PHYTOPATHOLOGY HOME PAGE Phytopathology Author’s Guide for Manuscript Preparation 2015 Appropriateness for Phytopathology Phytopathology publishes original research that significantly advances our knowledge of plant diseases, the agents that cause diseases, the factors that influence diseases, and the measures that can be used to control them. Subject matter is not a criterion for judging the merit of an article for publication in Phytopathology. However, it is the author’s duty to establish relevance to the science of plant pathology. Distinctions will be made between what is routine and what is significant. The author should clearly present the significance of the work in the article. The American Phytopathological Society (APS) publishes three journals, each with a prescribed scope that should be considered in determining the most appropriate journal for a manuscript. Please rely on recently published issues for guidance on suitability for Phytopathology. Manuscripts should fit into one of these sections: Analytical and Theoretical Plant Pathology, Bacteriology, Biochemistry and Cell Biology, Biological Control, Disease Control and Pest Management, Ecology and Epidemiology, Etiology, Genetics and Resistance, Human Pathogens on Plants, Mycology, Nematology, Plant Stress and Abiotic Disorders, Population Biology, Postharvest Pathology and Mycotoxins, Techniques, and Virology. Genomics and functional genomics-related papers are encouraged; however, authors must demonstrate how the research addresses the central emphases of Phytopathology described above. Papers that solely report complete or partial nucleotide sequences including draft genome sequences of organisms are not acceptable for publication unless they relate structure to function or demonstrate how the sequence information may advance our understanding of the biology of the pathogen. Methods-type marker papers should meet one of the following criteria: (i) a report of new techniques or modifications that significantly enhances current techniques or the application of these methods, or (ii) the use of techniques to investigate interesting biological questions that contribute to the basic concepts and understanding of plant pathology. Studies of pathogen population biology should address an original question or hypothesis. In general, studies that describe pathogen diversity or population structure are not acceptable unless they also address a biological question that advances our conceptual knowledge of pathogen population biology. In addition, as much care should be given to the development and description of an appropriate pathogen sampling scheme as is given to the subsequent procurement and analysis of marker data. However, sampling deficiencies beyond the control of the author will be acceptable in some cases, e.g., when conducting analyses of historical isolate collections. Papers dealing mainly with taxonomy, such as descriptions of new taxa, should generally be submitted to a taxonomic journal. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may be submitted to Phytopathology. Authors are encouraged to contact the appropriate Senior Editor prior to submission of a manuscript to receive clarification concerning the suitability of their manuscript for publication in Phytopathology. Names and e-mail addresses of the Senior Editors can be found on the first page of each issue. The subject area responsibilities for the Senior Editors are as follows: Gabriele Berg: bacterial ecology in the phyllosphere and rhizosphere, plant–bacteria interactions, microbe–microbe interactions, and biology of human pathogens on plants; Saul Burdman: bacteriology, molecular plant–bacteria interactions, virulence determinants of plant-pathogenic bacteria, and management of bacterial plant diseases; David Cooke: ecology, epidemiology, population biology of fungal and oomycete pathogens; Nicole Donofrio: fungal and oomycete genetics and genomics, molecular fungal and oomycete–plant interactions, and biology of fungal and oomycete pathogens; Karen A. Garrett: disease management, ecology, epidemiology, microbial communities, networks, social-ecological systems, and statistics; Stewart M. Gray: virus–vector interactions, virology, virus diseases of cereal and vegetable crops, virus disease management, virus resistance, and applied virus disease epidemiology; Teresa Hughes: fungi, oomycetes, disease management, population biology, and pathogen–host interactions field crop diseases; Kelly L. Ivors: oomycetes, fungicide resistance, population genetics, vegetable, and ornamental diseases; Steven J. Klosterman: senior editor for reviews (by invitation only); Jeffrey A. Rollins: genetics of host–pathogen interactions, genomics, and fungal biology; Karen-Beth G. Scholthof: virology, viruses of grasses and model systems (especially Brachypodium and Setaria), virus–satellite interactions, host responses to virus diseases, host–virus co-evolution, and molecular virus–plant interactions; Ivan Simko: genetics of host resistance, quantitative disease resistance, linkage and association mapping; Gary E. Vallad: biology and management of vegetable diseases, biology of soilborne plant pathogens, biological control, and disease management; Frank van den Bosch: population dynamics and evolutionary ecology of plants and pathogens, and quantitative modeling; Martin Wubben: nematology, molecular aspects of plant-parasitic nematode biology, nematode–plant interactions, and management of nematode diseases; Chang-Lin Xiao: