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Annales de la Faculté des Sciences et Sciences de l'Ingénieur
Vol. 1 N° 3/2009
αΩϧϬϣϟ΍ϡϭϠϋϭϡϭϠόϟ΍ΔϳϠϛΕΎϳϟϭΣ
ťŰŗƄƆŶřŪƆƃŒŗƒƍƒšƃŒŘŒťœŰƆƃŒƍ(Propolis)ŧŕƂŶƃŒŘœŮƄŤřŪƆƇƒŕŗƈŧœƀƆŗŪŒŧť
ƑƃƍŕƃŒŨœƌŞƄƃŗŰŧƆƆƃŒœƒŧřƂŕƃŒ
2ŕŻŕŮŕŗŧƏŸŬƈƓŬŕŬƉŗž 1ũŲŦƗŔŧƈţƈũŕſƅŕŗ
žřƆƁũƏŗšŕŗũƈƒŧŰŕƁřŸƈŕŠūŧƊƎƈƅŔƇƏƆŷƏƇƏƆŸƅŔřƔƆƄž(VPRS)řƔƏŔũţŰƅŔŧũŔƏƈƅŔřƔƁũśƏƉƔƈŝśũŗŦƈ1
ũœŔŪŠƅŔžřƆƁũƏ511ŖůřƔŔŧũŻƀƔũų
[email protected]
žřƆƁũƏŗšŕŗũƈƒŧŰŕƁřŸƈŕŠūŧƊƎƈƅŔƇƏƆŷƏƇƏƆŸƅŔřƔƆƄřžŕŠƅŔƌŗŮƏřžŕŠƅŔƀųŕƊƈƅŔƓžřƔœƔŗƅŔřƈŴƊƗŔřƔŕƈţũƔŦƈ2
žũœŔŪŠƅŔžřƆƁũƏ511ŖůřƔŔŧũŻƀƔũų
[email protected] ŚŕŗŕƎśƅƛřŗŗŬƈƅŔŕƔũśƄŗƅŔřƈƏŕƂƈƓžƇŧŦśŬśƓśƅŔřƔƏƔţƅŔŚŔŧŕŲƈƅŔűŸŗƉƔŗřƊũŕƂƈřŬŔũŧƑƅŏ¿ƈŸƅŔŔŨƍ ŽŧƎƔ ŭŤƄƆƃŒ
Ƒ»Ɔŷř»ŬŔũŧƅŔƋŨƍŚƔũŠŌƏžŕƎƊƈřƔƅŕŸžũŝƄƗŔřžũŸƈƏPropolisũŗƄŸƆƅřſƆśŦƈŚŕŰƆŦśŬƈƏƉŕŬƊƙŔŧƊŷƓƅƏŗƅŔŪŕƎŠƅŔ
aureusžEscherichia coliŕ»ƎƈƍŌƉ»ƈŚŕ»ŗŕƎśƅƛŔƋŨ»ƍŖŗ»ŬśƉŌƉ»ƄƈƔƓ
»śƅŔƏŕ»ƔũƔśƄŗƅŔƉ»ƈŘũ»ƔŝƄŵŔƏƊŌ
Proteus spžProvidanciaspžEntérobacteržStaphylococcus
ũ»ŗƄŸƅŔŚŕ»ŰƆŦśŬƈƅřŗŬƊƅŕŗŕƈŌžŕƎƊƈŧƔŧŸƆƅřƈƏŕƂƈŚŧŗŌƓśƅŔƏŕƔũśƄŗƅŔŧŲřƔƏƔţƅŔŚŔŧŕŲƈƅŔƉƈřſƆśŦƈŵŔƏƊŌŚƆƈŸśŬŔ
řƆƈŸśŬƈƅŔřƔƏƔţƅŔŚŔŧŕŲƈƅŔƉƈřƔƆŷŕžũŝƄŌƐŧŗŌƒŨƅŔųƂžƓƅƏƊŕŝƔƈƅŔůƆŦśŬƈƆƅřƈƏŕƂƈŚŧŗōž
řƔƏƔţƅŔŚŔŧŕŲƈƅŔžřŲũƈƈƅŔŕƔũśƄŗƅŔžřƔƅƏŗƅŔŚŕŗŕŰƙŔžũƄŗŸƅŔŗƒšœřŽƆƃŒŘœƆƄƂƃŒ
Comparison between propolis extracts and antibiotics used against urinary
apparatus bacteria
Summary: This work dialed with comparison of the effect of propolis extracts and some antibiotics
that are used to fighting bacteria of the urinary apparatus, to verify witch one is the most active.
