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DGT-CO2BX, Digital CO2 Controller
Manual
Ver.02/2008
1 Preface
DGT-CO2BX is a Digital Control Unit that generates a CO2-Air mixture with an adjustable
CO2 concentration in the range 0-20%, with an accuracy of ± 5% of CO2 concentration, for
instance, if CO2 set point is 5%, accuracy is ± 0.25%. DGT-CO2BX can be used as a
stand-alone device as well as in combination with any kind of microscope incubator. The
air flow is regulated by a floating ball air flow meter in the range 0.2 – 0.8 Nliters/min. A
CO2 infra red sensor continuously measures CO2 concentration in the mixed gas stream
and a PID closed loop controller gives feedback to a fine valve regulating CO2 flow.
Measured value of CO2 concentration is displayed in real time in green (DGT-CO2BXPLUS displays also set point value). An alarm can be set on CO2 concentration, so that, if
CO2 concentration goes below such value, display colour turns red and optionally a buzzer
turns on. In the model DGT-CO2BX-PLUS the serial RS-232 interface and the CO2 Control
Software allow the user to control the unit with a personal computer and to acquire and
store concentration data in computer memory.
The DGT-CO2BX, CO2 Digital Controller, together with the Okolab Microscope Stage
Incubation Systems is designed to maintain all the required environmental conditions for
cell cultures right on the microscope stage, thus allowing prolonged observations of cell
events.
2
2 Index
1 PREFACE ...............................................................................................................2
2 INDEX ....................................................................................................................3
3 SAFETY NOTES ........................................................................................................4
4 EQUIPMENT ............................................................................................................5
4.2 E Q U I P M E N T S U P P L I E D .................................................................................................................................................5
4.2.1 Hardware details.............................................................................................5
4.2.2 Tubing details................................................................................................5
4.2.3 Cables details................................................................................................5
4.2.4 Accessories...................................................................................................5
4.3 E Q U I P M E N T N O T S U P P L I E D ........................................................................................................................................6
4.4 D IGI T A L C O 2 C O N T R O L L E R S E T T I N G ...........................................................................................................................7
4.4.1 Front and Rear of the DGT-CO2BX- PLUS Overview...................................................7
5 CONNECTIONS ........................................................................................................8
5.2 S E T T H E G A S LI N E S ....................................................................................................................................................8
5.3 T UB E S C O N N E C T I O N ....................................................................................................................................................9
5.3.1 Connect DGT-CO2BX with Humidifying Module or Specimen Chamber.............................9
5.3.2 Connect Gas reservoirs with DGT-CO2BX Unit........................................................10
5.3.3 Fix the “Filter holder” to the rear panel of the DGT-CO2BX Unit.....................................10
6 PC CABLES CONNECTIONS .......................................................................................12
7 CHECK YOUR INTERNATIONAL OPTIONS .....................................................................12
8 INSTALL THE SOFTWARE .........................................................................................13
9 CHANGE THE SET POINT..........................................................................................13
10 STARTING THE EXPERIMENT AND GETTING THE BEST OUT OF YOUR INCUBATION SYSTEM .15
1 0.2 O R G A N I Z E T H E E X P E R I M E N T ....................................................................................................................................1 5
1 0.3 A IR F L O W M E T E R R E G U L A T I O N ...............................................................................................................................1 5
11 APPENDIX ............................................................................................................16
1 1 . 2 D G T- C O 2 B X P A R A M E T E R S .................................................................................................................................1 6
11.2.1 Factory parameters.......................................................................................16
12 SUPPORT ............................................................................................................17
1 2.2 W E B C O N F E R E N C E F O R A S S I S T A N C E A N D T R A I N I N G ....................................................................................................1 7
1 2.3 T R O U B L E S H O O T I N G ................................................................................................................................................1 7