fungicide resistance, fungal biology, postharvest pathology, disease management, and diseases of fruits and nuts. Authors are strongly encouraged to have at least one colleague review the manuscript before submitting it for publication. Senior Editors may find the content of a submitted paper unsuitable for Phytopathology and return the paper to the author without review. Each manuscript receives two simultaneous reviews. Authors may recommend individuals to review a manuscript, and they also may ask that certain individuals not review a manuscript. Additional experts are consulted as necessary to confirm the scientific merit of any part or all of a manuscript, with due consideration for prepublication confidentiality. Each reviewer makes a specific recommendation to the Senior Editor for the manuscript, based on the following applicable aspects: Importance of the research Originality of the work Analysis of previous literature Appropriateness of the approach and experimental design Adequacy of experimental techniques Soundness of conclusions and interpretations Relevance of discussion Clarity of presentation and organization of the article Demonstration of reproducibility In addition to original research, Phytopathology also publishes the following manuscript formats: Letters to the Editor Letters to the Editor explain, amplify or otherwise comment on research published in the Journal or elsewhere. The Editorial Board reserves the right to reject or publish letters of rebuttal when appropriate. 1 Symposia Symposia from an APS annual meeting may be published in Phytopathology subject to the review process and to the policies, procedures, and page fees applicable to other articles. It is the responsibility of the committee chair sponsoring the symposium to contact the Editor-in-Chief before the symposium is presented. Reviews Reviews provide a survey of a subject relevant to plant pathology with an emphasis on the previous 5 years. Unlike basic research articles for Phytopathology, Reviews should also be easily accessible to nonspecialist scientists and advanced students. Reviews are typically invited by the Senior Editor for Reviews or the Editor-in-Chief. Suggestions for future Reviews are welcome. Page Fees Because of the high cost of publishing, page fees are mandatory and are subject to change without notice. Current fees are $50 per printed page for the first six pages and $80 per printed page for each additional page for members of APS and $130 per printed page for nonmembers. In addition, there is a $20 fee for each black-and-white figure or line drawing. If you would like your images to appear in color in print, the color fees are $500 for the first illustration, $500 for the second illustration, and $250 for the third and each subsequent color illustration in one article. Single Article Purchases PDFs of articles published within the last 12 months may be purchased online. Go to http://apsjournals.apsnet.org/loi/phyto to locate the article and then click the PDF link. APS retains full copyright to any article that is purchased or retrieved with or without payment and all copyright laws apply to any subsequent usage. Open Access All content of Phytopathology is open access without restriction 12 months after publication. Immediate open access can be purchased for any article in Phytopathology. The cost is $1,900 for this option. This fee is in addition to the usual publication fees. Authorship Those who submit papers to Phytopathology should respect the value of the research of their peers by not devaluing authorship. Each author should have made a substantial intellectual contribution to the design, conduct, analysis, and/or interpretation of the study. Each author must approve the final version of the article to be published and be willing to take public responsibility for their contribution to the paper. In addition, the first author and the corresponding author are expected to be able to take public responsibility for the entire paper. Preprints Authors are allowed to present and discuss their findings ahead of publication at scientific conferences, on preprint servers (such as arXiv, bioRxiv, or PeerJ preprints among others), public databases, blogs, and other forms of social media. This prior disclosure does not constitute prior publication. When submitting a manuscript for review, authors need to disclose preprints, databases, proceedings or other preliminary communications or depositories. However, abstracts, presentations or other social media need not be disclosed. Authors may post a link from the preprint archive site to the published abstract posted at the APS journal site once the paper is published. 2 Online Article Enhancements (e-Xtras) e-Xtras are an optional service to enhance the online version of articles or to include supplementary materials. The presence of e-Xtras is noted by a logo and footnote on the first page of the printed article. These e-Xtras may be referenced within the manuscript, but because they are intended to be supplemental in nature and not necessary to the understanding of the printed article, no more than one callout per e-Xtra is allowed. e-Xtra options include: Tables, figures, lists, movies, or other files that provide important additional but supplementary information, which will be linked from the online table of contents, for $20 each. External links from the online table of contents to public databases, such as GenBank or other approved websites. Authors should submit links with the