1. No category

Sample Requirements
Pathology Laboratory Guide
2012
Sample Requirements Edition 1
General Information
Sample Requirements
Sample requirements are listed showing the recommended collection tube.
In some instances alternative tubes may be used, eg the full range of tubes is
available for paediatric samples. Contact the relevant department to discuss:
Clinical Biochemistry ext: 5703 / 5 (01473 703703 / 5),
Haematology ext:
5709 (01473 703709) or
Microbiology ext:
5736 (01473 703736).
The volumes indicated are to identify the correct tube only and do not imply
that more than one Monovette is required for a selection of tests. Although if
clotted blood is required in more than one department please send separate
samples. If in doubt about minimum volumes for given assays, please contact
the relevant department to discuss.
Brown top White top Pink top Orange top Yellow top Green top Clotted sample
Clotted sample – no separating gel
EDTA
Lithium heparin
Fluoride
Citrate – must be filled to line or the sample will be rejected –
do not combine short samples to make one full tube
Blue top EDTA
Purple top Sedivette ESR tube – m
ust be filled to line or the sample will
be rejected
Red top EDTA
The minimum labelling criteria is surname plus two of the following: forename
or initial, date of birth, hospital number or NHS number.
For all blood transfusion samples the minimum criteria is full name (surname
and first name), hospital number or NHS number, date of birth, date and time of
collection and the sample must be signed by the person who took the sample.
Failure to meet these labelling criteria will result in the specimen being rejected.
Samples for Maternal Serum Screen must include either the hospital number or
NHS number as one of the three patient identifiers.
Samples for leukaemia immunophenotyping / cell markers (BCR / ABL, JAK2, PNH
Screen, EMA dye test and FISH), Genetics / Chromosomes (HFGE and HFE) and
tissue typing (HLA and HLA B27) must contain four points of reference, eg full
name (surname and first name), hospital number or NHS number and date of
birth.
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Pathology Laboratory Guide
General Information Sample Requirements Edition 1
Order of Draw for S-Monovette Blood Collection System
1
White
2
Brown
3
Green
4
Purple
5
Orange
6
Red
7
Blue
8
Yellow
Serum
Serum
Gel
Coagulation
ESR
Li Heparin
EDTA
EDTA
K Blood
Transfusion
Fluoride
Point of Care Testing
POCT coordinator: Alison Czarnota, bleep 917, Ext 5229 (01473 703229)
Ipswich Hospital users:
Blood glucose meters: For replacement meters, QC logbooks and batteries
please bring complete blood glucose meter box to Clinical Biochemistry. Lost
meters may incur a small charge. QC solutions are obtained from the Pharmacy
Department.
POCT equipment training: Training is available for blood gas analysers, urine
test strip meters, pregnancy test kits, Coaguchek XS plus INR meters, DCA2000
HbA1c analysers and urinary drugs of abuse screening kits. Please call the
POCT coordinator for details. Blood glucose meter training session details are
on the intranet.
e-Learning: Online competency assessments are available for many of the
POCT devices in use. Please call the POCT coordinator for details.
External Quality Assurance schemes (EQA): We are inspected under ISO 15189
and ISO 22870 which require all POCT medical devices be enrolled on EQA
schemes. Samples are sent regularly and participation by all staff that test
patient samples is mandatory. For details please contact the POCT coordinator.
Blood gas analysers: Emergency Department blood gas analyser is available
for use by trained staff only. For training and barcodes please contact the
POCT coordinator.
Blood gas samples should be collected into electrolyte balanced heparinised
syringes. Samples MUST be free from air bubbles, capped and mixed for at
least a minute before analysis. Samples not analysed immediately should be
mixed for longer.
New equipment: Any department considering introducing new POCT
equipment must seek the advice of the POCT committee. For details please
contact the POCT coordinator.
Community users:
EQA schemes, training and advice for POCT medical devices is available. Please
call the POCT coordinator on 01473 703229 or email
[email protected]
Pathology Laboratory Guide
3
Sample Requirements Edition 1
General Information
Haematology / Blood Transfusion & Clinical Biochemistry
Department of Haematology
Consultants:
Dr J A Ademokun
ext 5719 (01473 703719), bleep 160
Dr I M Chalmers
ext 5256 (01473 703256), bleep 125
Dr I N Whalley
ext 5256 (01473 703256), bleep 904
Head BMS Mr Nigel Brinkley
ext 5727 (01473 703727)
Secretary: ext 5718 (01473 703718)
fax 01473 703720
Haematology Enquiries:
ext 5709 / 10 (01473 703709 / 10)
Anticoagulant Monitoring Service Enquiries:
ext 5228 (01473 703228)
Blood Transfusion Enquiries:
ext 5726 (01473 703726)
Services:
Routine Haematology, Anticoagulant
Monitoring, Blood Transfusion and
Immunology.
Department of Clinical Biochemistry
Consultant:
Dr P Twomey
ext 5708 (01473 703708), bleep 910
Head BMS Mr Dean Braunig
ext 5704 (01473 703704)
Secretary: ext 5708 (01473 703708)
fax 01473 703259
Biochemistry Enquiries:
ext 5705 (01473 703705)
Services:
Routine Clinical Biochemistry and
Endocrinology.
Laboratory Hours
The laboratory operates a 24-hour service. Full routine and emergency services
operate between 9.00 am and 5.30 pm, Monday to Friday, with a partial service
to 1.00 am. Only emergency services operate between 1.00 am and 9.00 am and
at weekends.
Urgent Requests
Urgent requests requiring immediate analysis, all blood gases and all
crossmatch requests must be notified by telephone to the department or, after
5.30 pm, by bleep 905 for Haematology and bleep 906 for Biochemistry.
All Emergency Department (A&E) requests where there is a likelihood of
4 hour breach must be notified by telephone to the relevant department.
Semi-urgent Requests
Samples received in the department between 5.30 pm and 1.00 am will be
analysed and the results available electronically within 2 hours of receipt.
There is no need to bleep for each request.
All requests from 1.00 am to 9.00 am must be notified by bleep 905 for
Haematology and bleep 906 for Biochemistry, unless the results are not
required within 2 hours.
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Pathology Laboratory Guide
General Information Sample Requirements Edition 1
Blood samples should be sent via the air tube, station 5709 for Haematology,
station 5705 for Biochemistry. CSF must be delivered by hand.
Web Browser
The introduction of a web browser within the hospital gives access to all
pathology results, even to GP-requested work. The web browser should be
available to all satellite and other hospitals in the future.
A full audit record of all enquiries including enquirer ID is logged automatically.
Any abuse of this system will be dealt with under the Trust disciplinary policy.
For details of access to the web browser contact Mr Andrew Block, Pathology
Computer Manager on 01473 704403 or extension 6403 or alternatively, Miss
Marisa Van Anson on 01473 703721 or extension 5721.
Collection of Fasting Samples
Fasting for plasma glucose is a minimum of 8 hours.
For lipids it is a minimum of 12 hours.
Anticoagulant Monitoring Service (AMS)
The Anticoagulant Monitoring Service must receive an AMS referral for both
new and existing anticoagulant patients on discharge from hospital.
To refer inpatients use Lorenzo iCM (select patient from patient list, enter
order and then select ACR or AMSR).
To refer outpatients use an AMS referral form or its PDF equivalent and send
by post or fax to 01473 703720. New outpatients can make appointments to
see the anticoagulant nurse specialist.
Please note that underfilled INR samples cannot be tested and the AMS will
request a repeat sample.
Laboratory Phlebotomist Opening Hours:
The laboratory open hours are Monday – Friday 7.30 am – 5.45 pm.
Saturday phlebotomy by strict appointment only, 9.00 am – 11.30 am,
telephone 01473 703721 for appointments.
