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V-600 Series Accessories
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Type 01
1
V-600 Series
UV-Vis/NIR Spectrophotometers
The Jasco V-600 series of UV-Vis/NIR spectrophotometers feature
the latest technology offering high measurement accuracy and
stability. With the ability to incorporate multiple, exchangeable
accessories for various applications, the V-600 is easy to operate
and offers a wide range of capabilities.
There are five models available: the compact, high-performance
V-630, the V-630Bio, the V-650 with a single monochromator, the
V-660 with a double monochromator, and the high-throughput
V-670 operating into the NIR spectral range.
Contents
2V-600 Series UV-Vis/NIR
Spectrophotometers
15V-600 Series Optional Accessories
for Liquid Samples
30
-600 Series Optional Accessories
V
for Solid Samples
4
IRM-700 Intelligent Remote Module
15Cell Holders Used at Ambient
Temperature
31
Film Holders
33
Specular Reflectance Accessory
5
Spectra Manager II & CFR
16Constant Temperature Cell Holders
Used at Ambient Temperature
35
Integrating Spheres
6
Optional Software Packages
17
Peltier Thermostaffed Cell Holders
39
Optical Fiber Probe Units
18
Micro Cell Holders
6Multivariate Quantitative Analysis
Software Packages
7
Color Analysis Software Packages
19Flow System, Flow Cell, Sipper,
Autosampler
10
Biochemical Software Packages
28
12Materials Analysis Software Packages
Liquid Samples Chart
40Absolute Reflectance Measurement
Accessory
For each model, choose between the iRM
intelligent remote module or a DS type PC
control. Select the type of control according
to measurement purpose, data analysis
requirements and available bench space.
With iRM control, the spectrophotometer
can also be connected to a PC with the
Spectra Manager software for extended
instrument control and data analysis.
Conversely, with the DS type instrument, an
iRM can also be used for more immediate
instrument control.
The iRM controlled instrument offers
operability and analysis features that
exceed the bounds of existing integrated
control systems. The compact lightweight
unit offers easy operations characterized
by earlier iRM modules that can now be
performed using a touch panel interface
with a high visibility, color liquid crystal
display. Smooth operation is ensured by
operational icons, pull down menus for
optional settings, confirmation messages
and alphabet/numeric key displays.
Measurement results can be saved in
a CompactFlash memory in either the
standard JASCO format, or in text format for
easy transfer to a PC. Instrument results can
be output to an inkjet printer equipped with
a PictBridge interface, offering color outputs
for records with standardized, attractive
layouts.
For PC control, JASCO Spectra Manager
—the common software platform for all
JASCO spectroscopy instruments—offers
smooth operability through all steps from
data measurement to advanced analysis
applications. Spectra Manager CFR is
also available for compliance with 21
CFR 11 regulations. A USB 2.0 interface
for connection to a PC offers high-speed
communication with no requirement for PCI
board expansion.
V-630
• Compact, low cost,
high accuracy, high
speed scanning (8000
nm/min.) USP/EP
compliance
V-630Bio
• Compact, low cost,
high accuracy, high
speed scanning (8000
nm/min.) USP/EP
compliance
V-650
• Single monochromator
A wide range of optional accessories and software programs
for liquid and solid samples.
The V-600 Series comes with a complement
of more than 50 accessories and over
20 optional programs. The V-600 series
offers flexible support for a wide range
of analysis from specific applications
of advanced research labs to simple
educational applications and routine daily
use, with measurement techniques for
variously shaped samples, such as cells for
measurement of liquid samples of 5 μl, and
integrating spheres for directly measuring
large samples which do not fit in the sample
chamber and which cannot be cut.
with low stray light
(Abs 4)
• Accepts external
detectors (integrating
sphere or absolute
reflectance
measurement)
• Easily accepts micro
cells
V-660
• Double monochromator
IQ Accessory and IQ Start
Information concerning sample
measurement consists of various elements
such as the measurement method, data
collection parameters, type of sample and
user/sample information. The IQ Accessory
and IQ Start features capture measurement
parameters, linking the measurement
program and measurement data to the
accessories.
IQ Accessory (automatic accessory
recognition) recognizes that the sample
chamber is fitted with a corresponding
accessory; the name of the accessory,
model and serial number are attached
to the measurement data as accessory
information for all data files. Furthermore,
IQ Start links to an IQ Accessory and if an
with low stray light
accessory with a pre-registered program
is attached, the registered program is
initialized. This capability is very convenient
for routine analysis since the designated
analysis starts up by the simple step of
placing an accessory into the instrument.
• Expanded shorter
wavelength range (down
to 187 nm)
V-670
• NIR range (up to
2700 nm)
• Expandable wavelength
range up to 3200 nm
option
• Integrated PbS power
supply
3
iRM-700 Intelligent Remote Module
User-Friendly Graphical Interface
Main Features and Standard Functions
The iRM-700 combines a color LCD touch
panel with an interface using icons and
windows to offer sophisticated operability
with a compact design. The iRM offers
a pleasant analysis environment that
overturns the normal expectations of typical
small and integrated modules. The color
display improves visibility, for an attractive
interface. Eliminating a keyboard in favor
of a touch panel display enables a more
compact module with smooth operation.
The operation keys are displayed in a
format that suits each task and operations are performed by simply touching the screen. By
displaying the operation keys as icons, error and confirmation messages in dialog boxes and
options in a pull-down format, a full range of functions can be accessed while reducing the
complexity of standard operation. The screen can be easily switched between vertical and
horizontal display for expanded spectral display.
4
IQ Accessory
IQ Start
When an IQ Accessory is placed into the
instrument sample compartment, the
accessory information (model, name and
serial number) and specific parameters for
the accessory, registered in an on-board
chip, are downloaded to the software via
the main unit. The accessory information is
displayed on the measurement screen and
is recorded with the measurement data. If
the accessory is attached before turning
on the power, the accessory information is
downloaded after instrument initialization.
The IQ Start function is integrated with an
IQ Accessory; when an IQ Accessory is
placed in the sample compartment, a
preregistered measurement program starts
automatically. With the iRM, combinations of
accessories and programs can be registered
in two ways and up to two measurement
programs can be registered for each
accessory. When the accessory is attached,
a dialog box for selection of the registered
program is displayed. Providing convenience
for routine analysis, frequent measurements
with a specific parameter set can be utilized
for smooth measurement capability.
Parameter Settings and Accessory Control
Using IQ Accessory, an accessory for the V-600 is automatically detected and the default
parameters are set. The following accessories can be controlled by the iRM.
Cell changer
NCP-511, NCP-705, NCP-508, NCP-706
Peristaltic sipper
NPF-509, NPF-721
Vacuum sipper
NQF-610, NQF-720
Temperature controller
ETC-505, ETC-717, EHC-477, EHC-716
Peltier cell changer
PSC-498, PSC-718, PAC-743, PAC-743R
External detector
ARV-474, ARV-730, ARN-475, ARN-731, ARV-474S,
ARSV-732, ARN-475S, ARSN-733, ISV-469, ISV-722,
ISN-470, ISN-723, ILV-471, ILV-724, ILN-472, ILN-725,
IJV-606, IJV-726, IJN-607, IJN-727, HIS-612, HISV-728,
HIS-613, HISN-729, PIV-756, PIN-757, FAV-750, FAN-751
Note 1: External detectors are not compatible with the V-630/V-630Bio instruments.
Note 2: Automatic absolute reflectance accessories cannot be controlled using the iRM.
Spectra Manager II & CFR
User-Friendly Graphical Interface
Main Features and Standard Functions
JASCO Spectra Manager II is an integrated
software package for cross-platform
control of all JASCO spectroscopy
instruments, including UV-Vis, Near-IR, FTIR, fluorescence, Raman, polarimetry and
Circular Dichroism spectrometers. Spectra
Manager II software provides functionality
ranging from analytical instrument
control and spectral data processing to
sophisticated, specialized data analysis
programs. JASCO Spectra Manager CFR
provides enhanced security and auditing
functions that ensures the security, integrity
and confidentiality of electronic records by enforcing the use of electronic signatures and
other security measures as described in the regulations outlined within 21 CFR Part 11.
5
Optional Software Packages
Spectra Manager II
Multivariate Quantitative Analysis Software Packages
For samples where the absorption band of each component of a multicomponent system
cannot be separated, methods of quantitation using multivariate analysis without the use
of separation techniques like chromatography are widely used. The relationship between
the spectrum and the density or physical quantity of the components is computed using
mathematical and statistical techniques, enabling highly precise quantitation.
VWCL-782 CLS Quantitative Program
Classical Least Squares
The CLS quantitation program calculates the virtual spectrum of each component in its
pure form from the spectra of standard samples and the concentration information of all the
components using the least-squares method. Using a calibration model calculated from the
spectra of the pure components, batch quantitation is performed. This program is effective
when all the components in the multi-component spectrum are known. Since the CLS
program examines the contribution to the spectral interaction between each component, this
method is suitable for quality control of manufactured products.
Structure:
Calibration model creation program, quantitative
analysis program
Number of quantitation
components:
Maximum 16 components
Number of standard samples for
creation of calibration curves:
Number of components + 1 (minimum) to (2 x number
of components) or more
Calculated wavelength range:
Maximum of four ranges can be selected
Calculation result table:
Maximum of 100 prediction results
VWPC-783 PCR Quantitative Program
Principal Components Regression
The PCR quantitation program performs principal component analysis for multiple standard
spectra. It creates a calibration model for the results and for changes in the concentration or
physical amount of the target components, and performs quantitative analysis of unknown
samples.
Besides being used for evaluation of manufactured products such as food or polymers, it is
recently also finding applications in noninvasive analysis of cosmetics and similar products.
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Structure:
Calibration model creation program, quantitative
analysis program
Number of quantitation
components:
Maximum 16 components (calibration models are
created individually)
Number of standard samples for
creation of calibration curves:
Minimum 8 samples (20 samples or more are
appropriate)
Calculated wavelength range:
Maximum of four ranges can be selected
Calculation result table:
Maximum of 100 prediction results
VWPL-784 PLS Quantitative Program
Partial Least Squares
The PLS quantitation program creates
calibration models from changes in the spectra
of standard samples and from the concentration
information or physical amount of the target
components.
Spectrum components which depend on density
(factor loading vector) are sampled from the
standard spectrum using the least-squares
method, and regression is performed on the
scores obtained and the density. Next, a similar calculation is performed repeatedly with
the residual of the spectrum and the density until the error becomes sufficiently small to
quantitate the target component.
This is suitable for measurement of samples where it is difficult to examine all the
components, such as food and related products.
