European Multi Lake Survey Summer 2015 CyanoCOST/ NETLAKE Training School May 2015 Organization: Evanthia Mantzouki, Bastiaan Ibelings Participants: CyanoCOST & NETLAKE Sevilla, CYANOCOST 19/02/2015 Aims of European Multi Lake Survey 1. Quantify the relative role of nutrients (N vs. P) and temperature as drivers of cyanoblooms (mode and type interaction) 2. Assess the function of trophic state, basin type, functional traits of dominant taxa 3. Risk analysis , toxin profiles of all lakes 27 Countries (54% Inclusiveness) 55 Participants ≈ 100s lakes PROTOCOLS • EPA – National Lakes Assessment 2012 • GLEON – TTG Spring Blitz 2013 LAKE SELECTION (NLA Criteria) ≥ 1 ha in total surface area ≥ 1000 square meters of open water ≥ 1 meter in depth • Not saline (due to salt water intrusion or tidal influence) • Not used for aquaculture # Site ID Lake Name Country/ Region Stratification Regime Trophic State Latitude Climate Lake Size Sediment Type Contact Meteorological data (nearest station) Wind speed Air Temperature (°C) Precipitation (daily mm per year) 2. Scum Chlorophyll a Toxins Phytoplankton 1. Historical or Deepest Point T, C profiles Chl fluorescence / Secchi depth DO, pH, PAR P, N Phytoplankton (microscopy, flowcytometry) Micro-, Mesozooplankton Microbes, community composition (preserve for future) Toxins Field Scenario 1. Prepare equipment and supplies for sampling. Calibrate sensors and multi probe meter 2. Lake sampling (Sampling day chosen by each participant, capturing the seasonal maximum) Meteorological data: Wind speed, air temperature and precipitation Profiles of temperature, Chl Fluorescence, DO and pH in Index site Secchi depth 3. Determine depth of sampling (3 depths) Sampling at 3 discreet depths: Chlorophyll a, nutrients, phytoplankton, zooplankton, microbes- community composition, toxins 4. Inspect periphery and spot Scum Sampling surface: Chlorophyll a, phytoplankton, toxins 5. Ensure all samples are labelled and dated correctly 6. Sample preparation 7. Sample analysis 8. Shipping CYANOCOST and NETLAKE Training School 2015 From A to Z: Methods for a successful Multi-lake survey on cyanobacterial blooms Evian Les Bains, France 10-14/May / 2015 Tentative programme • Webinar (April) – Feedback, Protocols, EMLS Preparation needs • Training School • 1st day: • • • • • Phytoplankton (B. Ibelings, University of Geneva) Phyto- Cyano- Bloom problems (H. Paerl, University of N.Carolina) Multi lake surveys (C. Carey, Virginia Tech) Toxins (Miguel Lurling, University of Wageningen) Database management (L. de Senerpont Domis, Netherlands Institute of Ecology) • Equipment and calibration (M. Tercier, University of Geneva) • 2nd day: • A, B, C teams – Field protocols, Sample handling, Microscopy • 3nd day: • A, B, C teams - Field protocols, Sample handling, Microscopy • Introduction to R, Statistical Analysis (C. Carey) Tentative programme • European Multi lake survey – Summer 2015 • Sampling analysis in each group seperately • Microscopy (phyto- & cyano- abundance, biomass, biovolume) • Chlorophyll a • Shipment to dedicated labs: • Toxins - LEAS (Athens) • Flowcytometry – HOPEFULLY! Institute F.A. Forel (Geneva) • Nutrients - ? Christoffersen University of Copenhagen… Name Victoria Kremser Petar Žutinić Maria Antoniou Trine Warming Perlt Kersti Kangro Kerstin Häggqvist Luke Brient Hans Peter Grossart Anastasia Hiskia Andrea Torokne Victor C Perello Paola Buscarinu Jolanta Jekabsone Judita Koreiviene Boris Aleksovskian Petra Visser Justyna Kobos Magdalena Toporowska Joao Morais Zsolt Torok Tamara Dulic Hana Nemova Tina Elersek Ana Garcia Murcia Carmen Cillero Castro William Colom Montero Meryem Beglioglu Jessica Richardson Christine Edwards Training School Participant List Email address [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] Country Austria Croatia Cyprus Denmark Estonia (Tartu) Finland France Germany Greece (Athens) Hungary Ireland (Dundalk) Italy Latvia (Riga) Lithuania Macedonia Netherlands (Amsterdam) Poland (Gdansk) Poland (Lublin) Portugal (Porto) Romania (Tulcea) Serbia (Novi Sad) Slovakia Slovenia Spain (Barcelona) Spain (A Coruna) Sweden Turkey (Ankara) UK (Lancaster) UK (Aberdeen) Action CyanoCOST NETLAKE CyanoCOST CyanoCOST NETLAKE NETLAKE CyanoCOST NETLAKE CyanoCOST CyanoCOST NETLAKE CyanoCOST NETLAKE CyanoCOST CyanoCOST CyanoCOST CyanoCOST CyanoCOST CyanoCOST Neither CyanoCOST CyanoCOST CyanoCOST CyanoCOST Neither NETLAKE NETLAKE CyanoCOST CyanoCOST Suggestions??