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Catalogue 2014
EPR/ESR
Spectrometer
Catalogue for EPR-Spectrometer,
Accessories & Glassware
Content
Content
EPR / ESR Spectrometer
S. 3
I. What is new? ººBench Top EPR / ESR Spectrometer (Model# MS 5000)
ººTechnical data ººWide field of applications
ººKey features
ººOptional functions (for MS 5000)
3
4
4
5
5
II. Temperature controllers ºº
for MS 5000
ºº
Autosampler add on (MS 5000)
5
5
III. Autosampler for MS 5000
6
6
IV. Goniometer (for MS 5000)
7
V. X-ray Unit
7
VI. Optical Stimulation Unit
7
VII. Optional packages for Special Applications using the MiniScope MS 5000 and MS 400
8
VIII.Standards
8
IX. Optional Software for Spectrometer of the Line MiniScope (MS 5000)
9
X. PC with monitor (Dell)9
Glassware
S. 10
How to choose the appropriate Sample Vessel for your Sample 10
I. Standard Sample Holder 11
ºº
Resonator Protection Tubes
II. Sample Tubes and Closing Caps
11
III. Capillary measurements for liquid samples
12
ººGuidance Tubes
ººCapillary and Sealing Kit
IV. Accessories for temperature control and TC H03 11
ºº
12
12
12
Dewars13
V. Holder and accessories for alanine dosimetry
Optional accessories: Sample Tubes for Dosimetry Tablets
ºº
ººOptional accessories: Blister Holders
2
11
13
13
13
VI. Sample Holders, Flat Cell Cuvette and Tissue Cells 14
VII. Flat Cell Cuvette
14
VIII.Sample storage
15
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EPR/ESR Spectrometer
What is new?
Bench top EPR/ESR Spectrometer
(Model# MS 5000)
Module # 1000
compact size
the ideal ‘everywhere’ spectrometer, can be handled
by one person, low electricity power consumption
45 kg, 397 x 262 x 192 mm
sensitivity & stability
outstanding sensitivity and magnetic field
stability, extended data detection schemes
versatile options
detection limit of 10 nM in PBS
automated goniometer for measurement of angular dependencies, low temperature measurements, big collection of specialized sample holders and
glassware, comfortable software for data handling and evaluation
autosampling for liquids, powder and solid samples
Facts
Magnet system
ººcompact, bench top spectrometer at highest sensitivity
ººg-Factor representation of EPR spectra
ººkinetic measurements, time resolution down to 2 µs
ººexcellent field stability
ººextremely stabel AFC (automatic frequency control),
ººautomatic field readjustment according to microwave frequency
ººextreme stability of all measurement parameters allowing for
ns response time
long range accumulation up to weeks
RF system and signal channel
measurement frequency stability better +/- 3 Hz @ 10 GHz
3
EPR/ESR Spectrometer
Technical data
Magnetic field
Microwave
field modulation
1 μT – 1 mT
frequency
9.2 – 9.6 GHz
modulation frequency
100 kHz
power
1 μW – 100 mW
magnetic field range
5 - 650 mT
resonator
rectangular TE 102.
Automatic detection of Q factor.
field resolution 0.03 µT
sensitivity
8 x 109 spins / 0.1 mT
scan range
0 – 645 mT
case
size 397 x 262 x 192 mm
scan resolution 120,000 steps
weight
~ 45 kg
stability
1.5 µT / min, 10 µT / h
power consumption
~ 300 VA
homogeneity
+ 5 μT within sample region
sweep time
1 s – 2 h
cooling
air if necessary (low sound)
Wide field of applications
Life sciences
Food Chemistry and Pharmacy
Petrochemistry
Nitric oxide measurement, reactive oxygen
Antioxidative features of foodstuff,
Living polymers, nitroxide quantification,
species, oxidative stress, radical generating
radicals in foodstuff, radiation-induced
radicals in varnish, UV stability of scratch
systems, photo dynamic therapy
radicals
resistant varnish
Environmental Toxicology
Alanine Dosimetry
Separation of Radicals
Generation of radicals by particles
Alanine dosimetry (tablets, thin films)
EPR/HPLC coupling
Biophysical Features
Bioinorganic Chemistry
Cosmetic
Oxymetry, membrane fluidity, pH in
Bioinorganic transition metal com-
Radical protection factor, protection
microenvironment, viscosity, phase
pounds, fenton chemistry, effect of
features of UV-filters in creme, sham-
partition
heavy metal ions on living tissue
poo, etc.
