Micro-Sample Quantification Laboratory essentials, NanoDrop Products from .com labtech

Laboratory essentials, NanoDrop Products from
labtech.com
Micro-Sample Quantification
NanoDrop Products
Why NanoDrop?
Labtech International Ltd
2 Birch House, Brambleside, Bellbrook Industrial Estate, Uckfield, TN22 1QQ.
Tel: 01825 744 690 Fax: 01825 766 492 Email: [email protected] Web: www.labtech.com
Ver1: 29/11/12
NanoDrop® Lite
Basic Microvolume UV-Vis Spectrophotometer for simple sample analysis
delivered in compact footprint
The NanoDrop Lite uses the same sample retention system that has become a hallmark of NanoDrop
instruments. Whilst it is designed with fewer features than the NanoDrop 2000 and NanoDrop 8000
series, the NanoDrop Lite delivers where it counts: rapid, accurate and reproducible microvolume
measurements without the need for dilutions.
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Measures nucleic acid concentration at 260 nm and purity
using the 260/280 ratio
Measures purified protein concentration at 280 nm
Employs the unique NanoDrop microvolume sampling technology
Delivers the accuracy and reproducibility expected from NanoDrop instruments
Uses built-in controls and software - no computers required
Offers an optional printer for cryogenic labels
With its compact design, built-in controls and software, the NanoDrop Lite is small enough to fit on any
benchtop, but powerful enough to provide routine measurements for life science workflows.
Ordering Information:
Part Code
Description
ND-NDL-UK
NanoDrop Lite low volume spectrophotometer
Labtech International Ltd
2 Birch House, Brambleside, Bellbrook Industrial Estate, Uckfield, TN22 1QQ.
Tel: 01825 744 690 Fax: 01825 766 492 Email: [email protected] Web: www.labtech.com
Ver1: 29/11/12
NanoDrop® Lite Specifications
*SD of 10 individual measurements at 0.74 Abs
**Absorbance expressed at Abs/mm measured at 25°C
Ver1: 29/11/12
NanoDrop® ND-2000
ND-2000 Microvolume UV-Vis Spectrophotometer for Nucleic Acid and Protein
Quantification
The Thermo Scientific NanoDrop 2000 is the only micro-volume spectrophotometer with a patented
sample retention technology that allows for sample volumes as small as 0.5 µL.
It offers:
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Direct, easy measurements in less than 5
seconds – just pipette & wipe
Full spectral output
Measures DNA, RNA (A260) and Protein
(A280) concentrations and sample purity
(260/280 and 260/230 ratios)
Large concentration range (2 ng/µL –
15,000 ng/µL dsDNA) without dilutions
Pre-configured methods for common
applications such as Nucleic Acid, Protein
A280, Microarray, Proteins & Labels,
Bradford, BCA, Lowry and Pierce 660nm –
and more
User-friendly software that includes
Custom Methods and data export
capabilities
Simple self calibration check using control
fluid – no instrument adjustments
Low-cost operation – no plates or other
consumables
Ordering Information:
Part Code
Description
ND2000(UK)
NanoDrop ND-2000 low volume spectrophotometer incl. operating software
Labtech International Ltd
2 Birch House, Brambleside, Bellbrook Industrial Estate, Uckfield, TN22 1QQ.
Tel: 01825 744 690 Fax: 01825 766 492 Email: [email protected] Web: www.labtech.com
Ver1: 29/11/12
NanoDrop® ND-2000c
ND-2000c Dual-mode UV-Vis Spectrophotometer with Micro-volume AND
Cuvette Capability for Nucleic Acid and Protein Quantification
The Thermo Scientific NanoDrop 2000c is the
only micro-volume spectrophotometer that
integrates a patented sample retention
technology for samples as small as 0.5 µL and a
cuvette capability.