Structure:
Calibration model creation program, quantitation
program
Number of quantitation
components:
Maximum 16 components (calibration models are
created individually)
Number of standard samples for
creation of calibration curves:
Minimum 8 samples (20 samples or more are
appropriate)
Calculated wavelength range:
Maximum of four ranges can be selected
Calculation result table:
Maximum of 100 prediction results
VWPA-785 PCA Quantitative Program
Principal Component Analysis
Principle Components Analysis (PCA) is
used to sample characteristic spectral
patterns from sample spectra to create
reference spectra divided into groups,
known as a PCA model. This model can
then be used to predict to which grouping
of spectra an unknown sample spectrum
belongs. PCA can be used for acceptance
inspections of pharmaceuticals and for
quality inspection of foods, polymers, paints, etc. where there may not be clear distinctions
between the sample spectra.
Structure:
PCA model creation program, PCA prediction
program
Number of principal components:
2~10 components
Number of samples for creating models:
Minimum (principal components + 1)
Calculated wavelength range:
Maximum of four ranges can be selected
Optional Software Packages continued
7
Optional Software Packages
Spectra Manager II
Color Analysis Software Packages
VWCD-790 Color Diagnosis Program
The Color Diagnosis Program calculates colors of samples using the various color systems
established by the CIE (International Commission on Illumination) using the spectrum from
380-780 nm. The display method for object colors using the various color systems is based
on JIS/ISO requirements. With a function for judging pass/fail requirements with regard to a
reference value set, the program is suitable for product evaluation and quality control. The
pass/fail criteria can be set as a color difference; a measured spectrum can be loaded and
a range set for the chromaticity coordinates; or specific numeric values for the chromaticity
coordinates can be input directly for comparison. Setting the color calculation conditions
is achieved by simply following the directions in the ‘wizard’ setup dialogs. When the color
system is selected, the related color calculation items are displayed and only the required
items can be selected. The conditions for color difference calculations are set by entering
a number for the tristimulus values of the standard sample, or by specifying the measured
standard spectrum. When spectral measurements are complete, the various values for
the color calculations and the pass/fail judgement are displayed in the result table. The
chromaticity diagram for the selected color system are displayed in the CIE color diagram so
that the coordinates of the measured sample are easily recognized. In addition, it is possible
to retrieve several measured spectra and perform a batch calculation for the multiple spectra.
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Light source:
A, D65, C, B, user-defined light source
Viewing angle:
2º, 10º
Wavelength calculation range:
380~780 nm
Calculation data interval:
5 nm, 10 nm
Result table:
Maximum 100 files
Available color system
calculations:
XYZ (JIS Z8701), L*a*b* (JIS Z8729), Lab, Munsell (JIS
Z8721), L*u*v* (JIS Z8729)
Color calculation items:
Tristimulus value: XYZ; chromaticity coordinate: xy;
whiteness level (JIS Z8715), yellowness level: YI;
luminosity, hue, and color for each color system;
chromaticity coefficient; hue angle; color difference;
dominant wavelength: λd; pure stimulus value: Pe;
and pass/fail judgement.
VWLU-788 Luminous Color Measurement Program
The Luminous Color Measurement Program calculates the color of illuminants such as light
sources, light emitting diodes (LED) and other external light devices. Results can be plotted
in a chromaticity diagram and pass/fail judgements can be performed according to the
standard values set by the user. The program also contains 15 standard spectra.
Additional accessories such as the ELM-742 external light input accessory and a calibrated
light source for instrument correction are also required for optimal calculation.
Viewing angle:
2º, 10º
Wavelength calculation range:
380~780 nm
Calculation data interval:
5 nm
Result table:
Maximum 100 files
Available color system
calculations:
X Y Z (Y is relative luminance), Luv, Lu’v’ (JIS Z8701)
Chromaticity coordinates:
(x, y), (u, v), (u’, v’)
Calculation items:
Tristimulus value: XYZ; dominant wavelength λc
(complementary wavelength λc); related color
temperature Tcp and deviation duv (JIS Z8725); color
rendering index Ra, R1~R15 (JIS Z8726); fluorescent
lamp light source color classification (JIS Z9112), and
pass/fail judgement.
VWAC-796 ASTM Color Analysis Program
This program calculates the ASTM color for petrochemical products such as lubricating
oil, diesel and heating oils using the ASTM and JIS K2580 standards. Using the XYZ color
system, the tristimulus values XYZ and the sum ∑D of the optical density are calculated from
the measured spectrum and the ASTM color is calculated based on the results.
A long-path cell holder accessory may also be required depending on the sample type.
VWCM-795 Color Matching Program
This program offers capabilities for standard color library management and Computer
Color Matching (CCM) for transparent colors. Standard color library management provides
the registration of standard color pigment spectrum files in a library. The CCM function
can perform color mixing calculations from spectra of target colors. Using the CCM
program, a target color and reference color library are selected, then the mixed color can
be automatically calculated after selecting the minimization parameters for an ‘unknown’
spectrum including the ∆XYZ, ∆E*ab, the light source, viewing angle and sample preparation
parameters.
VWSC-797 Saybolt Color Analysis Program
This program uses the Saybolt color measurement standard (JIS K2580) for petrochemical
products such as kerosene, gasoline and other fuels. Using the XYZ color system, the XYZ
tristimulus values, luminosity L*, chromaticity coefficient a* and b*, and the color difference
∆E*ab values are calculated for the measured spectrum vs. the standard to provide the
Saybolt color value based on the calculated results.
A long-path cell holder accessory may also be required depending on the sample type.
Optional Software Packages continued
9
Optional Software Packages
Spectra Manager II
Biochemical Software Packages
VWKN-772 Kinetics Analysis Program
The Kinetics Analysis Program performs advanced kinetics analysis of multiple sample
kinetics (time course) spectra in which matrix solutions of various analyte concentrations are
added to enzyme solutions, plots the graphs and calculates the maximum reaction velocity
(Vmax), Michaelis Menten constant (Km) and the Hill constant (n). The program also supports
calculation of inhibitor constants and four types of calculations can be selected including
competitive, non-competitive, mixed and uncompetitive. An automated cell changer can be
utilized, enabling batch analysis of multiple data.
Plots: Five types; Michaelis-Menten, Lineweaver-Burk, Hofstee, Eadie, and Hill
Calculation items: Maximum reaction velocity (Vmax), Michaelis-Menten constant (Km), Hill
constant (n), determination of inhibitor type, and inhibitor constant
Supplied standard with
the V-630Bio model
VWTP-780 Temperature Gradient Measurement and DNA Melting Analysis
Program
The Temperature Gradient Measurement program provides the measurement and analysis
for DNA or protein melting experiments. Controlling the temperature of the Peltier accessory
(single or multi-cell), the software provides measurement of the absorbance at a specific
wavelength during temperature changes, then calculates the fusion temperature (Tm) from
the results of the measurement.
Accessories such as the ETC-717 watercooled Peltier cell holder or the PAC -743/
PAC-743R (among others) water-cooled
Peltier cell changers are also required.
10
VWPN-786 Protein Nucleic Acid Quantitation Program
The Protein Nucleic Acid Quantitation program measures the absorbance of protein/nucleic
acid solutions at designated wavelengths and calculates the concentration of the protein and
nucleic acids based on a calculation method selected from five different types.
Alternatively, a user-specified wavelength can be selected using background correction with
the ability to turn on or off the background correction. Correction can be performed for turbid
solutions at a wavelength of 320 nm. Dilution rate correction is also possible with userdefined concentration calculations.
Available calculation methods include: Absorbance ratio of 260/280 nm; absorbance ratio of
230/280 nm; Warburg-Christian method; a user-defined absorbance ratio; and a user-defined
concentration calculation.
Provided standard with the V-630Bio instrument.
VWIS-778 Interval Scan Measurement Program
The Interval Scan Measurement program provides the ability to measure the spectral scans
of samples automatically with a user-defined time interval between scans. The final data
array can be displayed as a 2-D spectral display; a 3-D spectral display; contour, colorimage or cross-section images; or 2-D displays can be provided for results using the peak
height/ratio, peak area/ratio, FWHM or peak shift calculations. An automated cell changer
accessory can also be used for spectral data collection.
VWTS-779 Temperature Interval Scan Measurement Program
The Temperature Interval Scan Measurement program provides the ability to measure the
spectral scans of samples automatically with a user-defined temperature interval between
scans. The final data array can be displayed as a 2-D spectral display; a 3-D spectral
display; contour, color-image or cross-section images; or 2-D displays can be provided for
results using the peak height/ratio, peak area/ratio, FWHM or peak shift calculations. An
automated cell changer accessory can also be used for spectral data collection.
Accessories such as the ETC-717 water-cooled Peltier cell accessory or the PAC -743/PAC743R water-cooled Peltier cell changers are also required.
Optional Software Packages continued
11
Optional Software Packages
Spectra Manager II
Materials Analysis Software Packages
VWML-791 Multi Layer Film Thickness Analysis Program
The Multi-Layer Film Thickness Analysis program calculates the refractive index, absorption
coefficient and film thickness of single or multi-layer films using an absolute reflectance
method, utilizing the refractive index profile of the sample. Film thicknesses can be
calculated for a maximum of five layers. Standard substrate samples are selected for the
model and for each layer from an optical constant library; the refractive index, absorption
coefficient and film thickness of the ‘unknown’ samples are calculated by application of the
least squares method to the sample spectra and the expected film thickness values. The
criteria for film thickness calculations is 50 to 1000 nm.
Any of the ARV-730/ARN-731/ARSV-732/ARSN-733/ARMV-734/ARMN-735 absolute
reflectance measurement accessories is also required.
Calculation items:
Film thickness, refractive index, and absorption coefficient
(mean, chromatic dispersion)
However, the refractive index and absorption coefficient can only
be calculated for single layer films
Calculation method:
Least-squares method
Setting parameters:
Angle of incidence, calculated wavelength range, number of
layers, expected film thickness, and optical constant library
Number of layers:
Maximum five layers (when the surface of the sample is flat)
Optical constant library:
Insulators, semiconductors, metals, polymers (more data can be
added by the user)
VWAL-799 Average Reflectance Calculation Program
The Average Reflectance Calculation program provides the calculation of
the mean measured reflectance (%R), Transmittance (%T) or absorbance
(Abs) spectrum. This analysis program can be used for the evaluation of
glass or film samples using the mean reflectance of a certain wavelength
range, or for evaluating optical components containing an interference
pattern due to a film coating.
