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Automated Workflows from Sample to Result
Sample & Assay Technologies
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QIAGEN provides cutting-edge automated solutions that cover each step of the life science or
molecular diagnostics research laboratory workflow — from sample to result. Regardless of whether
you work in academic, clinical, or commercial research, in vitro diagnostics, or standardized
testing, automated sample and assay technologies standardize your workflow and deliver highquality, reliable data. We provide innovative automated solutions to suit all your application
requirements and daily throughput needs — from sample disruption to analysis and detection.
Purification of nucleic acids or proteins followed by analysis is labor-intensive and involves numerous
tedious and time-consuming steps. Our award-winning automated solutions with fully integrated, proven
QIAGEN® kits enable rapid, standardized purification of target molecules from a wide
variety of starting materials. Dedicated solutions with optimized chemistries for assay setup and
detection and analysis deliver the high-quality results you need in your downstream applications. Our
automated solutions can be seamlessly integrated into your complete workflow, eliminating manual
handling errors and enabling you to free up your time. Furthermore, QIAGEN automated solutions are
easy to use and can be operated by all lab personnel — from the automation novice to the expert.
In addition to Swiss precision and quality, we provide comprehensive support services to assure
fast startup and continued success of your automated applications. QIAGEN Field Service provides
a wide range of flexible support agreements tailored to your needs.
Automated workflows from sample to result
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www.qiagen.com
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Automation Brochure 01/2009
Automated Solutions for Sample Disruption
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TissueRuptor® and TissueLyser II systems
The TissueRuptor — a handheld rotor–stator homogenizer that uses disposable probes — provides
rapid and efficient low-throughput disruption of animal and human cells or tissues and plant material.
The TissueLyser II provides fast, medium- to high-throughput disruption of up to 2 x 96 samples of
tissue, plant material, yeast, or bacteria in less than 5 minutes.
I Rapid and efficient disruption of a wide range of sample types
I Minimized risk of cross-contamination
I Reproducible, standardized disruption
I Seamless integration with QIAGEN sample purification technologies
Key features
TissueRuptor
TissueLyser II
Sample throughput
Single sample
Up to 48 or 192 samples in parallel. Adapter Sets for
flexible sample throughput (2 x 24, 2 x 96)
Disruption technology
Rotor–stator; disposable probes
Bead mill; steel, tungsten carbide, or glass beads
Disruption time
>30 seconds
<5 minutes
A
Skin
RNAlater
Frozen
Liver
RNAlater
Frozen
Muscle
RNAlater
TissueLyser II
B
Frozen
Efficient disruption and homogenization of animal tissues. Tissues (20 mg) were either frozen (Frozen) or stabilized in RNAlater®
RNA Stabilization Reagent (RNAlater), and then disrupted using the TissueLyser II (2 x 2 minutes for liver and muscle; 2 x 5
minutes for skin). RNA was purified using the RNeasy® Mini Kit (liver), RNeasy Fibrous Tissue Mini Kit (skin), or RNeasy Lipid
Tissue Mini Kit (muscle). A Analysis on a 1.2 % formaldehyde agarose gel shows sharp ribosomal RNA bands, indicating
intact RNA. B RNA yields were quantified by A260 nm absorbance measurements.
M
M
Reproducible DNA purification from animal tissue. The
heart was excised from a single rat and cut into 96 x 25
mg pieces, which were then disrupted using the TissueLyser
II (2 x 15 seconds). Automated DNA purification was
carried out on the QIAcube using the DNeasy® Blood &
Tissue Kit (with Reagent DX to minimize lysate foaming).
Analysis of 48 samples on an agarose gel showed
consistent amounts of DNA from each sample.
M: GelPilot® Lambda HindIII marker.
Automation Brochure 01/2009
Sample & Assay Technologies
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Automated Solutions for Purification
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QIAcube®
The award-winning QIAcube simplifies sample prep by enabling walkaway automation of trusted
QIAGEN spin-column kits. All steps in the purification procedure are automated, and up to
12 samples can be processed per run. The continuously expanding range of protocols has now
grown to almost 80 standard protocols. For an up-to-date list of protocols, visit the QIAcube Web
Portal at www.qiagen.com/MyQIAcube.