Phlebotomy Services to Wards
Ward Rounds: Weekdays
8.00 am – 12.00 noon
Saturdays (limited) 7.30 am – 10.30 am
Sundays (limited)
7.30 am – 10.00 am
The weekend phlebotomy service is for clinically essential requests only.
This guide is by no means comprehensive. If in doubt, please refer to the
comprehensive Laboratory Manual available on the hospital intranet at
Business Units Ô Clinical Support Services and Cancer Ô Pathology Ô
Pathology Handbook. If you are still in doubt, please telephone for advice.
Pathology Laboratory Guide
5
Sample Requirements Edition 1
General Information
Microbiology
Laboratory hours:
Monday to Friday: 9.00 am – 5.30 pm
Saturday: 9.00 am – 12.30 pm
General enquiries:
ext 5736 (01473 703736)
Clinical interpretation:Dr Richard Kent ext 5741 (01473 703741)
Dr S Ginwalla ext 5745 (01473 703745)
Head BMS Andrew Bendall ext 5747 (01473 703747)
The department welcomes enquiries. Please do not hesitate to contact us for
information, advice or visits.
Urgent samples
• Must be arranged by telephone both during the day and on-call
• Results will be telephoned
• Samples / Tests included:
–CSF
– Sputum / NBL
– NPA for RSV (children) tested as one batch out-of-hours only
–Urine
– Wound swabs / tissue / fluids
Note: G
ent / Vanc assays performed by Clinical Biochemistry
• Other tests after discussion with microbiology consultant.
• Medical advice is always available – contact switchboard if out-ofhours.
On-call Service (not resident)
Monday to Friday: 5.30 pm – 9.00 am
Saturday:
12.30 pm – 9.00 am
Sunday:
9.00 am – 9.00 am
Bleep 907 or contact switchboard for on-call staff if no reply. After midnight
on-call restricted to CSFs. Other samples by arrangement between patient’s
consultant and microbiology consultant only.
OCM Ward Ordering
• One sample per request please.
• Completed results are available on microbiology screen.
• Urgent interim results are phoned.
Transport
All samples must be labelled and accompanied with a completed request form
with brief relevant clinical details.
Samples, except blood cultures and non-repeatable samples eg CSF, may be
sent by air tube to Microbiology.
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Pathology Laboratory Guide
General Information Sample Requirements Edition 1
Out-of-hours blood cultures should be placed in the incubator in the blood
bank room immediately after collection. All significant blood culture results
are phoned.
Non-repeatable samples should be delivered by hand to the laboratory.
All samples which are thought to contain high risk pathogens eg HIV, Hep B / C
and M tuberculosis must be labelled ‘Danger of Infection’ together with the
request form.
Pathology Laboratory Guide
7
Sample Requirements Edition 1
General Information
Histology and Cytology Specimen Requirements
Routine Histology – Please refer to Pathology Handbook for
comprehensive information. (Business Units Ô Clinical Support Services and
Cancer Ô Pathology Ô Pathology Handbook)
Routine histology specimens must be sent in formalin fixative in a container
appropriate to the size of the sample.
Frozen Sections
Frozen sections should be booked before the surgery is planned, this ensures
the relevant staff are available to process and analyse the sample. The sample
should be sent unfixed and handed to a member of the Histology staff.
Muscle Biopsies
Sample collection kit available, by request, from the Histology department.
Please allow 24 hours for preparation.
Nerve Biopsies
Sample collection kit available, by request, from the Histology department.
Please allow 24 hours for preparation.
Cytology
Gynae Cytology: Use Cytyc liquid-based cytology vial.
Non-gynae Cytology: Unfixed samples in a named universal with the exception
of urine samples that must be collected in a urine cytology container available
from the laboratory.
Post Vasectomy Semen Samples: Use semen analysis pack.
Semen Samples for Fertility Assessment:
Please ensure patient receives semen analysis pack which contains:
• Patient information leaflet
• Request form
• Sample collection pot
• Patient questionnaire
8
Pathology Laboratory Guide
Clinical Biochemistry Sample Requirements Edition 1
Clinical Biochemistry Department
Tel: 5703 / 5 (01473 703703 or 703705)
Profiles:
U/E (E) [Urea, Na, K and creatinine]
Blood 7.5 ml Brown Top
LFT (L) [ALP, TP, Alb, Glob, ALT and bilirubin]
Blood 7.5 ml Brown Top
Bone (B) [Ca, PO4, ALP, TP, Alb and Glob]
Blood 7.5 ml Brown Top
Lipids (LIP) [Cholesterol, Triglycerides and HDL]
Blood 7.5 ml Brown Top
TFT (T) [FT4 and TSH]
Blood 7.5 ml Brown Top
TEST
SAMPLE
Acyl Carnitine
2 blood spots (Guthrie Card)
Adrenocorticotrophic Hormone (ACTH)
Blood 2 x 3.4 ml Pink Top
Send to laboratory immediately on ice.
Alanine Transaminase (ALT)
Blood 7.5 ml Brown Top
Alcohol (Ethanol)
Blood 7.5 ml Brown Top
Postmortem / clinical samples only.
Albumin (ALB)
Blood 7.5 ml Brown Top
Aldosterone
Blood 3.4 ml Pink Top.
Separate EDTA (Pink Top) sample
required for this assay.
Usually performed with Renin assay.
Alkaline Phosphatase (ALP)
Blood 7.5 ml Brown Top
ALP Electrophoresis (ALPE)
Blood 7.5 ml Brown Top
Alpha-1 Antitrypsin (A1AT)
Blood 7.5 ml Brown Top
Alpha Foetoprotein (AFP)
Blood 7.5 ml Brown Top
Aluminium (AL)
Blood 9.0 ml. Special tube required.
Contact laboratory.
Amiodarone (AMIO)
Blood 7.5 ml Brown Top
Amino Acids (AA)
Blood 2.0 ml Orange Top
Ammonia (AMM)
Blood 2.0 ml Orange Top.
Contact laboratory BEFORE sample
collection and transfer sample to
laboratory within 30 mins on ice.
Amylase (AMY)
Blood 7.5 ml Brown Top
Androstenedione (AND)
Blood 7.5 ml Brown Top
Angiotensin Converting Enzyme (ACE)
Blood 7.5 ml Brown Top
Pathology Laboratory Guide
9
Sample Requirements Edition 1
Clinical Biochemistry
Anion Gap
Blood 7.5 ml Brown Top
Aspartate Transaminase (AST)
Blood 7.5 ml Brown Top
Anti-Mullerian Hormone (AMH)
Blood 7.5 ml Brown Top
Send to laboratory immediately.
Aspirin (Salicylate)
Blood 7.5 ml Brown Top
B12
Blood 7.5 ml Brown Top
Beta-2 Microglobulin (B2M)
Blood 7.5 ml Brown Top
BHCG
Blood 7.5 ml Brown Top
Bicarbonate (Serum) (CO2)
Blood 7.5 ml Brown Top
Bile Acids (BILE)
Blood 7.5 ml Brown Top
Bilirubin (Conjugated) (BIC)
Blood 7.5 ml Brown Top
Bilirubin (Total) (BIT)
Blood 7.5 ml Brown Top
Bilirubin (Unconjugated) (BIU)
Blood 7.5 ml Brown Top
Bismuth
Blood 3.4 ml Pink Top
Separate EDTA (Pink Top) sample
required for this assay.
Blood Gas
Collect 0.3 ml of arterial blood into
commercial syringe or capillary with
no air bubbles present. Blood gases
must only be collected by trained
personnel. Blood gas samples can be
sent to the laboratory via the air tube
system.