article for review, noting specifically that they wish them to be e-Xtra links. The first five links are added free of charge and additional links are $5 each. PREPARING A MANUSCRIPT Organization of Text Major sections after the introductory statements are Materials and Methods, Results, Discussion, and Literature Cited. General techniques and methods are described in Materials and Methods; brief descriptions of experiments and trials are given in Results. (Subheadings may be used, but avoid excessive fragmentation of the text.) Footnotes to the text are not permitted. Please refer to published articles for standard formatting of heads and subheads. Authors are expected to list all sources of funding for the research project in the acknowledgments section of the manuscript at the time of submission. Scientific Nomenclature and Language The scientific language used in manuscripts should be internally consistent. Please follow The ACS Style Guide (Dodd 1997) or ASA, CSSA, and SSSA Publications Handbook and Style Manual (1998). The Scientific Style and Format (CBE Style Manual Committee 1994) by the Council of Biology Editors provides background on the origins of scientific terms and the governing bodies that rule on current nomenclature in a given field. Apparatus and materials. Names of unusual proprietary materials and special apparatus should be followed by the manufacturer’s name and location in parentheses (e.g., manufacturer, city and state [United States] or city and country). It is only necessary to cite these materials by specific name if the work cannot otherwise be replicated. When necessary, trade names may be used and should be capitalized; trademark symbols should not be used and will be deleted before publication. Authorities for Latin binomials. Citation of authorities for Latin binomial names is optional but appropriate for manuscripts dealing with taxonomy or nomenclature or for organisms with unfamiliar binomials. When used, authorities should be provided at first mention of the organism only. Bacteria. Spellings should be based on Bergey’s Manual of Systemic Bacteriology (Garrity and Boone 2001), the Approved Lists of Bacterial Names (Skerman et al. 1989), or the lists of species published in the International Journal of Systemic Bacteriology (IJSB). Based on Bergey’s Manual, groups below the level of subspecies should be italicized. Where applicable, designate strains. Common names of plant diseases. For common names of plant diseases, the list developed by the APS Committee on Standardization of Common Names for Plant Diseases should be used (http://www.apsnet.org/publications/commonnames/Pages/Approved CommonNames.aspx). Chemicals, chemistry, and biochemistry terms. The ACS Style Guide (Dodd 1997) describes conventions in chemistry and biochemistry. The Merck Index (O’Neil 2006) and Hawley’s Condensed Chemical Dictionary (Lewis 2007) are good sources for spellings of chemical terms. List fungicides by their approved common or generic names. Trade names may be included within parentheses, but do not include manufacturers. See the current issue of Farm Chemicals Handbook (Anonymous) or the most recent edition of Acceptable Common Names and Chemical Names for the Ingredient Statement on Pesticide Labels (Environmental Protection Agency, Pesticide Regulation Division). Cultures. Indicate the source of cultures. Include culture designations obtained from or deposited in recognized collections. Authors are required to deposit voucher cultures and specimens at recognized institutions and to provide accessions numbers in the text. Papers are accepted on the condition that cultures, plasmids and similar materials will be available for distribution to all qualified members of the scientific community, either directly from the investigator or by deposit in national or international collections. Enzymes. Use the enzyme names recommended in the latest issue of Enzyme Nomenclature (International Union of Biochemistry and Molecular Biology 1992). Give the number (classification) of the enzyme at its first use (e.g., EC 1.1.75.6). Genetics. Rieger et al. (1991), Stenesh (1989), and King et al. (2006) are good specialized genetics and molecular biology dictionaries. Fungi. The preferred sources for common and scientific names and authorities of fungi is Fungi on Plants and Plant Products in the United States (Farr et al. 1989) and Ainsworth and Bisby’s Dictionary of the Fungi (Kirk et al. 2008). Insects. Common Names of Insects and Related Organisms (Bosik 1997) can be used to verify insect names. Nucleotide or amino acid sequences. All sequence data must be deposited in GenBank or comparable publically available databases and accession numbers provided. If the sequence is not available for general access from the database when the manuscript is submitted, a Word or PowerPoint file or copy of sequences described in the manuscript should be provided for reviewers and posted as e-Xtra when the manuscript is published. AFLP, SSR or similar types of analysis. Data files used in the analysis of AFLP, SSR, or SNP markers must be provided as supplementary files that will be uploaded on the journal website and made available for readers to download. The format should be the same used for data analysis and run parameters for the analysis provided. Phylogenetic trees. Authors are required to deposit phylogenetic trees and data in TreeBASE (http://treebase.org) and cite accession number(s), or to upload as supporting data. Plants. Farr et al. (1989) is