I Purification of DNA, RNA, or proteins
I Automation of trusted QIAGEN spin-column kits
QIAcube
I Elimination of manual processing steps
I More free time with affordable automated processing
I Standardized results and increased productivity
New
QIAxtractor
The QIAxtractor is a compact instrument that provides walkaway purification of nucleic acids from
8–96 samples per run. The easy-to-use, economical system comprises instrument, reagents,
plasticware, and optimized protocols, enabling purification of nucleic acids from a wide range of
sample types. The instrument has low tip consumption, reducing waste and improving cost-efficiency.
I Fully automated purification in 96-well format
I Fast processing of 96 samples in 96 minutes
I Compact benchtop instrument
QIAxtractor
I Economical sample preparation
I Pre- and post-run documentation
BioRobot® Universal System
The BioRobot Universal System integrates all the instrumentation, software, purification technologies,
and enzyme technologies that are required for medium- to high-throughput applications in 96-well
format. Standardized, highly reproducible nucleic acid purification and reaction setup at the front-end
of your molecular biology applications ensure consistent, reliable results that can be compared
across experiments and projects.
I Reproducible, standardized results between experiments and labs
BioRobot Universal System
I Fast startup and immediate results with optimized, ready-to-run protocols
I Comprehensive service agreements tailored to your needs
I Widest range of applications in 96-well format
I Nucleic acid purification and subsequent assay setup
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Automation Brochure 01/2009
Automated Solutions for Purification
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EZ1® Advanced instruments
The EZ1 Advanced* and EZ1 Advanced XL* enable rapid purification of nucleic acids from 1–6
samples and 1–14 samples, respectively, using proven magnetic-particle technology. New features
enable effortless data management and provide secure user environments. The combination of
easy-to-use instrument with error-free protocol selection and worktable setup makes nucleic acid
purification easy.
I High-quality genomic DNA and RNA from a broad range of sample materials
I Viral nucleic acids from serum, plasma, and CSF
I Effortless data management with full traceability
I UV decontamination for a safe user environment
I Flexible throughput by connecting multiple units
EZ1 Advanced and
EZ1 Advanced XL
QIAsymphony® SP
The QIAsymphony SP,† the first module in the QIAsymphony product line, meets the demand for
flexible sample preparation of nucleic acids and 6xHis-tagged proteins from a wide range of starting
materials. Up to 96 samples — in batches of up to 24 samples — can be processed per run, with
sample volumes up to 1 ml. The broad range of applications combined with the exceptional
purification efficiency and process safety of the QIAsymphony SP place it at the cutting edge of
laboratory automation for all disciplines in molecular biology, including in vitro diagnostics.† The
QIAsymphony SP can be seamlessly integrated with the QIAsymphony AS assay setup module
(page 7), and is compatible with real-time PCR applications on the new Rotor-Gene Q (page 8),
enabling automation of your entire workflow — from sample to result.
I Easy-to-use with built-in touchscreen and novel, prefilled reagent cartridges
QIAsymphony SP
I Purification of DNA, RNA, viral and bacterial nucleic acids, 6xHis-tagged proteins
I In-process sample loading and queuing enables flexible system use
I Remote Service (QDM) and data management via network or USB
I Workflow extension with the QIAsymphony AS module
* Available for in vitro diagnostics applications. Not available in all countries; please inquire.
†
Instrument will be available for in vitro diagnostics applications; please inquire.
Automation Brochure 01/2009
Sample & Assay Technologies
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Automated Solutions for Purification
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BioRobot MDx
The BioRobot MDx* provides the same high-quality purification as manual QIAGEN procedures, but
with automated high-throughput processing of up to 96 samples per run — and up to 300 preps
per day. Highly pure nucleic acids are ready in 2–3 hours (depending on the sample type and
nucleic acids purified). The BioRobot MDx provides fully automated, high-throughput purification
of DNA and RNA from a range of human sample types using trusted QIAamp® and PAXgene® Kits.
BioRobot MDx
It is particularly well-suited for high-throughput human molecular diagnostics applications such as
studies of infectious disease, genetic testing, genotyping, and oncology research.
I Secure sample and data management
I Easy to use and highly reliable error-free processing
I Flexible throughput and applications
I Optimized and customized protocols using proven chemistries
I Process safety through full bar code and sample tracking
Autopure LS®
The Autopure LS enables automated purification of genomic DNA from sample volumes of
1–10 ml using proven Puregene® chemistry. Purified DNA is highly stable and well-suited for
archiving. For increased convenience and flexibility, samples can be processed in batches of 8 or 16.