Bone Alkaline Phosphatase (ALPB)
Blood 7.5 ml Brown Top
C2H5OH
Blood 7.5 ml Brown Top
CA12-5
Blood 7.5 ml Brown Top
CA15-3
Blood 7.5 ml Brown Top
CA19-9
Blood 7.5 ml Brown Top
Cadmium
Blood 3.4 ml Pink Top
Separate EDTA (Pink Top) sample
required for this assay.
Caeruloplasmin (CP)
Blood 7.5 ml Brown Top
Calcitonin
Blood 7.5 ml Brown Top
Send to lab immediately on ice.
Calcium (CA)
Blood 7.5 ml Brown Top
Calcium (adjusted for albumin)
Blood 7.5 ml Brown Top
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Pathology Laboratory Guide
Clinical Biochemistry Sample Requirements Edition 1
Carbamazepine (CAR) [CBZ]
Blood 7.5 ml Brown Top
(immediately prior to dose)
Carbon Dioxide Serum (CO2)
Blood 7.5 ml Brown Top
Carboxyhaemoglobin (COHB)
Blood 9.0 ml Orange Top
Carcino-embryonic Antigen (CEA)
Blood 7.5 ml Brown Top
Carotene
Blood 7.5 ml Brown Top
Protect from light.
Chloride (CL)
Blood 7.5 ml Brown Top
Cholesterol (CH)
Blood 7.5 ml Brown Top
Cholesterol (HDL)
Blood 7.5 ml Brown Top
Cholesterol (LDL)
Blood 7.5 ml Brown Top
Cholinesterase
Blood 3.4 ml Pink Top
Separate EDTA (Pink Top) sample
required for this assay.
(organo-phosphate poisoning)
Cholinesterase Phenotype
Blood 7.5 ml Brown Top
(dibucaine number etc)
Chromium
Blood 9.0 ml. Special tube required
Contact laboratory.
Chromogranin A
Contact laboratory before collection.
Chromogranin B
Contact laboratory before collection.
CK (Total)
Blood 7.5 ml Brown Top
CO2 (Serum)
Blood 7.5 ml Brown Top
Cobalt
Blood 9.0 ml. Special tube required
Contact laboratory.
Copper (CU)
Blood 7.5 ml Brown Top
Cortisol (COR)
Blood 7.5 ml Brown Top
Must state time of collection.
C Peptide
Blood 7.5 ml Brown Top
Send to laboratory immediately.
C Reactive Protein (CRP)
Blood 7.5 ml Brown Top
Creatine Kinase (CK)
Blood 7.5 ml Brown Top
Creatinine (CR)
Blood 7.5 ml Brown Top
Cryoglobulin (CRY)
Blood 7.5 ml Brown Top
Deliver to laboratory immediately.
Pathology Laboratory Guide
11
Sample Requirements Edition 1
Clinical Biochemistry
Cyclosporin (CYC)
Blood 3.4 ml Pink Top
Separate EDTA (Pink Top) sample
required for this assay.
1-25 Dihydroxy Vitamin D (1-25-di-OH
cholecalciferol) [125D]
Blood 7.5 ml Brown Top
11 Deoxycortisol
Contact laboratory.
Dehydroepiandrosterone Sulphate (DHEAS)
Blood 7.5 ml Brown Top
Dibucaine
Blood 7.5 ml Brown Top
Digoxin (DIG)
Blood 7.5 ml Brown Top
collected 6 hr post dose.
Disopyramide
Blood 7.5 ml Brown Top
E2 (Oestradiol)
Blood 7.5 ml Brown Top
Ethosuximide
Blood 7.5 ml Brown Top
Fat Soluble Vitamins (FSV)
Blood 7.5 ml Brown Top
Ferritin
Blood 7.5 ml Brown Top
FK 506 (Tacrolimus) [TAC]
Blood 3.4 ml Pink Top
Separate EDTA (Pink Top) sample
required for this assay.
Flecainide
Blood 7.5 ml Brown Top
Folate
Blood 7.5 ml Brown Top
Follicle Stimulating Hormone (FSH)
Blood 7.5 ml Brown Top
State date of last menstrual period
if known.
Free Androgen Index (FAI)
Blood 7.5 ml Brown Top
Free Light Chains (FLC)
Blood 7.5 ml Brown Top
Free T3 (FT3)
Blood 7.5 ml Brown Top
Free T4 (T)
Blood 7.5 ml Brown Top
Galactosidase
Blood 3.4 ml Pink Top
Send to laboratory before 12 noon
Mon – Weds only.
Gamma GT (GGT)
Blood 7.5 ml Brown Top
Gamma Glutamyl Transferase (GGT)
Blood 7.5 ml Brown Top
Gastrin
Contact laboratory BEFORE
sample collection.
Gentamicin
Blood 7.5 ml Brown Top
Record time of collection on request form.
12
Pathology Laboratory Guide
Clinical Biochemistry Sample Requirements Edition 1
GFR
Blood 7.5 ml Brown Top
Globulin (GLOB)
Blood 7.5 ml Brown Top
Glucagon
Contact laboratory BEFORE
sample collection.
Glucose (G)
Blood 2.7 ml Yellow Top
Glucose Tolerance Test (GTT)
Contact laboratory.
Glycosylated Hb (HbA1c)
Blood 3.4 ml Pink Top
Separate EDTA (Pink Top) sample
required for this assay.
Growth Hormone (GH)
Blood 7.5 ml Brown Top
Gut Hormones
Contact laboratory BEFORE
sample collection.
Haematinics
Blood 7.5 ml Brown Top
Haemoglobin A1c (HbA1c)
Blood 3.4 ml Pink Top
Separate EDTA (Pink Top) sample
required for this assay.
Haptoglobin (HAP)
Blood 7.5 ml Brown Top
βHCG (Tumour Marker) [HCGB]
Blood 7.5 ml Brown Top
Hexosaminidase
Blood 3.4 ml Pink Top
Send to laboratory before 12 noon
Mon – Weds only.
Homocysteine (Total) –
for investigation of arteriosclerosis risk
Blood 9.0 ml Orange Top
Send to laboratory immediately.
17 Hydroxyprogesterone (OHP)
Blood 7.5 ml Brown Top
Immunoglobulin A (IgA)
Blood 7.5 ml Brown Top
Immunoglobulin G (IgG)
Blood 7.5 ml Brown Top
Immunoglobulin G
(Subclasses 1,2,3,4) [IgGSub]
Blood 7.5 ml Brown Top
Immunoglobulin M (IgM)
Blood 7.5 ml Brown Top
Immunoglobulins (IgS)
Blood 7.5 ml Brown Top
Inhibin A & B
Blood 7.5 ml Brown Top
Insulin
Blood 7.5 ml Brown Top and Blood
2.7 ml Yellow Top for glucose assay.
Send to laboratory immediately.
Insulin-like Growth Factor 1 (IGF1)
Blood 7.5 ml Brown Top
Iron
Blood 7.5 ml Brown Top
Pathology Laboratory Guide
13
Sample Requirements Edition 1
Clinical Biochemistry
Lactate
Blood 2.7 ml Yellow Top
Lactate Dehydrogenase (LD)
Blood 7.5 ml Brown Top
Lamotrigine
Blood 7.5 ml Brown Top
Lead (PB)
Blood 3.4 ml Pink Top
Separate EDTA (Pink Top) sample
required for this assay.
Lipase
Blood 7.5 ml Brown Top
Lutenising Hormone (LH)
Blood 7.5 ml Brown Top
State date of last menstrual period
if known.
Lithium (LI)
Blood 7.5 ml Brown Top
Magnesium (MG)
Blood 7.5 ml Brown Top
Maternal Serum Screen
Blood 7.5ml Brown Top
Samples must include either the
hospital number or NHS number as one
of the three patient identifiers.