a good source for spellings of common and scientific names. Other good sources include Merriam-Webster’s Collegiate Dictionary, A Checklist of Names for 3,000 Vascular Plants of Economic Importance (Terrell et al. 1986), and The Plant-Book (Mabberley 1997). Use the term “cultivar” for cultivated agronomic and horticultural varieties. Identify the source of the cultivars and include CI and PI numbers when appropriate. The name of a cultivar may be enclosed in single quotation marks. You may also want to refer to http://plants.usda.gov. Software. Software used should be treated as proprietary material or equipment. Give the manufacturer or developer name and location parenthetically within the text. Software should not be listed in Literature Cited. Unpublished software or scripts has to be uploaded as supporting information. Statistics. Papers published in Phytopathology are diverse, and it is not possible to give specific statistical recommendations, guidelines, or rules that can apply to all manuscripts. General guidelines and recommendations are given here that are relevant for a large number of situations. Most importantly, describe statistical methods with enough detail to enable a knowledgeable reader to verify the reported results, or be able to conduct the exact same analysis with a new data set. Give details of randomization and blocking, as well as number of replications, blocks, repeated measurements, samples, or observations. Clearly distinguish between true replications and subsamples within a replication/treatment combination; similarly, distinguish between so-called biological and technical replications. Always specify the experimental design. Identify all the experimental factors being tested, and indicate whether they are being considered fixed or random effects. Except for simple procedures (e.g., t tests, oneway analysis of variance, simple linear regression analysis), cite an appropriate and accessible statistical text and indicate the computer software used. It is not sufficient to just mention the name of a large and multifaceted software package such as SAS, SPSS, or R. List the specific procedure or package used (e.g., PROC MIXED in SAS, or the lme4 package in R). For specialized applications, describe relevant options selected for these procedures. In general, statistical techniques should be described in the Materials and Methods. For complex analyses, it is may be necessary to give snippets of software code in an appendix or in an e-Xtra. Except for calculating means and variances, do not use Excel for statistical analyses (McCullough and Heiser 2008). The statistical methods used for analyzing data should be chosen according to the type of random (response) variable being measured or assessed (e.g., disease incidence, severity, counts, ordinal ratings), and the type of factors being investigated (fixed or random effects, qualitative or quantitative). For continuous response variables, such as yield or disease severity (proportion of the surface area diseased), parametric methods, such as analysis of variance (ANOVA) and linear mixed models, are appropriate, although nonparametric methods can also be used. However, transformations are usually needed for disease severity with parametric analyses, since the variance is a function of the mean. Generalized linear mixed models (GLMMs) with a non-identify link function can also be used. For binary observations, proportions out of a total of n observations (e.g., disease incidence), and counts (e.g., number of lesions or number of spores), parametric methods such as linear mixed models can be used only if the data are properly transformed; preferably, GLMMs can be used with an appropriate selection of the discrete distribution (e.g., binomial, Poisson, negative binomial) and link function. Model fits should be evaluated through the residual plots to confirm that a reasonable model and data transformation (or link function) are being used. Schabenberger and Pierce (2002) provides details on parametric data analyses. When experimental factors are being analyzed as random effects, software designed for mixed models must be used. For ordinal measurements (e.g., disease rating on a 0 to 3 scale designating the ordinal degree of symptoms), nonparametric methods based on ranks should generally be used for hypothesis testing. See Shah and Madden (2004) for recommendations on the treatment of ordinal data. As an alternative, parametric proportional-odds models may be used for ordinal data, if there are sufficiently large numbers of observations for each experimental unit. If ordinal rating scores are used for ranges of disease severity (e.g., Horsfall-Barratt [where, for instance, a “2” corresponds to a severity range from 3 to 6%, and a “3” corresponds to a range of 6 to 12%]), each score should be converted to the midpoint of the corresponding disease severity range prior to use of a parametric analytical technique. Avoid use of arbitrary significance-level (P) cut-offs, such as “significant or not significant at P = 0.05.” Instead, give the achieved significance level for statistical tests (e.g., F was significant at P = 0.025, or P < 0.001 for very small probabilities). In 3 general, do not rely solely on hypothesis tests; rather, estimation of means, medians, mean differences, or other contrasts, with appropriate measure of variability (uncertainty), is preferred. When means (or medians) are followed by ± x, indicate whether x refers to the standard deviation, standard error, or