45µm 100 2044 0.20µm 100 2050 0.45µm 100 2048 0.20µm 100 2034 0.45µm 100 2024 0.20µm 25 2540 0.45µm 25 2546 DIAMETER 13mm 13mm 25mm 25mm 25mm 47mm 47mm 47mm PTFE PRE-CUT MEMBRANES PORE SIZE QTY. PART NO. 0.20µm 100 2013 0.45µm 100 2015 0.20µm 100 2023 0.45µm 100 2029 1.00µm 100 2056 0.20µm 100 2057 0.45µm 100 2058 1.00µm 100 2059 DIAMETER 47mm 47mm REGENERATED CELLULOSE PRE-CUT MEMBRANES PORE SIZE QTY. PART NO. 0.20µm 100 204700 0.45µm 100 504700 PTFE Membranes PTFE Membranes are hydrophobic and highly solvent resistant. Use for filtration of air, gases, and nonaqueous solutions. Pre-treat with methanol before using with aqueous solutions. Regenerated Cellulose Membranes PRICE PRICE PRICE PRICE PRICE Need Help Selecting the Correct Membrane? Regenerated Cellulose Membranes are hydrophilic and highly solvent resistant. Use for HPLC sample prep and general biological filtration. See pages 27-29 for individual membrane specifications and suggested use/applications. 4777 © Copyright 2004 Alltech Associates, Inc. Contact your Alltech office or distributor for current or local prices. 37 F I LT R AT I O N Filtration Accessories Personal Microcentrifuge Syringes • For general laboratory use • Uses luer hub needles – sold separately • Glass syringes with PTFE tipped plunger are gas and liquid tight. • Max. 6000 RPM, 2000xG force • Holds six 1.5mL or 2.0mL tubes This handy microcentrifuge fits right at your workstation. Comes with adapters for 0.5mL tubes. 3424 4675 SYRINGES DESCRIPTION QTY. PART NO. Glass/PTFE Syringes 1.0mL 1 81320 5.0mL 1 81520 Disposable Polypropylene Syringes with Luer Tip 1.0mL 12 44701100 5.0mL 12 447012 20mL 2 447018 PRICE Stainless Steel Syringe Filter Kit 4778 25mm Filter Syringe Kit Includes SS Filter Holder, 5mL Glass Syringe, O-Rings, and 50 Anodisc® Membranes Replacement Parts 13mm Stainless Steel Filter Holder 25mm Stainless Steel Filter Holder Replacement 13mm O-Rings Replacement 25mm O-Rings © Copyright 2004 Alltech Associates, Inc. 38 2060 2038 2042 20196 20198 PERSONAL MICROCENTRIFUGES PART NO. 22550 22551 PRICE Polypropylene Microcentrifuge Tubes • 17,000xG • 0.012" piercing port on caps For our full selection of syringes see pages 55-71. ALLTECH SYRINGE FILTER KITS DESCRIPTION PART NO. 13mm Filter Syringe Kit 2036 Includes SS Filter Holder, 1mL Glass Syringe, O-Rings, and 100 each of 0.2µm and 0.45µm Nylon Membranes DESCRIPTION 110V 220V, CE Certified PRICE POLYPROPYLENE MICROCENTRIFUGE TUBES DESCRIPTION COLOR QTY PART. NO. 0.5mL Standard (no graduations) Natural 1000 22301 Blue 22303 Green 22305 Red 22309 Yellow 22313 Amber 22315 1.5mL Standard (no graduations) Natural 500 22365 Blue 22367 Green 22369 Red 22373 Yellow 22377 Amber 22379 0.5mL Graduated Natural 1000 22302 Blue 22304 Green 22306 Red 22310 Yellow 22314 Amber 22316 1.5mL Graduated Natural 500 22385 Blue 22387 Green 22389 Red 22393 Yellow 22397 Amber 22399 2.0mL Graduated Natural 500 22405 Blue 22407 Green 22409 Red 22413 Yellow 22417 Amber 22419 PRICE Contact your Alltech office or distributor for current or local prices. F I LT R AT I O N Vacuum Filtration In-Line Vacuum Filtration Systems Filter Funnels and an In-Line Filter Cap share a common base to easily accommodate any vacuum filtration volume. In-Line Filter Cap Filter funnel has a threaded filter base that connects directly to any receiver with GL45 threads. It uses any 47mm diameter membrane. To use, connect the filter base directly to your vacuum source using one of the six tubing adapters included with each unit. Polypropylene construction is ideal for mobile phase filtration, degassing, and culture media filtration. Choose from 1L and 2L funnel volumes. Use the In-Line Filter Cap for 100mL to Multi-liter Filtration tasks. The In-Line system uses the threaded base of the funnel system but replaces the funnel with the in-line filter cap. Tubing is connected to the filter cap and draws liquid into the system from any container. This minimizes atmospheric exposure and handling hazards. The polypropylene fluid path uses any standard 47mm membrane. Alltech’s built-in priming valve restores flow in seconds without replacing the membrane or compromising the flow path. Base Use Vacuum Filter Funnels for 100mL to 2L Filtration tasks. Base Filter Funnel Filter Funnel 4391 In-Line Filter Cap 4385 VACUUM FILTRATION SYSTEMS DESCRIPTION QTY. PART NO. 1L Filter Funnel System 1 6409 Includes Base, Mesh Filter Support, Adapter Kit, 1L Funnel 2L Filter Funnel System Includes Base, Mesh Filter Support, Adapter Kit, 2L Funnel 1 6429 In-Line Filtration System Includes Base, Mesh Filter Support, Adapter Kit, In-Line Filter Cap 1 6407 1 1 1 1 1 6473 6463 6418 6438 6421 Replacement Parts 1L Funnel Only In-Line Filter Cap Only Replacement Mesh Filter Support Ring Replacement Adapter Kit Stainless Steel Mesh Filter Support Ring PRICE See Page 26 for Vacuum Pumps. SuperClean™ SPE Columns for Mobile Phase Clean-Up The Super-Clean SPE Column (Figure A) is a 75mL polypropylene column that contains a blend of carbon and glass pre-filters to remove particulates and dissolved organics in a single step. Add a syringe adapter and 1/8" o.d. tubing and connect to the In-Line Filtration System described above for large volume clean-up (Figure B) of mobile phases, buffers, and other high volume samples SUPERCLEAN™ SPE COLUMNS DESCRIPTION QTY. PART NO. SuperClean™ SPE Columns 16 29901 Syringe Adapters, 75mL 5 210709 1/8" o.d. PTFE Tubing 10' 20096 © Copyright 2004 Alltech Associates, Inc. PRICE 5950 Figure A Figure B One-step removal of particulates and organics increases HPLC column lifetime. 5953 Contact your Alltech office or distributor for current or local prices. 39
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