Accessories such as the SLM-736/737/738 single reflection measurement
accessory or an absolute reflectance accessory such as the ARV-730/
ARN-731 are also required.
VWRR-769 Reflectance Correction Program
The Reflectance Correction program can convert the relative reflectance spectrum, obtained
by using the single reflection measurement accessory (SLM-736/737/738), to an absolute
reflectance spectrum using a calculation with a correction coefficient. The program also
includes typical absolute reflectance data for an evaporated aluminum mirror. The absolute
reflectance spectrum of the sample can be registered for use as a reference when using a
reference material other than an evaporated aluminum mirror (provided as standard with the
SLM accessory).
The SLM-736/737/738 single reflection measurement accessory is also required.
12
VWAS-793 Variable incident Angle Measurement Program
The Absolute Reflectance accessory with the Variable Incident Angle Measurement program
can perform variable incident angle measurements at a fixed wavelength in conjunction with
the automated absolute reflectance measurement accessory. The program and accessory
support sample measurement at any wavelength within the spectral range of the instrument/
accessory combination.
The program can provide calculation of the refractive index (n) and the extinction coefficient
(k) for samples based on the angle variation data for the reflectance data measured with this
program. Angle variation data is created from the sample measurement data; the refractive
index and extinction coefficient data of the samples are subsequently calculated using the
least squares method from the measured and calculated interference pattern information
obtained from the sample.
The ARMV-734/ARMN-735 automatic absolute reflectance measurement accessory is also
required.
VWAS-794 Phase Contrast Measurement Program
Utilizing the Phase Contrast Measurement program, included as part
of the PDU-755 phase contrast measurement accessory, the reflection
and transmission phase difference for a sample can be measured. The
PDU-755 phase contrast measurement accessory integrates an analyzer
and a phase contrast measurement program. The phase difference (∆)
is calculated from the ratio of the photometric value (I-45/I+45) when the
analyzer is set at +45° and the polarizer is set at ±45° using the polarizer
orientation method. The photometric value is calculated when the
polarizer is set at +45° and the analyzer is set at ±45° using the analyzer orientation method.
One of the ARV-730/ARN-731/ARSV-732/ARSN-733/ARMV-734/ARMN-735 absolute
reflectance measurement accessory is also required. This program is not sold separately.
Calculation method: Polarizer orientation method, analyzer orientation method
VWSP-787 SPF/PA Calculation Program
The SPF/PA Calculation program calculates the SPF value, expressing the degree of
protection against UV exposure, and the PA classification by applying a weighting factor
to each wavelength in the spectrum of a sample. This program allows the evaluation of the
ultraviolet protection effects of clothing, cosmetics, sunscreen formulations, etc., quickly and
at a low cost using a spectrophotometer.
The ISV-722/ISN-723 integrating sphere accessory is also required. A special cell is available
(sold separately) for evaluation of cosmetics and other creams or lotions. The cell has
grooves in a quartz plate which allows samples to be applied with a thickness of 20 μm.
VWST-774 Solar/Visible Light Measurement Program
The Solar/Visible Light Measurement program calculates the visible and
solar transmittance or reflectance of a single glass plate in accordance
with the transmittance, reflectance and solar radiation gain rate test
method for flat glass (JIS R3106). A weighting factor is applied to the
measured spectrum to calculate the solar transmittance, solar reflectance,
visible light transmission, visible light reflection or visible light absorption.
The results of the calculation can be printed with the sample spectrum.
Accessories such as the ISV-722/ISN-723 integrating sphere accessory,
the ARSV-732/ARSN-733 absolute reflectance measurement accessory
or similar are also required. For calculating solar transmittance and
reflectance, a spectrum measurement with a spectral range of 300 to
2,500 nm is necessary. For calculating visible light transmission and reflectance, spectrum
measurement with a spectral range of 380 to 780 nm is necessary.
Optional Software Packages continued
13
Optional Software Packages
Spectra Manager II
Materials Analysis Software Packages
VWSE-798 UV Shield Factor Calculation Program
The UV Shield Factor Calculation program calculates the shield factor
(shield factor = 100 - transmittance) indicating the amount of light
blocked in a certain wavelength region. A maximum of five regions can
be specified for the entire spectrum wavelength range. This program can
be used for calculations of the shield factor of materials that block UV
radiation.
VWHZ-775 Haze Measurement Program
The Haze Measurement program provides a procedure for the
measurement and calculation of the haze value (cloudiness) in
accordance with the JIS K7136 method for plastic and transparent materials. A pass/fail
function is provided, making the program suitable for quality control and product evaluation.
The ILV-724/ILN-725 150 mm diam. integrating sphere accessory is also required.
VWWQ-789 Chromaticity/Turbidity Measurement Program
The Chromaticity/Turbidity Measurement program measures the turbidity and chromaticity
for a sample based on the drinking water test method. The program tests turbidity with
the diffuse transmission measurement at 660 nm and the chromaticity at 390 nm. The
procedures for creation of analytical calibration curves using standard samples and the
quantitative measurement of ‘unknown’ samples are displayed in software dialogs, providing
simple operation.
The ISV-722/ISN-723 integrating sphere accessory is also required.
An optional color diagnosis program is also required for displaying chromaticity using the
calculated chromaticity coordinates.
VWSQ-776 Spectral Quantitative Analysis Program
The Spectral Quantitative Analysis program provides the quantitative analysis for a
maximum of ten peaks by applying the Bouger-Beer law to each analyte peak. Simultaneous
determination of samples with several components is possible if the analyte absorption
peaks from each component do not overlap. Two calculation methods are available, using
either the peak height or peak area of the absorption peak.
VWBG-773 Band Gap Analysis Program
The Band Gap Analysis program calculates the electronic band gap
from the transmission or reflectance spectra of semiconductor materials.
The calculation range of a band gap can be specified interactively
while examining the spectrum. Four calculation methods are available
according to the type of transition such as a direct or an indirect
transition.
Calculation method: Direct transition, indirect transition (Permitted /
Forbidden)
14
V-600 Series Optional Accessories
for Liquid Samples
Cell Holders Used at Ambient Temperature
USE-753 Standard Rectangular Cell Holder
Sample: 1 Reference: 1
The USE-753 is the standard cell holder for the V-630/650/660/670 instruments. It accepts a
maximum of 10 mm x 10 mm cells to micro cells with a minimum optical path width of 2 mm
and optical path length of 10 mm, useful for measurement of very small amounts of sample.
FSE-702 4-Position Long Path Cell Changer
Sample: 4 Reference: 1
The FSE-702 4-position Long Path Cell holder is designed for sample measurement of low
absorption solutions using a long light path rectangular cell (max. path length of 100 mm);
the 4 position cell holder manually positioned for selection of the measuring cell. Rectangular
cells having a light path of 10, 20, 50 or 100 mm can be used with this accessory. A cell of
the same length can also be placed in the reference position.
Cell
Rectangular cell, 10 mm path width, 10, 20, 50 and
100 mm path length
Number of cells for sample side
Max. 4
Reference
Rectangular cell, 10 mm path width, 10, 20, 50 and
100 mm path length
Number of cells for reference side 1
LSE-701 Long Path Cell Holder
Sample: 1 Reference: 1
The LSE-701 Long Path Cell holder is designed for sample measurement of low absorption
solutions using a long light path rectangular cell (max. path length of 100 mm). Rectangular
cells having a light path of 10, 20, 50 or 100 mm can be used with this accessory. A cell of
the same length can also be placed in the reference position.
Cell
Rectangular cell, 10 mm path width, 10, 20, 50 and 100 mm path length
Reference
Rectangular cell, 10 mm path width, 10, 20, 50 and 100 mm path length
SSE-704 6-Position Manual Cell Holder
Sample: 6 Reference: 1
The SSE-704 6-position Manual Cell holder is designed to accept up to 6 cells for high
throughput measurements; the 6 position cell holder manually positioned for selection of the
measuring cell. Six standard cuvettes of 10 x 10 mm can be used with this accessory and a
cell can also be placed in the single reference position.
Cell
Standard cuvette: 10 x 10 mm
Number of cells for sample side
Max. 6
Reference
Standard cuvette: 10 x 10 mm
Number of cells for reference side 1
Optional Accessories for Liquid Samples continued
15
V-600 Series Optional Accessories
for Liquid Samples
Cell Holders Used at Ambient Temperature
CYH-708 Cyrindrical Cell Holder
Sample: 1 Reference: 1
The CYH-708 cylindrical cell holder is an accessory designed for sample measurement
using a cylindrical cell (Outer diameter: 22 or 30 mm). A cylindrical cell with a light path of
50 or 100 mm can be used.
Cell used
Cyrindrical cell, 50 mm or 100 mm Path length
Reference
Cyrindrical cell, 50 mm or 100 mm Path length
Ncp-705 6-Position Automatic Cell Changer
Sample: 6 Reference: 1
The 6-Position Automatic Cell Changer accessory provides the ability to automatically switch
between a maximum of 6 standard cuvettes of the 10 x 10 mm format. This accessory can
be used for measurement of multiple samples or for the simultaneous monitoring of multiple
kinetics reactions.
Cell used
Standard cuvette: 10 x 10 mm
Number of cells for sample side
Max. 6
Reference
Standard cuvette: 10 x 10 mm
Number of cells for reference side 1
Constant Temperature Cell Holders Used at Controlled
Temperatures
The following cell holder accessories can be used in conjunction with constant temperature
water recirculators for maintaining the samples at a uniform temperature. These accessories
are suitable for measurement of samples that are readily affected by changes in temperature.
The recirculator system is available separately.
STR-707 Water Thermostatted Cell Holder with Stirrer
Sample: 1 Reference: 1
The STR-707 Water Thermostatted Cell Holder with Stirrer is used with a commercially
available water circulating bath (not supplied) to keep the sample temperature constant by
circulating water around the outside of the sample cell.
The sample cell accepts a standard 10 x 10 mm cuvette. A stirrer magnet rotates to stir the
sample during observation.
16
Cell
Standard cuvette: 10 x 10 mm, to 10 x 2 mm light path length
Temperature control
Thermostatted water circulation
Operating temperature
10 to 90ºC
Reference
Standard cuvette: 10 x 10 mm
Stirring
Integrated variable speed magnetic stirrer
Mht-745 Manual 4-Position Water Thermostatted Turret Cell Holder
Sample: 4 Reference: 1
The MHT-745 is used with a commercially available water thermostatted circulating bath
(not supplied) to provide a constant sample temperature by circulation of water around the
outside of the sample cell.
The 4-position turret cell holder can be rotated manually and permits measurement of the
reaction processes of four different samples or changes in the sample spectra with time.
