Waters Oasis® MCX
Sample Extraction
Products
Drug monitoring achieved
with the highest selectivity,
sensitivity and speed.
The better way to monitor all
drugs and extract basic drugs
from urine, plasma and serum.
Since 1996 Oasis® sample extraction products
have been setting new standards in solid-phase
extraction. The Oasis® HLB products freed you
from using stopcocks and pH constraint. Life in
the bioanalysis lab has been made simpler
and more efficient.
Cation eXchange/ reversed-phase sorbent
enables the retention of acidic, neutral and
basic drugs. The Oasis® MCX sorbent is ideal
for extracting the NIDA 5**: amphetamines,
opiates, cocaine, phencyclidine and
tetrahydrocannibinol.
Now you can achieve even higher selectivity
and sensitivity for extracting basic drugs with
the new member of the Oasis® family. The
Oasis® MCX sorbent* has all the benefits of
the HLB sorbent but with a new hook; strong
sulfonic (HSO3) groups have been bonded
onto the poly(divinylbenzene-co-N-polyvinylpyrrolidone) copolymer to give a cationexchange functionalities. The Mixed-mode
Most important is the selective retention of
basic drugs by one type of cation-exchange
groups at a known and tightly controlled ion
extraction capacity (1 meg/gram). There are
no silanol groups to complicate the mode of
retention or method development. This novel
water-wettable polymeric sorbent is stable from
pH 0 to 14, making method development
simple and fast.
* patent-pending
** NIDA is the National Institute of Drug Abuse
High confidence in results
Figure 1: Methadone extraction with the highest confidence.
The high level of reproducibility combined with
high selectivity for bases assures you of the
same results across subjects. Figure 1 shows
high recovery and reproducibility of
methadone from human urine using the Oasis®
MCX cartridges. The overall RSD was 3% from
three different human urine samples, which is
lower than the intra-subject variability observed
for many traditional sorbents. These results
emphasize the high confidence you can have
in your drug monitoring data; the concern
about false positives or false negatives is no
longer an issue.
% Recovery
100
1.5% RSD 0.3% RSD 0.5% RSD 3.0% RSD
% Recovery
80
Methadone was extracted
from the urine of three different
subjects using Oasis® MCX
cartridges, 3 cc/60 mg, by
the method shown in figure 2.
60
40
20
0
Subject A
Subject B
Subject C
Inter-subject
(0.5 µg/L)
Batch-to-batch reproducibility
Enhanced selectivity for
basic drugs
The Oasis® MCX sorbent is designed to extract
basic drugs from complex matrices such as
whole blood, urine, serum, or plasma. The
water-wettable Oasis® MCX sorbent can be
used for human urine extractions without
conditioning. Just remove the cartridge or plate
from the package and apply the sample. As
shown in Figure 2, one protocol with minimum
wash steps gives extractions fast enough to
keep pace with your analysis system. Excellent
results were obtained for several basic drugs
using this protocol (Figure 4).
If additional cleanup is required for your gas
chromatography, LC/MS or HPLC analysis,
none of the pH constraints that limit silica-based
sorbents apply. Optimizing the method is
Consistent and continuous supply of Oasis®
MCX sorbent is guaranteed because we
manufacture it in Waters own ISO 9002certified plant under cGMP standard. The
starting Oasis® HLB sorbent is quality
controlled by a set of stringent test. Each batch
of the final Oasis® MCX sorbent is checked
again for quality control by a recovery tests
using acidic, neutral and basic compounds.
As a result of the careful process control and
stringent quality control test, reproducibility is
certified from batch-to-batch. The Certificate of
analysis (COA) that is included in every
package gives the product specifications and
sorbent batch test results. The same standardsetting batch-to-batch reproducibility you have
with Oasis® HLB products are now
guaranteed with Oasis® MCX sample
extraction cartridges and 96-well plates.
simple and straightforward. The polymeric
Oasis® MCX sorbent has no pH constraints, so
the optimum pH for getting the cleanest extract
can be used. All this can be done without
stopcocks on your vacuum manifold. High
throughput methods can be transferred to
the Oasis® MCX 96-well extraction plate.
