Separation Technologies: our work – our life – our passion. ZEOsphere® Chromatography Gels Separation Solutions · Large Scale Batches · Professional Support from Concept to Fullscale · Customized Derivatization ZEOsphere® - High Performance Separation C-Gels High Quality Swiss Production Zeochem AG, a manufacturer of high quality spherical and irregular silica gels and molecular sieves, was established more than 190 years ago and is still based at the original location on Lake Zurich, Switzerland. Zeochem is a subsidiary of the Swiss CPH Chemie+Papier Holding AG. SEM of ZEOsphere® 100 15 µm With ZEOsphere®, a series of spherical silica gels, we are the only Swiss company offering these products. Additionally, Zeochem is one of the few worldwide fully back-integrated silica producers, controlling the quality of the product right from the high purity water glass up to the fully end-capped bonded high performance gel, available in both normal and reversed phase. ZEOsphere® silica gels are produced by a large batch scale emulsion process providing reproducible chromatographic separations of high quality. The efficiency of liquid chromatography relies on narrow particle size distributions, very high mechanical stability and a high specific surface area to deliver consistent and reliable results. We are constantly working on improving this process with one main goal: Absolute uniformity between batches. ZEOsphere® - Technical Information Properties of ZEOsphere® compared to Competitors ZEOsphere® Competitor 1 Competitor 2 Specific surface area [m2/g] Property 303 339 291 Pore volume [ml/g] 0.84 0.90 0.75 Pore size [Å] 110 107 103 d(50) [μm] 15.4 15.0 15.0 d(90) / d(10) [-] 1.61 1.58 1.66 Residual water content [wt%] 0.9 3.1 2.0 pH [-] 5.4 5.0 5.7 Electric conductivity [μS/cm] 3 15 3 Na[ppm] 31 34 110 Fe[ppm] <1 2 <1 Al[ppm] 3 2 8 K[ppm] 5 3 4 Ca[ppm] 39 38 36 Mg[ppm] 7 8 8 Pb[ppm] < 0.1 < 0.1 < 0.1 85 87 166 Total metal content [ppm] Based on Properties of ZEOsphere® 100 15 µm ZEOsphere® C18 Competitor 1 Competitor 2 d(50) [μm] Property 15.4 15.4 14.1 d(90) / d(10) [-] 1.61 1.54 1.51 Carbon content [wt%] 20.3 19.9 15.5 Based on Properties of ZEOsphere® 100 C18 15 µm Spherical Precision SEM of ZEOsphere® 100 15 µm The present product range offers pore sizes from 70 to 100 Å and particle sizes from 5 μm up to 40 μm. Other speciality products can be manufactured according to customers‘ specific requirements. Our surface modification technology enables the bonding of all existing silica gel products regardless of shape, pore and particle size. With our products there are no headaches concerning the scale of your process; the quality of ZEOsphere® ensures seamless transitions from analytical, semi-prep and prep to large scale industrial chromatography, as we are providing the same silica specifications with appropriate particle size distributions. ZEOsphere® can be delivered in 2.5 l polyethylene bottles or 25 kg resealable plastic drums. Test Data Antidepressants Separation Insulin, Desamido Insulin Separation 180 65 160 ZEOsphere® C18 Competitor 1 45 120 UV Signal at 215 nm UV Signal at 254 nm 140 ZEOsphere® C18 Competitor 1 55 100 80 60 35 25 15 40 5 20 -5 0 10 20 30 40 50 Time [min] Test molecules: nortriptyline, amitriptyline, imipramine; mobile phase: MeOH: 25 mM (NH4)H2PO4, 72:28 (w:w), pH=6.3; temperature: 25°C. 0 10 20 30 40 Time [min] Test molecules: insulin, desamido insulin; mobile phase: EtOH: 50 mM (NH4)2SO4, pH 3.5, 28.3:71.7 (w:w); temperature: 25°C. 50 Raw Peptide Mixture 195 ZEOsphere® C18 Competitor 1 175 UV Signal at 220 nm 155 30 ZEOsphere® C18 Competitor 1 25 20 135 15 115 10 95 5 75 0 55 -5 15 35 20 15 -5 0 10 20 30 Time [min] 40 50 60 25 Time [min] 30 35 40 Test mixture: raw 3kDa peptide; buffer A: ACN : 50 mM NaH2PO4: H3PO4, 10.4:89.4:0.2 (w:w:w), buffer B: ACN : 50 mM NaH2PO4: H3PO4, 52.2:47.6:0.2 (w:w:w), gradient from 1% buffer B to 100% buffer B in 30 minutes; temperature: 25°C. Advantages Availability of standard modifications from stock Large batch sizes (180 kg / batch) High carbon load reproducibility Monofunctional bonding technology Standard particle sizes from 5 – 40 μm (further sizes available upon request) C18, C8, NH², CN, SH, Diol, SAX (further derivatizations available upon request) The base material control combined with the proprietary bonding technique make ZEOsphere® the material of choice for all separation processes where high resolution, good peak shape, short elution time are demanded, even at elevated pH values. Major Applications Peptides API Small Proteins Insulin Natural Products Antidepressants Vitamins Amines Steroids ZEOsphere® - Comprehensive Solutions ZEOsphere® displays very uniform perfectly spherical particles, which provide an extremely pressure stable silica gel, free of fines, allowing reproducible column packing with optimized backpressure. The low metal ion content of ZEOsphere® is achieved during our multistep production process. Consequently, metal ion solute interactions are minimized providing symmetrical peaks with little or no tailing. The homogenous surface properties ensure the highest performance with even the most challenging substrates. Cost is a major driver for all pharmaceutical and chemical companies. The purification stage influences the total separation cost significantly. The efficiency and cost of the silica gel is in the focus of today‘s R & D departments and Zeochem offers premium products with competitive prices, supporting our customers by reducing their spend and increasing their yields. Technical Support Zeochem prides itself in providing a second to none service to our customers. Our chemical specialists and the technical sales team utilize an in-house developed software based modelling tool to design the optimum process prior to upscaling from the lab. Often a short discussion with our team is sufficient to provide a comprehensive solution to our customer base. www.zeochem.com Zeochem AG Seestrasse 108 · 8707 Uetikon am See · Switzerland Phone +41 44 922 93 93 · Fax +41 44 920 20 93 [email protected] · www.zeochem.com Zeochem L.L.C. 1600 West Hill Street · Louisville, KY 40210 · USA Phone +1 502 634 7600 · Fax +1 502 634 8133 [email protected] · www.zeochem.com For your local agent or distributor please go to www.zeochem.com A Company of Chemie + Papier Holding AG