Cell
Standard cuvette: 10 x 10 mm to 2 mm path length, 4 pcs.
Temperature control
Thermostatted water circulation
Operating temperature
10 to 90ºC
Reference
Standard cuvette: 10 x 10 mm
HMC-711 Water Thermostatted Micro Cell Holder
Sample: 1 Reference: 1
The HMC-711 Water Thermostatted Micro Cell Holder is used with a commercially available
water circulating bath (not supplied) to keep the sample temperature constant by circulating
water around the outside of the sample cell. The compatible sample cell is either a 5 x 10
mm or 10 x 10 mm cell with a light path width of 2 to 4 mm.
Cell
10 x 10 mm square cuvette, 2 - 4 mm path width micro cell
(1 pc.)
or
5 x 10 mm cuvette, 2 - 4 mm path width micro cell (1 pc.)
Temperature control
Thermostatted water circulation
Operating temperature
10 to 90ºC
Reference
Rectangular cell, 10 mm path width, 10 mm light path, 1 pc.
Stirring
Integrated variable speed magnetic stirrer
Cell Masks (standard)
Mask for 0.1 mL cell (2 pcs.) (for Rectangular cell, 5 mm
path length, 2 mm path width)
Mask for 0.2 mL cell (2 pcs.) (for Rectangular cell, 10 mm
path length, 2 mm path width)
Ncp-706 Water Thermostatted 6-Position Automatic Cell Changer
Sample: 6 Reference: 1
The NCP-706 accessory automatically moves up to six cells into the light path of the
spectrophotometer for measurement. In the auto-measurement mode, each cell is
automatically measured in the specified order, starting with cell 1; alternatively, a specific cell
can be selected for a manual measurement of the sample cell. The NCP-706 maintains the
sample at a constant temperature by circulating water using a thermostatted recirculating
water bath (not supplied).
Cell used
Standard 10 x 10 mm cuvette, 10 mm to 2 mm (micro) path
width
Number of cells for sample
side
Max. 6
Temperature control
Thermostatted water circulation
Operating temperature
10 to 90ºC
Reference
Rectangular cell, 10 mm path width, 10 mm path length
Number of cells for
reference side
1
Stirring
Integrated variable speed magnetic stirrer
Optional Accessories for Liquid Samples continued
17
V-600 Series Optional Accessories
for Liquid Samples
Peltier Thermostatted Cell Holders
Optional Sample Lid for STR-707, EHC-716, ETC-717 Accessories
CSP-748 Lid with Syringe Port for V-650/660/670
CSP-749 Lid with Syringe Port for V-630
The syringe port lid accessory is used for adding a reagent enzyme or other reactant solution
with a syringe and monitoring the change in Abs after injection of the reagent solution.
When monitoring a substrate-enzyme reaction, this accessory allows addition of an enzyme
solution without opening the sample chamber lid. This lid can be utilized with the model
STR-707 (Constant Temperature Cell Holder with Stirrer) or other single-cell accessories.
A syringe with a needle length of 5 cm is recommended.
Recommended syringe:
0507-0220 Micro syringe, 10 µL
0507-0246 Micro syringe, 100 µL
Ehc-716 Peltier Thermostatted Single Cell Holder(Air-Cooled)
Etc-717 Peltier Thermostatted Single Cell Holder (Water-Cooled)
Sample: 1 Reference: 1
EHC-716
This single-cell sample holder accessory utilizes the Peltier effect to precisely maintain the
sample temperature. Temperature control using a Peltier-effect controller under PC control
ensures stable temperature even when the set temperature is in the room temperature range.
The cell holder is equipped with a stirrer, enabling uniform cell temperature control. The
EHC-716 cell holder is air cooled, and the ETC-717 cell holder is water cooled for a greater
temperature control range.
EHC-716
ETC-717
18
ETC-717
Cell
Standard 10 x 10 mm cuvette (sample and reference)
Temperature control system
Heating/cooling system utilizing Peltier effect
Peltier heat radiation
Air cooled
Stirring system
Magnetic stirrer with variable speed
Temperature setting range
5 to 70ºC
-10 to 110ºC
Temperature control range
10 to 60ºC (for room temperature =
20ºC)
0 to 100ºC (for room
temperature = 20ºC)
Temperature control
accuracy
± 0.1ºC
Temperature accuracy
(Difference between displayed temperature and actual cell
temperature)
20ºC to 40ºC ±0.5ºC
±1ºC outside the above range
Temperature setting
difference
(Difference between set temperature and displayed
temperature)
20ºC to 40ºC ±0.3ºC
±0.5ºC outside the above range
Water cooled
Optional for Etc-716, ETC-717
Ops-512/514 Optional Temperature Sensor
The OPS-512/514 OP temperature sensor is an optional accessory used to directly measure
solution temperature in the cell through direct contact when used with the ETC-716 or EHC717. Unlike typical typical temperature measurement performed using the cell holder sensor,
the OPS-512/514 is dipped directly into the solution to measure the sample temperature as
required.
The OPS-512 is used with a 10 mm rectangular cell.
The OPS-514 is a narrow type that can also be used with micro cells.
Optional Mask Kit for 100 µL Micro Cell
This is a cuvette ‘mask’ kit for the EHC-716 and ETC-717. It comprises sample masks and
a ‘shim’ to raise the cuvette height. Used in conjunction with micro cells with an optical
path length of 10 mm and optical path width as small as 2 mm, it enables measurement of
samples with a minimum 100 μl volume.
Psc-718 Automatic 6-Position Peltier Cell Changer (Air-Cooled)
Sample: 6 Reference: 1
The Model PSC-718 accessory can be used to automatically measure up to six samples
using the V-600 series spectrophotometers. This cell holder utilizes the Peltier effect to keep
the sample temperature constant with high accuracy. Equipped with a magnetic stirrer, the
PSC-718 accessory provides precise, stable control of the temperature in all 6 sample cells.
Cell
Standard 10 x 10 mm cuvette
Temperature control system
Heating/cooling system utilizing Peltier effect
Peltier heat radiation
Forced air cooling
Stirring system
Integrated variable speed magnetic stirrer
Temperature setting range
10 to 70ºC
Temperature control range
15 to 60ºC (when room temperature is 20ºC)
Temperature control accuracy
±0.1ºC
Temperature accuracy
(Difference between the
displayed temperature and
temperature in the cell)
20ºC to 40ºC ±0.5ºC
±1ºC outside the above range
Temperature setting difference
(Difference between the
displayed temperature and
temperature in the cell)
20ºC to 40ºC ±0.3ºC
±0.5ºC outside the above range
Optional OPS-513 In-cell sensor
This optional sensor can be used to monitor the temperature inside one of the sample cells.
Data processing can then be performed with the temperature data obtained from the in-cell
sensor.
Optional Accessories for Liquid Samples continued
19
V-600 Series Optional Accessories
for Liquid Samples
Peltier Thermostatted Cell Holders
PWC-758 Automatic 8x8-Position Peltier Cell Changer (Water-Cooled)
Sample: 8 Reference: 8
The PWC-758 cell holder accessory can automatically be used to measure up to eight
samples and corresponding reference cells using the V-600 series spectrophotometers. The
accessory employs the Peltier effect to precisely heat or cool the samples using PC control
of the Peltier element, even when the set temperature is near room temperature. A magnetic
stirrer maintains a uniform cell temperature for all eight sample cell positions.
Available for V-650/660/670 instruments only, Spectra Manager PC control only.
Cell
Sixteen standard 10 x 10 mm cuvettes (Sample,
Reference side)
Temperature control system
Heating/cooling system using Peltier effect
Peltier heat radiation
Water cooled
Stirring system
Integrated variable speed magnetic stirrer
Temperature setting range
-10 to 110ºC
Temperature control range
0 to 100ºC (when room temperature is 20ºC)
Temperature setting precision
±0.3ºC
Temperature accuracy
(Difference between displayed
and actual cell temperature)
20ºC to 40ºC ±0.5ºC
±1ºC outside the above range
Optional In-Cell Sensor
This optional sensor allows monitoring of the sample cell temperatures, up to eight sensors
can be attached to monitor all eight sample cells. Up to eight sensors can be used to provide
precise temperature data for all eight sample cells.
Options for PAC 743 and PAC-743R
Rectangular Quartz Cell
6-Position
Cell Changer
2
8-Position
Micro 8-Position
Rectangular Quartz Micro Cell
5mm Rectangular Quartz Cell
Micro 8-Position Cell
4
5
Sensor in the Cell
For current part number, reference www.jascoinc.com/V600part#.
20
3
Rectangular Quartz Micro
Black Cell
PAC-743
PAC-743R
1
6
7
Pac-743 Automatic 6/8-Position Peltier Cell Changer (Water-Cooled)
Pac-743r Automatic 6/8-Position Peltier Cell Changer (Water-Cooled,
Thermostatted Reference)
Sample: 6/8 Reference: 1
The PAC-743/PAC-743R Automatic 6/8-Position Peltier Cell Changers
allow measurements of the transmittance/absorbance of multiple sample
cells by using dedicated cell blocks. With a temperature range from 0 to
100ºC, three dedicated cell blocks are available, an 8-position micro cell
block (P/N 6916-H443A), an 8-position 5 mm cell block (P/N 6916-H343A)
and a 6-position 10 mm cell block (P/N 6916-H343A). Eight samples at a
time from a 96 microwell microtiter plate (8x12) can be directly transferred
to the 8-position micro cell block with an 8-position micro pipette. The
PAC-743R accessory provides thermal control of the reference cell in
addition to temperature control of the sample cells.
Cell:
PAC-743 equipped with
8-position cell block and
8-position micro cell
Sample side: Optional Cell Block
Reference side: Standard 10 x 10 mm cuvette
Stirring system
Integrated variable speed magnetic stirrer
Temperature control system
Heating/cooling system utilizing Peltier effect
Control of the temperature of sample and reference side
(PAC-743: sample side only)
Peltier heat radiation
Water cooled
Temperature setting range
-10 - 110ºC
Temperature control range
0 to 100ºC (for room temperature = 20ºC)
Temperature control accuracy
+/-0.1ºC
Temperature accuracy
+/- 0.5ºC (20 to 40ºC), +/- 1ºC (Difference between
displayed and actual cell block temperature)
Temperature monitoring
power output
0 to 100ºC, 10mV/ºC
Optional Accessories for Liquid Samples continued
1
6-position cell block
This cell block holds six cells with an optical path length of 10 mm (10 mm to 2 mm
light path width). An integrated stirrer assures optimum temperature stability within all
6 sample cells.
