A Newsletter from Peptides International
Volume 25, No. 1, 2012
Peptisyntha and PI Form Global Partnership!
Peptisyntha and Peptides International have entered into a
global partnership for the production of research grade peptide APIs (active pharmaceutical ingredients).
The announcement was
made at the recent TIDES
conference in Las Vegas,
Nevada. As a result, customers will benefit from
access to the combined
research and manufacturing expertise of both companies as well as seamless transition from research level through commercial manufacturing.
Each company brings distinct competencies and experience
to the partnership. PI produces custom peptides across a
wide range of technologies from multi-disulfide peptides
to multi-step organic synthesis. It also offers an extensive
portfolio of building blocks and other peptide synthesis
tools. Peptisyntha, in turn, has developed and demonstrated
over the years a unique set of expertise in the design of cost
effective peptide manufacturing processes and in the cGMP
production of clinical and commercial peptides API’s. It’s
facilities have had numerous successful FDA and EMEA
inspections and offer a full range of solid phase, liquid,
phase, and hybrid synthesis capabilities.
Multi Media Matters
Each year Genetic Engineering News (GEN) publishes a
review of the peptide industry in its July 1st issue. This
year’s edition includes comments from PI’s President,
Dr. Michael Pennington on the current and future challenges of peptide synthesis. In addition, the ad reproduced below appeared in the same issue to illustrate the
significance of the new PI-Peptisyntha partnership.
Let us guide you to the goal.
A joint press release issued on May 21st included the following comments:
Dr. Johan Devenyns, Managing
Director of Peptisyntha stated:
“Providing project continuity at
all scales will save our customers
time and resources while benefitting
from our manufacturing expertise
and excellent services.”
Large HPLC column for cGMP,
located at Peptisyntha S.A.
(Brussels, Belgium)
Dr. Bryce Johnson, Chief Executive
Officer of Peptides International
added: “This partnership represents
the combination of over fifty years
of peptide experience by two companies who share the same commitment to the highest standards of
quality and operational excellence.”
There can be lots of dead ends,
blind alleys, and detours on the
road from R&D to cGMP. Finding expertise that can help you
avoid these wasted efforts while
supplying quality materials can
be difficult too.
But now there is a solution:
Peptides International and
Peptisyntha have partnered
to create a seamless stream
of technology and expertise,
allowing you to focus on the
project, not project management.
The route to success.
There's Lots More BIG News Inside: Certification to ISO 9001! A New Catalog! A New Sales Manager! And More...
PEPNET.COM
+1 502 266 8787
1 800 777 4779 USA and Canada
Peptisyntha.com
+1 310 782 7524 USA
+ 32 2264 2245 Belgium
Product Quality and Value • Strict Confidentiality • Proactive Project Management • Friendly Service
PI Now Certified to ISO 9001:2008!
Dr. Patricia Coxon
QA Manager
Peptides International recently announced
that its production and distribution have been
certified to the ISO 9001:2008, International
Quality System Standard. These standards
are recognized globally as the framework
for well-managed, customer-focused business systems with strong emphasis on quality management, continual improvement,
achievement of business results and customer satisfaction.
The certification process
“The ISO certification
involved intensive internal
reflects
the hard work,
preparation during which
dedication,
and excellence
the company documented
our
staff
commits
daily
and standardized the proin order to deliver
cesses and procedures of its
quality products to our
operations from customer
inquiry through final shipcustomers.”
ment. Objectives and met- PI's QA Manager
rics were determined to
Dr. Patricia Coxon
monitor performance and
employees were trained in ISO standards. Following a series
of internal audits, the company’s quality system was audited
and certified by Smithers Quality Assessments Inc. (SQA),
a member of the Independent Association of Accredited
Registrars. The entire certification process was managed
by Peptides International’s Quality Assurance Manager, Dr.
Patricia Coxon.
He Would Love to Quote You!
New Products from the Peptide Institute!