Kinetic of ROS generation by
xanthine / xanthine oxidase
4
Basal (black) and stimulated
(blue) NO generation by rat aorta
TEMPOL in a two phase system
oil/water
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Spectrum of an Alanine tablet
irradated with 5 Gy
Key Features
MiniScope MS5000
Large Spectrometer
3 3 4 .8
3 3 5 .8
3 3 6 .8
3 3 7 .8
3 3 8 .8
M ag n etic F ield S tren g th [m T ]
100 µM TEMPOL
SOD mimetic Cu (II) complex
NO-Hemoglobin
Weak pitch: P = 12 mW,
modulation = 0.2 mT
Detection limit
available Standards
Ultra fast and stable
Sensitivity of MS 5000
10 µM in PBS
Chromium and Manganese
auto frequency control
bench top spectrometer
Wide magnetic field range
No spikes due to liquid
(650 mT)
nitrogen bubbles
Optional functions (for MS 5000)
Extended RF Power range
Module # 1011
10 µW – 100 mW
Recommended for samples saturating at microwave power
higher than 50 mW and for measurements with microwave
power less than 100 µW.
Extended Magnetic Field range
compared to large stationary spectrometer with
universal TE 102 cavity
II. Temperature controllers
for MS 5000
Bio Temperature Controller
Module # 1102
For temperature stabilization of biological
systems like tissue samples.
Temperature range: 290 K – 323 K
Module # 1012
50 – 600 mT air cooled
The increase of magnetic field range is recommended to measure transition metal complexes of Fe(III) low spin. The extension
includes a compact refrigerated circulator with 240 W cooling
Vessel for flushing resonator
Module # 1103
Liquid nitrogen evaporates, flushing resonator and preventing the condensation of humidity, optional
accessory in connection with fixed temperature dewar.
capacity.
g factor Evaluation,
Module # 1013
including Frequency Counter
ººFrequency range: 0.5 to 10.0 GHz
ººInput level: -5 to +10 dBm, 50 Ohms
ººDisplay: 7 digits, 1 kHz resolution
ººConnector: SMA female, back panel
5
EPR/ESR Spectrometer
Temperature Controller TC H03
Module # 1104
Autosampler for Liquids down to 1 ml
incl. Table
Suitable for 23 liquid samples and
Temperature range: 103 K – 473 K
cleaning liquid.
PC controlled (control / registration software included)
Automatic liquid sample transfer for
Optional: Software for automatic data acquisition for
spectrometers of the MiniScope series,
temperature measurements.
Autosampler and integrated peristaltic
Module # 1203
pump, software controlled. Software
for kinetic measurements, including
automatic data acquisition and data
evaluation. Single or multiple chemi
cals, optional: automa
ted component mixing and temperature
control.
III. Autosampler for MS 5000 (select one)
Autosampler for powder and
Autosampler add on (MS 5000)
Module # 1201
solid samples
Two pump system,
automated handling of up to
Reaction Chamber
Module # 1204
23 samples in quarz tubes
3 – 6 mm diameter, precise
Autosampler Rack
height positioning within
for continuous flow systems
Module # 1205
resonator for highest
Peristaltic Pump
reproducibility.
Module # 1206
Very uniform pump behaviour,
suitable for kinetics measurements.