High reproducibility and recovery
The Oasis® MCX products give you a fast,
efficient and reproducible extraction of basic
drugs from complex matrices. Routine
recoveries greater than 85% with RSDs less
than 5% can be achieved across a broad
range of basic drugs. Silica-based sorbents
with mixed-mode functionalities do not perform
the same for all basic drugs, some recoveries
are acceptable, others are less than 66% with
RSDs greater than 10%. (Table 1).
Table 1: Competitor comparison to Oasis® MCX cartridges for human urine extraction
Oasis® MCX
60 mg/3 cc
Compound
C8/SCX*
Brand A
300 mg/3 cc
C8/SCX*
Brand B
200 mg/3 cc
Concentration
(µg/mL)
Recovery
(%)
RSD
(%)
Recovery
(%)
RSD
(%)
Recovery
(%)
RSD
(%)
0.5
97.2
0.3
53.8
2.4
56.1
13.4
0.2
93.2
0.7
55.9
3.2
65.2
12.2
0.4
97.7
0.5
88.7
3.2
87.6
1.1
O
N
Methadone
Methadone
Metabolite**
N+
CH 3
Propanolol
O
OH
N
H
CH 3
Samples were extracted by the method in Figure 2. n =3; the samples were analyzed by HPLC using SymmetryShield™ columns.
* Mixed mode Cation eXchange (C8/SCX) ** EDDP is the metabolite
Universal sorbent
With the new Oasis® MCX sorbent you are
not limited to extracting only basic drugs. You
can isolate a wide range of acidic, basic
and neutral compounds with one sorbent.
Acidic and neutral drugs are retained by the
same mechanism as on the Oasis® HLB
sorbent, giving high and reproducible
recoveries for drugs covering a wide polarity
range even if the bed runs dry (Table 2). The
polar neutral drug acetaminophen was
extracted with high recovery (103%) and low
variability (RSD 0.55%).
Table 2: High recovery of acidic, neutral and basic drugs from plasma in two fractions
Compound
H
N
Acetominophen
H3C
Concentration
(µg/mL)
Drug Type
Recovery
(%)
RSD
(%)
Elute A
2 µg/mL
Polar Neutral
103.4
0.55
Elute A
2 µg/mL
Weak Acid
99.4
1.05
Elute B
4 µg/mL
Base
96.8
1.35
Elute B
4 µg/mL
Base
90.4
4.01
OH
O
O
Barbital
Fraction
H
N
O
NH
O
CH 3
Amphetamine
NH 2
H
N
Metamphentamine
Samples were extracted by the method in Figure 3, evaporation was not required.
Screening and fractionation
The reversed-phase and cation exchange
functionalities on the sorbent allow samples to
be fractionated into the combined acidic and
neutral drug fraction (Figure 3 Elute A) and a
basic drug fraction (Figure 3 Elute B). The
Oasis® MCX sorbent can be used for drug
screening by testing the methanol fraction for
the presence of acidic and neutral drugs and
the ammonium hydroxide-methanol fraction for
basic drugs. The universal nature of this novel
polymeric mixed-mode sorbent extends its use
to the subsequent stages of drug testing. The
Oasis® MCX sorbent can be used to confirm
the presence of a drug and even for
determining its concentration in biofluid, when
required. One sorbent and often one method
can be applied for all stages of drug testing.