Since the last edition of PEPNET (Vol. 24, No. 2) the Peptide
Institute has introduced products in several key research
areas. These products can be ordered directly from our
website catalog:
CODE
PRODUCT
QTY
USD
AGP-4469-s AG30/5C (0.1 mg vial)
Antimicrobial Peptide with
Angiogenic Properties
0.1 mg
vial
110
AMP-4468-s AMP-IBP5 (Human)
Insulin-Like Growth Factor-Binding
Protein 5; Antimicrobial Peptide
0.1 mg
vial
110
1 mg
vial
330
TNN-4464-v
[D-Leu7]-(-)-Ternatin
PLP-3404-v
4 [D10]Leu Insulin (Human)
Stable Isotope-Labeled Insulin
Useful for Standardization of Insulin
Immunoassays
0.02 mg
vial
220
SNE-3230-v
Abz-Ala-Pro-Glu-Glu-Ile-MetArg-Arg-Gln-EDDnp
Fluorescence-Quenching Substrate
for Human Neutrophil Elastase
1 mg
vial
110
SNP-3232-v
Abz-Val-Ala-Asp-Nva-Arg-AspArg-Gln-EDDnp
Fluorescence-Quenching Substrate
for Human Neutrophil Proteinase 3
1 mg
vial
110
PKC-3403-v
Myr-Ser-Ile-Tyr-Arg-Arg-Gly-AlaArg-Arg-Trp Arg Lys Leu
Myristoylated Cell Permeable PKCζ
Pseudosubstrate Inhibitor
0.5 mg
vial
110
Peptides International has a new Sales Manager. Sharp readers may recognize Tim Morgan's name or face
from previous articles. Tim, a Louisville native who joined the company in 2007, holds a BS in Biology and
an MA in Political Science (International Business) from the University of Louisville. His most recent position
at PI was a research chemist specializing in custom peptide synthesis.
Tim Morgan
Sales Manager
Tim’s technical knowledge and broad background in our laboratory, together with his strong communications
skills and business training, will allow him to quickly coordinate and respond to requests for quotations
product availability, and other reseach needs. Tim will lead PI’s Louisville-based sales efforts with responsibility for management of strategic sales initiatives, key accounts, and custom and catalog quotations. If you
would like to contact Tim to discuss how PI can assist with your research needs, he can be reached by email
at [email protected] or by calling Peptides International directly.
Employee
Spotlight
Peptides International is proud of the diversity of its employees, while sharing a common desire
to offer our customers their absolute best in creating service and product value. Here's someone
who has seen - and has had a vital role in - the company's growth.
Anyone who has contacted PI over the years, whether by email, fax, phone call, or website request, has most
likely had a reply from Melissa Hundley. Melissa is one of the the longest-serving Peptides International
employees, and as such has seen the many changes that have come about in the seventeen years since she
joined the company in 1995. She has proved to be a loyal, dedicated, and trusted employee and initmately
knows all aspects of the business and customer service departments, with her main focus being accounting.
Along with all the changes that Melissa has seen at Peptides International are the ones that have occurred
outside of work: in that time, she has married, and she and her husband (a professional firefighter) are
Melissa Hundley
raising two children. She is very involved in her family's activities, working as a Volunteer Support Team
Leader at the fire department, and with her children's sports. She enjoys traveling, dining out and spending
time with her family. We are grateful for Melissa's dedication over the years - here's to seventeen more!
Research Focus: What are Disulfide Rich Peptides?
Nature has afforded a means of making highly potent biologically active peptides and mini-proteins by utilizing stabilizing elements to lock the pharmacophore of the molecule into a desired
configuration. (The pharamcophore describes the molecular
features which are necessary for molecular recognition of a ligand
by a biological macromolecule). Peptides which are often isolated from venomous organisms (cone snails, snakes, sea anemones, scorpions,
spiders, etc.) utilize multiple disulfide bonds to lock them into their biologically
active form. Thus, multiple disulfide bonds are a common structural scaffold
used to stabilize a pharmacophore. The activities of these venom-derived
These most recent additions, Hepcidin (Mouse) and
peptides often are highly potent ion channel blockers, protease inhibitors and (Rat), bring the product line to nearly a dozen offerings.
antimicrobial molecules. More recently, disulfide rich peptides have also been
found in non-venomous animals. Interestingly, some of these molecules have homologs that are often found in venoms.