Autosampler for Liquids up to 43 ml
Module # 1202
Thermostat Block
Module # 1207
Suitable for 14 liquid samples and
Max. temperature 130° C
cleaning liquid.
ººAdapted sample tubes for optimum heat transition
ººInterchangeable metal block for different sample vessels
Automatic liquid sample transfer for
spectrometers of the MiniScope series,
Autosampler and integrated peristaltic
Autosampler for special
pump, software con
trolled. Software
applications
for kinetic measurements,
Suitable for beer quality and
including automatic data
shelf time analysis
acquisition and data evaluation. Single or multiple
chemicals, optional: automated component mixing
and temperature control.
6
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Module # 1208
IV. Goniometer (for MS 5000)
Automated loading of
Automated Goniometer
ºº
Module # 1300
Module # 1401
X-ray Source
Fully automated angular rotation
of sample
ººStep size 0,1 to 180°
ººEach measurement of a new spectrum starts with an auto-
mated readjustment of the spectrometer for best measurement performance
Ionization Chamber for
Module # 1402
X-ray dose measurements
fully integrated dose measurement for precise monitoring
of X-ray irradiation intensity
Automated Goniometer
Module # 1301
for Autosampler
ººFully automated angular rotation of sample with special
adaptations for Autosampler
ººStep size 0,1 to 180°
ººEach measurement of a new spectrum starts with an auto-
mated readjustment of the spectrometer for best measurement performance
VI. Optical Stimulation Unit
Integrated Light Source
Module # 1501
For sample treatment or for triggering
kinetic experiments.
V. X-ray Unit
X-ray Source
ºº
Module # 1400
fully automated 50 kV
External Irradiation Unit:
X-ray tube at a focal spot
Halogen Lamp (Hamamatsu)
size of 1 mm
Wavelength range: 240 – 700 nm, depend of the lamp inclusive
ººHigh voltage generator
ººShielding integrated
Module # 1502
light guide adapter at the MiniScope (additional wavelengths
available on request)
7
EPR/ESR Spectrometer
VII. Optional packages for Special
Applications using the MiniScope
Reactive Oxygen Species (ROS)
MS 5000 and MS 400
Consisting of:
Alanine Dosimetry
ººFlat cell cuvette FZK 160-7x0.3
ººSpecial holder SH-P for flat cell cuvettes, guidance tubes,
Module # 1601
Consisting of:
ººSpecial holder SH-P
ººSpecial cuvette FFZK 130-4,5x0.8 for dosimetry thin
film test strips
Module # 1603
tissue cells and special cuvettes
ººFixed holder with fitting for flat cell cuvettes FZK 160-7x0.3
ººFixed holder with fitting for guidance tube F 120 / 1.5
ººOptional: Software for automatic data acquisition and
evaluation of kinetic measurements using disposable glass
capillaries.
ººHolder for precise sample positioning with
Includes:
adjustable altitude
ºº2 Tubes for dosimetry tablets with inner diameter
4701, 4601, 4602
4,0 mm or 4,8 mm
ººOptional: Software for dose calculation against a reference
(Aerial Company). Price upon request.
Includes:
4601, 4704, 4101, 4701, 4606
VIII. Standards
Holder and positioning system
Module # 1701
for Standard Samples (MS 5000)
NO-Fe(II)DETC (Fixed temperature
Module # 1602
dewar for N2 liquid)
MT Weak pitch Cr in MgO
Consisting of:
ººFixed temperature dewar for spectrometer of the line
MiniScope
ººHolder fit to the device with adjustable altitude for fixed
temperature
ººdewar suitable for MiniScopes
Includes:
4606
8
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Module # 1702
# ????
Secondary standard Chromium
Module # 1703
Automated data evaluation,
Module # 1804
Cr3+ in MgO
angular dependent peak evaluation
With protocol on g-factor
An example showing angular dependent measurements with
Goniometer. Evaluation of peak position vs. angle. Different
spot sizes mark different peak intensities on a ln-scale.