Methaemalbumin
Blood 7.5 ml Orange Top
or 3.4 ml Pink Top freshly collected
Mercury (Blood)
Contact laboratory
Methaemoglobin
Blood 4.5 ml Orange Top
Micronutrient Screen
Blood 9.0.ml Orange Top *2
and Blood 7.5 ml Brown Top *2
Protect from light.
Myeloma Screen
Blood 7.5 ml Brown Top
Neurotensin
Contact laboratory BEFORE
sample collection.
Oestradiol (17 Beta) [E2]
Blood 7.5 ml Brown Top
Osmolality (OSM)
Blood 7.5 ml Brown Top
Pancreatic Polypeptide
Contact laboratory BEFORE
sample collection.
Paracetamol (PAR)
Blood 7.5 ml Brown Top collected
> 4 hr post ingestion.
Parathormone (PTH)
Blood 3.4 ml Pink Top
Send to laboratory within 8 hours
together with 7.5 ml Brown Top.
Paraprotein (PP)
Blood 7.5 ml Brown Top
Phenobarbitone (PHB)
Blood 7.5 ml Brown Top
– collect any time
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Pathology Laboratory Guide
Clinical Biochemistry Sample Requirements Edition 1
Phenytoin (PHE)
Blood 7.5 ml Brown Top
– collect any time
Phosphate (PO4)
Blood 7.5 ml Brown Top
Phytanic Acid
Blood 3.4 ml Pink Top
Separate EDTA (Pink Top) sample
required for this assay.
Plasma Potassium (KP)
Blood 7.5 ml Orange Top
Porphyrins
For screening for porphyria send
random urine, random faeces &
EDTA blood at the same time.
Protect from light.
Potassium (K)
Blood 7.5 ml Brown Top
Potassium (Plasma)
Blood 7.5 ml Orange Top
Pro BNP
Blood 7.5 ml Brown Top
Procollagen Extension Peptide (P1NP)
Blood 4.5 ml Orange Top
Procollagen IIIP (PCP3) [PIIINP]
Blood 9.0 ml White Top Non-gel tube
Progesterone (PRG)
Blood 7.5 ml Brown Top
State date of last menstrual period
if known.
Prolactin (PRL)
Blood 7.5 ml Brown Top
Prostate Specific Antigen (PSA)
Blood 7.5 ml Brown Top
Protein (P)
Blood 7.5 ml Brown Top
Protein Electrophoresis (PE)
Blood 7.5 ml Brown Top
PTH
Renin
Blood 3.4 ml Pink Top
Send to laboratory within 8 hours
together with 7.5 ml Brown Top.
Blood 3.4 ml Pink Top
Separate EDTA (Pink Top) sample
required for this assay. Usually
performed with Aldosterone assay.
Salicylate
Blood 7.5 ml Brown Top
Sex Hormone Binding Globulin (SHBG)
Blood 7.5 ml Brown Top
Sirolimus (Rapamycin) [SIR]
Blood 3.4 ml Pink Top
Separate EDTA (Pink Top) sample
required for this assay.
Sodium (NA)
Blood 7.5 ml Brown Top
Tacrolimus (FK506) [TAC]
Blood 3.4 ml Pink Top
Separate EDTA (Pink Top) sample
required for this assay.
Pathology Laboratory Guide
15
Sample Requirements Edition 1
Clinical Biochemistry
Testosterone (TES)
Blood 7.5 ml Brown Top
Theophylline (THE)
Blood 7.5 ml Brown Top
– collect prior to dose
Thio Purine Methyl Transferase (TPMT)
Blood 3.4 ml Pink Top
Separate EDTA (Pink Top) sample
required for this assay. TPMT assay must
now be received in the laboratory on
Monday – Wednesday only.
Thyroglobulin
Blood 7.5 ml Brown Top
Thyroid Peroxidase Antibodies (TPO)
Blood 7.5 ml Brown Top
Thyroid Stimulating Hormone (TSH)
Blood 7.5 ml Brown Top
Thyroxine (Free) [FT4]
Blood 7.5 ml Brown Top
Total CO2 (Serum) [CO2]
Blood 7.5 ml Brown Top
Transferrin
Blood 7.5 ml Brown Top
Triglyceride (TG)
Blood 7.5 ml Brown Top
Fasting Only.
Triiodothyronine (Total T3) [T3]
Blood 7.5 ml Brown Top
Troponin I (TNI)
Blood 7.5 ml Brown Top
Tryptase
Blood 3.4 ml Pink Top
Contact laboratory BEFORE sample
collection.
TSH Receptor Antibody (TRAB)
Blood 7.5 ml Brown Top
Urea (UR)
Blood 7.5 ml Brown Top
Uric Acid (UA)
Blood 7.5 ml Brown Top
Valproate
Blood 7.5 ml Brown Top
– collect any time
Vancomycin
Blood 7.5 ml Brown Top
Record time of collection on request
form.
Vasoactive Intestinal Peptide (VIP)
Contact laboratory BEFORE
sample collection.
Very Long Chain Fatty Acids (VLCFA)
Blood 3.4 ml Pink Top
Separate EDTA (Pink Top) sample
required for this assay.
Vitamin A
Blood 7.5 ml Brown Top
Protect from light.
Vitamin B1 (Thiamine)
Blood 9.0 ml Orange Top
Vitamin B6 (Pyridoxine)
Blood 9.0 ml Orange Top
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Pathology Laboratory Guide
Clinical Biochemistry Sample Requirements Edition 1
Vitamin B Screen
2 x Blood 9.0 ml Orange Top
Vitamin D (25 Hydroxy Cholecalciferol)
Blood 7.5 ml Brown Top
Vitamin E
Blood 7.5 ml Brown Top
– protect from light
White Cell Enzymes (WCE)
2 x Blood 3.4 ml Pink Top
Send to laboratory before 12 noon
Mon – Weds only.
Zinc (ZN)
Blood 7.5 ml Brown Top
Urine Specimens – collection and preservation
Test
Collection
Preservative Notes
Amino acids
Random
Nil
Amylase
24 hr
Nil
Bence-Jones Protein
Random
Nil
Bile Pigments / Salts
Random
Nil
Calcium
24 hr
Hydrochloric acid
Send to laboratory
immediately.
Catecholamines
(CATD)
24 hr
Nil
Not routinely
available,
Metanephrine
assay is more
sensitive.
Please contact the
laboratory if
catecholamines
required.
Citrate
24 hr
Hydrochloric acid
Unsuitable for
preservation on
receipt.
Copper
Random or 24 hr
Nil
Cortisol
24 hr
Nil
Creatinine
24 hr
Nil
Qualitative Cystine
Random or 24 hr
Nil
Pathology Laboratory Guide
Acidified collection
acceptable.
17
Sample Requirements Edition 1
Quantitative Cystine
Random or 24 hr
Nil
Drugs of abuse
Random
Nil
Electrolytes (Na / K)
Random or 24 hr
Nil
Glucose
Random
Nil
Glycosaminoglycans
Random
Nil
Haemoglobin
Random
Nil
Clinical Biochemistry
Send to laboratory
immediately.
5HIAA – quantitative
24 hr
Glacial acetic acid
Unsuitable for
preservation on
receipt. See dietary
restrictions.
Hydroxyproline
24 hr
Nil
Send to laboratory
immediately.
Magnesium
24 hr
Hydrochloric acid
Melanin
Random
Nil
Mercury
Random or 24 hr
Nil
Send to laboratory
immediately.
Metanephrine
24 hr
Glacial acetic acid
Unsuitable for
preservation on
receipt. See dietary
restrictions.
Methylmalonate
Creatinine Ratio
Random
Nil
Send to laboratory
immediately.
