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“Basic Cell Sorter Training using BD FACSAriaTM III”
Workshop Agenda
MultiPark, BMC B12, Lund University
September 21 - 24, 2015
Day 1 - Introduction to flow cytometry & the cell sorter
9.00 -10.30
10.30 -10.45
This is the FACSAria cell sorter – Presentation & startup of the instrument
10.45 -12.00
Break
Lecture: ”Flow cytometry – Basic concepts & technical solutions ” (Seminar room B1244b)
12.00 -12.45
Lunch
12.45 -14.15
Basic experiment design & data collection – Demo + hands-on (Group 1)
14.15 -14.30
Break
14.30 -16.00
Basic experiment design & data collection - Demo + hands-on (Group 2)
16.00 -16.15
Instrument shutdown procedure - Hands-on (Group 2)
Day 2 - Multicolor flow cytometry
9.00 - 10.30
10.30 - 10.45
Instrument startup & sample preparation (Group 2)
10.45 -12.00
Break
Lecture: ”Multicolor flow cytometry” (Seminar room B1244b)
12.00 -12.45
Lunch
12.45 -14.15
Seven color experiment set-up & optical spillover comp. - Demo + hands-on (Group 2)
14.15 -14.30
Break
14.30 -16.00
Seven color experiment set-up & optical spillover comp. - Demo + hands-on (Group 1)
16.00 -16.15
Instrument shutdown procedure - Hands-on (Group 1)
Day 3 - Cell sorting
9.00 - 10.30
10.30 - 10.45
Instrument startup & sample preparation (Group 1)
10.45 -12.00
Break
Lecture: ”Charged droplet cell sorting - theory & practice” (Seminar room B1244b)
12.00 -12.45
Lunch
12.45 -14.15
Sort setup & sorting - Demo + hands-on (Group 1)
14.15 -14.30
Break
14.30 -16.00
Sort setup & sorting - Demo + hands-on (Group 2)
16.00 -16.15
Instrument shutdown - Hands-on (Group 2)
Day 4 - Flow cytometry data analysis
9.00 - 10.30
10.30 - 10.45
Instrument startup & sample preparation (Group 2)
10.45 -12.00
Break
Lecture: ”Flow data - analysis, integrity & publication ” (Seminar room B1244b)
12.00 -12.45
Lunch
12.45 -14.15
Sorting into cell culture plates + Build your own sort project - Demo + hands-on (Group 2)
14.15 -14.30
Break
14.30 -16.00
Sorting into cell culture plates + Build your own sort project - Demo + hands-on (Group 1)
16.00 -16.15
Instrument shutdown - Hands-on (Group 1)
Sverker Segrén – Segrén Flow Cytometry Support AB
Org. Nr. 556851-9747