Waters Oasis® MCX Sample Extraction Products Drug monitoring achieved with the highest selectivity, sensitivity and speed. The better way to monitor all drugs and extract basic drugs from urine, plasma and serum. Since 1996 Oasis® sample extraction products have been setting new standards in solid-phase extraction. The Oasis® HLB products freed you from using stopcocks and pH constraint. Life in the bioanalysis lab has been made simpler and more efficient. Cation eXchange/ reversed-phase sorbent enables the retention of acidic, neutral and basic drugs. The Oasis® MCX sorbent is ideal for extracting the NIDA 5**: amphetamines, opiates, cocaine, phencyclidine and tetrahydrocannibinol. Now you can achieve even higher selectivity and sensitivity for extracting basic drugs with the new member of the Oasis® family. The Oasis® MCX sorbent* has all the benefits of the HLB sorbent but with a new hook; strong sulfonic (HSO3) groups have been bonded onto the poly(divinylbenzene-co-N-polyvinylpyrrolidone) copolymer to give a cationexchange functionalities. The Mixed-mode Most important is the selective retention of basic drugs by one type of cation-exchange groups at a known and tightly controlled ion extraction capacity (1 meg/gram). There are no silanol groups to complicate the mode of retention or method development. This novel water-wettable polymeric sorbent is stable from pH 0 to 14, making method development simple and fast. * patent-pending ** NIDA is the National Institute of Drug Abuse High confidence in results Figure 1: Methadone extraction with the highest confidence. The high level of reproducibility combined with high selectivity for bases assures you of the same results across subjects. Figure 1 shows high recovery and reproducibility of methadone from human urine using the Oasis® MCX cartridges. The overall RSD was 3% from three different human urine samples, which is lower than the intra-subject variability observed for many traditional sorbents. These results emphasize the high confidence you can have in your drug monitoring data; the concern about false positives or false negatives is no longer an issue. % Recovery 100 1.5% RSD 0.3% RSD 0.5% RSD 3.0% RSD % Recovery 80 Methadone was extracted from the urine of three different subjects using Oasis® MCX cartridges, 3 cc/60 mg, by the method shown in figure 2. 60 40 20 0 Subject A Subject B Subject C Inter-subject (0.5 µg/L) Batch-to-batch reproducibility Enhanced selectivity for basic drugs The Oasis® MCX sorbent is designed to extract basic drugs from complex matrices such as whole blood, urine, serum, or plasma. The water-wettable Oasis® MCX sorbent can be used for human urine extractions without conditioning. Just remove the cartridge or plate from the package and apply the sample. As shown in Figure 2, one protocol with minimum wash steps gives extractions fast enough to keep pace with your analysis system. Excellent results were obtained for several basic drugs using this protocol (Figure 4). If additional cleanup is required for your gas chromatography, LC/MS or HPLC analysis, none of the pH constraints that limit silica-based sorbents apply. Optimizing the method is Consistent and continuous supply of Oasis® MCX sorbent is guaranteed because we manufacture it in Waters own ISO 9002certified plant under cGMP standard. The starting Oasis® HLB sorbent is quality controlled by a set of stringent test. Each batch of the final Oasis® MCX sorbent is checked again for quality control by a recovery tests using acidic, neutral and basic compounds. As a result of the careful process control and stringent quality control test, reproducibility is certified from batch-to-batch. The Certificate of analysis (COA) that is included in every package gives the product specifications and sorbent batch test results. The same standardsetting batch-to-batch reproducibility you have with Oasis® HLB products are now guaranteed with Oasis® MCX sample extraction cartridges and 96-well plates. simple and straightforward. The polymeric Oasis® MCX sorbent has no pH constraints, so the optimum pH for getting the cleanest extract can be used. All this can be done without stopcocks on your vacuum manifold. High throughput methods can be transferred to the Oasis® MCX 96-well extraction plate. High reproducibility and recovery The Oasis® MCX products give you a fast, efficient and reproducible extraction of basic drugs from complex matrices. Routine recoveries greater than 85% with RSDs less than 5% can be achieved across a broad range of basic drugs. Silica-based sorbents with mixed-mode functionalities do not perform the same for all basic drugs, some recoveries are acceptable, others are less than 66% with RSDs greater than 10%. (Table 1). Table 1: Competitor comparison to Oasis® MCX cartridges for human urine extraction Oasis® MCX 60 mg/3 cc Compound C8/SCX* Brand A 300 mg/3 cc C8/SCX* Brand B 200 mg/3 cc Concentration (µg/mL) Recovery (%) RSD (%) Recovery (%) RSD (%) Recovery (%) RSD (%) 0.5 97.2 0.3 53.8 2.4 56.1 13.4 0.2 93.2 0.7 55.9 3.2 65.2 12.2 0.4 97.7 0.5 88.7 3.2 87.6 1.1 O N Methadone Methadone Metabolite** N+ CH 3 