Sample protocol for fish morphological traits in MEDITS surveys

Anik Brind'Amour, Bastien Mérigot and Angélique Jadaud
April 25th 2011
Sample protocol for fish morphological traits in MEDITS
surveys
Effective classification of fish species in functional groups depends on the quality of the
biological measurements. Following this simple sampling protocol will ensure the building
of an harmonized database of fish morphological traits across the Mediterranean areas.
Species list
A list of 96 species was established (Annexe 1). It includes all the reference species in
the MEDITS survey, several species from the catch data and some species used in a
recent paper from Gaertner et al. (2010). That list covers a large spectrum of species
thereby allowing the development and comparison of community models with sufficient
data.
Measurements of fish traits should be made on 10 individuals per species. All the
individuals must be (i.e. mature).
Morphological traits of species
Species traits were chosen to minimize the additional work on board. We identified traits
that will be measured from pictures taken on board and traits that have to be assigned
and measured on board (Appendix 2).
Steps to be followed for the measurements of
each individual:
1) Take a picture and identify each picture
2) Measure the oral gap
All the information needed from the pictures and the traits measured on board should be
given in a table that looks like the one in Appendix 3.
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Anik Brind'Amour, Bastien Mérigot and Angélique Jadaud
April 25th 2011
1) Protocol for pictures on board (Figure 1)
•
Take a picture of each fish individual (10 individuals by species). Pictures should
be clear and well identified. Each picture should be identified as follow:
• Species code (see Appendix 1)
• Individual n°
• Station n°
• Country (eg. ESP, FRA, ITA, GRC , ...)
• Vessel (eg. AND, BIM, …)
•
A scale (e.g. a ruler) should be put beside the fish in order to calibrate later the
measurements (see Figure 1).
•
For sharks, due to their large size, 2 pictures should be taken for each individual.
Please ensure that these pictures are taken in a way (ie. in continuity) that they
could be assembled later into a single picture with a software, and that a scale (eg.
a ruler) is present on each picture.
•
All pictures should be sent by email to [email protected]. All trait
measurements will be made by Anik Brind'Amour and Bastien Mérigot. If the picture
file is too large, the file can be burned on a cd and send to Bastien Mérigot1.
•
Data (trait values extracted from pictures and measured on board) will be kept
inside the MEDITS group and will not be used for publication purpose without the
agreement of MEDITS colleagues who provided the initial information (picture and
measurements on board). Co-authorship will be proposed for all publications.
Species code
Individual n°
Station n°
Country
AMEINEB 01 565 ITA
Figure 1
Vessel
BIM
Example of a "clear and good" picture with all the identifications needed. (Picture J.-M.
Chamberlan).
1
Centre de Recherche Halieutique Méditerranéenne et Tropicale
UMR 212 EME - Ecosystèmes Marins Exploités (IFREMER-IRD-UMII)
Avenue Jean Monnet - BP 171 - 34203 Sète Cedex - France.
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Anik Brind'Amour, Bastien Mérigot and Angélique Jadaud
April 25th 2011
2) Protocol for fish measurements on board vessels (Figure 2)
There is 1 trait that must be measured as follow on board:
1) Oral gap
The oral gap (Og) is the measure in cm of the open mouth once the mouth is extended to
its maximum opening. Oral gap can be measured with either using a caliper or a ruler.
Og
©sbg.ac.at
Figure 2
Example of the measurement of the oral gap (Og) for Scomber scombrus.
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Anik Brind'Amour, Bastien Mérigot and Angélique Jadaud
April 25th 2011
Appendix 1.
measured.
List of Mediterranean fish species to be
Species
Aphia minuta
Arnoglossus imperialis
Arnoglossus laterna
Arnoglossus rueppelii
Arnoglossus thori
Argentina sp.
Aspitrigla cuculus
Blennius ocellaris
Boops boops
Bothus podas
Buglossidium luteum
Callionymus sp.
Capros aper
Carapus acus
Centracanthus cirrus
Cepola macrophthalma
Chelidonichthys lastoviza
Chelidonichthys lucernus
Chelidonichthys obscurus
Citharus linguatula
Conger conger
Dasyatis pastinaca
Deltentosteus quadrimaculatus
Dicentrarchus labrax
Diplodus annularis
Diplodus vulgaris
Echelus myrus
Engraulis encrasicolus
Eutrigla gurnardus
Gobius sp.
Gadiculus argenteus
Gaidropsarus sp.
Galeus melastomus
Helicolenus dactylopterus
Lepidopus caudatus
Lepidorhombus boscii
Lepidorhombus whiffiagonis
Lepidotrigla cavillone
Lepidotrigla dieuzeidei
Lesueurigobius sp.
