Supplementary Figures Supplementary Figure S1
Nordsjællands Hospital Method comparison of CTC detection with ® ® CytoTrack and CellSearch Ann-Britt Nygaard1, Peer Horn2, Anastasiya Haugaard1, Sarah Nejlund1, Jörg Klingelhöfer3, Ivan Brandslund2, György Sölétormos1, Thore Hillig1 1Department of Clinical Biochemistry, Nordsjællands Hospital, Hillerød, Denmark 2Department of Clinical Biochemistry, Lillebælt Hospital, Vejle, Denmark 3Department of Neuroscience and Pharmacology, Panum Institute, University of Copenhagen, Denmark Objective: Assess performance of CytoTrack® compared to CellSearch® CTC analysis. Method MCF-7 tumor cells were spiked into donor blood samples or directly onto microscope glass slides using ® ® FACSAria cell sorter. Recoveries of MCF-7 cells were analyzed with CytoTrack or CellSearch methods. Microscopy (n=3) recovery Spiking efficiency CK MCF-7 tumor cells culturing 150 MCF-7 CellSearch ® DAPI CD45 DAPI/CK recovery (n=5) CytoTrack ® recovery (n=5) Results ® CytoTrack ® CellSearch Recoveries of the MCF-7 cells were determined by the or methods and given as either single MCF-7 cells or events. Results are based on five replicate tests. Error bars represent 95% CI. No significant difference in methods of CTC recovery (p>0.05 ) was observed. MCF-7 Expected Tumor cells Observed MCF-7 cells count Recovery Average SD 95 % CI Average SD 95 % CI % CV CellSearch® 120 102 3 99-105 85 3 83-87 3 CytoTrack® 120 105 13 94-116 87 11 78-97 12 MCF-7 Observed MCF-7 event count Expected Tumor cells Average SD 95 % CI Average Recovery SD 95 % CI % CV CellSearch® 120 93 4 89-96 77 3 74-80 5 CytoTrack® 120 82 13 71-93 68 11 59-78 16 ® ® Conclusion Efficiencies of MCF-7 cells/events recoveries with CytoTrack® and CellSearch® methods were comparable, with recoveries of 87% and 85% respectively. Acknowledgements The study was performed in collaboration with CytoTrack® Aps and Herlev Hospital. The CytoTrack® scanner was funded by Toyota-Fonden, Denmark. The study was funded by a research grant from Nordsjællands Hospital. Thore Hillig, e-mail: [email protected]
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