Microalbumin
Random
Nil
Mucopolysaccharides Random
Nil
Myoglobin
Random
Nil
N-Telopeptide Xlinks
Random
Nil
Organic acids
Random
Nil
Osmolality
Random
Nil
Oxalate
24 hr
Hydrochloric acid
PH
Random
Nil
Phosphate
24 hr
Hydrochloric acid
Porphobilinogen
Random
Nil
18
Send to laboratory
immediately.
Send to laboratory
immediately.
Unsuitable for
preservation on
receipt.
Protect from light.
Pathology Laboratory Guide
Clinical Biochemistry Sample Requirements Edition 1
Porphyrin – screen
Random
Nil
Protect from light.
Porphyrins – 24 hr
24 hr
Nil
Protect from light.
Protein and / or
electrophoresis
Random or 24 hr
Nil
Reducing substances
Random
Nil
Steroid profile
24 hr
Nil
Sulphonylurea
Random
Nil
Total protein /
Creatinine ratio
Random
Nil
Urate
24 hr
Nil
Urea
Random or 24 hr
Nil
Urobilin
Random
Nil
VMA – Paediatric
requests only (< 18
years old). Adults
change to
Metanephrine
(METD).
24 hr
Hydrochloric acid
Unsuitable for
preservation on
receipt.
VMA
Fresh random
urine less than 4
hours old on
paediatric samples
only (< 18 years
old)
Nil
Send to laboratory
immediately.
Acidified collection
NOT acceptable.
Dietary Restrictions
Indoles are present in a number of foods and can give a false positive 5HIAA.
Suggest repeat urine collection avoiding the following foods for four days
before the collection and during the collection: bananas, avocados, red plums,
aubergines, pineapples, tomatoes, walnuts, blackcurrants, redcurrants and
chocolate.
Foodstuffs rich in capsaicin causes interference with the metanephrine assay so
must be avoided for four days before collection starts, and during collection.
These include: chilli peppers, chilli powder, curry powder and capsaicin paste.
If a test is not listed above, please contact the laboratory before starting the
collection.
If a combination of tests is requested requiring different preservatives, please
contact the laboratory for advice.
Pathology Laboratory Guide
19
Haematology
Sample Requirements Edition 1
Haematology Department – Tel: 5709 (01473 703709)
TEST
SAMPLE
Acanthocytes
Blood 3.4 ml Pink Top
ACRA (Acetyl Choline Receptor Antibody)
Blood 7.5 ml Brown Top
Activated Protein C Resistance
See prothrombotic screen.
Adrenal Antibodies
Blood 7.5 ml Brown Top
ANA
Blood 7.5 ml Brown Top
ANCA
Blood 7.5 ml Brown Top
ANCA MPO
Blood 7.5 ml Brown Top
ANCA PR3
Blood 7.5 ml Brown Top
Antenatal Blood Group Serology
Blood 4.5 ml Blue Top
Antenatal Haemoglobinopathy Screen (FOQ)
Blood 3.4 ml Pink Top
Antenatal Infectious Disease Serology
Blood 7.5 ml Red Top
Anti B2 glycoprotein Ab
Blood 7.5 ml Red Top
Anti Cardiolipin
Blood 7.5 ml Brown Top
Anti CCP Antibodies
Blood 7.5 ml Brown Top
Anti Choline Receptor Antibodies
Blood 7.5 ml Brown Top
Anti Endomysial Antibodies
Blood 7.5 ml Brown Top
Anti FXa assay
See Heparin assay
Anti GBM
Blood 7.5 ml Brown Top
Anti GPC
Blood 7.5 ml Brown Top
Anti LKM
Blood 7.5 ml Brown Top
Anti M2
Blood 7.5 ml Brown Top
Anti Mitochondrial
Blood 7.5 ml Brown Top
Anti Neuromyelitis Optica Antibodies
Blood 7.5 ml Brown Top
Anti Neuronal antibodies
Blood 7.5 ml Brown Top
Anti Neutrophil Cytoplasmic Antibodies
Blood 7.5 ml Brown Top
Anti NMDA Receptor Antibodies
Blood 7.5 ml Brown Top
Anti Nuclear Antibodies
Blood 7.5 ml Brown Top
Anti Nuclear HEp-2 Antibodies
Blood 7.5 ml Brown Top
Anti PDH
Blood 7.5 ml Brown Top
20
Pathology Laboratory Guide
Haematology Sample Requirements Edition 1
Anti Pemphigoid Antibodies
Blood 7.5 ml Brown Top
Anti Pemphigus Antibodies
Blood 7.5 ml Brown Top
Anti Phospholipid Antibodies
Blood 7.5 ml Brown Top +
2 x 3.0 ml Green Top
Anti Smooth Muscle
Blood 7.5 ml Brown Top
Anti TTG
Blood 7.5 ml Brown Top
Antibody Screen (Immunology / Haematology) Blood 7.5 ml Brown Top
Antibody Screen (Transfusion)
Blood 4.5 ml Blue Top – see blood
transfusion labelling criteria inside
front cover.
Antithrombin
See prothrombotic screen.
APCR
See prothrombotic screen.
APTT
Blood 3.0 ml Green Top
APTT Ratio
Blood 3.0 ml Green Top
Autoantibodies
Blood 7.5 ml Brown Top
Auto Immune Antibodies
Blood 7.5 ml Brown Top
Basement Membrane Antibody
Blood 7.5 ml Brown Top
B Cell Sub Sets
2 x Blood 3.4 ml Pink Top
Deliver to Haematology by 1 pm.
Basophils
See Full Blood Count.
BCR / ABL
5 x Blood 3.4 ml Pink Top
Beta 2 glycoprotein (anti b2gp)
Blood 7.5 ml Brown Top
Blood Group
Blood 4.5 ml Blue Top
C1 Esterase
Blood 7.5 ml Brown Top
C1 Inactivator
Blood 7.5 ml Brown Top
C3C4
Blood 7.5 ml Brown Top
Cardiolipin Antibodies
Blood 7.5 ml Brown Top
CBC
See Full Blood Count.
CCP Antibodies
Blood 7.5 ml Brown Top
CD3 / CD4 / CD8
See T cell Subsets.
Cell Markers
3 x Blood 3.4 ml Pink Top
Deliver to Haematology by 1 pm.
Centromere Antibody
See ENA Antibody Screen.
Pathology Laboratory Guide
21
Haematology
Sample Requirements Edition 1
Chromosomes
Blood 4.5 ml Orange Top
Do not take after 12 noon Friday.
Clotting Screen
Blood 3.0 ml Green Top
Coeliac Screen (TTG)
Blood 7.5 ml Brown Top
Cold Agglutinins
Blood 4.5 ml Blue Top – keep sample
warm and deliver to Blood Transfusion
immediately. See blood transfusion
labelling criteria inside front cover.
Complement C1 Inhibitor
Blood 7.5 ml Brown Top
Complement C3
Blood 7.5 ml Brown Top
Complement C4
Blood 7.5 ml Brown Top
Complement CH50
Blood 7.5 ml Brown Top
Coombs Test
Blood 3.4 ml Pink Top
or 4.5 ml Blue Top
Crithidia Luciliae Antibodies
Blood 7.5 ml Brown Top
Crossmatch
Blood 4.5 ml Blue Top – see blood
transfusion labelling criteria inside
front cover.
Cystic Fibrosis Screen
2 x Blood 3.4 ml Pink Top
DCT
Blood 3.4 ml Pink Top
or 4.5 ml Blue Top
D-Dimer
Blood 3.0 ml Green Top
Differential Count
See Full Blood Count.
Differential Leukocyte Count (DLC)
See Full Blood Count.
