sample
preparation
Ultra-Micro TipColumn™
Micro TipColumn,™
see page 2 to 3
Products
Page No.
Sample Preparation
Guide to Sample Preparation Products
2-6
96-Well SpinColumns™
96-Well Micro SpinColumns™
96-Well Macro SpinColumns,™
see page 2 to 3
Fast Flow-Thru DIALYZER,
see page 4-5
Ultra-Micro DispoDIALYZER™
(patented), see page 4-5
1
sample preparation
Guide to Sample Preparation Products
sample preparation
Guide to Sample Preparation Products
Single Use Disposable Chromatographic Products
Sample
Volumes (µl)
Materials &
Binding
Tip Volumes Capacity (µg/tip)
Packing Materials
min
max
volume volume
Packing Materials
Gel filtration (V0=KD)
(Sephadex 45-90 microns)
Hydrophobic
(particle size 15-20 microns)
Ion exchange (Sepharose):
Q=Quaternary Amine; SP=Sulphopropyl;
DEAE=cross-linked Diethylaminoethyl;
CM=Carboxymethyl 12mu; 300A
Hydrophillic
TIPS
G10= G25= G50= G100=
700D 5KD 30KD 100KD
Ultra-Micro TipColumn™
A wide selection of pre-packed chromatographic
materials in pipette tip formats
10
Micro TipColumn™
A wide selection of pre-packed chromatographic
materials in pipette tip formats
25
Ultra-Micro PrepTip™ (patented)
A revolutionary fritless chromatographic device.
Does separation without clogging
1
Micro PrepTip™ (patented)
A revolutionary fritless chromatographic device
Does separation without clogging
10
Macro PrepTip™ (patented)
A revolutionary fritless chromatographic device
Does separation without clogging
100
Ultra-Micro ProTip™
Contains a hydrophilic polymer material which
binds 5 to 10X more protein than silica
1
Micro ProTip™
Contains a hydrophilic polymer material which
binds 5 to 10X more protein than silica
10
Macro ProTip™
Contains a hydrophilic polymer material which
binds 5 to 10X more protein than silica
100
25
75
10
100
500
10
100
500
PP body
PE frit
300 µl
P2=
2KD
P6=
6KD
C18 C8 C4
**** **** ****
PVOH PHEA
silica CN NH2 **
***
Miscellaneous
IMAC†= (Imidodiacetate on
cross linked divinyl benzene)
Strong
Strong
Weak
Weak Activated
Anion
Q
Cation
SP
Anion
DEAE
Cation
CM
Empty Column
& Frit Size
(microns)
Charcoal Cellulose Detergent
Removal IMAC 5-10
CAS 74440-44-0 CAS 9004-34-6
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20
40
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STD
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STD
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STD
PP body
PE frit
300 µl
3 - 60
PP body
no frit
10 µl
0.5
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PP body
no frit
200 µl
1.0
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PP body
PE frit
1,000 µl
5.0
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PP body
PE frit
10 µl
0.2
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PP body
PE frit
200 µl
1.5
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PP body
PE frit
1,000 µl
9.0
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PP body, PE frits
(PE cap for
gels only) 300 µl
3 - 30
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PP body, PE frits
(PE cap for
gels only) 300 µl
5 - 60
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STD
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30 - 300
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STD
5 - 60
30 - 300
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STD
STD
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SPINCOLUMNS
Ultra-Micro SpinColumn™
10
A wide selection of packed chromatographic
materials in SpinColumn formats
25
Micro SpinColumn™
25
A wide selection of packed chromatographic
materials in SpinColumn formats
75
75
150
Macro SpinColumn™
A wide selection of packed chromatographic
materials in SpinColumn formats, 1 sample
collection tube
96-Well SpinColumns™
96-well plate for HTS sample prep,
2 sample collection plates
96-Well Micro SpinColumns™
96-Well Macro SpinColumns™
PP body
PE frit
PE caps
1,000 µl
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PP body
PE frit
25
25
75
150
300 µl
400 µl
PP=Polypropylene; PE= Polyethylene
C18, C8, C4 ****=Vydac, ** Polyvinylalcohol, *** Polyhydroxyethyl Aspartamide
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
2
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
3
sample preparation
sample preparation
Guide to Sample Preparation Products
Guide to Sample Preparation Products
DIALYSIS/ELECTRO DIALYSIS PRODUCTS
Sample Volumes (µl)
Membranes (Da or µm)
Number of
Membranes
Materials
Membranes (Da or µm)
Regenerated Cellulose MWCO (Da)
Cellulose Acetate (Aqueous/ organic)
MWCO (Da)
REUSABLE IMMERSIBLE CHAMBERS
(
Ordering information
Polycarbonate (Aqueous/organic/
autoclavable) pore size, (µm)
1K
2K
5K
10K
25K
50K
100
500
1K
2K
5K
10K
25K
50K
100K
300K
0.01
(100K)
0.05
(800K)
0.1
(1.1M)
0.6
(2.5M)
PTFE
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PTFE
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Includes chamber and 1 open-ended cap;
(membranes, stirrer plate and stir bar
sold separately)
Includes chamber and 2 caps; (membranes,
stirrer plate and stir bar sold separately)
1,500
1
PTFE