half the confidence interval; error bars in graphs should similarly be defined. For large-scale studies, with hundreds or thousands of treatments, it may not be practical to show all estimated means or contrasts; in this case, presented results could be limited to P values or related summary statistics (this will depend on the study). For Bayesian analysis, always describe the prior distributions chosen for all the parameters (including the variance and covariance parameters) and the distribution (likelihood) used for the response variable. Graphical presentation of posterior distributions for the parameters is recommended. At a minimum, summary statistics for the location and dispersion of the posterior distributions should be given in tabular form. When bootstrapping methods are utilized in analysis, details of the bootstrap method should be given, such as whether parametric or nonparametric techniques are being used. For studies with multiple treatments, avoid blindly utilizing multiple-comparison procedures, where all possible pairwise contrasts of means are determined. In many cases, it is preferable to calculate only the contrasts (e.g., pairwise differences) of means of interest, based on hypotheses developed before the data collection. When multiple comparison procedures are utilized, the specific procedure should be clearly indicated; Duncan’s multiple range test should not be used. When the effects of a quantitative variable (e.g., temperature) are studied, it is preferable to use regression analysis or mixed models with continuous (and qualitative) factors instead of methods developed solely for a nonordered qualitative factor. Wherever possible, researchers should consult with a statistician before designing an experiment and when analyzing the results. Viruses. In formal taxonomic usage, virus family, subfamily, and genus should be capitalized and printed in italics. When used formally, the name of the taxon should precede the term for the taxonomic unit, e.g., the family Bunyaviridae and the genus Tospovirus. Formal use of a virus species name also should be printed in italics, with the first word and any subsequent proper noun capitalized (e.g., Wheat American striate mosaic virus). Generally, the designation of the taxonomic unit “species” need not precede the species name: for example, Tomato spotted wilt virus need not be written as the species Tomato spotted wilt virus. The first use of a virus species name in a paper usually should be formal and, therefore, italicized with the first word capitalized. Subsequent reference to the same virus should be by the accepted acronym, which is not italicized, e.g., TSWV. Virus names written in tables should be written formally. The name of a tentative species whose taxonomic status is uncertain should not be written in italics, but its first word (and any proper nouns) should be capitalized. In informal vernacular use, the virus family, subfamily, and genus should be lowercase and not printed in italics. This generally does not apply to virus species names, because acronyms are applied after the first use, which is formal. The name of the taxon, if used, should follow the term for the taxonomic unit, e.g., the tospovirus genus. When used informally, the name of the taxon should not include the formal suffix, e.g., the bunyavirus family, not the bunyaviridae family. Usually, these constructions should simply be avoided, because they lead to unnecessary ambiguity. Formal taxonomic usage is preferred, particularly when the formal family and genus names have the same root terms, e.g., Bromoviridae and Bromovirus. Quick Guide to APS Editorial Style (see text of Instructions for more complete references) Numbers Numerals for measurements, including ad hoc measurements such as drops, wells. Commas in numbers of 4 digits or more (except for digits used as designations). Zero in front of decimal points. In lists where one item is multidigit, use numerals throughout. Spell out numbers at the beginning of a sentence (if number is spelled out, unit of measure also should be spelled out). -fold: threefold, manyfold, 10-fold. Ranges: use “to” rather than “–,” except in tables. Measurements Do not abbreviate measurements in titles. Time: second (s), minute (min), hour (h), day (day), week, month, year. liter (spell out), but ml, µl, etc. Use the degree symbol with temperature (70°C). Binomials and trinomials All taxa are italicized. In trinomials, always spell out species, e.g., X. campestris pv. campestris (not Xcc). Molecular weight and Daltons Correct: The molecular weight of protein x is 54,000. The molecular mass of protein x is 54,000 Da [or 54 kDa]. Incorrect: The molecular weight of protein x is 54,000 Da [or 54 kDa]. Enumeration Use (i), (ii), (iii), (iv). Prefixes and suffixes Generally should be closed up (e.g., postinfection, loopsful), even in nonstandard constructions; see dictionary or style manuals for exceptions. Compound words When two words are used as adjectives preceding a noun, as a rule they are hyphenated. If uncertain, consult the dictionary (Meriam-Webster) or style manuals. 