1 2.4 T E C H N I C A L S U P P O R T ..............................................................................................................................................1 8
1 2.5 M AI N T E N A N C E .......................................................................................................................................................1 9
12.5.1 External surfaces maintenance.........................................................................19
1 2.6 T E C H N I C A L S P E C I F I C A T I O N S ....................................................................................................................................20
3
3 Safety Notes
Before operating the equipment please read carefully the instructions and the safety notes.
Read carefully also the supplied on request Omega® Process Controller manual. If you
have any questions, please contact Okolab assistance.
 Equipment should only be operated by technically qualified personnel
 Equipment and its internal parts can be damaged by dropping and by shock
 Before starting, assembly the equipment far from your microscope and/or electric
device
 Do not start up the equipment if the supply cable is damaged
 After the use, disassembly the equipment far from your microscope and/or electric
device
 Connect the equipment only to grounded mains power socket
 Prevent throttling and kinking of tubing
 Check gases tubes time to time for possible material usage
 Check the tubes are well fixed so they cannot slip off
 Use distilled treated water for gas stream humidification. For further informations
please contact us before to use it.
 The equipment must only be used as intended and as described in this Manual. This
includes operation by suitably instructed qualified personnel
 This device is not designed for use under medical conditions
 Do not use the flow meters as valves. Once regulated the flow rate (at P=1bar) use the
flow meters only for fine regulations. Install safety valves on gas lines.
 We reserve the right to make technical variations.
 Change the Filters periodically as following described.
International caution symbol marks this device. It is important to read the
Safety Notes before installing, using and commissioning this device, as
the notes contain important information relating to safety and EMC.
IN NO EVENT SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL OR
CONSEQUENTIAL DAMAGES OF ANY NATURE, OR LOSSES OR EXPENSES
RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE OF ANY PRODUCT.
CAUTION- Risk of electric shock! Do not open the ‘Box’. To prevent the risk of electric
shock, do not remove cover or back. No user serviceable parts inside.
4
4 Equipment
4.2 Equipment supplied
The DGT-CO2BX (-PLUS), CO2 Digital Controller, is comprised of the following
components:
Hardware:
#1 device, DGT-CO2BX (-PLUS-S)
Software (Optional):
#1 CD-ROM with DGT-CO2BX-PLUS-S Software
Plastic connection tubings kit + accessories
Electric Cables
4.2.1 Hardware details
1) DGT-CO2BX Version, Digital CO2 Controller. CO2 can be regulated in the range 0-20%
with great accuracy. Measured CO2 value is displayed in real time.
2) DGT-CO2BX-PLUS Version, Digital CO2 Controller Plus. Same as DGT-CO2BX +
RS232 serial port to control and read CO2 levels via software. Measured CO2 and set
point values are displayed in real time.
3) External Air Pump (Upon request).
4.2.2 Tubing details
4) #2 (or more depending on model) white rigid tubes of 6mm OD, 3 m long.
5) #1 tube composed by 1 meter of blue rigid tube of 6mm OD at each end, 50cm of
silicon tube of 4mm I.D. with a filter, degree of filtration 0.2 micron.
6) Accessory to connect together the blue rigid tube and the silicon tube of item 5).
4.2.3 Cables details
7) #1 power supply cable.
8) #1 RS232 communication cable (Optional) included in the DGT-CO2BX-PLUS system.
9) #1 RS232/USB converter cables and its installation CD (Optional) included in the DGTCO2BX-PLUS system.
4.2.4 Accessories
10)CD with control software (-PLUS-S).
11)Fixing-tubes kit (to fix tubes on the microscope table).
12)Pressure Gauge for CO2 with regulator, degree of filtration 20 μm and condensate
drain (supplied).
13)Pressure Gauge for pressurised Air line with regulator, degree of filtration 20 μm and
condensate drain (not supplied, upon request).
14)Gas filter, degree of filtration 0.2 micron with #2 silicon tubing 10cm long (supplied).
15)Gas filter holder.
5
4.3 Equipment NOT supplied
 Personal Computer (OS: Windows 2000 /Xp) with #2 RS232 Serial Communication
Ports or #1 USB Port.
 CO2 reservoir.
 Air reservoir (It can be substituted by a compressed air line or External Air Pump)
 Pressure Gauge for Air with regulator, degree of filtration 20μm and condensate drain
(provided by Okolab on request).
6
4.4 Digital CO2 Controller setting
Depending on you chose of the Okolab Digital CO2 Controller version, the Front and Rear
will be different.
Tip►DGT-CO2BX
and DGT-CO2BX-PLUS versions only differ on the Display
Tip►
Screen and on the presence of the RS232 serial communication port on the Rear.◄
◄
IMPORTANT
This manual is referred to the version DGT-CO2BX-PLUS
4.4.1 Front and Rear of the DGT-CO2BX-PLUS Overview
1. Display (measured and set point value)
2. CO2 fine valve controller
3. Floating ball Air flow meter
4. 0.2 µm filter
5. RS232 or infra red sensor calibration
6. RS232 to PC
7. Air inlet
8. CO2 inlet
9. Mixed gas outlet
10. Power switch with #2 120mA-T Fuses
Figure 1: Front and Rear of the DGT-CO2BX-PLUS Overview.
7
5 Connections
5.2 Set the gas lines