Optional temperature sensor available to directly monitor sample cell temperatures. Up to
six sensors can be attached for monitoring of all 6 sample cells.
2
8-position cell block
This cell block holds eight square cells with an optical path length of 5 mm (5 mm light path
width). An optional temperature sensor is available for monitoring of the individual sample
cells. Up to eight sensors can be attached for monitoring of all eight sample cells.
3
8-position micro-cell block
This cell block allows the attachment of a micro 8-position cell with an optical path length
of 10 mm and capacity of 100 µl per cell. The cell spacing is exactly the same as an
8-position micro-pipette for rapid sample transfer from a 96-well microplate.
4 Standard cuvette, light
path length: 10 mm, light
path width: 10 mm
5 Standard cuvette, light
path length: 10 mm, light
path width: 2 mm
6 Square cell, light path
length: 5 mm, light path
width: 5 mm
7 8-position micro cell, cell
volume: 100 µL
21
V-600 Series Optional Accessories
for Liquid Samples
Micro Cell Holders
TCH-703 8-Position Micro Turret Cell Holder for V-630
P/N : 6916-J003A
The TCH-703 is a cell holder for 8-position turret micro cells, containing eight cells with a
volume of approximately 4 μl arranged in a circle, the cell positions exchanged manually
using an external manipulator. This accessory is useful for measuring very small quantities of
multiple samples. Source energy is focused onto the micro-cell volume using a lens to obtain
highly accurate measurements.
Cell used
Sample side
8-position Turret Micro Cell, 1 mm path
length, Cell capacity: 4 µL (Must be
specified in addition to TCH-703 accessory)
Reference side
10 mm path length rectangular cell
Measurement repeatability (Coefficient of
variation)
5%
4 µL 8-Position Turret Micro Cell
P/N : 6916-4822B
Optional µWash
A cleaning system for micro cells.
The µWash cleaning system is a device specifically designed for cleaning micro cells/
cuvettes. A double barrel syringe allows the extraction of the used sample from a tiny micro
cell/cuvette, and flushing/washing of the cell volume with cleaning solvent by pressing the
flushing piston.
MW-1000 µWash, 1 mL type
The MW-1000 µWash is designed for use with micro cells/cuvettes whose cell volume is 1
mL to 100 µL. All parts of the MW-1000 µWash are included in single stand: flush and waste
syringes, a flush solvent bottle, and a waste solvent bottle.
Draining, Suction
Maximum 1 ml
Resistance to solvent
Water, Alcohols
Materials in contact with solvent
SUS, PE, PP, PTFE, EPDM, Silicon
MW-2000 µWash, 100 µL type
The MW-2000 is ideal when only a small amount of flush solvent (<100µL) is required. Flush
and waste syringes, a flush solvent tube, and waste solvent tubes (10mL) are all integrated in
a convenient, compact and portable holder.
22
Draining, Suction
Maximum 0.1 ml
Resistance to solvent
Water, Alcohols
Materials in contact with solvent
SUS, PE, PP, PTFE, EPDM, Silicon
EMC-709 Micro Cell Holder
P/N : 6916-J009A
The EMC-709 accessory provides the capability to perform spectral measurements with
50 µl micro cells. A 5 µl micro cell spacer (option) provides the capability to use 5 µl micro
cells. This cell holder should be specified for use of 5 µl micro cells with the V-650/660/670
instruments.
Optional spacer for 5 µL cell
Mask (2 x 1.5 mm)
Cell-height adjustment ring
EMC-759 5µL Ultra-Micro Cell Holder for V-630
P/N : 6916-J059A
The EMC-759 is a micro cell holder for a micro volume sample (about 5µl). This accessory
accepts a commercial rectangular micro cell with a light path length of 1 mm and a light
path width of 2 mm. By using lenses to focus the light beam onto the cell, highly precise
measurement is possible.
Lenses for focusing of source energy
Cell-height adjustment ring
Optional Micro Cells and Spacer for EMC-709 and EMC-759
50 µL Micro cell
Light path length: 10 mm
Light path width: 2 mm
Sample Volume: 50 µL
P/N: 1103-0114A
5 µL Micro cell
Light path length: 1 mm
Light path width: 2 mm
Sample Volume: 5 µL
P/N: 1103-0194A
5 µL Micro cell (Stepped
type)
Light path length: 1 mm
Light path width: 2 mm
Sample Volume: 5 µL
P/N: 1103-0201A
Spacer for 5 µL Micro cell
P/N: 6708-J046A
5 µL Micro Cell (Stepped Type)
The cell is designed so that a pipette tip can
reach to the cell bottom and sample recovery
can be easily performed.
Optional Accessories for Liquid Samples continued
23
V-600 Series Optional Accessories
for Liquid Samples
Flow System, Flow Cell, Sipper, Autosampler
Autosampler System With Microtiter Plates
Up to 192 samples can be automatically measured using 2 microtiter plates.
ASU-605
NCS-609
SFC-712
SFC-712 Flow Cell Holder for 5/10 mm Pathlength
P/N: 6916-J012A
The SFC-712 can be used for measuring temporal changes of a continuously pumped
sample, or, in conjunction with an automatic sampling accessory (ASU-605) and a syringe
pump. Two different flow cell blocks can be specified (option) with different optical path
lengths of 5 or 10 mm.
Optional Flow Cell Blocks
5 mm pathlength flow cell block (P/N : 6516-H607A)
10 mm pathlength flow cell block (P/N : 6516-H608A)
24
Flow cell material
Fused quartz
Light path length
10 mm
5 mm
Cell capacity
100 µl
50 µl
LFC-713 Long Path Flow Cell Holder
P/N: 6916-J013A
The LFC-713 can be used for measuring temporal changes of a continuously pumped
sample, or, in conjunction with an automatic sampling accessory (ASU-605) and a syringe
pump. Three different flow cell blocks can be specified (option) with different optical path
lengths of 30, 50 or 100 mm. The long optical path lengths of the optional flow cell blocks
are extremely useful for samples of very low absorbance.
Optional Flow Cell Blocks
30 mm pathlength flow cell block (P/N : 6522-J343A)
50 mm pathlength flow cell block (P/N : 6522-J333A)
100 mm pathlength flow cell block (P/N : 6522-J243A)
Flow cell material
Fused quartz
Light path length
30 mm/50 mm /100 mm
Cell capacity
30 mm path: approx. 0.6 ml
50 mm path: approx. 1 ml
100 mm path: approx. 2 ml
ASU-605 Autosampler Unit
P/N: 6774-J001A
The ASU-605 is an autosampler for automated analysis
of samples in test tubes, using a special test tube
rack. A nozzle moves in the X (left-right), Y (backwardforward), and Z (up-down) directions to withdraw
a sample automatically from the test tubes using a
peristaltic, vacuum pump or syringe pump. The ASU605 can also be used for auto-sampling of 2, 96-well
microtiter plates.
Optional Racks for 15mm-dia. test tubes
(15OD x 105 mm, 12 mL) (maximum 100 samples)
Optional Racks for 12mm-dia. test tubes
(12OD x 75 mm, 5 mL) (maximum 150 samples)
Optional Racks for 10mm-dia. test tubes
(10OD x 75 mm, 3 mL) (maximum 150 samples)
Optional Racks for two 96-well microplates
(1 mL) (maximum 192 samples)
Optional Thermostatted rack for two 96-well microplates
(1 mL) (maximum 192 samples)
Optional DPC-619 Dust Cover
Optional AWU-620 Washing Unit
This is a washing unit specifically for the NQF-720 vacuum sipper and NPF-721 Peristaltic
sipper. This optional unit can be loaded with a solvent for automatic washing of the ASU-605
flow tubing to prevent sample carryover.
Nozzle
Stainless nozzle standard
Tubing
Teflon (2.0 mm (OD), 1.0 mm (ID))
Applicable pump systems
NQF-720 Vacuum sipper
NPF-721 Peristaltic sipper
NCS-609 Syringe pump
Optional Accessories for Liquid Samples continued
25
V-600 Series Optional Accessories
for Liquid Samples
Flow System, Flow Cell, Sipper, Autosampler
NPF-721 Peristaltic Sipper (with flow cell unit)
P/N: 6916-J021A
The Model NPF-721 peristaltic sipper is an accessory designed for use with the JASCO
UV-Vis/NIR spectrophotometers for rapid measurements of multiple samples. The unit can
be operated simply by inserting the sample nozzle into a vessel filled with sample solution
and pressing the peristaltic pump lever. The average amount of sample required is 1.5 to 2.0
ml. Sample can be recovered by reversing the pump direction. The accessory design allows
simple switching between using the integrated 10 mm flow cell or a 10 mm rectangular cell.
Light path length
10 mm
Cell capacity
Approx. 50 µl
Cell material
Quartz
Carryover
less than 1%
Minimum sample requirement
0.7 mL with low-viscosity samples
Tubing materials
Teflon, Tygon, PharMed tubing
Throughput
360 samples/hr.
NQF-720 Vacuum Sipper (with flow
cell unit)
P/N: 6916-J020A
This sipper can be integrated with the
V-600 series instruments to speed the
measurement of multiple samples. Simply
insert the nozzle into a container filled with
sample solution and press the sample fill
lever. This accessory can process up to
450 samples per hour. Teflon and similar
materials are used in the tubing for superior
chemical resistance. The accessory design
allows simple switching between using
the integrated 10 mm flow cell or a 10 mm
rectangular cell. The cell block can also
be replaced with the optional LPY-611
long optical path flow cell block to obtain
measurements using a flow cell with an
optical path length of 50 mm.
26
Optional LPY-611 Long Path Cell Block Unit
P/N: 6708-J053A
50 mm pathlength flow cell block
With standard cell block
With LPY-611
Light path length
10 mm
50 mm
Cell capacity
Approx. 50 µl
Approx. 980 µl
Cell material
Quartz
Quartz
Carryover
less than 1% with low-viscosity samples
Minimum sample
requirement
0.7 mL
Tubing materials
Teflon, Viton
2.4 mL
NCS-609 Syringe Pump
P/N: 6708-J051A
The NCS-609 syringe pump can be used in conjunction with the SFC-712 flow cell holder
in the automatic sample system. The syringe pump is suitable for drawing small quantities
of sample, and essential for measurement of samples using microtiter plates. For superior
chemical resistance, parts in contact with liquids are composed of PEEK, teflon and glass.