Secondary standard Manganese
Module # 1704
Mn2+ in ZnS
Software capability for storage of
With protocol on g-factor and peak distances
Module # 1805
calibration curves for quantitative
measurements and dosimetry
X. PC with monitor (Dell)
PC with monitor (Dell)
Module # 3000
IX. Optional Software for Spectrometer
of the Line MiniScope (MS 5000)
Automatic data acquisition and
Module # 1801
evaluation for kinetic measurements
Sequence editor for free
Module # 1803
programming of automation
functionality
9
Glassware
Glassware
How to choose the appropriate sample vessel for
In the case you use organic solvents with a lower dielectric constant;
your sample?
you can use capillaries with a higher volume or sample tubes.
For measurement of samples in the EPR spectrometer you have
the choice between the following sample vessels:
Sample tubes are used for liquid and solid samples. We offer
ººCapillary
ººSample tube
ººFlat cell
ººTissue cell
ººSpecial cuvette
ººFixed temperature dewar
sample tubes of different diameters and length. The dielectric
Some general remarks
fer. In the case a sample is not tunable you can either choose a
The active volume in the vertical direction of the MiniScope rect-
sample tube with a smaller diameter or you reduce the amount
angular resonator is 23 mm. Over this 23 mm you have a Gauss
of sample inserted into the resonator.
constant of the solvent of your liquid sample lays down the dia
meter of the sample tube. For example radicals in hydrocarbons
can be measures using a 4 mm or 5 mm sample tube. Concer
ning the solid samples the humidity of the sample restricts the
diameter of the sample tube. The length 135 mm is used in gene
ral. The length 240 mm is adapted to the finger dewars, we of-
distribution of the intensity. This means in the centre you measure 100 % intensity. 1.65 mm top and bottom of the centre
Flat cells are used for liquid samples in solvents with a high
the intensity is 95 %. 2.5 mm top and bottom of the centre the
dielectric constant. The ideal sample distribution in the resonator
intensity is 90 %. This means you have two possibilities to get
leads to a 2 – 2.5 fold higher intensity compared to 50 μl capilla
reproducible and comparable measurements:
ries. The crucial point, when using a flat cell is the orientation of
the flat section, the vertical position and the filled volume of the
[1] You fill the sample vessel over the whole active volume of the
resonator.
flat cell. Therefore we recommend using the special holder SH-P
as the system to achieve always the same sample positioning.
[2] You fill 5 mm of your sample vessel. In this case you have
to make sure that the sample is positioned absolutely in the
Tissue cells can be used to measure skin sections, paste and
centre of the resonator. A further requirement is that in the
emulsions. The same requirements have to be fulfilled that are
case you are using different sample vessels of the same kind,
valid for flat cells.
that they are selected for the same intensity or that you have
compared them using a standard solution introducing correc-
A special cuvette we offer for alanine dosimetry of thin film
tion factors.
test strips. This cuvette has a flat section too. Furthermore we
offer special tubes for alanine tablet dosimeters.
In the case you are using sample vessels with a flat section you
can orientate the flat section parallel or perpendicular to the
Fixed temperature dewars are used to measure samples
front panel of your spectrometer. Please try, which orientation
at liquid nitrogen temperature of 77 K. You can either drop the
gives the higher intensity.
sample into the liquid nitrogen and the frozen drops are collected in the finger for measurement.
Capillaries are standardized calibrated sample vessels. They are
A further possibility is to freeze a cylinder of 4 mm diameter
cheap one way items and allow an easy sample exchange. Usually
matching to the size of the inner finger of the dewar. In the case
capillaries are used together with a matching guidance tube. For
you like to measure solid samples you can use sample tubes of 3
aqueous solutions the maximum volume of capillaries is 50 μl.
or 4 mm outer diameter and 240 mm length.
10
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I. Standard Sample Holder
II. Sample Tubes and Closing Caps
For the alignment of sample tube within the standard sample
holder for ESR measurements.