I Yields of up to 350 µg DNA per 10 ml fresh whole blood
I DNA from a broad range of sample types and sizes
I Proven Puregene chemistries with 16 years of DNA stability data
I Purification of highly stable DNA ready for use or archiving
I Streamlined, efficient workflow
Autopure LS
* Available for in vitro diagnostics applications. Not available in all countries; please inquire.
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Automation Brochure 01/2009
Automated Solutions for Assay Setup
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New
QIAsymphony AS
The QIAsymphony AS* extends the capabilities of the QIAsymphony SP (page 5) to include automated
assay setup. The new module interfaces with the QIAsymphony SP and is operated via the same
easy-to-use, intuitive software. For integrated operation, samples processed on the QIAsymphony
SP can be transferred to the QIAsymphony AS, reducing manual handling steps and documentation.
The QIAsymphony AS provides active cooling of reagents, eluates, and assays, enabling safe and
reproducible assay setup. The QIAsymphony AS enables setup of multiple assays per run or
sample and supports artus® products and other QIAGEN products for PCR.* Extend your
automation workflow with the new QIAsymphony AS and reduce hands-on time for nucleic acid
purification and assay setup. The QIAsymphony series of instruments can also be combined with
QIAsymphony AS
the new Rotor-Gene Q (page 8), enabling automation of complete workflows — from sample to result.
I Automated, integrated assay setup module complements the QIAsymphony SP
I Convenient, easy-to-use sample-oriented software
I Reduced hands-on time and convenient electronic documentation
I Customization service for in-house assays
I Seamless integration with QIAGEN sample and assay technologies
New
QIAgility™
The new QIAgility is a compact benchtop instrument that enables automated PCR setup in a wide
range of formats, including PCR assays for the Rotor-Gene Q (page 8). The high precision of the
QIAgility delivers the reproducible results you need in your quantitative PCR assays, from sample
to sample and experiment to experiment. Automated PCR setup is rapid and reliable and eliminates
manual pipetting steps that can be prone to human error. The unmatched versatility of the QIAgility
means that almost all tube and plate formats are supported, as well as rotors for the Rotor-Gene
Q. For increased ease of use, the software reflects the setup of the instrument worktable. Optimized
protocols assure fast startup and immediate results. The flexibility of the software enables the user
QIAgility
to develop software protocols to suit specific application requirements, allowing the QIAgility to
easily be adapted to changing research needs.
I Automated PCR setup in all formats
I Convenient, easy-to-use software
I Standardized results and increased productivity
I Elimination of manual pipetting steps
I Seamless integration with QIAGEN sample technologies
* For information about product and protocol availability, please inquire. Instrument will be available for in vitro diagnostics
applications; please inquire.
Automation Brochure 01/2009
Sample & Assay Technologies
7
Automated Solutions for Detection and Analysis
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New
Rotor-Gene™ Q
The unique centrifugal rotary design of the Rotor-Gene Q* makes it the world’s most precise and
versatile real-time PCR instrument. Each tube spins in a chamber of moving air, which keeps all
samples at each step of the cycling program at exactly the same temperature. Optical
detection is equally precise since each sample rotates past the excitation and detection optics. This
unique design delivers sensitive and fast PCR analysis and eliminates the variation that typically
occurs in block-based cyclers. With up to 6 channels spanning UV to infrared wavelengths, the
Rotor-Gene Q
Rotor-Gene Q delivers the greatest optical range of any real-time cycler. Interchangeable rotors
enable analysis in different sample volumes and tube formats, which are all fully supported by the
QIAgility (page 7) for automated PCR setup.
With the Rotor-Gene Q, you have the choice of a broad range of proven QIAGEN kits and assays
for gene expression analysis, pathogen detection, veterinary diagnostics, genotyping, epigenetics,
and miRNA research. Advanced instrument design enables performance of demanding
applications such as high-resolution melting (HRM) analysis, allowing, for example, reliable
detection of single base changes. The comprehensive software enables state-of-the art real-time
PCR and HRM analysis.
I Outstanding thermal and optical performance
I More applications than any other real-time cycler
I Unmatched optical range with up to 6 channels
I Robust design enables easy setup and minimal maintenance
I Compatible with the QIAgility for automated PCR setup
Rotor-Gene Q cycler. Heating/cooling is achieved by rapid airflow in the reaction chamber.
Tubes spin past the excitation/detection optics every 150 milliseconds. High-speed data
capture is provided by a highly sensitive photomultiplier detector.