It offers:
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All the features of the ND-2000
Spectrophotometer – plus cuvette mode
Pre-configured methods for all common
Nucleic Acid and Protein Concentration and
Purity measurements, in both modes
Custom Methods capability, in both modes
Cell cultures (OD600)
Kinetic studies (time and 37°C temp) with
stirring, in cuvette mode
Extended concentration range (lower limit
0.4 ng/µL dsDNA) in cuvette mode
Accepts standard cuvettes
Ordering Information:
Micro-volume pedestal
Part Code
Description
ND2000C(UK)
NanoDrop ND-2000c low volume spectrophotometer with added cuvette
capability incl. operating software
Labtech International Ltd
2 Birch House, Brambleside, Bellbrook Industrial Estate, Uckfield, TN22 1QQ.
Tel: 01825 744 690 Fax: 01825 766 492 Email: [email protected] Web: www.labtech.com
Ver1: 29/11/12
NanoDrop® ND-2000 | ND-2000c Specifications
Ver1: 29/11/12
NanoDrop® ND-2000 | ND-2000c
The patented sample retention system used by
the Thermo Scientific NanoDrop 2000 and
NanoDrop™ 2000c allows for the analysis of 0.5
µl - 2.0 µl samples, without the need for cuvettes
or capillaries.
With the arm open, a sample is pipetted directly
onto the pedestal.
After the arm is closed, a sample column is
formed.
The pedestal then moves to automatically adjust
for an optimal path length (0.05 mm - 1 mm).
When the measurement is complete, the surfaces
are simply wiped with a lint-free lab wipe before
going on to the next sample.
Cuvette Measurements
The NanoDrop 2000c model includes a built-in
cuvette capability. Most
common cuvettes and micro-cuvettes may be
used with this instrument
(8.5 mm z-height and 2 mm beam).
Labtech International Ltd
2 Birch House, Brambleside, Bellbrook Industrial Estate, Uckfield, TN22 1QQ.
Tel: 01825 744 690 Fax: 01825 766 492 Email: [email protected] Web: www.labtech.com
Ver1: 29/11/12
NanoDrop® ND-8000
Sample position illuminator
Multi-sample loading
Sample position measurement
ND-8000 Multi-Sample UV-Vis Micro-volume Spectrophotometer
With the Thermo Scientific NanoDrop 8000, you can measure more samples in less time with the same
reliability and patented sample retention technology of the popular ND-2000 .
It offers:
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Measure nucleic acid and protein
concentration of samples as small as 1
µL
Direct, easy measurements from tubes
or plates
Single sample mode or up to eight
samples at a time
Analysis of 96 samples in less than 6
minutes
Full spectral output
Calculates sample purity ratios
(260/280 and 260/230 ratios)
Pre-configured methods for common
applications such as Nucleic Acid,
Protein A280, Microarray, Proteins &
Labels, Bradford, BCA, Lowry and
Pierce 660nm– and more
Intuitive and flexible software for
creating custom methods
and exporting data
No consumables
Sample Position Illuminator which
increases efficiency and reduces error
by keeping track of the samples to be
measured
Perfect for core labs, bioprocessing QC/manufacturing environments, biorepositories and molecular
diagnostics labs.
Ordering Information
Part Code
Description
ND8000(UK)
NanoDrop ND-8000 8 channel low volume spectrophotometer incl. operating
software and sample illumination.
Labtech International Ltd
2 Birch House, Brambleside, Bellbrook Industrial Estate, Uckfield, TN22 1QQ.
Tel: 01825 744 690 Fax: 01825 766 492 Email: [email protected] Web: www.labtech.com
Ver1: 29/11/12
NanoDrop® ND-8000 Specifications
Ver1: 29/11/12
NanoDrop® 3300 Fluorospectrometer
Sample position illuminator
Multi-sample loading
Micro-volume Full Spectrophotometer
Using our patented sample retention technology, the versatile and cost-effective Thermo Scientific
NanoDrop 3300 Fluorospectrometer makes broad spectrum fluorescence analysis quick and easy.
It offers:
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Sample size as small as 1 µl
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Mass detection limit more than one order
of magnitude lower than conventional
fluorometers
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UV, Blue and White LED excitation,
covering a broad wavelength range
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No filter changes, no monochromator
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Ability to analyze multiple emission
profiles from a single sample
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Simplicity of use – just pipette and wipe
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User-friendly software
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Ability to run diverse applications multiple fluorophores, Molecular Beacon
Probes, FRET and more
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A cost-effective, unique alternative to high-end fluorometers or complicated lab-built systems with
microscopes and CCD cameras
Perfect for any research and teaching labs interested in fluorescence.