Sample tube,
Module # 4300
synthetic quartz P 165 / 3
Length: 165 mm, Outer diameter: 3 mm, Wall thickness: 0.7 mm
(Requires Module # 4200)
PH-3 for sample tubes 3 mm
Module # 4200
PH-3.6 for guidance tubes 3.6 mm
Module # 4201
PH-4 for sample tubes 4 mm
Module # 4202
(Requires Module # 4202)
PH-5 for sample tubes 5 mm
Module # 4203
Sample tube,
Sample tube,
Module # 4301
synthetic quartz P 165 / 4
Length: 165 mm, Outer diameter: 4 mm, Wall thickness: 0.7 mm
Module # 4302
synthetic quartz P 165 / 5
PH-6 for sample tubes 6 mm
Module # 4204
autosampler tube grip
Module # 4205
Length: 165 mm, Outer diameter: 5 mm, Wall thickness: 0.7 mm
(Requires Module # 4203)
for sample tubes 3 mm
Sample tube,
Other sizes are available on request
synthetic quartz P 165 / 6
Module # 4303
Length: 165 mm, Outer diameter: 6 mm, Wall thickness: 0.7 mm
(Requires Module # 4204)
1 Set closing caps 4 mm
Module # 4310
(50 pieces)
Resonator protection tubes
Resonator protection tube
Module # 4003
1 Set closing caps 5 mm
Module # 4311
(50 pieces)
11
Glassware
III. Capillary measurements
IV. Accessories for temperature
for liquid samples
control and TC H03
Guidance tubes
Holder fit to the device with
Module # 4100
adjustable altitude for fixed
Guidance Tube F-120 / 1.5
Module # 4005
# 4002
temperature dewar suitable for MiniScopes.
To geometrically align the capillary in the
center of the resonator. See # 4009, requires # 4200
Capillary and Sealing Kit
Disposable glass capillaries 50 µl,
Guidance Tube F-120 / 1.5T
Module # 4006
Sample Holder for TC H02
Module # 4102
Module # 4009
250 pieces
Volume 50 μl with ring,
accuracy < +/- 0.25 %,
precision < 0.5 %
and TC H03 (Top)
(capillaries with smaller volumes are
available upon request)
Sealing kit for disposable glass
Module # 4010
capillaries
None drying out vinyl kit on one plastic plate for secure sealing
of more than 75 capillaries
12
Sample Holder for TC H02
and TC H03 (Bottom)
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Module # 4103
V. Holder and accessories
Resonator Dewar for Temperature
Module # 4104
Controller TC H02 and TC H03
for alanine dosimetry
Holder for precise sample
Module # 4000
positioning with adjustable altitude
(e.g. alanine tablets)
Also see Special Holder: Module # 4500
Resonator Dewar for Bio
Module # 4105
Temperature Controller BTC 01
and BGC 01
Optional accessories
Sample Tubes for Dosimetry Tablets
For alignment of dosimetry tablets
Type 1: Inner diameter 4,0 mm
Module # 4001
Type 2: Inner diameter 4,8 mm
Module # 4002
Dewars
To manually cool the samples
Dewars for spectrometer
Module # 4130
of the line MiniScope
Volume 50 ml
ºº
ººOuter finger: outer diameter 10.5 mm, length 178 mm
ººInner finger: inner diameter 5.4 mm, length 105 mm
ººStorage time 2 h
Blister Holders
Models with larger volume and different sizes of the finger
upon request.
