Sample preparation
Primer Sample preparation RNA extraction Sequence WNV-multi-forward 5’-GCACGAAGATCTCGATGTCTAAG-3’ WNV-multi-reverse 5’-ATTCCGCGTTTTAGCATATTGAC-3’ JE-multi-forward 5’-AGAACGGAAGAYAACCATGACTAAA-3’ JE-multi-reverse 5’-CCGCGTTTCAGCATATTGAT-3’ Multi-probe FAM-5’-ACCAGGAGGGCCCGG-3’MGB WNV-multi-forward 5’-GCACGAAGATCTCGATGTCTAAG-3’ JE-multi-forward 5’-AGAACGGAAGAYAACCATGACTAAA-3’ WNV-multi-reverse 5’- ATTCCGCGTTTTAGCATATTGAC-3’ JE-multi-reverse 5’-CCGCGTTTCAGCATATTGAT-3’ Multi-probe FAM-5’-ACCAGGAGGGCCCGG-3’MGB Multi-probe FAM-5’-ACCAGGAGGGCCCGG-3’MGB
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