Systems and Solutions for EM Sample Preparation The New Product Portfolio from Leica Microsystems Living up to Life EM Sample Preparation – Biological Samples Ultramicrotomy Leica EM UC6 Leica EM FC6 Leica EM KMR2 Contrasting/Labelling Leica EM IGL Leica EM AC20 Processing Leica EM AFS2/EM FSP Leica EM TP Leica EM AMW Trimming Leica EM TRIM2 Leica EM RAPID Cryo Fixation Leica EM PACT2/EM RTS HPM100 JFD030 Leica EM CPC Leica EM MM80 E Freeze Etching/Freeze Fracture BAF060 MED020 FF Cryo Transfer VCT100 Coating MED020 SCD500 SCE005/SCD050 CED030 QSG100 Drying MED020 FD CPD030 2 The New Product Portfolio from Leica Microsystems ❙❘ Ultramicrotomy ❙❘ Contrasting/Labelling ing Leica EM UC6 Ultramicrotome for ultrathin sectioning of biological and industrial specimen samples. • Optimal positioning of the stereo microscope • Optimized antistatic device • Sectioning without external and internal vibrations • Fully motorized knife stage • Brightness-controlled multi-LED illumination Leica EM IGL Automated immunogold labelling system. stem. • Simultaneous labelling of 24 gridss • Time saving • Minimized cross contamination Leica EM FC6 Low temperature sectioning system for ultrathin cryosectioning of biological and industrial specimen n samples. • Ergonomic design for fatigue-freee operation • Internal chamber illumination • Temperature range from -185° to -15°C • Individual temperature for specimen, knife and gas Leica EM KMR2 Glass knifemaker for producing 45° glass knives from 6.4, 8 and 10 mm glass utilizing the balanced-break method. • Easy to use • Reproducible Leica EM AC20 Automatic contrasting of ultrathin sections for Electron Microscopy. • Safe • Lower reagent consumption • High contrast • Reproducible results Leica EM AMW Automatic microwave tissue processor for Electron Microscopy. • Fast processing • Minimum user interface ay • From fresh tissue to TEM the same day ❙❘ Trimming ❙❘ Processing Leica EM AFS2 Freeze substitution and low temperature embedding for light and electron microscopy. opy. • -140°C to +70°C working range • Transfer function • Fast UV polymerization • Intuitive programming Leica EM FSP An automatic reagent handling/dispensing system for freeze substitution and PLT. • One step preparation • Safe and convenient • Flexible built-in UV light • Up to 20 samples per run 3 Leica EM TP Automated routine tissue processor.. • Safe • Time saving • Reproducible results Leica EM TRIM2 Specimen trimming device for TEM, SEM, M, LM. 1 µ step advance • • Perpendicular viewing of the sample • LED illumination Leica EM RAPID Advanced specimen trimming device for TEM, SEM, LM. • 0.5,1,10,100 µm step advance • Adjustable cutting speed 300–20000 pm • Advanced indication on LCD displayy • Unique system for preparation of tablets for pharma industry ❙❘ Cryo Fixation Leica EM CPC Universal cryoﬁxation system for immersion ion and metal mirror cryoﬁxation. • Versatile • Easy change of modules • Schematic display • Also for use as temperature controlled cryopreparation chamber Leica EM PACT2 with EM RTS High pressure freezer with rapid transfer system. • Freezing of specimens, up to 200 μm in thickness and 1.5 mm in diameter without ice crystal ystal damage ng jet of LN2 • High cooling rates by strong • Rapid turnaround time • Compact and mobile • Automatic bake-out cycle • Internal memory with download on USB • Dewar with drain • Low LN2 consumption • Low noise M/EM • Suitable for correlative LM/EM Leica EM MM80 E Metal mirror croﬁxation system for impact freezing. ng. • Gold-coated copper blocks • Short turnaround time • Pneumatically damped sample holder HPM100 High pressure freezing system for freezing of specimens up to 200 µm in thickness and 6 mm in diameter without hout ice crystal damage. • No cryo protectant required • Cryo-ﬁxation of suspensions, monolayer cell cultures and tissues es ng • Short handling time before freezing • Reproducible freezing • Easy one button operation JFD030 Jet freezing device for ice crystal-free freezing up to 15 µm in depth using sandwich technique. • No cryo-protectant required • Suitable for suspensions and emulsions g/ g, freeze drying y g or • Simpliﬁes the following freeze fracturing/etching, freeze substitution procedure ure 4 ❙❘ Freeze Etching/Freeze Fracture t BAF060 High-end freeze etching unit for precise freeze reeze fracturing with subsequent high resolution coating. • Contamination and artefact free preparation • Short preparation time with load-lock vacuum transfer • E-beam coating with variable angles and rotary shadowing • Compatible with VCT100 vacuum cryo transfer system MED020 FF Compact but modular table top freeze etching system with subsequent sputtering or e-beam evaporation coating. • Separate chambers for freeze fracturing and for coating • Precise cuts with computer controlled plane-parallel microtome ttering or ee-beam beam evaporation • High resolution coating by sputtering • Compatible with VCT100 vacuum cryo transfer system ❙❘ Cryo Transfer VCT100 Versatile transfer system for contamination free cross link of sample preparation with analysis. • Contamination freee transfer for room temperature or cryo samples • Minimum load on analysis tool ysis tool maintained • Resolution of analysis aration and analysis • Independent preparation tion of same specimen • Repeated preparation ❙❘ Coating MED020 Modular bench top high resolution coating system for multiple preparation process. • Wide variety of modules for different kinds of applications • Clean vacuum due to the turbo molecular pumping system and optional cold-trap • Universal control unit for coating, vacuum