Provide your system of CO2 reservoir with safety valve and pressure gauge with
pressure rigid tubes 6 mm OD exiting (Figure 3.number 1).

Provide your system of Air reservoir (Okolab suggests to use a compressed Air line)
with safety valve and pressure fine gauge with pressure rigid tubes 6 mm OD exiting
(Figure 3.number 4). If a compressed air line is not present in the lab an Air Pump with
filter inside can be used. It is not included in the system, upon request (Figure
3.number 5).

Provide your system of O2 instead of Air (optional configuration) reservoir, or
compressed line, with safety valve and pressure gauge with pressure rigid tubes 6 mm
OD exiting (Figure 3.number 4).

Mount the Pressure Gauge for CO2 with regulator, degree of filtration 20μm and
condensate drain (supplied) on the wall vertically-(Figure 3.number 2).

Be sure that Air stream is FILTRATED and with a STABLE Pressure Gauge otherwise
mount another Pressure Gauge for air with regulator, degree of filtration 20μm and
condensate drain (non supplied, if you need ask to Okolab for a prompt shipping)(Figure 3.number 6). If there is no pressurised air line in the lab an Air Pump with filter
inside can be used.

Follow the arrow on the Pressure Gauge for the correct gas In-Out. (see Figure 2)
Figure 2: Pressure Gauge: Correct Gas Input – Output.
8
1. CO2 reservoir with Pressure Fine Gauge
2. Pressure Gauge for CO2 with regulator, 20 micron filter inside
3. White rigid tube with 6mm O.D., 3m long
4. Compressed air line with its Pressure Gauge
5. Air pump with filter inside, upon request instead of pressurised air line
6. Pressure Gauge for Air with regulator, 20 micron filter inside
7. White rigid tube with 6mm O.D., 3m long
8. Blue rigid tube with 6mm O.D., 3m long
Figure 3: Gas lines scheme from reservoirs to Control Unit
5.3 Tubes connection
5.3.1 Connect DGT-CO2BX with Humidifying Module or Specimen Chamber
Connect the Blue marked tubing “#1 tube composed by 1 meter of blue rigid tube of 6mm
OD at each end, 50cm of silicon tube of 4mm I.D. with a filter, degree of filtration 0.2
micron.” listed before to the “push-in” connector labelled as “Air + CO2 out” on the rear
panel of the DGT-CO2BX and to the preheating module or to the humidifying module or
directly to the chamber, depending on the incubating system model.
Figure 4: Zoom of the blue tube and silicon
tube connection.
Tip► Look at the instructions written on the labels attached on the transparent
plastic bags.◄
◄
Tip► In order to avoid gas leak and correctly insert the tube, please, strongly push
the tube into the Swift-Fit connector.◄
◄
9
Tip► Connect the 4mm I.D. tube to the humidifying Module or directly to the
Incubating Chamber if the preheating module is not present.◄
◄
Tip► Look at the colour of the tubes and of the adhesive stripes attached on these
devices.◄
◄
Tip► This tubing moves the Air and CO2 gas mixture to the Specimen Chamber.◄
◄
5.3.2 Connect Gas reservoirs with DGT-CO2BX Unit