Species code
APHIMIN
ARNOIMP
ARNOLAT
ARNORUE
ARNOTHO
ARGESPP
ASPICUC
BLENOCE
BOOPBOO
BOTHPOD
BUGLLUT
CALMSP
CAPOAPE
CARAACU
CECACIR
CEPOMAC
CHELLAS
CHELLUC
CHELOBS
CITHMAC
CONGCON
DASIPAS
DELTQUA
DICELAB
DIPLANN
DIPLVUL
ECHEMIR
ENGRENC
EUTRGUR
GOBISPP
GADIARG
GAIDSPP
GALUMEL
HELIDAC
LEPICAU
LEPMBOS
LEPMWHS
LEPTCAV
LEPTDIE
LESUSPP
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Anik Brind'Amour, Bastien Mérigot and Angélique Jadaud
April 25th 2011
Lithognathus mormyrus
Lophius budegassa
Lophius piscatorius
Macroramphosus scolopax
Merlangius merlangus
Merluccius merluccius
Microchirus variegatus
Micromesistius poutassou
Monochirus hispidus
Mullus barbatus
Mullus surmuletus
Mustelus mustelus
Oblada melanura
Ophidion barbatum
Ophichthus rufus
Pagellus acarne
Pagellus bogaraveo
Pagellus erythrinus
Pagrus pagrus
Peristedion cataphractum
Phycis blennoides
Psetta maxima
Raja asterias
Raja clavata
Sarpa salpa
Scomber japonicus
Scomber scombrus
Scophthalmus rhombus
Scorpaena elongata
Scorpaena loppei
Scorpaena notata
Scorpaena porcus
Scorpaena scrofa
Scyliorhinus canicula
Serranus cabrilla
Serranus hepatus
Solea solea
Sparus auratus
Sphyraena sphyraena
Spicara maena
Spicara smaris
Spondyliosoma cantharus
Sprattus sprattus
Squalus acanthias
Symphurus sp.
LITHMOR
LOPHBUD
LOPHPIS
MACOSCO
MERNMER
MERLMER
MICUVAR
MICMPOU
MONOHIS
MULLBAR
MULLSUR
MUSTMUS
OBLAMEL
OPDIBAR
OPHCRUF
PAGEACA
PAGEBOG
PAGEERY
PAGRPAG
PERICAT
PHYIBLE
PSETMAX
RAJAAST
RAJACLA
SARPSAL
SCOMPNE
SCOMSCO
SCOPRHO
SCORELO
SCORLOP
SCORNOT
SCORPOR
SCORSCR
SCYOCAN
SERACAB
SERAHEP
SOLEVUL
SPARAUR
SPHYSPY
SPICMAE
SPICSMA
SPODCAN
SPRASPR
SQUAACA
SYMPSPP
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Anik Brind'Amour, Bastien Mérigot and Angélique Jadaud
April 25th 2011
Syngnathus acus
Torpedo marmorata
Torpedo nobiliana
Trachinus draco
Trachinus radiatus
Trachurus mediterraneus
Trachurus trachurus
Trigla lyra
Trigloporus lastoviza
Trisopterus minutus
Uranoscopus scaber
Zeus faber
SYNGACU
TORPMAR
TORPNOB
TRAHDRA
TRAHRAD
TRACMED
TRACTRA
TRIGLYR
TRIPLAS
TRISCAP
URANSCA
ZEUSFAB
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Anik Brind'Amour, Bastien Mérigot and Angélique Jadaud
April 25th 2011
Appendix 2. List of species traits.
Table 1
Description of the species traits that will be measured from pictures after
surveys by Anik and Bastien.
Traits measured from
pictures taken on board
Body depth
Caudal length
Eye diameter
Barbel
Pigmentation
Body shape
Code
Description
Body
Caudal
Deye
Bar
Pig
Sh
Ratio of standard length to body depth
Ratio caudal fin length to caudal fin depth
Diameter of the eye standardized by head length
The presence of barbel near the mouth
Pigmentation on the fish body
Fish body shape
Mouth orientation
Mo
Mouth orientation
Trait measured and
assigned on board
Oral gap
Og
Oral gap
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Anik Brind'Amour, Bastien Mérigot and Angélique Jadaud
April 25th 2011
Appendix 3. Example of the table to be filed on board. The table contains all the
information relative to the picture and the measurements made on board.
Station n°
Species
Individual
Picture reference n°
Oral gap (cm)
565
SCOMSCO
1
SCOMSCO 01 565 ITA BIM
4.5
565
SCOMSCO
2
SCOMSCO 02 565 ITA BIM
4.3
565
SCOMSCO
3
SCOMSCO 03 565 ITA BIM
4.4
565
SCOMSCO
4
SCOMSCO 04 565 ITA BIM
4.5
565
SCOMSCO
5
SCOMSCO 05 565 ITA BIM
4.6
8