These molecules include disulfide rich peptides such as defensins (antimicrobial) and hepcidins (iron regulatory hormone).
Peptides International offers a wide assortment of these ion channel toxins, defensins, hepcidins, and integrin inhibitors to
aid research in this interesting area. Several are featured below.
New Tools for Hepcidin Research
Hepcidin was initially isolated as a liver-expressed antimicrobial peptide in both blood and urine. The function
of the peptide was further elaborated as a key hormonal
regulator of iron metabolism in vertebrate species. From
an evolutionary standpoint, the peptide sequence is highly
conserved from fish species up to humans. The active form
of the peptide is a 25 residue molecule which is stabilized by
four intramolecular disulfide bonds. Research on hepcidin
and its key role in iron regulation has prompted a great deal
of interest in both the academic and pharmaceutical arenas.
Mass-spec based assays utilize stable isotope derivatives
for determination of hepcidin levels in plasma and urine
samples. The availability of hepcidins from standard laboratory animals has become critical with the development of
therapeutics for treatment of hemochromatosis and certain
inflammatory conditions. Traditionally, hepcidin research in
mouse and rat models for Fe transport has been limited by a
lack of availability of larger quantities. With the aid of new
production technologies, Peptides International is pleased
to offer new 1 and 5 mg sizes of these two high purity
hepcidins. Human and baboon hepcidins, as well as analogs
with C-13 labels for mass spec assays, are also available in
our portfolio.
CODE
PRODUCTS
PLP-4467-v
Hepcidin (Rat)
PLP-3769-PI
Hepcidin (Rat)
H-Asp-Thr-Asn-Phe-Pro-Ile-Cys-LeuPhe-Cys-Cys-Lys-Cys-Cys-Lys-AsnSer-Ser-Cys-Gly-Leu-Cys-Cys-Ile-Thr
(Disulfide bonds between Cys7-Cys23,
Cys10-Cys13, Cys11-Cys19, Cys14-Cys22)
Iron-Regulatory Hormone
PLP-4434-s
Hepcidin / LEAP-1 (Mouse)
PLP-3773-PI
Hepcidin / LEAP-1 (Mouse)
Asp-Thr-Asn-Phe-Pro-Ile-Cys-IlePhe-Cys-Cys-Lys-Cys-Cys-Asn-AsnSer-Gln-Cys-Gly-Ile-Cys-Cys-Lys-Thr
(Disulfide bonds undetermined)
Iron-Regulatory Hormone
QTY
USD
0.5 µg
vial
365
1 mg
5 mg
1500
6750
0.1 mg
vial
260
1 mg
5 mg
1500
6750
Heat Stable Enterotoxins for Bowel Treatments
Recently two therapeutic candidates have entered clinical
trials for treating chronic constipation and irritable bowel
syndrome. The peptide Linaclotide is an analog of heat
stable enterotoxin isolated from E. coli. The second peptide,
Plecantide, is an analog of uroguanylin. Both of these molecules utilize the same mechanism of action to stimulate H2O
secretion into the lumen of the bowel and thereby facilitate
transit by acting as a guanylate cyclase type-C receptor agonist. Peptides International is pleased to offer both of the
parent peptides: uroguanylin, which is stabilized two disulfide bonds (both the α and β topoisomers are available), and
heat stable enterotoxin, which is stabilized by three disulfide
bonds.