QIAxcel™ System
The QIAxcel is a multicapillary electrophoresis system designed to overcome the bottlenecks of gel
electrophoresis. The fully automated system can process up to 96 samples per run. The optimized
system, including gel cartridge and BioCalculator software, delivers reliable results in a wide range
of applications, and provides unrivaled resolution, speed, and throughput in DNA and RNA analyses.
I Automated analysis of DNA fragments and RNA
I Analysis of 12 DNA samples in less than 10 minutes
QIAxcel
I Safety and convenience with ready-to-use gel cartridges
I Accurate analyses with resolution down to 3–5 bp
I Standardized processing with digital data collection and documentation
* Available for in vitro diagnostics applications. Not available in all countries; please inquire.
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Automation Brochure 01/2009
Automated Solutions for Detection and Analysis
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PyroMark Q24
The PyroMark Q24* uses proven Pyrosequencing® technology for real-time, sequence-based
detection and quantification for genetic testing and epigenetic methylation analysis. The unique
PyroMark system combines reliable quantification and sequencing results in minutes. The PyroMark
Q24 is therefore highly suited for the discovery and analysis of rare mutations and methylation
sites. The PyroMark Q24 system provides a sequencing instrument, sample preparation
workstation, and reagents and controls, as well as comprehensive and flexible analysis software.
The versatile PyroMark Q24 seamlessly integrates into epigenetics and genetic analysis workflows,
and complements QIAGEN’s advanced technologies for sample preparation, bisulfite conversion,
and PCR amplification.
I Highly accurate results in epigenetics and cancer research
PyroMark Q24
I Reliable quantification of allele representation and methylation status
I Sequence information enables discovery of rare mutations and CpG sites
I Methylation analysis can be combined with SNP typing in one assay
I 1–24 samples can be analyzed in parallel in as little as 15 minutes
LiquiChip® 200 Workstation
The LiquiChip 200 Workstation is used to analyze bead-based interaction assays, including
detection of pathogens using QIAplex® Panels and also protein-based interaction assays. A system
of bead color-coding enables up to 100 separate interactions to be analyzed simultaneously
within a single sample. QIAplex Panels are specifically developed for the LiquiChip Workstation
and allow multiplex detection of molecular targets including nucleic acids specific for respiratory
pathogens. Ease of use is provided by the QIAplex MDD Software, which provides a convenient
user interface.
I Multiplexing provides more information using less sample
LiquiChip 200 Workstation
I Flexible system allows easy and rapid assay development
I Most up-to-date xMAP® instrumentation
* Available for in vitro diagnostics applications. Not available in all countries; please inquire.
Automation Brochure 01/2009
Sample & Assay Technologies
9
QIAGEN Field Service — Your Partner in Continued Success
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Peace of mind through unrivaled instrument and application support
QIAGEN Field Service provides comprehensive support services for laboratory instruments, in life
science research and in vitro diagnostics laboratories, to ensure the continued success of your
automated applications.
I Worldwide service
I Expertise in automation and application support
I Quick response times
QIAGEN Field Service offers a wide range of flexible Service Support products, giving you peace
of mind and letting you enjoy complete coverage and cost control. We work in partnership with
you to establish proven QIAGEN applications on your system. Our Application Services and
Training Programs give you the freedom and flexibility to adapt your system to specific or changing
research needs. With ISO 9001/ISO 13485 certification and an international team of highly
qualified and experienced Support Specialists, we deliver the high-quality service that you deserve
and that your applications demand.
I Flexible Service Support Agreements for coverage tailored to your needs
I Repairs and upgrades with all parts certified and guaranteed
I Application Services for creating and optimizing applications
I Preventive Maintenance to assure optimal performance and maximum productivity
I Qualification Services with full documentation and performance of IQ/OQ*
I Warranty Extensions to give you complete coverage and cost control
* Not available for all systems; please contact your local Instrument Service Department for more information.
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Automation Brochure 01/2009
The QIAcube, EZ1 Advanced, EZ1 Advanced XL, QIAsymphony SP, QIAxcel System, QIAxcel Fast Analysis System, and
PyroMark Q24 are intended to be used only in combination with QIAGEN kits indicated for use with the QIAcube, EZ1
Advanced, EZ1 Advanced XL, QIAsymphony SP, QIAxcel System, QIAxcel Fast Analysis System, or PyroMark Q24 for the
applications described in the respective kit handbooks.