Propanolol O OH N H CH 3 Samples were extracted by the method in Figure 2. n =3; the samples were analyzed by HPLC using SymmetryShield™ columns. * Mixed mode Cation eXchange (C8/SCX) ** EDDP is the metabolite Universal sorbent With the new Oasis® MCX sorbent you are not limited to extracting only basic drugs. You can isolate a wide range of acidic, basic and neutral compounds with one sorbent. Acidic and neutral drugs are retained by the same mechanism as on the Oasis® HLB sorbent, giving high and reproducible recoveries for drugs covering a wide polarity range even if the bed runs dry (Table 2). The polar neutral drug acetaminophen was extracted with high recovery (103%) and low variability (RSD 0.55%). Table 2: High recovery of acidic, neutral and basic drugs from plasma in two fractions Compound H N Acetominophen H3C Concentration (µg/mL) Drug Type Recovery (%) RSD (%) Elute A 2 µg/mL Polar Neutral 103.4 0.55 Elute A 2 µg/mL Weak Acid 99.4 1.05 Elute B 4 µg/mL Base 96.8 1.35 Elute B 4 µg/mL Base 90.4 4.01 OH O O Barbital Fraction H N O NH O CH 3 Amphetamine NH 2 H N Metamphentamine Samples were extracted by the method in Figure 3, evaporation was not required. Screening and fractionation The reversed-phase and cation exchange functionalities on the sorbent allow samples to be fractionated into the combined acidic and neutral drug fraction (Figure 3 Elute A) and a basic drug fraction (Figure 3 Elute B). The Oasis® MCX sorbent can be used for drug screening by testing the methanol fraction for the presence of acidic and neutral drugs and the ammonium hydroxide-methanol fraction for basic drugs. The universal nature of this novel polymeric mixed-mode sorbent extends its use to the subsequent stages of drug testing. The Oasis® MCX sorbent can be used to confirm the presence of a drug and even for determining its concentration in biofluid, when required. One sorbent and often one method can be applied for all stages of drug testing. Figure 2: Oasis® MCX methods for extraction of basic drugs from human urine Figure 3: Oasis® MCX method for extractions of neutral, acidic and basic drugs Load: 3 mL spiked and acidified urine Condition/Equilibrate: 1 mL methanol/1 mL water Wash 1: 2 mL 0.1N HCI Load: 1 mL spiked and acidified plasma Wash 2: 2 mL methanol Wash 1: 1 mL 0.1N HCI Elute: 2 mL 5% ammonium hydroxide in methanol Elute A: 1 mL methanol Evaporate and Reconstitute in 300 µL 20% methanol in water Elute B: 1 mL 5% ammonium hydroxide in methanol For further cleanup an additional wash step may be necessary Evaporate and Reconstitute Figure 4: High recoveries of basic drugs on Oasis® MCX sorbent without preconditioning %Recovery Methadone % RSD N O O 0.2 µg/mL 1.5% 0.08 µg/mL 2.7% Oxprenolol 0.08 µg/mL 3.2% Metoprolol 0.08 µg/mL 3.4% Verapamil 4.5 µg/mL 0.2% Nor-Verapamil 3.4 µg/mL 0.5% 0.12 µg/mL 1.2% Codeine-6-glucronide 0.12 µg/mL 1.5% Ranitidine 0.24 µg/mL 2.6% Methadone metabolite (EDDP) Bonin, Cheng, Woods 60 80 100 Method given in Figure 2 with n = 3 or 4 (Human urine samples) N OCH 3 OCH 3 Metoprolol Codeine CH 3O CH 3 N+ O OH N H CH 3 O H NCH 3 HO CH 3O Estazolam Verapamil N N CH 3O N Cl CN Codeine-6-glucronide CH 3 N CH 3O CH 3O OCH 3 O Nor-Verapamil O OH N H CH 3 CH 3O CH 3O CN H N NCH 3 O OH O OH OH Propranolol H OCH 3 HOOC CH 3 40 CN CH 3O OCH 3 3.3% Codeine 20 CH 3 CH 3 CH 3O N 6.7 µg/mL 0 N H OH 1.0% Propranolol Verapamil-Methoxy Verapamil-Methoxy CH 3 2.7% 0.04 µg/mL Estazolam Oxprenolol O 0.01 µg/mL Methadone metabolite (EDDP) Methadone Ranitidine N OCH 3 OCH 3 O S N N NO2 Available in two particle sizes for biofluid samples Table 3: Oasis® MCX particle sizes Sample The Oasis® MCX sorbent is designed to extract drugs from all matrices. The two available particle sizes (30 µm and 60 µm) of the Oasis® MCX sorbent allow you to select the appropriate product based on the viscosity and turbidity of your sample (Table 3). For extractions from most plasma, serum and human urine choose the 30 µm sorbent. For more viscous samples, such as animal urine, or ?????? samples, excellent flow can be achieved using the 60 µm (LP) sorbent. 30 µm Plasma ✔ Serum ✔ 60 µm (LP)* Whole blood ✔ — cadaver ✔ — human/animal Urine — human ✔ — dog ✔ ✔ ✔ — horse * LP refers to the 60 µm particle size. Ordering Information Description Quantity Part Numbers ® 1/pkg 186000259 ® 3/pkg 186000260 ® 1/pkg 186000248 ® 3/pkg 186000249 ® 1/pkg 186000250 ® Oasis MCX Extraction Plate 30 mg LP/96-well 3/pkg 186000251 Oasis® MCX Extraction Cartridges 1cc/30 mg Oasis MCX Extraction Plate 10 mg/96-well Oasis MCX Extraction Plate 10 mg/96-well Oasis MCX Extraction Plate 30 mg/96-well Oasis MCX Extraction Plate 30 mg/96-well Oasis MCX Extraction Plate 30 mg LP/96-well 100/box 186000252 ® 100/box 186000254 ® 100/box 186000253 ® 30/box 186000256 ® 30/box 186000255 ® 50/box Inquire Oasis MCX Extraction Cartridges 3cc/60 mg Oasis MCX Extraction Cartridges 3cc/60 mg LP Oasis MCX Extraction Cartridges 6cc/150 mg Oasis MCX Extraction Cartridges 6cc/150 mg LP Oasis MCX Extraction Cartridges Vac RC/60 mg LP For other configurations, please inquire. 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