DNA Antibodies
Blood 7.5 ml Brown Top
DNA Studies for Gene Analysis
3 x Blood 3.4 ml Pink Top
DRVVT
See prothrombotic screen.
ELA2 Mutations
2 x Blood 3.4 ml Pink Top
EMA Dye Test for Hereditary Spherocytosis
Blood 3.4 ml Pink Top
ENA Antibody Screen
Blood 7.5 ml Brown Top
End Plate Antibodies
Blood 7.5 ml Brown Top
Eosinophils
See Full Blood Count.
EPO
Blood 7.5 ml Brown Top
Erythropoetin Antibody
Blood 7.5 ml Brown Top
Erythropoetin Assay
Blood 7.5 ml Brown Top
ESR
Blood 3.4 ml Pink Top
22
Pathology Laboratory Guide
Haematology Sample Requirements Edition 1
Exon 12
5 x Blood 3.4 ml Pink Top
Factor II (Factor II:C)
2 x Blood 3.0 ml Green Top
Factor II (G20210A) mutation
Blood 3.4 ml Pink Top
Factor IX (Factor IX:C)
2 x Blood 3.0 ml Green Top
Factor V (Factor V:C)
2 x Blood 3.0 ml Green Top
Factor V Leiden
See prothrombotic screen.
Factor VII (Factor VII:C)
2 x Blood 3.0 ml Green Top
Factor VIII (Factor VIII:C)
2 x Blood 3.0 ml Green Top
Factor VIII Inhibitor
3 x Blood 3.0 ml Green Top
Factor VIII Profile
3 x Blood 3.0 ml Green Top
Factor VIII Related Antigen
3 x Blood 3.0 ml Green Top
Factor X (Factor X:C)
2 x Blood 3.0 ml Green Top
Factor XI (Factor XI:C)
2 x Blood 3.0 ml Green Top
Factor XII (Factor XII:C)
2 x Blood 3.0 ml Green Top
Factor XIII Screen
4 x Blood 3.0 ml Green Top
FBC
Blood 3.4 ml Pink Top
Fibrinogen
Blood 3.0 ml Green Top
Fluorescent In Situ Hybridisation (FISH)
Fragile X Chromosome
Blood 3.4 ml Pink Top or
Blood 9.0ml Orange Top
Blood 4.5 ml Orange Top +
Blood 3.4 ml Pink Top
Free Protein S
See prothrombotic screen.
Full Blood Count
Blood 3.4 ml Pink Top
G6PD
Blood 3.4 ml Pink Top
GAD Antibodies
Blood 7.5 ml Brown Top
Gastric Parietal Cell Antibody
Blood 7.5 ml Brown Top
GBM Antibodies
Blood 7.5 ml Brown Top
GF
Blood 3.4 ml Pink Top
Glandular Fever Screen
Blood 3.4 ml Pink Top
Gliaden Antibodies
Blood 7.5 ml Brown Top
GM1 (ganglioside) Antibodies
Blood 7.5 ml Brown Top
Granulocyte Immunology
Contact Haematology
Pathology Laboratory Guide
23
Haematology
Sample Requirements Edition 1
Group and Screen
Blood 4.5 ml Blue Top – see blood
transfusion labelling criteria inside
front cover.
Ham’s Test
See PNH screen.
Haemochromatosis Gene
2 x Blood 3.4 ml Pink Top
Haemoglobin A2
Blood 3.4 ml Pink Top
Haemoglobin Electrophoresis
Blood 3.4 ml Pink Top
Haemoglobin F
Blood 3.4 ml Pink Top
Haemoglobin H Bodies
Blood 3.4 ml Pink Top
Haemoglobin S%
Blood 3.4 ml Pink Top
Haemolysin Screen
Blood 3.4 ml Pink Top +
Blood 7.5 ml Brown Top
Haemosiderin
Urine.
Hb
See Full Blood Count.
HbA2
Blood 3.4 ml Pink Top
HbF
Blood 3.4 ml Pink Top
Hct
See Full Blood Count.
Heparin (Anti FXa) Assay
2 x Blood 3.0 ml Green Top – for LMWH
(eg Clexane®) take 3 – 4 hours post
injection.
Heparin-induced Thrombocytopaenia
Contact Haematology
HFE Gene Status
2 x Blood 3.4 ml Pink Top
Histone Antibodies
Blood 7.5 ml Brown Top
HIT
Contact Haematology
HLA Antibodies
Blood 7.5 ml Brown Top
HLA B27
3 x Blood 3.0 ml Green Top
or 10 ml Green Top
Do not take after 12 noon Friday.
HLA Class I / HLA Class II
Blood 10 ml Green Top
HLA DR2
HLA Typing
3 x Blood 3.0 ml Green Top
or 10 ml Green Top
3 x Blood 10 ml Green Top +
Blood 7.5 ml Brown Top
HNF genes (specify)
Blood 3.4 ml Pink Top
IgA t-Transglutaminase Abs
Blood 7.5 ml Brown Top
IgE
Blood 7.5 ml Brown Top
24
Pathology Laboratory Guide
Haematology Sample Requirements Edition 1
IM Screen
Blood 3.4 ml Pink Top
Immunophenotyping
2 x Blood 3.4 ml Pink Top
Samples must be in laboratory by
12 noon Friday.
Infectious Mononucleosis Screen
Blood 3.4 ml Pink Top
Inhibitor Screen
2 x Blood 3.0 ml Green Top
INR
Blood 3.0 ml Green Top
Intrinsic Factor Antibodies
Blood 7.5 ml Brown Top
Islet Cell Antibodies
Blood 7.5 ml Brown Top
JAK 2
5 x Blood 3.4 ml Pink Top
Jo-1 Antibody
See ENA Antibody Screen.
Kleihauer
Blood 4.5 ml Blue Top
LE Cells
Blood 7.5 ml Brown Top
Live Kidney Donor
4 x Blood 10 ml Green Top
Live Kidney Recipient
Blood 7.5 ml Brown Top
Liver Antibodies
Blood 7.5 ml Brown Top
LKM Antibodies
Blood 7.5 ml Brown Top
Lupus Anticoagulant
2 x Blood 3.0 ml Green Top +
Blood 7.5 ml Brown Top
Lupus Screen
Blood 7.5 ml Brown Top
Lymphocytes
See Full Blood Count.
M2 Antibody
Blood 7.5 ml Brown Top
MAB Antibodies
Blood 7.5 ml Brown Top
Mag Antibody
Blood 7.5 ml Brown Top
Malarial Parasites
Blood 3.4 ml Pink Top – Detail
country visited and prophylaxis.
MCH / MCHC / MCV
See Full Blood Count.
Methotrexate
Blood 7.5 ml Brown Top
Monocytes
See Full Blood Count.
Monospot
Blood 3.4 ml Pink Top
MPO Antibodies
Blood 7.5 ml Brown Top
MTX
Blood 7.5 ml Brown Top
Musk Antibodies
Blood 7.5 ml Brown Top
Pathology Laboratory Guide
25
Haematology
Sample Requirements Edition 1
NBT Test
Contact Haematology.
Neurology DNA tests
3 x Blood 3.4 ml Pink Top
Neuronal Antibodies
Blood 7.5 ml Brown Top
Neutrophil Antibodies
Blood 7.5 ml Brown Top
Neutrophil Function Test
Contact Haematology.
Neutrophils
Blood 3.4 ml Pink Top
NMO Antibodies
Blood 7.5 ml Brown Top
NRBC
Blood 3.4 ml Pink Top
Osmotic Fragility
Contact Haematology.
Ovarian Antibodies
Blood 7.5 ml Brown Top
Paul Bunnell
Blood 3.4 ml Pink Top
PCK2 gene
Blood 3.4 ml Pink Top
P50
Contact Haematology.
