BD Vacutainer ® BD Diagnostics - Preanalytical Systems Tube Guide & Recommended Order of Draw* * Clinical and Laboratory Standards Institute (Formerly NCCLS) Guidelines H3-A6, 6th Edition St Vincents University Hospital, Dublin Blood samples should be taken in the following order: Cap Colour Cat. No. Additive Determinations Special instructions Blood Culture If insufficient blood for both culture bottles, use aerobic bottle only. Using a needle-protected butterfly needle collect aerobic (green) bottle first and then the anaerobic (purple) bottle. If using a syringe then the anaerobic bottle is taken first and then the aerobic bottle. Blood Culture bottles should be available on all wards. A supply is available from Microbiology Reception (Mon – Fri) Sodium Citrate Coagulation Testing, PT, INR, APTT, D-Dimer, Thrombophilia Screens, Factor Assays 3-4 Serum LDH, NT-pro BNP, Drugs (Lithium, Phenytoin, Theophylline, Methotrexate, Valproate, Carbamazepine, Paracetamol, Salicylate, Digoxin, Tobramycin). Ionised Calcium, Vitamin D, Parathyroid Hormone, Osmolality, Bone Markers, Endocrine Testing (excluding Thyroid). 5-6 Serum/Gel Growth Hormone, ADNA, Gastrin, Electrophoresis, Immunoglobins (IgG, IgM,IgA,IgE), B2 Microglobin, Ceruloplasmin. Infectious Mono,Thyroid Ab, Liver Ab, Rheumatology, Coeliac Ab 5-6 Plasma/Gel UE, LFT, Creatine Kinase, Ca, Mg, Phosphate, Uric Acid, Total Protein, Amylase, Lipids, Bone Profile, Troponin T, Iron Studies, ACE, CRP, TSH, FT4, T3, Cortisol, Vitamin B12, Folate, Ferritin, PSA, Free PSA, CEA, AFP,HCG,CA125, CA19.9,CA153 Cat. No. 363095 / 363093 Draw Volume Mix Times 2.7ml / 1.8ml Cat. No. 367837 Draw Volume 6ml Cat. No. 367954 Draw Volume 5ml Cat. No. 367375 Draw Volume 4.5ml Cat. No. 368857 Draw Volume EDTA (Potassium) Full Blood Count (FBC), ESR, C3/C4, Haemoglobin A1c,Homocysteine, ACTH, Sirolimus, Tacrolimus, Cyclosporin, PCR for CMV, HIV,EBV and Haemochromatosis Genetics. 3ml Mix Times Mix Times Mix One full plasma gel tube is sufficient for all the tests in this group 8-10 Homocysteine and ACTH should be taken onto ice. State time specimen taken. One tube for FBC and ESR. Separate tubes for each of the other tests in this group 8-10 Times Mix Times Cat. No. Mix Draw Volume EDTA Group; Crossmatch Fluoride Oxalate Glucose and Ethanol 8-10 Times Cat. No. 368920 Draw Volume Mix 8-10 Times 2ml Cat. No. 368380 / 368381 Draw Volume Trace Element Zinc, Copper and Selenium - Navy Cap with a red strip on the side of the label. Chromium and Cobalt – Potassium EDTA Navy cap with blue band on tube Mix 8-10 Times 6ml Mix IMPORTANT MIXING GUIDELINES 8-10 Times All BD Vacutainer tubes require immediate mixing following collection. Insufficient mixing can result in inaccurate test results and the need to re-draw. Correct mixing technique is to invert each tube by the recommended number of times shown on the right hand side of the table. ® BD, BD Logo, Vacutainer and Hemogard are trademarks of Becton, Dickinson and Company ©2012 BD. BD Diagnostics - Preanalytical Systems The Danby Building Edmund Halley Road Oxford Science Park Oxford OX4 4DQ Tel: 01865 781603 Fax: 01865 781528