White rigid tubes (4):
Connect the controlled (filtered and at P=1atm) flows of air and CO2 (O2 optional
configuration) from Pressure Gauges provided by to the DGT-CO2BX. Insert the tubes into
the Swift-Fit connectors (Air IN and CO2 IN on the rear of the Unit, see Figure 3.
Tip► If you want to disconnect the tube, please, pull the tube while pushing the
black ring on the Swift-Fit connector (Figure 5).◄
◄
Tip► In order to avoid gas leak and correctly insert the tube, please, strongly push
the tube into the Swift-Fit connector.◄
◄
5.3.3 Fix the “Filter holder” to the rear panel of the DGT-CO2BX Unit
Tip► The DGT-CO2BX Unit is equipped with two additional Hydrophobic PTFE
filters with degree of filtration 0.2 microns for the protection against bacteria and
condensations.
One of them has to be mounted on the rear panel of the control box. It filters the Air and
CO2 flows coming from their tanks before entering into the carbon dioxide sensing cell. It
prevents sample contamination from bacteria and infra red CO2 sensor from damages
caused by dirty gas streams. It is connected with two 4 I.D. mm silicon tubes with the
“Filter In” and “Filter Out” connectors on the Rear of the DGT-CO2BX. It is fixed by a
support, called "Filter Holder" to the Control Unit. This "Filter Holder" is fixed to the rear of
the control box with two screws. Refer to Figure 6 for the instructions about how to mount
this “Filter holder” to the rear panel of the control box.
Another Hydrophobic PTFE filter is present along the Blue marked tubing “#1 tube
composed by 1 meter of blue rigid tube of 6mm OD at each end, 50cm of silicon tube of
4mm I.D. with a filter, degree of filtration 0.2 micron”. This filter avoid contamination
coming from the Humidifying module to the sensing cell, done by a small back pressure,
that may occur during the CO2 or Air pressurized lines closing.
Tip►Replace
the gas filters each 2 months of continuous use (Figure 1, number 4;
Tip►
Figure 6). Okolab has these filters at stock. Contact Okolab to purchase them.
Do not remove the filters, absolutely. This may cause damages to the sensing cell
and sample contaminations.
Avoid narrowing of the tubes and formation of air bubbles inside them. Please,
periodically check fluids are correctly flowing and tubes are properly connected.
10
Figure 5: Avoid gas leak: strongly push the tube into the Swift-Fit connector
Figure 6: Gas Filter
1. Filter holder. Metal sheet with two clamps.
2. #2 M3x8 screws.
3. #2 Silicon tubes 8-10 cm long
4. PTFE Hydrophobic Filter, degree of filtration 0.2 microns.
!. Connect the Inlet of the the Filter to the “Filter In” connector on
the rear panel of the control box. The inlet of the filter is labelled
on the transparent plastic filter housing.
11
6 PC cables connections
Connect the DGTCO2BX-PLUS to your PC using the RS232 Serial Cable supplied.
II. Use the RS232/USB converter cable if your PC does not have RS232 serial
communication port available. Please, install the driver on your PC using RS232/USB
converter cable installation CD and re-start your PC.
I.
7 Check your International options
IMPORTANT
For a proper work of the “Okolab Read Temperature” software, you MUST select the point
as decimal separator in the International options of your PC, following this path:

Start > Control Panel > Regional and Language Options > Regional Options >
Customize

Click Start

Select Control Panel



Select Regional and Language Options
.
.
Click Customize
Select
or
write
in the Control Panel.
in the Regional Options window.
the
‘point’
as
decimal
symbol
in
the
text
box
.

Select or write the ‘space’ or ‘comma’ as digit grouping symbol in the text box
.

Click Apply
than OK
2 times.
12
Figure 7: Regional and Language Options Panel
8 Install the Software



Insert the Installation CD-rom on your PC.

Re-start the Pc.
Launch the setup.exe and follow the instructions on the screen.
Once you have completed the installation, the program will appear on your PC
Programs Menu.
9 Change the Set Point
The electronic controller has two different Set Points. Te Set Point 1 (SP1) represents the
controlled CO2 value and the Set Point 2 (SP2) represents the desired steady state value
for Alarm functions. The Unit normally displays the current CO2 concentration and the Set
13
Point 1. The SP1 is 5.00%, as default. SP2 has to be equal to SP1. If you wish to change
manually the two Set Point values, please read the following instructions:
Figure 8. Electronic controller display.
IMPORTANT
Do NOT change others parameters already adjusted to optimize the performances of the
Unit.