Blister Holder, Type S
Module # 4601
13
Glassware
VI. Sample Holders, Flat Cell Cuvette
and Tissue Cells
VII. Flat Cell Cuvette
For Aqueous solutions
Special holder SH-P for flat
Module # 4500
Flat cell cuvette FZK 160-7x0.3
cell cuvettes, guidance tubes,
Width of flat section 7.0 mm,
tissue cells and special cuvettes
width of gap 0.3 mm
Module # 4502
One fixed holder with fitting (separately available too, see below), especially adapted as one chooses one of the following
sample vessels:
ººFlat cell cuvette FZK 160
ººFlat cell cuvette FZK 200
ººGuidance tube F-120 / 1,5
ººTissue cell GZ 170
ººSpecial cuvette FFZK 130-4,5x0,8
ººHolder mounted at the device with fixation and protection
Flat cell cuvette FZK 200-5x0.3
Module # 4503
Width of flat section 5.0 mm,
width of gap 0.3 mm, including 5 closing caps 5 mm
tube for spectrometer of the line MiniScope allows a
definite sample exchange
Flat cell cuvette FZKV 200-7x0.3
Module # 4504
Width of flat section 7.0 mm,
width of gap 0.3 mm
Fixed holder with fitting for flat
Module # 4501
cell cuvettes, guidance tubes,
tissue cells and special cuvettes
Especially adapted to the following sample vessels:
ººFlat cell cuvette FZK 160
ººFlat cell cuvette FZK 200
ººGuidance tube F-120 / 1,5
ººTissue cell GZ 170
ººSpecial cuvette FFZK 130-4,5x0,8
Adapted to special holder SH-P
Special cuvette FFZK 130-4,5x0.8
Module # 4505
for dosimetry test strips
The special cuvette consists
of a tube of 95 mm length,
whose bottom end has a flat
part of a length of 35 mm with
one open side and a gap of 0.8
mm. The width of the flat section and the inner diameter of
the tube can be chosen in dependency on the width of the test
strip. For exact positioning of the cuvette we recommend our
special holder SH-P.
14
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VIII. Sample storage
Tissue Cells
Sample storage
For tissue samples
Module # 4700
Recommended for safe and organized
storage of sample tubes
The tissue cell consists of a flat part (sample holder) with the
dimensions 65 x 7 x 2 mm having two holders in axial direction
(bottom length of holder 20 mm, upper length 85 mm). The
flat part has a milled deepening (length 30 mm, width 5 mm,
depth in general 0.3 mm) which serves to hold the sample. The
delivery includes one cover slide and two clamps for fixing the
cover slide. Additional cover slides and clamps can be ordered
separately.
Tissue cell GZ 170-5.0x0.3
Module # 4506
Width of deepening 5.0 mm, depth 0.3 mm
Tissue cell GZ 170-5.0x0.5
Module # 4507
Width of deepening 5.0 mm, depth 0.5 mm
1 Cover slide for tissue cell GZ 170
Module # 4508
1 set (2 pcs.) clamps
Module # 4509
for cover slide of tissue cell
Tissue Cell GZ 170P
Module # 4510
(Plastic)
Material: plastic POM / PTFE. For fast and simple sample exchange for series measurements. The tissue cell consists of a
flat part (sample holder) with the dimensions 50 x 5 x 2 mm
having two holders in axial direction. The flat part has a milled
deepening with dimensions 13 x 5,8 mm, depth 0,2 mm and
a additional deepening with diameter 5,8 mm, depth 1 mm.
15
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Competence in metrology
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Inline Metrology (PV/microelectronics)
OSL/TL reader “lexsyg”
In- and offline tools for semiconductor defect recognition
and steady state or µPCD carrier lifetime measurements
with fast decays down to below 100 ns
Versatile research equipment for optically and thermally
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measurements, spectroscopic and camera (EMCCD) options. For dating applications, dosimetry and beyond
MDPinline ingot
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Contact
Sales office:
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Freiberg Instruments
Production and R&D:
magnettech GmbH
Freiberg Instruments GmbH
Louis-Blériot-Str. 5
Delfter Str. 6
D-12487 Berlin, Germany
D-09599 Freiberg, Germany
Phone: +49 30 6780 2526
Phone: +49 3731 419540
Fax:
Fax:
+49 30 6322 4101
+49 3731 4195414
E-Mail:[email protected]
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