PDH Antibodies
Blood 7.5 ml Brown Top
Pemphigoid Antibodies
Blood 7.5 ml Brown Top
Pemphigus Antibodies
Blood 7.5 ml Brown Top
Phospholipid Antibodies
See Lupus Anticoagulant.
Plasma Viscosity (PV)
Blood 3.4 ml Pink Top
Separate EDTA (Pink Top) sample
required for this assay.
Platelet Aggregation
Contact Haematology.
Platelet Antibodies
4 x Blood 3.4 ml Pink Top +
Blood 7.5 ml Brown Top
Platelet Function Tests
Contact Haematology.
Platelets
See Full Blood Count.
PNH Screen
2 x Blood 3.4 ml Pink Top +
Blood 3.4 ml Pink Top from control.
Deliver to Haematology before 12
noon.
PR3 Antibodies
Blood 7.5 ml Brown Top
Protein C
See prothrombotic screen.
Protein S Free
See prothrombotic screen.
Prothrombin Gene Mutation
Blood 3.4 ml Pink Top
Prothrombin Time
Blood 3.0 ml Green Top
26
Pathology Laboratory Guide
Haematology Prothrombotic Screen or Profile
PTS
Sample Requirements Edition 1
2 x Blood 3.0 ml Green Top +
Blood 7.5 ml Brown Top
2 x Blood 3.0 ml Green Top +
Blood 7.5 ml Brown Top
Pyruvate Kinase
Blood 3.4 ml Pink Top
RAST
Blood 7.5 ml Brown Top
RBC
See Full Blood Count.
RDW
See Full Blood Count.
Red Cell Enzymes
Blood 3.4 ml Pink Top
Reticulocytes
Blood 3.4 ml Pink Top
Rheumatoid Factor / RF
Blood 7.5 ml Brown Top
RIPA
Contact Haematology.
Ristocetin Induced Platelet Aggregation
Contact Haematology.
RNP / Sm Antibody
See ENA Antibody Screen.
Ro La Antibodies
See ENA Antibody Screen.
Salivary Duct Antibodies
Blood 7.5 ml Brown Top
Scl-70 Antibody
See ENA Antibody Screen.
Sickle Screen
Blood 3.4 ml Pink Top
Sickle Solubility Test
Blood 3.4 ml Pink Top
Skin ICS Antibody
Blood 7.5 ml Brown Top
Sm Antibody
See ENA Antibody Screen.
Smooth Muscle Antibodies
Blood 7.5 ml Brown Top
Solid Organ GVHD
4 x Blood 10 ml Green Top
Specific IgE
Blood 7.5 ml Brown Top
SSA (Ro) / SSA (La) Antibody
See ENA Antibody Screen
SSB (La) Antibody
See ENA Antibody Screen
Stem Cell Confirmatory Type
2 x Blood 10 ml Green Top
Striated Muscle Antibodies
Blood 7.5 ml Brown Top
T Cell Subsets
2 x Blood 3.4 ml Pink Top
Deliver to Haematology by 1 pm.
Thalassaemia Screen
Blood 3.4 ml Pink Top
Thick Film for Malaria Parasites
Blood 3.4 ml Pink Top
Thrombin Time
Blood 3.0 ml Green Top
Pathology Laboratory Guide
27
Haematology
Sample Requirements Edition 1
Thrombophilia Screen
2 x Blood 3.0 ml Green Top +
Blood 7.5 ml Brown Top
Tissue Transglutaminase Antibodies (TTG)
Blood 7.5 ml Brown Top
Tissue Typing
Blood 7.5 ml Brown Top
Total IgE
Blood 7.5 ml Brown Top
Trial SHNL
Blood 7.5 ml Brown Top
TTG
Blood 7.5 ml Brown Top
U1-snRNP Antibody
See ENA Antibody Screen
Vasculitis Screen
Blood 7.5 ml Brown Top
Voltage Gated Ca++ Channel Antibodies
Blood 7.5 ml Brown Top
Voltage Gated K+ Channel Antibodies
Blood 7.5 ml Brown Top
Von Willebrand Disease (VWD) Screen
See Factor VIII Profile
vWF Activity / vWF Antigen
See Factor VIII Profile
vWF Multimers
3 x Blood 3.0 ml Green Top
WBC
See Full Blood Count.
ZAP 70
3 x Blood 3.4 ml Pink Top
28
Pathology Laboratory Guide
Microbiology Sample Requirements Edition 1
Microbiology Department – Tel: 5736 (01473 703736)
TEST
SAMPLE
Adenovirus CFT
Blood 7.5 ml Brown Top
Adenovirus DNA
Blood 3.4 ml Pink Top
Alpha virus
Blood 7.5 ml Brown Top
Amikacin Assay
Blood 7.5 ml Brown Top
Amoebic FAT
Blood 7.5 ml Brown Top
Anti Fungal Assays
Blood 7.5 ml Brown Top
Anti Staph Ab
Blood 7.5 ml Brown Top
ASO Titre
Blood 7.5 ml Brown Top
Aspergillus pptn
Blood 7.5 ml Brown Top
Avian pptn
Blood 7.5 ml Brown Top
Bartonella Ab
Blood 7.5 ml Brown Top
Bordetella pertussis Ab
Blood 7.5 ml Brown Top
Borrelia (Lyme)
Blood 7.5 ml Brown Top
Brucella Ab
Blood 7.5 ml Brown Top
Campylobacter Ab
Blood 7.5 ml Brown Top
Candida Ag
Blood 7.5 ml Brown Top
Cat Scratch Ab (Bartonella)
Blood 7.5 ml Brown Top
Chlamydia pneumoniae Ab
Blood 7.5 ml Brown Top
CMV IgG / IgM
Blood 7.5 ml Brown Top
CMV PCR
Blood 3.4 ml Pink Top
Colistin assay
Blood 7.5 ml Brown Top
Corynebacterium diphtheriae Immunity
Blood 7.5 ml Brown Top
Co-trimoxazole Assay
Blood 7.5 ml Brown Top
Coxiella burnettii
Blood 7.5 ml Brown Top
Cryptococcus Ag
Blood 7.5 ml Brown Top
Dengue Ab
Blood 7.5 ml Brown Top
EBV DNA Viral Load
Blood 3.4 ml Pink Top
EBV Serology
Blood 7.5 ml Brown Top
Pathology Laboratory Guide
29
Microbiology
Sample Requirements Edition 1
Efavirenz Assay
Blood 7.5 ml Brown Top
Enterovirus PCR
Blood 7.5 ml Brown Top
Enterovirus CFT
Blood 7.5 ml Brown Top
Escherichia coli 0157 Serology
Blood 7.5 ml Brown Top
Farmers Lung pptns
Blood 7.5 ml Brown Top
Filaria Ab
Blood 7.5 ml Brown Top
Flavivirus Ab
Blood 7.5 ml Brown Top
Flucytosine
Blood 7.5 ml Brown Top
H. influenzae b immunity
Blood 7.5 ml Brown Top
Haemorrhagic Fever Viruses –
Serol / PCR / Culture
Contact Microbiology before
sample collection.
Helicobacter pylori Ab
Blood 7.5 ml Brown Top
Hep A IgM / HAV Tot
Blood 7.5 ml Brown Top
Hep B Ag Confirm
Blood 7.5 ml Brown Top
Hep B core Ab
Blood 7.5 ml Brown Top
Hep B Surface Ab (anti-HBs)
Blood 7.5 ml Brown Top
Hep B Virus DNA
Blood 7.5 ml Brown Top
Hep C Ab
Blood 7.5 ml Brown Top
Hep C PCR
Blood 7.5 ml Brown Top
Hep C Viral Load / Genome
Blood 7.5 ml Brown Top
Send to laboratory immediately.
