Antibodies for Cancer research fra CST, Nyt NEB
katalog med fine giveaways, L7 hPSC Culture
System fra Lonza samt nye produkter fra NEB
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Stem Cell Transfection using
Nucleofector™ Technology
L7™ hiPSC Reprogramming and
hPSC Culture System
Transfection of stem cells is typically a very difficult
task using non-viral methods. With the
Nucleofector™ Technology stem cells can be
consistently transfected at high efficiency for
various applications, comprising those that require
co-transfection of several substrates:
The L7™ hPSC Culture System is a robust culture
system that supports every other day feeding of
pluripotent stem cells under defined and xeno-free
conditions. This xeno-free, fully defined system
includes a medium, matrix, passaging and
cryosolution. L7™ is a complete system for the
maintenance and expansion of human embryonic
stem cells (ESCs) and induced pluripotent stem
cells (iPSCs).
• Proven for genome editing in ESCs or iPSCs
using ZFN, TALEN, and CRISPR systems
• Efficient episomal reprogramming of PBMCs,
fibroblasts, CD34+ or other cells into iPSCs
• Optimized, ready-to-use transfection protocols
for primary adult stem cells such as mesenchymal
stem cells (MSCs)or neural stem cells (NSCs)
• Reliable reprogramming of peripheral blood
mononuclear cells (PBMCs) into iPSCs
• Robust culture system that supports every other
day feeding of pluripotent stem cells
• Efficient differentiation into all three germ layers
• Easy transition to clinical applications xeno-free, defined
View archived webinar and learn about genome
editing in hard-to-transfect cells
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Find out how to easily generate induced
pluripotent stem cells from PBMCs or other cells
using the Nucleofector™ Technology.
NYHED!
NEB® Golden Gate
Assembly Mix
Did you know that NEB’s restriction enzymes and
ligases can be used for Golden Gate Assembly, a
technique for the efficient and seamless assembly
of DNA fragments? Our new Golden Gate
Assembly Mix incorporates digestion with BsaI and
ligation with T4 DNA Ligase into a single reaction,
and can be used to assemble up to ten fragments
in a single step.
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NYHED!
Rapid PCR Cleanup Enzyme Set
NEB Rapid PCR Cleanup Enzyme Set consists of
two recombinant enzymes, Exonuclease I (Exo I,
#M0293) and
Shrimp Alkaline Phosphatase (rSAP, #M0371). It
allows for rapid and complete enzymatic
degradation of residual PCR primers and
dephosphorylation of dNTPs subsequent to PCR.
The Rapid PCR Cleanup Enzyme Set enables
direct downstream applications including
sequencing, genotyping, cloning or SNP analysis
without the need for additional purification steps.
The Rapid PCR Cleanup Enzyme Set is added
directly to the PCR product after thermal cycling
and is 100% compatible with commonly used PCR
reaction buffers.
Bestil gratis prøve hos BioNordika!
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NYHED!
Antibodies for Cancer Research:
PD-L1, PD-1, OX40, OX-40L, CTLA-4,
CD40L validated for Flow and IHC
• High Sensitivity: detect endogenous levels of target protein
expression in human tissues or primary human cells
• Target Specificity: no cross-reactivity and tested in relevant
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PBMC models
• Monoclonal Antibodies: ensure lot-to-lot consistency
• Optimized Protocol: provided for key applications
• Custom Formulations available: for labeling and platform specific applications
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CD40 Ligand (D5J9Y)
Rabbit mAb #15094
PD-L1 (E1L3N®)
XP® Rabbit mAb #13684
• CD40 ligand is expressed on T cells and
activates signaling cascades such as MAPK and
NF-κB when bound to CD40
• WB, IP, and IHC-P validated
• PD-L1 (B7-H1, CD274) inhibits activated T cells
by binding to its receptor, PD-1, on the surface of
activated T cells.
• IHC, flow, WB, IP and IF validated
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Se flere PD-L1 mAb varianter
PD-1 (D3O4S) Rabbit
mAb & Conjugates
• PD-1, expressed on T cells,
regulates immune tolerance by
suppressing T cell signaling when
bound to PD-L1 or PD-L2
• Flow cytometry validated
Se PD-1 mAb varianterne her
NEBNext® Ultra™ DNA Library
Prep Kit for Illumina®
NEBNext® Ultra™ RNA Library
Prep Kit for Illumina®
When determining which NEBNext product to
NEBNext Ultra kits are available for directional
(strand-specific) or non-directional library
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choose, you’ll need to
decide whether to use the
Ultra or Standard
workflows. Ultra
workflows are faster, have
fewer components and
clean-up steps, and are
optimized for lower input
amounts. For DNA, the
Ultra workflow library
preparation is complete in 2.5 – 3 hours, with only
15 minutes of hands-on time. The Ultra workflow is
optimized for input amounts between 5 ng and 1
µg and is also ideal for ChIP DNA. When time is of
the essence and starting material is precious, Ultra
kits ensure optimal yields.
preparation. These kits
utilize streamlined
workflows and novel
master mixes, and have
been designed for
performance with input
amounts as low as 10 ng
(purified mRNA or rRNA –
depleted RNA) for
Directional RNA and
non-directional RNA. Standard non-directional
workflows are enabled by our standard kits, which
support input amounts above 50ng.
Our novel Small RNA workflow has been
optimized to minimize adaptor-dimers, while
producing high-yield, high-diversity libraries.
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SE HER!
Bestil det nye NEB 2015/16 katalog hos BioNordika
og få et minutur, en floatie, en nøglering og en blyant fra NEB!
Eller bestil på mail [email protected]
BioNordika Denmark A/S - Marielundvej 48, 1. - 2730 Herlev - 3956 2000 - [email protected] - Afmeld
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