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Includes chamber with magnet and cap;
(membranes and stirrer plate sold separately)
50
1,500
2
PTFE
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Includes chamber with magnet and 2 caps;
(membranes and stirrer plate sold separately)
25
>1,500
up to six
PTFE
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Fast Flow-Thru DIALYZER
Drop-in, continuous flow, single chambered,
in-line dialysis system (4 chamber sizes available)
50
>10,000
2
PTFE
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Flow-Thru DIALYZER
Continuous flow, high surface area, in-line
dialysis system (5 chamber sizes available)
20
>10,000
1
PTFE
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5
1
PC body
PP tube
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min
max
10
5,000
1
50
5,000
SpinDIALYZER™
Drop-in, re-usable dialysis chamber
with built-in magnet (8 chamber sizes available)
10
Fast SpinDIALYZER™
Drop-in, re-usable, two-sided dialysis chamber
with built-in magnet (6 chamber sizes available)
ElectroPrep™ (patented)
Ultra-fast, single or multi-chambered dialysis
system capable of separating by size & charge
DIALYZER
Drop-in, re-usable dialysis chamber
Ten chamber sizes available)
Fast DIALYZER
Drop-in, re-usable two-sided dialysis
chamber (8 chamber sizes available)
Includes tank with seal and lid with leads;
(chambers, membranes and power supply
sold separately)
REUSABLE FLOW-THRU DIALYZERS
Includes chamber, fittings and 2 caps
(membranes, tubing and pump sold separately)
Includes 2 chambers, membranes, fittings,
clamp and allen wrench;
(sold with or without pump)
SINGLE USE DIALYSIS PRODUCTS
Ultra-Micro DispoDIALYZER™ (patented)
Micro DispoDIALYZER™ (patented)
Macro Fast DispoDIALYZER
96-Well DispoDIALYZER
5
1,000
25
100
10,000
300
1
2
1/chamber
PC body
PP head
PE cap
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PP
PP chamber
PE cap
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Includes ready to use chambers
with pre-installed membrane
and 2 sample tubes (1.5 ml) per chamber
(centrifuge recommended, sold separately)
Includes chambers with pre-installed
membrane, flotation ring, cap and
2 sample tubes (1.5 ml) per chamber;
(stirrer plate and stir bar sold separately)
Includes chamber, 2 locking rings and 2
membranes; (stirrer plate and stir bar
sold separately)
Includes 2 plate with pre-installed
membrane and 12 8-cap strips
(stirrer plate and stir bar sold separately)
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PTFE=Teflon™; PP=Polypropylene; PE= Polyethylene; PC=Polycarbonate
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
4
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
5
sample preparation
Guide to Sample Preparation Products
EQUILIBRIUM DIALYSIS PRODUCTS for binding studies, protein-protein interactions and protein-drug binding assays
Sample
Volumes (µl)
Number of Stirring Materials Membranes Specials
Membranes Device
MWCO (Da) Accepted
min
max
Multi-Equilibrium DIALYZER™
Up to 20 parallel equilibrium assays
188
750
1,500
3,750
250
1,000
2,000
5,000
Micro-Equilibrium DIALYZER™
A re-usable equillibrium dialysis 2or 3-chamber system for studying
protein binding assays
19
38
75
188
375
25
50
100
250
500
1
No
PTFE
Body
Fast Micro Equilibrium DIALYZER™
A re-usable equillibrium dialysis
2-chamber system for protein
binding studies (2-5 times faster than
Micro-Equilibrium DIALYZER)
19
38
75
188
375
25
50
100
250
500
1
No
PTFE
Body
Dispo Equilibrium DIALYZER™
Single sample, single use product
for interaction studies
25
75
1
96-Well Equilibrium DIALYZER™
Single use product for 96
simultaneous assays
50
300
Ordering
Information
REUSABLE EQUILIBRIUM DIALYSIS PRODUCTS
1/Chamber Built-in
Yes
Includes Drive Unit,
1 package of 200
membranes (10,000
MWCO), 20
emptying stoppers,
4 carriers, 120 plugs,
24 spacers, 1 filling
clamp and stand; 20
cells; (Tank for
temperature control
available as an
option).
Regenerated
Yes
Cellulose
1,000 to
50,000,
Cellulose Acetate
100 to 300,000
Polycarbonate
0.01 µm to 0.60 µm
Includes 1 chamber,
1 link and 2 solid
caps in a package
for 2-chamber
system; (membranes
sold separately).
Additional links for
3-chamber system
available. Other
membrane
sizes available.
PTFE Regenerated
chamber Cellulose
Delrin™ 5,000 or
plugs
10,000
Regenerated
Yes
Cellulose
1,000 or 5,000,
Cellulose Acetate
100 to 300,000
Polycarbonate
0.01 µm to
0.60 µm
Includes 1 chamber,
1 link and 2 solid
caps per package;
(membranes sold
separately)
EQUILIBRIUM DIALYSIS PRODUCTS
PTFE=Teflon™; PP=Polypropylene; PE= Polyethylene; PC=Polycarbonate
PP Body
PE Caps
5,000 or
10,000
Yes
Includes 2 chambers
with membrane, 2
caps (one black, one
white), 2 sample
tubes (0.65 ml) and 2
special pipette tips.
Other membrane
sizes available
1/Chamber Rotator PP Body
PE Caps
5,000 or
10,000
Yes
Includes plates w/
membranes, 12 top
strips and 12 bottom
strips; (rotator sold
separately).
Harvard Apparatus • phone 508.893.8999 • toll free U.S. 800.272.2775 • fax 508.429.5732 • www.harvardapparatus.com
6