The QIAxtractor is intended for molecular biology applications. This product is neither intended for the diagnosis,
prevention, or treatment of a disease, nor has it been validated for such use either alone or in combination with other
products. Therefore, the performance characteristics of the product for clinical use (i.e., diagnostic, prognostic, therapeutic,
or blood banking) is unknown.
The BioRobot MDx is intended for laboratory use. No claim or representation is intended for its use to provide information
for the diagnosis, prevention, or treatment of a disease.
The BioRobot MDx DSP is intended for in-vitro diagnostic use in Europe.
The Autopure LS instrument is intended to be used only in combination with Autopure Reagents for applications described
in the Autopure LS User Manual.
The QIAgility, TissueRuptor, TissueLyser II, BioRobot Universal System, and LiquiChip 200 Workstation are intended for
molecular biology applications. These products are neither intended for the diagnosis, prevention, or treatment of a disease,
nor have they been validated for such use either alone or in combination with other products.
The Rotor-Gene Q, if used in combination with QIAGEN kits indicated for use with the Rotor-Gene Q instrument, is
intended for the applications described in the respective QIAGEN kit handbooks. If used with kits other than QIAGEN kits,
it is the user’s responsibility to validate the performance of such product combination for any particular application.
The QIAsymphony AS will be available soon.
“RNAlater®” is a trademark of AMBION, Inc., Austin, Texas and is covered by various U.S. and foreign patents.
Visit www.qiagen.com/goto/automation and find out how to automate your workflow —
from sample to result!
Trademarks: QIAGEN®, QIAamp®, QIAcube®, QIAgility™, QIAplex®, QIAsymphony®, QIAxcel™, artus®, Autopure LS®, BioRobot®, EZ1®, DNeasy®, GelPilot®, LiquiChip®, Puregene®, Pyrosequencing®, QuantiTect®, RNeasy®,
TissueRuptor® (QIAGEN Group); PAXgene® (PreAnalytiX GmbH); Rotor-Gene® (Corbett Research); SYBR® (Molecular Probes, Inc.); xMAP® (Luminex Corporation).
The QIAGEN silver logo is exclusively licensed to Corbett Research.
NOTICE TO PURCHASER:
The purchase of this product (Rotor-Gene Q, Rotor-Disc) includes a limited, non-transferable license to certain patents (see details below) surrounding rapid polymerase chain reaction (PCR) methods and instrumentation, the
use of SYBR® Green I in PCR reactions, melting curve analysis, analysis methods of DNA melting data, specifically high resolution melting (HRM) and others.
The purchase of this product (Rotor-Gene Q, Rotor-Disc) includes a limited, non-transferable license to one or more of US Patents Nos 6,787,338; 7,238,321; 7,081,226; 6,174,670; 6,245,514; 6,569,627; 6,303,305;
6,503,720; 5,871,908; 6,691,041; 7,387,887; and U.S. Patent Applications Nos. 2003-0224434 and 2006-0019253 and all continuations and divisionals, and corresponding claims in patents and patent
applications outside the United States, owned by the University of Utah Research Foundation, Idaho Technology, Inc., and/or Roche Diagnostics GmbH, for internal research use or for non-in vitro diagnostics applications.
No right is conveyed, expressly, by implication or estoppel, for any reagent or kit, or under any other patent or patent claims owned by the University of Utah Research Foundation, Idaho Technology, Inc., and/or Roche
Diagnostics GmbH, or by any other Party. For information on purchasing licences for in-vitro diagnostics applications or reagents, contact Roche Molecular Systems, 4300 Hacienda Drive, Pleasanton, CA 94588, USA.
ADDITIONAL NOTICE TO PURCHASER IN USA, EUROPE, AND AUSTRALIA ONLY:
Practice of the polymerase chain reaction (PCR) process may require a license depending on the reagents and methods applied. Purchase of this instrument (Rotor-Gene Q) does not itself convey to the purchaser a complete
license or right to perform the PCR process. The use of this instrument (Rotor- Gene Q) with Authorized Reagents such as Authorized QIAGEN kits and assays provides a limited PCR license in accordance with the label
rights accompanying such reagents.
Further information on purchasing licenses to practice the PCR process may be obtained by contacting the Director of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA and/or
Roche Molecular Systems, 4300 Hacienda Drive, Pleasanton, CA 94588, USA. No right is conveyed expressly, by implication or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or
methods such as 5' nuclease methods.
1054489 01/2009
© 2009 QIAGEN, all rights reserved.
Automation Brochure 01/2009
Sample & Assay Technologies
11
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