BD Horizon™ V500-C Antibody Conjugates
Violet Laser Reagents
Features
ptimized for compensation setup using
O
BD™ Multicolor CompBeads
educes spillover into the FITC channel compared to
R
AmCyan
aximizes choice and flexibility for multicolor panel
M
design
Available as CE-IVD and RUO(GMP) reagents
200
250
S upports 8-color analysis on the BD FACSCanto™ II
flow cytometer
(x 1,000)
BD Biosciences continues to expand the options for multicolor
flow panel design with the development of an optimized
BD Horizon™ brand dye for the violet laser. The BD Horizon
V500-C dye has been specifically engineered to reduce spectral
overlap into the FITC channel (Table 1) and to be compatible
with BD™ Multicolor CompBeads particles for establishing
compensation values (Table 2). BD Horizon V500-C is an organic
dye that offers the same stability and compatibility typical of the
BD Horizon family of dyes.
Optimized for the violet laser
BD Horizon V500-C is a completely novel organic dye structure
that has been specifically engineered to have optimized spectral
characteristics for use with the violet laser. It is optimally suited
for use with BD FACS™ brand flow cytometers equipped with
violet lasers such as the BD FACSCanto II flow cytometer. When
used with these instruments, BD Horizon V500-C reagents
demonstrate improved spectral characteristics compared to other
violet laser excitable dyes, and enables advanced multicolor
panels together with the BD Horizon V450 reagents.
50
100
SSC
150
With a maximum excitation at 415 nm and an emission peak
at 500 nm, BD Horizon V500-C is readily compatible with the
standard filter sets on BD FACS brand flow cytometers equipped
with violet lasers. The addition of BD Horizon V500-C increases
fluorochrome choice by enabling 8-color analysis on the
BD FACSCanto II flow cytometer.
Lymphocytes
0101
102
103
104
105
105
CD45 V500-C
Expand your multicolor capability
Designed with your current protocols in mind, BD Horizon
V500-C reagents demonstrate compatibility with the standard
staining buffers typically used in flow cytometry. Compatibility
with red cell lysis buffers such as BD FACS™ lysing solution as
well as permeabilization buffers for intracellular staining (eg,
BD FACS™ Permeabilizing Solution 2 and BD Intrasure™) has
been shown. BD Horizon V500-C shows stability in the presence
of paraformaldehyde-based fixatives and its 5 μL/test formulation
simplifies creation of multicolor cocktails.
0
102
103
CD4 FITC
104
Visit bdbiosciences.com for more information.
-102
0
102
103
104
105
CD3 APC-H7
Figure 1. Lysed whole blood gated on CD45 lymphocytes and subsequent
resolution of T cell subsets.
BD Horizon™ V500-C Antibody Conjugates Violet Laser Reagents
Instrument
Excitation Laser Line (nm) Fluorescence Channel
BD FACSCanto II
488
633
405
Fluorochromes provided by BD Biosciences
Green
Yellow
FITC
PE
Red
PerCP
Infrared
PE-Cy™7
Red
APC
Infrared
APC-H7
APC-Cy7*
Green
Blue
BD Horizon V500-C
BD Horizon V450
BD Horizon V500
PerCP-Cy™5.5
Figure 2. BD Biosciences Fluorochrome Reference Chart
To select the optimal combination of fluorochromes, visit bdbiosciences.com/spectra to use the interactive fluorescence spectrum tool.
V500-C SOVs into…
FITC
Beads
% Spillover into FITC
Description
V500-C
AmCyan
CD3
8.6%
43.4%
CD45
8.0%
44.7%
Table 1. BD Horizon V500-C conjugates have significantly less spillover into
the FITC channel compared to AmCyan.
Comparison of lysed whole blood stained with anti-CD3 or anti-CD45 to establish
SOV on a BD FACSCanto™ II flow cytometer.
V450
Absolute
Cells
Difference Beads
(Beads - Cells)
Cells
Absolute
Difference
(Beads - Cells)
Average
8.1
8.3
0.4
10.7
11.9
1.2
Average Max-Min/
Reagent
0.5
0.2
0.3
0.6
0.6
0.3
Total Max-Min
All Lots
1.1
1.2
0.6
1.2
1.2
1.0
Table 2. SOVs for V500-C can be obtained with BD CompBeads or cells.
The average is the value across 18 independent lots of V500-C reagents (three lots
each of six different reagents) measured using BD CompBeads or cellular controls.
The average Max-Min/Reagent illustrates the average variation (difference in the
maximum and minimum SOV) obtained within the three lots for each reagent. The
total Max-Min (all lots) represents the total variation (difference in the maximum
and minimum SOV) obtained across all 18 lots.
BD Horizon™ V500-C CE-IVD(1) Reagents
Description
React.
Clone
Isotype
Size
Reg. Status
Cat. No.
CD3
Hu
SK7
Ms IgG1
100 tests
CE-IVD
652896
CD45
Hu
2D1
Ms IgG1
100 tests
CE-IVD
655873
Ordering Information
BD Horizon™ V500-C RUO(GMP)(2) Reagents
Description
React.
Clone
Isotype
Size
Reg. Status
Cat. No.
CD3
Hu
SK7
Ms IgG1, k
100 tests
RUO (GMP)
647453
CD4
Hu
SK3
Ms IgG1, k
100 tests
RUO (GMP)
647455
CD8
Hu
SK1
Ms IgG1, k
100 tests
RUO (GMP)
647457
CD14
Hu
MfP9
Ms IgG2b, k
100 tests
RUO (GMP)
647459
CD20
Hu
L27
Ms IgG1, k
100 tests
RUO (GMP)
647463
CD45
Hu
2D1
Ms IgG1, k
100 tests
RUO (GMP)
647449
CD138
Hu
MI15
Ms IgG1, k
100 tests
RUO (GMP)
650659
X40
Ms IgG1, k
100 tests
RUO (GMP)
647451
Mouse IgG1
CE-IVD: For in vitro diagnostic use. CE marked in conformity with the European IVD Directive 98/79/EC
(Directive on in vitro diagnostic medical devices).
(1)
RUO(GMP): For Research Use Only (General Manufacturing Practices). Not for use in diagnostic or therapeutic procedures.
(2)
*APC-Cy7: US Patent 5,714,386
Cy™ is a trademark of Amersham Biosciences Corp. Cy™ dyes are subject to proprietary rights of Amersham Biosciences Corp
and Carnegie Mellon University and are made and sold under license from Amersham Biosciences Corp only for research and in
vitro diagnostic use. Any other use requires a commercial sublicense from Amersham Biosciences Corp, 800 Centennial Avenue,
Piscataway, NJ 08855-1327, USA.
BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2012 BD
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