ECBU is the mean to confirm the existence of urinary infection that can be cause by many bacteria
such as: Escherichia coli, Enterbacter sp, Porteus sp, Providancia sp, and Staphylococcus aureus.
The propolis is used after extraction to three extracts E1, E2, E3 and E4, the least one is considered
as waste. The used means are: the propolis, bacteria growth environment, and chemical solvents.
The bacteria show a résistance too many types of antibiotics, while for the propolis, the bacteria is
resistant to only one extracts. Sow we have founded that propolis extracts is more active than the
antibiotics.
Key words: Propolis, Urinary infection, Pathogeneous bacteria, Antibiotic.
92
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ŕŻŕŮŕŗŧƏŸŬƈƓŬŕŬƉŗž ũŲŦƗŔŧƈţƈũŕſƅŕŗ
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œƒŧřƂŕƃŒŧœƒřŤŒ
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ECBU Examen Cyto-ƀ»ƔũųƉ»ŷƓƆŦŔŧ»ƅŔƋũ»ŗŦƈƓ»žŚŰŦŮƏũœŔŪŠƅŔƉƈƓƁũŮƅŔŖƏƊŠƅŕŗřƆƁũƏŗŽŕƔŲƏŗ
ƓƍƏBactériologique des urines
Entérobacter spžProteus sp ž Providencia sp, ž Escherichia coli žStaphylococcus aureus
ŗƒƍƒšƃŒŘŒťœŰƆƃŒŘŒŧœŕřŤŒ
ų»ŬƏƓ»žŚŕƂƆŸƈ¿ƄŮƑƆŷŕƔũśƄŗƅŔŚŷũŪŜƔţGélose Muller-Hinton (GMH) ųŬƏƅŔƑƆŷŚŔũŕŗśŦƛŔŚƔũŠŌ
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93
Annales de la Faculté des Sciences et Sciences de l'Ingénieur
αΩϧϬϣϟ΍ϡϭϠϋϭϡϭϠόϟ΍ΔϳϠϛΕΎϳϟϭΣ
Vol. 1 N° 3/2009
ůũƂƅŕŗŕƍŪƔƄũśƏŕƎśŔũŕŰśŦŔƏřƆƈŸśŬƈƅŔřƔƏƔţƅŔŚŔŧŕŲƈƅŔ1¾ƍťŞƃŒ
ŧŕ»ŲƈƅŔ řƔƈƄ
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ůũƂƅŕŗ
lOOµg
SPI
20 ug
ƒƏƔţƅŔŧŕŲƈƅŔ ŧŕŲƈƅŔ řƔƈƄ ũŕŰśŦƛŔ
ƒƏƔţƅŔŧŕŲƈƅŔ
Spiramycine
ůũƂƅŕŗ
20µg
AMO
Amoxiline
IMI
Imipnem
05µ.g
OXA
Oxacilline
50µg
COL
Colistine
30µg
PEN
Penicilline
05µg
TRI
Triméthoprime
30µg
CEF
Céfotaxime
15 ug
VAN
Vancamycine
1Oµg
GEN
Gentamycine
300µg
FUR
Furanes
30µg
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Amikacine
30µg
ANA
30µg
CHO
Chloramphénicol
20µg
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Acide
Nalidixique
Cefalixine
30µg
TET