CODE
PRODUCTS
QTY
USD
ENT-3744-PI
Enterotoxin STp
H-Asn-Thr-Phe-Tyr-Cys-Cys-GluLeu-Cys-Cys-Asn-Pro-Ala-CysAla-Gly-Cys-Tyr-OH
(Disulfide bonds between Cys5
and Cys10, Cys6 and Cys14; Cys9
and Cys17)
E.coli Enterotoxin
1 mg
5 mg
625
2500
PUG-4295-s
Uroguanylin Isomer A (Human)
Asn-Asp-Asp-Cys-Glu-LeuCys-Val-Asn-Val-Ala-CysThr-Gly-Cys-Leu
(Disulfide bonds between
Cys4-Cys12 and Cys7-Cys15)
Guanylate Cyclase C Activator
0.1 mg
vial
135
PUG-4463-s
Uroguanylin Isomer B (Human)
Asn-Asp-Asp-Cys-Glu-LeuCys-Val-Asn-Val-Ala-CysThr-Gly-Cys-Leu
(Disulfide bonds between Cys4Cys12 and Cys7-Cys15)
Natriuretic Factor
0.1 mg
vial
105
For many more disulfide rich peptides, including a large selection of toxins, please visit our website, PEPNET.COM.
PEPNET.COM → New Products
PEPNET.COM → Product Brochures
PRESORTED
STANDARD
U.S. Postage
PAID
PEPTIDES
INTERNATIONAL
P.O. Box 99703
Louisville, KY 40269-0703
Address Service Requested
In This Issue
•
Global cGMP Partnership
•
Certification to ISO 9001:2008!
•
Disulfide Rich Peptides
•
Hepcidins
•
Heat Stable Enterotoxins
•
Employee Spotlight
•
and lots more!
The NEW Catalog is Here!
New Edition, New Formats: Peptides
International's most recent product
catalog has been released. With over
280 products added since the last
edition, it is our biggest, most comprehensive catalog yet. In addition to
the traditional print version, there are
now four other formats available: USB
memory stick, CD, and PDF (emailed
or online). If you haven't been in
contact with PI recently and would like
a copy, please check with us today and
let us know which version you would
like to receive.
This issue of PEPNET is full with lots of exciting new developments
and offerings at Peptides International. The months since the last
issue of the newsletter have seen the rollout lots of new products
(some are featured within this issue), along with a partnership, and
certification. We hope you enjoy perusing this PEPNET edition.
We look forward to hearing from you!
Coming Soon! Another look at toxins and other disulfide rich
peptides, enzyme inhibitors & substrates and much, much more…
Research Focus: Disulfide Rich Peptides
Platlet Aggregation Inhibitors
Echistatin, a polypeptide from the venom of the saw-scaled
viper, Echis carinatus, contains 49 amino acids and 4 disulfide
bridges. The sequence Arg24-Gly-Asp26 of echistatin occurs
in adhesive glycoproteins that bind to the platelet fibrinogen
receptor, a heterodimeric complex composed of glycoproteins IIb and IIIa. Echistain inhibits fibrinogen-dependent
platelet aggregation stimulated by ADP with IC50 = 3.3 x
10-8 M and also prevents aggregation initiated by thrombin,
epinephrine, collagen, or platelet-activating factor.
CODE
ECT-3760-PI
The Benefits of Being Sociable:
Peptides International's social network pages are an upto-the-minute resource where you'll find all the latest
announcements, offers, videos and events related to Peptides
International and the field of peptide chemistry.
Peptides International, Inc.
P.O. Box 99703
Louisville, KY 40269-0703 USA
PRODUCT
Echistatin
H-Glu-Cys-Glu-Ser-Gly-Pro-CysCys-Arg-Asn-Cys-Lys-Phe-LeuLys-Glu-Gly-Thr-Ile-Cys-Lys-ArgAla-Arg-Gly-Asp-Asp-Met-AspAsp-Tyr-Cys-Asn-Gly-Lys-ThrCys-Asp-Cys-Pro-Arg-Asn-ProHis-Lys-Gly-Pro-Ala-Thr-OH
(Disulfide bonds between Cys2Cys11, Cys7-Cys32, Cys8-Cys37,
and Cys20-Cys39)
Phone: 502-266-8787
Fax: 502-267-1329
QTY
USD
1 mg
5 mg
1200
5000
1-800-777-4779
E-mail: [email protected]