Press

Holding
&
buttons down for approximately 3 seconds will speed up the rate at
which the Setpoint value increments or decrements.

Press
value.

Press
, if necessary until SP1 prompt appears.
1) Press
Press
2) Display shows previous value of “Setpoint 1”.
Press
&
respectively.
3) Press
&
and
4) Continue to use
to increase or decrease Setpoint 1
and
to enter your 4-digit Setpoint 1
5) Display shows STRD stored message momentarily and then
advances to SP2 only, if a change was made, otherwise press a to advance to SP2
Setpoint 2 Menu. Do the same for SP2.
The software supplied by Okolab allows to change the Set point during an experiment and
to change the alarm configurations. It reads the Carbon Dioxide percentage in time and
stores the data in the computer memory. Ask to Okolab for further informations.
14
10 Starting the experiment and Getting the best out of
your incubation system
10.2 Organize the experiment

Read carefully the manual of the Incubating System you are using to control the
specimen temperature. If the temperature controller has been supplied by Okolab, ask
to us how to get the best from the incubating system equipped with the DGT-CO2BX.
IMPORTANT
Overloading the Humidifying Module could induce condensation inside the tubes, on
the windows surface of the incubating chamber and on the surfaces of plastic
supports, not depended on the DGT-CO2BX.
Avoid gases back flow.

Check that all tubes are properly connected and prevent narrowing, throttling, kinking
of tubing, and formation of air bubbles.

Check the tubes are well fixed so they cannot slip off using the kit supplied.

Switch on the Temperature Controller.
10.3 Air Flow Meter regulation
IMPORTANT
Do not use the flow meters as closing-opening valves. Once regulated the flow rate (at
P=1bar, see Figure 3, use the flow meters carefully only for fine regulations.
Open the Air gas valves slowly, regulate the pressure at 1 bar. Otherwise turn on the Air
Pump with filter inside. Regulate the floating ball of the Air Flow Meter within the range 0.3
– 0.8 Litres/min.
Air flow rate values and
CO2 consumption
[Nl/min]
Air
CO2
Air
CO2
Total
95.00
5.00
0.30
0.016
0.316
95.00
5.00
0.4
0.021
0.421
95.00
5.00
0.5
0.026
0.526
Table 1. CO2 consumption for different air flow rates. [Nl = Normal litre]
Target Gas Percentage
[%]
Tip ► If you need, ask Okolab for personalized Gas Percentage Tables. ◄
15
•
'Target Gas Percentage’ indicates the stream gas percentage that the operator wants during the
experiment.
•
‘Air flow rate values and CO2 consumption’, in Normal litre per minute [NL/min], are the values of
air flow rate set on the graduated scale and the corresponding CO2 consumption.
•
'Total’ indicates the measured total gas stream flow rate.

Regulate the Floating ball Air Flow Meter at the desired value within the range 0.3-0.8
NL/min.


Open the pressure gauge of the CO2 reservoir and set it at 1.5-2 bar.


Turn on the DGT-CO2BX.


You can start the experiment and carry on the specimen into the incubating chamber.

System has been calibrated at the factory. It is maintenance free. Sensor is stable up
to five years of work.

We suggest to calibrate the Unit each two years of continuous use. Contact the Okolab
Assistance to do the calibration.
Regulate the Pressure Gauge for CO2 with regulator, 20 micron filter inside, supplied
by Okolab at 0.9-1 bar. Be sure that no pressure drop happens. Use this pressure
regulator as closing valve when you finish the experiment too.
After few second the CO2 percentage is displayed on the Unit. After half an hour the
CO2 reach the steady state value (5.00% as default).
For further informations and especially use of the Unit, please feel free to contact the
Okolab people.
11 Appendix
11.2 DGT-CO2BX parameters
The PID closed loop controller reads the CO2 level within the gas stream and gives
feedback to a fine valve regulating CO2 flow with an accuracy of ± 5% of CO2 percentage.
The PID parameters were stored to obtain the maximum performances from the system in
terms of stability and fast recovery, after a gas flow changing due to quickly changed of
pressures. The controller needs half an hour to reach the steady state after turning on the
unit.
Avoid modifying the controller factory parameters. Some parameters alterations could
make the system bad-functioning or could induce the system failure. Please, contact
Okolab assistance in case, some control parameters, in PID display, were accidentally
garbled.
11.2.1 Factory parameters