Tetracycline
30µg
RIF
Rifamicine
15 µg
ERY
Erythromycine
15 µg
AUG
Augmentine
15 µg
CEZ
Cefazoline
ŧŕƂŶƃŒŘœŮƄŤřŪƆŧœŕřŤŒ
Gélose Muller-ųŬƏƅŔƑƆŷřžŕŠƅŔƌŗŮƏřžŕŠƅŔƀųŕƊƈƅŔƓžřƔœƔŗƅŔřƈŴƊƗŔřƔŕƈţũŗŦƈƐƏśŬƈƑƆŷŚŔũŕŗśŦƛŔŚƈś
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ƒũ»śŗƀŕŗųŏřŝƜŝƅųŬƏƅŔŢųŬƑƆŷŚŸŲƏƓśƅŔŚŔũųƁ¿ƄŮŗƇƂŸƈƅŔƓƂƊƅŔƉƔũŬƆŠƅŔƓž 1ŪƔƄũśŗŚƊŕƄřƆƈŸśŬƈƅŔ
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ŗŬſœƈƆƃŒƍŝőœřƈƃŒ
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E2žƓƅƏƊŕ»ŝƔƈƅŔůƆŦś»ŬƈƅŔE1 E4žE3žE2 žE1 Śŕ»ŰƆŦśŬƈř»ŸŗũŌƑ»ƆŷŕƊƆŰţś¿ŰſƅŔŚŕƔƆƈŷʼnŔũŠŏŧŸŗ
ƓœŕƈƅŔůƆŦśŬƈƅŔE4žũŝƔƛŔůƆŦśŬƈE3žƓƅƏũśŗƅŔũŝƔƙŔůƆŦśŬƈ
ŗƒƍƒšƃŒŘŒťœŰƆƃŒŘŒŧœŕřŤŔ
72¿ƏŔŧŠƅŔƓžřƊƔŗƈƅŔşœŕśƊƅŔƑƆŷŕƊƆŰţśtƇ37ŘũŔũţřŠũŧƑƆŷƒũśŗƀŕŗųŌƉŲţƉƈřŷŕŬ18ŧŸŗ
řƔŗƆŬŖƔŨƈƅŔŚŔũŕŗśŦŔƉŌřŴţƜƈŶƈƏŖ5žŔ5žŖ4žŔ4ž3ž2ž1¿ŕƄŮƗŔƏ
94
ƓƅƏŗƅŔŪŕƎŠƆƅřŲũƈƈƅŔŕƔũśƄŗƅŔŧŲřƆƈŸśŬƈƅŔřƔƏƔţƅŔŚŔŧŕŲƈƅŔƏ(Propolis)ũŗƄŸƅŔŚŕŰƆŦśŬƈƉƔŗřƊũŕƂƈřŬŔũŧ
ŕŻŕŮŕŗŧƏŸŬƈƓŬŕŬƉŗž ũŲŦƗŔŧƈţƈũŕſƅŕŗ
Enterobactersp.ŕƔũśƄŗŧŲřƆƈŸśŬƈƅŔřƔƏƔţƅŔŚŔŧŕŲƈƅŔũŕŗśŦŔ2¾ƍťŞ
CEF
AU
G
Ƈ
CHO
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TET
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FUR
COL
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AMI
š ƒƏƔţƅŔŧŕŲƈƅŔ
Ƈ š ƒƏƔţƅŔŧŕŲƈƅŔ
X
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X
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X
COL
X
AUG
X
CEZ
X
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X
TRI
X
AMI
X
IMI
X
GEN
X
TET
X
FUR
řŬŕŬţš
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Proteus spŕƔũśƄŗŧŲřƆƈŸśŬƈƅŔřƔƏƔţƅŔŚŔŧŕŲƈƅŔũŕŗśŦŔ2¾ƂŬ
95
Annales de la Faculté des Sciences et Sciences de l'Ingénieur
αΩϧϬϣϟ΍ϡϭϠϋϭϡϭϠόϟ΍ΔϳϠϛΕΎϳϟϭΣ
Vol. 1 N° 3/2009
AMO
Providencia spŕƔũśƄŗŧŲřƆƈŸśŬƈƅŔřƔƏƔţƅŔŚŔŧŕŲƈƅŔũŕŗśŦŔ4¾ƍťŞ
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Providencia sp ŕƔũśƄŗŧŲřƆƈŸśŬƈƅŔřƔƏƔţƅŔŚŔŧŕŲƈƅŔũŕŗśŦŔ3¾ƂŬ
Escherichia coliŕƔũśƄŗŧŲřƆƈŸśŬƈƅŔřƔƏƔţƅŔŚŔŧŕŲƈƅŔũŕŗśŦŔ5¾ƍťŞ
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96
ƓƅƏŗƅŔŪŕƎŠƆƅřŲũƈƈƅŔŕƔũśƄŗƅŔŧŲřƆƈŸśŬƈƅŔřƔƏƔţƅŔŚŔŧŕŲƈƅŔƏ(Propolis)ũŗƄŸƅŔŚŕŰƆŦśŬƈƉƔŗřƊũŕƂƈřŬŔũŧ