Ordering information
Part Code
Description
ND3300
NanoDrop 3300 low volume fluorospectrometer incl. operating software
Labtech International Ltd
2 Birch House, Brambleside, Bellbrook Industrial Estate, Uckfield, TN22 1QQ.
Tel: 01825 744 690 Fax: 01825 766 492 Email: [email protected] Web: www.labtech.com
Ver1: 29/11/12
NanoDrop® 3300 Specifications
Ver1: 29/11/12
Laboratory essentials, NanoDrop Products
distributed exclusively by labtech.com
What our customers say...
“We’ve been running qPCR training courses for many years and in our workshops we emphasise that the
NanoDrop is an invaluable piece of equipment in the quality control evaluation of nucleic acids. The most robust
qPCR data are achieved from good quality template, and we use the NanoDrop routinely as part of the QC in our
workflow. Unlike other methods , the NanoDrop permits visualisation of the absorbance spectrum, which provides
invaluable information about contaminants in a sample, for example a shift in the peak from 260 nm indicates the
presence of substances other than nucleic acid. We also supply qPCR standards of known copy number
commercially. Accurate quantification is important to us and we use the absorbance at 260 nm provided by the
NanoDrop to calculate the number of copies in new batches. We recently validated the accuracy of the NanoDrop
method by absolute quantification using digital droplet PCR and found excellent agreement between the two
methods. We have no hesitation in recommending the NanoDrop as an essential piece of equipment in any lab
doing qPCR.” Patricia De Winter qStandard
“Our lab recently purchased the Nanodrop ND 2000 Spectrophotometer from Labtech International following
recommendation from colleagues in a laboratory in Cambridge who used the ND 1000 and were very enthusiastic
about it.
Following its installation in the laboratory I have received positive feedback from various users. The
instrument is very simple to use, providing a very rapid measurement of DNA, RNA and proteins on a sample
volume as low as 0.5µL (which we have found to be a great benefit). It also gives the possibility to customise
methods, design reports and export data. In addition to micro-volume measurements, kinetics (time and
temperature studies) and cell cultures analysis (OD 600) can also be performed. The Nanodrop provides a large
concentration range compared to a conventional spectrophotometer without the requirement for preparation of
dilutions.
In summary, all members of our laboratory are more than happy with the Nanodrop ND2000 and it is
considered to be of great benefit for our daily lab procedures.”Matthew Golder – Institute of Comparative
Medicine University of Glasgow
“The ND2000 has allowed us to produce accurate and reproducible measurements of protein concentration, using
only a minimal amount of sample and reagents. It is simple and relatively quick to use, whilst having a considerable
depth of options and methods. Using the ND2000 we have been able to accurately measure protein concentration
in complex biological samples, purified antibodies and fluorescent dye-antibody conjugates, also peptide
concentration. The ND2000 has now replaced our old spectrophotometer plate reader.” Ludmila Bozhenok
Oxford BioTherapeutics Ltd
“The NanoDrop has made quantification much quicker, no more cleaning of cuvettes and using large amounts of
precious samples.
We have only had it in the lab for a few weeks and already I have noticed it being used a lot more heavily, because
of the speed and low sample volume,than our old spectrophotometer ever was. Most importantly it is so simple to
use and keep clean, just wish we had bought one years ago!” Matt Hall Marine Biological Association
“I’m just in the lab using the NanoDrop now, and I’m VERY pleased with it!!! It’s so nice to be looking at results that
I know I can rely on!” Dr Fiona McRonald Liverpool University
“There are two types of molecular biologists, those that have a NanoDrop and those that want one.” Dr Malcolm
Campbell
Labtech International Ltd
2 Birch House, Brambleside, Bellbrook Industrial Estate, Uckfield, TN22 1QQ.
Tel: 01825 744 690 Fax: 01825 766 492 Email: [email protected] Web: www.labtech.com
Ver1: 29/11/12