Setpoint1:
5.00%;
Setpoint1:
5.00%;
Anti integral:
Disable;
Proportional:
50.0;
Reset:
150;
16






Rate:
3.0;
Damping:
4;
Thermocouple type:
k;
Color Display:
Green;
Temperature Unit: ”
°C”
Filter Value:
16.
12 Support
12.2 Web conference for assistance and training
In case you requested on line support and/or web conference assistance for system
assembling and experiment support, you received a web cam. Please follow these rules to
receive a good service:

Webcam installation (instruction and Cdrom included)


Last MSN Messenger ® installed (www.msn.com)

Set the audio and video and test them using MSN Messenger.

If you have problem with MSN Messenger you can choose Skype ® software
(www.skype.com).

Contact our technical support ([email protected]) by e-mail to take an appointment
for the web assistance.
Register yourself on www.msn.com to have an account (MSN passport)
12.3 Troubleshooting
Incorrect operations are often mistaken for trouble and malfunctions. If you think that there
is something wrong with a component, check the points below. Sometime the trouble may
lie in another component. Investigate the others components, electrical appliance and
pneumatic system being used. If the trouble cannot be rectified even after exercising the
checks listed below, ask Okolab service.
Symptom
No power supplied to Unit
Probable cause
Power plug is disconnected
from outlet
Blown fuse
Pressure drop
No CO2 and/or Air flow rate stability
Air Flow too low
17
Remedy
Insert plug securely into outlet
Replace the fuse, 120mA-Time Lag
Fuses
Check reservoir and/or compressed
line
Change the PTFE filter with degree of
filtration 0.2 micron
Check the air flow with the Floating
ball flow meter.
Increase air flow.
Symptom
Probable cause
No CO2 and/or Air flow streams
RS 232 cable not plugged
in
USB-RS232 converter
cable not plugged in
USB-RS232 converter
cable driver not installed on
this USB port
Serial Port communication
numbers not inserted
Regulators closed
Fast sample drying
Humidifying Module empty
No PC - Unit communication
No CO2 read
The Okolab Software does not work
properly
The Okolab Software does not read
the correct gas percentage displayed
on the Unit
The Unit is off
The PTFE filter with degree
of filtration 0.2 micron is
dirty
The selected decimal
separator is not the point
Probable PC compatibility
problem
Error 1 at open file
An input parameter is invalid.
Saving in an inexistent
Directory
Directory Path not
accessible
Anti-Virus or Firewall make
invalid an input parameter
Gas leak on the roar of the control
box
Not properly inserted tubes
I check the previous troubleshooting
but the system does NOT reach a
stable temperature
Water Bath does not work
properly
The Temperature Meter
does not work properly
Remedy
Insert plug securely into outlet
Use CD rom to install the driver on
this USB port
Click on the Settings button of Okolab
Software and follow the instructions
Open regulators
Fill it with distilled water until 50 mm
below the top of glass column.
Switch on the Control Unit
Change the PTFE filter with degree of
filtration 0.2 micron
Select the point as decimal separator
in the International options of your PC
Contact Okolab to receive assistance
Verify the Directory Path
Change your account type. Ask to
computer Administrator
Disable Anti-Virus or Firewall activities
on Okolab Software
Strongly push the tube into the SwiftFit connector
Contact Okolab to receive assistance
Contact Okolab to receive assistance
12.4 Technical support.
Please, do not hesitate to contact Okolab should you need any further commercial
information or technical support.
Please, check Okolab web site www.oko-lab.com for news, events, new products and
general FAQ.
For COMMERCIAL SUPPORT:
[email protected]
Phone
+39 081 806 2624
Fax:
+39 081 876 4410
Mobile:
+39 338 981 1804
For TECHNICAL SUPPORT:
[email protected]
Phone
+39 081 806 3470
Fax:
+39 081 876 4410
Mobile:
+39 328 138 7415
For SOFTWARE SUPPORT:
[email protected]
18
Phone
+39 081 806 3470
Fax:
+39 081 876 4410
Mobile:
+39 328 972 1146
Published by Okolab S.r.l.,Via G. di Prisco, 152, 80040 Ottaviano (NA), ITALY
12.5 Maintenance
12.5.1 External surfaces maintenance

Use a polishing cloth or dry cloth to wipe off dust and dirt.