ŕŻŕŮŕŗŧƏŸŬƈƓŬŕŬƉŗž ũŲŦƗŔŧƈţƈũŕſƅŕŗ
Staphylococcus aureusŕƔũśƄŗŧŲřƆƈŸśŬƈƅŔřƔƏƔţƅŔŚŔŧŕŲƈƅŔũŕŗśŦŔ6¾ƍťŞ
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Annales de la Faculté des Sciences et Sciences de l'Ingénieur
Vol. 1 N° 3/2009
αΩϧϬϣϟ΍ϡϭϠϋϭϡϭϠόϟ΍ΔϳϠϛΕΎϳϟϭΣ
ůƆŦśŬƈƆƅřƔŬŕŬţƅŔƇŧŷůƆŦśŬƈƆƅřƔŬŕŬţƅŔ
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E1
E2
E2
E3
E3
ũŗƄŸƅŔŚŕŰƆŦśŬƈƅ Proteus spřƔŬŕŬţ7¾ƂŬũŗƄŸƅŔŚŕŰƆŦśŬƈƅ Enterobacter spřƔŬŕŬţ6¾ƂŬ
E1
E1
E3
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ũŗƄŸƅŔŚŕŰƆŦśŬƈƅ Escherechia coliřƔŬŕŬţ9¾ƂŬũŗƄŸƅŔŚŕŰƆŦśŬƈƅ Providencia spřƔŬŕŬţ8¾ƂŬ
E2
E3
ũŗƄŸƅŔŚŕŰƆŦśŬƈƅ Staphylococcus aureus řƔŬŕŬţ10¾ƂŬ
98
E1
ƓƅƏŗƅŔŪŕƎŠƆƅřŲũƈƈƅŔŕƔũśƄŗƅŔŧŲřƆƈŸśŬƈƅŔřƔƏƔţƅŔŚŔŧŕŲƈƅŔƏ(Propolis)ũŗƄŸƅŔŚŕŰƆŦśŬƈƉƔŗřƊũŕƂƈřŬŔũŧ
ŕŻŕŮŕŗŧƏŸŬƈƓŬŕŬƉŗž ũŲŦƗŔŧƈţƈũŕſƅŕŗ
ƒŧ»ŗśůũ»Ɓ49ŕƎƊƈŧŠƏůũƁ59ŕƍŧŧŷźƆŗƓśƅŔƏřƆƈŸśŬƈƅŔřƔƏƔţƅŔŚŔŧŕŲƈƅŔůŦƔŕƈƔžƌƊŌŧŠƊřƂŗŕŬƅŔşœŕśƊƅŔƉƈƏ
ů»ŦƔŕ»ƈƔžŕƈŌ16,95řŗŬƊŗƏŕƎƍ੶ŔřƈƏŕƂƈŕƔũśƄŗƅŔƒŧŗśůŔũƁŌ10Ə83,05řŗŬƊŗƏŕƎƍ੶ŔřƔŬŕŬţŕƔũśƄŗƅŔ
řŗ»ŬƊŗƏŕ»Ǝƍ੶ŔřƔ»ŬŕŬţŕ»ƔũśƄŗƅŔƒŧ»ŗśŘũ»ųƁ13ŕƎƊƈŧŠƏ ŘũųƁ 15ŕƍŧŧŷźƆŗƓśƅŔƏřƆƈŸśŬƈƅŔũŗƄŸƅŔŚŕŰƆŦśŬƈ
24,44řŗŬƊŗŕƎƍ੶ŔřƈƏŕƂƈŕƔũśƄŗƅŔƒŧŗśƉƔśũųƁ Ə86,66
ŧŔƏ»ƈƅŔƉ»ƈũƔŝƄƅŔƑƆŷƌœŔƏśţƛŔũŴƊŕƎƊƈũƔŗƄŧŧŷƏƈƊŢŗƄƔƏŕƔũśƄŗƆƅŧŕŲƈƄƌŰœŕŰŦŗŽƏũŸƈũŗƄŸƅŔƏŧƔŸŗƉƈŪŨƊƈ
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ű»Ÿŗŧ»ŲŚƆƈŸśŬŔƓśƅŔƏũœŔŪŠƅŕŗřƆŮƊŦřƂųƊƈƉƈŵƏƈŠƈƅŔũŗƄŸƅŔŚŕŰƆŦśŬƈřŝƜŝƑƆŷŕƊƆŰţśƋŨƍŕƊśŬŔũŧƓžƏ
ŵŔƏ»ƊƗŔ¿»ƄŧŲřƔƆŷŕžũŝƄŌE3ƏE1ƉƔŰƆŦśŬƈƅŔƉŌŚƊƔŗ¿ƏŧŠƅŔƓžřƊƔŗƈƅŔŚŔũŕŗśŦƛŔƓƅƏŗƅŔŪŕƎŠƆƅřŲũƈƈƅŔŕƔũśƄŗƅŔ
Staphylococcus aureusž Escherichia coliƏƈƊŢŗƄŜƔţřŰŕŦřŠƔśƊƑųŷŌE2ůƆŦśŬƈƅŔŕƈŌŘũŗśŦƈƅŔřƔũśƄŗƅŔ
1993žkrol et al. ƌƅ¿ŰƏśŕƈŶƈƀžŔƏśƈŔŨƍƏ Proteus spž
ŕ»ƈŌ 24,44ř ŗ»ŬƊŗřƈƏŕƂƈƏ 86,66řŗŬƊŗũŗƄŸƅŔŚŕŰƆŦśŬƈƅřŬŕŬţŚƊŕƄŕƔũśƄŗƅŔƉŌƉƔŗśŕƎƊƔũŠŌƓśƅŔřŬŔũŧƅŔƓž
16,95řŗŬƊŗřƈƏŕƂƈƅŔƏ83,05řŗŬƊŗřƔŬŕŬţƅŔŚƊŕƄžřƔƏƔţƅŔŚŔŧŕŲƈƅŔůŔũƁƗřŗŬƊƅŕŗ
řƆƈŸś»ŬƈƅŔř»ƔƏƔţƅŔŚŔŧŕŲƈƅŔƉƈřƔƆŷŕžũŝƄŌƒŧŗƔũŗƄŸƆƅƓƅƏƊŕŝƔƈƅŔůƆŦśŬƈƅŔƉŌƉƔŗśŕƎŗŕƊƈƁƓśƅŔŖũ੶ƅŔ¿ƜŦƉƈƏ
ƓƅƏŗƅŔŪŕƎŠƆƅřŲũƈƈƅŔŕƔũśƄŗƅŔŧŲ
ŴŞŒŧƆƃŒ
Ary Fernandesm J., Elaine C. B., Joyce E., Cristina B., Ricardo D. O., Maria de Lourdes R. S. C.,