measurement and pumping system CED030 Compact bench top carbon evaporation device for TEM/EDX coating. • Cost effective production of carbon ﬁlms • Optional quartz thickness monitor available • Uniform cohesive carbon ﬁlms • Simple operation, easy and cleann loading QSG100 Quartz crystal ﬁlm thickness and rate te monitor for coating control. • Graphic user interface for easy and safe operation by use of touch screen monitor • Compact unit with integrated power supply and oscillator • High resolution 0.1 nm (C)/0.01 nm (Au) • Integrated logic interface for functional process remote control • Integrated data logger for coating thickness and rate ammable recipes • Integrated library for self-programmable Integrated periodic system of the e • elements for fast and easy programming amming ❙❘ Drying MED020 FD Freeze drying unit for reproducible drying, optionally with subsequent metal or carbon coating. • Exact temperature control of specimen cold stage • Contamination free transfer of specimens through counter ﬂow loading device Meissner trap g p • Protection against contamination based on integrated • Optional subsequent sputtering, evaporation or e-beam coating SCD500 High vacuum sputter device for high resolution FE-SEM coating. or oil-free • Diaphragm and turbo molecular pump for vacuum integrated into the housing • Simple user friendly operation • Suitable for various metal targets • Extensible for cryo applications with VCT100 vacuum cryo transfer system SCD005/SCD050 Cool sputtering devices for universal SEM coating. coating Fine grained ﬁ lms • • Water cooled sputter head ce • Pre-etching for specimen surface cleaning • Wide variety of accessories for universal applications ion • Easy and safe one button operation CPD030 Critical point drying device for biological ogical specimens. • Low CO2 consumption em • Automatic cooling/heating system Excellent visual access • • Wide selection of accessories 5 ❙❘ Solid State Technology ogy Leica EM TXP Target preparation device for milling, sawing, grinding and polishing. • Accurate location and preparation of microtargets • In-situ stereomicroscope observationn • Automatic process control to producee a mirror-like surface quality TIC020 Slope cutting with the triple ion beam cutter allows accurate and efﬁcient site speciﬁc sample preparation for SEM analysis. • Cut high quality cross sections from almost any material • Prepare big samples up to 50 x 50 x 10 mm eparation required • Minimal mechanical pre-preparation • High milling rate, broad andd deep cuts, using triple ion source RES101 Fully computer controlled all in one ion milling system with the highestt llevell off user ﬂexibility. ibilit • Variable milling angle from 0° to 90° nent high vacuum • Load-lock system for permanent • Variable ion energy for high and low energy milling processess • Built in CCD camera for sample observation RES120 SEM controlled broad ion beam milling/etching hing system for site speciﬁc sample preparation. n. • Ion beam milling with in-situ SEM observation • Preparation progress control via SE, BSE, and TE detection • Process evaluation via high resolution SEM 6 EM Sample Preparation – Industrial Samples Ultramicrotomy Leica EM UC6 Leica EM FC6 Leica EM TRIM2 Leica EM KMR2 Leica EM RAPID Cryo Fixation Leica EM PACT2/EM RTS HPM100 Leica EM CPC Leica EM MM80 E Freeze Etching/Freeze Fracture BAF060 MED020 FF Cryo Transfer VCT100 Coating MED020 SCD500 SCE005/SCD050 CED030 QSG100 Solid State Technology Leica EM TXP TIC020 RES101 RES120 7 Leica Microsystems – the brand for outstanding products • Life Science Research Division Leica Microsystems’ Life Science Research Division supports the imaging needs of the scientific community with advanced innovation and technical expertise for the visualization, measurement and analysis of microstructures. Our strong focus on understanding scientific applications puts Leica Microsystems’ customers at the leading edge of science. • Industry Division The Leica Microsystems Industry Division’s focus is to support customers’ pursuit of the highest quality end result by providing the best and most innovative imaging systems for their needs to see, measure and analyze the microstructures in routine and research industrial applications, in materials science and quality control, in forensic science investigations, and educational applications. • Biosystems Division The Biosystems Division of Leica Microsystems brings histopathology labs and researchers the highest-quality, most comprehensive product range. From patient to pathologist, the range includes the ideal product for each histology step and high-productivity workflow solutions for the entire lab. With complete histology systems featuring innovative automation and Novocastra™ reagents, the Biosystems Division creates better patient care through rapid turnaround, diagnostic confidence and close customer collaboration. • Surgical Division The Leica Microsystems Surgical Division’s focus is to partner with and support micro-surgeons and their care of patients with the highest-quality, most innovative surgical microscope technology today and into the future. Leica Microsystems’ mission is to be the world’s first-choice provider of innovative solutions to our customers’ needs for vision, measurement and analysis of micro-structures. Leica, the leading brand for microscopes and scientific instruments, developed from five brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and Cambridge Instruments. Yet Leica symbolizes innovation as well as tradition. 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