Never use thinners, benzene, solvents on or near the devices, since these could
corrode their surfaces.

Change the silicon tubes after one year of continuous use.
19

12.6 Technical Specifications
Specification Table
Product code
Sensing technology
CO2 Set Point value range
Accuracy
Repeatability of CO2 concentration
Air Flow rate
Filtering device
Digital interface (RS232)
Communication Software (-S)
Dimensions
Weight
Voltage/Frequency
Compatibility


Unit:
DGT-CO2BX (-PLUS)
Non dispersive infra red (NDIR) dual wave length
detector
0 – 20%
± 5% of CO2 concentration , i.e. ± 0.25% if CO 2 is
5%
Below 0.1%
From 0.2 Nl/min to 0.8 Nl/min
Reusable PTFE membrane with 0.2 micron pores
RS232 to PC
Stand alone version and Plug-in version for all
Okolab Software
206x178x310 mm
3.5 kg
110-230 V / 50-60 Hz
Any microscope incubator
Input 90-240Vac ± 10%; 50-60 Hz, 6 W; 2 Fuse Time-Lag 125mA.
Air Flow Meters:
Flow Rate range 0.1-1.0 Nl/min, 5%full scale error; Pmax 6.0 bar, Operating pressure 1.0 bar at 20°C.
20
Figures
FIGURE 1: FRONT AND REAR OF THE DGT-CO2BX- PLUS OVERVIEW.......................................7
FIGURE 2: PRESSURE GAUGE: CORRECT GAS INPUT – OUTPUT............................................8
FIGURE 3: GAS LINES SCHEME FROM RESERVOIRS TO CONTROL UNIT..................................9
FIGURE 4: ZOOM OF THE BLUE TUBE AND SILICON TUBE CONNECTION..................................9
FIGURE 5: AVOID GAS LEAK: STRONGLY PUSH THE TUBE INTO THE SWIFT-FIT CONNECTOR . . . .11
FIGURE 6: GAS FILTER ................................................................................................11
FIGURE 7: REGIONAL AND LANGUAGE OPTIONS PANEL .....................................................13
FIGURE 8. ELECTRONIC CONTROLLER DISPLAY...............................................................14
Tables
TABLE 1. CO2 CONSUMPTION FOR DIFFERENT AIR FLOW RATES. [NL = NORMAL LITRE]..........15
WARRANTY
Okolab S.r.l. warrants its products to be free of defects in materials and workmanship for a
period of one year starting from invoice date. If the unit malfunctions, it must be returned to
the factory for evaluation. If the equipment has to be returned to the factory, please ensure
that is carefully and properly packed. Okolab S.r.l. accepts no responsibility for damage
due to unsatisfactory packing. Upon examination of Okolab S.r.l., if the unit is found to be
defective, it will be repaired or replaced at no charge. This warranty does not apply to
defects resulting from any actions of the purchaser. Components which wear are not
warranted. Okolab S.r.l. neither assumes responsibility for any omissions or errors nor
assumes liability for any damages that result from the use of its products in accordance
with information provided by Okolab S.r.l.
Okolab S.r.l. warrants only the parts manufactured by it will as specified and free of
defects. Okolab S.r.l. makes no other warranties or representations of any kind
whatsoever, express or implied, except that of title, and all implied warranties including
any warranty of merchantability and fitness for a particular purpose are hereby disclaimed.
LIMITATION OF LIABILITY: the total liability of Okolab S.r.l. shall not exceed the purchase
price of the component upon which liability is based. In NO event shall Okolab S.r.l. be
liable for consequential, incidental or special damages.