Augusto C. M. ; 2005. Propolis: anti-Staphylococcus aureus activity and synergism
withantimicrobial drugs Mem Inst Oswaldo Cruz, Rio de Janeiro, 100(5) : 563-566
Avril J.L. et Fauchere J.l. ; 2OO2. Bactériologie générale et médicale, Ed : Ellipses, Paris. PP:141142
Bankova V.,Christov R.; Popov S., Pureb O. and Bocari G.; 1994. Volatile constituents of propolis
eitschrift fur Naturforschung, 49c :6-10
Bankova V., Christov R., Kujumgiev A.,Marcucci M. C. and Popov; 1995 .Chemical composition
and antibacterial activity of Brazilian propolis. Zeitschrift fur Naturforschung. 50c: 167–172
Bankova V., Christov R. and Tejera A. D.; 1998. Lignans and other constituents of propolis from
the Canary islands. Phytochemistry. 49:1411-1415
Bankova V., Christov R., Popov S., Marcucci M. C., Tsvetkova I and Kujumgiev A.; 1999.
Antibacterial activity of essential oils from Brazilian propolis. Fitoterapia. 70: 190–193
Bosio K., Avanzini C., D’avolio A., Ozimo O., Savoia D. ; (2000). In vitro activity of propolis
against Streptococcus pyogenes. Lett Appl Microbiol 31: 174-177.
99
Annales de la Faculté des Sciences et Sciences de l'Ingénieur
Vol. 1 N° 3/2009
αΩϧϬϣϟ΍ϡϭϠϋϭϡϭϠόϟ΍ΔϳϠϛΕΎϳϟϭΣ
Kusumoto T., Miyamoto R.H., Doi S., Hiroyuki S., Yamada H.; 2001. Isolation and structures of
two new compounds from the essential of Brazilian propolis. Chem Pharm Bull 49:
Burdock G.A.; (1998). Review of the biological properties and toxicity of bee propolis (Propolis).
Food Chem Toxicol 36: 347-363
Hyun K., Pedro L. R.; Jaime A. C., Yong K. P. and William H. B.; 2002. Effects of Compounds
Found in Propolis on Streptococcus mutans Growth and on Glucosyltransferase Activity.
Antimicrobial agents and chemotherapy . 46:1302-1309
Paul S. ; 1999. Bactériologie . 4éme édition Dunod, Paris. PP : 331-332
Pilet C., Bourdon J.L., Marchal N. et Balbastere C. ; 1983. Bactériologies médicale et vétérinaire
systématique bactérienne. Masson, Paris. PP : 141, 161, 164, 169
100