4 Abbreviations Consult ACS (Dodd 1997) and the short list below for the standard abbreviations for common terms. Authors may coin abbreviations. Limit the use of coined abbreviations to terms used frequently and unusually long terms. Spell out the term and place the abbreviation in parentheses at first use; use the abbreviation after that. Common abbreviations and terms aa—amino acids Carborundum Casamino Acids Celite CFU—colony-forming units, do not spell out if preceded by a numeral cheesecloth chi-square test or χ2 cis, trans-italicize cM—centimorgan, spell out at first use Coomassie brilliant blue df—degrees of freedom, do not spell out if preceded by a numeral et al., not et al. GLM—general linear model gram negative, gram positive Gram stain LB broth—Luria-Bertani broth LR white resin MAb—monoclonal antibody P—probability, do not spell out Parafilm phytoplasma, not MLO or mycoplasmalike organism potato dextrose agar, not potato-dextrose agar ppm—parts per million Rf —retardation factor V8 juice agar General Editorial Style Papers will be rejected outright if the quality of English is insufficient to enable peer review. Most of the style guides mentioned previously have good discussions of English, grammar, and style. Other good general references include The Chicago Manual of Style and Merriam-Webster’s Collegiate Dictionary. Citations. A citation should always be to the original source of publication, whether print or online. Guidelines for citing in text. Use the author-year method of citing publications. For example, “Several studies (Anderson 1994; Jones et al. 1992, 1997a, b; Smith and Roberts 2002) have reported similar findings.” List citations in alphabetical order by authors’ surnames. When citing multiple works by the same author, list articles by one author before those by multiple authors. Determine the sequence by alphabetizing the first author’s surname and subsequent authors’ surnames, by the year of publication (most recent last), and, if necessary, by the page numbers of articles published in the same journal. Guidelines for reference list. List all references in alphabetical order by authors’ surnames. Single-author works should be listed before works with multiple authors. Works by the same author(s) should be ordered chronologically. Italicize Latin binomials, capitalize German nouns, and insert diacritical marks. List specific pages of books. Refer to the Serial Sources for the BIOSIS Database (BIOSIS) for accepted abbreviations of journal names. Do not abbreviate one-word titles of journals. Double-check the accuracy of title abbreviations, page and volume numbers, and dates and check that each reference is cited in text. Only references generally available through libraries and online open-access journals should be listed in Literature Cited. If a work cited is in preparation, submitted but not accepted for publication, or not readily available in libraries, cite the work in parentheses in the text, e.g., (J. Jones, unpublished data) or (J. Jones, personal communication), not in Literature Cited. A copy of the letter from the person supplying unpublished information should be included as an e-mail attachment when submitting a manuscript, or sent to the assigned Senior Editor by fax or mail. To cite an article as in press, you must have a letter of acceptance from a journal or book editor or have a copy of the galley proof for book chapters, bulletins, etc. Avoid excessive reference to unpublished information. Online publications. Beginning in 2005, each article appearing in an APS journal has a unique doi number assigned to it for future reference. It is the policy for APS journals to make changes to an online PDF if an error occurs that requires publication of an erratum for that article. A note will appear at the end of the revised file to describe the change. Fig. 1 Fig. 2 Tables Cite tables in numeric order in the manuscript. Tables should be intelligible without reference to the text or another table. Do not repeat data in the text that are given in a table or figure. The title should summarize the information presented in the table without repeating the subheadings. Subheadings should be brief. Tables are used to present precise numerical data that show comparisons or interrelationships. The minimum number of columns is two. Lists should be incorporated into the text. Long tables including detailed information on isolates used or listing sequence information should be uploaded as supplemental tables. Nonessential details should be omitted or listed as supporting material. Numbers should be rounded to significant digits. Abbreviations are acceptable; explain any nonstandard abbreviations in footnotes. Footnotes are designated with superscript lowercase letters. Figures General. Each submitted illustration should be labeled with the figure number, first author’s surname, and Phytopathology. Captions should describe the contents so each illustration is understandable when considered apart from the text. Cite all figures in numeric order in the manuscript. Numbers and lettering should be in a 10-point sans serif type (Helvetica preferred) and bold; capitalize only the first word and proper nouns in each label. Panel designations—A, B, C, etc. should be uppercase letters in an 18-point sans serif type that matches the font used for the rest of the labeling. Keep font sizes consistent among figures. Figures should be sized to fit one (88 mm) or two columns (183 mm); maximum height is 250 mm, including caption. Line drawings. Affix index marks to ordinates and abscissae. Avoid too bold lettering, numbers, and lines for coordinate axes and curves. Graphs should be “boxed” with tic marks on axes as 5 needed. Use solid black or white or hatch or stripe patterns in bar graphs (Fig. 1); shaded columns do not reproduce well, either becoming indistinguishable or fading to white. Use solid black and white