Figure 2: Oasis® MCX methods
for extraction of basic drugs
from human urine
Figure 3: Oasis® MCX method
for extractions of neutral,
acidic and basic drugs
Load: 3 mL spiked
and acidified urine
Condition/Equilibrate:
1 mL methanol/1 mL water
Wash 1:
2 mL 0.1N HCI
Load: 1 mL spiked
and acidified plasma
Wash 2:
2 mL methanol
Wash 1:
1 mL 0.1N HCI
Elute: 2 mL 5% ammonium
hydroxide in methanol
Elute A:
1 mL methanol
Evaporate and Reconstitute in
300 µL 20% methanol in water
Elute B: 1 mL 5%
ammonium hydroxide in methanol
For further cleanup an additional wash
step may be necessary
Evaporate and Reconstitute
Figure 4: High recoveries of basic drugs on Oasis® MCX sorbent without preconditioning
%Recovery
Methadone
% RSD
N
O
O
0.2 µg/mL
1.5%
0.08 µg/mL
2.7%
Oxprenolol
0.08 µg/mL
3.2%
Metoprolol
0.08 µg/mL
3.4%
Verapamil
4.5 µg/mL
0.2%
Nor-Verapamil
3.4 µg/mL
0.5%
0.12 µg/mL
1.2%
Codeine-6-glucronide
0.12 µg/mL
1.5%
Ranitidine
0.24 µg/mL
2.6%
Methadone metabolite (EDDP)
Bonin, Cheng, Woods
60
80
100
Method given in Figure 2 with n = 3 or 4
(Human urine samples)
N
OCH 3
OCH 3
Metoprolol
Codeine
CH 3O
CH 3
N+
O
OH
N
H
CH 3
O
H
NCH 3
HO
CH 3O
Estazolam
Verapamil
N
N
CH 3O
N
Cl
CN
Codeine-6-glucronide
CH 3
N
CH 3O
CH 3O
OCH 3
O
Nor-Verapamil
O
OH
N
H
CH 3
CH 3O
CH 3O
CN
H
N
NCH 3
O
OH
O
OH
OH
Propranolol
H
OCH 3
HOOC
CH 3
40
CN
CH 3O
OCH 3
3.3%
Codeine
20
CH 3
CH 3
CH 3O
N
6.7 µg/mL
0
N
H
OH
1.0%
Propranolol
Verapamil-Methoxy
Verapamil-Methoxy
CH 3
2.7%
0.04 µg/mL
Estazolam
Oxprenolol
O
0.01 µg/mL
Methadone metabolite (EDDP)
Methadone
Ranitidine
N
OCH 3
OCH 3
O
S
N
N
NO2
Available in two particle
sizes for biofluid samples
Table 3: Oasis® MCX particle sizes
Sample
The Oasis® MCX sorbent is designed to extract
drugs from all matrices. The two available
particle sizes (30 µm and 60 µm) of the Oasis®
MCX sorbent allow you to select the
appropriate product based on the viscosity and
turbidity of your sample (Table 3). For
extractions from most plasma, serum and
human urine choose the 30 µm sorbent. For
more viscous samples, such as animal urine, or
?????? samples, excellent flow can be
achieved using the 60 µm (LP) sorbent.
30 µm
Plasma
✔
Serum
✔
60 µm (LP)*
Whole blood
✔
— cadaver
✔
— human/animal
Urine
— human
✔
— dog
✔
✔
✔
— horse
* LP refers to the 60 µm particle size.
Ordering Information
Description
Quantity
Part Numbers
®
1/pkg
186000259
®
3/pkg
186000260
®
1/pkg
186000248
®
3/pkg
186000249
®
1/pkg
186000250
®
Oasis MCX Extraction Plate 30 mg LP/96-well
3/pkg
186000251
Oasis® MCX Extraction Cartridges 1cc/30 mg
Oasis MCX Extraction Plate 10 mg/96-well
Oasis MCX Extraction Plate 10 mg/96-well
Oasis MCX Extraction Plate 30 mg/96-well
Oasis MCX Extraction Plate 30 mg/96-well
Oasis MCX Extraction Plate 30 mg LP/96-well
100/box
186000252
®
100/box
186000254
®
100/box
186000253
®
30/box
186000256
®
30/box
186000255
®
50/box
Inquire
Oasis MCX Extraction Cartridges 3cc/60 mg
Oasis MCX Extraction Cartridges 3cc/60 mg LP
Oasis MCX Extraction Cartridges 6cc/150 mg
Oasis MCX Extraction Cartridges 6cc/150 mg LP
Oasis MCX Extraction Cartridges Vac RC/60 mg LP
For other configurations, please inquire.
Visit us on the Internet: http://www.waters.com
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All other countries: Waters Corporation U.S.A. 508 478 2000/800 252 4752
Waters and Oasis are trademarks of Waters Corporation.
The quality management system of Waters’ manufacturing facility, Taunton,
Massachusetts, complies with the International Standard ISO 9002 Quality
Management and Quality Assurance Standards. Waters’ quality management
system is periodically audited by the registering body to ensure compliance.
©1998 Waters Corporation, 9/98 WB071