Reproducibility of volume delivery:
within ±1 %
Syringe capacity:
2.5 mL
Optional Accessories for Liquid Samples continued
27
V-600 Series Optional Accessories
for Liquid Samples
Optional Cell Holders
Single Cell Accessories
General 10 X 10 mm
Rectangular Cells
At Ambient Temperature
Temperature Control by
Water Circulation
Temperature Control by
Peltier Effect
Path length:
10 mm
Path width:
10 mm
USE-753 Rectangular Cell Holder STR-707 Constant Temperature
Cell Holder with Stirrer
EHC-716/ETC-717 Peltier
Thermostatted Single Cell Holder
More than 1 mL
Path length:
10 mm
Path width:
4 mm
USE-753 Rectangular Cell Holder HMC-711 Constant temperature
rectangular micro cell holder
EHC-716/ETC-717 Peltier
Thermostatted Single Cell Holder
More than 400 µL
Path length:
10 mm
Path width:
2 mm
USE-753 Rectangular Cell Holder HMC-711 Constant temperature
rectangular micro cell holder
EHC-716/ETC-717 Peltier
Thermostatted Single Cell Holder
Optional mask with micro cell
required
For Small Volume
Micro Cells
More than 400 µL
Path length:
10 mm
Path width:
2 mm
USE-753 Rectangular Cell Holder
Cell jacket and mask are required.
More than 50 µL
Path length:
5 mm
Path width:
2 mm
HMC-711 Constant temperature
rectangular micro cell holder
More than 50 µL
Path length:
10 mm
Path width:
2 mm
EMC-709 Micro Cell Holder
More than 5 µL
Path length:
1 mm
Path width:
2 mm
EMC-759 Micro Cell Holder
For Low Absorption Long
Pathlength Rectangular Cell
LSE-701 Long Path Cell holder
10, 20, 50 and 100 mm
rectangular cells
CYH-708 Cylindrical Cell Holder
Up to 100 mm path length,
cylindrical cells, with 22 or 30 mm
dia.
See page 20 - 21 for Various Options
TCH-703
8-position Turret 4 µL
Cell Holder
V-630 Only
28
5 x 5 mm
8-position
100 µL cell
UV/VIS spectroscopic analysis is a common analytical procedure in which the liquid sample spectrum is measured and/
or quantitative analysis can be performed using the maximum absorption wavelength. Today, increasingly sophisticated
applications that include time and temperature variables are being employed in such fields as biochemistry and
biotechnology, and in clinical laboratories. JASCO offers many kinds of sampling accessories to support the growing
demand for complex applications and provides the ideal system tailored to meet specific customer requirements. In a
PC controlled configuration, these intelligent accessories enable high-speed data acquisition and high measurement
accuracy. The tables below apply to all instruments of the V-600 series, except where otherwise noted.
Multi Cell Accessories
General 10 X 10 mm
Rectangular Cells
At Ambient Temperature
Temperature Control by
Water Circulation
Temperature Control by
Peltier Effect
Path length:
10 mm
Path width:
10 mm
NCP-705 6-position Automatic
Cell Changer
PC Controlled instruments only
NCP-706 Constant-temperature PSC-718 6-position Automatic
6-position Automatic Cell Changer Peltier Cell Changer
PC Controlled instruments only
PC Controlled instruments only
SSE-704 6-position Manual Cell
Holder
MHT-745 4-position Constant
Temperature Turret Cell Holder
PWC-758 8-position Automatic
Peltier Cell Changer
PC Controlled instruments only
PAC-743 6/8-position Automatic
Peltier Cell Changer
Optional cell block is required
PC Controlled instruments only
NCP-706 Constant-temperature PSC-718 6-position Automatic
6-position Automatic Cell Changer Peltier Cell Changer
PC Controlled instruments only
PC Controlled instruments only
More than 1 mL
Path length:
10 mm
Path width:
4 mm
MHT-745 4-position Constant
Temperature Turret Cell Holder
PWC-758 8-position Automatic
Peltier Cell Changer
PC Controlled instruments only
(Not applicable to the V-630/V-630
Bio)
PAC-743 6/8-position Automatic
Peltier Cell Changer
Optional cell block is required
PC controlled instruments only
More than 400 µL
Path length:
10 mm
Path width:
2 mm
NCP-706 Constant-temperature PSC-718 6-position Automatic
6-position Automatic Cell Changer Peltier Cell Changer
PC Controlled instruments only
PC Controlled instruments only
MHT-745 4-position Constant
Temperature Turret Cell Holder
PWC-758 8-position Automatic
Peltier Cell Changer
PC Controlled instruments only
PAC-743 6/8-position Automatic
Peltier Cell Changer
Optional cell block is required
PC Controlled instruments only
For Small Volume
Micro Cells
More than 400 µL
Path length:
10 mm
Path width:
2 mm
PAC-743 6/8-position Automatic
Peltier Cell Changer
Optional cell block is required
PC Controlled instruments only
More than 50 µL
Path length:
5 mm
Path width:
2 mm
PAC-743 6/8-position Automatic
Peltier Cell Changer
Optional cell block is required
PC Controlled instruments only
For Low Absorption Long
Pathlength Rectangular Cell
FSE-702 4-Position Long Path
Cell Holder
10, 20, 50 and 100 mm
rectangular cells
29
V-600 Series Optional Accessories
for Solid Samples
To meet the ever increasing demand for
evaluation of manufactured materials
such as polymers, glass, ceramics and
semiconductors, several different kinds
of accessories are required. Many of
the varying properties of all types of
solid samples require not only UV-Vis
measurement, but also measurement into
the NIR region. Moreover, multivariate
analysis in the NIR region may be necessary
to determine ingredients of various samples
such as food products. To satisfy these
requirements, JASCO offers a wide range of
accessories for solid samples.
The V-600 Series covers a wide range
of solid sample applications such as
materials analysis, film thickness, optical
property evaluation of optical elements and
quality control of high technology devices,
employing optional accessories and
dedicated software programs.
Smooth Surface
Transparent Sample
Regular Transmittance
Film holders
FLH-740 Film holder (leaf spring type)
FLH-741 Film holder (sliding mount type)
RSH-744 Rotary sample holder
VTA-752 Film holder (Variable incident angle)
Reflectance Sample
Relative specular reflectance using
a standard sample mirror
Specular Reflectance Accessories
SLM-736/737 Specular reflectance accessory
SLM-738Specular reflectance accessory
for 6-inch wafers
Absolute specular reflectance without
a standard sample mirror
Concave
mirror
Incident
light
100% line
Sample
Measurement
Sample
Integrating
sphere
30
Absolute Reflectance Accessories
ARV-730/731Absolute reflectance
accessory (Synchronous)
ARSV-732/733Absolute reflectance
accessory(Asynchronous)
ARMV-734/735Automated absolute
reflectance accessory
Diffuse Transmittance and Reflectance Samples
Transparent Sample
Diffuse Transmittance
Integrating Spheres
ISV-72260mm integrating sphere (UV/VIS)
ISN-72360mm Integrating sphere (UV/VIS/NIR)
ILV-724150mm integrating sphere (UV/VIS)
ILN-725150mm integrating sphere (UV/VIS/NIR)
IJV-726Dedicated gemstone integrating sphere (UV/VIS)
IJN-727Dedicated gemstone integrating sphere (UV/VIS/NIR)
Diffuse Reflectance
HISV-728Portable integrating sphere (UV/VIS)
HISN-729Portable integrating sphere (UV/VIS/NIR)
PIV-756Horizontal sampling integrating sphere (UV/VIS)
PIN-757Horizontal sampling integrating sphere (UV/VIS/NIR)
Film Holders
FLH-740 Film Holder (Leaf Spring Type)
P/N: 6916-J040A
FLH-741 Film Holder (Sliding Mount Type)
P/N: 6916-J041A
These film holder accessories are used for
measuring the transmittance of solid samples
such as films, plate glass, and filters. An
accessory can be selected to match the thickness
and physical size of the samples.
flh-740
FLH-740 Film Holder
Thickness of sample
0.5 to 10 mm
Sample size
Maximum 80 x 100 mm
Minimum 15 x 15 mm
flh-741
FLH-741 Film Holder
Thickness of sample
0.5 to 25 mm
Sample size
Maximum 80 x 100 mm
Minimum 5 x 5 mm
RSH-744 Rotary Sample Holder
P/N: 6916-J044A
The RSH-744 can be used to rotate the sample
manually, both around the optical axis (in-plane
rotation of the sample) and by the tilt angle, for
measuring the transmittance of a film sample.
Sample size
Minimum 10 x 30 mm
Maximum 18 x 38 mm
Sample thickness
1 to 2 mm
Reference beam side
Film holder
Sample holder rotation range
Angle of rotation around optical axis: 360º
Angle of rotation around the horizontal axis
perpendicular to the optical axis: ± 50º
Optional Accessories for Solid Samples continued
31
V-600 Series Optional Accessories
for Solid Samples
Film Holders
VTA-752 Film Holder (Variable
Incident Angle)
P/N: 6916-J052A
The VTA-752 Film Holder is an accessory
to measure transmittance of a film type
sample, changing the incident angle of the
light beam. The incident angle of the light
beam can be set in 1º intervals.
Sample size
Min. 15 mm (H) x 35 mm (W) x 1 mm (T)
Max. 80 mm (H) x 70 mm (W) x 2 mm (T)
Reference
Film holder
Sample holder rotation range
±90º
Optional High Absorbance
Measurement Kit
ND Filter
32
Shutter Plate
ND Filter (OD 2) for
liquid samples
This kit enables accurate measurement of
highly absorbing samples with baseline
correction using attenuation of the reference
beam. The kit consists of a Neutral Density
(ND) filter and a shutter plate for solid
samples. An ND filter (OD 2) is used for
liquid sample measurements.
Specular Reflectance Accessory
SLM-736 Specular Reflectance Accessory for V-650, V-660, V-670
P/N: 6916-J036A
SLM-737 Specular Reflectance Accessory for V-630
P/N: 6916-J037A
SLM-738 Specular Reflectance Accessory (Accommodating 6-inch Silicon
Wafers) for V-650, V-660, V-670
P/N: 6916-J038A
The SLM-736/737 Specular reflectance accessories are designed to measure the relative
reflectance of a sample using the reflected light from an aluminum-deposited plane mirror
as a reference. This unit permits measurement of the reflectance of metal-deposited films,
metal plating, etc., as well as measurement of film thickness using a film thickness analysis
program. The SLM-738 can measure larger samples such as 6 inch silicon wafers.