symbols; shaded or screened rules and symbols will not reproduce. Only standard symbols (boxes, circles, triangles) or other typographic elements should be used. If necessary, please provide a key to any symbols as part of the figure (Fig. 2). Only standard symbols can be reproduced in captions. Portions of a composite line drawing or graph should be provided as a single illustration. Digital image file specifications. Digital files must be saved in .tif, .eps, or .jpg format for PC or in .tiff, .pict, .jpeg, or .eps format for Apple. If high-resolution image files cannot be provided in the formats listed above, original image files generated with MS Office programs (such as Word, PowerPoint, or Excel) can be submitted. Image resolution must be at least 360 ppi (600 ppi is preferred for line art and figures with text) at the final printed image size. One-column images must have a minimum of 1,260 pixels in width and two-column images a minimum of 2,610 pixels. If the final printed image size is unknown, size the image at a larger final print size, maintaining the required resolution, and APS will down-sample the image to fit the final print dimensions (to main- tain quality, APS cannot enlarge a digitized image). Black and white images must be saved as grayscale images. Color images should be saved in RGB format. Photographs should be cropped at right angles to show essential details. Scale bars should be inserted to indicate magnification. Images should be clear and of high quality. Color illustrations. The cost of color reproduction must be paid by the authors (for current costs, see the Page Fees section). If you have questions regarding figures, please contact Patti Ek at [email protected] or view Phytopathology figure instructions online. Illustrations (photographs, drawings, or diagrams) from an article or related to an article may be submitted for consideration for the journal cover. Such figures should be submitted through Manuscript Central as Supplemental material and labeled as cover art followed by a caption. Guidelines for electronic manuscript submission Phytopathology requires that all manuscripts be submitted electronically via an Internet service called Manuscript Central in order to be considered for publication. Electronic submission speeds the handling of your manuscript and allows you to monitor its status at any time during the review process. Checklist for papers submitted to Phytopathology Authors can expedite processing of their manuscripts by following the formatting guidelines outlined below in preparing their manuscripts for submission to Phytopathology. Content Significance and originality of work are shown. Reproducibility of results is illustrated. Objectives are clearly stated in the introduction. Introduction includes a succinct evaluation of the topic, including all relevant literature citations. Experimental design and methodology are fully explained. Proper and sufficient analyses have been conducted (review by qualified statistician before submission is encouraged). Discussion relates work to other published material and addresses strengths and weaknesses of research. Major conclusions are supported by results from repeated experiments. Manuscript has been reviewed critically before submission. Format With line numbers and double-spaced (including tables and figure captions). Typescript is 12 point. First author’s name, page number, and Phytopathology in upper right corner of each page. Tables on numbered pages after Literature Cited section and in text format. Captions for figures, including e-Xtra figure captions, on a separate numbered page following the tables. Also include captions preceding each figure for easy reference. Section heads provided. Title does not exceed 100 characters and spaces. (Do not include a pathogen name in parentheses after a disease name unless essential for clarity. Do not use both common and scientific names for organisms in the title.) Author name(s) listed under the title. Affiliations, addresses, and acceptance date are given in separate paragraphs beneath the author’s name. Acknowledgments are at the end of the text. Corresponding author’s name and e-mail address are given before the abstract. 6 Reference line is provided (authors, year, title, journal) under abstract head. Abstracts are mandatory and limited to one 200 word paragraph. Organization of text Major sections after the introduction are Materials and Methods, Results, Discussion, and Literature Cited. General techniques and methods are described in Materials and Methods; brief descriptions of experiments and trials are given in Results. (Subheadings may be used, but avoid excessive fragmentation of the text.) Footnotes to the text are not permitted. Please refer to published articles for standard formatting of heads and subheads. Published references are listed in Literature Cited. References are in alphabetic order by authors’ surnames. Citations in text are cited by the author-year method. Digital images have been checked for adequate resolution. Figures are boxed. The first letter of the first word of each label is capitalized; all others are lowercase, except proper nouns. Figures are prepared for same-size reproduction (88 or 183 mm wide; maximum 250 mm deep, including caption). Consistent font style and sizing is used for all figures. Supporting material Copy of first page or letter of acceptance is provided for all in press citations. (The Senior Editor may request that preprints be provided.) Copies of personal