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BHARATI VIDYAPEETH DEEMED UNIVERSITY, PUNE
Revised Syllabus for Ph.D. Entrance Examination in
MICROBIOLOGY
(2015-16)
1.
MICROBIAL SYSTEMATICS - Basic principles and methods of
classification of Bacteria, ( Bergey’s manual of Systematic Bacteriology Edition I
Vol I to IV) Fungi, (The Fungi Vol I to IV), Yeasts (The Yeasts) and Viruses.
Recent trends in Microbial taxonomy, Nature of microbial diversity, Tools of
bioinformatics in microbial systematic.
2.
EXTREMOPHILES - Ecology, Characteristic features with examples,
Applications.
3.
MICROBIAL
METABOLISM
-
Bioenergetics,
Macromolecules
(carbohydrates, proteins, nucleic acids and lipids) - structure, synthesis and
breakdown, Enzymes - characteristics, purification methods and kinetics, Aerobic
and anaerobic respiration, Nitrogen metabolism, Photosynthesis, Membrane
transport, Regulation of metabolism, Metabolomics.
4.
MOLECULAR BIOLOGY AND GENETICS - Prokaryote genome
organization, Eukaryotic genome organization, Recombination, Concept and
methodology of genome mapping. Plasmids (types and uses), Transposable
Elements, Regulation of gene expression.
5. RECOMBINANT DNA TECHNOLOGY - NIH guidelines, Tools, Host
organisms used, Cutting and Joining, Enzymes used, Vectors used, Methods of
construction and Screening of Recombinant Molecules, Applications, Bioethics.
6.
MICROBIAL TECHNOLOGY - Principles of Bioengineering, Bioreactor -
design and operation, operational modes, Fermentation process control, Strain
Improvement, Production of antibiotics, microbial enzymes, amino acids, organic
acids, vitamins, solvents and biomass. Plant and animal tissue culture and
applications, Microbial fertilizers and biocontrol agents. Production of fermented
foods, probiotics, Bioremediation, Microbial Nano biotechnology, Marine
Biotechnology, Regulatory agencies and their role in microbial technology,
Patents and IPR,GMP,HACCP.
7.
AGRICULTURE MICROBIOLOGY - Types of soil, cycles of elements in
nature, factors affecting soil fertility, Plant diseases - pathogens, control and
management, Geomicrobiology, Microbial Ecology.
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8.
POLLUTION CONTROL TECHNOLOGY - Environment and energy
audit, Treatment of waste from different industries (Food, Dairy, Pharmaceutical,
pesticides, fertilizer, dyes, etc.): methods and assessment, Hazardous waste
Management, Bioremediation, Bioaugmentation, Bio-stimulation.
9.
IMMUNOLOGY
-
Types
of
immunity,
Antigen
and
haptens,
Immunoglobulins (structure, classes and diversity), Techniques of visualization of
antigen antibody interactions, T & B cells, Cytokines, Major Histocompatibility
complexes and MHC
molecules, Transplantation immunology,
Antigen
processing and presentation pathways, Activation of humoral and cellular
immunity, Cytokines - types, nature, functions and applications, Immunodeficiency, Autoimmunity, Immuno-suppression, Cancer and immune system,
Hybridoma technology, Hypersensitivity.
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MEDICAL
AND
PHARMACEUTICAL
MICROBIOLOGY
-
Determinants of microbial virulence, toxin and antitoxin assays, Principles of
diagnosis and therapeutics of infectious diseases, Epidemiology of infectious
diseases (determinative, analytical and experimental epidemiology), Mode of
antibiotics acting on cell wall synthesis, cell membrane, protein and nucleic acid
synthesis, Principles of antibiotic assays, Principles of discovery and development
of antimicrobial agents. Hereditary and non-infectious Diseases – Diagnosis and
Control. Pharmaceutical Validation, Vaccines, Diagnosis and Control of Viral
Diseases.
11.
DAIRY AND FOOD MICROBIOLOGY - Milk and milk products,
Quality control in dairy industry, Food borne pathogens, Advanced techniques in
food microbiology - detecting food borne pathogens and their toxins - genetic and
immunological techniques, predictive modeling, Hazard analysis and critical
control system.
12.
RESEARCH METHODOLOGY: BIOSTATISTICS - Data collection
and processing, statistical inference, Designing development and testing of
hypothesis, mean, mode and median, Tests of significance, standard deviation,
Student's t-test, analysis of variance, correlation analysis, regression analysis,
statistical basis of biological assays. Bioinformatics - Overview and scope basic
principles of computing in bioinformatics, Database, retrieval of biological data,
Phylogenetics, sequence annotation. Scientific writing - General aspect, Review
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writing, Reporting practical and project work, writing literature survey, organizing
poster, oral presentations, writing research paper, thesis writing.
13.
SUSCEPTIBILITY TESTING:
A. Use of liquid and solid media, B.
Factors affecting susceptibility testing, CLSI guidelines. Diffusion methods –
Agar dilution technique, gradient plate techniques, E-test, Kirby Bauer, Stokes
method, D. Susceptibility testing for: i) Anti-mycobacterial agents, ii) Antifungal agents, iii) Anti-protozoan agents & iv) Anti-viral agents
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