SLM-736
SLM-737
SLM-738
Main units
V-650/660/670
V-630
V-650/660/670 (accommodating
6-inch wafers)
Angle of Incidence
Approx. 5º fixed
Approx. 5º fixed
Approx. 5º fixed
Minimum Sample Size
10 x 10 mm
10 x 10 mm
-
Maximum Sample Size
100 x 120 mm
100 x 120 mm
150 mm diameter
Beam Port Diameter
7 mm (4 mm, 2 mm as
option)
7 mm (4 mm, 2 mm
as option)
7 x 7 mm
Reflection Reference
Aluminum-deposited plane mirror (Standard mirror)
Wavelength Range
200 to 870 nm (V-650/660) 250 to 1000 nm
200 to 2700 nm (V-670)
SLM-736/737
200 to 870 nm (V-650/660)
200 to 2700 nm (V-670)
SLM-738
Optional Sample Chamber Lid for SLM-736
P/N: 6916-H236A
Optional Sample Chamber Lid for SLM-737
P/N: 6916-H137A
Optional Sample Stage with Mask for SLM-736/737
This is a sample stage combined with a mask for the SLM-736/737. It
is used for measuring small samples that cannot be measured on the
standard sample stage.
MSK-001 Sample stage with 2-mm-dia. mask (P/N: 6708-J103A)
Sample size: Minimum 3x3 mm, Maximum 50x50 mm
MSK-002 Sample stage with 4-mm-dia. mask (P/N: 6708-J203A)
Sample size: Minimum 5x5 mm, Maximum 50x50 mm
MSK-001
MSK-002
Optional Accessories for Solid Samples continued
33
V-600 Series Optional Accessories
for Solid Samples
Specular Reflectance Accessory
Optional GHP-506 Polarizer
P/N: 6708-J026A
Spectral Range: 215 - 2300 nm
Optional DPL-515 Depolarization Plate
P/N: 6708-J068A
Spectral Range: 350 - 2500 nm
The light from a spectrophotometer employing a grating always exhibits some polarization
characteristics. The ratio of the intensities of the S polarized light and P polarized light can
vary according to the wavelength and also differs from one grating to another for the same
wavelength. The greater the angle of incidence of the beam on the sample, the greater the
difference in the intensity of the S polarized reflected light and the P polarized reflected light,
making measurements inaccurate if the absolute reflectance is measured at a high angle
of incidence. In such a case, a polarizer is required to accurately measure the absolute
reflectance when the angle of incidence is 30° or greater.
34
Integrating Spheres
ISV-722 60 mm Integrating Sphere for UV-Vis for V-650 and V-660
P/N: 6916-J022A
ISN-723 60 mm Integrating Sphere for UV-Vis/NIR for V-670
P/N: 6916-J023A
ILV-724 150mm Integrating Sphere for UV-Vis for V-650 and V-660
P/N: 6916-J024A
ILN-725 150mm Integrating Sphere for UV-Vis/NIR for V-670
P/N: 6916-J025A
The integrating spheres are designed to measure either the diffuse transmittance or diffuse
reflectance of a sample. Normally, the UV-Vis or UV-Vis/NIR spectrophotometers measure
the transmittance of a homogeneous, transparent liquid or solid sample. However, when a
turbid liquid sample or opaque solid sample is measured, the light incident upon the sample
is diffusely transmitted or reflected and only a small portion of the light reaches the detector,
resulting in an inaccurate measurement. The integrating sphere accessory acquires most of
the light diffuse-transmitted or diffuse-reflected from the sample into the integrating sphere
and introduces it to the detector, permitting more representative measurement of the sample.
Integrating spheres are provided with a light trap so that the reflectance of samples can
be measured with or without the specular reflectance component. For measurement of
the diffuse transmission of solid samples, the optional solid sample holder is required. For
measurement of the diffuse reflectance of powders, the optional powder sample holder
is required. For measuring liquids with the ISV-722/ISN-723 integrating sphere unit, the
reference side cell holder is an option.
60 mm Integrating Sphere
150 mm Integrating Sphere
Model name
ISV-722
ISN-723
ILV-724
ILN-725
Inside diameter
of integrating sphere
60 mm
60 mm
150 mm
150 mm
20 (H) x 20 (W) x 0.5 (T) mm
Minimum sample size
(Reflectance measurement)
20 (H) x 20 (W) x 0.5 (T) mm
65 (H) x 50 (W) x 25 (T) mm
Maximum sample size
(Reflectance measurement)
100 (H) x 50 (W) x 30 (T) mm
Sample cell
(Transmittance
measurement)
Light path length 5, 10, 20, 30, 50
mm rectangular cell
(sample side)
Light path length 5, 10, 20,
30, 50 mm rectangular cell
(sample side)
Light path length 5, 10, 20 mm
rectangular cell
(reference cell) (Cell block is
optional.)
Light path length 5, 10, 20,
30, 50 mm rectangular cell
(reference cell)
Wavelength range
200 - 870 nm
220 - 850 nm 220 - 2200 nm
Incident angle to
reflection surface
0º, Approx. 5º
0º, Approx. 5º
Approx. 5º
(with specular
(with specular
reflectance trap) reflectance trap)
200 - 2500 mm
Integrating spheres
cannot be used with
the V-630 instrument,
but can only be used
with the V-650/660/670
instruments.
Approx. 5º
Optional Accessories for Solid Samples continued
35
V-600 Series Optional Accessories
for Solid Samples
Integrating Spheres
Optional PSH-001 Powder Sample Holder
P/N: 6708-J305A
This accessory is used to measure the diffuse reflectance of a powdered sample.
Size of sample area: 16 mm diameter
Thickness: 0.5 - 6 mm
Optional SSH-506/507 Solid Sample Holder
P/N: 6708-J404A
This sample holder is used to measure the diffuse transmittance of a solid sample. SSH-506
is used with ISV-722/ISN-723. SSH-507 is used with ILV-724/ILN-725.
Model name
SSH-506
SSH-507
Minimum sample size
20 (H) x 20 (W) x 0.5 (T) mm
20 (H) x 20 (W) x 0.5 (T) mm
Maximum sample size
70 (H) x 40 (W) x 35 (T) mm
70 (H) x 30 (W) x 40 (T) mm
Optional Model RLH-603 Reference-Side Rectangular Cell Holder
P/N: 6708-J306A
This cell holder is required for the reference side for measurement of diffuse transmittance of
liquid samples when using the ISV-722/ISN-723 accessories.
Cell to be used: 5, 10, 20 mm optical pathlength rectangular cell
PIV-756 Horizontal Sampling Integrating Sphere for UV-Vis
P/N: 6916-J056A
PIN-757 Horizontal Sampling Integrating Sphere for UV-Vis/NIR
P/N: 6916-J057A
Integrating spheres for horizontal sampling, models PIV-756/PIN-757,
can mount the sample horizontally and provide measurements of small
sample sizes by using a lens.
36
Model name
PIV-756
PIN-757
Inside diameter of integrating
sphere
60 mm
60 mm
Minimum sample size
(Transmittance measurement)
3 (dia.) x 0.5 (T) mm
3 (dia.) x 0.5 (T) mm
Maximum sample size
(Transmittance measurement)
50 (H) x 50 (W) x 2 (T) mm
50 (H) x 50 (W) x 2 (T) mm
Maximum sample size
(Reflectance measurement)
30 (H) x 30 (W) x 10 (T) mm 30 (H) x 30 (W) x 10 (T) mm
Reflectance measurement
adaptor
20 (dia.) x 2 mm without window plate
Wavelength range
200-870 nm
200-2500 nm
Optional Lens and Mask for Reflectance Measurement
Optional Lens and Mask for Transmittance Measurement
Three mask sizes are available with diameters of 1, 2 and 3 mm. When the 1 mm diameter
mask is used, the beam diameter of the incident light upon the sample is decreased to a
minimum of 2 mm in diameter.
For diffuse transmittance measurements, the sample
is simply placed horizontally over the opening of the
integrating sphere and the incident light can be targeted
upon the sample simply by viewing the light incident
upon the sample. The sample is not fixed with springs or
clips, so this option is suitable for samples that are easily
damaged such as thin films on transparent substrates.
Samples with a wide variety of shapes can be measured
such as solid samples with peculiar shapes, for example,
small resin pellets or crystals or liquid films between
sheets of glass which are normally difficult to handle.
In addition, collection lenses which focus the incident beam
to a minimum of 2 mm in diameter are also available as an
option. Lenses are available for transmission or reflection
measurements and either type consist of a collection lens
and mask.
A sample set on the PIV-756 for diffuse
transmittance measurement.
Sample holder for reflection
measurement.
For transmission measurements, the position of the sample can be set while verifying proper
placement for use of the maximum surface area possible. The sample holder for reflection
measurements is removed from the front of the sample chamber such that the integrating
sphere does not have to be removed. For reflection measurements of powders, the powder
holder is supplied as standard.
IJV-726/IJN-727 Dedicated Gemstone Integrating
Sphere for V-650, V-660 and V-670
The Model IJV-726/ IJN-727 are dedicated gemstone
integrating sphere units designed to measure the transmittance
(diffuse transmittance) and reflectance (diffuse reflectance)
of a sample that diffuses light. In addition, with the various
attachments provided as standard, measurement of precious
stones mounted on rings and necklaces is possible.
Use with the GHP-506 polarizer (option) is recommended.
Model name
IJV-726
IJN-727
Inside diameter of
integrating sphere
60 mm
60 mm
Minimum sample size
2 mm diameter (transmittance
and reflectance measurements)

Peak as a result of
radiation processing
appears at 740 nm.
Maximum sample size 10 mm dia. (Transmittance),
30 mm dia. (Reflectance)
Wavelength range
200 - 870 nm
200 - 2500 nm
Optional Accessories for Solid Samples continued
37
V-600 Series Optional Accessories
for Solid Samples
Integrating Spheres
HISV-728/HISN-729 Portable Integrating Sphere for V-650, V-660 and V-670
An integrating sphere unit is used for measuring turbid liquid samples and opaque
solid samples with a UV-Vis/NIR
spectrophotometer. However it is difficult to
measure such samples at a location remote
from the instrument. With the portable
integrating sphere, monochromatic light is
conveyed from the sample compartment of
the spectrophotometer by an optical fiber
and is irradiated onto a solid or powder
sample. The diffuse reflected light from
the sample is collected efficiently by the
integrating sphere and is introduced into
the detector. This design, which employs an
optical fiber, enables spectra to be acquired
from remote samples. The portable integrating sphere is easy to use because it is compact
and lightweight.
Optical fibers are optional.
Optional OFV-624/625 Optical fiber for HISV-728 (UV-Vis)
Optional OFN-626/627 Optical fiber for HISN-729 (UV-Vis/NIR)
These optical fibers are optional for the remote integrating sphere. You can choose the
wavelength range used and the length of optical fiber.