Hep D Virus Ab / Ag
Blood 7.5 ml Brown Top
Hep E Ab / Ag
Blood 7.5 ml Brown Top
Histoplasma Ab
Blood 7.5 ml Brown Top
HIV Ab / Ag
Blood 7.5 ml Brown Top
HIV Proviral DNA
Blood 3.4 ml Pink Top
HIV Viral Load / RNA
2 x Blood 3.4 ml Pink Top
H. Pylori ab
Please contact Microbiology.
HSV IgG
Blood 7.5 ml Brown Top
HTLV 1 & 2 Genome
Blood 3.4 ml Pink Top
HTLV 1 & 2 Serology
Blood 7.5 ml Brown Top
Human papilloma virus (HPV) PCR
Cervical swab-smear / biopsy.
30
Pathology Laboratory Guide
Microbiology Sample Requirements Edition 1
Hydatid Ab
Blood 7.5 ml Brown Top
Immunoglobulins for pairing with CSF
Blood 7.5 ml Brown Top
Influenza A / B CFT
Blood 7.5 ml Brown Top
Itraconazole Levels
Blood 7.5 ml Brown Top
Legionella Ab
Blood 7.5 ml Brown Top
Legionella Ag (within 7 days of onset only)
Urine (plain bottle).
Leishmania Ab
Blood 7.5 ml Brown Top
Leptospirosis Ab
Blood 7.5 ml Brown Top
Lopinavir (Kaletra) Assay
Blood 9.0ml Orange Top
Lyme Ab
Blood 7.5 ml Brown Top
Measles IgG
Blood 7.5 ml Brown Top
Measles PCR
Blood 3.4 ml Pink Top
Mumps Ab
Blood 7.5 ml Brown Top
Mycoplasma IgM
Blood 7.5 ml Brown Top
N. meningitidis Ab (convalescent)
Blood 7.5 ml Brown Top
N. meningitidis DNA (acute)
Blood 3.4 ml Pink Top
Nevirapine Level
Blood 3.4 ml Pink Top
Oligoclonal Bands
Blood 7.5 ml Brown Top
Parainfluenza CFT
Blood 7.5 ml Brown Top
Parvovirus (Erythrovirus) IgG / IgM
Blood 7.5 ml Brown Top
Polyoma BK virus DNA
Blood 3.4 ml Pink Top
Polyoma JC virus DNA
Blood 3.4 ml Pink Top
Pneumococcal Ab
Blood 7.5 ml Brown Top
Procalcitonin Assay (by special arrangement)
Blood 7.5 ml Brown Top
Psittacosis Ab
Blood 7.5 ml Brown Top
Q Fever
Blood 7.5 ml Brown Top
Rabies Serology
Blood 7.5 ml Brown Top
Resistant Screening and Viral Load
Blood 2 x Blood 4.5 ml Blue Top
Respiratory Serology
Blood 7.5 ml Brown Top
Rickettsia serology
Blood 7.5 ml Brown Top
RSV CFT
Blood 7.5 ml Brown Top
Pathology Laboratory Guide
31
Microbiology
Sample Requirements Edition 1
Rubella IgG
Blood 7.5 ml Brown Top
Rubella IgM
Blood 7.5 ml Brown Top
Schistosomal Ab
Blood 7.5 ml Brown Top
Spotted Fever (group)
Blood 7.5 ml Brown Top
Streptomycin Assay
Blood 7.5 ml Brown Top
Strongyloides Ab
Blood 7.5 ml Brown Top
Syphilis (TPA)
Blood 7.5 ml Brown Top
TB T-spot
2 x Blood 7.5 ml Orange Top.
Contact Microbiology before
collection.
Teicoplanin Assay
Blood 7.5 ml Brown Top
Tetanus Immunity
Blood 7.5 ml Brown Top
Tobramycin Assay
Blood 7.5 ml Brown Top
Toxocara Ab
Blood 7.5 ml Brown Top
Toxoplasma Ab
Blood 7.5 ml Brown Top
Transplant screen (Renal Unit)
Blood 7.5 ml Brown Top
Treponema Pallidum Antibodies (TPA)
Blood 7.5 ml Brown Top
Trichinella Ab
Blood 7.5 ml Brown Top
Trimethoprim Assay
Blood 7.5 ml Brown Top
Tropheryma whipplei (PCR)
CSF sample. Contact laboratory.
Trypanosomal
Blood 7.5 ml Brown Top
Varicella Zoster Virus (VZW) IgG & IgM
Blood 7.5 ml Brown Top
VDRL
Blood 7.5 ml Brown Top
VZV IgG
Blood 7.5 ml Brown Top
VZV IgM
Blood 7.5 ml Brown Top
Yersinia Serology
Blood 7.5 ml Brown Top
32
Pathology Laboratory Guide
Microbiology Sample Requirements Edition 1
Urine Specimens
Urine containers are provided. These contain boric acid as a preservative. Urine
containers should be filled to the mark. The specimen will remain useful for up
to 48 hours at room temperature.
Small volumes of urine should be collected into plain universals and should
arrive at the laboratory within 4 hours or within 24 hours if refrigerated.
Collection of Mid-stream Urine
Instructions to the patients should be as follows:
1 Carefully wash the external genitalia and dry with a clean towel.
2Unscrew the top of the sterile container, avoiding touching the inside of
the bottle or lid.
3 Start passing urine, allowing the first part to flow into the pan.
4Collect the next part of the specimen straight into the container (women
should separate the labia with the fingers of the hand which is not holding
the sterile container). The bottle should be filled.
5Screw the lid on the container firmly. Wipe and dry the outside of the
container and write your name and the date on the label.
Collection of Urine from Infants
1 Bag Urine
External genitalia should be washed and dried before collection. Specimen
should be decanted into a sterile plain universal.
2 Supra Pubic Aspirate
These should be decanted into a sterile plain universal – these are treated
as sterile fluids and should be sent to the laboratory immediately.
Indwelling Catheter Samples
Urine should be aspirated with needle and syringe from the catheter sampling
port after disinfection of the port with alcohol.
Urine for Suspected Tuberculosis
At least 3 full early morning urine samples should be collected into large
containers (do not use small boric acid pots).
Pathology Laboratory Guide
33
Sample Requirements Edition 1
General Information
Sample Requirements Booklet 2012
I trust you find the booklet of sample requirements for the pathology
laboratories helpful. We intend to update it each year – please destroy any
copies from previous years.
Further copies can be obtained from Pathology reception or by completing
and returning the form at the end of this booklet.
Thank you to everyone who has contributed suggestions for improvements
to this booklet. Please continue to send suggestions by completing the form
opposite.
Please return the form to:
Stephen Gee
Clinical Biochemistry Department
Post Bag C363
Ipswich Hospital
Heath Road
Ipswich
IP4 5PD
Tel: 01473 703708
Email: [email protected]
34
Pathology Laboratory Guide
General Information Sample Requirements Edition 1
Feedback on Sample Requirements Booklet 2012
I make the following suggestions for improving the booklet:
Comments:
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Pathology Laboratory Guide
additional copies sent to:
Kindly sponsored by Sarstedt Ltd.
Produced by:
Pathology Laboratory
The Ipswich Hospital NHS Trust
Heath Road, Ipswich, Suffolk IP4 5PD
Direct tel: 01473 703721
Hospital switchboard: 01473 712233
www.ipswichhospital.nhs.uk
January 2012
© The Ipswich Hospital NHS Trust, 2006-2012. All rights reserved.
Not to be reproduced in whole, or in part, without the permission of the copyright owner.
DPS ref: 13197-11.indd