communication verification are provided. Permission has been granted for copyrighted material. Accession numbers obtained for nucleotide and/or amino acid sequences are provided as a footnote to the first page or in text. Voucher cultures and specimens have been deposited in recognized collections. Illustrations (photographs, drawings, or diagram) from an article or related to an article may be submitted for consideration for the journal cover. Such figures should be labeled and include a caption. Procedure. Type http://mc.manuscriptcentral.com/Phytopathology in your Internet browser to bring up the log-in screen. First-time users must create an account. Follow the on-screen directions to create your account and submit your manuscript. Text files can be in Word or Rich Text. Figures should be submitted in .tif, .jpg, Powerpoint, or Excel formats. Technical problems. Assistance with technical difficulties in submission is available from ScholarOne, Inc., the parent company of Manuscript Central. 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Papers that appear as “First Look” articles may be rapidly indexed by PubMed, Scopus, ISI Web of Science, and other online databases with links back to the “First Look” article, before the formatted and edited papers appear in the online issue. The following statement will appear on papers posted in First Look: Phytopathology “First Look” paper • http://dx.doi.org/10.1094/ PHYTO-00-00-0000 • posted 00/00/2015 This paper has been peer-reviewed and accepted for publication but has not yet been copy-edited or proofread. The final published version may differ. For more information, contact the Editor-in-Chief: Krishna V. Subbarao E-mail: [email protected] Or the Technical Editor: Kristen Barlage E-mail: [email protected] Biosecurity Statement The APS biosecurity policy covers details for screening for research that may constitute misuse of plant pathological methods or potential danger from the improper application of knowledge. In addition, before a report on a discovery of an Agricultural Select Agent can be submitted for publication, the detection of the Select Agent must be reported to USDA APHIS. See the APS Select Agent policy. LITERATURE CITED American Phytopathological Society Committee on the Standardization of Common Names for Plant Diseases. 2010. Common Names of Plant Diseases. Published online by The American Phytopathological Society, St. Paul, MN. American Society of Agronomy (ASA), Crop Science Society of America (CSSA), and Soil Science Society of America (SSSA). 1998. Publications Handbook and Style Manual. ASA, CSSA, and SSSA, Madison, WI. Anonymous. (Current) Farm Chemicals Handbook. Meister Publishing Co., Willoughby, OH. BIOSIS. (Current) Serial Sources for the BIOSIS Database. BIOSIS, Philadelphia, PA. Bosik, J. J., ed. 1997. Common Names of Insects and Related Organisms, 4th ed. Entomological Society of America, Lanham, MD. CBE Style Manual Committee. 1994. Scientific Style and Format: The CBE Manual for Authors, Editors, and Publishers, 6th ed. Cambridge University Press, Cambridge. Dodd, J. S., ed. 1997. The ACS Style Guide: A Manual for Authors and Editors. American Chemical Society, Washington, DC. Environmental Protection Agency (EPA), Pesticide Regulation Division. (Current) Acceptable Common Names and Chemical Names for the Ingredient Statement on Pesticide Labels. EPA, Washington, DC. Farr, D. F., Bills, G. F., Chamuris, G. P., and Rossman, A. Y. 1989. Fungi on Plants and Plant Products in the United States. American Phytopathological Society, St. Paul, MN. Garrity, G. M., and Boone, D. R., eds. 2001. Bergey’s Manual of Systemic Bacteriology, Vol. 1, 2nd ed. Springer Verlag, New York. International Union of Biochemistry and Molecular Biology. 1992. Enzyme Nomenclature 1992. Academic Press, San Diego, CA. King, R. C., Stansfield, W. D., and Mulligan, P. K. 2006. A Dictionary of Genetics, 7th ed. Oxford University Press, New York. Kirk, P. M., Cannon, P. F., David, J. C, and Stalpers, J., eds. 2008. Dictionary of the Fungi, 10th ed. CAB International, Wallingford, U.K. Lewis, R. J., Sr. 2007. Hawley’s Condensed Chemical Dictionary, 15th ed. John Wiley and Sons, Chichester, U.K. Mabberley, D. J. 1997. The Plant-Book: A Portable Dictionary of the Vascular Plant, 2nd ed. Cambridge University Press, Cambridge. McCullough, B. D., and Heiser, D. A. 2008. On the accuracy of statistical procedures in Microsoft Excel 2007. Comput. Stat. Data Anal. 52:4570-4578. O’Neil, M. J., ed. 2006. The Merck Index: An Encyclopedia of Chemicals, Drugs, and Biologicals, 14th ed. Merck & Co., Whitehouse Station, NJ. Rieger, R., Michaelis, A., and Green, M. M. 1991. Glossary of Genetics: Classical and Molecular, 5th ed. Springer Verlag, New York. Schabenberger, O., and Pierce, F. J. 2002. Contemporary Statistical Models for the Plant and Soil Sciences. CRC Press, Boca Raton, FL. Shah, D., and Madden, L. V. 2004. Nonparametric analysis of ordinal data in designed factorial experiments. Phytopathology 94:33-43. Skerman, V. B. D., McGowan, V., and Sneath, P. H. A., eds. 1989. Approved Lists of Bacterial Names, Amended ed. American Society for Microbiology, Washington, DC. Stenesh, J. 1989. Dictionary of Biochemistry and Molecular Biology, 2nd ed. Wiley Interscience, New York. Terrell, E. E., Hill, S. R., Wiersema, J. H., and Rice, W. E. 1986. A Checklist of Names of 3,000 Vascular Plants of Economic Importance. USDA Handb. 505, Washington, D.C. 7
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