Please inquire about lengths other than those shown below.
38
Model name
Applicable model
Wavelength range
Length of fiber
OFV-624
HISV-728
250 - 800 nm
1m
OFV-625
HISV-728
250 - 800 nm
2m
OFN-626
HISN-729
250 - 2000 nm
1m
OFN-627
HISN-729
250 - 2000 nm
2m
Model Name
HISV-728 P/N: 6916-J028A
HISN-729 P/N: 6916-J029A
Inside diameter of integrating
sphere
60 mm
60 mm
Window Size
25 mm diameter
25 mm diameter
Wavelength Range
250 - 870 nm
250 - 2000 nm
Optical Fiber Probe Units
FAV-750/FAN-751 Optical Fiber Unit for V-650, V-660 and V-670
The FAV-750/FAN-751 enable the
measurement of bulky samples that cannot
be set in the sample compartment and/or
samples that are in special environments.
The light from the main instrument is
introduced to the optical fiber. The light
from a sample is introduced to an external
detector via the optical fiber.
Optical fiber, fiber connection parts, and
external sample compartment are required
options. The external detector is included as
standard.
Model name
FAV-750 P/N: 6916-J050A
FAN-751 P/N: 6916-J051A
Instrument
V-650/660
V-670
External detector
Photomultiplier tube
Photomultiplier tube and PbS
Wavelength range
250 - 800 nm
250 - 2000 nm
Optional Fiber Connection Port
Select a connection port suitable for the fiber type.
• Bundle type
• FC connector type
• SMA connector type
Fap-754 Optical Fiber Unit for V-650,
V-660 and V-670
This interface accessory is for measurement
using the internal detector of the
spectrophotometer. The light from the main
instrument is introduced to the optical fiber.
The light from a sample is introduced to
the detector of the main instrument via the
optical fiber. Optical fiber, fiber connection
parts, and external sample compartment are
required options.
Elm-742 External Light Source
Interface for V-650, V-660 and V-670
This device is for introducing light from
the sun or other light source into the
spectrophotometer, and measuring the
spectrum of the external source. This
accessory can be used for the evaluation of
light sources.
• For correction of the measured spectrum,
a secondary standard source is also
required.
• The optical fiber is sold separately.
Optional Accessories for Solid Samples continued
39
V-600 Series Optional Accessories
for Solid Samples
Absolute Reflectance Measurement Accessory
This is an accessory for measuring the absolute reflectance of samples that exhibit specular
reflection such as coated metals or glasses, and for measuring the relative reflectance of
samples that exhibit diffuse reflectance. By moving the integrating sphere with the detector,
the reflectance of the sample can be measured while varying the angle of incidence.
The absolute reflectance measurement accessories cannot be used with the V-630. They can
only be used with the V-650/660/670.
ARMV-734/ARMN-735 Automated Absolute Reflectance Measurement
Accessory for V-650, V-660, and V-670
The angle of incidence for the sample, detector position and
polarizer are controlled with the instrument software. Sample
measurement varying the angle of incidence and angle
of acceptance and switching of S and P polarized light is
automated. Since the angle of the sample stage and position
of the detector can be changed independently, measurement
of the absolute reflectance and transmittance while varying
the angle of incidence is possible. A polarizer with PC
automated switching is provided as standard, and in addition
to S polarized light and P polarized light, there is a setting
for N polarized light that obtains the same measurement
results as non-polarized light, enabling the examination of the
polarization properties of samples.
Model Name
ARMV-734
ARMN-735
Instrument
V-650/660
V-670
Wavelength range
250 - 850 nm
250 - 2000 nm
Inner diameter of integrating
sphere
60 mm dia.
Incidence Angle
Absolute reflectance measurement mode: 5˚ to 60˚
Transmittance measurement mode: 0˚ to 60˚
Relative reflectance measurement mode: Vertical incidence
Angle setting
0.1˚ step automatic
Sample dimensions
Absolute reflectance measurement mode:
Minimum 20(H) x 20(W) x 1(T) mm
Maximum 70(H) x 70(W) x 10(T) mm
Relative reflectance measurement mode:
Minimum 20(H) x 20(W) x 0.5(T) mm
Maximum 70(H) x 70(W) x 10(T) mm
40
Accuracy
±1.5% (Absolute reflectance measurement mode, incidence
angle of 6˚)
100% line flatness
Within ±1%
Polarizer
Standard
Standard software
Absolute reflectance spectral measurement, interval
analysis
Optional SSH-508 Solid Sample Holder for ARV-730/ARN-731/ARSV-732/
ARSN-733/ARMV-734/ARMN-735
By installing this on the entrance to the detector of the absolute reflectance measurement
device, the diffuse transmittance of scattering samples can be measured with a vertical (90°)
incidence.
Minimum sample size
30(H) x 30(W) x 0.5(T) mm
Maximum sample size
70(H) x 80(W) x 10(T) mm
Optional Wide Incident Angle Sample Holder for ARV-730/ARN-731/ARSV732/ARSN-733/ARMV-734/ARMN-735
This sample holder is attached to the sample stage to support an angle of incidence up to a
maximum of 85º.
Minimum sample size
30(H) x 60(W) x 1(T) mm
(ARV/ARN)
30(H) x 30(W) x 1(T) mm
(ARSV/ARSN/ARMV/ARMN)
Maximum sample size
70(H) x 100(W) x 10(T) mm
Incident angle
0 - 85º
Phase Difference Measurement Unit for ARV-730/ARN-731/ARSV-732/ARSN733/ARMV-734/ARMN-735
By using an optional angle selective analyzer, measurements of the phase difference of
metal films can be provided. The system is more compact and simpler than a spectroscopic
ellipsometer, while maintaining high precision. It is useful for routine measurement of
semiconductors, optical devices and other materials. Quarter wave plates for evaluation
of optical disks can also be used. The optional accessory consists of an angle selective
analyzer (Birefringent Polarizing Prism) and the phase difference measurement program.
Wavelength range
250 - 850 nm (ARV/ARSV/ARMV)
250 - 2000 nm (ARN/ARSN/ARMN)
Maximum sample size
70(H) x 100(W) x 10(T) mm
Polarization rotation angle
0 - 90º
Analysis of a Notch Filter
Using the interval data analysis program which is the standard software for the automatic
absolute reflectance measurement unit, the wavelength, photometric value and angle of
incidence can be displayed in three dimensional plots.
The figure below outlines the spectral measurement data of a notch filter displayed in three
dimensions. The shift in peak wavelength and changes in peak strength can be rapidly
observed with respect to angle of incidence.
Optional Accessories for
Solid Samples continued
Measurement window
3D display using the interval data analysis program
41
V-600 Series Optional Accessories
for Solid Samples
Absolute Reflectance Measurement Accessory
Variable Incident Angle Measurement of Diffuser Plate
The optional Absolute Reflectance with Variable Incident Angle Measurement Program for
the automatic absolute reflectance measurement unit is a program for performing variable
incident angle measurement at a fixed wavelength. This program supports measurement at
many wavelengths.
17
15
10
600nm
550nm
500nm
450nm
400nm
%T
5
0
-10
42
-8
-6
-4
Angle [deg]
-2
0
The figure at left displays the angle variation
data from angle scanning measurement
of transmitted light from a diffuser when
the detector is moved at 0.1º intervals
from 0º to -10º (10º clockwise) with normal
incidence. The wavelength range measured
was from 400 nm to 600 nm in steps of
50 nm. A tendency for light scattering to
weaken as the angle moves away from
0º can be observed. In addition, although
wavelength dependence of transmittance
is seen at 0º, it is clear that it largely
disappears at around -4º.
ARV-730/ARN-731 Absolute Reflectance Measurement Accessory for V-650,
V-660, and V-670
ARSV-732/ARSN-733 Absolute Reflectance Measurement Accessory for
V-650, V-660, and V-670
The ARV-730/ARN-731 is an accessory type where the movement of the sample stage and
detector is synchronized (Synchronous type). The angle of incidence of the sample can be set
by moving the detector manually, and measurement of the absolute reflectance with varied
angles of incidence.
The ARSV-732/ARSN-733 is an accessory
type where the angle of the sample stage
and position of the detector can be changed
independently (Asynchronous type). The
rotation of the detector and the stage are each
set manually. The absolute reflectance and
transmittance of a sample can be measured
with varied angles of incidence.
With all models, the relative reflectance is
measured with light normally incident on the
sample, which is set behind the integrating
sphere.
Used in conjunction with the ARG-476 polarizer
for the absolute reflectance measurement
device, or the polarizer for the GHP-506 UV/Vis/
NIR, the polarization properties of the sample
can be examined.
Model name
ARV-730
ARN-731
ARSV-732
ARSN-733
Instrument
V-650/660
V-670
V-650/660
V-670
Wavelength range
250 - 850 nm
250 - 2000 nm
250 - 850 nm
250 - 2000 nm
Inner diameter of integrating sphere
60 mm dia.
Incidence angle
Absolute reflectance measurement
mode: 5˚ to 60˚
Transmittance mode is not available.
Transmittance measurement mode:
0˚ to 60˚
Relative reflectance measurement
mode: Vertical incidence
Angle setting
2.5˚
Sample stage: 0.1º
Detector stage: 1º
Sample dimensions
Absolute reflectance measurement mode:
Minimum 20(H) x 20(W) x 1(T) mm
Maximum 70(H) x 100(W) x 10(T) mm
Relative reflectance measurement mode:
Minimum 20(H) x 20(W) x 0.5(T) mm
Maximum 70(H) x 100(W) x 10(T) mm
Accuracy
±1.5% (Absolute reflectance measurement mode, incidence angle of 6˚)
100% line flatness
Within ±1%
Polarizer
Optional
Optional ARG-476 Polarizer for ARV-730/ARN-731/ARSV-732/ARSN-733
The ARG-476 is a Glan-Taylor polarizer for the absolute reflectance measurement unit. Using
a polarizer is recommended in order to measure absolute reflectance accurately at an angle
of incidence of 30º or more. By setting the polarizer at 45º, the same result can be obtained
as for measurement with non-polarized light.
Wavelength range:
250 - 850 nm (ARV-730/ARSV-732)
250 - 2000 nm (ARN-731/ARSN-733)
Polarization rotation angle:
0 - 90º
43
Type 02
Type 03
JASCO INTERNATIONAL CO., LTD.
4-21, Sennin-cho 2-chome, Hachioji, Tokyo 193-0835, Japan
Tel: +81-42-666-1322 Fax: +81-42-665-6512
www.jascoint.co.jp
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